281 results about "Instar" patented technology

The invention belongs to the field of microbial natural product pesticides, and particularly discloses a polyketone compound as well as a preparation method and application thereof. The compound disclosed by the invention is prepared from a fermentation crude extract of a strain of tobacco endophytic fungus Aspergillus japonicus TE-739D, and the compound disclosed by the invention is prepared from the fermentation crude extract of the tobacco endophytic fungus Aspergillus japonicus TE-739D. The chemical structure of the compound is identified through a modern spectroscopy technology, such as a high resolution mass spectrum HRESIMS and a superconducting nuclear magnetic resonance spectrum NMR, and the compound is named as japonidiene C. The invention further discloses a preparation method of the compound. The polyketone compound japondiene C has an obvious insecticidal activity on 2-instar larvae of spodoptera frugiperda, and can be used for preventing and treating the second instar larvae of the spodoptera frugiperda.

The invention discloses a chilo suppressalis indoor artificial feeding feed and a feeding and reproduction method, and relates to the technical field of insect feeding. By optimizing the proportion of the indoor artificial breeding feed for the chilo suppressalis and the breeding method, the method has the following technical effects that (1) the survival rate of larvae within 15 days reaches 100%, and the survival rate of the larvae is increased; (2) the optimized feed formula can meet the nutritional requirements of larvae in each stage, the taste is good, the feed attraction is strong, and the synchronous development is promoted; (3) the breeding efficiency is improved; (4) the disease and pest risk is reduced, and the disease occurrence rate is lower than 5%; and (5) ensuring the quality of the insect source: the obtained chilo suppressalis insect source is consistent in physiological state and neat in development, the insect body conversion rate reaches 69.6%, and the insect use requirements of large-scale experiments are met.

The application discloses a kind of cydia pomella larva's food lure composition and application thereof.It includes the following weight parts of component: isoquercitrin 0.02~0.07 parts, phlorizin 0.6~0.9 parts, cyanidin-3-O-galactoside 0.01~0.02 parts, delphinidin-3-O-galactose 0.05~0.12 parts.The food lure component provided in the application has low dosage and low price, can improve the behavior regulation effect of lure core on larvae, reduce the production cost, and is suitable for popularization and promotion.

The artificial feeding device comprises a breeding mechanism and a humidifying mechanism, the breeding mechanism comprises a breeding container, a water seepage hole is formed in the center of the bottom of the breeding container, a filter screen is arranged at the bottom of the breeding container, and a sandy soil layer is arranged on the upper side of the filter screen; the humidifying mechanism comprises a humidifying container, the lower portion of the breeding container is placed in the humidifying container, and a plurality of water is added to the inner bottom of the humidifying container. The artificial feeding device for the pteropsidae larvae is used for artificial feeding of the pteropsidae larvae, staged isolated breeding can be conveniently carried out according to the growth cycle of the pteropsidae larvae, the breeding environment can be controlled, sandy soil can be conveniently replaced in good time, the insect growth environment is protected, and the artificial feeding survival rate of the pteropsidae larvae is increased; the whole growth process of the larvae is conveniently observed, and the species of the larvae are accurately determined.

The invention discloses application of cordyceps javanica Bd01 and a dispersible oil suspending agent thereof to prevention and control of spodoptera frugiperda, and relates to the technical field of biology. The research finds that the cordyceps javanica Bd01 has a strong infection lethal effect on larvae of spodoptera frugiperda at all ages, the pupation rate and eclosion rate of the larvae can be remarkably reduced, and the breeding cycle of pests can be effectively blocked. The invention further develops a dispersible oil suspending agent, and the suspending rate is effectively improved through formula optimization. The dosage form takes soybean oil as a dispersion medium, does not contain harmful solvents such as methylbenzene, is free of dust pollution and is friendly to operators and the environment, and ascorbic acid is added to improve the ultraviolet resistance of spores and prolong the effective period of the pesticide effect. In addition, the preparation also has a certain control effect on caffeinidae, is wide in application scene, provides a new scheme for green control of spodoptera frugiperda, solves the problems of chemical pesticide resistance and residue, and accords with the development requirements of green agriculture.

