79 results about "Aspergillus aculeatus" patented technology

The invention relates to a compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is characterized by being prepared from the following materials in percentage by weight: 40.0-50.0 % of rape seed cakes, 10.0-20.0% of crops straws, 1.5-3.0% of mycothallus, 0.5-1.0% of bacterium thallus and 35.0-40.0% of water. By using the solid fermentation method twice, the invention organically combines the fungi (Trichoderma harzianum and Aspergillus aculeatus for biological control effect) and the bacteria (Bacillus subtillis and Bacillus megatherium for growth-promoting effect), and realizes a simple preparation method. The compound microbial fertilizer has the advantages of environmental condition resistance, high count of active viable bacteria, less influence by the environment after application, slow release of thallus and long-term effective colonization at rhizosphere of plants, promotes plant growth and simultaneously has a certain biological control effect on soil-borne diseases.

The invention relates to a composite microbe fertilizer. The composite microbe fertilizer is prepared by 25-35 wt% of vegetable garden soil, 5-10 wt% of active functional bacteria, 5-15 wt% of mineral powder, 25-30 wt% of an organic fertilizer, 15-20 wt% of an inorganic composite fertilizer, and water; wherein the sum of the components is 100%. The active functional bacteria comprise lactic acid bacteria, bacillus subtilis, Bacillus megaterium, trichoderma, aspergillus niger, monascus, Trichoderma harzianum, aspergillus aculeatus and yeast, the mineral powder is a mixture of one or more of peat, zeolite, vermiculite, montmorillonite, calcium carbonate and expanded perlite, fineness of the mineral powder is less than or equal to 200 meshes, the organic fertilizer contains decomposed manure, a rapeseed cake fertilizer, and straw, and the weight ratio of the manure to the rapeseed cake fertilizer to straw is 5.5-7.5:2.5-3.5:0.5-1.5.

The invention discloses a method for utilizing cadmium-resisting fungus aspergillus aculeatus for promoting festuca arundinacea to remedy cadmium contaminated soil, and relates to the technical field of plant-microorganism combined ecological remediation. The method mainly comprises the steps that firstly, cadmium-resisting fungus aspergillus aculeatus separated, purified and identified out of rhizosphere soil of dominant species in heavy metal contaminated soil is bred and inoculated into crushed crop straw to be prepared into a microbial remediation matrix, and the microbial remediation matrix is applied into cadmium contaminated soil; secondly, a festuca arundinacea lawn is planted in a sowing manner; thirdly, watering of a citric acid aqueous solution of a proper concentration and a proper quantity is carried out in batches; and fourthly, the rhizosphere microenvironment of festuca arundinacea is adjusted and controlled through citric acid to promote the interaction of cadmium-resisting fungus aspergillus aculeatus and a root system of festuca arundinacea so as to improve cadmium remediation efficiency. The problem of adaptability of screened-out microorganisms to the soil environment is solved; transferring of cadmium towards ground parts of plants is promoted, and the cadmium remediation efficiency is improved; and good environmental benefits, economic benefits and social benefits are achieved.

The invention discloses a method for remedying cadmium-contaminated soil through turfgrass and microorganism combination, and relates to a heavy metal-contaminated soil treatment technology. The method comprises the following steps: (1) screening cadmium-resistant bermuda grasses so as to obtain the bermuda grasses with strong cadmium resistance; (2) collecting rhizosphere soil of the bermuda grasses in a contaminated area; (3) screening cadmium-resistant microorganisms so as to obtain the microorganisms with the strongest cadmium resistance, which are fungi-aspergillus aculeatus; (4) preparing microorganism remedying matrixes; and (5) carrying out turfgrass and microorganism combined remediation. The method has the advantages that the dominant plants, namely the bermuda grasses in the cadmium-contaminated area are used as cadmium-resistant plant screening sources and have strong adaptability to the soil environments in the contaminated area; the cadmium-resistant fungi-aspergillus aculeatus is derived from the rhizosphere soil of the bermuda grasses in the cadmium-contaminated area, is well compatible with the cadmium-resistant bermuda grasses and has strong adaptability to the soil environments in the contaminated area; the microorganism remedying matrixes can be used for ensuring the nutrient supply of microorganism growth, enhancing the operability of applying the microorganisms in soil and improving soil environments, and the waste is effectively utilized; the method is low in cost and simple and convenient to operate.

