105 results about "Key genes" patented technology

This invention belongs to the field of bioinformatics and relates to a single-cell trajectory inference method based on adaptive feature selection. First, an initial gene expression matrix is ​​obtained through data preprocessing and screening for highly variable genes. Second, a two-dimensional evaluation strategy is employed to calculate the scores of highly variable genes with gene expression variability and the trajectory importance score related to differentiation trajectories. Then, a dynamic weight fusion mechanism is introduced, adaptively adjusting the fusion weights of the two scores based on performance feedback, and highlighting key genes through nonlinear enhancement. Next, an intelligent inflection point detection algorithm adaptively determines the optimal number of features. Finally, trajectory inference is performed based on a variational autoencoder model reconstructed from feature subsets, and a performance-driven feature selection closed loop is formed through multiple rounds of iterative optimization. This invention achieves high-precision, adaptive single-cell trajectory inference, solving the technical problems of single feature selection and fixed weights in traditional methods.

This invention discloses a method for constructing a prognostic model for acute myeloid leukemia (AML) based on ferroptosis-related genes. The method involves acquiring gene expression data from AML patients and healthy samples to screen for differentially expressed ferroptosis-related genes (DEGs) associated with AML. Using univariate Cox proportional hazards regression analysis, LASSO regression analysis, and multivariate Cox proportional hazards regression analysis, key genes ACSF2, SLC7A11, DNAJB6, and SOCS1 are selected from these DEGs. A prognostic risk model is constructed based on the expression levels of these key genes and their corresponding multivariate Cox regression coefficients. The risk score formula is: Risk Score = 0.534 × ACSF2 expression value - 0.453 × DNAJB6 expression value + 0.194 × SLC7A11 expression value + 0.308 × SOCS1 expression value. The prognostic model constructed in this invention has high predictive accuracy and reliability, effectively stratifying the risk and assessing the prognosis of AML patients, providing an important reference for the clinical treatment of AML.

This invention discloses PtoNAC028 The gene is used to regulate the ratio of pectin monomers in poplar trees. The coding region of this gene has the nucleotide sequence shown in SEQ ID NO:1. This is achieved through overexpression or silencing of the gene in poplar trees. PtoNAC028 Genes capable of increasing or decreasing the proportion of lignin monomers in poplar provide key genes for the genetic improvement of poplar processing traits. This invention also discloses a method for cultivating poplars with a high lignin monomer ratio, through overexpression of [gene name missing] in poplar trees. PtoNAC028 The gene can significantly increase the proportion of lignin monomers in poplar trees, achieving targeted regulation of monomeric lignin metabolism, which helps to rapidly cultivate new poplar varieties with excellent wood properties and fast growth.

The application discloses application of an exogenous hormone methyl jasmonate in improving quality of peony seed oil and a method thereof, a hormone response element in a key gene promoter of oil synthesis is analyzed, an exogenous hormone with regulation potential is screened, and the optimal exogenous hormone is MeJA; when spraying is performed twice at a spraying concentration of 100 μmol / L, the content of peony seed oil is significantly improved, and alpha-linolenic acid is also significantly improved, so that dual improvement of peony seed oil yield and quality is finally realized.

This invention provides SSR molecular markers associated with the yield trait of Corydalis yanhusuo, belonging to the field of molecular genetics. This invention utilizes 24 pairs of SSR markers to perform regression analysis on the genotype and yield trait of individual Corydalis yanhusuo individuals. Using the GLM program, association loci for the yield trait of Corydalis yanhusuo were detected. The SSR molecular markers CYC2-4 and CYC8-10 were found to be highly significantly associated, explaining 16.72% and 14.06% of the phenotypic variation, respectively. The primers provided by this invention for amplifying the aforementioned SSR molecular markers can be used to screen for high-yielding Corydalis yanhusuo germplasm, locate key genes controlling yield traits, and for marker-assisted breeding, which is of great significance for cultivating high-yielding Corydalis yanhusuo varieties.

