55 results about "Animal virus" patented technology

The invention discloses a recombinant humanized IV-type collagen M2 or M4, a nucleic acid molecule sequence for coding the recombinant humanized IV-type collagen M2 is shown as SEQ ID NO.1, and a nucleic acid molecule sequence for coding the recombinant humanized IV-type collagen M4 is shown as SEQ ID NO.3. The invention also discloses a recombinant humanized IV-type collagen M4. The recombinant humanized IV-type collagen M2 or M4 provided by the invention adopts a genetic engineering technology and a biological fermentation method to express and purify the recombinant humanized IV-type collagen, has the advantages of single component of a final product, few impurities, no animal virus, no immune risk, convenience in industrialization and the like, can effectively improve the proliferation and migration activity of cells, and can be used for preparing the recombinant humanized IV-type collagen. The method can be widely applied to the fields of food, cosmetics, health care products and medical and mechanical products.

The invention relates to the technical field of virus detection, in particular to a method for enhancing animal virus detection sensitivity through optical fiber SPR probe surface modification, which comprises the following steps: S1, optical fiber SPR probe surface pretreatment: obtaining a hydroxylated surface; s2, gradient nanostructure modification: forming a gradient aperture surface topological structure; s3, carboxyl functionalization: immersing the substrate into a 3-aminopropyltriethoxysilane / methacrylic acid mixed solution, and constructing a carboxyl activation layer on the surface; s4, fixation of the aptamer: realizing directional fixation of the aptamer through EDC / NHS mediated carboxyl-amino coupling reaction; s5, non-specific site closing: carrying out cross-linking closing on the non-binding sites by adopting a thioacetamide / polydopamine composite solution; s6, virus detection: acquiring the surface refractive index variation through a phase modulation type SPR detection system; according to the invention, accurate fitting and quantitative correction of virus concentration are realized, and the stability and accuracy of detection are improved.

The invention relates to the technical field of animal virology, in particular to a target sequence for detecting the virulence of a largemouth bass frog iridovirus and application of the target sequence. The invention provides a target sequence for detecting the virulence of a largemouth bass frog iridovirus. The nucleotide sequence of the target sequence is as shown in SEQ ID NO: 1; the invention also provides a primer pair for detecting the virulence of the largemouth bass frog iridovirus. The detection method provided by the invention can efficiently and accurately distinguish the virulent strain and the virulent strain of the largemouth bass frog iridovirus, the detection time can be completed within 1.5 hours, a powerful support is provided for accurate judgment of the virulence of the largemouth bass frog iridovirus, and an effective means is also provided for screening the virulent strain and the virulent strain of the largemouth bass frog iridovirus.

The application discloses a kit for detecting equine infectious anemia virus antibody and belongs to the field of animal virus antibody detection. The kit comprises equine infectious anemia virus p26-gp90 recombinant protein, mouse IgG and goat anti-mouse IgG, and the equine infectious anemia virus p26-gp90 recombinant protein is composed of an amino acid sequence shown in SEQ ID NO. 1. The kit is used for detecting equine infectious anemia virus antibody, is convenient and fast, has high sensitivity, has no cross reaction with other pathogens, has high specificity, has great clinical significance and wide application prospect.

The invention belongs to the technical field of biological medicine, and discloses an aquatic animal antigen system and application thereof. The preparation method comprises the following steps: carrying out carboxylation treatment on bacterial cellulose to prepare carboxylated bacterial nano-cellulose, and then carrying out grafting reaction on the carboxylated bacterial nano-cellulose and an aldehyde group polymer to prepare surface modified nano-cellulose (namely poly (N-isopropylacrylamide) grafted carboxylated bacterial nano-cellulose). The surface-modified nanocellulose is used as a carrier and is chemically connected with a virus antigen protein (namely recombinant G protein) to prepare an antigen system (namely BNC-G) carrying the virus antigen protein. According to the antigen system, virus antigen protein can be delivered into aquatic animal bodies through soaking immunization, the effect of preventing and controlling aquatic animal virus diseases is achieved, and the antigen system is suitable for production of aquatic animal SPF seedlings and preparation of aquatic animal immune protection preparations.

