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487 results about "Key genes" patented technology

KEY WORDS GENETICS GENOTYPE The genetic makeup of an organism It describes the organism in terms of the alleles it contains, usually in the context of a particular characteristic An organism with two identical alleles for a gene is homozygous. An organism with two different alleles for the same gene are heterozygotes.

Construction of corynebacterium glutamicum engineering bacteria for high-yielding production of L-valine and method for fermentation production of L-valine

The invention discloses construction of a strain of corynebacterium glutamicum engineering bacteria for high-yielding production of L-valine and a method for fermentation production of L-valine, and belongs to the technical field of food biological engineering. Corynebacterium glutamicum ATCC13869 is used as an original bacterial strain, knockout combination of genes of aceE, alaT and ilvA is carried out, and an ATCC13869[delta]aceE[delta]alaT[delta]ilvA mutant bacterial strain is obtained and named as WCC003; expression combination of genes of lrp1, brnFE and ilvBNC1 is carried out on the WCC003, and expression-combined engineering bacteria WCC003/pJYW-4-(ilvBNC1)-lrp1-brnFE are obtained and preserved in China center for type culture collection with the preservation number of CCTCC NO:M2014149; the expression-combined engineering bacteria are used for fermentation production of L-valine. The corynebacterium glutamicum engineering bacteria based on the expression regulating protein Lrp, the transfer protein BrnFE and the L-valine synthetic route key gene ilvBNC1 and used for high-yielding production of L-valine are provided, advantageous mutation Arg39Trp of Lrp in corynebacterium glutamicum is clear and definite for the first time, a fact that Lrp and BrnFE are used for fermentation production of L-valine is also reported for the first time, and the study on the expression combination of the genes of lrp1, brnFE and ilvBNC1 is also created for the first time.
Owner:JIANGNAN UNIV

Method for screening cattle high altitude hypoxia adaptation molecular markers and application thereof

ActiveCN109994153AEfficient and accurate screeningPrecise screeningMicrobiological testing/measurementProteomicsHigh altitude hypoxiaGenome evolution
The invention provides a method for screening cattle high altitude hypoxia adaptation specific molecular marker, and furthermore a cattle kind or individual which is suitable for high altitude hypoxiasurvival is screened through the specific marker. The method aims at a high altitude hypoxia adaptation heredity characteristic of a kettle kind, and selects local cattle kinds which are distributedin high altitude regions at aspects of genome evolution, selecting and adapting. Multiple genome selecting signals, a full-genome association analysis method and a strategy are combined. Key genes andmolecular makers which are suitable for high altitude hypoxia are efficiently and accurately screened. Reasonable method designed is realized. The detecting method according to the key gene and marker designing has advantages of high accuracy, and convenient application operation. According to the method of the invention, through analyzing different altitude cattle kinds, a gene ACSS2 which is related with high altitude hypoxia adaptation and a haplotype thereof are found; and furthermore a specific SNP which bears a strongest selecting signal is positioned; and the method realizes an important meaning and a high practicability value for cattle molecule breeding operation.
Owner:DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI +1

Molecular combination probe for diagnosing and screening chromosome microdeletion syndrome

The invention belongs to the technical field of biology, and particularly relates to a molecular combination probe for diagnosing and screening chromosome microdeletion syndrome. The molecular combination probe is used for selecting the key gene of the Williams syndrome, the 22q11 microdeletion syndrome, the Prader-Willi syndrome, the Angelman syndrome, the 15q13.3 microdeletion syndrome and the Rett syndrome, or the gene within a critical area, or the gens arranged at two ends within a duplication/deletion fragment, selecting the sequence which meets a corresponding condition as a probe sequence according to the sequence of the gene, and adding a general primer sequence and adding a phosphorylation mark to the 5'end of a probe left-half sequence and the 3'end of a right-half probe to prepare the combination probe for the multiple continuous probe amplification technology. According to the combination probe provided by the invention, the defects of the fluorescent quantitative PCR (polymerase chain reaction) can be overcome, a plurality of sequences can be analyzed for once, and the molecular combination probe is higher in resolution ratio, sensitivity and repeatability. The probe can be used for the clinical molecular diagnosing and screening of the six-chromosome microdeletion syndrome.
Owner:FUDAN UNIV

Key genes, microRNAs and other non-coding RNAs or combination thereof used for identifying or regulating cell pluripotency

