35 results about "Rare species" patented technology

The invention relates to a method for constructing a deep pool and a shoal in a straight river channel. In the method, a spur dike is arranged on the left bank side of the straight river channel, a bank slope on the opposite bank of the spur dike is protected by riprap, a diving guide wall is arranged on the left and right banks of the downstream side of the spur dike respectively, the discharge cross section of the river channel is shortened by the spur dike to increase the flow rate of water flow, the bottom of a river bed of a river reach between the diving guide walls on the downstream side of the spur dike is continuously scoured to naturally form the deep pool, and a dead water area or slow flow water area formed between the diving guide wall and the river bank becomes the shoal through long-term deposition. The method has the advantages that: the deep pool is naturally formed and becomes a rest place for aquatic organisms such as fishes and the like, and can become a shelter for the aquatic organisms such as the fishes and the like in a flood period; plants can be planted on the shoal, and the shoal can become an organism habitat different from the deep pool; and the complexity of a river channel structure and water conservation condition diversity can be increased or repaired, and river biodiversity, population composition and rare species conservation are facilitated.

The invention relates to a circular agriculture system and an implementation method thereof. According to a characteristic that different biological wastes can be highly efficiently cycled by various insects and small animals, an insect breeding factory is constructed; and insect and small animal proteasome and insect feces residue organic fertilizer are respectively utilized as an animal factory protein feed and a plant factory organic fertilizer. Therefore, carious animal and plant products with high added values can be produced with high yields. With modern advanced agricultural technologies and equipment, stereo cultivation is usually carried out with rare species, such that a unit yield of the types of factories reaches more than a hundred times that of conventional agriculture. With the products from the three types of factories, a wide variety of series downstream industry chain group can be developed, such that best economic benefit can be ensured. The three types of factories, downstream industry chain spaces and farmer residential spaces can be arranged in a rise building. The buildings can form a highly clustered production, management, residence, agriculture, industry and business integral community. With farmland novel industry and landscape designs outside the buildings, a novel intensive town is formed.

The invention discloses a kit used for a paternity test of giant pandas. 68 allelic genes of 11 STR gene loci, obtained by microsatellite study, of the giant pandas are taken as typing standard substances, and parameters of annealing temperature and magnesium ion concentration in a PCR process of the STR gene loci are optimized, wherein the typing standard substances correspond to the allelic genes of the gene loci one by one, so that the microsatellite typing can be performed accurately. The kit of the invention can perform the paternity test and genetic monitoring accurately on species groups of captive giant pandas, complete genealogy, reorganize blood relationship and provide very useful molecular markers for the study in the aspects such as the molecular evolution of the giant pandas, the population migration and the behavioral ecology of wild giant pandas and the like in future, and has very significant theoretical and practical significance on the formulation and implementation of the conservation and breeding plans of rare species.

The invention relates to a propagation method of fruit trees, and discloses a propagation method of wufu persimmon seedlings. The method comprises the steps of cleaning and disinfecting wufu persimmonpulp and then preparing the wufu persimmon pulp into fruit leaven; putting wufu persimmon seeds into the fruit leaven to be soaked, and promoting seed germination and rooting; after transplanting, proportioning quantitative clean water for the fruit leaven, watering bud seedlings periodically, using the fruit leaven to provide original nutrients for seedlings, and finally enabling the seedlings to grow into normal and robust plants. In this way, the endangered rare species is rescued. By means of the propagation method of the wufu persimmon seedlings, the survival rate of the wufu persimmon seed-raising seedlings exceeds 80%, the limit of non-propagation of the wufu persimmon seedlings is broken through, and the propagation method is significant in protecting, studying, protecting, applying and popularizing the species.

This invention provides a social network for wildlife species observers, e.g. birders, to verify accuracy of observations made of wildlife in the field, and includes issuance of automatic rare species alerts when a sighting is determined to be rare, all via a web site.

The invention relates to a method for field culture of cordyceps sinensis and vegetation recovery in alpine-cold cordyceps growing areas. Cordyceps sinensis is an insect herb which is formed by hepialus larvae (host) after being infected by fungus cordyceps sinensis and is a special product of a biological chain of fungus, insects and plant in a special habitat. The invention belongs to Field Culture of Wild Medicine biological engineering and has the advantages of proliferating fungus and the hosts in alpine-cold road and field growing areas, increasing and protecting alpine meadow and alpineshrub and meadow vegetation, compensating the activity of the biological chain, developing geographical (biological) resources of cordyceps sinensis habitat which is difficult to simulate by human, decreasing natural enemy hazard to protect the rare species of cordyceps sinensis with stable production and good quality, and protecting and improving the fragile alpine meadow vegetation. The methodadopts different species of hosts for egg-increasing assistance by levels and regions to increase the hatching rate and the larva density; the fungus is separated and purified, and the fungus source is provided through ferment, so that the infection rate of insects by fungus is improved; meanwhile, the favorite plant of host larva is compensated, and growth regulator and special fertilizer are applied to protect the alpine meadow vegetation.

