1206 results about "Key genes" patented technology

The invention relates to a method and a system for constructing a dynamic gene regulation network, computer equipment, a computer readable storage medium, a computer program product and a computer program. The method comprises the following steps: constructing a plurality of single cell quasi-time sequence differentiation trajectories based on single cell transcriptome data of a plurality of single cells; determining a dynamic change gene in a target quasi-timing sequence differentiation trajectory based on the plurality of single cell quasi-timing sequence differentiation trajectories; constructing an initial static gene interaction network based on the dynamic change gene; and performing inspection based on the initial static gene interaction network to obtain the dynamic gene regulation network. According to the method, key gene regulation mechanisms corresponding to different differentiation stages of the cells can be rapidly determined, and the real dynamic change condition of the gene regulation relationship in the biological process is reflected.

The invention discloses a graph convolution spatial domain identification method of a fusion gene interaction network, and relates to the technical field of biological information, and the method comprises the steps: preprocessing spatial transcriptome data; constructing a spatial diagram according to the spatial position information of the spot and the gene expression matrix; constructing a conditional gene interaction network based on at least a part of key genes in the spatial transcriptome data, constructing a conditional gene interaction network matrix, combining the conditional gene interaction network matrix and the gene expression matrix to obtain an expression degree-interaction matrix, and constructing a feature map; performing graph neural network convolution on the space graph and the feature graph, and introducing an attention mechanism to obtain final embedding; and clustering and finally embedding, and identifying a spatial domain with a similar gene expression mode. According to the method, the spatial domain is identified through the fusion gene interaction network, the problem that the spatial domain is discontinuous is effectively solved, the robustness and accuracy of the spatial domain identification method are improved, and high performance is shown in complex and highly heterogeneous tissue samples.

The invention belongs to the technical field of biology, and relates to an escherichia coli engineering strain for producing L-isoleucine through a methyl malic acid way as well as a construction method and application of the escherichia coli engineering strain. According to the invention, Escherichia coli BW25113 is used as a chassis strain, and a methyl malic acid synthase high-activity mutant gene cimA3.7, an isopropyl malic acid isomerase gene GsleuCD, a 3-isopropyl malic acid dehydrogenase gene AfleuB and a leucine dehydrogenase gene Lsleudh are over-expressed; a methyl malic acid absorption protein gene is over-expressed, and a branched chain amino acid transport system is modified to improve the exosome of the L-isoleucine; all methyl malic acid pathway related genes are integrated to a genome of a chassis strain, and a plasmid-free L-isoleucine production strain is constructed; the copy number of a key gene on a genome is optimized, and the yield of the engineering strain L-isoleucine is increased by introducing a non-oxidative glycolysis approach. The yield of the L-isoleucine after the engineering strain is fermented for 34 hours in a fermentation tank is 56.6 g / L.

The invention discloses a sugarcane germplasm resource evaluation and breeding method for smart agriculture. The sugarcane germplasm resource evaluation and breeding method comprises the following steps: step 1, collecting materials with wide genetic diversity; establishing an intelligent incubator and a greenhouse, and monitoring environmental parameters by using an Internet of Things sensor; 2, performing data acquisition on the related germplasm resources by using an unmanned aerial vehicle, a robot and a near infrared spectrum technology, and constructing a phenotype database for data management; meanwhile, genotype identification is carried out by combining molecular marker-assisted selection and genome selection technologies, molecular markers of key genes are mined, and the genetic value of related germplasm is evaluated; step 3, constructing a hybrid combination prediction model by using an AI algorithm, optimizing parent matching of the related germplasm and the cultivated sugarcane, monitoring and collecting the filial generation at the same time, and analyzing and screening in combination with smart agriculture; 4, demonstration planting is conducted on the bred excellent sugarcane strain, and application of the excellent strain is improved through government-enterprise-scientific research institution collaborative popularization and in combination with farmer technical training.