A saline soil improver is characterized by comprising 25%-35wt% of vegetable soil, 5%-10wt% of active functional bacteria, 5%-15wt% of mineral powder, 25%-30wt% of an organic fertilizer, 15%-20wt% of an inorganic compound fertilizer and balance of water, the active functional bacteria includes lactic acid bacteria, bacillus subtilis, bacillus megatherium, trichoderma, aspergillus niger, Monascus, Trichoderma harzianum, Aspergillus aculeatus and yeast, the mineral powder is one of or a mixture of more than one of peat, zeolite, vermiculite, montmorillonite, calcium carbonate and expansion perlite, the mineral powder fineness is less than or equal to 200 meshes. The organic fertilizer contains composted manure, a rapeseed cake fertilizer and straws, and the weight ratio of composted manure to rapeseed cake fertilizer to straws is 5.5-7.5: 2.5-3.5: 0.5-1.5.

The invention relates to an Aspergillus aculeatus ZJ bacterial strain and an application thereof. The collection number of the Aspergillus aculeatus ZJ is CCTCC NO.M2011264. The bacterial strain is mainly used for converting effective components of the pod of Chinese scholartree to produce 5,7,8,4'-tetrahydroxyisoflavone. The specific method provided by the invention comprises the following steps of: acting the Aspergillus aculeatus ZJ with strong specificity or the produced enzyme on the pod of Chinese scholartree or extracting solution thereof; converting glucosides compounds including sophoricoside and the like, genistein and the like to produce the 5,7,8,4'-tetrahydroxyisoflavone. The invention provides the method for producing the 5,7,8,4'-tetrahydroxyisoflavone through utilizing the Aspergillus aculeatus with high specificity and high conversion efficiency to convert the sophoricoside; and the method provided by the invention has moderate conditions, green process and simple requirements on the process and the device, and has a very good application value.

The invention relates to an aspergillus aculeatus strain and application thereof, and belongs to the technical field of fermentation engineering and biology. The preservation name of the aspergillus aculeatus strain is (Aspergillus sp.) BG30, the preservation department is China microorganism culture preservation management committee common microorganism center, the preservation data is June 28th, 2013, and the preservation number is CGMCC NO.7799. During fermentation of the aspergillus aculeatus strain, the enzymatic activity of ectoenzyme beta-glucosidase is high, by-products are few, the beta-glucosidase is prone to separation and purification, the separation and purification cost is low, and therefore the fact that the aspergillus aculeatus strain serves as original strain to ferment and produce beta-glucosidase facilitates industrial production.

The invention belongs to the technical field of plant protection, in particular to an Aspergillus echinosporus strain and application thereof, and relates to biological control of soil-borne diseasesof vegetables. Aspergillus aculeatus (Aspergillus aculeatus) Aa19 is isolated from rhizosphere soil collected from vegetable plots where soil-borne diseases are serious. The strain is deposited in China Center for Type Culture Collection under the accession number CCTCC NO: M 2018648. Aspergillus aculeatus (Aspergillus aculeatus) Aa19 is isolated from the rhizosphere soil of vegetable plots wheresoil-borne diseases occurred. The stability of the strain of Aspergillus aculeatus, the activity of the spore suspension and the activity of the fermentation filtrate were tested by the reference number CWSC NO:M 2014314 for the Fusarium oxysporum JWS-1. The biological function verification proves that the isolate of the invention has good control effect on soil-borne diseases of vegetables. Aspergillus acanthosporus Aa 19 is safe to vegetables, has good stability and high bacteriostatic activity, and can be used for biological control of soil-borne diseases-cowpea wilt.

The invention discloses a joint relieving method for the stress damage of nanometer zinc oxide to plants by utilizing aspergillus aculeatus fungus and salicylic acid. The method comprises the steps that plant seedlings growing robustly are selected and transplanted into soil containing nanometer zinc oxide, aspergillus aculeatus fungus liquid and a salicylic acid solution are added to the rhizosphere of plants, and growth is achieved through culture. According to the method, the raw materials are easy to obtain, and the method is simple, easy to implement and low in cost and has a good application prospect. It is proved through an experiment that the aspergillus aculeatus fungus and the salicylic acid are utilized for jointly relieving the stress damage of the nanometer zinc oxide to the plants.

