131 results about "Paternity tests" patented technology

The self-adaptive test system for WIFI products of the present invention includes: a signal transmitting module, a signal processing module, a signal detection module and accompanying testing equipment; wherein: the signal transmitting module is used to send interference signals and blocking signals to the signal according to pre-configured working parameters A processing module; a signal processing module, which is used to attenuate the interference signal and the blocking signal according to the pre-acquired attenuation data, and then couple them to the communication link between the device under test and the device under test; it is also used to transmit the signal sent by the device under test The test signal is sent to the signal detection module and the accompanying test device after signal processing; the signal detection module is used to detect the test signal after signal processing; when the test signal is detected, it is determined that the self-adaptive test of the device under test is unqualified; When no test signal is detected, it is determined that the adaptive test of the device under test is qualified. The invention reduces the test cost, and each module of the test is easy to obtain and easy to operate.

The invention belongs to the technical field of biological detection, and in particular relates to a fluorescently-labeled insertion / deletion (InDel) genetic polymorphism locus composite amplification system and application thereof. The application comprises the following steps of: selecting 35 InDel loci, designing polymerase chain reaction (PCR) amplification primers of the 35 InDel loci, matching fluorescein labels of the PCR primers, compositely amplifying and detecting the 35 InDel loci, and the like. The system can analyze 35 InDel genetic polymorphism loci by the composite amplification. The 35 InDel loci are labeled by FAM, HEX, TAMRA and ROX fluorescein with four different colors respectively. The composite amplification system based on the invention can be made into a kit, serves as an InDel locus fluorescence composite amplification kit with Chinese characteristics, and is applied to the fields, such as triplet paternity test, doublet paternity test, grandparent and grandchild test, sibling test, individual recognition, disease diagnosis, anthropology and the like.

The invention relates to an SNPs detection method, and concretely relates to a method for detecting specifically amplified products through a one-step PCR reaction and a nucleic acid detection test paper strip in a same reaction system. The method comprises the following steps: nonspecifically amplifying fragments containing SNPs sites through first PCR temperature cycling; specifically amplifying bases containing the SNPs sites through AS-PCR (allele specific polymerase chain reaction); and detecting the specifically amplified products by the nucleic acid detection test paper strip. The method can also be used for detecting mononucleotide mutation. The method which has the characteristics of high sensitivity, strong specificity, simple operation, short time, and rapid and reliable detection result combines respective advantages of proteins and nucleic acids. The invention also relates to applications of a kit in the detection of known drug-resistant gene of pathogens, the mating selection of a donor and an acceptor in organ transplantation, and the discrimination of the identity of a criminal or paternity tests in forensic researches.

The invention discloses a method for a paternity test through utilizing a specific single nucleotide polymorphism (SNP) mark combination which comprises 60 SNP marks. The nucleotide sequences of the forward primers of the SNP marks are shown as SEQ ID NO.1-60; the nucleotide sequences of downstream primers are shown as SEQ ID NO.61-120; and the nucleotide sequences of single base extension primers are shown as SEQ ID NO.121-180. The method utilizes a flight mass spectrum method, the SNP mark combination is used for human paternity tests, and the method has the advantages of accurate result, quickness and convenience in method and high flux.

The invention relates to a high-throughput sequencing-based detection kit for SNP loci used for detecting 273 SNP loci (234 SNP loci are located on an autosome, 9 SNP loci are located on a Y chromosome and 30 SNP loci are located on an X chromosome). The detection kit comprises a primer composition formed by primer pairs of 273 SNP loci. The sequence of the primer composition is as shown in SEQ ID NO:1 to SEQ ID NO:546. The invention further provides a detection method using the kit, and an application of the kit in the field of judicial identification of triplet paternity test, diad paternity test, grandparent and grandchild test, siblings identification and individual recognition. Parallel sequencing is carried out on the SNP loci by adopting a high-throughput sequencing technology, and variation information of a flanking sequence can be obtained while locus information is read; sequence information of 384 samples on the 273 SNP loci can be obtained through single sequencing, so that the DNA sample capacity is reduced and the detection time is shortened; and fragment libraries are concentrated below 200bp and are suitable for forensic degradable materials.

