215results about How to "High genetic stability" patented technology

The present invention provides recombination based methods for assembling nucleic acids. In certain aspects the present invention provides hierarchical assembly methods for producing genome sized polynucleotide constructs. The methods may be used for assembling large polynucleotide constructs, for synthesizing synthetic genomes, or for introducing a plurality of nucleotide changes throughout the genome of an organism. In another aspect, the invention provides cells having increased genomic stability. For example, cells comprising alterations in at least a substantial portion of the transposons in the genome are provided.

The invention discloses gene recombination bacillus amyloliquefaciens and a microbial inoculum preparation method. A bacillus amyloliquefaciens FZB42 strain is taken as a host bacterium to construct the gene recombination bacillus amyloliquefaciens XLV09-1 with transparent vitreoscilla hemoglobin which is regulated and controlled by a P43 strong promoter by contrasting a gene recombination vector. The produced spore number of microbial inoculum prepared by fermenting the bacillus amyloliquefaciens can reach more than 100 hundred millions per milliliter, the overall yield of antibacterial lipopeptid reaches more than 1 gram per liter and prevention effect on various fungal plant diseases is improved by over 30 percent. The microbial inoculum is a green microecological preparation, of no toxic residue, safe to human and livestock, and favorable for protecting the ecological environment.

Disclosed is the high quality germchit tissure culturing and rapid breeding method of Dendrobium sp., wherein a large amount of test tube seedlings with stabilized performance are obtained through fine breed tissue culture by adopting the path of clumping buds. The produced Dendrobium sp. has the advantages of high genetic stability, quick emergence of seedlings, strong seedlings, good seedling quality, strong resistance, and fine growth status.

The present application relates to arenaviruses with rearrangements of their open reading frames (“ORF”) in their genomes. In particular, described herein is a modified arenavirus genomic segment, wherein the arenavirus genomic segment is engineered to carry a viral ORF in a position other than the wild-type position of the ORF. Also described herein are trisegmented arenavirus particles comprising one L segment and two S segments or two L segments and one S segment. The arenavirus, described herein may be suitable for vaccines and/or treatment of diseases and/or for the use in immunotherapies.

A genotype-independent method for efficiently carrying out pollen-mediated transformation of maize, tomato or melon is described. The method uses pollen pretreated with silicon carbide and DNA to produce transformed plants with high efficiency and reproducibility.

The invention discloses a method for breeding microorganisms by plasma-induced mutation, which radiates microorganisms to be induced to mutate with a plasma jet flow that uses neutral active particles as acting particles to obtain mutated microorganisms. The method in which the neutral active particles in the plasma jet flow play a main induction role has the advantages of: ensuring the high activity of forward mutant strains and the high capability and genetic stability of produced target products, reducing mutagenesis period and improving mutagenesis efficiency, inducing a great variety of microorganisms such as prokaryotic microorganisms, eukaryotic microorganisms and archaebacteria in form of microbial community, bacterial suspension or spore suspension, simplifying experimental operation by avoiding culturing the processed microorganisms in the dark, having the characteristics of simple, safe and pollution-free whole operation, low equipment and experiment cost and the like and having great potential role in the field of industrial microorganism induced mutation breeding.

Long term calorie restriction has the benefit of increasing life span. Methods to screen interventions that mimic the effects of calorie restriction are disclosed. Extensive analysis of genes for which expression is statistically different between control and calorie restricted animals has demonstrated that specific genes are preferentially expressed during calorie restriction. Screening for interventions which produce the same expression profile will provide interventions that increase life span. In a further aspect, it has been discovered that test animals on a calorie restricted diet for a relatively short time have a similar gene expression profile to test animals which have been on a long term calorie restricted diet.

The invention discloses a cutting propagation method for splash-ink dendrobe old stems. The method comprises following steps: utilizing two-year or perennial splash-ink dendrobe pseudobulb as material; utilizing a high-temperature high-humidity condition for drought stress, forming buds induced on old stems, carrying out final-period management and seedling transplant to culture new excellent parental plant. The method is utilized for obtaining cuttage seedlings with excellent parental characters. From March to May, seedlings grow. From October to December, flowers bloom. New stem sprouts germinate for 12 to 25 days. Each pseudobulb has 1 to 4 buds of more than 90% germination rate. Compared with the aseptic sowing and tissue culture propagation, the method has following beneficial effects: the method is stable in inheritance, short in culture period, fast in florescence, low in production cost, easy in operation and easy in promotion; new plants can be cultured by effective utilization of aging pseudobulb so that nutrient consumption of new pseudobulb by utilizing aging pseudobulb is reduced and service lifetime of seedlings is prolonged; and therefore, a new way is provided for splash-ink dendrobe seedling propagation and effective utilization of old stems.

The invention relates to plant tissue culture and discloses a tissue culture method for Vaccinium bracteatum Thunb, belonging to the field of plant cultivation. The method comprises the following four steps: explant sterilization; induction culture; propagation culture; and rooting culture. The method has the advantages of a high propagation rate, a fast growth rate and high hereditary stability, overcomes the problem of difficult breeding of Vaccinium bracteatum Thunb, can realize large-scale production and industrial large-scale supply of finished seedlings, protects excellent wild plant resources and brings about good economic values.

