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291 results about "Variant strain" patented technology
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Porcine pseudorabies virus (PRV) variant PRV-ZJ01 and application thereof
ActiveCN103627678AImprove securityImproving immunogenicityMicroorganism based processesAntiviralsRabiesEngineering
Owner:NANJING AGRICULTURAL UNIVERSITY
gE- and gI-deleted porcine pseudorabies virus variant strain and use thereof
The invention relates to the technical field of porcine pseudorabies viruses and especially relates to a gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G and a use thereof. The gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G has the accession number of CGMCC No.7957. The invention discloses the use of the gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G in vaccine preparation. After the New Zealand big white rabbit is inoculated with the 106.0TCID50 recombinant viruses, clinical symptoms such as pruritus are not caused. An oil-in-water inactivated vaccine prepared from the gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G is injected into a piglet and after four weeks, the BELISA antibody is produced but the gE antibody does not exist, and the immunization protection efficiency is 100%. After immunization on sows, the piglets produced by the sows get immunization protection and the efficiency of PRV variant virus and traditional virus immunization protection is 100%. It is proved that the ZJ011G recombinant virus has good immunogenicity and can be used for vaccine preparation.
Owner:JIANGSU NANNONG HI TECH
Recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus genetic engineering strain and application
InactiveCN101457215AAvoid infectionPrevention of swine pseudorabiesViral antigen ingredientsMicroorganism based processesAnimal virusAntigen
The invention belongs to the animal virus genetic engineering technique field, especially to a recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus gene engineering strain construction, a vaccine preparation and applications. The recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus gene engineering strain E001 lacks the pseudorabies virus main virulence gene TK, glycoprotein genes gG, gE and gI, does not express the functional glycoprotein gG and gE / gI of the pseudorabies virus; and simultaneously expresses a GP5m / M protein antigen of the porcine propagate and breath complex virus classical strain and the porcine propagate and breath complex virus internal variant GP5 protein and porcine circovirus ORF2. The strain can stimulate the porcine to produce protective immunity reaction for resisting pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus, effectively prevent the infection of the pseudorabies virus, the porcine propagate and breath complex virus and porcine circovirus. The invention also discloses a preparation and application of a tervalence genetic engineering vaccine.
Owner:HUAZHONG AGRI UNIV
Fusion protein of SARS-CoV-2, and vaccine composition of fusion protein
InactiveCN111662389AInhibition of replicationRefrain from rebroadcastingSsRNA viruses positive-senseAntibody mimetics/scaffoldsSpecific immunityVariant strain
The invention belongs to the technical field of biology, and particularly relates to a fusion protein of SARS-CoV-2, and a vaccine composition of the fusion protein. The fusion protein and the prepared vaccine composition containing the fusion protein overcome the defects of poor immunogenicity and the like of subunit vaccines, can induce the specific immune response aiming at SARS-CoV-2, and achieve the purposes of inhibiting the replication of SARS-CoV-2, inhibiting the spread of SARS-CoV-2 or preventing the settlement of SARS-CoV-2 common strains and variant strains in SARS-CoV-2 host bodies. The fusion protein and the vaccine composition containing the fusion protein can effectively prevent and / or treat novel coronavirus pneumonia (Corona Virus Disease 2019, COVID-19). The fusion protein can be recombined and expressed in large quantities by the aid of the genetic engineering technology, the consumed time is short, and the large-scale production can be facilitated.
