256 results about "Pharmacopoeia" patented technology

The invention discloses a traditional Chinese medicine decoction piece finished product quality detection and analysis method and system, and belongs to the field of medicines.The method comprises the steps that S1, a compound component database is built, the database integrates pharmacopoeia standards, clinical composition structures, medicinal material source attributes and pharmacodynamic related parameters, and a standard model is built in a multi-level structuring mode; s2, collecting multi-modal map data of the traditional Chinese medicine decoction piece samples; s3, encoding the collected atlas data by adopting a heterogeneous feature extraction algorithm to generate a fusion structure feature vector; s4, inputting the fusion structure feature vector into a multi-channel evaluation model, and carrying out weight comparison, classification judgment and threshold evaluation; and S5, generating a quality detection report. The method has the beneficial effects that high-precision quality detection combining multi-modal fusion, intelligent discrimination and traceability visualization is realized, and the quality consistency evaluation and risk identification capability of the traditional Chinese medicine decoction pieces is effectively improved.

The invention belongs to the technical field of in-vitro cultivation of calculus bovis, and particularly relates to a method for in-vitro cultivation of calculus bovis. According to the invention, firstly, the escherichia coli is subjected to bile domestication and ray induced mutation, a high-activity oxgall-resistant bezoar production engineering bacterium E.coli-NH01 is obtained through screening, a method for in-vitro cultivation of bezoar by using the engineering bacterium E.coli-NH01 is provided, the prepared bezoar meets the pharmacopoeia standard, the production efficiency of in-vitro cultivation of bezoar is improved, and the production cost is reduced. And the production cost is reduced, the overall forming period is shortened, and the key that precious traditional Chinese medicinal materials are popular can be achieved.

The invention relates to the field of computer vision, in particular to an intelligent identifying and sorting method and system for traditional Chinese medicinal materials. Based on a multispectral and microscopic vision fusion engine, millisecond-level authenticity identification of traditional Chinese medicinal materials is realized through sub-pixel-level feature extraction and cross-modal knowledge distillation, and similar medicinal material confusion is thoroughly eliminated by combining PointRend point cloud segmentation and pharmacopoeia map confrontation verification; a rotary YOLO-MoE multi-angle defect detection mechanism is innovated, a zero-adulteration quality control closed loop with zero manual intervention is achieved under the assistance of a self-adaptive guide execution unit, and a map database is continuously upgraded synchronously through a dynamic federated learning mechanism. The technical bottleneck that an existing traditional Chinese medicine identification system depends on artificial experience and is weak in authenticity identification capability is broken through, and the key problems of confusion of medicinal materials with similar forms caused by single dimension of semi-automatic pharmacy visual identification, missing detection of defective products caused by no quality control mechanism and the like are thoroughly solved; and the medicinal material identifying and sorting process is improved to a stable, reliable, self-adaptive and cooperative intelligent level.

The invention discloses a human albumin binding peptide 1E3 and application of the human albumin binding peptide 1E3 in promoting purification of human albumin, and belongs to the technical field of polypeptides. The human albumin binding peptide comprises an amino acid sequence as shown in SEQ ID NO. 1; and / or an amino acid sequence of a fusion protein with the same function, which is obtained by connecting tag protein to the N terminal and / or C terminal of the amino acid sequence as shown in SEQ ID NO.1. The human albumin binding peptide has extremely high affinity with human albumin and can be used for separating and purifying a human albumin solution, and the purity of the purified human albumin far exceeds the pharmacopoeia standard and can reach 99.99% or above. The method is good in safety and stable in process, and has a wide application prospect in the aspect of separation and purification of the human albumin.