The application discloses a primer probe set for simultaneously detecting multiple frog viruses, a kit and application thereof, and belongs to the technical field of animal virus detection.The primer probe set for detecting frog viruses comprises a primer group with the sequence shown in SEQ ID NO.1-2 and a probe with the sequence shown in SEQ ID NO.3.According to the D5family NTPase gene sequence of the Ranavirus member, the primer and the probe are designed, and through optimization of reaction conditions, a method for detecting frog viruses based on qPCR is finally established.The method has the characteristics of high specificity, good repeatability, high sensitivity, simple operation and reliability, and has good market application prospect and popularization and application value.

The present invention relates to the field of diagnostic detection of animal virus antibodies, and specifically provides a method for detecting African swine fever virus antibodies and its application. First, the present invention screens the dominant epitope regions of the structural proteins p30, p54, and p72 of African swine fever, tandemly expresses and purifies the three dominant epitope regions using an Escherichia coli expression system, and uses the purified protein as a coating antigen to establish an indirect ELISA method for detecting clinical swine sera. The present invention makes up for the deficiencies of existing domestic and foreign detection methods, and will be more conducive to the monitoring of antibody levels and prevalence generated by ASFV vaccine immunization and wild virus infection.

This invention discloses a sample collection device for animal virus detection, relating to the field of virus collection technology. It includes a device housing with a rotating structure inside. The rotating structure includes a main shaft rotatably mounted inside the housing, a winding shaft fixedly connected to the upper end of the main shaft, and a sterilization structure on the outer surface of the winding shaft. The sterilization structure includes an adjusting rope wound around the outer surface of the winding shaft at one end, a connecting bracket fixedly connected to the other end of the adjusting rope, a sterilizing lamp on the outer surface of the connecting bracket, a guide wheel movably mounted on the outer surface of the adjusting rope, a fixed bracket between the guide wheel and the inner wall of the device housing, and a slide rail fixedly connected to the outer surface of the inner wall of the device housing. This structure solves the problems of existing animal virus detection sample collection devices where the support plate holding the collection bottle is fixed and cannot rotate, making it inconvenient to remove the collection bottle, and the sterilization lamp used for sterilization has a fixed position and poor sterilization effect.

The invention discloses an attenuated, broad-spectrum and pancreatin-independent porcine epidemic diarrhea virus recombinant strain as well as a construction method and application thereof, and belongs to the technical field of animal virology and genetic engineering. According to the present invention, the 894-993 site amino acid sequence of the PEDV AH2012 / 12 variant strain S protein is transformed into the 894-993 site amino acid sequence of the JS2008 classical strain S protein through the CRISPR / Cas9 system and the homologous recombination method, and the recombinant virus rAH2012 / 12-894-993aaJS2008 is obtained through the rescue; compared with a parent virus AH2012 / 12, the recombinant virus rAH2012 / 12-894-993aaJS2008 has the advantages that the proliferation titer in cells is obviously improved, the toxicity is highly weakened, a cross-protective neutralizing antibody aiming at the G1 type and the G2 type of the PEDV can be induced, and the virus strain can be used for fundamental research and vaccine research and development.

The application relates to the technical field of animal virus molecular detection, and particularly discloses a multiple detection method for ASFV, PCV2 and PCV3 suitable for rapid real-time fluorescent PCR. Specific primers and TaqMan probes are designed according to the B646L gene of ASFV and the ORF1 gene conservative regions of PCV2 and PCV3, and the sequences are shown as SEQ ID NO:1-9. The primers and probes are optimized: avoiding strong GC anchoring at the 3' end of the primers, continuous AT series and G base at the 3' end of the probes, and shortening the length of the probes to adapt to rapid real-time fluorescent PCR. The minimum detection limit of the three viruses is not higher than 5 copies per muL, the detection is completed within 30 minutes, there is no cross reaction with 11 common pig pathogenic agents, the coefficient of variation is less than 2%, the sensitivity is high, the specificity is strong, the method is rapid and convenient, and is suitable for rapid diagnosis of mixed infection of ASFV, PCV2 and PCV3.