Owner:GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
O type foot-and-mouth disease virus variant as well as coding gene and application thereof
The invention discloses an O type foot-and-mouth disease virus variant as well as a coding gene and application thereof. In the invention, an O type foot-and-mouth disease virus pan-Asia strain O / YS / CHA / 05 is firstly separated out, the nucleotide sequence of the O type foot-and-mouth disease virus pan-Asia strain O / YS / CHA / 05 SEQ ID NO: 1, and the amino acid sequence is SEQ ID NO: 2. In comparison with a VP1 amino acid sequence, the virus strain has 7 variable sites, five of which are centralized in a G-H ring. Mutation of the sites ensures that the virus variant has the capability of escaping from host immunity so as to have the superiority for becoming a popular virus strain. Therefore, the variant can be employed to prepare an inactivated vaccine for prevention and treatment of the variant and relevant strains, dominant antigen epitope of the variant can be employed to prepare a synthetic peptide vaccine, and the variant can be employed to develop novel O type foot-and-mouth disease virus vaccines such as VLP vaccine and the like. Therefore, the invention has important value in controlling the popularity of O / YS / CHA / 05 and relevant variable strains.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Carboxylic Acid Producing Member of the Pasteurellaceae
The present invention relates to a novel bacterial strain designated DD1, which has the ability to produce organic acids, in particular succinic acid (SA), which was originally isolated from bovine rumen, and is capable of utilizing glycerol as a carbon source; and variant strains derived there from retaining said capability; as well as to methods of producing organic acids, in particular succinic acid by making use of said microorganism.
Owner:BASF AG
Digital PCR (Polymerase Chain Reaction) detection kit for novel coronavirus and variants thereof
ActiveCN113073150AMicrobiological testing/measurementAgainst vector-borne diseasesWild typeVariant strain
The invention belongs to the field of molecular detection, and particularly relates to a digital PCR (polymerase chain reaction) detection kit for the novel coronavirus and variants thereof. Firstly, the invention discloses a primer probe composition for simultaneously detecting the novel coronavirus and the variants thereof, wherein the novel coronavirus comprises a wild type, a B.1.1.7 variant, a B.1.351 variant and a P.1 variant; the primer probe composition is designed according to the N gene, the ORF1ab gene and the mutation types of HV69-70del, K417N, K417T and N501Y of the S gene. The invention develops a novel multiple digital PCR system and a kit for detecting the novel coronavirus and the variants thereof by using the PCR system. The multiple digital PCR system solves the problems that different novel coronavirus variants in the prior art need to be separately detected for several times, and the process is tedious.
Owner:领航医学科技(深圳)有限公司
New grey white variant strain with red pleurotus
The invention relates to a new strain of edible fungus, belonging to the technical field of edible fungus development and utilization. The new grey variant strain of edible fungus with red pleurotus: Pleurotus djamor (Rumph.: Fr.)Boedijn, is the new strain of edible fungus selectively bred through single spore cross breeding and has the number No.1933 in CGMCC (China General Microbiological Culture Collection Center). The strain is characterized in that the color of sporophore is stable grey white, the whole sporophore is pure and transparent, the cellulose content is 5-12%, and the strain has favorable mouth feel and is used for producing the edible fungus in the high temperature season. Mycelium grows very fast and has strong ability in fighting bacteria and also has developed aerial hyphae. The hypha has clamp connection and the growth temperature of 3-45 DEG C; the temperature of sporophore differentiation is 18-35 DEG C, and the new grey variant strain is typical high temperature edible fungus. Straws, saw dust, bran, and the like are selected as cultivation materials. The edible fungus is cultivated in March to November. Cultivation materials produced in scale do not need high pressure or normal pressure sterilization, and the sporophore output is 15-25%. The strain is newly selected and bred edible fungus and has application value in the edible fungus field and ornamental fungus field.
