59 results about "WATERMELON SEED" patented technology

The invention discloses a molecular marker, a primer pair, a kit and a method for identifying the size character of watermelon seeds and application, and belongs to the technical field of molecular marker-assisted breeding. The invention provides a molecular marker (as shown in SEQ ID NO.1-2), a primer pair (as shown in SEQ ID NO.3-4) and a kit containing the primer pair for identifying the size character of watermelon seeds in order to identify the size character of the watermelon seeds more quickly and more accurately in the watermelon seedling stage and realize watermelon germplasm resource screening and molecular assisted selection breeding. According to the identification method provided by the invention, the to-be-detected watermelon genome DNA is extracted in the seedling stage, so that molecular marker-assisted selection can be performed on the watermelon seed size character, the operation is simple and convenient, the breeding accuracy and efficiency are improved, the variety breeding period is shortened, and the variety breeding process is accelerated.

The invention discloses a watermelon 2.7 K liquid phase breeding chip as well as a special single nucleotide polymorphism variation site and application thereof. The 2.7 K liquid phase breeding chip provided by the invention comprises a probe combination for detecting 2765 single nucleotide polymorphism variation sites in a watermelon genome, and the position information of a probe corresponding to each single nucleotide polymorphism variation site in a watermelon reference genome is shown in a table 3 in the specification. Single nucleotide polymorphism variation sites involved in the liquid phase breeding chip provided by the invention have the advantages of high polymorphism, good repeatability, stable and reliable marking, convenient statistics and the like, and a DNA fingerprint database for identifying the authenticity of watermelon varieties can be established based on the liquid phase breeding chip. The method can be used for authenticity of watermelon varieties, early breeding and identification of the watermelon varieties and identification of watermelon seed resources, provides technical support for enrichment of watermelon germplasm resources, breeding of new varieties and protection of the new varieties, and has a wide application prospect.

The utility model discloses a watermelon seed cultivation box which comprises a box body, spray pipes distributed up and down are rotatably connected in the box body, the outer walls of the bottoms of the spray pipes are connected with nozzles evenly distributed through threads, a connecting shaft at one end of each spray pipe extends to the outside of the box body and is fixedly provided with a gear, and the outer wall of one side of the box body is slidably connected with a rack. A motor is fixed to the outer wall of the top of the box body, a rotating disc is fixed to an output shaft of the motor, a connecting column is fixed to the outer wall of one side of the rotating disc, and a connecting rope is hinged between the connecting column and the rack. According to the watermelon seed water supplementing device, the motor drives the rotating disc to rotate, the rotating disc pulls the rack through the connecting rope, then the rack drives the gear and the spraying pipe to shake, the area of water mist sprayed by the nozzle is increased, and watermelon seeds in the tray are more uniform during water supplementing.

The utility model relates to the field of seed processing, and discloses a watermelon seed drying device which comprises a drying box, a plurality of supporting rods are fixedly connected to the two inner side walls of the drying box, a supporting plate is connected between every two opposite supporting rods in a clamped mode, and a containing box is fixedly connected to the upper surface of each supporting plate; a plurality of moving plates are slidably connected to the inner wall of the drying box, rotating columns are rotatably connected to the lower surfaces of the moving plates, the moving plates are rotatably connected with turnover devices through the rotating columns, spraying plates are fixedly connected to the inner side wall of the drying box, and an air heater is fixedly connected to the side surface of the drying box. A conveying pipe is fixedly connected to the upper surface of the air heater, and the output end of the conveying pipe penetrates through the drying box and then is fixedly connected to the side surface of a driving spraying plate; according to the watermelon seed drying device, watermelon seed rows can be automatically turned over in the drying process, uneven heating of seeds is avoided, and the drying uniformity is improved.

This invention provides a traditional Chinese medicine composition and its application, belonging to the field of biomedical technology. The traditional Chinese medicine composition includes chicory, Tian Shan violet, fennel, rhubarb, dodder seed, chicory seed, ox tongue grass, cucumber seed, watermelon seed, rose petals, and mare's milk raisins. This composition is a targeted, dialectical formula for clearing the liver and promoting bile secretion. Its core advantage lies in its precise alignment with the physical characteristics of people in southern Xinjiang. Through the clever combination of four distinctive ingredients—cucumber seed, watermelon seed, rose petals, and mare's milk raisins—it achieves a high degree of compatibility between efficacy and constitution. This composition adheres to the core efficacy of clearing the liver and promoting bile secretion while also addressing the needs of people in southern Xinjiang for moisturizing, regulating qi, and protecting the spleen and stomach. This precise match between efficacy and constitution makes it a high-quality formula suitable for the people of southern Xinjiang and highly practical.

