631 results about "Key genes" patented technology

The invention discloses a biosynthesis system for high-yield 1, 6-hexamethylenediamine and application of the biosynthesis system, and belongs to the technical field of biology. According to the method, key genes which are excavated and optimized in a 1, 6-hexamethylenediamine biosynthetic pathway are co-expressed in a single cell module or a multi-cell module, and different enzyme elements expressed by the cell module are subjected to enzyme cascade reaction, so that 1, 6-hexamethylenediamine can be efficiently synthesized by taking caprolactam or 6-aminocaproic acid as a substrate; furthermore, after the carboxylic acid reductase MaCAR is mutated, the catalytic activity of the carboxylic acid reductase MaCAR can be remarkably improved, and the yield of the 1, 6-hexamethylenediamine is further improved; in addition, the biosynthesis system disclosed by the invention directly takes the cell module as a whole-cell catalyst, and an enzyme purification step is avoided, so that the biosynthesis system has the advantage of low cost.

The invention relates to a mulberry MaBEH1 / 2 gene for improving plant salt tolerance and application thereof, and belongs to the technical field of plant genetic engineering and biology. Wherein the coding nucleotide sequence of the mulberry MaBEH1 / 2 gene for regulating and controlling the salt tolerance of the woody plant is shown as a sequence 3; and the amino acid sequence of the MaBEH1 / 2 expression protein is shown as a sequence 4. According to the invention, MaBEH1 / 2 is transferred into 84K poplars to obtain MaBEH1 / 2-OE transgenic poplars; compared with a non-transgenic poplar, the salt stress tolerance of the MaBEH1 / 2-OE transgenic poplar can be remarkably enhanced. The invention provides key gene resources and technical support for genetic improvement of salt-tolerant forest varieties.

The invention discloses a key gene identification method related to tobacco nitrogen response, which comprises the following steps: S1, setting four nitrogen fertilizer gradient treatments on the basis of same phosphorus and potassium fertilization by adopting a field experiment of a completely random block; s2, randomly taking the 6th to 8th leaves of the five plants in each area, and dividing a sample into two parts: quickly freezing one part with liquid nitrogen, and storing at-80 DEG C for RNA (Ribonucleic Acid) extraction and transcriptome sequencing; one part is used for measuring the nitrogen content, and after baking and drying treatment, a KjeltecTM8100 automatic nitrogen analyzer is used for measuring; s3, nitrogen content determination: determining the nitrogen content in the treated sample by using an automatic nitrogen analyzer KjeltecTM8100; according to the method, high-throughput transcriptome sequencing and weighted gene co-expression network analysis (WGCNA) are combined, so that not only can gene expression maps of flue-cured tobacco leaves treated by different nitrogen fertilizers be comprehensively captured, but also gene modules with similar expression modes can be mined from a global perspective.

The invention discloses a rice drought resistance phenotype detection and genetic analysis method based on a phenotypic robot, which is characterized in that rice hyperspectral phenotypic character data is acquired based on the phenotypic robot, and the relative water content RWC of rice is predicted. Through high-frequency data acquisition, RWC (t) based on a time sequence is obtained, RWC (t) curve change characteristic indexes are calculated, the dynamic change process of a rice experiment material in the whole drought experiment period is quantified, the drought resistance index DTI of the rice experiment material is obtained, and the drought resistance of the rice variety is comprehensively evaluated. DTI as a user-defined phenotypic character can be used for whole genome association analysis (GWAS), and a key gene for controlling the drought resistance of rice is excavated. According to the invention, the problems of low efficiency and low flux of traditional rice phenotype detection are solved, phenotype-gene mining based on the mobile robot is realized, and a new way is expanded for improvement of rice genetic breeding.

The invention discloses a GmbZIP36 protein related to aluminum resistance of plants in acid soil as well as a coding gene and application of the GmbZIP36 protein, and belongs to the technical field of bioengineering. The amino acid sequence of the GmbZIP36 protein is shown as SEQ ID NO: 2, 3 or 4, and the nucleotide sequence of the coding gene of the GmbZIP36 protein is shown as SEQ ID NO: 1. The invention finds that the expression of the GmbZIP36 gene is induced by aluminum stress, the relative root elongation and root biomass of arabidopsis thaliana and soybean under the aluminum stress can be obviously improved by over-expressing the gene in a plant, and aluminum accumulation is reduced, so that the aluminum resistance of the plant is enhanced. The invention provides a recombinant vector containing the gene, a host cell and application of the recombinant vector and the host cell in regulating and controlling the aluminum resistance of plants. Meanwhile, the invention also provides a method for producing an aluminum-resistant transgenic plant by using the gene, a kit for detecting the gene or protein and a method for carrying out assisted breeding by using the molecular marker linked with the gene. The invention provides a key gene resource for aluminum-resistant molecular breeding of crops, and has important significance for cultivating new varieties of crops adapting to acid soil.