Owner:SOUTHWEAT UNIV OF SCI & TECH
Porcine reproductive and respiratory syndrome virus RT-LAMP detection kit and detection method thereof
ActiveCN101575650AEfficient and specific amplificationThe detection process is fastMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementRNA extractionHighly pathogenic
The invention relates to a porcine reproductive and respiratory syndrome virus RT-LAMP detection kit and a detection method thereof. Primers required by PRSSV RT-LAMP reaction system are designed according to the sequence of porcine reproductive and respiratory syndrome virus (PRSSV) published by GenBank; PRSSV virus RNA is extracted with the virus RNA extraction reagent (LBBII-RNA) designed and prepared by the inventor, the PRSSV RT-LAMP reaction system established in the invention is utilized for detection, and color developing agent is added after the reaction to judge the result; the result shows that the PRSSV virus RNA obtains efficient specific amplification after the reaction is conducted for 45 minutes at the temperature of 63 DEG C; and then, quick detection of porcine reproductive and respiratory syndrome virus American classical strain and NSP2 variant strain (highly pathogenic porcine reproductive and respiratory syndrome virus strain) is conducted by SpuI enzyme cutting. Compared with the prior art, the invention has quick detection, high sensitivity, low reaction cost, convenient and fast operation, which is capable of differentiating American classical strain and NSP2 variant strain (highly pathogenic porcine reproductive and respiratory syndrome virus strain) and meets the requirement of multi-level detection.
Owner:CHINA INST OF VETERINARY DRUG CONTROL
Virus-like particle recombinant protein of virus variation strain VP2 gene of infectious bursal disease
InactiveCN101624421ANon-infectiousImproving immunogenicityViral antigen ingredientsVirus peptidesVp2 geneVirus-like particle
The invention relates to virus-like particle recombinant protein of a virus variation strain VP2 gene of an infectious bursal disease, belonging to the field of biologic pharmacy. The IBDV variation strain (AH1) VP2 gene is cloned, converted and transfected to obtain a recombinant baculovirus vBac-VP2; an infected Sf9 insect cell has specific fluorescence, and the antigen valence of the infected Sf9 insect cell is above 1.6*10<3>; the molecular weight of a recombinant VP2 protein is 53kDa, and the recombinant VP2 protein is in the state of virus-like particles; an indirect ELISA detection method established for an envelope antigen by the purified recombinant VP2 protein has good specificity and sensibility; an immune chicken can resist IBDV virulent attack, and the protection ratio achieves 100 percent. The novel virus-like particle recombinant VP2 protein prepared by the IBDV variation strain VP2 gene has high pertinence on the immune prevention of a current prevalent IBDV virulent strain and good practical value and popularization prospects.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Establishment of MDCK cell line stably expressing beta-galactoside alpha-2, 3-sialytransferase I(ST3GAL I)
The invention provides an MDCK-ST3GAL I cell line, concretely an MDCK cell line stably expressing beta-galactoside alpha-2, 3-sialytransferase I(ST3GAL I) with the preservation number of CGMCC No. 2620; the invention further provides a method for establishing the MDCK-ST3GAL I cell line and a method for improving the growth titre of avian influenza virus and the forming capability of plaque. The invention further provides an application of the MDCK-ST3GAL I cell line in researching avian influenza virus 2, 3 linked receptor tendency; and the invention is used for applications in monitoring the avian influenza virus receptor associative property variant strain, screening medicines resisting avian influenza virus, measuring the neutralizing antibody and mass producing cell culture vaccine.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Porcine pseudorabies virus gene deletion attenuated vaccine strain for passage via low temperature of cell and attenuation via drug screening and application thereof
ActiveCN106282128AImprove securityImprove protection efficiencyViral antigen ingredientsMicroorganism based processesLarge fragmentVariant strain