The application belongs to the field of plant genetic engineering, and discloses a key gene for positively regulating salt stress and oxidation stress tolerance of rice OsHPCA1 . The gene (accession number: Os05g40770) encodes a receptor protein specifically sensing hydrogen peroxide, which is classified into the leucine repeat receptor-like kinase (LRR-RLKs) family, which is the largest subfamily in the receptor-like kinase (RLKs) family. OsHPCA1 In response to salt and oxidation stress signals, the rice salt and oxidation stress tolerance is positively regulated. OsHPCA1 The overexpression rice strain shows strong salt tolerance; and OsHPCA1 the knockout strain oshpca1 is more sensitive to salt stress. OsHPCA1 The leucine repeat receptor protein kinase OsHPCA1 encoded by the gene can positively regulate the activity of catalase C (CatC) in the rice body, enhance the H2O2 clearance efficiency under salt stress, and thus improve the salt tolerance of the rice. When OsHPCA1 overexpressed, the activity of CatC in the overexpression strain OsHPCA1 is significantly increased, the H2O2 clearance capacity is enhanced, and the salt tolerance and oxidation stress tolerance of the rice are improved.

This invention provides a tandem gene, expression cassette, expression vector, and rice germplasm construction method for synthesizing ginsenoside Ro, belonging to the field of genetic engineering technology. This invention provides a tandem gene for synthesizing ginsenoside Ro, which involves tandemly connecting four key genes from *Saccharomyces cerevisiae*, one gene from *Licoricea javanica*, and five genes from ginseng, and constructing gene expression units for each, thereby forming a tandem expression cassette. This invention connects the tandem expression cassette with a plant expression vector to construct a recombinant expression vector, transforms the recombinant expression vector into *Agrobacterium*, and successfully transforms the recombinant expression vector into the rice genome using an *Agrobacterium*-mediated genetic transformation method. The rice can express the recombinant expression vector correctly and efficiently in the grains, laying a solid technical foundation for the large-scale synthesis of ginsenoside Ro.

The application discloses a chrysanthemum constitutive high-expression promoter and application thereof in gene editing. The nucleotide sequence of the promoter CmUbi is shown as SEQ ID NO. 4. Through double luciferase reporter experiments and stable genetic transformation experiments, it is proved that the expression activity of the CmUbi promoter in chrysanthemum protoplast and callus is significantly higher than that of commonly used CaMV 35S, AtRPS5a and corn ZmUbi promoters. A CRISPR / Cas9 gene editing vector is constructed by using the promoter, and a key gene of branch of hexaploid chrysanthemum is edited BRC1b , and a plurality of allele simultaneous mutation complete editing strains are successfully obtained, and the number of branches of the mutants is significantly increased. The endogenous high-activity promoter of the chrysanthemum provided by the application effectively solves the problems of low expression activity of an existing heterologous promoter in the chrysanthemum and poor gene editing efficiency of a polyploid, and provides an efficient and adaptive expression regulation element for chrysanthemum molecular breeding and functional genomics research.

This invention discloses the application of the OsSR1IP1 gene in regulating tillering angle in rice. It belongs to the field of plant genetic engineering technology. This invention uses genetic engineering technology to obtain transgenic rice that stably overexpresses OsSR1IP1. By comparing the growth of wild-type and OsSR1IP1-overexpressing transgenic rice, it was found that the tillering angle of OsSR1IP1-overexpressing transgenic rice plants was significantly increased. Therefore, this invention successfully identified a key gene regulating rice plant architecture, which has theoretical significance and application value for enhancing the lodging resistance and increasing yield of rice varieties, and is of great importance to agricultural development and national food security.