The invention belongs to the field of microorganisms, and particularly discloses Aspergillus nomiae for preventing and controlling spodoptera frugiperda. The technical problem to be solved by the invention is how to prevent noctuid insects from harming plants. The invention provides aspergillus for preventing and treating noctuid insects from harming plants, the aspergillus is Aspergillus nomiae WNAN1, and the preservation number of the aspergillus nomiae is CGMCC (China General Microbiological Culture Collection Center) No.41741. The invention further provides a preparation method of the aspergillus nomiae. Experiments prove that the aspergillus has a relatively high fatality rate on third-instar larvae of the spodoptera frugiperda, and can be used for preventing and controlling the spodoptera frugiperda.

The invention discloses an RNA (Ribonucleic Acid) interference method for parasitic wasps in lepidoptera larvae, which is characterized in that RNA interference is carried out by using a microinjection method or a nano-material immersion method when the parasitic wasps in the lepidoptera larvae are not separated from hosts or are separated from the hosts to cocoon, so that an RNAi system of the parasitic wasps is successfully constructed, and the defects that the parasitic wasps are small in size in an egg stage, and the parasitic wasps are not damaged in the egg stage can be effectively overcome. And RNAi operation difficulty caused by cocoon wrapping in the pupal stage and short life in the adult stage is avoided. Moreover, the method disclosed by the invention is simple to operate and high in interference efficiency, provides a theoretical basis and a reference effect for further researching gene functions of the parasitic wasps, and has important significance on biological control.

The utility model discloses a device for ground beeltle hatching, and relates to the field of ground beeltle hatching, the device comprises a hatching box, one side of the hatching box is fixedly connected with a positioning rod, the outer wall of the positioning rod is fixedly connected with a controller, the inner wall of the hatching box is fixedly connected with a fixing rod, the outer wall of the fixing rod is fixedly connected with a heater, and the heater is fixedly connected with the hatching box. The inner wall of the incubator is provided with a transparent plate, and the inner wall of the incubator is fixedly connected with a limiting rod. According to the soil turning device, the motor is arranged to clamp and fix the turning frame, when the soil turning device is used, soil can be accurately and uniformly turned through the arrangement of the motor and the turning frame, the labor intensity of workers is greatly reduced, and the problem that the soil turning effect is uneven due to manual operation is also solved; by arranging the ground beetle egg mass box, the anti-escape frame is clamped and fixed, when the device is used, larvae are effectively prevented from escaping after shell breaking through the arrangement of the ground beetle egg mass box and the anti-escape frame, and the practicability of the device is improved.

The utility model relates to a full-automatic separation system for wet materials containing live insects. The full-automatic separation system comprises a tiled feeding device and a live insect separation device, the tiling and feeding device comprises a stock bin, a material distributing and conveying belt, a first frame and a dispersing and tiling mechanism. The live insect separating device comprises a heat preservation cover, a steel wire mesh belt, a second frame and a larva avoiding mechanism, and the discharging end of the distributing conveying belt is connected with the feeding end of the steel wire mesh belt. Wet materials are uniformly spread into a thin layer through the dispersing and spreading mechanism, so that the hiding of live insects caused by material accumulation is avoided, and the exposure rate of the live insects is obviously improved; through seamless connection of the material distribution conveying belt and the steel wire mesh belt, full-process automation of'tiling, separation and collection 'is formed, manual intervention is reduced, and the treatment efficiency is improved; the steel wire mesh belt intercepts wet materials, the insect receiving conveying belt collects live insects, physical separation of the live insects and the materials is achieved, and the separation efficiency is high.

This invention provides a device for hatching and rearing soybean hawk moth eggs and larvae. Specifically, the device includes a hatching cage base, an egg distribution plate above the hatching cage base, larval climbing posts, a larval feeding platform, and a larval feeding chamber. This invention also provides a method for rearing soybean hawk moth larvae using this device.

