16 results about "Genetic structure" patented technology

The invention relates to the technical field of bioinformatics, in particular to a goat selective breeding method and system based on a genetic algorithm, and the method comprises the following steps: based on goat population genetic initialization data, extracting a character characteristic index and a genetic relationship parameter of each pairing combination in a population, analyzing genetic diversity, character matching degree and genetic relationship, and calculating the genetic diversity, character matching degree and genetic relationship; and screening matched individuals and pairing hereditary characteristics, and rearranging and recombining to obtain primary pairing data of goat breeding. According to the method, genetic diversity and character matching degree are analyzed, genetic diversity and excellent character inheritance are balanced, genetic resource utilization efficiency is improved, local optimal combinations are accurately screened, genetic drift risks are reduced, character stability is enhanced, a two-point crossing rule and global character dynamic analysis are combined, and global breeding layout is optimized; according to the method, short-term improvement and long-term stability are both considered, the genetic structure is optimized through Pareto rule screening and global state updating, and the sustainability and accuracy of a breeding scheme are improved.

The invention belongs to the technical field of molecular biology, and particularly relates to a single-copy nuclear gene marker of elaeagnus mollis, a primer pair and application of the single-copy nuclear gene marker. The elaeagnus mollis single-copy nuclear gene marker comprises any one or more of elaeagnus mollis single-copy nuclear gene markers 1, 2, 3, 4, 5, 6 and 7; the nucleotide sequences of the single-copy nuclear gene marker of the elaeagnus mollis are sequentially as shown in SEQ ID NO.1 to SEQ ID NO.7. The invention further discloses a preparation method of the single-copy nuclear gene marker. The single-copy nuclear gene marker primer pair of elaeagnus mollis provided by the invention has very high universality, amplification sequencing can be performed in selected elaeagnus mollis individuals of different provenances, a genetic diversity index analysis result is good, and all individuals of different provenances can be obviously distinguished according to a genetic structure analysis result; the method is suitable for genetic diversity analysis of large-scale germplasm resources of elaeagnus mollis.

The invention provides a cyclobalanopsis rupestris EST-SSR (expressed sequence tag-simple sequence repeat) marker primer, the primer comprises seven primer pairs which are P2, P11, P24, P25, P29, P32 and P34 in sequence, and the sequences are shown as SEQ ID NO: 1-14, the invention further provides a preparation method of the EST-SSR marker primer, and the preparation method comprises the following steps: S1, selecting a cyclobalanopsis rupestris sample, sampling, drying and storing; s2, carrying out DNA extraction and purity detection on the sample, and then storing all extracted sample DNAs for later use; and S3, selecting a DNA sample to establish a PCR amplification system, then carrying out PCR amplification to obtain pre-selected primers, and then screening the pre-selected primers to obtain the cyclobalanopsis gilgiana EST-SSR labeled primer. The invention also comprises the application of the EST-SSR labeled primer in population genetic diversity, population genetic structure analysis, population genetic differentiation analysis, genetic distance and clustering analysis of the EST-SSR labeled primer. The cyclobalanopsis gilgii leaf EST-SSR marker primer fills up the technical vacancy in the field, and provides a research basis for cyclobalanopsis gilgii population protection and germplasm resource preservation and utilization.

The present invention relates to the field of molecular biology, and in particular to polymorphic EST-SSR molecular marker primers for longitudinal pine and their applications. The nucleotide sequence of the primer set is shown in SEQ ID NO.1-SEQ ID NO.40. The primer set provided by the present invention can specifically amplify the EST-SSR molecular markers, and has a high degree of polymorphism, and can be applied to research fields such as longitudinal pine genetic diversity and population genetic structure. Moreover, the EST-SS molecular markers amplified by the EST-SSR primer set of the present invention have the advantages of abundant quantity, high polymorphism and good stability. At the same time, the amplification experiment of the EST-SSR primer set in the present invention is simple to operate, and the bands are clear and easy to distinguish, which is suitable for genetic diversity and genetic structure analysis of longitudinal pine.

