3772 results about "Cell budding" patented technology

The invention discloses a mushroom cultivating method utilizing residues of sophora flower buds after extraction of rutins. The mushroom cultivating method utilizing the residues of the sophora flower buds after the extraction of the rutins comprises processing burdening, namely weighing cultivation raw materials which comprise, based on the weight percent, cotton seed hull of 42-60%, the residues of the sophora flower buds after the extraction of the rutins of 28-40%, bran of 10-20%, gypsum of 0.6-2%, calcium superphosphate of 0.3-1%, carbamide of 0.2-1% and sugar of 0.8-2% and adding water into the cultivation raw materials after uniform mix of the cultivation raw materials to enable water content of the cultivation raw materials to achieve 55-65%; bagging and sterilizing after the burdening processing; inoculating and cultivating hypha; building a shed and earthing up; coloring; and managing fruiting. The mushroom cultivating method utilizing the residues of the sophora flower buds after the extraction of the rutins has the advantages of being simple in operation, capable of cultivating the mushroom by adding small amount of nutritional ingredients, quick in spawn runing, low in production cost and capable of effectively utilizing by-product residues in phytoextraction industries in a maximum limit and achieving sustainable development.

Construction systems for erecting budding structures comprise a plurality of prefabricated interconnectable modular budding units, each unit comprising framing members and a plurality of nodes, each node situated for selective interconnection with other units, the nodes and the exterior dimensions of the frame conforming to ISO shipping standards such that each unit is transportable using the ISO intermodal transportation system, and such that when the units are interconnected, a building structure is formed. The modular units are assembled at a remote location, and are there constructed to a semi-finished state, following which the semi-finished modular units are transported from the remote location to the job site, where they are secured to form the structure being erected, and the semi-finished modular units are thereafter constructed to a finished state.

The invention relates to a planting method of Chinese trichosanthes, belonging to the technical field of plantation and comprising the steps of: variety breeding, seedling reproduction, transplanting, field management, disease and pest control, harvesting and processing, wherein the variety breeding step includes the sub-steps of the tests in different production places, amplification and verification tests, resistance test, field pest investigation and trichosanthes nematode control; the seedling reproduction step includes the sub-steps of seed reproduction and root reproduction; the transplanting step includes the sub-steps of land selection and preparation, ridging, reproduction and planting, planting method implementation and field management; the land selection and preparation step includes the sub-steps of detection on chemical characteristic of soil, basic fertilizer application and disinfection; the reproduction and planting step includes the sub-steps of seed bud selection, rood cutting and breeding, planting and shed putting up; the planting method implementation step includes the sub-steps of planning density control and staminiferous plant matching; the filed management step includes the sub-steps of intertillagement and weeding, top application, shed putting up, pruning and cold prevention, in particular to intertillagement and weeding, root airing, intercrop, earthing, vine supporting and racking, top application, pruning, flower and fruit protection, chemical control and foliage application; and the disease and pest control step includes the sub-steps of control of diseases and pest and control method implementation.

The invention relates to a method for accurate fertilization for apple tree. The method decides target yield according to soil fertility based on 4000 kg of high quality organic fertilizer, then determine fertilizer consumption according needed fertilizer, fertilizer supply ability by soil and fertilizer availability, dressing all organic fertilizer, 70% of the nitrogen fertilizer and phosphatic fertilizer and 50% of potassium fertilizer as base fertilizer, taking left fertilizer for additional dressing, dressing the left 30% nitrogen before germination, dressing the left 30% phosphatic fertilizer before flower bud differentiation, dressing left potassium fertilizer during fruit expanding period, spraying fertilizer on leaf in different growing period for 6- 8 times in one year. The productivity and output value are increased by 21.5% and 21.3% respectively, the high- quality fruit rate is increased from 35% to 62%,and the net value is added by 615 yuan per mu.

Ear bud headphones configured for use with a portable media player include a main body portion with an extended curvature configuration. In one example implementation, the ear bud headphones include a speaker housing and an extension that form a gap between the speaker housing and the extension. A user can secure a portion of the user's outer ear in the gap (i.e., between the extension and speaker housing) such that the ear bud is securely and comfortably held in place within a user's ear. Additionally, the ear bud headphones can be interchangeable such that a user can customize the size, fit, and style of the ear bud headphone.