The invention discloses a biosynthesis system for high-yield 1, 6-hexamethylenediamine and application of the biosynthesis system, and belongs to the technical field of biology. According to the method, key genes which are excavated and optimized in a 1, 6-hexamethylenediamine biosynthetic pathway are co-expressed in a single cell module or a multi-cell module, and different enzyme elements expressed by the cell module are subjected to enzyme cascade reaction, so that 1, 6-hexamethylenediamine can be efficiently synthesized by taking caprolactam or 6-aminocaproic acid as a substrate; furthermore, after the carboxylic acid reductase MaCAR is mutated, the catalytic activity of the carboxylic acid reductase MaCAR can be remarkably improved, and the yield of the 1, 6-hexamethylenediamine is further improved; in addition, the biosynthesis system disclosed by the invention directly takes the cell module as a whole-cell catalyst, and an enzyme purification step is avoided, so that the biosynthesis system has the advantage of low cost.

According to the laodelphax striatellus ecdysis gene LsMH and the application thereof in biological prevention and control of laodelphax striatellus, the laodelphax striatellus ecdysis gene LsMH serves as one of key genes for ecdysis of laodelphax striatellus, the laodelphax striatellus ecdysis gene LsMH can be subjected to targeted interference, laodelphax striatellus can be remarkably inhibited from forming adults through eclosion, and laodelphax striatellus death is avoided, so that laodelphax striatellus population is the harm of the laodelphax striatellus to food crops is reduced. The control of laodelphax striatellus is carried out through a biological means, the method is safe and harmless to environment ecology and food, and a new strategy is provided for green control of laodelphax striatellus. The dsRNA or the interference reagent is prepared through transcription of the laodelphax striatellus ecdysis gene LsMH, the LsMH gene can be effectively interfered after the dsRNA or the interference reagent is injected into the laodelphax striatellus, the phenomena that wings are bent or incomplete, the abdomen is wizened and ecdysis is incomplete occur in the ecdysis process of laodelphax striatellus nymphs, laodelphax striatellus eclosion into adults is inhibited, laodelphax striatellus death is avoided, and biological control over laodelphax striatellus is achieved.

The invention discloses a tomato fruit cracking key regulation gene identification method, through the method, 41 key genes for regulating tomato cracking are determined and obtained for the first time, and the genes mainly participate in plant hormone synthesis, cell wall metabolism, wax and cuticle metabolism, absorption and transportation of mineral elements and water, and some transcription factors. Two regulation networks are established and comprise an ethylene-cell wall metabolic pathway and a transcription factor-water channel-mineral element. The research combines high-throughput sequencing and molecular biological techniques, enriches the cracking mechanism of tomato fruits on transcription and translation levels, and thus, has important scientific significance and application prospects.

The invention is applicable to the technical field of bioengineering, and provides a coding gene for enhancing iron deficiency stress tolerance of plants and application. According to the soybean GmMYB14 transcription factor disclosed by the invention, the problem that the growth of soybeans is limited in an iron-deficient environment, particularly calcareous soil and soda saline alkali soil, is solved by excavating and applying the encoding gene (the nucleotide sequence is shown as SEQ ID No.1) of the soybean GmMYB14 transcription factor. Experiments prove that after the gene is over-expressed in arabidopsis thaliana and soybean, the tolerance of plants to iron deficiency stress can be remarkably enhanced. The invention provides a key gene resource for molecular breeding: the GmMYB14 effectively promotes the absorption and utilization of plants on iron by regulating and controlling the metabolic pathway of phenylpropane, not only can improve the planting adaptability of soybeans in low-iron soil, but also can improve the yield and quality of the soybeans, provides powerful technical support for enlarging the planting area of the soybeans in iron-deficient soil regions, and has a wide application prospect. The method is of great significance in relieving insufficient soybean productivity in China and improving the self-sufficiency rate.