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The invention relates to a key gene GqBurscon for wing development of a Qinghai grassland moth and application thereof. The key gene GqBurscon for the wing development of the Qinghai grassland caterpillar has a nucleotide sequence as shown in SEQ ID NO: 1. According to the dsRNA synthesized by the key gene GqBurscon for wing development of the Qinghai grass caterpillar, one chain of the dsRNA is the same as the sequence of SEQ ID NO: 1, and the other chain of the dsRNA is reversely complementary with the sequence of SEQ ID NO: 1. The key gene GqBurscon for the wing development of the Qinghai grassland is used for synthesizing dsRNA, the synthesized dsRNA is used for carrying out RNA interference on a target gene, the gene transcription level is knocked down, the gene is effectively silenced in chest, abdomen, wings and wing base tissues of male adult Qinghai grassland, the length and width of the wings are correspondingly reduced, the development of the wings is limited, and the gene transcription level is reduced. The flying capability is influenced, and the death is caused.

The invention discloses a neural network calculation method and device for gene expression regulation and control analysis, and relates to the technical field of bioinformatics, and the method comprises the steps: obtaining first feature data and second feature data; constructing an input feature comprising a plurality of regulation and control hierarchies; and inputting the input features of the plurality of regulation levels and the second feature data into the target neural network model, and outputting a prediction result of the gene expression state. According to the neural network calculation method provided by the invention, chromatin accessibility and three-dimensional space interaction data are deeply fused through a dynamic routing module, so that the problem of'black box 'which is inaccurate in prediction and difficult to explain in a traditional deep learning model is solved in a mode of explicitly simulating a real biological regulation mechanism; and a key gene regulatory pathway can be accurately identified.

The invention belongs to the technical field of plant molecular biology, and discloses an application of a soybean GmSW10 gene in reducing soybean plant height, grain weight and grease content and / or increasing protein content. The CDS sequence of the soybean GmSW10 gene is as shown in SEQ ID NO: 1. Knockout of the soybean GmSW10 gene can directly influence the plant height, the grain weight, the oil content and the protein content of soybean seeds, after the soybean GmSW10 gene is knocked out, the weight of soybean seeds and hundred grains is remarkably reduced, the plant height is reduced, the protein content is increased, and the oil content is reduced, which indicates that the GmSW10 gene is a key gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean, and the GmSW10 gene can be used for preparing the soybean GmSW10 gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean. And a new genetic resource is provided for genetic improvement of soybeans.

The invention discloses a Pseudomonas putida high-yield rhamnolipid fermentation medium based on a step-by-step optimization strategy, an optimization method and application thereof, through the step-by-step strategy of component screening-single-factor optimization-multi-factor interactive optimization, a mixed carbon source and a mixed nitrogen source are taken as cores, and a Plackett-Burman test, a steepest climbing test and Box-Behnken response surface design are combined, so that the high-yield rhamnolipid fermentation medium for the pseudomonas putida high-yield rhamnolipid is obtained. Optimizing to obtain a special fermentation culture medium; two genetic engineering strains estA-2 and estA-3 are constructed by heterologous expression of key genes, and the supply and extracellular transport efficiency of a rhamnolipid synthesis precursor is enhanced; the recombinant strain is applied to the optimized culture medium, and efficient production of a shake flask and a 5L fermentation tank is achieved. The rhamnolipid yield of the estA-3 strain in a 5L fermentation tank reaches 35.59 + / -2.18 g / L, the conversion rate is 0.282 g / g, the productivity is 0.404 g / L / h, the rhamnolipid yield, the conversion rate and the productivity are obviously superior to those in the prior art, and a key technical support is provided for industrial production of rhamnolipid.