The present invention discloses the role and application of the ClAIP1 gene in regulating male sterility in watermelon, belonging to the technical field of watermelon molecular breeding. This invention discloses for the first time the function of the ClAIP1 gene in regulating male sterility in watermelon. Transgenic plants were obtained by knocking out the gene using the CRISPR / Cas9 method. The anthers of the male flowers of the transgenic plants were significantly degenerated, with no visible pollen production or shedding. The female flowers and vegetative organs showed no significant changes compared to wild-type plants, resulting in completely male-sterile germplasm. The provided ClAIP1-knockout transgenic plants / mutants are novel male-sterile watermelon mutants, with normal female flowers and vegetative organs. These mutants can be used as new male-sterile watermelon germplasm, contributing to simplifying the watermelon seed production / variety selection process, shortening the seed production / breeding period, improving hybrid purity, and reducing seed production / breeding costs. The invention has significant production and application potential.

The invention provides a high-throughput sorting system for watermelon seed purity. The high-throughput sorting system comprises a rack, an analysis control mechanism, a feeding mechanism, a near-infrared spectrum collecting mechanism and a sorting and seed collecting mechanism, wherein the feeding mechanism, the near-infrared spectrum collecting mechanism and the sorting and seed collecting mechanism are arranged on the rack; the feeding mechanism is used for conveying to-be-detected seeds to the near-infrared spectrum acquisition mechanism in a branching manner, the near-infrared spectrum acquisition mechanism is used for acquiring diffuse reflection spectrum data of the seeds in a full-surrounding light environment and transmitting the diffuse reflection spectrum data to the analysis control mechanism, and the analysis control mechanism analyzes information of single seeds according to the received data and classifies the single seeds; the sorting and seed collecting mechanism sorts the seeds according to the classification of the analysis control mechanism and collects the seeds into corresponding seed collecting trays; the invention provides a watermelon seed genotype sorting scheme which is nondestructive, high in throughput and high in precision, the seeds conforming to the target genotype are sorted out by automatically collecting and extracting phenotype data and characteristics of watermelon seeds and constructing a model to analyze the genotype, and the seed purity detection and sorting precision and efficiency are high.

The utility model relates to the technical field of food processing, in particular to a storage device for watermelon seed kernel production, which comprises a box body and further comprises a mounting frame arranged on the inner wall of the box body, the inner wall of the mounting frame is connected with a supporting plate in a sliding manner, and a storage box for storing watermelon seed kernels is arranged at the top of the supporting plate; the guide block is pulled to slide in the guide rail through movement of the guide rail, the connecting plate and the mounting frame are driven to rotate, watermelon seeds in the storage box can be conveniently poured out subsequently, the watermelon seeds can be conveniently taken out for processing subsequently, and the problem that subsequent processing efficiency is affected by pipeline discharging is solved; the inside of the box body is refrigerated through the air outlet pipe and the air outlet covers, the problem that the watermelon seed kernels go bad or generate bad flavor due to the high temperature is solved, the multiple sets of air outlet covers are arranged, the effect of refrigerating the inside of the box body at different heights is achieved, and the temperature uniformity in the box body is guaranteed.

The utility model relates to the technical field of food processing, in particular to a dust removal feeding device of a color sorter for watermelon seed kernels, which comprises a support and further comprises a support frame arranged on one side of the support, the top of the support frame is fixedly connected with a storage box, a blanking frame is mounted on the top of the storage box, a guide frame is mounted on the top of the blanking frame, and a dust removal device is mounted on the guide frame. The filter screen can be conveniently detached by arranging the detaching mechanism, dust and impurities can be gradually accumulated in the use process of the filter screen, the filter screen can be ensured to continuously and efficiently operate by regularly detaching and cleaning the filter screen, blockage and reduction of dust removal efficiency are prevented, meanwhile, the filter screen convenient to detach is also convenient to carry out necessary inspection and maintenance, and the service life of the filter screen is prolonged. The service life of the equipment is prolonged, and the filter screens of different specifications or materials can be replaced by a user according to actual requirements through the filter screens convenient to disassemble so as to adapt to changes of different materials or production requirements.