The invention discloses a porcine pseudorabies virus gene deletion attenuated vaccine strain for passage via low temperature of a cell and attenuation via drug screening and an application thereof. The attenuated vaccine strain is prepared through the following steps: based on a porcine pseudorabies virus variant strain (named as strain HeN1, of which the microbial preservation serial No. is CGMCC No. 6656), firstly, carrying out low-temperature passage and screening on a Vero cell to obtain large fragments of deleted viruses including gI, gE, Us9, Us2 and part of inverted repeated sequence which exist in zone US through, and then making the TK gene thereof partially deleted through drug screening. The gene-deleted attenuated vaccine strain is named as strain PRV TP, of which the microbial preservation serial No. is CGMCC No. 12300. A live vaccine or an inactivated vaccine (a single vaccine or combined vaccine) can be prepared from the attenuated vaccine strain disclosed by the invention, and can prevent porcine pseudorabies effectively, and a reagent for diagnosing or treating porcine pseudorabies can be prepared from the attenuated vaccine strain too. According to the porcine pseudorabies virus gene deletion attenuated vaccine strain for passage via low temperature of a cell and attenuation via drug screening and the application thereof, the porcine pseudorabies attenuated vaccine strain PRV TP has the advantages of good safety, efficient protection, convenient differential diagnosis and the like.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
A-type antigen polypeptide, fusion antigen polypeptide and vaccine of foot and mouth disease virus
ActiveCN105418738AImproving immunogenicityBroad spectrum immunitySsRNA viruses positive-senseAntibody mimetics/scaffoldsImmunogenicityBroad spectrum
The present invention discloses an A-type antigen polypeptide, a fusion antigen polypeptide and a vaccine of a foot and mouth disease virus, in particular relating to an A-type antigen polypeptide and a fusion antigen polypeptide of the foot and mouth disease virus, and a foot and mouth disease virus vaccine containing the antigen polypeptide and / or a fusion antigen polypeptide. The present invention also provides preparation methods of the antigenic polypeptide, the fusion antigen polypeptide and the vaccine. The present invention further provides applications of the antigen polypeptide, the fusion antigen polypeptide and the vaccine in preventing and controlling of the foot and mouth disease virus infection. The A-type antigen polypeptide, the fusion antigen polypeptide and the vaccine of the foot and mouth disease virus disclosed by the present invention have a broad spectrum immunogenicity, and may produce good immunogenicity on different foot and mouth disease viruses and variants thereof.
Owner:SHANGHAI SHEN LIAN BIOMEDICAL CORP +1
Novel coronal virus strain and medicinal use thereof
The present invention relates to a new strain of coronavirus and its application. Said invention utilizes the throat-swabbed cell colture of SARS patient to separate out a new type coronavirus, called by name of SARS coronavirus Fudan I strain. By infecting Vero E 6 cell and patient's serum the indirect FA display is positive, after the influenza virus A and B, RS virus and pathogens of pneumococcus, etc. are excluded, the tests show that said separated coronavirus is a variant strain of new coronavirus. Said strain can be used for providing basis for preparing SARS diagnosis reagent and researchnig and producing medicine for resisting and curing SARS disease.
Owner:FUDAN UNIV
Foot and mouth disease virus antigen polypeptide, fusion antigen polypeptide and vaccine
InactiveCN102180952AImproving immunogenicityImprove securityVirus peptidesAntiviralsVariant strainImmunogenicity
The invention provides a foot and mouth disease virus antigen polypeptide, a foot and mouth disease virus fusion antigen polypeptide and a foot and mouth disease virus vaccine containing the antigen polypeptide and / or the fusion antigen polypeptide. The invention further provides application of the antigen polypeptide, the fusion antigen polypeptide and the vaccine in prevention and control of foot and mouth disease virus infection. The foot and mouth disease virus antigen polypeptide, the fusion antigen polypeptide and the vaccine have broad-spectrum immunogenicity, and can generate good immunogenicity on different foot and mouth disease viruses and variant strains thereof.
Owner:SHANGHAI SHEN LIAN BIOMEDICAL CORP
Composition, kit and method for detecting SARS-CoV-2 variant and application
ActiveCN113981152AMicrobiological testing/measurementAgainst vector-borne diseasesVariant strainBiomedical engineering
The invention belongs to the field of molecular biological detection, and relates to the detection of a SARS-CoV-2 Omicron variant. The invention provides a kit containing the composition, application of the composition and a method for detecting and typing the SARS-CoV-2 variant. By detecting four different characteristic function variation sites on the S gene of the SARS-CoV-2 variant, the Omicron variant is subjected to typing, so that the typing detection of the SARS-CoV-2 virus and the Omicron variant is simultaneously realized in a single-tube reaction system. The composition disclosed by the invention is combined with a fluorescent probe melting curve method, and is low in cost and high in flux. Moreover, the operation is simple and convenient, and the result reading process can be judged through a melting peak Tm value. The whole detection process is carried out under a single-tube closed condition, so that false positive and environmental pollution caused by crossing of samples are avoided.