The invention belongs to the field of plant genetic engineering, and discloses a key gene OsHPCA1 for positively regulating salt stress and oxidative stress tolerance of rice. The gene (with the login number of Os05g40770) encodes a receptor protein capable of specifically sensing hydrogen peroxide, and belongs to a leucine repeat receptor-like protein kinase (LRR-RLKs) family, wherein the family is the largest subfamily in the receptor-like protein kinase (RLKs). The OsHPCA1 responds to salt and oxidative stress signals, and the salt tolerance and oxidative stress resistance of the rice are positively regulated and responded. The OsHPCA1 overexpression rice strain shows very strong salt tolerance; and the OsHPCA1 knockout strain oshpca1 is more sensitive to salt stress. The OsHPCA1 protein encoded by the OsHPCA1 gene and rich in leucine repeat receptor-like protein kinase can positively regulate and control the activity of catalase C (CatC) in rice and enhance the removal efficiency of H2O2 under salt stress, so that the salt tolerance of rice is improved. When the OsHPCA1 is overexpressed, the activity of CatC in the overexpressed strain OsHPCA1 is obviously increased, the H2O2 removal capability is enhanced, and the salt tolerance and oxidative stress tolerance of the rice are improved.

The invention discloses recombinant escherichia coli capable of producing salidroside and application, exogenous genes are introduced by utilizing a CRISPR / Cas9 technology, the recombinant escherichia coli capable of improving the fermentation yield of the salidroside is obtained by modifying and screening key genes, and finally, the salidroside is obtained by optimizing a fermentation tank process. The recombinant Escherichia coli can reach the highest yield of salidroside produced by fermentation of the scale at present on a 5L fermentation tank. The salidroside is fermented in a 5L fermentation tank for 104 hours, so that the maximum accumulation amount of the salidroside is 33.68 g / L, and industrial enlarged production is facilitated.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production through a UDPG synthesis route. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, the intracellular concentration of the UDPG is increased by systematically enhancing a UDPG synthesis pathway (knocking out ugd, glgc, ushA, agp, deoA, tdk, udp, or thrA, and overexpressing galU, pgm, pyrE, pyrH, ugpA, or prs), so that the production capacity of the stevioside is remarkably improved.

The invention discloses application of a gene GlZn2Cys661 in regulation and control of synthesis of ganoderic acid. The nucleotide sequence of the gene GlZn2Cys661 is as shown in SEQ ID NO. 1. The gene expression is regulated and controlled by constructing an overexpression or silent vector, so that the synthesis of the ganoderic acid is promoted or inhibited, and an effective method is provided for regulating and controlling the yield of the ganoderic acid. The invention relates to the technical field of bioengineering, and discloses an application of a gene GlZn2Cys661 in regulation and control of ganoderic acid synthesis. The invention provides a key gene target and a core engineering strain for efficiently producing ganoderic acid through a metabolic engineering means, and has important application value.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production by improving the utilization rate of a carbon source. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, by knocking out related genes and bypass metabolism genes (mgsA, iclR, menA or adhE) synthesized by organic acid, a glucose transport and utilization system (fnr, fadR, ppc, zwf or pgi is knocked out, and ACS, fbaA, tpiA, glf, galP or ptsG is overexpressed, so that the utilization rate of a carbon source is further improved, and the production capacity of stevioside is remarkably improved.

The invention belongs to the technical field of biosynthesis of plant secondary metabolites, and particularly relates to a key gene for biosynthesis of large-fruit hawthorn flavonoid compounds and a screening method and application of the key gene. Through combined analysis of metabolome and transcriptome, a key gene combination containing seven structural genes and three transcription factor genes is screened out; the expression of the genes is remarkably positively correlated with the accumulation of a target flavone metabolite [6]-gingerol, and the genes are core factors for regulating and controlling the synthesis of the flavonoid compounds of the big-fruit hawthorns through experimental verification. According to the invention, the key gene for regulating and controlling the synthesis of flavone substances such as [6]-gingerol in the big hawthorn fruit is systematically identified for the first time, and a target spot is provided for analyzing a quality formation mechanism from a molecular level; the gene can be used for molecular marker-assisted breeding so as to cultivate a new variety of large-fruit hawthorn with high flavone content, and also can provide gene resources and technical support for the development of functional food and health care products.