The invention discloses a method for preparing nano silver particles by using plant extract for reduction, and application of the method. In the method, the extract of natural plants such as chrysanthemum, honeysuckle flower, or bud of Japanese pagoda tree which serves as a reducing agent reacts with Ag<+> in aqueous solution of silver nitrate to ensure that Ag<+> is reduced into elementary substance silver nano particles. The method is simple, reaction conditions are mild, additives except for silver nitrate are not needed, the plant extract which is low in cost and can be easily obtained is used as the reducing agent, and the method is environment-friendly; the nano silver particles obtained by the method have smaller diameters, and high dispersibility and stability in aqueous solution; and Chinese herbal medicine components which have the effects of antibiosis and antiphlogosis are added, and the method has the characteristics of disinfection and sterilization, bacteriostasis, safety, no toxicity or irritation and the like and can be directly applied to multiple kinds of dermatological and surgical bacterial infection or medical equipment and cosmetics.

The invention belongs to the technical field of plant breeding, and in particular relates to a single flower selfing isolation method for small chili. The method comprises the following steps: (1) selecting an excellent single plant, namely in the flowering period, selecting the excellent single plant according to a breeding objective, and marking; (2) preparing a capsule shell, namely separating a shell cap from a shell body of a hollow hard capsule shell; (3) selecting a flower bud, namely selecting a large flower bud which is expected to bloom in the next day; (4) treating the flower bud, namely holding the flower bud to be treated by one hand, holding the capsule shell, which is matched with the flower bud in size, by the other hand, slightly sleeving the flower bud with the lower opening of the capsule shell over the widest part of the flower bud, and marking a flower stalk; (5) managing after the treatment in a method which is the same as a conventional field management method for the production of small chili. The capsule shell used in the method is low in cost and does not hurt the flower bud and the flower stalk when being used, the operation is convenient and easy, and the work efficiency is high; moreover, the capsule shell with certain elasticity and permeability does not influence the cracking of anther and pollination, and thus the selfing setting rate of the small chili is high.

The invention provides a method for propagating Oriusminutus artificially, which comprises the following steps: cultivation of spawning substrate, feeding of host; preparation of instruments of production, preparation of artificial feedstuff and preparation of seeds are carried out; under the conditions of 25 DEG C, 75% of PH and 16L:8D of illumination, kidney bean buds are taken as the spawning substrate, and Tetranychus cinnabarinus or the artificial feedstuff is used for feeding; firstly, the kidney bean buds on which the Oriusminutus spawn is produced are placed in a large tray, and kidney bean seedlings with proper Tetranychus cinnabarinus are placed in the large tray by interpenetration, according to the age period of Oriusminutus, the number of the proper Tetranychus cinnabarinus is confirmed; then the Oriusminutus with the tray are placed in an insect breeding cage for feeding the Oriusminutus. Spawn needs 12-16 days to grow to be imago, and the production period is about 30 days. The method is applicable to feeding with large amount and being promoted, thus laying the solid foundation for producing Oriusminutus with large amount and providing great convenience to biological control.

The invention discloses a selenium-enriched pleurotus eryngii planting method and a culture medium, comprising the following steps of confecting the culture medium, bagging, sterilizing, inoculating, fungus growing, bud hastening, producing mushroom and harvesting. The materials of the culture medium is manufactured according the weight portions of 70 to 90 portions of cotton seed shell, 8 to 10 portions of bran, 2 to 3 portions of corn meal, 0.6 to 1.2 portions of sucrose, 0.6 to 1.2 portions of gypsum, 4.5 to 6.5 portions of lime, 100 to 140 portions of water and 0.0011 to 0.0014 portion of sodium selenite. The method of the invention domesticates and cultivates the pleurotus eryngii seeds capability of absorbing and enriching selenium to ensure that the selenium-enriched pleurotus eryngii achieves the balanced production and is on the market all year, which improves the output and increases the economic benefit.

The invention discloses a green biological seedling raising matrix, relating to the seedling raising matrix technology. The preparation method comprises the following steps of: mixing straw powder, organic fertilizer and nutrient soil to be uniform, adding water until the water content is 45-55%, adding compound bacteria and then mixing to be uniform, stacking for fermenting, turning when the temperature increases to 50 +/- 2 DEG C, turning and cooling for 1-2 days when the temperature is increased to 50 DEG C again, adding microelements and growth hormone, stirring to be uniform, and packaging. The invention has the beneficial effects that: (1) the matrix is rich in organic matters and beneficial biological germs, has comprehensive nutrition and is loose and soft; (2) the matrix is seed-saving and labour-saving and can shorten seedling raising period; (3) the matrix can be used to ensure uniform and strong seedlings and high sprout rate; (4) the matrix can protect roots and hold water and fertilizer and has good air permeability; (5) the matrix has the functions of promoting flower bud differentiation and improving the yield; and (6) the matrix has the effect of resisting soil diseases. The matrix disclosed by the invention is applicable to seedling raising of vegetable, melon and fruit, flower and plant, tea, tobacco and cotton.

