302 results about "High-performance liquid chromatography" patented technology

The invention relates to the technical field of ecological system service function evaluation. The invention relates to a frequency offset correction algorithm in HPLC (High Performance Liquid Chromatography) + HRF (High Radio Frequency) dual-mode communication. The method comprises the following steps: S1, collecting phase sequence data from an HPLC communication link, collecting frequency offset estimation data and corresponding confidence data from an HRF communication link, and adaptively adjusting a preset detection threshold value of the phase sequence data according to the confidence data; s2, performing pulse abnormal sample point detection and elimination on the phase sequence data according to the adjusted preset detection threshold value; according to the method, the detection threshold is dynamically adjusted through the confidence data, so that the anomaly detection process can be matched with the current interference environment in real time, the integrity of the phase sequence data is maintained, and the problem of insufficient adaptability of a fixed detection threshold in an electromagnetic interference dynamic change scene is solved; and a more reliable phase data basis is provided for subsequent interpolation compensation and frequency offset estimation, so that the frequency offset correction precision of the dual-mode communication system is improved.

The invention discloses an oligopeptide from undaria pinnatifida and application thereof. The amino acid sequence of the oligopeptide is YRKD, and the molecular weight is 580.63 g / mol. The polypeptide is identified from undaria pinnatifida through a high performance liquid chromatography technology for the first time, and an unreported oligopeptide capable of inhibiting tyrosinase and cooperating with vitamin C to synergistically inhibit tyrosinase is screened out. The oligopeptide derived from undaria pinnatifida is synthesized by a solid phase method, has the characteristics of safety, no toxicity, good water solubility and good stability, and is combined with vitamin C to generate a synergistic effect and synergistically increase the inhibition effect on tyrosinase, so that the oligopeptide can achieve a better tyrosinase inhibition effect at a lower and more stable concentration; the use concentration of the oligopeptide can be greatly reduced, the stability is enhanced, and the oligopeptide can be widely applied to preparation of cosmetics, medicines, health-care products or food additives, and has important economic value and social significance.

The invention discloses a method for extracting ecdysterone from cyanotis arachnoidea by using a deep-eutectic solvent, and belongs to the technical field of ecdysterone extraction. The method comprises the following steps: mixing a deep-eutectic solvent which takes choline chloride and a hydrogen bond donor material as raw materials with water, extracting ecdysterone in roots and stems of Cyanotis arachnoidea to obtain an ecdysterone crude extract, precipitating the ecdysterone crude extract by adding water, filtering, and adsorbing ecdysterone by using macroporous resin. Adding water for elution to obtain a deep-eutectic solvent-water mixed solution, and recovering under reduced pressure to obtain an extraction solvent for cyclic utilization; and eluting by using alcohol with different concentrations to obtain an ecdysterone crude extract, and purifying the crude extract to obtain high-purity ecdysterone. Compared with a conventional extraction method, the process method adopted by the invention has the advantages that the process is simple, efficient, green and environment-friendly, the used eutectic solvent can be recycled, the loss caused by heating volatilization in the extraction process is avoided, the ecdysterone extracted by the method is pure white, the content of ecdysterone can reach 90% or above after simple purification, and the method is suitable for industrial production. After re-purification, the purity of ecdysterone detected by high performance liquid chromatography is up to 98% or above.

The invention discloses an efficient HPLC dual-mode communication method and system, and relates to the technical field of communication analysis, and the method comprises the steps: obtaining service request data and channel state data in a dual-mode network; performing service priority classification based on the service request data so as to obtain service priority data, and performing channel quality evaluation based on the channel state data so as to obtain channel quality data; performing service and channel dynamic matching analysis according to the service priority data and the channel quality data to obtain dynamic matching strategy data, and performing scheduling queue optimization based on the dynamic matching strategy data to obtain optimized scheduling queue data; and performing a dual-mode channel switching decision according to the optimized scheduling queue data, generating channel switching decision data, performing service data transmission control through the channel switching decision data, obtaining service data transmission control data, and further realizing dual-mode communication according to the service data transmission control data. The dual-mode communication method has the effect of improving the dual-mode communication efficiency.