The invention discloses application of an overexpressed carp lpla gene in regulation and control of expression of cyprinid fish tissue-related genes, and relates to the field of gene engineering, in particular to application of the overexpressed carp lpla gene in regulation and control of cyprinid fish tissue-related genes. The overexpressed carp lpla gene is applied to regulation and control of contents of lipid indexes of total cholesterol TC and triglyceride TG of different tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulation and control of fat marker genes lpla, ppar gamma, Fabp3, fast and acaca of livers and muscle tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulating and controlling the relative expression quantity of carp liver cell fat metabolism related genes lpla, ppar gamma, Fabp3, fast, acaca, hsl and pnpla2. The overexpressed carp lpla gene can regulate and control lipid deposition in different tissues in zebra fish, and a research model is provided for lpla to regulate and control fat deposition and differentiation. According to the overexpressed carp lpla gene disclosed by the invention, lpla high-expression carp hepatocytes are obtained through a liposome transfection technology, so that fat key gene expression in the carp hepatocytes is changed.

The embodiment of the invention relates to a plant metabolite synthesis center and key gene analysis method and device. The method comprises the steps of constructing a partial differential equation, designing two neural network models, setting two ordinary differential equations, setting a data format of a sampling data sequence, designing a loss function LALL and designing a model training process. Intercepting experimental materials and storing the experimental materials; sampling and storing the frozen slices of the experimental material; after sampling is finished, setting sampling data sequences of various observation substances, and performing one-time targeted training on the two models; simulating the temporal and spatial change state of the current substance based on the model parameter set corresponding to each substance, analyzing the synthesis center based on simulation data, and analyzing the key gene by comparing the gene sequencing results of the synthesis center / comparison area of each substance. According to the invention, the positioning accuracy of the synthesis center and the identification accuracy of key gene information can be improved.

The invention discloses a 5 'UTR library, a recombinant expression vector and a genetically engineered bacterium for improving the yield of folamin in pathogenic bacterium Badei and application of the 5' UTR library and the genetically engineered bacterium, and belongs to the technical field of microbial genetic engineering. The method comprises the following steps: designing an SD region based on a 16S rRNA (ribosomal Ribonucleic Acid) 3'end complementary sequence of a Buddei pathogenic bacterium XBD101 serving as an original strain, regulating and controlling the number of bases between the SD sequence and an initiation codon AUG and optimizing an UTR secondary structure to construct a library containing 14 UTR sequences, and replacing three sequences N4A, N7A and N18A with the strongest translation ability in the library to the UTR region of a folamin synthesis key gene fclC, so as to obtain the folamin peptide. And finally, the capacity upgrading of the folamin chassis strain is realized, and an important support is provided for the industrial production of agricultural antibiotics.

The invention discloses a rape grain weight regulation gene NGAL3 based on whole genome screening and a breeding application thereof, based on the seed size and grain weight phenotypic value of a cabbage type rape germplasm resource, a candidate gene interval is positioned by using GWAS; the method comprises the following steps: selecting large-grain-weight and small-grain-weight extreme phenotypic materials, performing transcriptome sequencing in a critical period of seed development, and screening differential expression genes; and performing cross comparison on the GWAS candidate gene and the differential expression gene to obtain a key gene for jointly regulating and controlling the size and the grain weight of the seed, and performing functional verification on the key gene. The thousand seed weights of the created homozygous three mutant strains L1-sg1-1-4-5 and L2-sg1-4-8-16 are obviously increased by 46% and 29% compared with those of the wild type strains. The invention provides a new target for high-yield rape breeding, and non-transgenic high-grain-weight germplasm can be created through gene editing or beneficial allelic variation of NGAL3 is selected and enriched under the assistance of molecular markers.

The invention relates to a male gamete killer gene S19g-A1 of a rice interspecific hybrid sterile S19 seat and application of the male gamete killer gene S19g-A1. The invention provides a rice hybrid sterility related protein S19g-A1. The amino acid sequence of the rice hybrid sterility related protein S19g-A1 is as shown in SEQ ID NO. 2. According to the invention, a key gene S19g-A1 of a rice interspecific hybrid sterile S19 site is researched and identified, function knockout is carried out on African rice S19g-A1, and pollen fertility of a mutant hybrid F1 obtained by carrying out distant hybridization on an obtained African rice s19g-a1 mutant returns to normal. Based on a gene editing technology, the invention develops a method for rapidly creating a hybrid affinity line capable of overcoming sterility of African rice hybrid, effectively eliminates S19 locus mediated interspecific hybrid reproductive disorder, breaks through the bottleneck of rice interspecific hybrid advantage utilization, provides target gene resources for distant hybrid advantage utilization and crop yield improvement, and has a wide application prospect. Good application prospects are realized.