The invention provides a 5-hydroxytryptamine Escherichia coli production strain and a construction method and application thereof, the strain is deleted with tnaA, pykA, trpR, ldhA and trpL genes, trpEfbr, aroGfbr, serAfbr, tktA and prsA genes are up-regulated, gdh and glnAfbr genes are heterologously expressed, a pykF gene is dynamically regulated and controlled, and meanwhile, the production strain carries a plasmid pACYC-FYSPD and a plasmid pETDuet-TM2-RBS-AnTDC; the constructed strain has the characteristic of metabolic balance, the expression levels of a tryptophan hydroxylation-decarboxylation system and a coenzyme tetrahydrobiopterin synthesis regeneration system are adjusted by optimizing the expression intensity of key genes of each precursor and simultaneously by virtue of a double-plasmid system, and 5-hydroxytryptamine can be efficiently synthesized in a culture medium without byproducts.

The invention relates to a mulberry MaBEH1 / 2 gene for improving plant salt tolerance and application thereof, and belongs to the technical field of plant genetic engineering and biology. Wherein the coding nucleotide sequence of the mulberry MaBEH1 / 2 gene for regulating and controlling the salt tolerance of the woody plant is shown as a sequence 3; and the amino acid sequence of the MaBEH1 / 2 expression protein is shown as a sequence 4. According to the invention, MaBEH1 / 2 is transferred into 84K poplars to obtain MaBEH1 / 2-OE transgenic poplars; compared with a non-transgenic poplar, the salt stress tolerance of the MaBEH1 / 2-OE transgenic poplar can be remarkably enhanced. The invention provides key gene resources and technical support for genetic improvement of salt-tolerant forest varieties.

The invention discloses a biological complex trait key gene identification method, device and equipment and a medium, and the method comprises the following steps: obtaining biological reference genome data, and carrying out orthologous gene class group division on a protein sequence coded by the biological reference genome data; determining a characteristic value data set according to the number of the subgroup sequence and the gene number of the subgroup sequence obtained after division; obtaining each piece of biological phenotype data; utilizing a machine algorithm model to construct a phenotype matrix according to the biological phenotype data and the characteristic value data set so as to train a phenotype prediction model, and determining the influence weight of each subgroup sequence on the biological phenotype according to the phenotype prediction model; and according to each influence weight, determining a key gene for regulating and controlling the biological complexity. The method provided by the invention can realize more accurate, efficient and comprehensive identification of key genes of prokaryotes and high biological complexity characters.

The invention provides a beauveria bassiana engineering strain Bb-dsMucin for expressing dsRNA (double-stranded ribonucleic acid) as well as a construction method and application of the beauveria bassiana engineering strain Bb-dsMucin. The beauveria bassiana is a beauveria bassiana QSE-F1 strain, and the strain can be colonized at the tomato leaves. The dsRNA can target a botrytis cinerea key gene BcMucin, and the expression of the BcMucin gene is silenced in an RNAi (Ribonucleic Acid Interference) mode. According to the engineering strain, a carrier containing a target gene sequence is transferred into a beauveria bassiana QSE-F1 strain by utilizing an ATMT technology, and the engineering strain Bb-dsMucin capable of synthesizing dsRNA of targeted BcMucin is obtained. The invention also provides an application of the engineering strain Bb-dsMucin, and the application is that the engineering strain Bb-dsMucin can interfere with the expression of a target gene BcMucin, so that the botrytis cinerea can be effectively prevented and treated. Experiments prove that the provided engineering strain Bb-dsMucin can be used for preparing biological agents for preventing and treating gray mold and has a good market application prospect.

The invention discloses development and application of a molecular marker of a rape stain tolerance related gene BnaERF3, and belongs to the technical field of molecular biology. According to the invention, the BnaA06. ERF3 gene is screened on the basis of whole genome associated molecules, and is used as a key gene in QTL (qWL.A06) to participate in regulation and control of the waterlogging tolerance of the rape. A molecular marker related to the waterlogging tolerance of the brassica napus is developed aiming at the BnaA06. ERF3 gene, and tests show that the waterlogging tolerance of the brassica napus can be detected by utilizing the molecular marker, so that varieties containing waterlogging tolerance genes are subjected to auxiliary selection and breeding, and the waterlogging tolerance breeding process of the brassica napus is accelerated.