The utility model relates to a special culture device for watermelon planting, which is characterized in that hollow frames are vertically and fixedly mounted in the middles of the two ends of the outer side of a box body, sliding plates are slidably mounted on the surfaces of the two ends of the outer side of each hollow frame, a containing frame is slidably mounted in the box body, a lifting mechanism is arranged in each hollow frame, and the lifting mechanisms are in transmission connection with the sliding plates; driven mechanisms are arranged on the two sides of the interior of the box body, a transmission frame is fixedly installed on the outer side of each sliding plate, a connecting plate is fixedly installed between the bottoms of every two sets of transmission frames, a transmission mechanism is arranged on the side edge of each connecting plate, and the transmission mechanisms are in transmission connection with the outer side of the containing frame; according to the watermelon seed collecting device, watermelon seeds in the containing frame can roll and gather together under the action of the gravity of the watermelon seeds, so that an operator can conveniently move and collect the watermelon seeds, and meanwhile, damage to the skin of the watermelon seeds caused by excessive contact between the watermelon seeds and a germination accelerating agent when the watermelon seeds are moved is avoided.

This invention provides a method and apparatus for extracting watermelon seeds, relating to the field of agricultural technology. The watermelon seed extraction apparatus includes a peeling box, a first conveyor belt assembly, a first centrifuge, a second conveyor belt assembly, a second centrifuge, a third conveyor belt assembly, and a fourth conveyor belt assembly. In this invention, the watermelon is enveloped by two sets of relatively rotating segments within the peeling box, causing the watermelon to rotate randomly. During this rotation, the watermelon continuously contacts the inner wall of the segments, allowing the peel to be quickly removed by the peeling blade. This avoids the problem of watermelon peel mixing in and being difficult to separate during the subsequent seed extraction process. The peeled pulp is then cut by a cutting blade during transport and further centrifuged and pressed by the first centrifuge, avoiding the presence of large pieces of pulp and greatly improving the separation efficiency of the second centrifuge. The pulp, juice, and seeds are thoroughly separated, resulting in excellent separation performance.

1. Name of the product in this design: Watermelon Spoon (Duck). 2. Purpose of this design: for scooping out watermelons and picking out watermelon seeds. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention discloses a liver-protecting traditional Chinese medicine composition as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The liver-protecting traditional Chinese medicine composition comprises a traditional Chinese medicine aqueous extract and fruit vinegar, and the traditional Chinese medicine comprises the following components in parts by weight: 2-10 parts of cichorium glandulosum seeds, 10-20 parts of fennel, 2-10 parts of celery seeds, 2-10 parts of white rapeseeds, 2-10 parts of melon seeds, 2-10 parts of watermelon seeds, 2-10 parts of cucumber seeds, 2-10 parts of purslane seeds and 10-20 parts of cichorium glandulosum roots. The traditional Chinese medicine composition disclosed by the invention can intervene in liver injury through a multi-target and multi-channel synergistic effect, and has good anti-hepatitis and anti-hepatic fibrosis effects.

The invention relates to a watermelon seed vigor grade classification method based on terahertz transmission and reflection imaging data fusion. The method comprises the following steps: firstly, performing artificial accelerated aging treatment on watermelon seeds under high-temperature and high-humidity conditions to obtain samples with different activity grades; then, a terahertz time-domain spectral imaging system is used for collecting transmission and reflection signals respectively, the transmission signals are subjected to color threshold segmentation to extract an embryo region, and the reflection signals are subjected to Canny edge detection and contour screening to extract a surface region; and after the obtained signal is subjected to smooth filtering and deconvolution processing, first-order derivative difference enhancement is further introduced, and a fusion spectrum is constructed by adopting a weighted fusion strategy. The fusion spectrum is subjected to weight distribution through a frequency attention mechanism and then input to a feature perception convolutional neural network, the network comprises two layers of one-dimensional convolution and nonlinear activation functions and is provided with high, medium and low activity branch classification paths, and finally a classification result of seed activity levels is output.

The utility model relates to the technical field of watermelon seed kernel production and processing, in particular to a watermelon seed kernel cleaning and drying all-in-one machine which comprises a processing shell and a feeding opening formed in the top of the processing shell, and further comprises a cleaning assembly arranged on one side of the processing shell, a heating assembly is installed on the top of the processing shell, and an inner cavity of the processing shell is rotationally connected with a spraying pipe. A material guide plate is mounted in an inner cavity of the processing shell; under the cooperation of the stepping motor and the vibration mechanism, the moving rod can drive watermelon seed kernel materials to vibrate through the material guide plate, the situation that the materials are distributed unevenly due to accumulation of the cleaned materials is effectively reduced, and therefore the drying effect of the spray head on the materials is improved; and meanwhile, under cooperation of a rotating rod, a bevel gear set and a swing mechanism, a third spur gear can drive a spray pipe to swing in an inner cavity of the machining shell in a reciprocating mode, the hot air spraying area of the spray pipe is increased, and therefore the drying effect of the spray pipe is improved.