The invention discloses a method for breeding, separating and purifying nosema bombycis, which comprises the following steps: infecting 2-year-old silkworms for 6-12 hours by using nosema bombycis with the concentration of 1.0 * 10 < 5 > spores / mL to 5.0 * 10 < 5 > spores / mL, feeding the silkworms with clean mulberry leaves until the silkworms fall asleep at 4 years old, and gradually causing pebrine death when the silkworms grow at 5 years old; the method comprises the following steps: grinding dead silkworms to prepare grinding liquid, filtering the grinding liquid, and separating out filtrate enriched with nosema bombycis; centrifuging the filtrate to obtain a precipitate which comprises grey silkworm body tissues on the upper layer and white nosema bombycis on the lower layer, shaking the centrifuge tube by using a shaking table to release the grey silkworm body tissues to supernate, pouring out the supernate, and retaining the white nosema bombycis at the bottom of the centrifuge tube. According to the method, the nosema bombycis is bred, separated and purified according to the growth rules and characteristics of the bombyx mori and the nosema bombycis, a high-quality sample can be efficiently obtained, and the nosema bombycis is high in content and few in impurity.

This invention belongs to the field of agricultural and forestry biotechnology, and relates to a method utilizing... 60 A method to increase the oviposition rate of beet armyworm by Co-γ irradiation was used. Beet armyworm pupae within 12 hours of pupal instar were collected and subjected to effective accumulated temperature treatment. Then, for male beet armyworm pupae, [further methods were employed]. 60 Co-source irradiation treatment; irradiated male beet armyworm pupae and unirradiated female pupae were placed in an incubator to cultivate and emerge as adults. The adults were then transferred to adult rearing cages and supplemented with honey water for nutrition. This invention, through effective accumulated temperature treatment, selects a large number of uniformly developed male beet armyworm pupae as irradiation targets. While ensuring emergence rate and adult lifespan, it significantly increases the egg production of beet armyworms and the yield of test insects. The operation process is simple, safe, and reliable. It provides a large source of production insects for the convenient and rapid use of beet armyworms to produce the biological control agent nucleopolyhedrovirus, and for using the insect as a host to propagate natural enemy parasitic wasps.

The utility model relates to a modularization and expandability protaetia brevitarsis larva breeding device which comprises a breeding box, the upper portion and one side of the breeding box are open, an expansion assembly facilitating expansion of the size of the interior of the breeding box is installed in the breeding box, and the expansion assembly comprises an expansion frame. A mounting assembly facilitating combination of the multiple breeding boxes is mounted on one side of each breeding box. Through the arrangement of the expansion assembly and the installation assembly, the breeding space can be adjusted according to needs, a plurality of breeding boxes can be rapidly assembled vertically or transversely, various combination modes are provided, the breeding device adapts to different breeding space layouts, and the problems that a traditional breeding device is usually of a fixed size, and the size is too large are solved. The problems that the resource utilization rate is low, the breeding efficiency is not high due to the fact that the breeding space cannot be flexibly adjusted according to the number and growth stages of larvae, and the flexibility and large-scale development of breeding production are limited due to the fact that a traditional breeding device is difficult to expand rapidly and conveniently are solved.

The invention relates to a whole-course artificial cultivation method for bamboo borers. The method comprises the steps of female and male egg recognition and screening, artificial incubation, cultivation carrier preparation, larva transplantation, larva cultivation and the like. According to the method, the male and female eggs are accurately distinguished according to the appearances of the eggs, the high-quality female eggs are screened for incubation, and the technical defects of uneven matching of the male and female eggs, low mating rate, poor survival rate and limited yield in the existing natural cultivation are solved by means of controlling the incubation temperature and humidity, optimizing the cultivation carrier and transplanting conditions, artificially regulating the adult mating environment and the like; the survival rate of the bred bamboo borer eggs reaches 90% or above, the stocking survival rate of larvae also reaches 90% or above, the yield is 20 times that of an existing natural breeding technology, operation is easy, the cost is controllable, the method is suitable for large-scale popularization, the win-win situation of ecological protection and economic benefits is achieved, the method has extremely high practical value and market prospects, bamboo cannot be damaged by breeding, and disease and pest spreading is avoided. And economic benefits are achieved on the premise of protecting the natural environment, and the concept of green development is met.

The invention discloses an artificial feed for efficient propagation of low-age larva parasitic wasps of prodenia litura. The artificial feed is prepared from the following raw materials: agar powder, soybean meal, sweet potato vines with leaves, yeast powder, wheat germs, sodium benzoate, vitamin C, multivitamins, linseed oil, chlortetracycline hydrochloride soluble powder and the like. The artificial feed is used for feeding 2-5-year-old prodenia litura larvae parasitized by parasitic wasps, and the pupation rate and eclosion rate of the parasitic wasps can both exceed 93%.