This application discloses a recombinant bacterial strain that highly expresses nonspecific peroxygenase, its construction method, and its applications, relating to the field of microbial engineering technology. The strain of this invention uses *Escherichia coli* BL21(DE3) as the host. First, the key gene *hemA* for heme synthesis is integrated into the genome using the CRISPR-transposon system. Then, a chassis engineered strain A2 with 2 copies of optimal integration is screened and co-transformed with the rDcaUPO-A161C expression plasmid and the molecular chaperone plasmid pG-KJE8. The intracellular heme level of this strain is increased by 2.84 times compared to the original strain, and the total enzyme activity of rDcaUPO-A161C is cumulatively increased by 8.9 times and the specific enzyme activity is increased by 6.21 times compared to the wild type. This fundamentally solves the problems of insufficient cofactors, low folding efficiency, and poor activity in heterologous expression of nonspecific peroxygenases, and can be used for the efficient production of nonspecific peroxygenases.

This invention relates to the field of pest control technology, and discloses a double-stranded RNA and its application in the control of citrus aphids. The double-stranded RNA comprises a double-stranded RNA consisting of the nucleotide sequence shown in SEQ ID NO:2 and its reverse complementary nucleotide sequence; the nucleic acid molecule encoding the double-stranded RNA is shown in SEQ ID NO:1 or SEQ ID NO:3. The double-stranded RNA provided by this invention can simultaneously target the chitin synthase gene, cytochrome P450 monooxygenase gene, and epidermal protein gene of the brown citrus aphid, and simultaneously silence the expression of these three key genes through an RNA interference mechanism, leading to inhibited growth and development or death of the brown citrus aphid. Therefore, the double-stranded RNA provided by this invention can efficiently and specifically control the brown citrus aphid; it can reduce the use of chemical pesticides, is green and environmentally friendly, and safe and harmless.

This invention belongs to the field of plant genetic engineering, specifically disclosing a method for creating bifunctional haploid inducible lines of Brassica napus based on the BnaKNL2 gene and its application. Research revealed that the BnaKNL2 gene is a key gene affecting the fertility of Brassica napus. By knocking out the KNL2 gene in Brassica napus, bifunctional haploid inducible lines with maternal or paternal haploid induction capabilities can be created. The haploid induction rate after hybridization with different Brassica napus varieties can reach 0.45%–2.7%. Furthermore, by combining this with a cytoplasmic male sterility system and through hybridization and backcrossing, the CMS-HI line, carrying both sterile cytoplasm and haploid induction capabilities, can be rapidly created. This provides a new approach for haploid breeding of Brassica napus and the improvement and creation of CMS lines, with broad application prospects.

The application discloses a method for predicting different metabolic states of cells in cancer and metabolic characteristic subtypes of the cells based on a metabolite-protein interaction network, and the method comprises the following steps: (1) identifying the classification and metabolic state of the cells: preprocessing data, filtering low-quality cells; performing twice principal component analysis according to key genes; performing twice dimension reduction clustering on the cells respectively; (2) predicting metabolic characteristic subtypes of a queue according to the proportion of different metabolic states of cells in the sample. The method can identify the metabolic state of the cells and metabolic subtypes, and provides a new perspective for cancer research.

The application relates to the technical field of biological information, and discloses a plant stress relief key gene tracing method and system based on transcriptome data. The method comprises the following steps: performing high-throughput transcriptome sequencing on plant leaves of four treatment groups, obtaining clean transcriptome data through quality filtering; obtaining a whole genome expression matrix through transcript assembly and redundancy removal processing, performing intersection screening on stress induction up-regulated genes and relief agent silencing down-regulated genes, and obtaining a stress induction-relief agent silencing candidate gene set; constructing a stress induction co-expression topology network by taking the candidate gene set as a silencing annotation basis, calculating a weighted neighbor silencing rate to obtain a topology feature matrix; training a selective silencing probability prediction model through a logistic regression model by taking the topology feature matrix and the candidate gene set, and performing channel enrichment calculation by taking the silencing probability as a weight to screen and obtain a core tracing gene set. The application improves the biological credibility of key gene tracing results and the cross-scene adaptability of the method.