A security identification system is described that receives and processes multiple security credentials that have been configured by different network authenticators, devices configured to provide security credentials to user access devices, such as mobile phones and bracelets. The security identification system having multiple security certificates allows an employee to enter different locked physical facilities maintained by an employer when each of the locked physical facilities has been secured by a different security protocol, including security protocols provided by different security vendors. The security identification system may obtain security credentials over a network cloud service. The security identification system may take a variety of wearable forms, such as a bracelet, eyeglasses, rings, or ear buds, as well as forms such as a mobile phone. The security identification system may include a biometric reading device that measures users to verify that the person controlling an access device is its authorized user.

The invention discloses a network hiding method for a computer and a network hiding system based on the method. In the network hiding method, a passive processing strategy and an active processing strategy are adopted. The active processing strategy comprises the following steps of S1, when a detection behavior is ongoing according to judgment, replying false information to confuse a detector and increase the attacking difficulty; S2, faking false data traffic and a real host network behavior, and disturbing a sniffing behavior; and S3, dynamically and randomly jumping to hide a real host by taking the IP (Internet Protocol) address of the real host as a jumping element. In the network hiding system, four layers of network hiding models, including an access behavior control module, a dynamic multi-address module, a traffic confusing module and a protocol stack fingerprint confusing module are adopted. Due to the adoption of the method and system, unknown security problems can be blocked in advance, the occurrence of malicious network behaviors is blocked at a source, and advance protection is truly realized; due to the hiding characteristic, the aim of nipping in the bud can be fulfilled in the system; and the computer security is well ensured.

The invention relates to a preparation method of freeze-drying ginkgo fruit, which mainly comprises the following steps of: scalding gingkoes with testa removed; removing endotesta; subjecting the gingkoes to treatments of debitterizing, curing, secondary cooking in sugar, and sugaring; freezing and drying the gingkoes in vacuum to reach the water content of less than 3% and the sugar content of 9-15% in the product; and finally adopting deoxidized packaging to obtain a freeze-drying ginkgo fruit product. As the raw materials are subjected to the debitterizing, curing and color protection treatments in the invention, the bad bitter taste of internal buds in the gingkoes is overcome, the appearance, shape and color of gingko nutlet are kept unchanged, and the crispness and hardness of the product are increased. As the secondary cooking in sugar and the sugaring are performed, nutrient components of the product are increased, and the palatability of the product is improved; the water content is lower than 3% at the same time as the biological active components in the gingko nutlet are preserved to the maximum extent due to the freezing and drying of the gingkoes in vacuum; therefore, the preparation method is suitable for preserving the product; moreover, the freeze-drying ginkgo fruit is suitable for people to carry and have in leisure and a trip.

The invention discloses a method for propagating camellia japonica quickly, which comprises the following steps of: pruning branches of tea trees, and performing special processing; collecting cions of required varieties; and grafting, cutting and sealing tightly. Particularly, the method comprises the specific steps of: (1) collecting the branches; (2) collecting the cions; (3) grafting; (4) cutting; and (5) conveying the branches of the tea trees into a shed. The method has the advantages that: 1, the pruned branches of the wild tea trees can be grafted and cut immediately on the premise ofprotecting ecological resources, the branches, which are used as tree stocks, of the tea trees root, and the cions sprout buds within half a year and bloom in the second year, so the aim of shortening the marketing time by over 5 years is fulfilled under the condition of not taking 3 to 5 years for culturing the tree stocks into consideration; and 2, a section of pruned branch of the tea trees can root, sprout and bloom within two years by grafting and cutting; and the wild tea trees are not used as the tree stocks, so the populations of theaceae plants are protected effectively, ecology and society are facilitated, the cost is reduced, the economic benefit is improved, and the survival rate of cutting and grafting is over 85 percent.