The invention discloses a key gene identification method related to tobacco nitrogen response, which comprises the following steps: S1, setting four nitrogen fertilizer gradient treatments on the basis of same phosphorus and potassium fertilization by adopting a field experiment of a completely random block; s2, randomly taking the 6th to 8th leaves of the five plants in each area, and dividing a sample into two parts: quickly freezing one part with liquid nitrogen, and storing at-80 DEG C for RNA (Ribonucleic Acid) extraction and transcriptome sequencing; one part is used for measuring the nitrogen content, and after baking and drying treatment, a KjeltecTM8100 automatic nitrogen analyzer is used for measuring; s3, nitrogen content determination: determining the nitrogen content in the treated sample by using an automatic nitrogen analyzer KjeltecTM8100; according to the method, high-throughput transcriptome sequencing and weighted gene co-expression network analysis (WGCNA) are combined, so that not only can gene expression maps of flue-cured tobacco leaves treated by different nitrogen fertilizers be comprehensively captured, but also gene modules with similar expression modes can be mined from a global perspective.

The invention discloses a rice drought resistance phenotype detection and genetic analysis method based on a phenotypic robot, which is characterized in that rice hyperspectral phenotypic character data is acquired based on the phenotypic robot, and the relative water content RWC of rice is predicted. Through high-frequency data acquisition, RWC (t) based on a time sequence is obtained, RWC (t) curve change characteristic indexes are calculated, the dynamic change process of a rice experiment material in the whole drought experiment period is quantified, the drought resistance index DTI of the rice experiment material is obtained, and the drought resistance of the rice variety is comprehensively evaluated. DTI as a user-defined phenotypic character can be used for whole genome association analysis (GWAS), and a key gene for controlling the drought resistance of rice is excavated. According to the invention, the problems of low efficiency and low flux of traditional rice phenotype detection are solved, phenotype-gene mining based on the mobile robot is realized, and a new way is expanded for improvement of rice genetic breeding.

The invention discloses a GmbZIP36 protein related to aluminum resistance of plants in acid soil as well as a coding gene and application of the GmbZIP36 protein, and belongs to the technical field of bioengineering. The amino acid sequence of the GmbZIP36 protein is shown as SEQ ID NO: 2, 3 or 4, and the nucleotide sequence of the coding gene of the GmbZIP36 protein is shown as SEQ ID NO: 1. The invention finds that the expression of the GmbZIP36 gene is induced by aluminum stress, the relative root elongation and root biomass of arabidopsis thaliana and soybean under the aluminum stress can be obviously improved by over-expressing the gene in a plant, and aluminum accumulation is reduced, so that the aluminum resistance of the plant is enhanced. The invention provides a recombinant vector containing the gene, a host cell and application of the recombinant vector and the host cell in regulating and controlling the aluminum resistance of plants. Meanwhile, the invention also provides a method for producing an aluminum-resistant transgenic plant by using the gene, a kit for detecting the gene or protein and a method for carrying out assisted breeding by using the molecular marker linked with the gene. The invention provides a key gene resource for aluminum-resistant molecular breeding of crops, and has important significance for cultivating new varieties of crops adapting to acid soil.