The present invention relates to an herbal composition and method for a treatment and management of at least one of hypertension, hyperlipidemia, diabetes mellitus, and associated cardiovascular risk factors. The composition comprises standardized plant extracts derived from Rauvolfia serpentina, Hippophae rhamnoides seed, Citrullus lanatus seed, and Phyllanthus emblica fruit, in approximate weight proportions ranging from 20-40% for Rauvolfia serpentina, 20-40% for Hippophae rhamnoides, 10-30% for Citrullus lanatus, and 10-30% for Phyllanthus emblica. The invention also provides a method of preparing the composition involving harvesting, drying, grinding, extracting with selected solvents, concentrating, optionally fractionating, and formulating into oral dosage forms such as one of capsules, tablets, and powder sachets. The invention further includes methods for administering the composition to a subject in need thereof, optionally as an adjunct to conventional therapy.

The utility model provides watermelon seed cleaning and sorting equipment, and relates to the technical field of seed cleaning, the watermelon seed cleaning and sorting equipment comprises a placing table, a soaking tank is fixed on the surface of the placing table in a penetrating manner, the lower end of the soaking tank is provided with a liquid discharge pipe orifice, and the surface of the liquid discharge pipe orifice is fixedly provided with a valve; a first electric telescopic rod is fixed to the outer surface of the soaking tank, and after watermelon seeds in the sorting frame are soaked and cleaned in saline water in the soaking tank, the first electric telescopic rod extends to move the sorting frame upwards, so that the watermelon seeds are not soaked in the saline water; and then two second electric telescopic rods are controlled to stretch and retract back and forth to drive a connecting arm and a sorting frame to shake left and right, the wizened and fine inferior seeds are screened out and fall off from the sorting frame, and therefore the high-quality seeds with full particles are sorted out from the sorting frame, manual screening is not needed, and simplicity and rapidness are achieved.

The invention relates to the technical field of agricultural application, in particular to a method for promoting watermelon seed germination under a high-temperature stress condition. The invention discloses a method for promoting germination of watermelon seeds under a high-temperature stress condition. The method comprises the following steps: (1) soaking disinfected watermelon seeds in a nanoscale magnesium oxide solution; (2) wrapping the soaked watermelon seeds with wet cloth, and then accelerating germination. The invention provides an effective technical means for solving the problems of low seed germination rate, uneven seedling emergence and the like in watermelon seedling culture in the high-temperature area, is beneficial to guaranteeing the stability and economic benefit of watermelon production, and has positive significance for promoting agricultural sustainable development.

The invention belongs to the technical field of vegetable cultivation, and particularly relates to a sulfur-based fertilization method for promoting watermelon growth and improving quality and application. The fertilization method comprises the following steps: mixing peat, vermiculite and perlite according to a volume ratio of 3: 1: 1 to prepare a seedling culture substrate, adding sulfur powder, uniformly mixing, adjusting the water content to 55-65%, using the substrate as a base fertilizer, standing for 10 days, sowing watermelon seeds after germination acceleration, and carrying out seedling culture; when the watermelon seedlings grow to a one-leaf and one-core period, topdressing is started; the topdressing is a normal-sulfur Hoagland nutrient solution or a low-sulfur Hoagland nutrient solution, the topdressing is applied once every two days, the dosage of each time is 50 mL / plant, and the topdressing is continuously applied to the early stage of watermelon vine growing; and performing conventional pruning, pollination and fruit thinning management in and after the watermelon vine growing period until fruits are mature. The fertilizing method can promote plant growth, enhance oxidation resistance, and significantly improve watermelon fruit quality.

The present invention provides novel watermelon line 54WA028 and plant parts, seed, and tissue culture therefrom. The invention also provides methods for producing a watermelon plant by crossing the watermelon plants of the invention with themselves or another watermelon plant. The invention also provides watermelon plants produced from such a crossing as well as plant parts, seed, and tissue culture therefrom. Further provided are methods of producing triploid watermelon seed and plants and seedless watermelon fruit produced therefrom as well as the triploid watermelon seed and plants and the seedless fruits produced by such methods.

