The utility model discloses a
primary cell culture device, including the culture box, the culture box top is equipped with the top cover, is equipped with the injection
pipe fixedly on the top cover, the injection
pipe top is provided with sealing mechanism, and the sealing mechanism includes the adjusting sleeve and the sealing sleeve, and the adjusting sleeve is fixed at the injection
pipe top, and the sealing sleeve rotationally connected in the adjusting sleeve top, and the adjusting sleeve bottom surface abuts and is equipped with the sealing plate, the sealing plate top is fixedly equipped with the sliding block, and the sliding block top surface is fixedly equipped with the screw rod, the screw rod outer wall is equipped with the transmission sleeve of screw connection, and the transmission sleeve outside is fixedly equipped with the sliding sleeve, and the sliding sleeve and sealing sleeve inner wall slidingly connect, and the transmission sleeve bottom surface is equipped with the compression spring of connection, the sealing sleeve top is fixedly equipped with the connecting pipe, and the connecting pipe is equipped with the
syringe of screw connection on, through the delicate cooperation of adjusting sleeve and sealing sleeve and the collaborative work of sealing plate and sliding block, realized the automatic sealing and opening function in the material injection process.