Owner:SHENZHEN UNI MEDICA TECH
Primer, probe composition and kit for detecting novel coronavirus and variant thereof
ActiveCN113817868ALow costVariant information richMicrobiological testing/measurementMicroorganism based processesReference genesGenes mutation
The invention discloses a primer, a probe composition and a kit for detecting a novel coronavirus and a variant thereof. The primer and the probe composition comprise specific primers and probes aiming at an ORF1ab gene, an N gene and a reference gene of SARS-CoV-2, and further comprises specific primers and probes aiming at one or more mutation sites of 69-70Del, L452R, E484K, E484Q and N501Y mutation sites of an S gene of the SARS-CoV-2, 106 / 107 / 108del mutation sites of an NSP6 gene, P71L mutation sites of an E gene, V82A mutation sites of an ORF7A gene and S253P mutation sites of an ORF3A gene. The primer, the probe composition and the kit disclosed by the invention can be used for detecting mutation sites while detecting the novel coronavirus, and can be used for distinguishing one or more of a B.1.351 variant, a B.1. 1.7 variant, a B.1.617 variant or a P.1 variant of the novel coronavirus; and the kit is a novel coronavirus and variant detection reagent which is rapid, low in cost and rich in variation information, and provides powerful help for global epidemic situation prevention and control and epidemic disease research.
Owner:成都峰际生物技术有限公司
TK, ge and gT gene deletion strain of porcine pseudorabies virus variant and application of TK, ge and gT gene deletion strain
InactiveCN106834236AFight infectionNo reverse mutationVirus peptidesMicroorganism based processesCytopathic effectVariant strain
The invention discloses a TK, ge and gT gene deletion strain of a porcine pseudorabies virus (PRV) variant ZJ01 and application of the TK / ge / gT gene deletion strain. The strain is the TK / ge / gT gene deletion strain ZJ01delta TK / gE / gI (ZJ01R in short) of the PRV ZJ01 strain. According to the in-vitro passage test results, gE / gI gene deletion strains ZJ01delta gE / gI of a recombinant virus ZJ01R and the PRV ZJ01 strain are consistent with cytopathic effect generated by a parental virus ZJ01, and virus titer is similar with the ZJ01 strain; a target gene deletion region of the recombinant virus ZJ01R continuously and stably exists without resilience mutation; according to the pig immunity and attacking protection test, after the recombinant virus ZJ01R is immune, a pig is free of clinical symptoms and pathological changes; after being immunized by 21 days, the pig can generate a PRV neutralizing antibody with relatively high level, and can resist challenging infection of a fatal PRV variant strain ZJ01.
Owner:NANJING AGRICULTURAL UNIVERSITY
Kit for simultaneously detecting classical strains and variant strains of porcine reproductive and respiratory syndrome virus and detection method thereof
ActiveCN101928782AAccurate detectionQuick DiscriminationMicrobiological testing/measurementMaterial analysis by electric/magnetic meansVariant strainRespiratory syndrome virus
The invention discloses primer pairs for simultaneously detecting classical strains and variant strains of porcine reproductive and respiratory syndrome virus, which have nucleotide sequences shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4. The invention also discloses a kit which comprises the primer pairs for simultaneously detecting the classical strains and the variant strains of the porcine reproductive and respiratory syndrome virus, and a method for simultaneously detecting the classical strains and the variant strains of the porcine reproductive and respiratory syndrome virus by adopting the kit. The primers in the kit for detecting the porcine reproductive and respiratory syndrome virus have the advantages of higher specificity, sensitivity and stability, and capacity of providing a rapid, sensitive and specific method for detecting the porcine reproductive and respiratory syndrome virus.