The invention discloses a method for identifying key mitochondrial autophagy and ferroptosis genes in sepsis-induced ARDS and an immunoregulation mechanism of the key mitochondrial autophagy and ferroptosis genes. The method comprises the following steps: screening differential expression genes DEGs related to the sepsis-induced ARDS from a GEO gene expression database; and screening the DEGs by using LASSO and SVM + RFE, and identifying 12 key DEGs. The diagnostic potential of the genes in sepsis-induced ARDS is verified through an ROC curve, and the effect of the genes in immune response is disclosed through gene enrichment analysis and immune infiltration analysis. According to the invention, a key gene FTH1 is identified, a miRNA gene [miR-224-5p] targeting the FTH1 and a pathologic process in which the miRNA gene [miR-224-5p] participates in regulation and control of sepsis-induced ARDS are found, and a close relationship between the FTH1 and E3 ubiquitin ligase SMURF1 is found.

The invention relates to the technical field of tumor data processing, in particular to a methylation-based tumor data processing system. The system comprises the following modules: a methylated sample sequencing module, a sequencing difference site recognition module, a tumor gene sequence analysis module and a tumor subtype classification module, performing high-throughput sequencing on the to-be-detected clinical tumor DNA sample to obtain methylated tumor sequencing data; performing methylation level quantification on the methylated tumor sequencing data, and performing tumor difference site analysis to generate tumor difference site data; performing tumor generation key gene sequence identification according to the tumor difference site data to generate tumor methylation characteristic data; and performing tumor subtype tag identification according to the tumor methylation characteristic data to generate tumor type tag data. According to the method, accurate tumor typing is realized on the basis of tumor DNA methylation characteristic analysis, and the false negative rate of low-concentration sample detection is effectively reduced.

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The invention relates to the technical field of cotton alkali-resistant gene mining, and particularly discloses a method for jointly screening key genes responding to upland cotton alkali stress based on transcriptome and metabolome, which comprises the following steps: carrying out alkali stress treatment on upland cotton to obtain tissue samples at different time points; performing transcriptome sequencing and metabolome sequencing on the tissue sample to respectively obtain gene expression data and metabolite data; performing difference analysis on the gene expression data and the metabolite data to obtain DEGs and DAMs; carrying out conjoint analysis on the DEGs and the DAMs, and screening to obtain a key gene and a regulatory pathway which respond to alkali stress; analyzing the key gene and the regulatory pathway through a gene expression verification technology to obtain an expression mode; according to the invention, 15 candidate genes related to the alkali resistance of the upland cotton are screened through WGCNA, a theoretical basis is provided for deeply knowing the molecular mechanism of the alkali resistance of the upland cotton, and a new gene resource is provided for subsequent research.

The invention discloses a sorghum aphid MsCYP1 gene as well as an encoding protein, dsRNA (double-stranded ribonucleic acid) and application thereof, and aims to solve the technical problem that a key gene is lacked when an RNA (ribonucleic acid) interference technology is used for biological prevention and control of sorghum aphids at present. According to the application, the sorghum aphid protein MsCYP1 capable of being secreted into sorghum cells is screened, and the protein and the coding gene thereof can inhibit the content of hydrogen peroxide in sorghum and the immune response of the sorghum. Meanwhile, according to the cDNA sequence of the MsCYP1 gene of the sorghum aphid, dsRNA for the gene is designed, the dsRNA is introduced into the body of the sorghum aphid in an artificial feeding mode, it is found that the dsRNA can remarkably inhibit the MsCYP1 gene of the sorghum aphid, then the honeydew secretion amount and the reproduction rate of the sorghum aphid are affected, the MsCYP1 gene can serve as an RNAi target gene to be used for aphid prevention and control, pesticide use is reduced, and the application prospect is good. The ecological balance is maintained.

The invention relates to the technical field of biology, and particularly discloses application of a PagYUCCA6a gene in regulation and control of poplar wood yield. The PagYUCCA6a gene is cloned by taking a poplar variety 84K as a material and is constructed to an overexpression vector pMDC32, the gene is positioned behind a 35S promoter, and under the driving of the 35S promoter, the PagYUCCA6a can be overexpressed in a poplar body, so that the poplar wood yield is regulated and controlled, the PagYUCCA6a gene is a key gene for regulating and controlling the poplar wood yield, and the PagYUCCA6a gene can be used for regulating and controlling the poplar wood yield. The method has important application value in the field of forest high-yield gene engineering.