The present invention discloses an apparatus for trimming plants, in particular, buds and flowers, to remove unwanted plant material. A typical application would be to process a plant to leave only trimmed buds and flowers for use in potpourri, fragrant sashes, or as a prelude to further processing of essential oils. The invention enables faster and safer production of plant material than prior plant processing machines. The apparatus employs an original combination of a slotted metal drum and a lawn mower type cutting reel that are rotated in the same direction, but at different speeds, to create a cutting interface that shears the unwanted plant material off and then collect this debris though a novel vacuum manifold into a waste collection device. The cutting interface between the slotted drum and the cutting reel is adjustable to keep the distance between the two between 0.002 and 0.006 inches for a very sharp and clean cut. The invention includes a roller brush and sprayer system to keep the cutting interface clean, without buildup of oils and other organic plant material. The invention is built upon a frame that moves on wheels and that allows for adjustment for operation in a level or tilted configuration to best suit the processing of particular types of plant material. The manifold cover is easily removed for cleaning and for sharpening or adjustment of the cutting blades.

A green health-care tea for decreasing blood fat and blood pressure, delaying senility and protecting liver and kidney is prepared from the male alabastrum of eucommia tree through screening, heat treating, cooling, kneading, protecting color, flavouring, cooling and loading in bags.

The invention relates to a high-efficiency breeding method of healthy seedlings of cane, the method is proposed aiming at the problems that seed performance degenerates, yield is greatly reduced and the quality is reduced, which are caused by that the cane is taken as asexually propagated crop, propagation coefficient is low, the amount of seeds used for cane implantation is large, and after the implantation of a plurality of years, the cane is easy to be infected with a disease; improved type healthy seedlings of the cane can be cultured by nine steps of seedling mild water detoxication processing, seedling sprouting, in vitro peeling inoculation, induction culture, virus check, propagation culture, rootage culture, domesticated seedling-refining and outdoor temporary planting; the method adopts the combination of mild water detoxication and stem tip detoxication with thorough detoxication and good effect; the direct peeling inoculation of the explant does not need to be treated with disinfection processing, has low pollution rate and light browning, and is easy to generate clustering buds by induction; the propagation coefficient is high, the speed is high, the period is short; the seed performance is table, the seedling is strong; the virus detection is precise and accurate; the survival rate of the outdoor temporary planting is high and is up to more than 90 percent; the production cost is low, the efficiency is high, and the method is suitable for industrialized production.

The invention relates to a Cas9 mediated carnation gene editing carrier and application. The application comprises the following steps: firstly, establishing a CRISPR-Cas9 system of carnation-containing target gene sites, introducing the Cas9 expression carrier into an agrobacterium tumefaciens C58 strain, putting roots of carnation leaves into a pre-culture medium, culturing with light for 3-4 days at 22+/-2 DEG C, activating agrobacterium containing the Cas 9 expression carrier, dipping the pre-cultured explant into the activated agrobacterium solution for 20-30 minutes, completely absorbing the agrobacterium solution, transferring into a co-culture medium, performing dark culture for 3-4 days at 22+/-2 DEG C, further transferring into a screening culture medium to culture, performing light culture at 22+/-2 DEG C so as to differentiate regeneration buds, further transferring the regeneration buds into a multiplication medium for multiplication screening culture, detecting positive transgenosis regeneration plants, sequencing target sites, and detecting mutation strain systems of carnation target gene sites.

The invention provides a method for performing mixed sowing of astragalus sinicus and oilseed rape and returning green manures to the field. The method comprises the following steps: a, sowing astragalus sinicus, namely sowing seeds of astragalus sinicus 25-35 days before harvesting of late rice, and keeping field water suitable for growth of astragalus sinicus; b, sowing oilseed rape, namely oversowing seeds of oilseed rape 1-3 days after harvesting of late rice; c, turning over and pressing the astragalus sinicus and the oilseed rape, namely when the astragalus sinicus is in a full-blossom period and the oilseed rape is in a bud period, simultaneously turning over and retting two types of green manures about 15 days before planting of early rice, wherein routine ploughing, fertilizing and early rice transplanting can be performed after retting is performed for 8-10 days. The method provided by the invention can be used for solving the problems of single planting variety and single planting structure in production and utilization of green manures in the southern double-crop rice region, effectively solving the problems that green manure nutrients are imbalanced after uni-sowed Chinese milkvetch or oilseed rape is turned over and pressed and thus nutrient supply can not meet the requirements of the whole early rice growth period on the nutrients, and the like, being beneficial to the improvement on the yield and quality of aftercrop early rice and playing a role in directly promoting the green manure development re-arisen in China.