The application relates to the technical field of animal virology, and particularly discloses a GIIa type porcine epidemic diarrhea virus (PEDV) strain and application. The PEDV strain is named as LZ202401, and was preserved in the China Center for Type Culture Collection on October 15, 2024, with a preservation number of CCTCC NO:V202488. The strain provided by the application is a strong pathogenic PEDV strain, and after infecting piglets, the piglets appear vomiting and diarrhea conditions, so that the piglets are relatively slow in development, rough in hair, seriously dehydrated, depressed in spirit and constantly tremble. The inactivated vaccine prepared by using the strain has good immunogenicity and can be used for preventing porcine epidemic diarrhea caused by PEDV.

The application discloses a kind of based on water body virus load and fish body tissue virus load correlation grouper nervous necrosis virus nondestructive testing method.The method is through optimizing the concentration condition of virus in aquaculture water, realize the efficient concentration and quantification of water body virus load, then combine water-water aquaculture animal virus load correlation model, indirectly and nondestructively assess the virus infection state of aquaculture animal.The application detects water environment instead of detecting animals itself, realizes nondestructive, dynamic, early warning monitoring of the health status of aquaculture animal population, with the advantages of low cost, simple operation, frequent implementation, etc., and has important application value in aquatic disease prevention and control, good seed selection and biological safety management.

The application discloses a monoclonal antibody of immune serum antibody for O-type foot-and-mouth disease virus and application thereof, and relates to the technical field of animal virology and animal infectious disease detection. The application uses vp1 protein as an immunogen, uses polypeptides containing B cell antigen epitopes and inactivated antigens as screening origins respectively, screens out monoclonal antibodies capable of competing with O-type foot-and-mouth disease virus inactivated vaccine and synthetic peptide vaccine immune background serum, and establishes an O-type foot-and-mouth disease virus solid-phase competitive ELISA kit, which greatly shortens the detection time and improves the sensitivity, specificity and stability of the kit.

The application provides an avian PCV3 gene and application thereof, and belongs to the technical field of animal virology and immunology. The application provides an avian PCV3 gene for the first time, which is named aPCV3, and the nucleotide sequence of the aPCV3 gene comprises any one of SEQ ID NO. 1-2 or a sequence with a homology of more than 90% with SEQ ID NO. 1 and 2. The virus strain containing the aPCV3 gene has a high virus titer and can be stably passaged for multiple times. The inactivated vaccine prepared from the virus strain containing the aPCV3 gene and the subunit vaccine prepared from the Cap gene or the Rep gene in the aPCV3 gene have a significant protective effect on poultry such as chickens, ducks and geese. The ELISA detection plate prepared from the Cap gene or the Rep gene in the aPCV3 gene can accurately detect PCV3 and has high specificity and repeatability.

The invention is applicable to the technical field of animal virus detection, and provides a PVC2 and PRRSV (porcine reproductive and respiratory syndrome virus) double colloidal gold test strip and application thereof. Ferritin nanocage fusion protein of PCV2 and PRRSV is taken as a capture antibody; a gold-labeled PCV2 monoclonal antibody and a gold-labeled PRRSV monoclonal antibody are used as detection antibodies, and the gold-labeled PCV2 monoclonal antibody and the gold-labeled PRRSV monoclonal antibody are obtained by labeling a specific monoclonal antibody composition by using a gold labeling technology and treating the specific monoclonal antibody composition. The prepared double colloidal gold test strip is high in specificity, low in production cost, easy to operate and visual in result, PCV2 and PRRSV infection can be rapidly identified and diagnosed on site at the same time, and the double colloidal gold test strip has wide market application prospects.