The invention discloses aspergillus aculeatus P93, a biofertilizer preparation prepared from same and application thereof. The aspergillus aculeatus P93 has the preservation number of CGMCC NO.14150.Microbial inoculum prepared from the aspergillus aculeatus P93 has obvious effects of promotion of crop growth, yield increasing and activation of soil phosphorus. and the microbial inoculum P93 can dissolve multiple insoluble phosphorus, ground phosphate rock, iron phosphate, zinc phosphate and tricalcium phosphate are efficiently dissolved in a liquid culture medium, and dissolution rate reaches12.1-94.2%; under soil conditions, five insoluble phosphorus sources are used, then the microbial inoculum P93 is inoculated, and soil available phosphorus is improved by 257.8-1004.6%; the microbialinoculum P93 is inoculated for promoting phosphorus absorption of corns, and total phosphorus of a corn plant is increased by 3.4-136.4%; and phosphorus dissolving capability is higher than that of phosphorus dissolving penicillium bilaiae (ATCC 20851) which is reported at present and internationally recognized. The microbial inoculum disclosed by the invention has significance on conservation ofphosphorus resources in agricultural production in China, the sustainable yield increase of cereals and protection of farmland ecological environment.

The invention discloses a preparation method of a phosphate-solubilizing microbial agent and application thereof and belongs to the field of microbial technology. The preparation method of the phosphate-solubilizing microbial agent comprises the following steps: (1) preparation of a liquid state phosphate-solubilizing microbial solution; and (2) preparation of a solid state phosphate-solubilizingmicrobial agent. The application of the solid state phosphate-solubilizing microbial agent comprises the following steps: (1) during building and planting, the solid state phosphate-solubilizing microbial agent is applied by a broadcasting mode, and the application amount is 10-20 kg/Mu; and (2) during sowing, the solid state phosphate-solubilizing microbial agent and seeds undergo seed dressing (1 kg of ryegrass seeds are stirred into 5-8 kg of the solid state phosphate-solubilizing microbial agent), and sowing is conducted. The phosphate-solubilizing microorganism, namely Aspergillus aculeatus has endophilicity with ryegrass, has capabilities of secreting IAA, siderophore, ACC deaminase and organic acid, can effectively promote plant growth while solubilizing phosphate, can improve soilfertility, and can greatly increase content of rapidly available phosphorus in soil. The preparation is convenient, application method is simple, and a broadcasting or seed dressing method, etc. can be adopted for inoculation; and cost is low, and operation is simple.

The invention relates to a sustained-release fertilizer for preventing soil salinization. The sustained-release fertilizer is characterized by being prepared from 25wt% to 35wt% of vegetable garden soil, 5wt% to 10wt% of active functional bacteria, 5wt% to 15wt% of mineral powder material, 25wt% to 30wt% of organic fertilizer, 15wt% to 20wt% of inorganic compounded fertilizer and water, wherein the sum of weight percent of all the ingredients is 100%, the active functional bacteria comprise lactic acid bacteria, bacillus subtilis, bacillus megaterium, trichoderma, aspergillus niger, monascus, trichoderma harzianum, aspergillus aculeatus and yeasts, the mineral powder material is one or a mixture of more of peat, zeolite, vermiculite, montmorillonite, calcium carbonate and expanded perlite, the fineness of the mineral powder material is smaller than or equal to 200 meshes, the organic fertilizer contains decomposed manure, a rapeseed cake fertilizer and straws, and the weight ratio of the manure to the rapeseed cake fertilizer to the straws is (5.5 to 7.5): (2.5 to 3.5): (0.5 to 1.5).

The invention discloses a bagasse saccharifying method which comprises the following steps: (1) pretreatment of bagasse: sterilizing bagasse with infrared ray, and regulating the moisture content of bagasse to 15%-20% through microwave drying or addition of water; (2) saccharification: adding zymophyte in bagasse, then fermenting at the temperature of 25-30 DEG C, stopping fermentation when ethylalcohol is generated, sterilizing bagasse with infrared ray, then adding citric acid, dimethyl sulfoxide tetrabutylammonium chloride and dimethyl sulfoxide imidazolium salt in the sterilized bagasse,and degrading for 2-6 hours at the room temperature, wherein the zymophyte comprises trichoderma reesei, aspergillus niger, aspergillus aculeatus and kluyveromyce; and (3) enzyme deactivation: puttingthe saccharified bagasse at a high temperature of above 100 DEG C for sterilization for 10-30 min. The bagasse saccharifying method has the advantages that the saccharifying rate is high, and the fermentation end-point is easy to control.

The invention relates to a benzoquinone derivative from aspergillus aculeatus and application of the benzoquinone derivative. The structural formula of the compound is shown in the specification. Experiments prove that the benzoquinone compound has good antitumor activity. The benzoquinone derivative can be used for preparing cell proliferation inhibition drugs or antitumor drugs for antitumor study.