The invention provides a chemical or enzymic method regenerated PET and PTA screening method, which comprises the following steps: pretreating a PET or PTA sample 1, and measuring the content of p-toluic acid, the content of p-carboxybenzaldehyde and the content of m-terephthalic acid by using high performance liquid chromatography; the method comprises the following steps: pretreating a PET or PTA sample 2, pretreating by using a high-temperature pyrolysis method, and measuring an isotope ratio of oxygen-18 to oxygen-16 by using an isotope ratio mass spectrometer; the method comprises the following steps: calculating a cleanliness index, an oxy-18 index and a bottle flake index by using a formula based on a test result, and classifying PET and PTA samples through a decision tree according to the indexes; combining and mixing the PET and PTA products based on the classification result and the requirements of downstream customers on the regeneration method and the regenerated material content; the method is convenient, accurate and reliable, the classification result is objective, the screening efficiency of PET and PTA regenerated by a chemical or enzyme method can be improved, and the economic cost is saved.

The invention belongs to the technical field of medicine detection, and particularly relates to a method for determining the content of impurities in OAB-14 by using high performance liquid chromatography. The method comprises the following steps: preparing a test sample labeling solution, a test sample solution and a contrast solution of OAB-14, respectively injecting the test sample labeling solution, the blank solution, the contrast solution and the test sample solution into a liquid chromatograph, recording chromatograms, and calculating according to a main component self-contrast method to obtain the content of impurities in the test sample solution; wherein the impurities are one or more of an impurity A, an impurity B, an impurity C, an impurity D, an impurity E or an impurity F. According to the method, the impurities in the OAB-14 can be qualitatively and quantitatively measured at the same time, the stability and the reproducibility are good, the separation degree between a main peak and each impurity peak is good, the precision is high, and an analysis means is provided for quality control of the OAB-14.

The invention discloses an HPLC (High Performance Liquid Chromatography) signal processing method based on bit loading, and belongs to the technical field of power line carrier communication. The HPLC signal processing method based on bit loading comprises the following steps: reading bit data from a channel interleaving module, and sequentially writing the bit data into a first bit buffer queue according to a preset read bit number of the channel interleaving module; in the bit loading mode, aiming at the modulation mode corresponding to each subcarrier, determining a target bit number required to be filled by each subcarrier; different modulation modes correspond to different target bit numbers; and sequentially reading the bit data of the target bit number from the first bit buffer queue according to the write-in sequence, and mapping the bit data read as required to the corresponding subcarrier for modulation and transmission. According to the invention, rate efficient matching of data processing in a bit loading mode and data transmission without subcarrier cross-physical blocks are realized.

The invention discloses a dual-mode communication chip, and belongs to the technical field of communication. The dual-mode communication chip comprises an HPLC (High Performance Liquid Chromatography) signal transmitting end and an HPLC signal receiving end; the HPLC signal transmitting end is configured as a coding module and is used for coding the load data to obtain coded load data; the first storage module is used for storing the coded load data; the channel interleaving module is used for carrying out interleaving processing on the bit data read out from the first storage module; the first bit buffer queue is used for sequentially writing the bit data from the channel interleaving module according to a first bit number and sequentially reading the bit data according to a writing sequence according to a second bit number; and the bit mapping module is used for mapping the bit data from the first bit buffer queue to each subcarrier with a second bit number. According to the invention, rate efficient matching of data processing in a high-order modulation mode and data transmission without subcarrier cross-physical blocks are realized.

The application discloses a kind of ruthenium grafting manganese aluminum hydrotalcite and catalytic method for C02 hydrogenation preparation formic acid, belong to catalyst technical field, in the application, under the optimized reaction condition, with methanol:water mixture as solvent (5:1), total pressure is 60bar, 60 DEG C, reaction 24 hours, with temperature controller to be heated to the temperature required for reactor.When the temperature inside the reactor reaches the specified temperature, start stirring at a fixed stirring speed (200-800 revolutions / min), after the required reaction time, stop stirring and heating, and allow the reactor to cool to room temperature. The hydrogenation products were analyzed by high performance liquid chromatography, the conversion number (TON) of formic acid reached more than 8000; RuMnAl-LDH was prepared by coprecipitation method, and RuMnAl hydrotalcite catalyst was obtained by heating and activation in a muffle furnace. The preparation process is simple, and the raw materials are easy to obtain. The RuMnAl-LDH catalyst prepared by the application has good catalytic activity and stability in the catalytic hydrogenation of formic acid to formic acid reaction, and has wide application prospect.