The invention relates to key genes, microRNAs and other non-coding RNAs, or a combination thereof used for identifying or regulating cell pluripotency. The invention is characterized in that the key genes, microRNAs, other non-coding RNAs or a combination is highly expressed in the stem cell with complete pluripotency and the expression is obviously repressed or silent in the stem cell without complete pluripotency. The genes, microRNAs and other non-coding RNAs are the genes, microRNAs and other non-coding RNAs positioned in the chromosome imprinting region named as Dlk1-Dio3 on the long arm of mouse chromosome 12 and the genes, microRNAs and other non-coding RNAs in the genome collinearity regions of other mammals, which have 70%-100% of homology. The invention also relates to applications of the genes, microRNAs and other non-coding RNAs, or the combination thereof used for identifying the pluripotency of the stem cell and regulating cell pluripotency, applications in stem cell typing, applications for regulating the cell pluripotency and the pluripotency state and level of the cell, applications in disease treatment, and applications in the drug target development of tumor treatment or the development of antitumor drugs.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI +1

Key gene for regulating and controlling chlorophyll degradation in the senescence process of plant and application thereof

InactiveCN101831450AExtend the life of the priceExtended postharvest green periodFermentationPlant genotype modificationBiotechnologyNutrition
The invention relates to a new key gene participating in the regulation and the control of chlorophyll degradation and application thereof, belonging to the technical field of plant gene engineering. Stay-green traits can prolong the commodity price service life of green leafy vegetables and the postharvest green period of fodder crops so as to increase the content of main nutrition constituents, i.e. chlorophylls and proteins; and the stay-green traits can also outstandingly improve the green period and the landscape effect of lawn plants. The invention provides the key gene AtCRN1 for regulating and controlling the chlorophyll degradation and metabolism of plants. An amino acid coding sequence of the key gene AtCRN1 is characterized by being SEQ ID No: 2. The invention also provides a method for establishing a stay-green plant strain, comprising the following steps of: inducing the gene AtCRN1 of a target plant to generate mutation through chemical or physical factors; and destroying or reducing the expression of the gene AtCRN1. Besides, detecting whether the gene AtCRN1 with overall length is contained in a plant genome or not can also be used as a molecular auxiliary breeding method for screening / identifying the stay-green plant strain.
Owner:FUDAN UNIV

Genetically engineered bacterium for producing N-acetylneuraminic acid as well as construction and application of genetically engineered bacterium

The invention belongs to the technical field of genetic engineering, and particularly relates to a genetically engineered bacterium for producing N-acetylneuraminic acid through xylose induction. Escherichia coli is used as a starting strain, an N-acetylglucosamine synthesis pathway is integrated on a genome, an N-acetylglucosamine 2-epimerase gene bAGE and an N-acetylneuraminic acid synthetase gene neuB from collar algae are introduced, an N-acetylneuraminic acid synthesis pathway is constructed, and a key gene nano ATEK of a catabolism pathway of the N-acetylneuraminic acid is knocked out. Meanwhile, metabolic pathways of precursor substances required by synthesis of the N-acetylneuraminic acid are subjected to multi-copy reinforcement, part of bypass metabolic pathways are knocked out, key enzyme genes for producing GlcNAc and Neu5Ac are optimized according to different copy numbers, the optimal proportion of the key enzyme genes is finally determined, and the high-yield strain of the N-acetylneuraminic acid is obtained. The highest yield of the N-acetylneuraminic acid can reach 28g / L, the highest production intensity can reach 0.67 g / (L*h) which is the highest value reported at present, and the N-acetylneuraminic acid has important industrial application value.
Owner:TIANJIN UNIV OF SCI & TECH

Genetically engineered bacterium, and applications thereof in preparation of L-phosphinothricin

The invention discloses a genetically engineered bacterium, and applications thereof in preparation of L-phosphinothricin. The genetically engineered bacterium comprises Escherichia coli and recombinant plasmids transferred into Escherichia coli; the recombinant plasmids contain transaminase gene and pyridoxal phosphate synthetic route enzyme gene; the nucleotide sequence of the transaminase geneis represented by SEQ ID NO.1; the nucleotide sequence of the pyridoxal phosphate synthetic route enzyme gene is represented by SEQ ID NO.2-5. According to the applications, genetic engineering technology is adopted to express ribose-5-phosphate pathway synthesis key gene PdxST in Escherichia coli, or enhance Escherichia coli self PLP synthesis pathway key gene epd, pdxJ, and dxs expression; preparation of the coenzyme reinforced transaminase genetically engineered bacterium through construction is capable of increasing intracellular coenzyme PLP content obviously, avoiding adding of externally-sourced PLP in production of L-phosphinothricin using the genetically engineered bacterium, increasing transaminase enzyme activity and stability obviously, prolonging transaminase half life, reducing production cost, and increasing L-phosphinothricin production efficiency.
Owner:ZHEJIANG UNIV
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