The invention belongs to the technical field of separation of drug intermediates, and particularly relates to a control method for a process impurity 2-ethyl valeric acid (EP-B) in preparation of valproic acid or sodium valproate by a diethyl malonate method. According to the method disclosed by the invention, the content of 2-ethyl-2-propylmalonic acid process impurities in dipropylmalonic acid is controlled, so that the content of 2-ethylvaleric acid (EP-B) which is a process impurity in valproic acid or sodium valproate is accurately controlled; adding a polar solvent into the dipropylmalonic acid crude product, heating, pulping for a certain time, cooling, filtering and drying to obtain a high-purity dipropylmalonic acid refined product; high-purity valproic acid (RRT is equal to 1.00) is prepared through decarboxylation of the dipropyl malonic acid fine product, and the purity is 99.779%; 0.020% of 2-ethyl valeric acid (RRT is equal to 0.90); and valeric acid (RRT = 0.77), 0.039%. The valproic acid product quality meets the requirements of European Pharmacopoeia. According to the method, the problems of quality control and process evaluation of valproic acid or sodium valproate prepared by a diethyl malonate method are solved.

The invention belongs to the technical field of separation of drug intermediates, and particularly relates to a method for preparing a high-purity dipropylmalonic acid refined product by adding a polar solvent into a dipropylmalonic acid crude product, heating, pulping for a certain time, cooling, filtering and drying. Decarboxylating the refined dipropylmalonic acid product to obtain high-purity valproic acid; distilling the filtrate to recover the polar solvent to obtain 2-methyl-2-propylmalonic acid, and carrying out decarboxylation on the 2-methyl-2-propylmalonic acid to obtain 2-methylpentanoic acid; 2-methylvaleric acid is used as a perfume additive. According to the method disclosed by the invention, the process impurity 2-methyl-2-propylmalonic acid in dipropylmalonic acid is separated, and the process impurity 2-methylvaleric acid (EP-L) in valproic acid or sodium valproate is precisely separated; the quality of the valproic acid product prepared by the method disclosed by the invention meets the requirements of European Pharmacopoeia EP-11 (2023). According to the invention, the problems of quality control and process evaluation of valproic acid or sodium valproate prepared by a dimethyl malonate method are solved.

The invention provides a traditional Chinese medicine decoction piece quality evaluation method based on big data, and relates to the technical field of data processing, and the method comprises the steps: extracting texture direction consistency data from texture dense region detection points of a central region, and extracting structural symmetry data from wrinkle region detection points; comparing the color gradient change data with a preset standard color gradient threshold to generate a first difference index, comparing the texture direction consistency data with a historical qualified batch texture direction distribution range to generate a second difference index, and comparing the structural symmetry data with a pharmacopoeia form standard to generate a third difference index; and calculating a quality evaluation value based on the first difference index, the second difference index and the third difference index in combination with the initial quality deviation risk value, and obtaining a quality grade judgment result of the traditional Chinese medicine decoction pieces according to an interval matching relationship between the quality evaluation value and a preset risk threshold. According to the invention, the evaluation accuracy can be improved.

The invention discloses a processing method of wine-processed rhizoma polygonati, which belongs to the technical field of traditional Chinese medicine processing, and comprises the following steps: selecting rhizoma polygonati, cleaning, slicing and drying; spraying an acidic compound solution, steaming for the first time, preserving heat, moistening, and collecting a steaming solution; after finishing and slicing, spraying catalytic compound liquid prepared from steaming liquid and auxiliary materials, steaming for the second time, and preserving heat and moistening; then steaming at normal pressure for the third time; and finally, pre-drying at low temperature, heating and finally drying, and inspecting to obtain a wine rhizoma polygonati finished product. According to the invention, a segmented precise regulation and control system is constructed, so that the used functional additive can be completely removed, and the purity and safety of the product are guaranteed; the compound liquid proportion, spraying parameters and steaming key indexes are defined, systematic control standards are formed, the problem of quality fluctuation of traditional processing is solved, the product appearance and taste are better, the key indexes stably exceed the pharmacopoeia standard, and upgrading from experience and technology to standardized production is achieved.

