The invention discloses a high-flux zebra fish behavior analysis device and method, and aims to solve the problem of compatibility of plate edge
distortion and a multi-type pore plate, a telescopic mechanism is arranged in a shell of the device, an isolation cavity is formed in the shell when the telescopic mechanism is retracted, a micro-
lens array and a
light filter plate are sequentially arranged in the isolation cavity from top to bottom, and the micro-
lens array and the
light filter plate are arranged in the shell. A stimulation module is arranged at the bottom of the
light filter plate, a pore plate placement area is arranged between the stimulation module and the light filter plate, and a universal adaptive bracket for accommodating a standard pore plate and a non-standard pore plate is arranged in the pore plate placement area; a
camera module comprising at least one high-frame-rate camera with a fixed
focal length is correspondingly arranged above the porous plate placing area, the
camera module is connected with a control and analysis module, distortionless track diagrams, heat diagrams and behavior parameters are output online, and the optical mechanical integrated
distortion correction and porous plate combined universal
adaptation structure is used for correcting the
distortion of the porous plate. When the same set of light path is switched between standard plates and non-standard plates of different models, the distortion of the edge view field is automatically compensated.