The invention discloses an SSR fluorescence labeling primer for the paternity test of Chinese sturgeon and application. 10 pairs of fluorescence labeling microsatellite primers are successfully screened and synthesized and can be used for establishing a Chinese sturgeon paternity test technology. The invention also discloses a kit for the paternity test. Synthesized PCR products with different fluorescence colors are mixed, and then allelic genes are tested to carry out the paternity test analysis of the Chinese sturgeon. A identification method is easy and fast to operate and low in cost, has the characteristics of high efficiency, economy, easiness, convenience for operation and the like, can be popularized and applied to the germplasm identification, family management and fine breed breeding and can be used for evaluating the manual proliferative effect and releasing effect.

The invention provides a sextuple PCR (polymerase chain reaction) detection method of a portunus trituberculatus miers microsatellite marker. The detection method comprises the following steps of: designing and synthesizing a sextuple PCR primer; extracting genome DNA (deoxyribonucleic acid); performing sextuple PCR reaction; and detecting a PCR product, wherein the sextuple PCR reaction comprises a sextuple PCR reaction system and a sextuple PCR reaction procedure. The method builds the sextuple PCR reaction system which performs the portunus trituberculatus miers family and paternity test to simultaneously detect six microsatellite sites in the PCR reaction, so that efficiency is improved by about six times compared with the existing PCR method. The method has the characteristics of being high-efficiency, economical, simple, convenient and the like, thereby being capable of being popularized and applied in the portunus trituberculatus miers genetic diversity analysis, the genetic relationship test, the family management and the improved variety breeding.

The invention discloses microsatellite DNA markers for Trionyx sinensis, which is characterized in that an enriched library of microsatellites (CA)n, (AAC)n, (ACAG)n and (GATA)n from the Trionyx sinensis is constructed, and positive clones of microsatellite sequences are screened and sequenced to obtain 63 clones containing microsatellite repetitive sequences, thus determining 15 microsatellite markers with rich polymorphism, namely, PSW01, PSW02, PSW03, PSW04, PSW05, PSW06, PSW07, PSW08, PSW09, PSW10, PSW11, PSW12, PSW13, PSW14 and PSW15. The microsatellite DNA markers for the Trionyx sinensis of the invention can be applied to researching genetic diversity and paternity test for the Trionyx sinensis in different geographical populations, have the advantage of good repeatability, and are reliable and effective molecular markers.

The invention supplies a fluorescence labeling verification system by compounding and expanding 14 short series repeating sequence locus to take personal identification and paternity test. The specific compounding method controls the expanding product in the range of 400 basic groups, and only labeling three fluorescence of the compounding expanding of the 14 locus would be realized. The invention takes a deep research to the gene characteristic of the 14 locus and the allele in five minorities in China, and supplies the compounding expanding verification system. The agent box suited for Chinese throng is researched.

The invention discloses a method for multiplex amplification of a pig microsatellite marker and a special primer thereof. A primer composition provided by the invention consists of a primer pair 1, a primer pair 2, a primer pair 3, a primer pair 4, a primer pair 5, a primer pair 6, a primer pair 7, a primer pair 8, a primer pair 9 and a primer pair 10. Experiments prove that the data obtained by performing multiplex amplification and single PCR (Polymerase Chain Reaction) amplification by selecting a specific primer pair at a specific proportion are completely consistent, so that the accuracy and the feasibility of decuplet PCR provided by the invention are verified. The multiplex amplification method provided by the invention has great significance to paternity test, breeding and production of pigs.