The invention discloses an application of an NtMADS21 gene in regulation and control of tobacco traits. The nucleotide sequence of the NtMADS21 gene is shown as SEQ ID NO. 1. The invention also discloses an application of the NtMADS21 gene in regulation and control of tobacco traits. Through overexpression of the NtMADS21 gene, it is found that the NtMADS21 gene can synergistically improve the plant type and the tissue culture character of tobacco, specifically, the plant height is reduced, the number of leaves is increased to shape an ideal plant type, the leaf callus induction capacity is remarkably improved to 78.5%, in addition, the NtMADS21 gene has the effect of delaying flowering, and the yield of tobacco is increased. The invention provides a key gene resource integrating various excellent characters for genetic improvement and efficient breeding of tobacco.

The invention provides application of a BnaXPT gene in regulating and controlling the content of oil and protein in rape seeds. According to the invention, the key gene XPT gene for regulating and controlling the balance of the oil content and the protein content of the rape seeds is excavated on the whole genome level through multi-omics association analysis, and by constructing an XPT overexpression strain and an XPT function-deficient rape variety, it is found that the protein content of the rape seeds can be remarkably improved through overexpression of the gene; by mutating the gene, the oil content of rape seeds can be remarkably increased, a brand new target is provided for improving and optimizing the content and balance of the two nutrient substances of the rape, a new way and means are provided for creating the rape with high oil content or high protein content, and the application prospect is wide.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for germplasm identification and breeding of armeniaca mume, and belongs to the technical field of fruit tree molecular marker-assisted breeding. On the basis of re-sequencing and selective clearance analysis of wild plum and armeniaca mume populations, a key gene remarkably related to the armeniaca mume cyanin deletion character is identified, and an SNP variation with a codon terminated in advance exists in a gene coding region; a specific primer is further designed according to the functional SNP variation, a target fragment corresponding to a plum genome is amplified through PCR, and the base type of an SNP site is detected through first-generation sequencing, so that whether the detected plant is the armeniaca mume germplasm or not can be quickly and accurately judged. The SNP molecular marker can be used for early screening of armeniaca mume germplasm, has important application value, and provides an efficient and reliable technical means for molecular marker-assisted breeding.

The invention relates to the technical field of microbial directional cultivation, in particular to a gene editing-based MICP functional bacteria directional cultivation method and system, and the method comprises the following steps: obtaining an original strain library with MICP activity, carrying out strain activation on the original strain library, carrying out high-throughput sequencing on the original strain library, and carrying out gene mining on an initial sequence; the method comprises the following steps: carrying out gene editing on strains in an initial strain library by using a gene editing tool, carrying out shake-flask activation on engineered candidate strains to obtain an activated recombinant bacterium solution, carrying out high-density culture on the activated recombinant bacterium solution by using a pre-constructed fermentation tank, screening out an MICP strain with the optimal function, carrying out continuous passage bacterium function detection on the optimal MICP strain, and carrying out high-density culture on the MICP strain with the optimal function. And based on the MICP functional bacteria, finishing directional cultivation of the MICP functional bacteria based on gene editing. According to the invention, directional cultivation of the MICP functional bacteria is efficiently realized by accurately targeting the key gene with the MICP function, and the MICP functional bacteria are corrected when the problems of function instability and the like exist, so that the more stable MICP functional bacteria are obtained.

The present application provides an application of inhibiting or knocking out tobacco polyphenol oxidase gene NtPPO12 in inhibiting tobacco browning. The present application screens an important gene NtPPO12 related to tobacco browning through transcriptome sequencing and molecular biology experiments, further verifies the promoter activity and gene expression site of NtPPO12, and deeply researches the function of the gene through gene editing and overexpression. The present application proves by molecular biology experiments that the NtPPO12 gene provided by the present application is a key gene related to browning in the mature curing process of tobacco, and by regulating the expression amount of the NtPPO12 gene, the browning degree of tobacco can be controlled, and the tobacco maturity and curing resistance can be improved, which is beneficial to reducing the picking frequency, improving the curing characteristics, and improving the appearance and internal quality of tobacco leaves, so it has a broad market application prospect.