The invention discloses a planting method used for gastrodia elata blume in the technical field of traditional Chinese medicinal material planting. The planting method comprises the following steps that firstly, seed dressing is conducted, wherein gastrodia elata blume seeds are mixed with germination bacteria; secondly, a tree species is treated, wherein a through hole is formed in the circle center of a tree trunk, and a plurality of bacteria containing holes are formed in the periphery of the tree trunk; thirdly, nutrient materials are prepared, wherein branches of Chinese chestnut trees, sucrose, corn cobs and radix notoginseng are selected and mixed to obtain fine mixture powder, and the fine mixture powder is heated and decocted into paste; fourthly, inoculation is conducted, whereinthe paste obtained in the third step is injected into the through hole in the tree trunk in the second step, and meanwhile, the mixed seeds in the first step are inoculated into the bacteria containing holes to obtain culture wood; fifthly, planting is conducted, wherein a planting pit is dug in planting land, the culture wood in the fourth step is placed in the pit, then cordate houttuynia is placed, and the pit is covered with sand and then covered with a layer of leaves; sixthly, daily management is conducted, wherein watering is conducted in time; seventhly, harvesting is conducted, wherein after 240-300 days, mature gastrodia elata blume is harvested. The planting method is beneficial to the growth of the gastrodia elata blume.

The invention discloses a transplantation method for Michelia guangdongensis. The transplantation method comprises the following steps of (1) digging tree caves, (2) trimming the Michelia guangdongensis, (3) digging seedlings, (4) wrapping the seedlings, (5) transporting the seedlings, (6) fixed-planting the seedlings and (7) performing management after plantation. Growth features of the Michelia guangdongensis are combined with transplantation technology and culturing technology, and the Michelia guangdongensis can be quickly suitable for soil and environment of the transplantation land and quickly root, emergence leaves, restore tree vigor and achieve greening effect; more than 90% of survival rate can be achieved; the Michelia guangdongensis, rare species can be effectively protected; and the transplantation method is advantaged by simple operation and easy promotion and application.

The invention relates to a Sinocyclocheilus anshuiensis spleen cell line construction method. The construction method comprises the following steps: (1) obtaining a Sinocyclocheilus anshuiensis spleen: directly disinfecting a fish body by adopting ethyl alcohol; (2) performing primary culture: culturing by adopting B-15 culture solution containing glutamine, fetal calf serum, penicillin, streptomycin, gentamicin and amphotericin B; (3) subculturing: when culturing to the eighth generation, changing the cell culture solution into basic culture solution, thus successfully constructing a Sinocyclocheilus anshuiensis spleen cell line. The Sinocyclocheilus anshuiensis spleen cell line obtained by the construction method can realize continuous passage, is directly applied to biological characteristic research, and can meet the requirements of germplasm resource conservation and theoretical research on Sinocyclocheilus anshuiensis as a rare species; more importantly, the cell line is the first cell line of cave fish in China, and lays the foundation for the cellular level cave adaptability research.

The invention relates to a library construction method applicable to a cell line and animal tissue ATAC-seq sequencing technology. The method comprises steps as follows: 1) cell or animal tissue is collected, and a cell suspension is prepared; 2) a lysate containing NP40 is added to split cell membranes, and cell nucleuses are obtained and purified; 3) the cell nucleuses are collected for quantitative and integrity detection; 4) Tn5 transposase is added to complete cell nucleuses for a transposition reaction and purification; 5) PCR amplification is performed, and a second library is constructed and purified; 6) high-throughput sequencing is performed, and gene data are obtained. According to the method, quantitative operation is performed directly through the DNA content on the basis of the characteristic that transposase is sensitive to DNA content, and the method is wide in applicability and particularly applicable to library construction of rare species or tissue and tissue in poorpreservation state. Meanwhile, the recycled cell nucleuses can be used for Tn5 transposase developed by domestic vazyme company through early optimization, good experiment results are obtained, and ATAC-seq is not limited by the variety of transposase any more.

