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Efficient taxol-producing endophytic fungi aspergillus aculeatus Tax-6 and application thereof

A technology of Aspergillus aculeatus and paclitaxel, which is applied in the directions of fungi, microorganisms, microorganisms, etc., can solve the problems of low yield of endophytic fungi and can not achieve industrialized production, and achieves short fermentation culture period, good practicability, and good industrialization prospects. Effect

Active Publication Date: 2017-08-18
NANJING FORESTRY UNIV
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Problems solved by technology

However, the current bottleneck of this technology lies in the low yield of the screened endophytic fungi, which cannot reach the level of industrial production

Method used

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  • Efficient taxol-producing endophytic fungi aspergillus aculeatus Tax-6 and application thereof
  • Efficient taxol-producing endophytic fungi aspergillus aculeatus Tax-6 and application thereof
  • Efficient taxol-producing endophytic fungi aspergillus aculeatus Tax-6 and application thereof

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Embodiment 1

[0025] Liquid fermentation culture: take out from the refrigerator at 4°C and store Aspergillus aculeatinus The PDA plate of Tax-6 was placed in a biochemical incubator at 28°C, and the strains were activated for 2-3 days. On the sterilizer, add about 20 mL of sterile water to the activated plate, gently scrape the spores on the mycelium into the sterile water with an inoculation needle, mix well, and filter aseptically to make a spore suspension. liquid (about 1 x 10 7 spores / mL). Draw 2 mL of spore suspension with a sterile pipette tip and inoculate it into a 250 mL Erlenmeyer flask containing 100 mL of PDA solid medium, and place the inoculated Erlenmeyer flask on a shaker at 120 rpm at 30°C for cultivation.

[0026] Extraction of paclitaxel: filter the fermentation broth with four layers of gauze to take the liquid part, add 10 mL of n-hexane to extract for 20 min to remove impurities such as fatty acids, and keep the water phase. Paclitaxel in the fermentation broth ...

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Abstract

The invention discloses efficient taxol-producing endophytic fungi aspergillus aculeatus Tax-6 and application thereof. The efficient taxol-producing endophytic fungi has the classification and name of Aspergillus aculeatus Tax-6 and is preserved in China Center for Type Culture Collection with the preservation number of CCTCC M 2016614, the preservation date of November 3rd, 2016 and the preservation address of Wuhan University in Wuhan, China. According to the invention, Tax-6 is cultivated by virtue of a liquid fermentation technology, then taxol is extracted from fermentation broth, the highest content reaches 334.92mu g / L, and cultivation time is only 7 days. The endophytic fungi on the bark of taxus chinensis are utilized for carrying out fermentation and producing taxol, an efficient anti-cancer drug taxol is obtained with low cost, and the rare species taxus chinensis is protected.

Description

technical field [0001] The invention relates to the technical field of drug production by plant endophytic fungi, in particular to a high-efficiency paclitaxel-producing fungus Aspergillus aculeatus Tax-6 and its application. Background technique [0002] Cancer is one of the three major killers threatening human health in the 21st century. According to the survey by the National Cancer Registry Center, since 2013, there have been more than 3 million new cancer cases in my country every year. Therefore, the task of finding highly effective anticancer drugs is also imminent. Paclitaxel ( Taxol ) originally from the genus Taxus ( Taxus spp. ) is a diterpenoid isolated from plants with a unique anticancer effect. It acts on cell microtubules and induces and stabilizes microtubules by binding to amino acids 31 and 217-231 at the N-terminal of tubulin. Tubulin polymerization, inhibit its depolymerization, increase the polymerization speed, so that the vascular bundle can not b...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/02C12R1/66
CPCC12P17/02C12N1/145C12R2001/66
Inventor 乔维川凌飞谢震宇夏昊葛秀秀柏涵刘海王婷
Owner NANJING FORESTRY UNIV
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