The invention relates to the technical field of traditional Chinese medicines, in particular to a processing method and a quality control method for toxicity reduction and efficacy enhancement of radix cynanchi bungei. According to the method, the content of alangiine, the content of ellagic acid, the common peak area of characteristic peaks and the content of water-soluble extracts in the wine-roasted berberis scenting decoction pieces are measured by adopting a high performance liquid chromatography (HPLC) method, and subjective assignment is carried out on the characters of the wine-roasted berberis scenting decoction pieces by combining Delphi evaluation to serve as evaluation indexes; the method comprises the following steps: determining the weight coefficient of each index by adopting an analytic hierarchy process-index repeatability correlation mixed weighting method (AHP-CRITIC), and screening the optimal wine roasting process of the Zhuang medicine Tibetan scene by combining the three factors of the frying temperature, the frying time and the yellow wine amount with star point design (CCD)-effect surface (RSM) according to the coefficients. And finally determining the optimal process of stir-frying the radix berberidis with wine as follows: the stir-frying temperature is 150 DEG C, the stir-frying time is 16min, the amount of yellow rice wine is 10%, and moistening is performed for 24h.

This application provides a stir-fried licorice formula granule, its preparation method, and quality control method. The method comprises: the stir-fried licorice formula granule is prepared from stir-fried licorice slices as raw material through water extraction, vacuum concentration, drying, and granulation. Each gram of formula granule is equivalent to 1.7-2.1 grams of stir-fried licorice slices, wherein the glycyrrhizin content is not less than 1.0%, and the glycyrrhizic acid content is not less than 2.1%. This invention also establishes a comprehensive quality control method for the formula granules. High-performance liquid chromatography (HPLC) is used to determine the content of key components, combined with chemical fingerprinting to evaluate overall chemical consistency, and physical fingerprinting is introduced to monitor the physical stability of the granules, forming a three-in-one quality control system of "component-chemistry-physics". This invention fills the technological gap in stir-fried licorice formula granules and provides a complete solution for their standardized production and safe and effective application.

The invention discloses a determination method of an Ajowan fruit fingerprint spectrum and the fingerprint spectrum thereof, and relates to the technical field of fingerprint spectrums of medicinal materials. The method comprises the following steps: taking rutin, caffeic acid, rosmarinic acid and p-coumaric acid as reference substances, dissolving the reference substances in a solvent, and fixing the volume to obtain a reference substance solution; the method comprises the following steps: taking different batches of ajowan fruit medicinal materials as test products, adding a solvent into the test products, extracting, filtering, and taking subsequent filtrate to obtain a test product solution; respectively injecting the reference substance solution and the test solution into a liquid chromatograph by utilizing a high performance liquid chromatography, recording a chromatogram, and constructing the fingerprint spectrum of the Ajowan fruit according to the chromatogram; and determining the component content of the test solution through an external standard method according to the chromatogram. According to the method, the quality of the ajowan fruit medicinal material is controlled by adopting a method of combining chemical component content determination and a fingerprint spectrum, so that the effective and comprehensive control on the ajowan fruit medicinal material is realized, and a basis is provided for the quality control standard of the ajowan fruit medicinal material.