Owner:山东兴安智慧科技有限公司
Porcine epidemic diarrhea virus (PEDV) fluorescent quantitative PCR primer and probe
InactiveCN106947832AImprove featuresIncreased sensitivityMicrobiological testing/measurementDNA/RNA fragmentationPorcine epidemic diarrhoea virusMicrobiology
The invention discloses a fluorescent quantitative PCR identification and detection method of a PEDV classical strain and a PEDV variant strain. According to the method, based on the S1 gene of a PEDV classical strain and a PEDV variant strain, two specific probes are designed, and a pair of specific primers is designed on the periphery of the probes. The cDNA of a representative strain CV777 of PEDV classical strain and a representative strain CH / HNLY of PEDV variant strain is taken as the target fragment of template amplification, recombinant plasmids are established, taken as the standard substance, and used to establish a standard curve, and according to different report genes of two probes, different strains are identified and detected. The provided method has good specificity, sensitivity, and repeatability, and can be used to identify and detect a PEDV sample in clinic.
Owner:HENAN ACAD OF AGRI SCI +1
COVID-19 subunit vaccine as well as preparation method and application thereof
ActiveCN113321739AEasy extractionEasy to purifySsRNA viruses positive-senseViral antigen ingredientsAdjuvantReceptor
Fusion protein RBD-hFc / RBD-His obtained by combining a novel coronavirus (SARS-CoV-2) spike protein receptor binding structural domain (RBD) with a human immunoglobulin hFc domain or His tag through a genetic engineering means is convenient to extract and purify, stable and controllable in quality, short in time consumption and capable of being produced on a large scale. A vaccine composition prepared from the fusion protein and an adjuvant can increase the solubility and stability of a vaccine, enhance the immunogenicity of the vaccine and prolong the half-life period of the vaccine in vivo. The fusion protein and the vaccine composition thereof can inhibit replication and transmission of SARS-CoV-2 wild type and / or variant strains or prevent the SARS-CoV-2 wild type and / or variant strains from settling in a host, so that novel coronavirus pneumonia caused by the SARS-CoV-2 wild type and / or variant strains can be effectively prevented and / or treated.
Owner:广东克冠达医药科技有限公司
Porcine epidemic diarrhea virus variant strain and inactivated vaccine and application thereof
ActiveCN108486067AConvenient prevention and controlImprove securitySsRNA viruses positive-senseViral antigen ingredientsEpidemic diarrheaMicroorganism
The invention discloses a porcine epidemic diarrhea virus variant strain and an inactivated vaccine and application thereof. The invention discloses an isolated porcine epidemic diarrhea virus variantstrain PEDV-HB with the preservation number of CGMCC No. 14889. The invention further discloses a use of the variant strain PEDV-HB in preparation of a vaccine for preventing porcine epidemic diarrhea. The invention also discloses a vaccine composition for preventing porcine epidemic diarrhea. The vaccine composition comprises a prophylactically effective amount of the inactivated porcine epidemic diarrhea virus variant strain PEDV-HB and pharmaceutically acceptable adjuvants. The inactivated vaccine prepared from the porcine epidemic diarrhea virus variant strain PEDV-HB has good safety andgood immune effects, and can better prevent and control porcine epidemic diarrhea. The isolated porcine epidemic diarrhea virus strain PEDV-HB can be used as a candidate vaccine strain for preventionand control of porcine epidemic diarrhea.