The invention discloses a molecular sequence and a primer for sex identification of grassland caterpillars and application of the molecular sequence and the primer. The nucleotide sequences of the molecular marker are as shown in SEQ ID NO: 1 and SEQ ID NO: 2; wherein a nucleotide sequence as shown in SEQ ID NO: 1 is female, and a nucleotide sequence as shown in SEQ ID NO: 2 is male. The invention also provides a primer group for amplifying the molecular fragment, and the nucleotide sequences of the primer group are shown as SEQ ID NO: 3 and SEQ ID NO: 4. Wherein the size of a strip capable of being amplified by a female individual is 484 bp, and the size of a strip capable of being amplified by a male individual is 199 bp. According to the molecular marker and the primer group provided by the invention, sex identification can be carried out on grassland caterpillars, larvae, incomplete pupae and cocoons with unknown sex, the accuracy is high, the speed is high, and a reliable method is provided for monitoring the field sex ratio of the grassland caterpillars.

The present disclosure provides a container for the breeding of insects, bugs and fungi, comprising an upper side, a lower side, two long sides and two short sides, wherein at least one closable opening arranged in at least one of the two long sides of the container; and at least one air conditioning and venting device attached to at least one of the two front sides of the container. Another object of the present disclosure relates to an assembly of containers comprising at least two of the containers as described above. Another object relates to a system for the breeding of insects, bugs and fungi, comprising at least one container or an assembly as described above; and a plurality of stacked breeding boxes configured for accommodating in the inner part of the container. Another object of the present disclosure relates to a method for the breeding of insects, bugs and fungi, comprising the steps of: providing a container comprising a t least one closable opening arranged in at least one of the two long sides of the container; at least one air conditioning and venting device attached to at least one of the two front sides of the container; opening the at least one door; placing at least one stack stacks of breeding boxes comprising insect larvae, bug nymphae or fungi spores and a nutrition medium inside the container; adjusting temperature and humidity inside the container with the air conditioning and venting device.

The invention discloses a special membrane material for targeted trapping of macrobrachium larvae in water and a preparation method of the special membrane material, and belongs to the field of water environment biological pollution prevention and control. The membrane material is of a multi-layer composite structure and comprises a nanofiber membrane base material layer, an interface adhesion layer and a functional modification layer. The interface adhesion layer is bionic coacervate gel formed by curing hydrophobic polyelectrolyte and phytic acid; the functional modification layer is covalently grafted with a bionic adhesion protein active peptide fragment and a macrobrachium larva chemical pheromone. The material actively attracts larvae to approach through chemical pheromones, high-strength adhesion capture is achieved through bionic peptide fragments rich in aromatic and cationic amino acids, and trapping is integrated. The preparation method comprises the steps of electrostatic spinning of a base material, surface activation, gel coating, functional modification and the like. The material disclosed by the invention has high selectivity and high capture rate on the larvae of the Limnoperna fortunei, is environment-friendly, long-acting and stable, and is suitable for green prevention and treatment of fouling of the larvae of the Limnoperna fortunei in facilities such as water pipelines and pump stations.

The present invention provides compositions and methods for inducing suspended animation in neonate larvae of beneficial fly, particularly larvae of the Black soldier fly. During the suspended animation, the larvae show extended shelf-life and improved shipment durability. The suspended animation is reversible, and upon exposure to standard rearing conditions the larvae reach comparable maturity to corresponding non-suspended larvae.

The present invention relates to a device for releasing an insecticide suitable for controlling mosquito larvae. The device comprises a reservoir containing a preparation of at least one insecticide having larvicidal properties, wherein the reservoir comprises an outlet for releasing the preparation of the at least one insecticide. The device further comprises a gas-generating cell configured to build up a pressure by means of gas generation, wherein the device is configured such that pressure built up by the gas-generating cell causes movement of the preparation of the at least one insecticide out of the outlet of the reservoir. The invention also relates to method for controlling mosquito larvae, in particular by releasing a preparation of at least one insecticide having larvicidal properties, using a device according to the present invention.

