Strain for producing ratgalacturonorhamnan hydrolase at high yield

A technology of galacturonic acid and rhamnose half, which is applied in the field of mutant strains of Aspergillus aculeatus, can solve the problems of few strains, unfavorable wide application, low yield and the like, and achieves the effects of reducing production costs and promoting popularization and application.
CN107988086AActive Publication Date: 2018-05-04QINGDAO VLAND BIOTECH GRP +1
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
QINGDAO VLAND BIOTECH GRP
Publication Date
2018-05-04
Patent Text Reader

Abstract

The invention belongs to the technical field of microbial transformation and particularly relates to an Aspergiulls aculeatus mutant strain for producing ratgalacturonorhamnan hydrolase at high yield.According to the Aspergills aculeatus mutant strain disclosed by the invention, Aspergiulls aculeatus DSM2344 is used as an original strain and an ultraviolet mutagenesis method is adopted to selectfor obtaining a mutant strain Aspergiullus aculeatus T13 with a preservation number of CCTCC NO: M2017784. After a mutant strain solid is subjected to solid shake flask fermentation for 3.5 days, theenzyme activity of the ratgalacturonorhamnan hydrolase reaches 63.7u / g, which is increased by 41.6 percent compared with that of an original strain; unexpected technical effects are achieved. The Aspergiulls aculeatus mutant strain provided by the invention can be widely applied to fermentation production of the ratgalacturonorhamnan hydrolase; the production cost of the enzyme is favorably reduced and promotion and application of the ratgalacturonorhamnan hydrolase in the food industry are promoted.
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Description

technical field

[0001] The invention belongs to the technical field of microbial transformation, and specifically relates to a mutant strain of Aspergillus aculeatus with high production rate of rhamnogalacturonate hydrolase. technical background

[0002] The cell walls of higher plants are composed of cellulose, an intersupporting structure formed mainly of hemicellulose polymers, pectin matrix polymers, and spherical and non-spherical proteins. With the exception of globular proteins, all polymers play structural roles in the cell wall. As the cell wall matures, cross-links, interchain and intrachain connections are formed, and finally lignin is deposited on the cell wall, thereby making the cell wall very stable and making its components difficult to separate, digest, and handle.

[0003] Pectin is a complex group of branched heteropolysaccharides that is abundant in the primary cell wall and in the interlayer between all plant cells. Pectin polymers are chemically dist...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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