Method and device for detecting and separating HP (helicobacter pylori) ELISA (enzyme-linked immuno sorbent assay)

A Helicobacter pylori, fully mixed technology, applied in the field of microelectronic medical detection, to achieve the effect of portability, high degree of automation, and simple operation

Inactive Publication Date: 2013-09-04
QIQIHAR MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method and device for detecting and separating HP (helicobacter pylori) ELISA (enzyme-linked immuno sorbent assay)
  • Method and device for detecting and separating HP (helicobacter pylori) ELISA (enzyme-linked immuno sorbent assay)

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Embodiment Construction

[0022] See figure 1 , figure 2 , A method for detecting and separating Helicobacter pylori by ELISA, including the following steps:

[0023] (A) Prepare magnetic beads with HP antibodies on the surface, enzyme-labeled antibodies that can react with HP, and enzyme reaction substrates that can react with enzymes;

[0024] (B) Put the prepared magnetic beads, enzyme-labeled antibody, enzyme reaction substrate, gastric juice sample, and cleaning solution into different sample reservoirs on the microfluidic chip, and control the different reservoirs through electric control Liquid flow in

[0025] (C) Mix the magnetic bead antibody and the gastric juice sample thoroughly in the microchannel, and HP will bind to the magnetic bead to form a magnetic bead antigen-antibody complex;

[0026] (D) Fix the magnetic beads in the microchannel, and clean and remove other unbound substances with electric control cleaning liquid;

[0027] (E) Mix the magnetic bead antigen-antibody complex and enzyme-l...

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Abstract

The invention discloses a method and a device for detecting and separating HP ELISA. According to the method, a prepared magnetic bead provided with HP, an enzyme-labeled antibody, an enzyme reaction substrate, a gastric juice sample and a cleaning fluid are placed in different liquid storage tanks on a micro-fluidic chip by the aid of an electric fluid force, and flow of liquids in the different liquid storage tanks is electrically controlled , so that ELISA is fully and automatically detected; and a detected fluorescence signal is taken as a trigger signal, so that an HP sample is fully and automatically separated. The device comprises a platform structure, the micro-fluidic chip and an electric cabinet, wherein the micro-fluidic chip comprises a glass sheet, a PDMS (polydimethylsiloxane) square plate and a driving electrode, a micro channel, a mixed channel and a detection area are formed in the surface of the PDMS square plate in a photoetching manner by the aid of a soft lithography technology, and the liquid storage tanks are arranged on the PDMS square plate. The device for detecting and separating the HP ELISA has the advantages as follows: the device is small in size, light in weight, convenient to carry and operate and low in construction cost and can be handheld for field detection, thereby facilitating popularization and application.

Description

Technical field [0001] The invention relates to a method and device for detecting and separating Helicobacter pylori by ELISA, in particular to a method and device for fully automatic counting and separating Helicobacter pylori in gastric mucosa samples of gastric juice by ELISA on a microfluidic chip. It belongs to the field of microelectronic medical detection technology. Background technique [0002] Helicobacter pylori (HP) is a unipolar, multiflagella, blunt-ended, spiral-curved bacterium. It often exhibits a typical spiral or arc on the surface of gastric mucosal epithelial cells. Helicobacter pylori infection is chronic active gastritis. , Peptic ulcer, gastric mucosa-associated lymphoid tissue lymphoma and gastric cancer are the main pathogenic factors. Helicobacter pylori has been designated as a class I carcinogen by the World Health Organization / International Agency for Research on Cancer (WHO / IARC). At present, the main methods for detecting Helicobacter pylori inclu...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543G01N21/64
Inventor 张晓杰于秀文
Owner QIQIHAR MEDICAL UNIVERSITY
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