The invention discloses a method for trapping tea plantation insects comprising the steps of, (1) separating and determining 10 pest damage volatile matters by adopting adsorptive separation method, (2) determining four volatile constituents with inducement activity by biological assay method, (3) preparing four active constituents into flavor source substances, matching the four flavor source substances, so as to formulate the insect attractant, (4) determining three colors with inducement effect to insects by tea plantation small zone inducement experiments, (5) combining the bud greening color with the attractant to prepare insect trapper, and (6) placing the trapper into the field to trap the insects.

The invention relates to a propagation method for using a single-bud short tamarix to cut containerized seedlings. The propagation method comprises the main operating steps of: firstly establishing pergolas, preparing cutting containers, cutting matrixes and cutting beds of the containers, then carrying out cutting propagation operation, selecting the branches of the middle-upper part of the periphery of the middle-old age excellent clonal crown as cuttings, carrying out cutting in summer or autumn, doing well in work of weeding, fertilizing, moisturizing and prevention of pests. The propagation method has short culturing period, generally needs only 10 to 12 months, has simple operation, high propagation coefficients, fast propagation expansion and good transplanting effect, also can effectively reduce the loss of cutting propagation materials, and improve comprehensive benefit of industrial development of good seeds of the oil tea.

The invention provides a Chinese medicinal composition for treating haemorrhoids and a preparation method thereof. The Chinese medicinal composition is prepared from pangolin scales, rehmannia root, carbonized human hair, fortune windmillpalm petiole, papaya, dahurian angelica root, hedgehog hide, stone-like pmphalia, szechuan lovage rhizome, Chinese angelica, peach seed, golden thread, cochinchina momordica seed, poppy shell, ground beeltle, Japanese ampelopsis root, amur corktree bark, rhubarb, lightyellow sophora root, Chinese gall, dark plum fruit, rhizoma atractylodis, red paeony root, baical skullcap root, figwort flower picrorhiza rhizome, gardenia, pagodatree pod, Chinese lobelia herb, safflower, honeysuckle flower, pagodatree flower bud, frankincense, dragon blood, myrrh, borneol and sinkiang arnebia root. The medicament of the invention is a medicinal preparation prepared from the extracts of the raw material medicaments. The medicament of the invention has the effects of promoting blood circulation, dispersing swelling, removing blood stasis, relieving pains, detoxifying, draining pus, eliminating putridity and engendering flesh and is mainly used for treating light water and fire burns, swelling and pain of sores and wound fester.

The invention discloses a trap method for Empoasca vitis Gothe imago,nympha and spiny white fly imago, comprising the steps: 1)separating and authenticating 9 volatile matter from sturdy tea twig using adsorption method 2) determining three volatile ingredient with allure activity using biological determination method 3)respectively preparing three active ingredient, concentration 0.01-20mu g/ml, mixing according to 1:0.2-1.5:0.1-1, preparing attractant, 4)determining three colors with alluring effect to Empoasca vitis Gothe and spiny white fly from ten colors in the tea garden allure experiment, three colors being parrot green, bud green and jasmine yellow 5) combining attractant with bud green and jasmine yellow, making trapper for Empoasca vitis Gothe and spiny white fly The advantage of the invention : 1)no pollution, adapting to organic tea and nuisance free tea production, 2) low cost.

An electronic device cover provides a protective cover for an electronic device and also extends and retracts a plurality of audio and data transfer modules at varying speeds and lengths. The electronic device cover is configured and shaped to receive myriad types and models of electronic devices. A proximal end of a communication cord extends from the electronic device cover to provide communication modules such as ear buds, or head phones to a user. A distal, shorter end of the same cord extends more slowly and at a slower speed from a different end of the electronic device cover to provide connections to the more proximal electronic device, power sources, and other electronic ports. The cords extend at different speeds and lengths due to a differential affect.

