The invention discloses a formula of feed for Chilo suppressalis artificial feeding, a preparation method of the feed, and a large-scale feeding method of chilo suppressalis, and belongs to the technical fields of insect artificial feed and large-scale feeding. The large-scale feeding method of chilo suppressalis comprises the following steps of 1, preparing the feed from 600 to 900 parts of distilled water, 12 to 18 parts of agar, 8 to 16 parts of rice stem powder, 40 to 55 parts of cooked soybean powder, 28 to 45 parts of rice chaff powder, 18 to 28 parts of casein, 4 to 9 parts of yeast powder, 8 to 16 parts of cane sugar, 1 to 4 parts of vitamin C1, 0.5 to 3 parts of 21 Jinweita, 0.5 to 3 parts of vitamin E, 0.1 to 1.2 parts of choline chloride, 3 to 8 parts of sorbic acid and 3 to 8 parts of aureomycin, 2, introducing chilo suppressalis egg masses and checking up a rate of hatchability, 3, collecting pupas, and 4, colleting eggs of chilo suppressalis adults. The formula of the feed for Chilo suppressalis artificial feeding, the preparation method of the feed, and the large-scale feeding method of chilo suppressalis have the advantages of simple processes, low cost, labor saving and time saving characteristics, easiness of operation, and good flexibility for large-scale feeding.