The invention discloses microsatellite marker primers used for penaeus monodon microsatellite family identification. The microsatellite marker primers include six primer pairs in total, and the six primer pairs are PM-38, PM-69, PM-92, PM-84, PM-102 and PM-114 respectively. The invention further discloses an identification method for the penaeus monodon microsatellite family and application of the microsatellite marker primers to penaeus monodon microsatellite family identification. By the adoption of the microsatellite marker primers, a paternity test platform is set up on penaeus monodon with polymorphic microsatellite markers marked by fluorescence for the first time, and the identification accuracy reaches 99%; different families and sources of the penaeus monodon can be effectively and fast identified, and a basis is provided for breeding, reproduction matching and enhancement and releasing assessment of the penaeus monodon.

The invention belongs to the technical field of fish breeding, relates to the technical field of molecular markers, and particularly relates to a method for accurately identifying a megalobrama amblycephala polyculture family by utilizing a microsatellite marker. The method comprises the following steps: (1) adopting an artificial propagation method for breeding a megalobrama amblycephala family,and breeding 54 family progenies in a same pond for polyculture; (2) adopting a fluorescent marker primer and a nested PCR amplification method for analyzing genotypes of a parent at 12 microsatellite sites, and screening out effective microsatellites suitable for family identification; (3) analyzing the genotypes of the family progenies in polyculture at the effective microsatellite sites; and (4) identifying parent sources of all the progenies. In the invention, a paternity test method is established for megalobrama amblycephala for the first time, with the identification accuracy rate up to 98.4%. Through the family screening and identification, the phenomenon of inbreeding existing in the breeding of megalobrama amblycephala in groups can be reduced so as to be more advantageous to the realization of family breeding, speed up the process of breeding, and improve the breeding effect.

The invention relates to a multiple PCR (polymerase chain reaction) primer combination used for a human paternity test. Eighty SNP (single nucleotide polymorphellosm) genetic markers can be detected by virtue of the primer combination. Nucleotide sequences of primers at middle and upper reaches in the primer combination are respectively shown in SEQ ID NO.1-80; nucleotide sequences of primers at a lower reach in the primer combination are respectively shown in SEQ ID NO.81-160; and nucleotide sequences of single-base extension primers are sequentially shown in SEQ ID NO.161-240. According to the multiple PCR primer combination, the human paternity test is carried out by preferably utilizing a flight time spectral method, a result is accurate, a method is rapid and simple, and flux is high, so that the multiple PCR primer combination has a good application prospect.

The invention discloses a kit used for a paternity test of giant pandas. 68 allelic genes of 11 STR gene loci, obtained by microsatellite study, of the giant pandas are taken as typing standard substances, and parameters of annealing temperature and magnesium ion concentration in a PCR process of the STR gene loci are optimized, wherein the typing standard substances correspond to the allelic genes of the gene loci one by one, so that the microsatellite typing can be performed accurately. The kit of the invention can perform the paternity test and genetic monitoring accurately on species groups of captive giant pandas, complete genealogy, reorganize blood relationship and provide very useful molecular markers for the study in the aspects such as the molecular evolution of the giant pandas, the population migration and the behavioral ecology of wild giant pandas and the like in future, and has very significant theoretical and practical significance on the formulation and implementation of the conservation and breeding plans of rare species.

A mutant type Pfu DNA polymerase and a preparing method and application thereof are disclosed. The amino acid sequence of the mutant type Pfu DNA polymerase is shown as SEQ ID NO:1. The mutant type Pfu DNA polymerase has greatly improved DNA polymerase amplification speed, extending capability and fidelity, and is suitable for various types of conventional PCR, high-fidelity PCR, long-fragment PCRand PCR with a high-GC template. In addition, the mutant type Pfu DNA polymerase has high tolerance to PCR inhibitors in blood, and a blood sample can be directly utilized for PCR detection without the need of DNA purification in advance, thus saving time, and avoiding cross contamination during nucleic acid extraction. The mutant type Pfu DNA polymerase is suitable for direct DNA amplification in anticoagulated blood or blood collected by conventional filter paper, and is suitable for genetic disease diagnosis and paternity tests.