The utility model relates to the technical field of scraping devices, in particular to a live pig tongue coating and saliva scraping device for animal virus detection. Comprising a scraping piece and a handle, the handle is composed of a threaded rod and a threaded sleeve, one end of the threaded rod is fixedly provided with a rotary sampling assembly, the rotary sampling assembly comprises a connecting frame fixedly arranged at one end of the threaded rod, the connecting frame is rotationally provided with a rotating cylinder, and a plurality of connecting rods are evenly distributed around the rotating cylinder; the number of the scraping pieces is multiple, the multiple scraping pieces are connected to the multiple connecting rods in a buckled mode respectively, and each connecting rod is provided with two limiting assemblies for limiting the positions of the scraping pieces. The utility model provides a live pig tongue coating and saliva scraping device for animal virus detection, which can collect a plurality of samples at one time and can adjust the length of a handle.

The invention discloses a dual real-time fluorescent quantitative PCR (polymerase chain reaction) detection method for simultaneously detecting porcine hemagglutinated encephalomyelitis virus (PHEV) and porcine teschovirus (PTV), and belongs to the technical field of animal virus molecular biological detection. Aiming at the problems of similar clinical symptoms, high fatality rate and lack of synchronous detection and identification means after the suckling piglets are infected by PHEV and PTV, a specific primer pair and a probe are designed and screened through sequence comparison based on conserved regions of genomes of the two viruses. The detection method provided by the invention can realize qualitative and quantitative analysis of the two viruses, has the advantages of high sensitivity, high specificity and simplicity and convenience in operation, can quickly identify single infection or mixed infection of the two viruses, and provides an effective technical means for accurate detection in laboratories and epidemiological investigation.

The application discloses primers and a probe for real-time fluorescent quantitative PCR detection of a porcine epidemic diarrhea virus G2c subtype and application thereof and belongs to the field of animal virus molecular biology testing methods. The application compares S genes of different gene subtype strains of the porcine epidemic diarrhea virus, designs a pair of primers and a Taqman-MGB probe on the basis of a mutation site specific to the G2c subtype strain, and proves that the primers and the probe can effectively distinguish the G2c and other PEDV gene subtype strains through virus liquids and clinical samples of different gene subtype strains of the PEDV, meanwhile, the primers and the probe have no cross reaction to other viruses of the porcine enteric diarrhea syndrome, and the detection sensitivity of the kit can reach 5 copies / muL. The application can realize specific and sensitive detection of the G2c subtype, and has a positive significance for diagnosis and epidemiological investigation of the porcine epidemic diarrhea virus.

The application discloses a kit for detecting antibodies of canine ehrlichia, and belongs to the field of animal virus antibody detection.The kit comprises canine ehrlichia MAP2-P30-gp19 recombinant protein, mouse IgG and goat anti-mouse IgG, and the MAP2-P30-gp19 recombinant protein is composed of an amino acid sequence shown in SEQ ID NO.1.The kit is used for detecting the antibodies of canine ehrlichia, and has the advantages of convenience, high sensitivity, no cross reaction with other pathogens, high specificity, great clinical significance and wide application prospect.

The invention provides an EIDD-1931 sustained-release preparation and a preparation method thereof, and belongs to the technical field of medical preparations, and the EIDD-1931 sustained-release preparation is prepared by uniformly mixing EIDD-1931, hydroxypropyl methyl cellulose K15M, polyvinylpyrrolidone K23-27, carbomer, sodium bicarbonate, a flow aid and a filler and tabletting. By preparing the antiviral drug sustained-release agent for cats, the half-life period of EIDD-1931 in plasma of cats is prolonged, the EIDD-1931 only needs to be administered once in one day, the drug feeding difficulty of pet owners is reduced, and the compliance of the pet owners is improved, so that the treatment effect after animal virus infection is improved, the recurrence rate is reduced, and the animal survival treatment and animal welfare are improved.

