10 results about "Genetic structure" patented technology

The invention belongs to the technical field of biomolecular markers, and particularly relates to a microsatellite marker for evaluating genetic diversity of metapenaeus ensis as well as a primer pair combination and application of the microsatellite marker. By extracting genomes from different metapenaeus ensis populations, developing 10 microsatellite markers through transcriptome data and designing corresponding primer pair combinations, the microsatellite markers provided by the invention have the characteristics of stable PCR amplification result, high polymorphism and the like, can be used for researching diversity, population genetic structures, evolution, genetic relationships and the like of metapenaeus ensis, and have a wide application prospect. The method is used for evaluating genetic structures and polymorphisms of metapenaeus ensis in different areas and different populations, provides a theoretical basis for parent shrimp breeding work and resource protection of the metapenaeus ensis, and has a good application value.

The present disclosure provides a combination of single sequence repeat (SSR) molecular marker for Procapra przewalskii, a primer combination, a kit, and use, and relates to the technical field of molecular biology. In the present disclosure, design of SSR primers and screening of SSR loci are conducted based on a whole-genome sequencing data of Procapra przewalskii. 26 pairs of obtained SSR primers can stably amplify a target product, and are highly polymorphic, and can be used for population genetic diversity detection, population genetic structure analysis, evolution, and kinship research of the Procapra przewalskii. A combination of polymorphic SSR molecular marker of Procapra przewalskii selected can be used for individual identification of the Procapra przewalskii with a high accuracy. The experimental results show that the combination of SSR molecular marker of Procapra przewalskii can meet the needs of individual identification.

This invention belongs to the field of Taihang flower research. It discloses 15 pairs of specific SSR primers for Taihang flower, a national second-class protected plant and endangered species, and their applications in the analysis of genetic diversity and genetic structure characteristics of the endangered species Taihang flower population. This can reveal the phylogenetic and evolutionary relationship of Taihang flower populations and has important theoretical significance for the research and protection of the endangered species Taihang flower.

The invention discloses an efficient and accurate wheat parent matching method and system based on the distance between a core germplasm and a genome, and belongs to the technical field of cross breeding. The matching method comprises the following steps: acquiring whole genome SNP genotype data of a breeding population, constructing a core germplasm of which the sample size is smaller than that of an original population but the original genetic diversity is greater than or equal to 95%, analyzing the population genetic structure of the core germplasm, calculating the genome distance between individuals of the core germplasm, and selecting the whole genome SNP genotype data of the breeding population. And screening Top N combinations with maximum and minimum genome distances by taking parents from different genetic subgroups as a primary condition and taking an interval from the top quartile to the maximum value of the genome distance as a primary screening range, and outputting a recommended hybrid combination list. According to the method, quantitative indexes of the genetic population structure and the genome distance are innovatively coupled through an algorithm model, double screening of subpopulation complementation and distance optimization is achieved, decision logic is more scientific, and prediction accuracy is higher.

The application belongs to the technical field of molecular biology DNA marker, and specifically discloses a microsatellite marker site of Anemone coronaria, which comprises at least one of ZH3008, ZH7380, ZH13016, ZH17408, ZH18659, ZH18997, ZH19490, ZH19729 and ZH21570. The application provides a primer group for amplifying the microsatellite marker site of Anemone coronaria, and application of the microsatellite marker site of Anemone coronaria in population genetic structure analysis, paternity identification and / or molecular marker assisted breeding. The microsatellite marker site of Anemone coronaria and the primer group for amplifying the microsatellite marker site provided by the application can be applied to research in the fields of population genetic structure analysis, paternity identification and molecular marker assisted breeding of Anemone coronaria, and have good repeatability, are reliable and effective molecular markers, and are important tools for studying genetic diversity and connectivity of Anemone coronaria.

The invention discloses SSR molecular marker development and population genetic diversity analysis application based on polygonatum sibiricum leaf transcriptome data, and belongs to the technical field of biology. On the basis of polygonatum sibiricum tender leaf transcriptome sequencing, screening and batch screening are performed on a large amount of tender leaf transcriptome data, a unigene sequence rich in perfect SSR sites is obtained, a polymorphic SSR molecular marker is developed, and the application of the polymorphic SSR molecular marker to the detection of polygonatum sibiricum tender leaf transcriptome sequencing is realized. The problems that at present, the number of SSR molecular markers of the polygonatum sibiricum genome is small, molecular genetics research and application are limited, and molecular marker-assisted genetic breeding is obstructed are solved. The effectiveness of the developed SSR marker is verified by analyzing the genetic diversity of the polygonatum sibiricum population, and a foundation is laid for research on genetic diversity analysis, genetic structure analysis, genetic map construction, target gene cloning, molecular marker-assisted breeding and the like of polygonatum sibiricum and other species of polygonatum sibiricum.

The invention relates to a variety-specific SNP (Single Nucleotide Polymorphism) site screening method and application thereof, and belongs to the technical field of variety identification. According to the variety specific SNP site screening method and the variety identification strategy suitable for the multiple species, by combining specific SNP site screening and population genetic structure model analysis, variety specific sites can be rapidly and accurately screened, variety identification can be achieved, the accuracy and efficiency of variety identification are greatly improved, and the method is suitable for large-scale popularization and application. Therefore, technical support is provided for germplasm resource protection, breeding innovation and high-quality development of seed industry.

The invention relates to an SSR primer group for genetic diversity analysis and genetic relationship identification of larva cavernae as well as an acquisition method and application of the SSR primer group, and belongs to the technical field of DNA molecular markers. The SSR molecular marker primer composition for larva cavernae provided by the invention comprises at least one of 24 pairs of primers, the nucleotide sequences of the 24 pairs of primers are as shown in SEQ ID No.1-SEQ ID No.48, and the nucleotide sequences of the 24 pairs of primers are as shown in SEQ ID No.2-SEQ ID No.3. The SSR core primer group provided by the invention has the advantages of uniform distribution, high polymorphism, good amplification repeatability, clear banding pattern and easiness in interpretation in the genome of the larva cavernae, is suitable for detection of a capillary fluorescence detection platform, and can be applied to identification of the larva cavernae, genetic diversity analysis, population genetic structure, genetic relationship identification and the like.

The invention discloses an ostrea rivularis EST sequence source microsatellite specific primer group and application thereof, and belongs to the technical field of molecular genetics, and nucleotide sequences of primers are shown as SEQ ID NO.1-24. The invention also provides a kit containing the primer group. The invention further provides the primer group which can be used for analyzing the population genetic diversity, population genetic structure and genetic relationship identification of the ostrea rivularis and performing genetic evaluation on different generations of the ostrea rivularis.

The invention belongs to the technical field of molecular markers, and particularly relates to microsatellite molecular markers universal for polymorphism of marmot and marmot glauca and application of the microsatellite molecular markers. The invention discloses a microsatellite molecular marker universal for polymorphism of marmot and marmot glauca. The microsatellite molecular marker comprises at least one of eight STR (short tandem repeat) loci (XJM011, XJM015, XJM022, XJM041, XJM064, XJM068, XJM071 and XJM092). Moreover, corresponding identification primer pairs are respectively provided aiming at the eight STR loci, the microsatellite loci can be subjected to specific amplification, and high polymorphism is achieved; the method can be applied to the research fields of Xinjiang Parmir plateau marmot and marmot marmot, sibling species and sibling species genetic diversity, population genetic structure, pedigree geography, evolution and genetic relationship and the like, and has excellent application prospects.