The invention discloses an oval pompanos trevally family paternity test method. For an embodiment for preparing mixed families through multiple parents, 11 pairs of micro-satellite primers which are high in genetic diversity and stable in amplification efficiency are screened out. The purpose of detecting the individual parent-child relationship of an oval pompanos trevally family is achieved in combination with capillary electrophoresis sequencing and software analysis. By means of the method, the oval pompanos trevally paternity test accuracy rate can reach over 90%.

The invention discloses a paternity test method of common suckers. The method comprises the following steps of: (1) extracting DNA from common sucker individuals; (2) screening microsatellite markers of the common suckers; (3) carrying out synthesizing and PCR (polymerase chain reaction) amplification on the microsatellite fluorescence markers of the common suckers; and (4) creating paternity test technology of the common suckers. A paternity test kit of the common suckers comprises 10mM / L of 10*PCR Buffer, 25mmol / L of MgCl2, 10mM / L of dNTPs, 10mM / L of 17 microsatellite markers (F.R), 5U / microliter of rTaq Enzyme and ddH2O. The paternity test method and the kit of common suckers disclosed by the invention can be used for realizing artificial reproduction of common sucker farms, providing reference for creating a common sucker family tree, guiding the artificial fecundation and release of the common suckers and improving the reproduction survival rate of the common suckers.

The invention provides a multiplex amplification kit containing 33 loca of a human genome. The multiplex amplification kit contains 18 A-STR loca which are recommended by a DNA database of the Ministry of Public Security to be used and also contains 14 Y-STR loca which are low in mutation rate and high in polymorphism and the Amelogenin locus, simultaneous amplification and detection on the 33 loca through a single tube is achieved, and synchronous amplification and detection on autosomes and Y chromosomes are achieved in a single experiment. The kit can directly amplify blood stains and saliva stains which take filter paper and an FTA filter as carriers without needing the template extraction and purification process and also can be suitable for DNA samples extracted through different extraction methods. The kit can be used for rapid investigation of a case, can improve the detection efficiency and increase the case investigation speed and especially has the significant effect on male sample trace detection in a raping case and father-child paternity test detection. The kit is wide in application range, good in compatibility and completely compatible with an existing legal medical expert DNA detection system.

The invention discloses a fluorescent detecting method for X chromosome STR gene site typing, which comprises the following steps: allele typing standard substance preparation: carrying out RCP amplification by respectively taking plasmids in different gene types, which comprise an X chromosome STR gene site DXS7132, an X chromosome STR gene site DXS6799 and an X chromosome STR gene site DXS6804 as a template and taking a primer pair of which the 5' ends are marked with fluorescent molecules as primers, and mixing fragments of all alleles in the equal ratio according to molecular weight so as to obtain an allele typing standard substance; carrying out RCP amplification on three X chromosome STR alleles contained in an X chromosome to be detected in the same amplification system; and carrying out capillary electrophoresis separation on the allele typing standard substance with a fluorescent molecule mark obtained by the RCP amplification and the STR allele fragments of the X chromosome to be detected so as to perform standard typing in fluorescent detection typing. The invention is applied to paternity test, individual recognition, sex identification, X linkage inheritance virulent gene positioning and susceptibility gene detection.

The invention discloses specific primers of an acanthopagrus latus microsatellite marker. The specific primers include 8 pairs of primers: AL15, AL20, AL51, AL01, AL37, AL18, AL14 and AL49; the primers have high polymorphism, and the PCR product is stable and reliable. The invention also discloses a microsatellite paternity testing method of acanthopagrus latus, wherein a paternity testing molecular method of acanthopagrus latus is established for the first time, and the method has the advantages of accurate test and low price; the invention further discloses application of the specific primers of the acanthopagrus latus microsatellite marker in the paternity test of acanthopagrus latus.