The invention discloses application of a transcription factor NtMYC2 as well as an encoding protein and an expression vector thereof in plant epidermal hair regulation and control, and aims to solve the technical problems that a plant epidermal hair generation mechanism is unknown and an effective regulation and control means and a utilization way are lacked at present. A transcription factor NtMYC2 capable of regulating and controlling plant epidermal hair generation is identified, the gene is located in a cell nucleus, the expression level in leaves is the highest, the gene is a key component in a jasmonic acid signal channel, the expression of the gene is regulated and controlled by a jasmonic acid signal, and the gene can be used for regulating and controlling plant epidermal hair generation. By constructing a gene editing strain of the gene, it is clear that NtMYC2 serves as a key gene to participate in positive regulation of epidermal hair generation, and the NtMYC2 has important application value in the aspect of regulating the total epidermal hair development density.

The invention discloses an acute myocardial infarction prognosis prediction model establishment method and system, and relates to the technical field of risk prediction.The method comprises the steps that patient chip data is collected and screened, and a first myocardial infarction key gene is obtained; verifying the first myocardial infarction key gene to obtain a second myocardial infarction key gene; analyzing the relationship between the expression level of the key gene and the prognosis of the patient based on the second myocardial infarction key gene; and constructing an acute myocardial infarction prognosis prediction model by using the relationship between the expression level of the key gene and the prognosis of the patient, and performing performance evaluation. By combining immune-related LncRNA, a second myocardial infarction key gene is screened out, an acute myocardial infarction prognosis prediction model is constructed, the occurrence rate of MACE events of a patient is effectively predicted, the accuracy of myocardial infarction prognosis prediction is improved, high-risk patients are screened, and more effective prevention and treatment are provided.

The invention provides dsRNA (double-stranded ribonucleic acid) targeting a BcMucin gene and application of the dsRNA in preparation of a preparation for preventing and treating gray mold. The dsRNA is obtained by taking a coding sequence of a target gene BcMucin as a template through manual design and in-vitro synthesis. The dsRNA with the BcMucin as the target can be used for effectively inhibiting the occurrence of the botrytis cinerea. Experiments prove that after the deletion of the target gene BcMucin provided by the invention, botrytis cinerea hyphae grow slowly, the pathogenicity to tomatoes is completely lost, and the target gene BcMucin is a key gene for botrytis cinerea growth and pathogenicity regulation. The dsRNA provided by the invention can effectively interfere the expression of a target gene BcMucin in botrytis cinerea so as to inhibit the growth and development of the botrytis cinerea and the infection of the botrytis cinerea on host plants and reduce the biomass of the botrytis cinerea on scabs. The dsRNA provided by the invention can be used for RNA pesticide development based on RNAi, is applied to effective prevention and treatment of gray mold, and has a good market application prospect.

The invention relates to cloning of a stress-resistant gene LpbHLH75 of Lilium pumilum DC (Lilium pumilum DC.) and application of the stress-resistant gene LpbHLH75 of Lilium pumilum DC. The lilium tenuifolium stress resistance key genes are mined through transcriptome data analysis, and the functions of the lilium tenuifolium stress resistance key genes are studied. Comprising the following steps: (1) cloning a target gene; (2) constructing a plant overexpression vector; and (3) detecting physiological indexes of the transgenic plant. According to the experiment, lilium tenuifolium is taken as a research object, and the LpbHLH75 gene is cloned by applying an RT-PCR technology; transforming tobacco by constructing an overexpression vector; the function of overexpression of the LpbHLH75 on tobacco salt and alkali and drought stress is identified through transgenic tobacco, the function of the lilium tenuifolium bHLH gene under adversity stress is disclosed, a new gene source is provided for lilium tenuifolium genetic engineering breeding, a reference is provided for later lilium tenuifolium stress-resistant germplasm resource research, and the lilium tenuifolium bHLH gene is developed. And a foundation is laid for exploring a gene capable of enhancing stress resistance in the lilium tenuifolium and a stress resistance mechanism of the gene.

