9 results about "Aspergillus aculeatus" patented technology

The invention provides application of aspergillus aculeatus in fermentation processing of traditional Chinese medicine indigo naturalis. The invention further provides application of the combined use of the pediococcus pentosaceus and the aspergillus aculeatus in fermentation processing of the traditional Chinese medicine indigo naturalis. The combination of the aspergillus aculeatus and the pediococcus pentosaceus has obvious advantages in the fermentation of the indigo naturalis. The invention also provides a preparation method of indigo naturalis. In the aspect of an enzyme system, beta-glucosidase generated by the aspergillus aculeatus and the pediococcus pentosaceus can promote dissolution of an indigo precursor substance indole glycoside. In addition, other specific enzymes, such as glucoside hydrolase or oxidoreductase and the like, can also be generated, complex components in indigo naturalis are further decomposed through mutual cooperation of the two enzymes, more precursor substances are released, generation and accumulation of indigo blue and indirubin are jointly promoted, and the content of indigo blue and indirubin is increased. In addition, growth and metabolism of harmful microorganisms can be inhibited, harmful substances possibly generated in the indigo naturalis fermentation process are reduced, and the quality and safety of indigo naturalis products are improved.

The application discloses a tobacco endophytic fungus metabolite, a preparation method and application in tobacco alternaria alternata prevention and treatment, and relates to the technical field of microbial natural product pesticides. The tobacco endophytic fungus metabolite is a benzoquinone compound peniquinone L, and a structural formula is as follows: The benzoquinone compound peniquinone L is produced by fermentation of tobacco endophytic fungus Aspergillus aculeatus Aspergillus aculeatus Aa-1, and scale fermentation and preparation production are easy to carry out. The tobacco endophytic fungus metabolite benzoquinone compound peniquinone L of the application has inhibitory activity on various agricultural pathogens, and has significant bacteriostatic activity on tobacco alternaria alternata in particular, with an MIC value of 2 μg / mL, which is superior to positive drug carbendazim, and can be used as a leading compound or a new microbial natural product pesticide for tobacco alternaria alternata prevention and treatment.

This invention belongs to the field of food enzyme engineering technology, specifically relating to a strain of *Aspergillus echinococcosis* that produces highly efficient ester-degrading catechin tannin enzymes and its applications. The strain is classified as *Aspergillus echinococcosis* (…). Aspergillus aculeatus LS06, with accession number CGMCC No. 42170. The *Aspergillus echinococcosis* strain provided by this invention serves as a tannin-producing strain, using inexpensive agricultural waste bran as raw material, resulting in low production costs. Simultaneously, the produced tannins exhibit high enzyme activity and high catalytic efficiency for ester-type catechins, demonstrating promising application prospects in tea beverage processing.

The present invention discloses an Aspergillus aculeatus and its application in the production of tannase and gallic acid, belonging to the field of microorganisms and tannin degradation technology. The microorganism is classified and named Aspergillus aculeatus WYT2101, which has been deposited in the Guangdong Provincial Microbial Culture Collection Center with a deposit number of GDMCC No. 62691, a deposit date of August 10, 2022, and a deposit address of 5th Floor, Building 59, Institute of Microbiology, Guangdong Academy of Sciences. The Aspergillus aculeatus can use its tannase gene to prepare tannase with high enzyme activity (the tannase activity of the fermentation supernatant can reach 44.45U / mL) and good thermal stability; it can also use pomegranate peel to efficiently produce tannase (2.14U / mL) and gallic acid (the output reaches 1.7mg / mL), and has the potential to be applied to industrial mass production of tannase and gallic acid.