The invention discloses a user side split-phase reactive power compensation control method and system based on HPLC communication, and relates to the field related to reactive power compensation control, and the method comprises the steps: obtaining a compensation monitoring point position, and arranging a reactive power compensation controller, a split-phase capacitor group and a common compensation capacitor group at the monitoring point position; current and voltage sampling is carried out on the monitoring point positions; performing data time sequence processing and power analysis on the current and voltage monitoring data sequence to obtain a three-phase power analysis index; the data is transmitted to the concentrator through the HPLC communication module for compensation constraint analysis; transmitting the three-phase power analysis index to a reactive power compensation controller, and performing reactive power compensation action instruction analysis by combining reactive power compensation control constraint and a reactive power compensation switching strategy; and reactive compensation control is carried out on the split-phase capacitor bank and the common compensation capacitor bank. The technical problem that reactive compensation is not accurate in existing split-phase reactive compensation control is solved, and the technical effects that split-phase reactive compensation of the user side is accurately controlled, and the power supply quality of a low-voltage transformer area is improved are achieved.

The invention provides a preparation method of clindamycin hydrochloride alcoholate. The preparation method comprises the following steps: firstly, preparing a chlorination reagent from an oily solvent, DMF (Dimethyl Formamide), a chlorination solvent and an antioxidant; carrying out temperature-controlled chlorination reaction with lincomycin hydrochloride to obtain a chlorination reaction solution; adjusting the pH value of the reaction solution to 10-11, controlling the temperature to react, taking an oil phase after the reaction is finished, concentrating under reduced pressure, diluting with water, and adjusting the pH value to 3-5 to obtain a column loading solution; the preparation method comprises the following steps: dissolving clindamycin hydrochloride in water, adsorbing by using resin, eluting impurities by using dilute alkaline water, analyzing by using ethanol and deionized water in sequence, collecting an analysis solution, concentrating under reduced pressure, adding ethanol, heating for dissolving, adjusting the pH value to 1-1.5, cooling for crystallizing, and drying to obtain the clindamycin hydrochloride alcoholate. According to the preparation method disclosed by the invention, the clindamycin alcoholate with high yield and high purity is prepared by pertinently designing processes such as reaction conditions, a specific oily reagent, temperature control conditions, column adsorption, dilute alkaline water impurity elution, ethanol desorption and the like, only one-time crystallization is needed, repeated refining is not needed, the HPLC (High Performance Liquid Chromatography) purity is up to 99.5% or above, and the clindamycin alcoholate is suitable for industrial production. And 0.14% or less of 2, 7-epiclindamycin.

The invention belongs to the technical field of natural pharmaceutical chemistry, and discloses a flavonoid compound in lomatogonium radiatum as well as a preparation method and application thereof.The flavonoid compound is separated from lomatogonium radiatum of a gentiaceae lomatogonium plant, the medicinal material of lomatogonium radiatum is extracted and concentrated, and the flavonoid compound is obtained. And sequentially carrying out macroporous adsorption resin separation, gel column chromatography separation, medium-low pressure ODS column chromatography separation and semi-preparative high performance liquid chromatography separation. Pharmacological studies show that the compound provided by the invention can effectively inhibit the levels of COX2, IL-6 and TNF-alpha in mouse macrophages RAW246.7 caused by lipopolysaccharide (LPS), which indicates that the compound has obvious anti-inflammatory activity and can be used for preparing anti-inflammatory drugs.

The invention relates to a detection method of a revivacin inhalation solution, and belongs to the technical field of medicine detection. According to the detection method provided by the invention, a revivin inhalation solution sample is analyzed by adopting high performance liquid chromatography to obtain the content of impurities in the revivin inhalation solution; the high performance liquid chromatography uses a phosphate buffer as a mobile phase A and acetonitrile as a mobile phase B, and the elution conditions are as follows: 0-5 min, the volume ratio of the mobile phase A to the mobile phase B is gradually changed from 95: 5 to 80: 20; the volume ratio of the mobile phase A to the mobile phase B is gradually changed from 80: 20 to 20: 80 in 5-30 min; the volume ratio of the mobile phase A to the mobile phase B is 95: 5. The method provided by the invention can realize accurate detection of five different impurities in the revienacin inhalation solution, and is beneficial to actual production quality control and improvement of drug safety.

