9 results about "RNA extraction" patented technology

The present application relates to the technical field of RNA extraction, and particularly relates to a pretreatment reagent, a method and a kit for RNA purification of whole blood samples. By introducing hydrophobic DESs as the core pretreatment component, the DESs are mixed with the whole blood sample in advance, the DESs form an independent phase layer after centrifugation by using the unique physicochemical properties, and the liposoluble drug components, inflammatory proteins and pigments are selectively extracted to the hydrophobic DESs phase and the RNA is preliminarily purified; the upper water phase of the pretreatment and purification is taken and added into a lysis solution containing a chaotropic salt to fully release the nucleic acid, and DNase I is introduced for digestion. The present application combines the pretreatment of hydrophobic DESs and the digestion of DNase I, and is used in combination with PVP (targeting adsorption of residual pigments and phenolic compounds), reduces the impurities of the lysis system through the front-end pretreatment, creates a low-interference and high-efficiency purification environment for the specific binding of RNA by the magnetic beads, and forms a synergistic removal network for the interference of complex samples.

The application discloses a DNA / RNA reagent kit based on a magnetic bead method and free of enzyme digestion, and belongs to the technical field of nucleic acid extraction and purification. The reagent kit comprises a lysis solution AL, protease K, a specific binding buffer CLB, amino magnetic bead suspension, silicon hydroxyl magnetic bead suspension and other components. The application solves the problems of nucleic acid degradation, incomplete removal of cross contamination and complicated process caused by enzyme digestion in the prior art, is suitable for automatic high-throughput processing, and has a wide application prospect in scientific research and clinical application.

The present application relates to a primer combination for lung cancer detection and its application in lung cancer diagnosis, in particular, a technology for specifically detecting CIZ1b splice body RNA through real-time fluorescent quantitative PCR technology. The method combines blocker sequence to inhibit non-specific amplification of CIZ1a splice body by designing specific primers and probes, and ensures high-specificity detection of CIZ1b splice body RNA. The detection samples include lung cancer tissues and plasma, and cDNA is generated through reverse transcription after RNA extraction of the samples, and real-time fluorescent quantitative analysis is performed. Meanwhile, the method introduces an internal reference gene B2M for standardization correction, and improves the sensitivity and accuracy of the detection. The present application provides a non-invasive and reliable early lung cancer diagnosis tool, which has good clinical application potential.

This invention belongs to the field of biological detection technology, and particularly relates to a reproductive tract HPV-mRNA-E6 / E7 detection system and method based on nanopore sequencing. The system includes a sample pretreatment module, an RNA extraction and purification module, a reverse transcription module, a nanopore sequencing module, and a bioinformatics analysis module. The method includes the following steps: (1) reproductive tract sample collection and pretreatment; (2) extraction and purification of HPV-mRNA in the sample; (3) reverse transcription of the purified HPV-mRNA into cDNA; (4) nanopore sequencing of the cDNA to obtain raw sequencing data; (5) filtering, comparing, and annotating the sequencing data through the bioinformatics analysis module to achieve HPV type identification and quantitative analysis of E6 / E7 gene expression levels. This invention utilizes the advantages of nanopore sequencing, such as long read length and real-time detection, combined with the specificity of E6 / E7 mRNA as a biomarker of active infection. It can directly detect latent and active HPV infection, improve detection sensitivity, accurately distinguish infection types, and shorten the detection cycle. It enables rapid, accurate, and high-throughput detection of active HPV infection in the genital tract, providing reliable technical support for early screening, disease monitoring, and prognostic assessment of HPV-related diseases such as cervical cancer.

The application discloses an inbreeding analysis method and system for high-throughput sequencing and electronic equipment, and relates to the technical field of animal molecular detection, and comprises the following steps: obtaining a sample set of adult inbred boars and a sample set of Dianan boars, performing RNA extraction, and obtaining a sample RNA set of adult inbred boars and a sample RNA set of Dianan boars; performing sample quality comprehensive authentication on the sample RNA set of adult inbred boars and the sample RNA set of Dianan boars according to a preset multi-dimensional sample RNA quality evaluation weight set, and obtaining an authentication result; when the authentication result is authentication passed, performing high-throughput sequencing based on the sample RNA set of adult inbred boars and the sample RNA set of Dianan boars, and performing inbreeding analysis according to a sequencing result. The application solves the technical problem of poor sequencing data reliability in pig inbreeding analysis in the prior art, and achieves the technical effect of improving the accuracy of high-throughput sequencing and the reliability of inbreeding analysis results.

The application provides a malt PYF performance prediction method based on barley gene expression in a malting process and an application thereof, belongs to the field of biology, and can solve technical problems of traditional malt PYF detection methods, such as complicated operation steps, long time consumption, and narrow application range. The prediction method specifically comprises the following steps: candidate gene screening, tracking gene screening, reverse transcription reaction, multiple PCR reaction primer design, RNA extraction, reverse transcription cDNA template preparation, multiple PCR reaction, PCR amplification product electrophoresis and expression analysis, and malt PYF performance prediction. By comparing the gene expression differences of standard malt and PYF malt in the malting process, tracking genes are selected in the large production process, and then by comparing the expression amounts of the standard malt and the to-be-detected malt and combining the evaluation standard, the malt PYF performance can be rapidly and accurately analyzed. The application can be applied to the field of malt PYF performance prediction.

This invention discloses a stimulus-responsive polymer magnetic bead, its preparation method, and a method for targeted extraction of ribonucleic acid (RNA), belonging to the fields of biomedical nanomaterials and molecular diagnostics. The polymer magnetic bead comprises a magnetic carrier and a stimulus-responsive polymer grafted onto its surface. This polymer contains thermosensitive N-isopropylacrylamide segments and functional monomer segments with phosphate group affinity. The preparation method includes surface bromination of the magnetic carrier, followed by grafting the copolymer via atom transfer radical polymerization. In RNA extraction applications, these magnetic beads utilize the stimulus-responsive properties of the polymer chains to achieve dynamic adsorption and static elution, enabling direct and efficient capture of RNA from tissue lysate. This invention significantly improves the concentration, purity, and integrity of extracted RNA, while simplifying the operation steps, shortening the extraction time, and greatly reducing the use of toxic reagents, offering outstanding advantages such as low cost, high efficiency, and environmental friendliness.

The present application relates to the technical field of pneumonia, and discloses a treatment method for pneumonia, S1: preliminary evaluation and diagnosis: S1.1: check symptoms (such as cough, fever, dyspnea, etc.); S1.2: physical examination, auscultation of the chest; S1.3: chest X-ray examination or CT scan to observe the lung image; S2: determine the specific pathogen through genome sequencing: S2.1: sample collection: first collect clinical samples from the patient, such as blood, sputum or bronchoalveolar lavage fluid; S2.2: DNA / RNA extraction: extract the genetic material of microorganisms from the sample, whether it is DNA or RNA, depending on the type of pathogen. The treatment method for pneumonia can accurately determine the type of pathogen causing pneumonia and its drug resistance, which helps to implement a more personalized and precise treatment plan. This not only can improve the success rate of treatment and reduce unnecessary broad-spectrum antibiotic use, but also helps to slow down the growth rate of antibiotic resistance.