The invention provides a porcine circovirus type 5 Cap protein, a virus-like particle, an elisa plate, an ELISA kit and application thereof, and belongs to the technical field of animal viruses. The invention provides a porcine circovirus type 5 Cap protein with an amino acid sequence as shown in SEQ ID NO. 1. The porcine circovirus type 5 Cap protein provided by the invention has the advantages of stable structure and good antigenicity, can be specifically combined with a circovirus type 5 antibody in a serum sample, and can be self-assembled into 60 virus-like particles. The ELISA plate and the ELISA kit for detecting the porcine circovirus type 5, provided by the invention, have the advantages of strong specificity and high sensitivity, and can accurately detect whether a blood sample contains the porcine circovirus type 5 or not. The porcine circovirus type 5 virus-like particle provided by the invention has the advantages of high yield, good uniformity and strong stability, and can be subsequently prepared into a porcine circovirus type 5 subunit vaccine.

The invention belongs to the field of animal virus nucleic acid in-vitro detection, and discloses a kit and a method for detecting a Batai virus based on RPA-CRISPR / Cas12a. Specific RPA primers (SEQ ID NO.1-2) and gRNA-3 (SEQ ID NO.5) are designed, Cas12a trans-cleavage is completed within 15 min at 37 DEG C after isothermal amplification is performed for 30 min at 39 DEG C, and a result is read through a fluorescence or immunochromatography test strip. The sensitivity is 18.9 copy / mu L, compared with traditional qPCR, the time is shortened, and cross reaction with congeneric viruses is avoided. The method is suitable for non-diagnostic purpose epidemiological monitoring and animal quarantine screening.

The invention relates to animal virology and genetic engineering technologies, and aims to provide a recombinant porcine epidemic diarrhea virus with enhanced replication capability. The virus is obtained by modifying a porcine epidemic diarrhea virus PEDV, and the amino acid sequence of nucleocapsid protein (N protein) of the virus is shown as SEQ ID NO: 2. The strain of the virus is preserved in the China Center for Type Culture Collection, the preservation name is rcPEDV P120-NT139A, and the preservation number is CCTCC NO: V202562. According to the invention, threonine at the 139th site of nucleocapsid protein of PEDV is a key modification site influencing PEDV infection, and the new strain can be successfully infected in PEDV infection model verification experiments of IPEC-J2 and Vero cells; the strain shows stronger replication ability in a PEDV infection model verification experiment of IPEC-J2 cells, the production difficulty is reduced, and the strain can be further used for preparing a porcine epidemic diarrhea virus inactivated vaccine.

The application discloses a detection kit capable of distinguishing porcine Senecavirus infection or vaccine immunization, and belongs to the technical field of animal virus antibody detection. VP2-VP3-VP1 and 3AB-3C recombinant proteins used in the application have stronger antigenicity and diagnostic sensitivity when used for detecting porcine Senecavirus, can effectively distinguish natural infection from vaccine immunization, and thus realize accurate detection. In addition, the detection method also has good specificity, analysis sensitivity, repeatability and reproducibility, has a wider application range, and has important application value.

The invention relates to the technical field of biological medicine and animal virus prevention and control, and discloses a PRRSV (porcine reproductive and respiratory syndrome virus) nucleocapsid (N) protein targeted mini sticky body, a mini sticky body composition and application thereof, the mini sticky body is selected from binder-1, binder-2 or binder-3, and the sequences of the binder-1, the binder-2 and the binder-3 respectively contain amino acid sequences shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The mini sticky body composition is prepared from at least two of a binder-1, a binder-2 and a binder-3. The mini-sticky body or the mini-sticky body composition can be specifically combined with PRRSV nucleocapsid (N) protein, and proliferation of the PRRSV is inhibited.

The invention relates to a Cas13b protein and application of the Cas13b protein in inhibiting viruses in eukaryotic cells, and belongs to the technical field of biology. The Cas13b protein is derived from riemerella anatipestifer, and the amino acid sequence of the Cas13b protein is as shown in SEQ ID NO. 1. The Cas13b protein has the advantages that the Cas13b protein is derived from riemerella anatipestifer; on the basis, a method for inhibiting an avian influenza virus (AIV), a porcine epidemic diarrhea virus (PEDV), a porcine transmissible gastroenteritis virus (TGEV), a porcine delta coronavirus (PDCoV) and a porcine reproductive and respiratory syndrome virus (PRRSV) in eukaryotic cells is utilized by the RaCas13b.