The invention discloses a key gene AaMYB114 closely linked with the color character of an actinidia arguta fruit and application of the key gene AaMYB114. The AaMYB114 gene disclosed by the invention is positioned at 5237694-5239966bp of a chromosome 9 of an actinidia arguta genome, and the full length of the AaMYB114 gene is 2273bp. According to the application, all-red type and all-green type actinidia arguta are used as materials for transcriptome sequencing, and the gene AaMYB114 related to the formation of the peel color of the actinidia arguta is explored. Instantaneous conversion of the actinidia arguta fruit proves that the overexpression of the AaMYB114 increases the anthocyanin content in the actinidia arguta peel. Yeast one-hybridization and dual-luciferase report tests prove that the AaMYB114 not only interacts with a promoter of AaGST, activates the expression of the promoter and regulates anthocyanin transport of peel tissues of actinidia arguta, but also is combined with promoters of anthocyanin synthesis structural genes AaPAL, AaCHI and AaF3H and activates the expression of the promoters to promote anthocyanin synthesis. Therefore, the AaMYB114 gene plays a dual regulation and control role in the formation of peel color and luster, and can be used for cultivating red-peel actinidia arguta.

The present application belongs to the technical field of genetic engineering, and particularly relates to a methyltransferase gene CmCMT2 and CmDRM2 application in regulating chrysanthemum flowering. The present application constructs an RNAi silencing expression vector by isolating CmCMT2 and CmDRM2 genes in a chrysanthemum variety "Jinma", and obtains CmCMT2 -RNAi and CmDRM2 -RNAi transgenic "Jinma" plants by using a genetic transformation method, then performs phenotype observation and statistics on the transgenic plants, and explores CmCMT2 and CmDRM2 the mechanism of the genes affecting early flowering of chrysanthemum by using an RNA-seq method. The CmCMT2 and CmDRM2 genes are cloned from the chrysanthemum variety "Jinma", and spatiotemporal expression analysis shows that CmCMT2 and CmDRM2 the genes are highly expressed during the process of "Jinma" from bud development to color change; and CmCMT2 and CmDRM2 the expression level in leaves gradually decreases during the growth and development of "Jinma". The research results show that CmCMT2 and CmDRM2 the content of GA1 is changed by negatively regulating the expression of CmG20ox2a and CmG20ox2b , so that the flowering period of chrysanthemum is advanced by 15 and 8 days, respectively.

The invention discloses a BcC3H33 gene of flowering cabbage and an application of a protein encoded by the BcC3H33 gene in resisting cadmium stress. The invention provides a new application of the BcC3H33 gene of the flowering cabbage and the encoded protein thereof in regulation and control of growth and development of the flowering cabbage and cadmium stress response, and research shows that the BcC3H33 gene has very strong response to cadmium stress in roots and leaves of the flowering cabbage and can regulate and control the growth and development of the flowering cabbage and promote the growth of the flowering cabbage; after the BcC3H33 gene is over-expressed in the flowering cabbage, the biological accumulation amount of the flowering cabbage is increased, the morphological index is improved, the photosynthetic ability is enhanced, the stress resistance is enhanced, the influence of cadmium stress on the growth and development of the flowering cabbage can be relieved, and the growth of the flowering cabbage can also be promoted under the cadmium stress. Therefore, the BcC3H33 gene can improve the cadmium stress tolerance of the Chinese flowering cabbage, improve the crop adaptability, provide a basis and a method for stress-resistant breeding of the Chinese flowering cabbage, provide key gene resources for the Chinese flowering cabbage and other crops, and meet different cultivation environment requirements.

The invention belongs to the technical field of animal genetic breeding, and provides an application of an SNP molecular marker related to a pig backfat thickness character. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 7, wherein the polymorphism of the eleventh basic group is C / T. The key gene SLC26A3 related to the backfat thickness of the large white pig is identified through whole genome deep sequencing, the genotype of the backfat thickness character of the large white pig is further detected through an SLC26A3 gene molecular marker, and the backfat thickness of the CC genotype large white pig is obviously higher than that of a CT / TT genotype pig. Therefore, the breeding of the CT / TT genotype individuals is beneficial to meeting the continuous demand of large-scale production on high-efficiency utilization of feed energy. The detection method provided by the invention is simple and easy to operate, can efficiently and quickly identify the back fat thickness character of the large white pig, improves the uniformity of the back fat thickness of the pig, and provides a scientific basis for early breeding of the pig.