According to the myricaria laxiflora population genetic analysis method based on simplified genome sequencing, the myricaria laxiflora genetic diversity and population genetic structure analysis is carried out through SNP molecular markers, high repeatability and operability are achieved, and the myricaria laxiflora population genetic analysis method has great significance in myricaria laxiflora genetic resource evaluation and utilization; the method also has reference significance for evaluation work of genetic resources of other endangered wild plants; according to the method, the myricaria laxiflora population with high genetic diversity can be screened out according to the obtained population genetic variation data, and the myricaria laxiflora population is used as a prioritized myricaria laxiflora population, so that the method has guiding significance on in-situ protection work of myricaria laxiflora and is beneficial to formulating a myricaria laxiflora species protection plan and a genetic management strategy.

The invention belongs to the technical field of biomolecular markers, and particularly relates to a microsatellite marker for evaluating genetic diversity of metapenaeus ensis as well as a primer pair combination and application of the microsatellite marker. By extracting genomes from different metapenaeus ensis populations, developing 10 microsatellite markers through transcriptome data and designing corresponding primer pair combinations, the microsatellite markers provided by the invention have the characteristics of stable PCR amplification result, high polymorphism and the like, can be used for researching diversity, population genetic structures, evolution, genetic relationships and the like of metapenaeus ensis, and have a wide application prospect. The method is used for evaluating genetic structures and polymorphisms of metapenaeus ensis in different areas and different populations, provides a theoretical basis for parent shrimp breeding work and resource protection of the metapenaeus ensis, and has a good application value.

The present disclosure provides a combination of single sequence repeat (SSR) molecular marker for Procapra przewalskii, a primer combination, a kit, and use, and relates to the technical field of molecular biology. In the present disclosure, design of SSR primers and screening of SSR loci are conducted based on a whole-genome sequencing data of Procapra przewalskii. 26 pairs of obtained SSR primers can stably amplify a target product, and are highly polymorphic, and can be used for population genetic diversity detection, population genetic structure analysis, evolution, and kinship research of the Procapra przewalskii. A combination of polymorphic SSR molecular marker of Procapra przewalskii selected can be used for individual identification of the Procapra przewalskii with a high accuracy. The experimental results show that the combination of SSR molecular marker of Procapra przewalskii can meet the needs of individual identification.

This invention belongs to the field of Taihang flower research. It discloses 15 pairs of specific SSR primers for Taihang flower, a national second-class protected plant and endangered species, and their applications in the analysis of genetic diversity and genetic structure characteristics of the endangered species Taihang flower population. This can reveal the phylogenetic and evolutionary relationship of Taihang flower populations and has important theoretical significance for the research and protection of the endangered species Taihang flower.

The invention discloses an efficient and accurate wheat parent matching method and system based on the distance between a core germplasm and a genome, and belongs to the technical field of cross breeding. The matching method comprises the following steps: acquiring whole genome SNP genotype data of a breeding population, constructing a core germplasm of which the sample size is smaller than that of an original population but the original genetic diversity is greater than or equal to 95%, analyzing the population genetic structure of the core germplasm, calculating the genome distance between individuals of the core germplasm, and selecting the whole genome SNP genotype data of the breeding population. And screening Top N combinations with maximum and minimum genome distances by taking parents from different genetic subgroups as a primary condition and taking an interval from the top quartile to the maximum value of the genome distance as a primary screening range, and outputting a recommended hybrid combination list. According to the method, quantitative indexes of the genetic population structure and the genome distance are innovatively coupled through an algorithm model, double screening of subpopulation complementation and distance optimization is achieved, decision logic is more scientific, and prediction accuracy is higher.

The present invention relates to a genetic marker for individual identification of white pheasants and an application thereof, and relates to the field of biotechnology. The genetic marker comprises at least 20 of the SNP sites 1-86. The genetic marker is used to perform individual identification of white pheasants, and the individual identification result is accurate and reliable. Based on the individual identification result, kinship, population genetic diversity, and population genetic structure analysis can also be performed, meeting the protection research needs of the white pheasant species.