The invention provides a technology for inducing the redifferentiation of calluses of Taxus wallichiana var. mairei to improve or maintain the genetic stability of the cellular metabolism of the Taxus wallichiana var. mairei and improve the content of paclitaxel as a secondary metabolite of the Taxus wallichiana var. mairei. The technology comprises four steps of explant preprocessing, induced culture of calluses, calluses subculture and calluses redifferentiation induction. In the step of induced culture of calluses, subculture is performed four times with 20-35 days for each culture, the calluses with good color and right texture are transferred to an improved MS (Murashige & Skoog) culture medium in which 0.05-0.20mg/L6 of BA, 0.2-2.0mg/L of NAA, 0.1mg/L of KT and 0.5mg/L of CH are added for redifferentiation induction culture, after 45-75 days of the induction culture, tiny sprouts grow from the calluses of the Taxus wallichiana var. mairei, the calluses are harvested at the time, after samples are subjected to drying, weighing and a series of preprocessing, HPLC (High Performance Liquid Chromatography) is adopted to measure the content of the paclitaxel, and a result shows that the content of the paclitaxel is one time higher than that of the calluses which are not subjected to differentiation. In the improved MS culture medium, ammoniacal nitrogen is 2000-2600 mg/L and the nitrate nitrogen is 800-1000mg/L.

The invention discloses a Paenibacillus sp. PN-S435, with the preservation number: CCTCC M 2012422, microcystins (MCs) are the only carbon source and nitrogen source, and MCs can be effectively degraded. The Paenibacillus sp. PN-S435 can be obtained through the performing combination of compound mutation of low-energy N+-LiCl with compound mutation of UV-LiCl for original strain PV-3, and then performing preliminary screening and secondary screening. According to the invention, the capability of the strain degrading MCs reaches 30.2 mg/L per day, which is improved by 64.1percent compared with original strain; and the strain greatly improves the capability of degrading MCs in a water body, hereditary stability tests prove that the Paenibacillus sp. PN-S435 can be applied to practical application, controls water body with serious algal bloom, removes even avoids danger of algal toxin to drinking water safety.

The invention discloses recombinant pseudomonas putida, which is expressed on the basis of DNA integration. The invention also discloses a construction method for the recombinant pseudomonas putida, and the application of the recombinant pseudomonas putida in phosphorus removal from wastewater. The method of the invention is simple, and obtained genetically engineered bacteria have high-efficiency phosphorus removal capacity.

The invention provides a polymorphic hansenula polymorpha mutant strain and application of the polymorphic hansenula polymorpha mutant strain in glutathione biosynthesis. Firstly, low-energy ions N+ serve as a mutagenesis source, mutagenesis treatment for Hansenula polymorpha DL-1 is achieved, a glutathione (GSH) high-producing strain (DL-18) is obtained, the temperature remains 37 DEG C, the speed remains 180 r/min, shake flask fermentation remains for 42 hours, the dry cell weight (DCW) in end products is 7.04 g/L, the total quantity of the GSH is 420.46 mg/L, and the DCW in the end products and the total quantity of the GSH are respectively improved by 1.73 times and 1.94 times compared with the original mutant strain. Hereditary stability of the DL-18 is detected through subculture, yatsushiro is cultivated, content changes of the GSH are not significant, and therefore, the hereditary stability of the mutant strain is high.

The invention relates to a recombination strain for producing trans-4-hydroxy-L-proline and building and application of the recombination strain and belongs to the technical field of genetic engineering. The recombination strain for producing the trans-4-hydroxy-L-proline is identified as escherichia coli HJ which is preserved in China Center for Type Culture Collection on September 17th, 2015, and the preservation number of the escherichia coli HJ is CCTCC No. M2015550. The recombination strain has the advantages that wild glutamate kinase controlled by feedback regulation is used to enhance expression, three to-be-expressed genes and expression elements such as related initiators are integrated to the chromosome of the escherichia coli, the to-be-expressed genes can be constantly replicated along with the replication of the chromosome, extremely-high genetic stability is kept, the problem of plasmid loss is solved, effective fermentation cycle is prolonged, and the yield of hydroxyproline is increased.

Recombinant human parainfluenza virus type 2 (HPIV2) viruses and related immunogenic compositions and methods are provided. The recombinant HPIV2 viruses, including HPIV2 chimeric and chimeric vector viruses, provided according to the invention are infectious and attenuated in permissive mammalian subjects, including humans, and are useful in immunogenic compositions for eliciting an immune responses against one or more PIVs, against one or more non-PIV pathogens, or against a PIV and a non-PIV pathogen. Also provided are isolated polynucleotide molecules and vectors incorporating a recombinant HPIV2 genome or antigenome.

The invention discloses a rapid propagation method for plateaus of alliums. The method comprises the following steps: cleaning and disinfecting central parts of the alliums; transversely cutting the central parts 3-5 millimeters away from bottom ends to remove upper scales; longitudinally partitioning into four parts along a vertical tangent line of a main growth point; removing the main growth point and the plateau where the main growth point is positioned; inducing multiple shoots; culturing into seedlings. Through optimization of the culturing process, a rapid propagation system for the plateaus of the alliums is established and a large quantity of virus-free tube seedlings are obtained. The system has the advantages of short proliferation time, large proliferation coefficient and high genetic stability. Through the system, rapid propagation of the alliums can be realized. Industrialized production of allium virus-free tube seedlings can be ensured with a low price cost and a low time cost, and development of the allium industry is promoted.