The invention belongs to the technical field of disease and pest control, discloses application of tyrosine hydroxylase as a target spot in prodenia litura pest control for the first time, screens out a dopamine synthesis inhibitor AMPT aiming at tyrosine hydroxylase, and develops a technology for efficiently controlling prodenia litura. According to the technology, AMPT is injected into the fourth-instar larvae of the prodenia litura, and the melanin deposition of the exoskeleton of the larvae is reduced, the color of the body surface becomes light and the athletic ability is remarkably reduced by utilizing the dopamine synthesis inhibition function of AMPT. On one hand, reduction of melanin deposition weakens the environmental adaptability of pests, and the exposure risk of the pests in the natural environment is increased; on the other hand, the migration behavior of the pests is directly influenced by the decline of the exercise ability, and the population diffusion and hazard range of the pests are limited. The dual-action mechanism reduces the environmental adaptability and survival competitiveness of the pests, and provides a new strategy for green prevention and control.

The application discloses a high-quality and high-yield cultivation method of wasp pupa cordyceps, and belongs to the field of artificial cultivation technology of rare medicinal and edible fungi. The method selects frozen wasps at each day age of mature larva stage to metamorphic stage as main raw materials of culture medium, and the culture medium is comprehensive in nutrition and high in quality. The cordyceps cultivated by the method contains high medicinal and nutritional components such as cordycepin, amino acid and cordyceps polysaccharide, and the content of cordycepin is particularly outstanding. The application has high safety and is not affected by environmental factors. The application adopts a pipette injection method, and the culture medium is prepared through normal pressure sterilization or high pressure sterilization. The number of wasp pupae inoculated per hour is 20 times that of the prior art, and the bacterial contamination rate can reach 100%. The application solves the problems of labor-intensive, time-consuming and low bacterial contamination rate of the current manual injection method, shortens the time for the insect body to be fully covered with bacteria by more than one week, and increases the yield by more than 3 times. The application is convenient for large-scale production, and has novelty, creativity and wide application value.

The application provides a method for preventing and treating monochamus alternatus, which comprises putting male monochamus alternatus carrying Wolbachia into a target prevention and treatment area of monochamus alternatus not carrying Wolbachia. The method has low investment cost, is green, safe, environment-friendly, has no pesticide residue and high risk, conforms to the green prevention and control principle and needs of diseases and pests in the new era, is less affected by external temperature, humidity and other climate environments, is suitable for wide plots, is a new prevention and treatment method, is early in the prevention and treatment period, prevents and treats from the egg stage, reduces the double hazards of the larval stage dry woodworm and the pupal stage adult stage spreading pine wood nematode disease from the root, has good prevention and treatment effect, and can significantly reduce the egg hatching rate of monochamus alternatus by more than 90%.

The invention discloses a target gene capable of adapting to hunger stress of axyridis polyaxyridis, which is an HvarAKR1B1 gene for coding aldose reductase, and is used for detecting the hunger stress and comparing the gene expression and metabolite change of the axyridis polyaxyridis through transcriptomics and metabonomics technologies. The key gene for responding to hunger stress of the heterodylaea axyridis is screened by difference analysis and is verified by an RNAi (Ribonucleic Acid Interference) technology. After the gene is silenced, the survival rate, the eclosion rate and the survival rate from the 4-instar larva to the adult larva of the heterodylaea polyalaea are obviously reduced, and the RNAi-mediated gene reduces the hunger tolerance of the heterodylaea polyalaea. Therefore, the silence or overexpression of the gene can obtain the effect of effectively regulating and controlling the population quantity of the axyridis polyaxyridis, and the gene has relatively good popularization and application values.

The invention relates to the technical field of resource insect breeding, in particular to living larva separation equipment which comprises a separation device, the separation device comprises at least one layer of separation mechanism, the separation mechanism comprises at least two conveying mechanisms distributed in parallel, a gap is formed between every two adjacent conveying mechanisms, and living larvae can pass through the gaps. The conveying mechanism is driven by a power mechanism; an insect collecting area is arranged below the conveying mechanism, and an insect sand collecting area is arranged at the tail of the conveying mechanism in the conveying direction, so that the separation effect of larvae and insect sand is greatly improved, and the cleanliness of living larvae is improved.