The application discloses genetically engineered bacteria for synthesizing L-tryptophan and application thereof. The application realizes the following through two key gene regulation strategies (serA down-regulation and serB up-regulation): the accumulation amount of glutamic acid by-products is significantly reduced from 8-20 g / L to below 1.14 g / L, and the reduction ranges are 87.74% and 90.3% respectively; the sugar acid conversion rate (glucose / L-tryptophan) of L-tryptophan is increased from 19.2% to 20.9% and 21.3% respectively; without additional addition of expensive cofactors or inducers, the cost-effectiveness is significant.

This invention provides SSR molecular markers associated with the proliferation trait of Corydalis rhizomes, belonging to the field of molecular genetics. Trait correlation analysis shows that Corydalis rhizomes proliferation is a key factor affecting its yield. This invention utilizes 24 pairs of SSR markers to perform regression analysis on the individual genotype and rhizome proliferation trait of Corydalis rhizomes. The association loci for Corydalis rhizomes proliferation were detected using the GLM program, explaining 18.73% of phenotypic variation, with the SSR molecular marker CYC2-8 showing a highly significant correlation. The primers provided by this invention for amplifying the aforementioned SSR molecular markers can be used to screen for superior Corydalis rhizomes with high rhizome proliferation, locate key genes controlling the rhizome proliferation trait, and for marker-assisted breeding, which is of great significance for cultivating high-yielding Corydalis rhizomes varieties.

This invention belongs to the field of plant breeding and genetic engineering technology, specifically involving AtGalOxs Genes and their application in regulating plant organ size and development. Specifically, this invention screened a gene that regulates plant organ size and development. AtGalOxs It was identified as a key gene regulating the size and development of organs in Arabidopsis thaliana plants, and this was confirmed through cytological observation and transcriptome analysis. AtGalOxs Genes function by regulating cell proliferation and auxin signaling pathways, providing new theoretical basis for elucidating the molecular network regulating plant organ size; AtGalOxs The gene can be directly applied to the regulation of Arabidopsis plant architecture, and its homologous genes are conserved in crops such as Brassicaceae. This provides key gene resources for improving crop plant architecture and increasing yield, which is of great significance for promoting the development of plant molecular breeding technology and improving agricultural production efficiency.

This invention belongs to the field of microbial metabolic engineering and industrial biotechnology, and relates to an engineered *Pichia pastoris* strain utilizing xylose as the sole carbon source, its construction method, and its applications. Specifically, it involves reconstructing the xylose assimilation pathway within the host strain by introducing genes for the XI-XK pathway, the non-oxidative phase of the PPP pathway, and the PK-PTA pathway into the *Pichia pastoris* host strain, thus obtaining the engineered strain; or, culturing the above-obtained engineered strain in a culture system utilizing xylose as the sole carbon source to obtain an engineered *Pichia pastoris* strain with xylose as the sole carbon source; or, optimizing key gene targets and / or metabolic pathways of the above-obtained engineered strain or the engineered *Pichia pastoris* strain with the sole carbon source to obtain an engineered strain. This invention provides a method for obtaining a *Pichia pastoris* strain capable of using xylose as the sole carbon source, which enhances the xylose assimilation pathway through metabolic reconstruction, thereby improving xylose utilization.

This invention provides a type of yeast called *Saccharomyces clavatum*, characterized in that the *Saccharomyces clavatum* is: *Saccharomyces clavatum* (… Saccharomycopsis fibuligera AMCC 32248, preservation number CCTCC NO: M 20251908. Enzyme activity phenotypic screening, carbon source utilization analysis, and quantitative enzyme activity detection showed that this strain possesses highly efficient β-glucosidase activity; further analysis of key genes related to β-glucosidase... BGL1 Specific functional sequences were extracted and identified. Enzyme production conditions were optimized using response surface methodology, resulting in a significant increase in enzyme activity compared to before optimization. In a simulated fermentation system, this strain significantly promoted the production of specific acids, esters, aldehydes, alcohols, and other volatile flavor compounds. Its enzyme production characteristics and flavor regulation capabilities provide a new technical solution for addressing issues such as low hydrolysis efficiency of glycosides and insufficient flavor release in fermentation systems, showing potential applications in the food, brewing, feed, and pharmaceutical industries.