This application relates to the field of cultivation box technology and discloses an adjustable cultivation box for ecological watermelon seeds, including a cultivation box body, a mounting frame, and a water tank. A sealing door is movably installed at the front end of the cultivation box body, and the mounting frame is slidably installed on the inner side of the cultivation box body. A cultivation trough is installed at the upper limit of the mounting frame, and a water tank is fixedly installed on one side of the cultivation box body. This application allows water to be easily filled into the water tank through the water inlet, and the liquid inside the water tank can be easily extracted by the water pump and discharged through the nozzle. At the same time, the rotation of the motor output end can cause the lead screw to rotate synchronously, so that the lead screw and the moving plate are threadedly engaged, so that the mounting plate can move along the inside of the cultivation box body during the movement of the moving plate. The watermelon seeds on the cultivation trough can be sprayed through the nozzle.

The invention is applicable to the technical field of agricultural disease prevention and control, and provides a method for efficiently and nondestructively treating seeds carrying bacterial fruit blotch pathogenic bacteria and application, the method comprises the following steps: S1, treating the seeds carrying diseases by using low-temperature plasma; s2, performing molecular biological detection on the treated seeds; s3, sowing the treated seeds into a seedling raising tray for seedling raising; a dielectric barrier discharge (DBD) device is adopted in the low-temperature plasma treatment in the step S1. The low-temperature plasma treatment method has an obvious killing effect on watermelon acidophilic bacteria inside and outside the seeds, the seed treatment efficiency is improved, the purpose of preventing and controlling the melon bacterial fruit blotch is achieved, the risk of the diseases is effectively avoided, and huge economic losses caused by the fruit blotch of farmers and enterprises are reduced; the seed vigor can be enhanced, the bud vigor and bud rate of the watermelon seeds can be remarkably improved, the germination and growth period of the seeds can be shortened, the seedling emergence uniformity of the seeds can be improved, and the seedling commodity can be improved.

The utility model relates to the technical field of watermelon seed screening, and discloses a seed screening device for watermelon planting, which solves the problems proposed in the background technology, and comprises a screening mechanism and a water treatment mechanism, the screening mechanism comprises a feed inlet, a water tank is arranged at the bottom of the feed inlet, a rotating motor is arranged at the bottom of the water tank, and the water treatment mechanism is arranged at the bottom of the water tank. The output end of the rotating motor penetrates through the bottom of the water tank to be connected with a screw rod, the screw rod is arranged below the feeding port, a nozzle is arranged on the side wall of the side, close to the feeding port, in the water tank, a fishing port is formed in the water tank, a vertical plate is arranged on the water tank, a sliding rail is arranged on the vertical plate, a sliding block is arranged on the vertical plate in a sliding mode, and the sliding block is connected with the screw rod through a screw rod. The watermelon seed screening device has the advantages that the density difference of watermelon seeds can be utilized, full high-quality seeds and empty and shriveled and dysplastic seeds can be accurately separated out, the seed quality is guaranteed, and the screening efficiency is improved.

The utility model discloses watermelon seed heating stir-frying equipment, and relates to the technical field of watermelon seed processing, the watermelon seed heating stir-frying equipment comprises a bottom plate, the middle ends of the left and right sides of the top of the bottom plate are fixedly connected with vertical plates, the upper ends of the inner sides of the vertical plates are fixedly connected with stir-frying heating cylinders through bearings, and the tops of the outer sides of the vertical plates are fixedly provided with motors through bolts; the output end of the motor is fixedly connected with a transmission rod, the middle end of the surface of the transmission rod is fixedly connected with a rotating cylinder, through the relation among the motor, the transmission rod, the rotating cylinder and the stirring blades, watermelon seeds can be stirred, the watermelon seeds can be evenly heated in the stir-frying process, meanwhile, the stirring efficiency is improved, and the watermelon seeds can be evenly heated. And the watermelon seeds can make contact with seasoning, through the relation among a second belt wheel, a belt, a first belt wheel, a rotating rod, a gear, teeth and a fluted disc, the watermelon seeds can shake again, and the heating efficiency of the watermelon seeds can be further improved.

1. Name of the designed product: melon seed machine (dinosaur). 2. Use of the designed product: melon seed machine. 3. Design points of the designed product: in shape. 4. Picture or photo that best shows the design points: perspective view 1.

The present invention discloses the use of Pseudomonas in the preparation of a biological agent for preventing and treating watermelon wilt, belonging to the field of biotechnology. By applying Pseudomonas and its fermentation products during watermelon cultivation, the present invention effectively inhibits the progression of Fusarium-infected watermelons and achieves a relatively ideal control effect. Furthermore, the fermentation products of Pseudomonas can also promote the germination of watermelon seeds.