The invention discloses a pine moth control method for pinus sylvestris, and belongs to the technical field of pine moth control, and the specific control method comprises the following steps: (1) overwintering generation control: turning a dry branch and fallen leaf layer around a trunk to expose underground overwintering pine moths, and reducing the number of overwintering generation pine moths; (2) prevention and control in a tree stage: killing tree larvae by using a pine moth prevention and control agent; (3) adult prevention and treatment: trapping the adult pine moths with black light to reduce the breeding amount and reduce the number of insects in the next year; (4) prevention and control in a tree leaving stage: killing overwintering pine caterpillars in the tree by using a pine caterpillars prevention and control agent; biological and physical combined prevention and control are utilized for relieving pinus sylvestris insect pests, and the problems that beauveria bassiana is prone to being affected by the environment and the action effect is unstable when preventing and controlling the pinus sylvestris are solved.

The invention belongs to the field of shellfish culture, and particularly relates to a method for efficiently promoting chlamys farreri larva attachment and metamorphosis through levodopa. Aiming at the problem of low attachment and metamorphosis rate in the current chlamys farreri breeding process, the invention provides the method for efficiently promoting the attachment and metamorphosis of the chlamys farreri larvae by using the chemical inducer levodopa, and a technical support is provided for improving the attachment and metamorphosis rate in the chlamys farreri seedling production process.

The present application relates to the technical field of insect feeding, in particular to a feeding method for feeding Spodoptera exigua larvae with switchgrass. The method comprises the following steps: placing 2nd instar larvae in a culture container, placing one larva in each culture container, putting 1-4 pieces of switchgrass leaf segments into the culture container every day, sealing the gap between the upper cover and the lower bottom of the culture container and leaving a ventilation opening, putting 2-4 pieces of switchgrass leaf segments into the culture container every day when the larvae grow to 3rd instar-4th instar, keeping the gap between the upper cover and the lower bottom of the culture container, putting 2-5 pieces of switchgrass leaf segments into the culture container every day when the larvae grow to 5th instar-6th instar, keeping the gap between the upper cover and the lower bottom of the culture container, and feeding the larvae until they pupate. The present application feeds Spodoptera exigua larvae individually, feeds the larvae with a specific amount of switchgrass leaf segments at different instars and controls the contact amount with air, thereby providing highly suitable food and optimal nutrition and feeding environment for the larvae and improving the survival rate of the larvae.

The invention belongs to the field of agricultural pest control, and particularly relates to a bHLH family key gene Sim and application thereof in grassland spodoptera frugiperda control. The invention provides a bHLH family protein Sim which is a protein composed of amino acids as shown in SEQ ID NO.2, and a gene Sim for coding the bHLH family protein, and a coding region of the gene Sim is a DNA (deoxyribonucleic acid) molecule with a sequence as shown in SEQ ID NO.1. By reducing the expression of the Sim gene, spodoptera frugiperda larvae can grow slowly and die, the egg laying amount of adults is remarkably reduced, even the adults do not lay eggs, so that the population number of the adults is effectively controlled, and the harm caused by pest outbreak is reduced. A novel environment-friendly high-specificity RNA pesticide is developed based on the gene, and a new solution is provided for pest control.

The invention belongs to the technical field of organic matter preparation, and provides an isoxazoline compound containing benzamide as well as a preparation method and application thereof.Chemical modification and molecular design are performed on a compound with an isoxazoline structure, a substituent group is introduced to N of an amide group, and the benzamide-containing isoxazoline compound is prepared. A series of high-efficiency compounds which can be used for killing insects or mites in agriculture or forestry and have excellent activity are obtained, and particularly, the compounds have very good biological activity on pests of lepidoptera, hemiptera and the like. When being used at an extremely low concentration, the compound disclosed by the invention also shows an excellent control effect on various pests or pest mites, has insecticidal or acaricidal activity in each stage (such as eggs, larvae (nymphs), pupae and adults) of the life cycle of the pests or pest mites, and has an excellent control effect on the pests or pest mites which have drug resistance to traditional insecticides or acaricides. And the composition also shows excellent control activity.