The invention relates to the technical field of animal models, and particularly discloses a multi-stage
bladder cancer model and a construction method and application thereof. According to the application, specific components of common cigarette extracts popular in the market are identified,
urine components of clinical smokers are collected and detected, the fact that
benzene and NKK exist in the cigarettes and the
urine of the smokers at the same time is successfully found, and the cigarette components
benzene and NKK are selected for
bladder cancer modeling;
benzene and NNK are strong cancerogens existing in tobacco components and
urine of smokers, the content of the components is high, the physiological and
pathological process of bladder cancerization caused by long-term stimulation after the smokers inhale
smoke, enter blood through lungs and form urine through kidneys can be directly simulated, and real life conditions are better met. The multi-stage
bladder cancer model is closer to biological characteristics of bladder
cancer, has specific
exposure factors, and is short in preparation period, high in
tumor formation rate, high in
operability and good in
repeatability.