This invention belongs to the field of molecular breeding technology and provides a method based on... C12orf4 Molecular breeding methods and applications for genetic variations of the eQTL gene in eggshell strength and anti-aging resistance. This invention utilizes molecular marker combinations... C12orf4 Gene expression levels have a regulatory role; the molecular marker combination is located at Chr1:72995066, Chr1:73250348, and Chr1:73614612 loci in the chicken genome, respectively. This invention, through multidimensional association analysis integrating genomic, transcriptomic, and phenotypic data, identified and constructed a key gene that can prospectively and systematically reflect the process of tissue functional decline in laying hens. C12orf4 The molecular marker combinations provided by this invention offer a new technical means for the accurate assessment of aging-related traits in laying hens and for molecular breeding, and have significant application value and industrial significance.

This invention belongs to the field of plant metabolic engineering and biosynthesis technology, and relates to a recombinant expression vector, engineered bacteria, and their application and method in constructing cotton plants capable of synthesizing artemisinin. This invention integrates five key genes from the artemisinin metabolic pathway—ADS and CYP71AV1 from *Artemisia annua*, and ADH1, DBR2, and ALDH1 from *Bryum arvense*—into a multi-gene overexpression vector for genetic transformation in cotton. Optimal expression strategies for cotton plants capable of synthesizing artemisinin were screened using quantitative real-time radiometric analysis (RCR) and artemisinin content determination. Compared with wild-type plants lacking artemisinin, cotton leaves overexpressing the ADS, CYP71AV1, ADH1, DBR2, and ALDH1 genes showed an artemisinin content as high as 2.34 μg / g DW.

The application belongs to the technical field of corn genetic breeding, and specifically discloses a corn kernel development gene ZmCHR24 and application thereof. ZmCHR24 The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The application obtains a mutant material with loss of gene function through EMS mutagenesis and Mu transposon insertion technology. ZmCHR24 The phenotype analysis result shows that, compared with the wild type, ZmCHR24 the kernel of the mutant with loss of gene function presents severe development defects, and specifically, the kernel is only pericarp with no embryo and endosperm. ZmCHR24 The above result shows that the gene is a key gene for regulating corn kernel development. The application provides an important theoretical basis and gene resource for genetic improvement and molecular design breeding of the yield trait of corn kernels.

This invention discloses a rice grain-per-panicle number gene, OsTT2, and its applications, belonging to the fields of plant genetic engineering and molecular breeding technology. Through CRISPR / Cas9 gene editing experiments, this invention demonstrates that knocking out the OsTT2 gene significantly reduces the grain-per-panicle number in rice. It also elucidates the molecular mechanism by which OsTT2 influences grain-per-panicle number by directly binding to and activating the co-regulatory factor OsMED15a promoter, thereby regulating downstream grain shape development genes. Furthermore, this invention identifies for the first time a 331 bp fragment duplication variant (SD2) in the OsTT2 gene promoter region associated with the high grain-per-panicle number trait, and based on this, develops molecular markers for selection assistance and corresponding rice breeding methods. This invention provides new key gene resources and precision breeding tools for high-yield rice breeding.

The application belongs to the technical field of biology, and discloses a transcription factor SlNAC078 for regulating flavonoid synthesis of tomatoes and application. The CDS sequence of the transcription factor SlNAC078 is shown as SEQ ID NO. 1, the transcription factor SlNAC078 can positively regulate flavonoid synthesis of tomatoes, overexpression of the gene can activate the promoters of SlCHS1, SlCHS2 and SlFLS genes, and significantly improve the flavonoid content in fruits; and the transcription factor SlNAC078 can also improve the root dry weight and single fruit weight of tomato plants, and realize the synergistic improvement of nutritional quality and yield. The transcription factor SlNAC078 for regulating flavonoid synthesis of tomatoes and application directly targets the regulation mechanism of key genes for flavonoid synthesis, the cultivation method is simple, the obtained transgenic line is genetically stable, and the transcription factor SlNAC078 provides a key gene target and technical scheme for tomato breeding, and has an important application prospect in the industrialized production of high-nutrition and high-yield tomato products.

