Watermelon tissue culture seedling grafting method

Abstract

The invention discloses a watermelon tissue culture seedling grafting method, comprising the following steps: stock preparation, scion preparation, grafting, grafted seedling culturing, grafted seedling transpiration and the like. Stock seeds are sowed in a nutrition disc after pregermination, and can be grafted after two leaves and one bud grow; 2-3cm-high watermelon tissue culture seedlings are domesticated and subjected to scion ingraftment, and grafted through a top grafting method; the grafted seedlings are subjected to timely heat preservation, humidity preservation, shading culturing, and shoots of stock are timely removed; and the grafted seedlings with two leaves and one bud can be transplanted into the land for growing field crops and subjected to field management as same as common grafted seedlings. According to the invention, rooting is avoided in the tissue culture seeding culturing, the problem of difficulty in rooting or instability can be overcome by directly obtaining the watermelon grafted seedlings; the method can be carried out all the year round with artificially controlling conditions, is short in period, high in rate of survival, simple and feasible and convenient to operate, the production cost of the watermelon grafted seedlings is reduced, and the problems of imperfect regeneration plant rooting effect and low transportation survival rate during watermelon tissue culturing are solved.

Description

technical field [0001] The invention relates to the technical field of grafting, and more specifically relates to a method for grafting watermelon tissue culture seedlings. The cost is low, and the survival rate of grafting is high. Background technique [0002] watermelon[ Citrullus Lanatus (Thunb.) Matsum. et Nakai] Cucurbitaceae, watermelon genus, annual vine herb. Originating in Africa, it is one of the five largest fruits in the world and occupies an important position in my country's national economy. Grafting is the only effective way to solve soil-borne diseases such as watermelon wilt at present. Although the watermelon seed grafted seedlings in production can be produced and promoted on a large scale, the cost is relatively high for seedless watermelons and varieties with expensive seeds. At the same time, although watermelon tissue culture seedlings have more advantages in multiplication coefficient, propagation speed and quantity, and can reduce the production...

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of watermelon tissue culture seedling grafting method, take the watermelon tissue culture seedling as the scion, take the gourd or the pumpkin seedling as the grafting technique of the rootstock, Solve the current problems of rooting and transplanting of regenerated plants in watermelon tissue culture, make watermelon tissue culture seedlings used for grafted seedling production, and provide technical guarantee for watermelon grafting-related theoretical research, variety improvement, cell engineering, etc.