The invention provides a phenolic acid degradation fertilizer and a preparation method thereof, raw materials comprise metal cross-linked gel and engineering bacterium spores coated with the metal cross-linked gel, the engineering bacterium spores are one or more of white-rot fungus spores, trichoderma harzianum spores and aspergillus aculeatus spores, and the metal cross-linked gel comprises ferric chloride, sodium alginate and gelatin. Iron chloride, sodium alginate and gelatin form metal cross-linked gel to coat the engineering bacterium spores, phenolic acid pollutants in the environment can chelate metal ions in the metal cross-linked gel, and the engineering bacterium spores in the phenolic acid degradation fertilizer can be released only when the concentration of phenolic acid in the environment reaches a certain threshold value. Further, phenolic acid in the environment is decomposed, and it is ensured that all activity of functional bacteria is delivered to a phenolic acid enriched rhizosphere pollution area.

The invention provides a preparation method of a microbial combined modifier for cadmium-polluted rice soil remediation, which comprises the following steps: carrying out swelling treatment on a porous carrier material, and carrying out attenuation or disinfection treatment by using growth promoting liquid; the growth promoting liquid is a root exudate solution of cynodon dactylon, rice, ryegrass or rape; adding a porous carrier material and a growth promoting solution into the aspergillus aculeatus solution, and oscillating to obtain a mixed solution; and mixing the mixed solution with sodium alginate, and carrying out gelation treatment to obtain the microorganism combined modifier. The microorganism combined modifier overcomes the defects of biotoxicity, agglomeration and the like of a porous carrier material, solves the problems of low survival rate, poor tolerance and the like in a functional microorganism repairing process, increases the content of crop rhizosphere microorganism source growth-promoting hormone, effectively promotes the yield and stress resistance of rice, and improves the quality of the rice. Meanwhile, migration and bioavailability of rice rhizosphere heavy metal are effectively reduced, and absorption of rice to heavy metal such as cadmium is inhibited.

The invention belongs to the technical field of food enzyme engineering, and particularly relates to aspergillus aculeatus for producing efficient degradation ester type catechin tannase and application of the aspergillus aculeatus. The invention relates to aspergillus aculeatus, which is classified and named as aspergillus aculeatus LS06, and the preservation number of the aspergillus aculeatus is CGMCC (China General Microbiological Culture Collection Center) No.42170. The aspergillus aculeatus provided by the invention is used as a strain for producing tannase, and cheap agricultural product waste bran is used as a raw material, so that the production cost is low. Meanwhile, the produced tannase is high in enzyme activity, has very high catalytic efficiency on ester type catechin, and has the advantages of better application prospect in tea beverage processing and the like.

The preparation method of the fermented feed comprises the following steps: treating bagasse with an acetic acid solution to obtain a to-be-fermented solution, uniformly mixing the to-be-fermented solution, a nitrogen source, a carbon source and the compound microbial fermentation inoculant, and fermenting to obtain the fermented feed, the nitrogen source comprises corn flour, wheat bran and soybean meal; the compound microbial fermentation agent comprises aspergillus aculeatus, pichia pastoris and bacillus amyloliquefaciens. Compared with the prior art, the bagasse is crushed and then fermented with the soybean meal and the wheat to obtain the compound fermentation product, the compound fermentation product, the corn flour and the auxiliary materials are mixed and granulated to obtain the fermented feed, and the fermented feed is low in beta-glucan content, soybean agglutinin content and phytic acid content and good in feed conversion efficiency.

The present invention relates to the field of microbial fermentation technology, and discloses Aspergillus aculeatus GA2-37 and its application in Morchella polysaccharide extraction. The present invention provides a new microbial strain Aspergillus aculeatus GA2-37, and makes Aspergillus aculeatus GA2-37 grow appropriately in Morchella powder added with sucrose, produces multiple hydrolases, and then, the fermented Morchella powder is water-insulated, and substances such as cellulose in the cell wall are hydrolyzed, which helps the bound Morchella polysaccharide to be dissolved during ultrasonic water extraction, thereby significantly improving the extraction yield of polysaccharides. The present invention applies Aspergillus aculeatus GA2-37 to Morchella polysaccharide extraction, and the polysaccharide extraction yield can be increased by 36.4% compared with the conventional method of water extraction and alcohol precipitation.