The invention relates to a rare species DNA information acquiring method, and belongs to the technical field of measuring or detecting methods containing enzyme or microorganisms. The method comprisesthe following steps: after acquiring a tissue of a south China tiger, purifying DNA, and enabling the DNA to generate random fragmentation, separating the DNA, and recovering, performing a A-adding catalytic reaction on the recovered DNA, and performing a coupled reaction on a T-carrier, performing conversion on a coupled product, screening a positive bacterial colony, culturing overnight, performing plasmid recovery, sequencing, and performing a specific primer design, synthetizing, and implementing PCR again and sequencing a PCR product, performing comparison with a sequence used in the primer design, if a result is consistent, it is demonstrated that the DNA fragment is the DNA of the target species surely. The method is used for acquiring DNA information of the rare species, and has the advantages of simple and convenient operation, high acquiring rate, low cost and the like.

The invention provides a new strain of Agaricus bernadii Mengbaiyin No.2. The new strain of Agaricus bernadii Mengbaiyin No.2 is cultivated from wild Agaricus bernadii which is commonly known as Agaricus arvensis, and has a preservation number of CGMCC No.15086. The invention also provides a method for preparing and cultivating a strain of the wild Agaricus arvensis. The method comprises preparation of the strain, propagation, preparation of original seeds and preparation and cultivation of cultivated seeds. Not only can the endangered rare species of Agaricus arvensis be protected so as to develop a new economic growth point besides grass and livestock for an pastoral area, but also manure of cattle and sheep and grass in the pastoral area can be turned into treasure; in summer, local herders can use idle cattle and sheep pens to plant the Mengbaiyin No.2 to increase income, so that a new way of an ecological economy in the pastoral area is developed.

The invention provides a kit and an identification method for molecules of five Sinipercinae fishes. The kit comprises three pairs of SSR primers and standard maps. The identification method for molecules of five Sinipercinae fishes by using the kit comprises the following steps: 1) DNA extraction: a step of acquiring the fin ray or muscle tissue of a to-be-tested Siniperca chuatsi sample, extracting genome DNA and measuring the purity and concentration of the genome DNA; 2) DNA fragment amplification: a step of amplifying the genome DNA of the to-be-tested sample by using a polymerase chain reaction; 3) electrophoresis and silver staining identification of PCR products: a step of carrying out electrophoretic separation on the PCR products by using nondenaturing polyacrylamide gel, then carrying out staining by using a conventional silver staining method and taking photos to record electrophoresis results; and 4) comparison with the standard maps. The kit and the identification method provided by the invention can substantially improve efficiency and accuracy of identification of important economic and rare varieties of Sinipercinae and are beneficial for protection of genetic resources of Sinipercinae fishes and sustained sound development of the breeding industry of Siniperca chuatsi.

The invention discloses efficient taxol-producing endophytic fungi aspergillus aculeatus Tax-6 and application thereof. The efficient taxol-producing endophytic fungi has the classification and name of Aspergillus aculeatus Tax-6 and is preserved in China Center for Type Culture Collection with the preservation number of CCTCC M 2016614, the preservation date of November 3rd, 2016 and the preservation address of Wuhan University in Wuhan, China. According to the invention, Tax-6 is cultivated by virtue of a liquid fermentation technology, then taxol is extracted from fermentation broth, the highest content reaches 334.92mu g/L, and cultivation time is only 7 days. The endophytic fungi on the bark of taxus chinensis are utilized for carrying out fermentation and producing taxol, an efficient anti-cancer drug taxol is obtained with low cost, and the rare species taxus chinensis is protected.

The invention relates to a method for constructing a sinocyclocheilus rhinocerous heart cell line. The method comprises the following steps: 1, obtaining of sinocyclocheilus rhinocerous heart tissues, wherein sinocyclocheilus rhinocerous is sterilized through combination of penicillin and ethyl alcohol; 2, primary culture, wherein a culture solution M199 which contains glutamine, penicillin, streptomycin, streptomycin sulphate and amphotericin is adopted for culture, and the osmotic pressure of the culture solution is 280-330 mOsm/l; 3, secondary culture, wherein when the tenth generation is reached, cell culture fluid is replaced with base culture fluid, and the sinocyclocheilus rhinocerous heart cell line is constructed successfully. According to the method for constructing the sinocyclocheilus rhinocerous heart cell line, the obtained sinocyclocheilus rhinocerous heart cell line is in the fibrous form and can conduct continuous passage and be directly applied to research of biological characteristics, requirements of genetic resource preservation and theoretical research conducted on the rare species, namely the sinocyclocheilus rhinocerous, can be satisfied, meanwhile, the heart cell line is the first heart cell line of cave fishes in China, and the foundation is laid for cave adaptability research at the cellular level.