The present invention provides a non-distillation injection water preparation system, the non-distillation injection water preparation system comprises a pure water production system and an injection water production system connected to each other, the pure water production system comprises a purification module, a first treatment module and a first storage module, untreated raw material water in an external feed source is purified by the purification module and the first treatment module and then enters the first storage module, the injection water production system comprises a second treatment module, a filtering module and a second storage module, and purified water purified by the pure water production system flows out of the first storage module; and the water enters the second storage module through the second processing module and the filtering module. According to the non-distillation injection water preparation system, purified water and injection water can be produced at the same time through a short non-distillation process, the process preparation standard of pharmacopeia is met, the water using process at the rear end of production can be effectively simplified, and the investment cost and the operation cost of the whole system are reduced.

The application discloses a preparation method of fenbendazole impurity C, wherein the fenbendazole impurity C is impurity C in the European Pharmacopoeia (EP), and the compound has a molecular formula of C 27 H 23 N5O2S2, the application takes 2-nitro-5-phenylmercaptoaniline as raw material, realizes the preparation of the fenbendazole impurity C through a four-step reaction in the following route, the preparation method of the impurity compound is easy to obtain raw materials, simple to operate, reasonable in process design, relatively mild in reaction condition, high in yield, and can realize industrial production, the prepared fenbendazole impurity C can be used as a control sample for qualitative and quantitative research of impurity C in fenbendazole quality research, the content of the fenbendazole impurity C is controlled, so that the quality of the crude drug is ensured.

The invention relates to an under-forest mountain ginseng and garden ginseng identification method based on a ginsenoside proportion, and belongs to the field of traditional Chinese medicinal material identification methods. The method specifically comprises the following steps: determining the contents of ginsenosides Rg1, Re and Rb1 of ginseng monomers by adopting a high performance liquid chromatography, calculating the Rb1 / (Rg1 + Re) proportion characteristic, and identifying the understory ginseng and the garden ginseng. The method has the advantages of being small in sample dosage, simple, high in method precision and repeatability and the like, the contents of the three kinds of ginseng monomer saponin specified by the 2020 edition Chinese Pharmacopoeia are measured, the proportion of the three kinds of ginseng monomer saponin can be calculated to serve as an identification index of the understory ginseng and the garden ginseng, and it is stipulated that the proportion Rb1 / (Rg1 + Re) of the ginseng monomer saponin in the understory ginseng is larger than 0.6, and the proportion Rb1 / (Rg1 + Re) of the ginseng monomer saponin in the garden ginseng is smaller than 0.6.

The invention belongs to the technical field of medicine synthesis, and discloses a preparation method of a sodium nitroprusside raw material medicine. According to the method, weak acid is adopted for catalysis, the effect of remarkably reducing generation of toxic substances such as cyanide and nitrogen dioxide is achieved by controlling the material dripping speed and the dripping pH, the reaction is mild, the technological operation is safe and controllable, and industrial production of the sodium nitroprusside raw material medicine is facilitated. According to the preparation method of some examples, silica gel filtration is used for post-treatment, the residual risk of impurities such as sodium ferricyanide, ferric ferrocyanide and ferric ferricyanide is reduced, and the purity of the raw material medicine is larger than or equal to 99.9% and is far superior to the requirements of pharmacopoeia of various countries.

The application belongs to the technical field of medicine synthesis, and particularly relates to a preparation method of methoxychlorpromazine monohydrochloride monohydrate. The method comprises the following steps: step 1, methoxychlorpromazine base is dissolved in a polar solvent, and hydrochloric acid is added to obtain a mixed solution; step 2, a poor solvent of methoxychlorpromazine monohydrochloride monohydrate is added to the mixed solution, crystallization is carried out, and separation is performed, and methoxychlorpromazine monohydrochloride monohydrate is obtained; the poor solvent is selected from one or a mixture of two or more of isopropyl alcohol, n-butyl alcohol, acetone, butanone, ethyl acetate, butyl acetate, dichloromethane, trichloromethane, toluene, methyl tert-butyl ether, isopropyl ether and diethyl ether. The method has the advantages of simple process, good repeatability, easy operation, high yield, product purity of more than 99.8%, complete compliance with the requirements of European Pharmacopoeia 9.0 and US Pharmacopoeia USP43, and suitability for industrial production.

