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Culturing method of auxilliary bud less tobacco after topping

A breeding method and tobacco technology, applied in the field of bioengineering, can solve the controversial issues of resistance marker gene ecological environment and food safety

Inactive Publication Date: 2006-08-30
贾朝钧
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the commercialization of genetically modified plants, people worry that herbicide resistance genes in genetically modified crops may be passed to weeds through natural hybridization, and antibiotic resistance genes in genetically modified foods may cause human resistance to these diseases by transfecting intestinal bacteria. Antibiotic resistance, although there is no conclusive and credible scientific evidence, the potential ecological environment and food safety of the resistance marker gene (resistant marker gene) have been controversial, and it is undoubtedly a solution to find a safety marker gene for genetic transformation of plants. The best approach to this problem

Method used

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  • Culturing method of auxilliary bud less tobacco after topping
  • Culturing method of auxilliary bud less tobacco after topping
  • Culturing method of auxilliary bud less tobacco after topping

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Embodiment Construction

[0297] The cloning process of tobacco wound-inducible peroxidase gene (tpoxN1) promoter in the inventive method is as follows:

[0298] 1. Materials and methods

[0299] 1.1 Plant material: Tobacco (Nicotiana tabacum cv.K326) seeds are preserved by the State Key Laboratory of Tropical Crops Biotechnology, Chinese Academy of Tropical Agricultural Sciences; the young leaves are collected for genomic DNA extraction;

[0300] 1.2 Reagent: Universal Genomewalker TM Kit and Advantage Genomic Polymerase Mix were purchased from CLONTECH; IPTG, X-Gal, dNTPs, and pGEM-T easy Vector were purchased from Promega; other biochemical and conventional reagents were ultra-pure and analytically pure;

[0301] 1.3 Method

[0302] 1.3.1 Extraction of tobacco genome DNA: according to the method of Fu Rongzhao (Fu Rongzhao, Sun Yongru, Jia Shirong. Handbook of Plant Genetic Transformation Technology. Beijing: China Science and Technology Press, 1994, 140-142);

[0303] 1.3.2 Construction of Geno...

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Abstract

The present invention discloses a tobacco cultivation method. Said method is characterized by that it adopts a molecular biological technique to identify key gene capable of promoting axillary bud germination and growth after the tip is pruned, then uses nocuity inducible promoter to drive antisense RNA and make it promptly express after the tip is pruned so as to inhibit the expression of said key gene capable of promoting axillary bud germination and growth and make the axillary bud can not be germinated or grown.

Description

Technical field: [0001] The invention relates to a method for cultivating tobacco without axillary buds after topping, and belongs to the technical field of bioengineering. Background technique: [0002] Tobacco is used as a leaf crop, and letting it bloom and bear fruit will consume a lot of nutrients and reduce the yield and quality of tobacco leaves. In the cultivation of high-quality flue-cured tobacco, "topping" is an important measure to improve the quality of tobacco leaves. The top buds or inflorescences must be removed at an appropriate time to prevent unnecessary consumption of nutrients in the tobacco plant and promote the concentrated supply of nutrients for leaf growth. , increasing leaf area and single leaf weight. [0003] However, after topping, the axillary buds will grow in clusters, which will consume nutrients and fail to achieve the purpose of improving the quality of tobacco leaves. In actual production, the axillary buds are usually suppressed by man...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09C12N15/29C12N15/82A01H4/00A01H1/02
Inventor 贾朝钧陈守才
Owner 贾朝钧
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