This invention discloses an analytical method for detecting the content of 2,5-dihydroxybenzenesulfonic acid-1-hydroxypropyl ester in pharmaceutical creams using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). The detection steps are as follows: A certain amount of pharmaceutical cream is transferred to a volumetric flask, a certain amount of methanol is added, and the mixture is heated to disperse it evenly. After cooling to room temperature, the mixture is brought to a final volume, shaken, and centrifuged. The supernatant is then used for detection, and the results are calculated using the external standard method. The detection ions used in this method are 247.1>189.2 and 247.1>139.9. This method is rapid, simple, accurate, reliable, and highly sensitive, and can be used to detect the content of 2,5-dihydroxybenzenesulfonic acid-1-hydroxypropyl ester in creams.

The application discloses a dandelion medicinal material effective component rapid determination method based on color chroma values, and relates to the technical field of traditional Chinese medicinal material quality detection. * , a * , b * Chroma values of dandelion medicinal material powder are collected, and the contents of chicoric acid, monocaffeoyl tartaric acid and chlorogenic acid are determined by using high performance liquid chromatography as true values; subsequently, a neural network prediction model with chroma values as input and effective component contents as output is constructed and trained, preferably a single hidden layer structure; finally, the model is used for fast and nondestructive content prediction of unknown samples; compared with a traditional multiple linear regression model, the neural network model used in the application has significantly higher prediction accuracy, and the test set R² is all more than 0.86; the application realizes fast, nondestructive, low-cost and high-precision detection of dandelion medicinal material effective components, and is suitable for multiple scenes such as raw material quality control, market supervision, optimal harvest period judgment and fast pricing grading.

The invention relates to the technical field of power distribution network area topology identification, in particular to a combined topology identification method and system based on HPLC (High Performance Liquid Chromatography) and characteristic current injection, comprising the following steps: a control node broadcasts and sends a central beacon signal carrying a characteristic current sending instruction to a broadband power line carrier communication network; the characteristic current sending node sends a corresponding characteristic current signal by taking the initial position of a CSMA time slot area of a beacon period in which a central beacon signal is located as the initial time of sending the characteristic current signal, and the characteristic current identification node detects whether the characteristic current signal exists in a characteristic current signal detection window of the beacon period in which the central beacon signal is located; a detection result is reported to the control node, and the control node obtains a topology identification result of the power distribution area according to the detection result; according to the method, the beacon signaling and the whole network broadcasting system of the existing HPLC communication network are reused, so that the topology identification efficiency of the power distribution area is comprehensively improved, and the reliability and the accuracy of identification are enhanced.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to stasis-removing and pain-relieving pills and a quality detection method thereof. The stasis-removing and pain-relieving pill is prepared by taking radix paeoniae alba, rhizoma corydalis, radix paeoniae rubra, angelica sinensis, ligusticum wallichii, rhizoma cyperi, rhizoma zingiberis, fennel and astragalus membranaceus as raw materials and taking 10% starch slurry as an adhesive. The quality detection method of the stasis-removing and pain-relieving pill comprises general inspection, microscopic identification and thin-layer chromatography identification. And high performance liquid chromatography content determination. According to the present invention, by researching the preparation process and the quality standard of the stasis removing and pain relieving pill, the new thought and the new method are provided for the development of the traditional Chinese medicine preparation, the excellent choice is hopefully provided for the common gynecologic symptoms such as irregular menstruation, dysmenorrhea, postpartum blood stasis abdominal pain and the like, and the pain caused by the illness is relieved.

The application discloses a kind of phosphoric acid group modified β-cyclodextrin-based polymer film microextraction coating material and its application.First, a kind of phosphoric acid group β-CD porous crosslinked polymer (DBP-β-BNCD) and its preparation method are provided.The DBP-β-BNCD has the advantages of large specific surface area, narrow pore size distribution, large pore volume, good thermal stability, etc., which is beneficial to the accessibility of adsorption sites.When adsorbing hydrophilic pollutants antibiotics, various adsorption mechanisms such as polarity interaction, hydrogen bond interaction and π-π interaction are introduced.Further, by using DBP-β-BNCD as a thin film solid phase microextraction coating material, a method combining TFME with high performance liquid chromatography-tandem mass spectrometry (HPLC-QqQ-MS) is established, and the results show that the coating material has great potential for the extraction of hydrophilic organic pollutants in the environment.