The traditional Chinese medicine comprises the following raw materials in parts by weight: 15g of codonopsis pilosula, 10g of rhizoma acori graminei, 10g of processed polygala tenuifolia, 15g of rhizoma smilacis glabrae, 12g of raw dens draconis, 10g of astragalus membranaceus, 9g of divaricate saposhnikovia root, 10g of bighead atractylodes rhizome, 6g of raw liquorice, 9g of ephedra, 9g of almond, 15g of gypsum, 9g of cassia twig, 9g of rhizoma alismatis, 10g of radix bupleuri, 6g of scutellaria baicalensis, 9g of ginger processed pinellia, 9g of ginger, 9g of aster tataricus, 9g of flos farfarae, 9g of blackberry lily, 3g of asarum, 10g of parched white peony root, 9g of immature bitter orange and 9g of pericarpium citri reticulatae. According to the traditional Chinese medicine for detoxifying and clearing away the lung-heat and the decocting method thereof, the prescription medicine is designed from integrity, prevention and treatment are carried out at the same time, all the prescription medicines adopt non-toxic and harmless top-grade traditional Chinese medicine raw materials recorded in Pharmacopoeia, medicinal materials with bitter, cold and severe nature and taste are reduced as much as possible, damage to yang qi in a body is avoided, and the traditional Chinese medicine is safe and reliable. Therefore, the traditional Chinese medicine composition can meet the requirements of preventing and treating pneumonia in the aspects of safety, reasonability and effectiveness.

The invention belongs to the technical field of liquid phase detection, and particularly relates to a quality control and detection method for lumbricus soaked with liquorice. According to the method, methanol water is used as a mobile phase, the content of hypoxanthine and inosine in the lumbricus glycyrrhiza is detected by high performance liquid chromatography, and the method has important significance on quality control of a lumbricus glycyrrhiza product. The detection method disclosed by the invention is good in system applicability, precision, reproducibility, recovery rate and stability, and meets the requirements of Chinese Pharmacopoeia, Chinese Pharmacopoeia, appendix of Chinese Pharmacopoeia, Chinese appendix of Chinese Pharmacopoeia, Chinese Pharmacopoeia, Chinese

The invention relates to a formula and a preparation method of compound desert cistanche granules, the compound desert cistanche granules are prepared by adding flavor on the basis of Qingzheng dialectical record yang-warming soup, the raw materials comprise desert cistanche, bighead atractylodes rhizome, monkshood, Chinese angelica, cynomorium songaricum, fried semen raphani and perillaseed, and the total weight of a single bag is 7g. The production process comprises the key steps of pretreatment of medicinal materials, independent first decoction of radix aconiti carmichaeli for detoxification, extraction of semen raphani and perillaseed volatile oil, inclusion of beta-cyclodextrin, combined decoction and concentration of all the medicinal materials, dry granulation and the like, and the technical effects that granules are instantly dissolved within 45 + / -5 seconds and the transfer rate of crude drugs is 91.2% are achieved by optimizing the proportion of auxiliary materials and process parameters. The quality of the finished product meets the requirements of Chinese Pharmacopoeia, the content of aconitine is less than or equal to 0.02%, the total number of microbial limit aerobic bacteria is less than or equal to 100 CFU / g, the problems that a traditional compound cistanche deserticola preparation is slow in dissolution speed, unstable in toxicity control and non-standard in production process are solved, and the method is suitable for large-scale industrial production.

The invention discloses a method for detecting 5 '(E)-VPA phosphoramidite monomer related substances, and belongs to the technical field of liquid chromatography detection. Related substances comprise 5 '-(E)-VP-2'-OMe-A (Bz), 5 '(Z)-VPA, 5'-(E)-VP-2 '-OMe-A-CE-P and 5' (E)-VPA-OX, and chromatographic detection conditions comprise that the column temperature is 23-27 DEG C, a stationary phase of a chromatographic column is octadecyl bonded silica gel, a mobile phase A is an ammonium acetate solution, a mobile phase B is acetonitrile, gradient elution is performed, and the like. According to the method, four related substances in the 5 '(E)-VPA phosphoramidite monomer are effectively separated through specific liquid chromatography conditions, the system adaptability, specificity, linearity and range, detection limit, quantitation limit and accuracy verification of the method all meet acceptable standards of pharmacopoeia, and detection of multiple related substances can be completed within a short time; and a reliable means is provided for detecting and controlling the quality of the 5 '(E)-VPA phosphoramidite monomer.