The invention provides a fluorescently-labeled X-InDel locus composite amplification system. By means of the system, eighteen insertion-deletion genetic polymorphism loci on an X chromosome can be compositely amplified, wherein the eighteen loci are respectively labeled with three fluoresceins FAM, HEX and TAMRA. The composite amplification system is high in sensitivity and individual identification rate, is high in polymorphism, is good in stability and repeatability, is accurate in typing results and can satisfy a practical requirement. A kit can be manufactured on the basis of the composite amplification system, can be used for paternity test, dyad paternity test, grandparent and grandchild test, sibling test and individual identification. New technologies can be provided for the fields such as anthropology, medical genetics and the like.

The invention discloses a primer system used for a sweet potato paternity test as well as a screening method and a kit of the primer system. Specifically, the screening method comprises the following steps: (1) SSR molecular marker design; (2) DNA extraction; (3) PCR amplification and (4) electrophoretic separation; at last, choosing a primer pair having high polymorphism, good repeated stability and hereditary stability, and a clear amplification strip to make up the primer system for a sweet potato paternity test. The primer system locus screened out by the method has the advantages of high co-dominance and polymorphism, good repeatability, clear strip, high hereditary stability and the like. By taking the advantages of a polymorphic primer with a sweet potato microsatellite molecular marker to conduct the paternity test for sweet potatoes, the method has a wide application prospect in fields such as the judgment and discrimination of genetic relationship of the sweet potatoes, the protection of the genetic relationship, property right dispute and the like.

The invention discloses a composite PCR amplification system and an assay kit for genotyping of an STR mark detection system for individual identification and paternity test of yaks. The primer with afluorescence mark can achieve amplification of 15 STR mark sites of yaks, the mark sites are unlocked and have high heterozygosity and rich content of polymorphism information, and amplified productsare of right size. According to a detection method, genotyping results of 15 STR sites are obtained through amplification of 15 pairs of primers and electrophoresis detection; and individual identification and paternity test of yaks are carried out according to the STR locus genotyping results and gene frequency of each STR of yaks. Since individual identification and paternity test of yaks can be carried out effectively by comprehensively analyzing the STR locus genotyping results, accuracy of genealogy in yak breeding can be improved and the breeding process can be accelerated; accordingly,the composite PCR amplification system and the assay kit have good application prospect and economic value.

The invention discloses a method for screening megalobrama amblycephala group combinations with hybrid vigor. The method comprises the following steps of: a, collecting wild parents of the megalobrama amblycephala in an original production area, and matching and breeding; b, culturing filial generations in the same pond in a mixed manner, and measuring relevant growth characteristics of the filial generations after 18 months; c, identifying the source of parents of the filial generations based on microsatellite markers; and d, based on a paternity test and growth characteristic data, analyzing the group combinations with the hybrid vigor, and finding that weights, lengths and heights of the filial generations in three combinations are obviously higher than that of other combinations. Different sources of wild parents are subjected to group combination breeding; a parent gene pool is enlarged; more gene types are collected; the hybrid vigor is sufficiently utilized; and the filial generations grow faster, thus, the megalobrama amblycephala selective breeding effect is more obvious.

The invention belongs to the technical field of research on molecular genetics and particularly relates to a method for utilizing a microsatellite marking technology to perform a paternity test on goats and a microsatellite primer and a kit thereof. The invention discloses a paternity test method for the goats, which comprises the following steps of: (1) extracting a blood sample from a goat breed and performing DNA (Deoxyribonucleic Acid) extraction and detection; (2) selecting a microsatellite locus: selecting the microsatellite locus with a stable PCR (Polymerase Chain Reaction) augmenting result and a clear strip as well as a polymorphism but without non-specific augmentation; (3) utilizing each microsatellite locus to perform PCR augmentation on different goat DNA templates, and performing electrophoresis detection on augmenting fragments; and (4) performing genetic polymorphism analysis on the microsatellite locus. The invention also discloses the microsatellite primer and the kit used for the paternity test method for the goats. The paternity test method for the goats, provided by the invention, is simple in operation, high in speed, low in cost, and high in accuracy.