This invention discloses TaALDH7 Application of genes in regulating crop resistance to stripe rust and breeding disease-resistant varieties. This invention screened key genes regulating wheat resistance to stripe rust through combined transcriptomic and metabolomic analysis. TaALDH7 Through transient overexpression and gene silencing technology, TaALDH7 The function of the gene in wheat resistance to stripe rust was analyzed; the experimental results showed that, compared with the control group, the gene was overexpressed in wheat. TaALDH7 The disease index and fungal biomass of the gene were significantly reduced, and the colony area and hyphal length of stripe rust fungus were significantly decreased; conversely, in silent wheat... TaALDH7 The gene significantly reduces the resistance response of wheat to stripe rust, indicating that... TaALDH7 Genes act as positive regulators in the wheat's resistance to stripe rust. This invention shows promise for applications in breeding stripe rust-resistant wheat varieties and improving wheat's resistance to stripe rust fungi.

This invention relates to a recombinant construct comprising: one or more TMV Ω enhancers; and a group of fluorescent factors configured to enable an organism or its tissues or cells to emit bioluminescence; the recombinant construct is configured to increase the biosynthetic content of caffeic acid in an organism or its tissues or cells, or to increase the bioluminescence intensity of an organism or its tissues or cells. This application, by introducing TMV Ω enhancers, achieves precise regulation of these two key genes, significantly improving bioluminescence intensity and solving the problems of poor targeting and limited synergistic effects of enhancer applications in existing technologies.

The application belongs to the technical field of plant genetic engineering, and particularly relates to a CmMADS1 gene and application thereof in regulating fruit ripening. The CmMADS1 gene is cloned from a melon, and the nucleotide sequence of the CmMADS1 gene is shown as SEQ ID NO:1. By overexpressing the CmMADS1 gene in tomatoes, it is confirmed that the gene can significantly promote fruit early ripening, and the fruit ripening time is advanced by 3-5 days, and the growth cycle of the tomatoes is significantly shortened. The application provides a new key gene resource for melon fruit ripening regulation research, and opens up a new path for molecular breeding of melon early-ripening varieties.

This invention relates to the fields of gene editing and animal genetic breeding technology, and discloses a precise editing method for key genes regulating lipid metabolism in cattle and sheep. The method includes: constructing a comprehensive lipid metabolism network map integrating transcriptomic, proteomic, and metabolomic data; using a graph neural network to identify key node genes with high flow weights and their negative feedback pathways; simulating cascade reactions after gene perturbation to assess compensatory effects; screening for multi-gene combinations that do not induce significant compensation and can synergistically regulate fat deposition and muscle growth; designing expression vectors containing multiple guide RNA sequences to achieve simultaneous and precise editing; and obtaining edited individuals and validating the phenotype through embryonic or somatic cell nuclear transfer. This invention, through system-level multi-target synergistic editing, avoids metabolic imbalances, improves editing effectiveness, and promotes muscle development while reducing fat deposition, providing a safe and efficient technical path for precision breeding of cattle and sheep.

The application discloses a neural network calculation method and device for gene expression regulation analysis, and relates to the technical field of bioinformatics.The method comprises the following steps: obtaining first feature data and second feature data; constructing input features containing multiple regulation levels; inputting the input features of the multiple regulation levels and the second feature data into a target neural network model, and outputting a prediction result of a gene expression state.The neural network calculation method provided by the application can deeply integrate chromatin accessibility and three-dimensional spatial interaction data through a dynamic routing module, can solve the "black box" problem that a traditional deep learning model is inaccurate and difficult to explain, and can accurately identify key gene regulation pathways in a manner of explicitly simulating real biological regulation mechanisms.