The invention relates to a preparation process for producing water for injection based on a hollow fiber deoxidation membrane, and belongs to the technical field of preparation of water for injection medicines. According to the preparation process of the water for injection, dissolved oxygen in a water body can be stably removed, reproduction of microorganisms, bacteria and the like in the water can be effectively restrained by strictly controlling the content of oxygen and carbon dioxide in the water, meanwhile, due to removal of carbon dioxide in the water body, the conductivity of the water body is further reduced, the pH value of the finished water is stabilized to be 6.5-7.5, and the water quality is improved. The related industrial standards of the water for injection are met, and the content of microorganisms and endotoxin meets the strict requirements of the 2025 edition pharmacopoeia. Compared with a traditional distillation method, the membrane method integration process does not need to consume a large amount of industrial steam, energy consumption can be remarkably reduced, and the operation cost is reduced; by optimizing the position of the hollow fiber deoxidation membrane device, the water quality and the storage stability of the water for injection can be improved, and the production cost and the maintenance cost can be reduced.

This invention relates to the field of pharmaceutical technology and discloses a method for improving the clarity and color of oxybuprocaine hydrochloride solution, comprising the following steps: S110: Oxoplasmin is added to a reactor, and an organic solvent is added while stirring to control the reaction system at 0-10°C; an antioxidant is added. S120: Hydrochloric acid is slowly added in batches to the temperature-controlled reaction system. After the addition is complete, the temperature is controlled at 0-10°C for 2-4 hours, then the temperature is raised to 40-50°C for 2-4 hours, and activated carbon is added. S130: The mixture is filtered while hot, and solvent is slowly added dropwise to the filtrate until the product precipitates. After slurrying at 25-35°C for 2 hours, the mixture is filtered, and the filter cake is vacuum dried in a vacuum drying oven at 40-50°C for 12-16 hours to obtain oxybuprocaine hydrochloride. This invention provides a novel method for obtaining oxybuprocaine hydrochloride by adding antioxidants and activated carbon in the salt formation step. The clarity and color of the resulting product solution meet pharmacopoeia requirements.

The invention discloses a physalis alkekengi calyx extract effervescent tablet as well as a preparation process and quality evaluation thereof. The effervescent tablet comprises the following extracts and auxiliary materials in percentage by weight: 7-12% of physalis alkekengi calyx extract, and the auxiliary materials comprising 50% of total acid and alkali (the ratio of acid to alkali is 0.6: 1-1.4: 1), 5-15% of a lubricant, 1-5% of a sweetening agent, 2-10% of an adhesive and the balance of a filling agent, totaling 100%. The invention provides a preparation method of the physalis alkekengi calyx extract effervescent tablet powder by a direct tabletting method, and the process comprises the following steps: uniformly mixing and grinding a lubricant and sodium bicarbonate, adding the ground citric acid, the physalis alkekengi calyx extract, an adhesive, a sweetening agent and a filling agent, uniformly mixing, and tabletting. After the process is optimized, the disintegration time limit of the obtained effervescent tablets meets the requirements of pharmacopeia, and the tablet weight difference is qualified. The method is simple, convenient, feasible, stable and reliable, and the prepared effervescent tablets are rapid in disintegration, good in taste and stable in quality and have high clinical application value and market prospects.