The present invention provides for heterologous expression of beta-glucosidase (BGL) polypeptides encoded by Humicola grisea, Candida wickerhamii, Aspergillus aculeatus, Aspergillus oryzae, Penicillium decumbens, Chaetomium globosum, Neocallimastix frontalis, Debaryomyces hansenii, Kluyveromyces marxianus, or Phytophthora infestans in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed BGL. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

The invention discloses a coding gene of an alpha-L-rhamnosidase mutant. The nucleotide sequence of the alpha-L-rhamnosidase mutant is shown as SEQ ID NO:1. According to the invention, site mutation is carried out on the basis of an aspergillus aculeatus wild type alpha-L-rhamnosidase gene; the similarity between the mutated alpha-L-rhamnosidase mutant gene and the wild type alpha-L-rhamnosidase gene is 99.89%; and compared with the wild type alpha-L-rhamnosidase mutant gene, after being expressed in pichia pastoris, the mutated alpha-L-rhamnosidase mutant gene has the advantages that enzyme activity and heat resistance of alpha-L-rhamnosidase are obviously improved. According to the method, catalytic performance and stability of the alpha-L-rhamnosidase under a high-temperature conditioncan be improved.

The invention relates to a benzoquinone spiro compound derived from aspergillus aculeatus and application of the benzoquinone spiro compound. The structural formula of the compound is shown in the specification. Experiments prove that the benzoquinone spiro compound has good antitumor activity. The benzoquinone spiro compound can be used for preparing cell proliferation inhibition drugs or antitumor drugs for antitumor study.

The invention belongs to the technical field of microbial transformation and particularly relates to an Aspergiulls aculeatus mutant strain for producing ratgalacturonorhamnan hydrolase at high yield.According to the Aspergills aculeatus mutant strain disclosed by the invention, Aspergiulls aculeatus DSM2344 is used as an original strain and an ultraviolet mutagenesis method is adopted to selectfor obtaining a mutant strain Aspergiullus aculeatus T13 with a preservation number of CCTCC NO: M2017784. After a mutant strain solid is subjected to solid shake flask fermentation for 3.5 days, theenzyme activity of the ratgalacturonorhamnan hydrolase reaches 63.7u/g, which is increased by 41.6 percent compared with that of an original strain; unexpected technical effects are achieved. The Aspergiulls aculeatus mutant strain provided by the invention can be widely applied to fermentation production of the ratgalacturonorhamnan hydrolase; the production cost of the enzyme is favorably reduced and promotion and application of the ratgalacturonorhamnan hydrolase in the food industry are promoted.

The invention discloses a saccharomyces cerevisiae industrial strain capable of delta integration and secretion and expression of cellulase and an application thereof. The saccharomyces cerevisiae industrial strain has a preservation number of CGMCC NO. 16825. The industrial strain R delat BEC, with the preservation number of CGMCC NO. 16825 is applied to ethanol production. The strain R delta BEC, with the preservation number CGMCC NO. 16825, secretes and expresses five kinds of cellulase: trichoderma reesei CBH1, CBH2 and EG2; aspergillus aculeatus BGL1 and CBH1. The bacteria can cooperate with prior commercial cellulase to efficiently degrade cellulose and improve the yield of ethanol.

The invention discloses a honey pomelo sac stomach nourishing and fermenting beverage and relates to the technical field of preparation of honey pomelo beverages. A preparation method comprises the following steps of debittering pomelo sac powder: by using aspergillus aculeatus, aspergillus niger, aspergillus terreus and the like as strains and using corn steep liquor as a nitrogen source, adding water, and then fermenting the pomelo sac powder to produce naringinase, wherein the ratio of mass fraction of the honey pomelo sac powder to the corn steep liquor to the water is 100:(10 to 30):(150 to 400); mixing the debittered pomelo sac powder with rhizoma dioscoreae powder and fructus amomi powder according to the ratio of mass fraction being (80 to 100):(40 to 60):(30 to 50), and preparing a mixture into a crude product by techniques such as fermenting; after fermenting, blending the crude product with honey according to the mass ratio being (80 to 100):(2.5 to 10), bottling a mixture, and sterilizing the mixture to obtain a finished product. The honey pomelo sac is used as a raw material, so that the waste of honey pomelo surface skin with abundant essential oil components is avoided, and an added value of the honey pomelo sac is improved. The prepared honey pomelo sac stomach nourishing and fermenting beverage has no other additives, has certain stomach nourishing efficacy, and is a health-care beverage with higher health-care value.