The invention relates to the technical field of biology, and relates to a composite amplification system of 28 short tandem repeats, a kit and an application thereof. The composite amplification system comprises 28 pairs of primers, and can be used for amplifying 28 sites at the same time; and the invention also discloses a primer sequence aiming at the 28 sites. By means of a research of geneticdiversity of a STR locus, 28 sites which contain 22 new CODIS sites, 4 sites with high discrimination power (D4S2366, D6S1043, D11S2368, D14S608) and one gender recognition site Amel, and one Y chromosome insertion-deletion site Y-indel are selected. The sites have higher discrimination power, excluding probability of paternity and polymorphism information content, and other characteristics, so that paternity test and discrimination power system effectiveness can reach a higher level.

The invention relates generally to polymorphisms or mutations of the PHOX2B gene. More particularly, the invention relates to polymorphisms or mutations of the PHOX2B gene that are responsible for the disease Hirschsprung's disease (HSCR), which is a neural crest-associated developmental disorder. Specifically, the invention relates to the detection of a single base-pair polymorphism in the PHOX2B gene that is associated with HSCR. The invention also relates to methods and kits for screening for carriers of mutations of the PHOX2B gene and the diagnosis of increased risk of HSCR. The invention further relates to diagnosing predisposition or susceptibility to increased risk of developing HSCR by screening for the presence of a polymorphism associated with HSCR. The invention also relates to compositions for screening for the polymorphism and treatment choices for patients having the polymorphism of the present invention. The invention further relates to providing polymorphisms in the PHOX2B gene for forensic use and in paternity test. The invention also relates to screening assays and therapeutic and prophylactic methods.

The invention discloses a multiplex system, a method for detecting unbalanced mixed samples through the multiplex system and application of the method. The multiplex system comprises primers for amplifying 18 SNP-STR (single nucleotide polymorphism-short tandem repeat) sites, each site comprises a common reverse primer and two specific forward primers for increasing mutation, and the specific sequences of the primers are as shown in the SEQ ID NO.1-54 in the specification. The SNP-STR (single nucleotide polymorphism-short tandem repeat) multiplex amplification system has the advantages that the operation process is simplified, the system can be widely used on the basis of existing forensic evidence conventional inspection equipment, mixed samples of two persons at a forensic scene are detected, the same STR (short tandem repeat) typing samples are distinguished, non-invasive prenatal diagnosis and non-invasive prenatal paternity test are realized, a new technical means is provided, andclues and evidence are provided for solving a case.

Disclosed are an SSR fluorescent labeled primer for paternity test of pangolin and application thereof. 15 pairs of fluorescent labeled satellite primers are successfully screened and synthesized, theprimers can be used in pangolin paternity test, be used for making pangolin paternity testing kit, and be used for pangolin germplasm test and pedigree management, which can guide pangolin breeding and provide a powerful tool for protecting pangolin genetic diversity and population genetic institutions. The invention also provides a method for paternity testing of pangolin. In the method, an M13linker is added, and only one M13 fluorescent label primer is synthesized to carry out PCR expansion, thereby saving the cost and time of synthesizing a large number of fluorescent primers.

The invention discloses a procypris rabaudi Tchang test kit and a microsatellite PCR identification method thereof. The method comprises the steps of extracting genome DNA of a procypris rabaudi Tchang individual sample; performing PCR amplification; marking the 5'end of a forward primer of each pair of 20 microsatellite primers in a procypris rabaudi Tchang paternity test primer group with a fluorescent substance, performing gradient PCR amplification on the obtained DNA genome by using each marked fluorescent primer in the primer group, performing capillary electrophoresis on an amplification product on a sequencer, reading the allele size of each individual, and obtaining genotyping data; and performing paternity test analysis of the procypris rabaudi Tchang family: performing parent and filial generation data analysis based on the genotype data, and determining the paternity relationship between the filial generation and the parent according to the correlation between the filial generation genotype and the parent genotype. The method is simple, quick, efficient and economical, and can provide a technical approach for family management, population genetic variation research andproliferation and release effect evaluation of procypris rabaudi Tchang breeding populations.