The invention discloses a method for detecting related substances in testosterone enanthate by high performance liquid chromatography. A mobile phase A and a mobile phase B are used as mixed mobile phases for gradient elution; the mobile phase A is water; the mobile phase B is acetonitrile; the volume ratio of the mobile phase A to the mobile phase B is (5%-70%): (30%-95%); a chromatographic column adopted by the high performance liquid chromatography method is Agilent ZORBAX Eclipse XDB-C8, and the detection method is characterized in that the detection method is simple and easy to implement. According to the method, the testosterone enanthate and the related substances thereof are separated and detected by screening a stationary phase chromatographic column and optimizing a mobile phase elution mode and an elution component ratio, and compared with a method in European Pharmacopoeia, after the chromatographic column is subjected to gradient elution, not only is the retention time of an impurity D prolonged, but also the quality of the impurity D is improved. And the separation degree of the impurity F and the impurity G is increased, so that the elution capacity and the separation degree among the impurities are enhanced.

The invention provides a preparation method of trospium chloride. The preparation method of trospium chloride comprises the following steps: dissolving a trospium chloride crude product in a mixed solution of methanol and ethanol, and filtering; and (2) adding methyl tert-butyl ether into the filtrate obtained in the step (1), fully and uniformly mixing, cooling, crystallizing, filtering and drying to obtain trospium chloride. The preparation method is simple and feasible, the target product yield is high, impurities such as the product impurity C can be effectively controlled, the solvent residue content is low, the purity is high, the clarity can meet the EP pharmacopoeia standard, and the industrial application prospect is good.

The invention belongs to the technical field of purification of compounds, and particularly relates to sesame oil with a low peroxide value and a low anisidine value and a preparation process of the sesame oil. The peroxide value of the sesame oil with low peroxide value and anisidine value is less than or equal to 1, and the anisidine value is less than or equal to 2. The invention provides sesame oil with low peroxide value and low anisidine value and a purification method thereof, i.e., industrial-grade sesame oil is used as a raw material, and refined, filter-pressed and other processes are performed to prepare a sesame oil pure product. The sesame oil is purified by a refining method, and the quality of the obtained sesame oil meets the index requirement of the peroxide value of the sesame oil in the 2025 edition of Chinese pharmacopoeia. The refining process is simple, the purification effect is good, and the yield of the obtained product is 90-95%.

The invention provides a nerve soothing and mind stabilizing pill and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine preparations. The preparation method comprises the following steps: cleaning, infiltrating, cutting, drying, performing high-pressure steam sterilization on genuine medicinal materials such as poria cocos, poria with hostwood, ginseng, polygala tenuifolia, rhizoma acori graminei and the like in a formula of the mind-tranquilizing and mind-tranquilizing pill, mixing and crushing at low temperature to obtain fine powder, adding refined honey to prepare pills, and finally coating with cinnabar powder to obtain the mind-tranquilizing and mind-tranquilizing pill. The contents of polygala-ketone III, 3, 6 '-dimustard acyl sucrose, ginsenoside Rb1, beta-asarone, dehydrotumoic acid, pachymic acid and calcium salt in the prepared nerve-soothing and mind-stabilizing pill are high, the loss of index components in the pelleting process is small, and the dissolution time limit and microbial limit of the pill meet the requirements of Chinese Pharmacopoeia of 2025 edition. The preparation process of the mind-tranquilizing and mind-stabilizing pill is stable, and the prepared mind-tranquilizing and mind-stabilizing pill can effectively improve cognitive impairment, inflammation and oxidative stress injury caused by insomnia.

The invention belongs to the technical field of pharmaceutical chemistry, and provides a tranexamic acid crystal and a preparation method and application thereof, the tranexamic acid crystal has characteristic peaks in an X-ray powder diffraction pattern under Cu-K alpha radiation when diffraction angles 2 theta are 10.52 + / -0.2 degrees, 17.54 + / -0.2 degrees, 17.90 + / -0.2 degrees, 20.86 + / -0.2 degrees and 21.24 + / -0.2 degrees. The tranexamic acid crystal is of a novel crystal structure, is high in purity and good in stability, can simultaneously meet EP11.0, USP-NF2025 and tranexamic acid quality standards of the current edition of Chinese Pharmacopoeia, can be kept stable under severe conditions of high temperature, high humidity, strong acid, strong alkali, illumination, oxidation and the like, and is convenient to produce and store. The preparation method of the tranexamic acid crystal is simple to operate, the used crystallization reagent is safe, the production cost is low, and industrial production is easy.

