The present invention relates to
phytase which is obtained through the
gene engineering reconstruction, and provides the
phytase which is reconstructed through the rite-
directed mutagenesis, the
phytase is produced by manufacturing a plurality of amino acids in the phytase which is derived from
aspergillus niger and has the serial number of the
amino acid of EQ ID No.1 to be replaced, and the replacement of the
amino acid comprises a 23rd replacement, a 53rd replacement, a 136th replacement, a 137th replacement, and a 195th replacement. The present invention discloses the optimal and reconstructive phytase and the corresponding
DNA sequence, and also discloses the production method and the application of the phytase. The phytase of the present invention is better in the stand up quality of the temperature, and the
pH range which is most suitable for the
enzyme activity is also improved; further, a
mutant gene is highly and effectively expressed through a
bioreactor, to lead the phytase after the reconstruction and the rite-
directed mutagenesis to be highly and effectively expressed, thereby finally achieving the requirement of industrialized production.