The invention belongs to the technical field of strain metabolism modification, and particularly relates to a streptomyces avermitilis strong promoter and application. The nucleotide sequence of the streptomyces avermitilis strong promoter provided by the invention is as shown in SEQ ID NO. 4. The strong promoter can express and regulate a key gene, and the mRNA relative expression level of the gene driven by the strong promoter can still be kept stable in the 48-96-hour growth stage of the streptomyces avermitilis, which indicates that the strong promoter can keep high activity in the 48-96-hour growth stage of the streptomyces avermitilis, so that the target gene can be stably and highly expressed. Therefore, the strong promoter can be used for constructing recombinant plasmids for expressing key genes for synthesizing target natural products and streptomyces avermitilis recombinant bacteria, and the constructed streptomyces avermitilis recombinant bacteria can efficiently produce the target natural products.

The invention relates to a key gene for duck meat and egg character differentiation and a method for excavating the key gene based on whole genome sequencing, the key gene is a duck SIK1 gene, and the nucleotide sequence of the key gene is shown as SEQ ID NO: 1. The key gene SIK1 influencing the differentiation and domestication process of laying ducks and meat ducks is found by using a method for analyzing the key gene in the domestication process of duck meat traits and egg traits through whole genome re-sequencing. The gene SIK1 plays a role in regulating glycometabolism and cell proliferation. The genome of the Jinding duck is assembled by using a whole genome re-sequencing technology for the first time and is compared with the genome of other duck species such as Beijing duck, another key gene SIK1 for meat and egg trait differentiation of ducks is excavated, molecular breeding of ducks is facilitated, and a new thought is provided for searching key genes for meat / egg duck trait differentiation in future.

The invention provides a tobacco polyphenol oxidase gene NtPPO15 and application of the tobacco polyphenol oxidase gene NtPPO15 in inhibiting browning of tobacco and improving ripening resistance and baking resistance of the tobacco. According to the invention, a key gene NtPPO15 related to tobacco browning and baking resistance is screened through experiments, the activity of an NtPPO15 promoter and a gene expression site are further explored by constructing a GUS dyeing vector, and the function of the gene is deeply studied through gene editing and overexpression. Experiments prove that the NtPPO15 gene provided by the invention is a key gene related to browning in a tobacco maturing and baking process, and by regulating and controlling the expression quantity of the NtPPO15 gene, the browning of the tobacco can be controlled, the maturing resistance and baking resistance of the tobacco can be improved, the quality of a tobacco product can be improved, the harvesting frequency can be reduced, and the economic benefit can be greatly improved; and the method can also be used for screening tobacco varieties with excellent quality, so that the method has a good and wide market application prospect.

The invention relates to the technical field of biology, in particular to a benzene-induced blood abnormality marker, an evaluation model and a construction method and application of the benzene-induced blood abnormality marker and the evaluation model, and provides the following scheme that the method comprises the steps that S1, principal component analysis is conducted on benzene damage expression profile data sets, then preliminary screening conditions are set, and differential expression genes between the benzene damage expression profile data sets are screened out; s2, carrying out GO, KEGG and GSEA enrichment analysis on the differentially expressed gene to obtain an analysis result; s3, different machine learning algorithms are used for screening characteristic genes from analysis results, intersection is taken, key genes are obtained, then verification analysis is conducted on the key genes, and benzene damage marker genes are obtained; and S4, training and testing a plurality of learning models by using the benzene injury marker gene, and selecting a proper learning model as a benzene-induced blood abnormality evaluation model according to a test result of the learning model. According to the invention, a theoretical basis can be provided for early intervention of benzene toxicity, and a more comprehensive and sensitive model can be provided for risk assessment.