The invention relates to the technical field of application of microorganisms, in particular to a phosphate solubilizing bacterium BPC41 and application thereof. The salt-tolerant bacteria are separated from the saline-alkali soil by adopting a soil dilution coating method and a plate streaking method, and the salt-tolerant bacteria BPC41 are separated and identified from the Tangshan saline-alkali soil by re-screening based on the phosphate solubilizing capacity of the salt-tolerant bacteria. A comprehensive characteristic determination result shows that the strain has excellent saline-alkali tolerance and a multi-element growth-promoting function, specifically, the strain can efficiently decompose organic phosphorus, dissolve inorganic phosphorus and fix nitrogen, and meanwhile has the growth-promoting characteristics of secreting indoleacetic acid and forming a biological membrane. A germination test result shows that the BPC41 strain not only can promote germination of red bean seeds, lettuce seeds, cucumber seeds, wheat seeds and watermelon seeds, but also can promote growth of soybean seed buds under salt stress and relieve salt stress. Pot experiment results show that the plant growth can be promoted under the saline-alkali stress condition.

The utility model relates to the technical field of watermelon seeds, and discloses a watermelon seed germination accelerating box which comprises a box body, a placement frame is fixedly connected to the inner wall of the box body, germination accelerating discs are slidably connected to the outer wall of the placement frame in a linear array mode, and a plurality of mounting frames are fixedly connected to the position, above the multiple germination accelerating discs, of the box body; the box body is provided with a connecting pipeline through a plurality of mounting frames, the outer wall of the connecting pipeline is provided with a plurality of spraying heads corresponding to the germination accelerating discs, the outer walls of the two sides of the box body are fixedly provided with temperature sensors, the outer wall of the bottom of the box body is fixedly provided with a heater, and the outer wall of the heater is provided with a plurality of air outlets; the watermelon seed germination accelerating box has the advantages that the constant temperature of the watermelon seed germination accelerating box can be kept, the humidity can be controlled, the conditions of mildewing of seeds and the like can be avoided, the operation of workers is facilitated, the germination rate and the germination speed of watermelon seeds are greatly improved, and the subsequent unified seedling culture management is also facilitated.

The utility model relates to the technical field of watermelon seed kernel production and processing equipment, in particular to a raw material impurity removing device for watermelon seed kernel production and processing, which comprises a bottom plate and further comprises a conveying part which is fixed at the top of the bottom plate and used for conveying watermelon seed kernels, a fixing rod is fixed at the top of the bottom plate, and a flow guide frame is fixed at the upper end of the fixing rod. A plurality of groups of small holes facilitating air circulation are formed in the inner wall of the flow guide frame, a fixing plate is fixed to the outer side of the fixing rod, the flow guide frame can be conveniently fixed by arranging the fixing rod, the fixing plate can be conveniently fixed, the blowing fan can be conveniently installed by arranging the fixing plate, and the blowing fan can be conveniently installed by arranging the fixing plate. Impurities in watermelon seed kernel raw materials flowing down from the flow guide frame can be conveniently blown into the connecting frame, and the impurities can be conveniently stored through cooperative use of the connecting frame and the storage frame.

The invention discloses a method for quantitatively detecting cucumber green mottle mosaic virus (CGMMV) infectious viruses in watermelon seeds based on propionium bromide azide (PMA) pretreatment and reverse transcription quantitative polymerase chain reaction (RT-qPCR). The method comprises the following steps: S1, preparing a seed suspension containing virus particles; s2, grouping the suspensions, adding PMA with the final concentration of 150-170 [mu] M into a PMA treatment group, incubating for 20-40 min in a dark place, then carrying out blue light (460-490 nm) irradiation to enable PMA to selectively inhibit non-infectious virus nucleic acid amplification, and not adding PMA into a control group or carrying out inactivation treatment on the control group; s3, extracting nucleic acid, and carrying out RT-qPCR (real-time quantitative polymerase chain reaction) on the nucleic acid by adopting a specific primer pair CG-2439F / CG-2625R to obtain a Cq value or a nucleic acid amount; and S4, comparing the results of the PMA treatment group and the control group to determine the infectious CGMMV content or the live virus proportion. The method can reduce inactive virus interference, is suitable for seed quarantine, virus risk assessment and disinfection effect evaluation, and can provide a matched detection kit.