This invention provides a method for preparing rocuronium bromide injection. By optimizing the conventional moist heat sterilization process, specifically by increasing the initial temperature of the heating process and decreasing the initial temperature of the cooling process, the overall exposure time of the injection to high temperatures is significantly shortened while maintaining the sterilization time at 121°C, thus significantly improving the quality stability of rocuronium bromide injection. Multiple batches of samples from this invention have met the related substance limits for rocuronium bromide injection as specified in the Chinese Pharmacopoeia, after accelerated stability and long-term stability studies.

The invention discloses a method for detecting the content of collagen in collagen-hyaluronic acid cross-linked gel. The detection method comprises the following steps: testing the content of collagen in collagen-hyaluronic acid cross-linked gel by adopting a folin-phenol method in combination with an external standard method, specifically, mixing the gel and an alkaline copper test solution for reaction at the temperature of 50-85 DEG C to prepare a test solution; wherein the concentration of sodium hydroxide in the alkaline copper test solution is 50-100 mg / mL; the concentration of the gel in the test solution is 0.02-0.1 g / mL; and mixing the test solution with the folin-phenol test solution, detecting an absorption value by using an ultraviolet and visible spectrophotometer, and calculating the collagen content in the gel through a standard curve. According to the collagen content detection method, a folin-phenol method in pharmacopoeia is improved, the operation is simple, and the detection accuracy, repeatability and durability all meet the requirements.

The invention discloses a human albumin binding peptide 1A3 and application thereof in purification of human albumin, and belongs to the technical field of polypeptides. The amino acid sequence of the human albumin binding peptide comprises an amino acid sequence as shown in SEQ ID NO. 1; and / or an amino acid sequence of a fusion protein with the same function, which is obtained by connecting tag protein to the N terminal and / or C terminal of the amino acid sequence as shown in SEQ ID NO.1. The human albumin binding peptide has extremely high affinity with human albumin and can be used for separating and purifying a human albumin solution, and the purity of the purified human albumin far exceeds the pharmacopoeia standard and can reach 99.99% or above. The method is good in safety and stable in process, and has a wide application prospect in the aspect of separation and purification of the human albumin.

The invention provides a planting method for increasing the content of effective components of liquorice, and belongs to the technical field of Chinese herbal medicine planting. The planting method comprises the steps that 1.6-2.4 kg of mineral source humic acid fertilizer is applied per mu from June to August, and 1.6-2.4 kg of microelement water-soluble fertilizer is applied per mu after 7-10 days; the trace element water-soluble fertilizer contains more than or equal to 5% of zinc, manganese, calcium, magnesium, iron and molybdenum. The soil microenvironment is improved through humic acid, root development and secondary metabolism precursor accumulation are promoted, photosynthesis and secondary metabolism pathways are accurately activated in combination with microelements, the content of glycyrrhizic acid and liquiritin is synergistically increased, effective components of the licorice medicinal material meet the requirements of Chinese Pharmacopoeia, and ecological management and medicinal material efficient production value are achieved.

The application belongs to the technical field of biological medicine, and relates to synthesis of a medicine, in particular to a preparation method of picrosulfa sodium. Phenol and 2-pyridine formaldehyde are used as raw materials, trifluoroacetic acid and formic acid are used as reaction solvents, concentrated sulfuric acid is added dropwise, 4,4'-(2-pyridine methylene)-bisphenol crude product is obtained after the reaction is completed, the 4,4'-(2-pyridine methylene)-bisphenol crude product is refined to obtain 4,4'-(2-pyridine methylene)-bisphenol pure product; the 4,4'-(2-pyridine methylene)-bisphenol pure product is subjected to a sulfonation reaction by using sulfur trioxide pyridine complex, and then subjected to an alkali neutralization reaction to obtain picrosulfa sodium crude product, and the picrosulfa sodium crude product is refined to obtain picrosulfa sodium pure product. The application can reduce the generation of isomers, improve the yield, is suitable for industrial production, and the prepared picrosulfa sodium meets the requirements of the quality standard of the European Pharmacopoeia.