The application belongs to the technical field of molecular biology DNA marker, and specifically discloses a microsatellite marker site of Anemone coronaria, which comprises at least one of ZH3008, ZH7380, ZH13016, ZH17408, ZH18659, ZH18997, ZH19490, ZH19729 and ZH21570. The application provides a primer group for amplifying the microsatellite marker site of Anemone coronaria, and application of the microsatellite marker site of Anemone coronaria in population genetic structure analysis, paternity identification and / or molecular marker assisted breeding. The microsatellite marker site of Anemone coronaria and the primer group for amplifying the microsatellite marker site provided by the application can be applied to research in the fields of population genetic structure analysis, paternity identification and molecular marker assisted breeding of Anemone coronaria, and have good repeatability, are reliable and effective molecular markers, and are important tools for studying genetic diversity and connectivity of Anemone coronaria.

The invention discloses SSR molecular marker development and population genetic diversity analysis application based on polygonatum sibiricum leaf transcriptome data, and belongs to the technical field of biology. On the basis of polygonatum sibiricum tender leaf transcriptome sequencing, screening and batch screening are performed on a large amount of tender leaf transcriptome data, a unigene sequence rich in perfect SSR sites is obtained, a polymorphic SSR molecular marker is developed, and the application of the polymorphic SSR molecular marker to the detection of polygonatum sibiricum tender leaf transcriptome sequencing is realized. The problems that at present, the number of SSR molecular markers of the polygonatum sibiricum genome is small, molecular genetics research and application are limited, and molecular marker-assisted genetic breeding is obstructed are solved. The effectiveness of the developed SSR marker is verified by analyzing the genetic diversity of the polygonatum sibiricum population, and a foundation is laid for research on genetic diversity analysis, genetic structure analysis, genetic map construction, target gene cloning, molecular marker-assisted breeding and the like of polygonatum sibiricum and other species of polygonatum sibiricum.

The invention relates to a variety-specific SNP (Single Nucleotide Polymorphism) site screening method and application thereof, and belongs to the technical field of variety identification. According to the variety specific SNP site screening method and the variety identification strategy suitable for the multiple species, by combining specific SNP site screening and population genetic structure model analysis, variety specific sites can be rapidly and accurately screened, variety identification can be achieved, the accuracy and efficiency of variety identification are greatly improved, and the method is suitable for large-scale popularization and application. Therefore, technical support is provided for germplasm resource protection, breeding innovation and high-quality development of seed industry.

The invention relates to an SSR primer group for genetic diversity analysis and genetic relationship identification of larva cavernae as well as an acquisition method and application of the SSR primer group, and belongs to the technical field of DNA molecular markers. The SSR molecular marker primer composition for larva cavernae provided by the invention comprises at least one of 24 pairs of primers, the nucleotide sequences of the 24 pairs of primers are as shown in SEQ ID No.1-SEQ ID No.48, and the nucleotide sequences of the 24 pairs of primers are as shown in SEQ ID No.2-SEQ ID No.3. The SSR core primer group provided by the invention has the advantages of uniform distribution, high polymorphism, good amplification repeatability, clear banding pattern and easiness in interpretation in the genome of the larva cavernae, is suitable for detection of a capillary fluorescence detection platform, and can be applied to identification of the larva cavernae, genetic diversity analysis, population genetic structure, genetic relationship identification and the like.

The invention discloses an ostrea rivularis EST sequence source microsatellite specific primer group and application thereof, and belongs to the technical field of molecular genetics, and nucleotide sequences of primers are shown as SEQ ID NO.1-24. The invention also provides a kit containing the primer group. The invention further provides the primer group which can be used for analyzing the population genetic diversity, population genetic structure and genetic relationship identification of the ostrea rivularis and performing genetic evaluation on different generations of the ostrea rivularis.

The invention belongs to the technical field of molecular markers, and particularly relates to microsatellite molecular markers universal for polymorphism of marmot and marmot glauca and application of the microsatellite molecular markers. The invention discloses a microsatellite molecular marker universal for polymorphism of marmot and marmot glauca. The microsatellite molecular marker comprises at least one of eight STR (short tandem repeat) loci (XJM011, XJM015, XJM022, XJM041, XJM064, XJM068, XJM071 and XJM092). Moreover, corresponding identification primer pairs are respectively provided aiming at the eight STR loci, the microsatellite loci can be subjected to specific amplification, and high polymorphism is achieved; the method can be applied to the research fields of Xinjiang Parmir plateau marmot and marmot marmot, sibling species and sibling species genetic diversity, population genetic structure, pedigree geography, evolution and genetic relationship and the like, and has excellent application prospects.