Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and device for detecting and separating HP (helicobacter pylori) ELISA (enzyme-linked immuno sorbent assay)

A Helicobacter pylori, fully mixed technology, applied in the field of microelectronic medical detection, to achieve the effect of portability, high degree of automation, and simple operation

Inactive Publication Date: 2013-09-04
QIQIHAR MEDICAL UNIVERSITY
View PDF6 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it suffers from similar problems to immunological assays

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and device for detecting and separating HP (helicobacter pylori) ELISA (enzyme-linked immuno sorbent assay)
  • Method and device for detecting and separating HP (helicobacter pylori) ELISA (enzyme-linked immuno sorbent assay)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] See figure 1 , figure 2 , A method for detecting and separating Helicobacter pylori by ELISA, including the following steps:

[0023] (A) Prepare magnetic beads with HP antibodies on the surface, enzyme-labeled antibodies that can react with HP, and enzyme reaction substrates that can react with enzymes;

[0024] (B) Put the prepared magnetic beads, enzyme-labeled antibody, enzyme reaction substrate, gastric juice sample, and cleaning solution into different sample reservoirs on the microfluidic chip, and control the different reservoirs through electric control Liquid flow in

[0025] (C) Mix the magnetic bead antibody and the gastric juice sample thoroughly in the microchannel, and HP will bind to the magnetic bead to form a magnetic bead antigen-antibody complex;

[0026] (D) Fix the magnetic beads in the microchannel, and clean and remove other unbound substances with electric control cleaning liquid;

[0027] (E) Mix the magnetic bead antigen-antibody complex and enzyme-l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method and a device for detecting and separating HP ELISA. According to the method, a prepared magnetic bead provided with HP, an enzyme-labeled antibody, an enzyme reaction substrate, a gastric juice sample and a cleaning fluid are placed in different liquid storage tanks on a micro-fluidic chip by the aid of an electric fluid force, and flow of liquids in the different liquid storage tanks is electrically controlled , so that ELISA is fully and automatically detected; and a detected fluorescence signal is taken as a trigger signal, so that an HP sample is fully and automatically separated. The device comprises a platform structure, the micro-fluidic chip and an electric cabinet, wherein the micro-fluidic chip comprises a glass sheet, a PDMS (polydimethylsiloxane) square plate and a driving electrode, a micro channel, a mixed channel and a detection area are formed in the surface of the PDMS square plate in a photoetching manner by the aid of a soft lithography technology, and the liquid storage tanks are arranged on the PDMS square plate. The device for detecting and separating the HP ELISA has the advantages as follows: the device is small in size, light in weight, convenient to carry and operate and low in construction cost and can be handheld for field detection, thereby facilitating popularization and application.

Description

Technical field [0001] The invention relates to a method and device for detecting and separating Helicobacter pylori by ELISA, in particular to a method and device for fully automatic counting and separating Helicobacter pylori in gastric mucosa samples of gastric juice by ELISA on a microfluidic chip. It belongs to the field of microelectronic medical detection technology. Background technique [0002] Helicobacter pylori (HP) is a unipolar, multiflagella, blunt-ended, spiral-curved bacterium. It often exhibits a typical spiral or arc on the surface of gastric mucosal epithelial cells. Helicobacter pylori infection is chronic active gastritis. , Peptic ulcer, gastric mucosa-associated lymphoid tissue lymphoma and gastric cancer are the main pathogenic factors. Helicobacter pylori has been designated as a class I carcinogen by the World Health Organization / International Agency for Research on Cancer (WHO / IARC). At present, the main methods for detecting Helicobacter pylori inclu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/569G01N33/543G01N21/64
Inventor 张晓杰于秀文
Owner QIQIHAR MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com