The present invention belongs to the field of biomedical technology, and discloses an aquatic animal antigen system and its application. In the present invention, bacterial cellulose is carboxylated to obtain carboxylated bacterial nanocellulose, and then it is grafted with an aldehyde polymer to obtain surface-modified nanocellulose (i.e., poly(N-isopropylacrylamide) grafted carboxylated bacterial nanocellulose). Using the surface-modified nanocellulose as a carrier, it is chemically linked with a viral antigen protein (i.e., recombinant G protein) to prepare an antigen system carrying the viral antigen protein (i.e., BNC-G). The antigen system can deliver the viral antigen protein into the body of aquatic animals through immersion immunization, achieving the effect of preventing and controlling viral diseases of aquatic animals, and is applicable to the production of SPF fry of aquatic animals and the preparation of immunoprotective agents for aquatic animals.

The invention relates to the technical field of traditional Chinese medicine preparations, and discloses a traditional Chinese medicine composition for reducing the incidence rate of viral diseases of animals and a preparation process of the traditional Chinese medicine composition. 780 to 820 parts of illicium verum; 850 to 900 parts of radix astragali seu hedysari; and 820-860 parts of madder. The preparation process comprises the following steps: (a) extracting honeysuckle with water; (b) carrying out reduced pressure distillation on illicium verum to extract volatile oil, and carrying out inclusion with hydroxypropyl-beta-cyclodextrin; (c) carrying out alcohol extraction on the astragalus membranaceus and the madder, and recovering ethanol; (d) combining the liquid medicine in the step (b) and the filter residues in the step (c), and decocting with water; and (e) mixing the extracting solution in the step (a), the clathrate compound in the step (b), the ethanol concentrate in the step (c) and the decoction in the step (d), adding auxiliary materials, filtering and sterilizing. The formula is synergistic; the process adopts shunt extraction, reduced pressure distillation and clathration, so that thermosensitive and volatile components are retained, extraction is complete, and the effect of reducing the incidence rate of viral diseases of animals by the composition is ensured.

The application provides an avian PCV2 gene and application thereof, and belongs to the technical field of animal virology and immunology. The application provides an avian PCV2 gene, which is named aPCV2, and the nucleotide sequence of the aPCV2 gene comprises any one of SEQ ID NO. 1-2 or a sequence with more than 90% homology with SEQ ID NO. 1 and 2. The virus strain containing the aPCV2 gene has the advantages of high viral titer and stable passage. The inactivated vaccine prepared by using the virus strain containing the aPCV2 gene and the subunit vaccine prepared by using the Cap gene or the Rep gene in the aPCV2 gene have significant protective effects on poultry such as chickens, ducks and geese. The ELISA detection plate prepared by using the Cap gene or the Rep gene in the aPCV2 gene can accurately detect the PCV2 antibody level in serum, and has high specificity and repeatability.

The invention discloses an acanthopanax compound polysaccharide empty capsule with immunocompetence and a preparation method thereof, and relates to an acanthopanax compound polysaccharide capsule and a preparation method thereof. The acanthopanax compound polysaccharide empty capsule with immunocompetence is prepared from compound polysaccharide, a film-forming agent, a gelling agent, a coagulant aid and a plasticizer, the compound polysaccharide consists of acanthopanax polysaccharide and corn straw polysaccharide. According to the method, synergistic development of the acanthopanax residues and the corn straw is innovatively proposed, the extraction residues of the acanthopanax roots are converted into high-added-value raw materials, and the comprehensive utilization rate of medicinal materials is increased; by introducing agricultural waste straw polysaccharide, resource utilization of agricultural waste is achieved, and environmental pollution caused by straw burning is reduced. By adopting plant-derived substances, the possibility of animal-derived virus pollution is avoided, so that the problem of animal virus pollution risk is solved. The prepared acanthopanax compound polysaccharide hollow capsule is stable in mechanical property and relatively stable in chemical property, and the dissolution characteristic of the medicine is guaranteed.