215results about How to "High genetic stability" patented technology

The invention discloses a watermelon tissue culture seedling grafting method, comprising the following steps: stock preparation, scion preparation, grafting, grafted seedling culturing, grafted seedling transpiration and the like. Stock seeds are sowed in a nutrition disc after pregermination, and can be grafted after two leaves and one bud grow; 2-3cm-high watermelon tissue culture seedlings are domesticated and subjected to scion ingraftment, and grafted through a top grafting method; the grafted seedlings are subjected to timely heat preservation, humidity preservation, shading culturing, and shoots of stock are timely removed; and the grafted seedlings with two leaves and one bud can be transplanted into the land for growing field crops and subjected to field management as same as common grafted seedlings. According to the invention, rooting is avoided in the tissue culture seeding culturing, the problem of difficulty in rooting or instability can be overcome by directly obtaining the watermelon grafted seedlings; the method can be carried out all the year round with artificially controlling conditions, is short in period, high in rate of survival, simple and feasible and convenient to operate, the production cost of the watermelon grafted seedlings is reduced, and the problems of imperfect regeneration plant rooting effect and low transportation survival rate during watermelon tissue culturing are solved.

The disclosure provides for an isolated recombinant influenza virus having at least one of: a PA gene segment encoding PA with a residue at position 443 that is not arginine, a PB1 gene segment encoding PB1 with a residue at position 737 that is not lysine, a PB2 gene segment encoding PB2 with a residue at position 25 that is not valine or a residue at position 712 that is not glutamic acid, a NS gene segment encoding a NS1 with a residue at position 167 that is not proline, a HA gene segment encoding a HA with a residue at position 380 that is not threonine, or any combination thereof, and methods of making and using the virus.

The teachings herein are generally directed to a method of enhancing the genetic stability of parvovirus vectors. The stability of conventional ss or dsAAV vector constructs can be enhanced, for example, to obtain a concurrent increase in vector titer and purity, as well as an improvement in vector safety, due at least in part to the elimination of stuffer DNA from the AAV vector. The method is broadly applicable to all gene transfer / therapy applications, such as those requiring delivery of foreign DNA containing recombinant gene expression cassettes. Such foreign DNA can range, for example, from about 0.2 up to about 5.2 kb in length. The enhanced vector constructs are highly flexible, user-friendly, and can be easily modified (via routine DNA cloning) and utilized (via standard AAV vector technology) by anyone skilled in the art.

In the invention, apical bud of zephyr lily bulb is selected for fast inducing to produce a large number adventitious buds, regeneration plant is obtained by generation and rooting of the adventitious buds, survival percentage of the regeneration plant reaches 100%; in addition, the method of the invention has the advantages of fast propagation speed, no confinement to seasons, acquisition of apical bud isolated organ at any time for tissue culture, small mutation of the regeneration plant, high genetic stability, healthy regeneration plant and shortened seedling period.