The invention discloses a method for extracting active ingredients of a ligusticum wallichii-safflower medicinal pair by using a natural eutectic solvent and a process optimization method. The method comprises the following steps: 1) treating a sample; (2) preparing a natural eutectic solvent; 3) weighing mixed powder of ligusticum wallichii powder and safflower powder, adding the mixed powder into the L-proline-lactic acid natural eutectic solvent, mixing and extracting four active components; and (4) determining the four active ingredients by using high performance liquid chromatography and calculating the total extraction rate. According to the method, a natural eutectic solvent is used as an extraction solvent, four components in ligusticum wallichii and safflower are extracted, the extraction process is optimized by using a response surface method (RSM) and an ant colony-reverse neural network (ACO-BPNN), the ACO-BPNN has higher R2 and predictive value than the RSM, and the ACO-BPNN can better optimize the extraction process of various different polar compounds in plant drug pairs.

The application provides a method for detecting impurities in leboregen starting material LBS5, and belongs to the field of pharmaceutical analysis chemistry. S ,5 R )‑1‑(3‑fluorophenyl)‑3‑oxabicyclo[3.1.0]hexan‑2‑one. The method adopts high performance liquid chromatography, can simultaneously and accurately quantify one degradation impurity and four process impurities in LBS5, and has strong specificity, high sensitivity, good accuracy, good reproducibility and stable and reliable method. The method can realize the control of the impurities, ensures the safety of LBS5 as a pharmaceutical intermediate, further ensures the safety of a preparation of LBS5, reduces the risk of drug use, simultaneously reduces the difficulty of subsequent synthesis steps, realizes rapid, economic and environmental protection production. The method is suitable for laboratory research and development, and is also suitable for quality control in commercial production.

The application discloses a kind of compound Xianghua granules fingerprint's construction method, comprising the following steps: preparation of reference solution: p-coumaric acid, ferulic acid standard in methanol is dissolved;Preparation of test solution: different batches of compound Xianghua granules are dissolved in ultrapure water respectively, and post-processing is carried out;Respectively inject high performance liquid chromatography and detect, obtain the fingerprint of reference solution and the fingerprint of each batch sample;Using traditional Chinese medicine fingerprint similarity evaluation system analyzes the fingerprint of each batch sample, generates the control fingerprint of compound Xianghua granules.The method provided by the application can better reflect the multiple index component information of compound Xianghua granules.The fingerprint characteristic peak baseline of the application is smooth, and the sensitivity and separation degree are better, while the operation is quick and reliable, the cost is lower, can effectively, stably and quickly comprehensively monitor product quality, ensure the stability and quality controllability of product.

The invention belongs to the technical field of medicines, and particularly relates to an infantile cough relieving syrup detection method which comprises the following steps: identifying radix peucedani, platycodon grandiflorum, pericarpium citri reticulatae and cortex mori radicis in infantile cough relieving syrup by adopting a thin layer chromatography; and simultaneously detecting the fructus arctii and the radix scutellariae by adopting a high performance liquid chromatography. The detection method for the infantile cough relieving syrup has the advantages of being rapid in analysis, easy to operate, high in accuracy, wide in adaptability and the like, and establishment and overall evaluation of the quality standard of the infantile cough relieving syrup are facilitated.

The invention discloses a method for directly preparing a racemic oxidation product from a fennerenone enantiomer through photocatalysis, and belongs to the technical field of organic synthesis.The method comprises the steps that an R-type fennerenone isomer is taken to be placed in a reaction bottle, a photosensitizer containing iridium or ruthenium is added, and a solvent is added at the speed of 0.1 M in the air atmosphere; the method comprises the following steps: dissolving 4-(4-cyano-2-methoxyphenyl)-5-ethoxy-2, 8-dimethyl-1, 6-naphthyridine-3-formamide in a solvent, stirring, transferring a mixture into a photoreactor of a blue light LED (Light Emitting Diode) with the wavelength of 455-465 nm, stirring, transferring a mixed solution after the reaction is finished into a round-bottom flask, purifying a volatile solvent to obtain a product 4-(4-cyano-2-methoxyphenyl)-5-ethoxy-2, 8-dimethyl-1, 6-naphthyridine-3-formamide, and carrying out chiral HPLC (High Performance Liquid Chromatography) analysis on the product. High temperature, high pressure or a strong oxidant is not needed, and reaction conditions are mild; oxygen in air is oxidized at room temperature, so that energy consumption and pollutant generation are reduced; compared with the traditional oxidation condition, the method does not need to undergo the process of oxidation first and then high-temperature induced racemization, and the racemization product can be directly obtained.