The invention relates to an ecological compound planting method of Paris polyphylla, belonging to the technical field of plant cultivation. The invention utilizes that principle of the stratification of different plants and ecological niche space complementation, adopts the compound planting mode of spruce or Alnus nepalensis+ apple+ Paris polyphylla to improve the survival rate of Paris polyphylla and lower planting cost. The specific steps are as follows: after artificially planting spruce forests or Alnus nepalensis forests for two years, planting Amomum tsao-ko; after planting for one year, developing the ecological compound planting of the Paris polyphylla by the natural shade and water retention effect formed by an artificial forest and apples; reasonable management of a seedling stage and modes, such as reasonable fertilization, weeding, topping, bud picking and the like are utilized to realize the ecological compound planting of the Paris polyphylla. The invention has the advantages of simple operation, low cost, high benefit, small risk, land and labor saving, environment protection, easy popularization and high economic benefit, sets a good basis for artificial scale planting of the Paris polyphylla, has favourable economic benefit, social benefit and ecological benefit.

The fast tissue culture propagation process for azalea and camellia includes the following steps: cutting tender stem segment from the maternal plant in April or May; soaking in alcohol solution, sterilizing in HgCl2 solution and cutting the stem tip; inoculating the stem tip in primary culture medium to grow cespitose bud and cutting single bud seedling after the bud grows to 2-3cm length; culturing the single bud seedling in secondary culture medium inside a sealed container to proliferate test tube seedling repeatedly; culturing the test tube seedling in rooting culture medium inside sealed container to grow new root to 0.3-0.5cm long, and transplanting. The process can provide seedling of azalea and camellia continuously.

The invention relates to a rapid tree micrografting propagation method. The method comprises the following steps: (1) selecting two tree twigs with good affinity or twigs of the current year on a tree as rootstocks and scions respectively; (2) respectively performing sterilization treatment on the rootstocks and the scions; (3) respectively performing hormone treatment on the rootstocks and the scions; (4) grafting the rootstocks and the scions in the way of scion grafting or bud grafting; (5) cultivating grafted seedlings in a sand bed; (6) removing rootstock shoots in time; and (7) removing binding plastics after the scion survives and young sprouts grow to 20-30cm, thus completing rapid micrografting propagation of the rootstocks and the scions. The method applies the modern biotechnologies to fine variety breeding, realizes industrial large-scale seedling culture, and the method can be implemented in protected areas all the year round. Compared with the existing grafting seedling culture methods, the propagation method has the advantages of low cost, fast seedling formation, robust growth of nursery stocks and easy transplantation, and can rapidly propagate to obtain great amount of new species of good nursery stocks in a short time.

An apparatus and method for retaining an ear-bud, said apparatus having a base, first and second sidewalls at opposing ends of the base forming a receptacle, a flexible member stretched around the first and second sidewalls of the base, wherein the flexible member forms a band that stretches and contracts to enable the apparatus to retain ear-buds having various configurations. The sidewalls are preferably attached to a portion of the periphery of the base, which is preferably an elongated, flat platform. The flexible member forms a band that is stretched around the sidewalls, wherein a portion of the flexible band is not connected to the sidewalls and is capable of expanding and contracting so as to receive and retain an ear-bud placed within the receptacle.

The present invention relates generally to a trimming machine used to cut flowers or other plant material into small sized fragments or to prune unwanted thorns or leaves off of plants and flowers. This machine trims flower petals into small pieces which can be used in potpourri or other displays. This machine enables the user to generate a greater volume of flower petals then the traditional method of hand trimming with a scissors. This machine can also trim the unwanted leaves, buds or thorns off plants and flowers.

The invention provides a preparation method of virus-free seedlings of sweet potato. The method comprises the following steps: placing sweet potato blocks that are exposed under the sun and matured banana together for 3 to 5 days; planting the sweet potatoes in sandy soil to accelerate the germination in a culture box; setting relatively high temperature; picking 1-2cm top end of the seedling based on the length; cutting off the visual leaves; disinfecting the surface; rinsing with sterile water; picking off stem tip with 1 to 2 leaf primordium; inoculating to the corresponding culture medium to respectively induce differentiation of adventitious buds and generation of somatic embryogenesis; performing virus detection to a regeneration plant; performing subculture for the virus-free seedling; hardening-seedling; directly planting into a plastic greenhouse; performing flood irrigation with more water; preventing direct exposing under sun at noon in the first two weeks after transplanting; applying urea three weeks after transplanting; watering to obtain virus-free seedlings. According to the preparation method of the virus-free seedlings of sweet potato, the virus-free test-tube plantlets are acclimated and then directly planted into the plastic greenhouse, thus the survival rate is obviously raised, and the operation processes are decreased; the process of acclimating in an acclimating room is saved, and as a result, labor, materials and property are saved.