Owner:黑龙江正康生物技术股份有限公司
Method for high yield, prematurity and dwarfing cultivation of jatropha curcas
InactiveCN101663996AImprove site conditionsPromote growthPlant growth regulatorsBiocideBiodiesel feedstockBiodiesel
The invention discloses a biodiesel raw tree, in particular to a method for high yield, prematurity and dwarfing cultivation of jatropha curcas. Plants with excellent biological and economical properties are selected out of numerous wild jatropha curcas or cultivated excellent variant strains; furthermore, by preferential selection, individual system breeding and artificial cultivation, domestication and breeding for many years, the plants are cultivated to excellent tree species; subsequently, by adopting adaptive tests for many years and multi-technologies, the jatropha curcas bears fruits in the transplanting year and can bear fruits more than five times per year; the oil content of the kernel of the jatropha curcas is 58.2-64.4%, the dry seeds per plant is 2-3kg, the yield per Mu in the transplanting year is 200-300kg (equivalent to the output of general wild adult tree after 3-4 years), and the yield per Mu can achieve 600-1000kg after growing up.
Owner:北京国林能源科技有限公司
Monoclonal antibody, vaccine composition thereof and application thereof
ActiveCN106188280AStrong neutralizing activityBroad-spectrumImmunoglobulins against virusesAntiviralsMonoclonal antibodyVariant strain
The invention provides a Pseudorabies virus resisting monoclonal antibody, a vaccine composition containing the monoclonal antibody and an application of the vaccine composition. The monoclonal antibody has very high neutralizing potency and can be used for effectively neutralizing swine classical and variant strains of Pseudorabies virus; and the vaccine composition containing the monoclonal antibody has the characteristic of broad spectrum, application objects are free from limitations to animal species, and the preventing and treating efficacy (effect) of display of swine and canine animal experiments is excellent.
Owner:LUOYANG PULIKE WANTAI BIOTECH +1
PRV (pesudorabies virus) gB monoclonal antibody and application thereof
The invention belongs to the technical field of immunobiology and particularly relates to application for a patent of a PRV (pesudorabies virus) gB monoclonal antibody and application thereof. The antibody is obtained by screening PRV variant strains HeNZK-2014 and PRV gB proteins with hybridoma cells. The hybridoma cells capable of stably secreting the PRV gB protein monoclonal antibody is obtained through screening, so that the gB protein monoclonal antibody with high potency is obtained, a finished kit is prepared from the antibody, and a matched one-step competitive ELISA detection method is established. Through a series of verification for specificity, sensitivity and repeatability, the result indicates that according to the PRV gB antibody provided in the application and the established one-step competitive ELISA detection method, a comparatively accurate and comparatively stable detection result is achieved, the result can be better used for detection and evaluation of the PRV gB antibody, and the foundation is laid for preventing and controlling a PRV.
Owner:HENAN CENT FOR ANIMAL DISEASE CONTROL & PREVENTION
Porcine epidemic diarrhea virus variant strain, and isolated culturing method and applications thereof
InactiveCN105400744AEffective means of preventionSsRNA viruses positive-senseViral antigen ingredientsMicroorganismDisease
The invention discloses a porcine epidemic diarrhea virus variant strain, and an isolated culturing method and applications thereof. The porcine epidemic diarrhea virus variant strain is named as CH / ZMDZY / 11, and is preserved in China General Microbiological Culture Collection Center; and preservation number is CGMCC NO.10893. The porcine epidemic diarrhea virus variant strain can be used for preparing an inactivated vaccine which can be used for preventing porcine epizootic diarrhea effectively, so that an effective control method is provided for controlling porcine epizootic diarrhea caused by porcine epidemic diarrhea virus variant strains.