The invention belongs to the technical field of biological medicines, and particularly relates to a dissociation liquid applied to an aluminum adjuvant adsorption influenza vaccine and a method for measuring the antigen content. The dissociation liquid disclosed by the invention is prepared from a potassium phosphate salt solution, diethanol amine, TritonX-100, tween-20, dodecyl dimethyl betaine and water. The dissociation liquid is excellent in dissociation efficiency, the dissociation recovery rate of the dissociation liquid to H1N1, H3N2 and BV type influenza vaccine hemagglutinin reaches 106%-110%, the antigen activity can be effectively protected, operation is easy and convenient, adaptability is wide, a dissociated sample can be directly used for mainstream detection methods such as a one-way immunodiffusion test, an enzyme-linked immunosorbent assay and a high performance liquid chromatography method, and the dissociation liquid has good application prospects. The precise quantification of the antigen content in the aluminum adjuvant adsorption influenza vaccine is realized, and a reliable technical support is provided for vaccine quality control.

The invention aims to establish a synergistic catalytic system in which catalysts cannot coexist. The amide chiral catalyst is prepared through a series of synthesis. The hollow mesoporous COF-carboxylic acid / chiral amide multi-catalyst is prepared by a hydrothermal method. The morphology and the structure of the material are characterized by using a scanning electron microscope, a transmission electron microscope, an X-ray diffractometer, a specific surface instrument, an infrared spectrometer and the like, an asymmetric tandem-addition reaction is catalyzed to construct a chiral acetal compound, high performance liquid chromatography analysis is combined, and multiple catalysts are continuously recycled. Results show that the hollow mesoporous COF-carboxylic acid / chiral amide multi-catalyst not only has good morphology, excellent specific surface area and high porosity, but also enables contradictory catalysts to coexist for synergistic catalysis. The catalyst can be used for rapidly catalyzing an asymmetric tandem-addition reaction, high selectivity of ee = 99% and high yield of 98% are achieved, and the catalyst can be repeatedly used for 10 times. The method is green, environment-friendly, low in cost and suitable for concerted catalysis of catalysts which cannot coexist.

The present application relates to a kind of saponins HZ extracted from shi medicine twelve hours and its preparation method and application.Twelve hours saponins HZ is prepared using the following method: with shi medicine twelve hours as raw material, using impregnation extraction, extraction, microporous resin MCI column chromatography separation to obtain extract Fr.B4, extract Fr.B4 is purified by Sephadex LH-20, obtain FrB4-1~FrB4-6 6 components, component FrB4-4 is separated by semi-preparative high performance liquid chromatography (CH3CN:H2O=30:70, 3.0mL / min) to obtain twelve hours saponins HZ.Twelve hours saponins HZ has significant effect in the treatment of ulcerative colitis, and can be further used for developing safe, effective, controllable traditional Chinese medicine anti-inflammatory, ulcerative colitis new drug.

The application belongs to the field of analytical chemistry detection, and particularly relates to a method for detecting radiochemical purity of an actinium 225 Ac] conjugate. The method adopts high performance liquid chromatography combined with a gamma counter, a mobile phase includes mobile phase A and mobile phase B, the mobile phase A is a mixed solution of buffer salt and cationic pair reagent, and the mobile phase B is acetonitrile. 225 The detection method can accurately detect the radiochemical purity of the actinium 225 Ac] conjugate, is stable and reliable, has high sensitivity, and is of great significance for quality control and research of the actinium [Ac] conjugate product.