Owner:HENAN AGRICULTURAL UNIVERSITY
Reagent kit special for testing high pathogenicity pig replication and syndrome virus variation strain
InactiveCN101220397AStrong specificityIncreased sensitivityMicrobiological testing/measurementHighly pathogenicPcr method
The invention provides a special kit for detecting highly pathogenic porcine reproductive and syndrome virus mutation strain. The highly pathogenic porcine reproductive and syndrome virus mutation strain is detected by self-designing specific primers PSX1 / PSX2, extracting virus RNA and carrying out reverse transcription into cDNA and utilizing a PCR method; the size of the estimated amplification segment of an amplification mutation strain is 404bp and an amplification traditional PRRSV amplification segment is 494bp. Compared with the prior art, the special kit has strong specificity and sensitivity, and the coincidence rate can achieve more than 90 percent compared with a virus separation and IPMA method. The amplification results of the primers to hogcholera virus, pseudorabies virus and porcine parvovirus are negative, which can distinguish a NSP2 mutation strain and a traditional strain; the method can detect the PRRSV with 12.8ng / L, thus the invention can be widely used for the clinical detection of the highly pathogenic porcine reproductive and syndrome virus mutation strain.
Owner:INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
Pseudorabies virus passage attenuated strain and application thereof
ActiveCN110387354ALow toxicityImprove securityViral antigen ingredientsInactivation/attenuationAttenuated strainToxin
The invention provides a pseudorabies virus passage attenuated strain and an application thereof, and belongs to the field of vaccines of animal medicine. The pseudorabies virus passage attenuated strain is a pseudorabies virus LA2017 strain, and the preservation number is CGMCC No.18170. The invention also provides an application of the pseudorabies virus passage attenuated strain to preparationof pseudorabies vaccines, and a vaccine using the pseudorabies virus passage attenuated strain as an active component. The LA2017 strain is a natural deletion attenuated strain, the virulence is notably reduced, and when new-born piggies are inoculated with the LA2017 strain, no adverse reactions are caused. The strain is obtained by a passage attenuated method, so that the biological security risk is low. After weaned highly-susceptible piggies are subjected to primary inoculation of the vaccines prepared from the LA2017 strain for 7 days, 100% of protective effects can be achieved, and the immunization persistent period can achieve 5 months. The vaccines not only can prevent pathogenesis but also can prevent toxin expelling, and purification of pseudorabies virus variation strains can beextremely facilitated.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Method for breeding drought-resistant hybrid rice
InactiveCN104429934AExtended genetic distanceEasy to assemblePlant genotype modificationVariant strainDNA
The invention discloses a method for breeding drought-resistant hybrid rice. The method comprises the following steps: introducing total DNA of barnyard grass into a restorer R and a maintainer line B, planting the introduced DNA progeny material, harvesting seeds of a single plant to obtain seed rice D1, planting each single plant seed of the D1 in a single plant line area by 10-15 lines, respectively performing mixed harvesting on the single plant to obtain seed rice D2, planting each single plant seed of the D2 by 20 lines, performing single plant harvesting on variant strains of leaf type, ear type and plant type character donor barnyard grass so as to obtain seed rice D3, performing conventional planting on single plant D3, screening to obtain a single plant with good characters so as to obtain seed rice D4, carrying out backcrossing on the selected maintainer line and 650A as well as the D4, thereby obtaining the offspring D5 with C4 plant resources. The method disclosed by the invention is simple and rapid, and the bred rice variety is excellent in drought resistance and has a high economic value.
Owner:CHONGQING ACAD OF AGRI SCI
PCR method of multiple primer, its reaction liquor and application for preparing detection reagent
InactiveCN1536088AReduce or eliminate complexityImprove featuresMicrobiological testing/measurementFermentationPcr methodNon targeted
The present invention relates to a PCR method of multiple primer, its reaction liquid and its application in preparation of microbial detection reagent, specially relates to that in PCR process two denaturation temp. of 92-97 deg.C and 65-87 deg.C are adopted to respectively make initial 2 or 3 circulation or other subsequent circulation so as to overcome the defect of existent multiple PCR which can form non-specific product. Said invention utilizes the proper product design and adoption to two denaturation temperatures so as to obviously reduce the possibility of that non-target product early-synthesized by PCR can participate in subsequence. Said invention is applicable to preparation of single-tubule multiple PCR quick detection reagent, and has extensive application for detecting various viruses.
Owner:徐定邦 +1
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