2166 results about "Instar" patented technology

Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Disclosed is a novel delta -endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against colcopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneur). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

The invention belongs to the field of environmental protection, relating to a method for processing food waste by using black soldier fly larvae and comprising the following steps: coarse crushing the food waste and then adding auxiliary materials to obtain culture materials for the black soldier fly larvae; and grafting black soldier fly spawn on the surface of the culture materials for the black soldier fly larvae so that the larvae hatched by the black soldier fly spawn acquires the culture materials for the black soldier fly larvae, thereby processing and cleaning the food waste. Before the black soldier fly larvae ages, a polypide collecting container is externally connected to the area for piling up the culture materials for the black soldier fly larvae and is filled with dry rice bran powder so as to guide to collect the aged black soldier fly larvae; and the rest of the processed culture materials for the black solider fly larvae is dried and then prepared into microbial organic fertilizer. The invention has strong processing capability, rapid processing speed compared with the common compost, the processing period is 4-5 days; after being processed, the volume of the food waste is reduced by 10-30%, the peculiar smell of the waste is lightened; and the invention can obtain excellent microbial organic fertilizer and insect bioprotein at the same time.

The invention discloses a method for artificially feeding black soldier flies, which comprises the following steps of: feeding adults, collecting eggs and incubating, feeding larvae, pupating and the like. The provided method for artificially feeding black soldier flies is a mature method for artificially feeding black soldier flies. The method is easy and effective, is easy to operate, is suitable for mass propagation and production of the black soldier flies, and lays a foundation for effectively utilizing the black soldier flies, such as treating refuse, changing waste into treasure or using larvae as feeds or baits.

The invention discloses a hermetia illucens breeding system and belongs to the technical field of green cultivation. The hermetia illucens breeding system comprises a culture pond and a ceiling arranged at the upper part of the culture pond; the culture pond is sequentially provided with a water absorption layer, a heating device and a grid layer from bottom to top; the heating device comprises a heating tape and a fixing frame for fixing the heating tape; a compost is put on the upper part of the water absorption layer; the heating device is located in the compost; the grid layer is located on a surface layer of the compost; a lifting device is arranged on the side of the culture pond; the lifting device drives the heating device to lift; and a heat preservation cover is arranged at the top of the culture pond. Hermetia illucens is bred by using the hermetia illucens breeding system, so that the survival rate of hermetia illucens larvae is high and the hermetia illucens is not affected by an external environment; furthermore, the hermetia illucens is easily separated from the compost and insect feces do not need to be cleaned, so that the cost of manpower and material resources is greatly reduced. In addition, the compost in the culture pond is digested and fermented in the hermetia illucens breeding process and can be directly returned to the field as an organic fertilizer, so that the hermetia illucens breeding system is economical and environmentally friendly.

A modified comb (10, 10A, 10B or 10C) for heating drone bee larvae and pupae in a hive (100) is described. The comb includes wires (11, 11A, 11B, 11C, 11D) for treat the drone larvae and pupae(P) to kill the mites (M) on the larvae and pupae. By this method, the mites are prevented from infesting the hive.

The invention provides a total artificial method for cultivating the cordyceps sinensis, and belongs to the field of the total artificial cultivation of the cordyceps sinensis. The method comprises the steps of: a. preparing a Chinese hirsutella suspension; b. acquiring, propagating and raising the host insect; c. infecting: taking the hepialus larva obtained by the step b, and soaking the suspension of the step a by a needle-shaped apparatus with the diameter which is less than 0.2mm at one end to prick the hepialus larva to complete the infection; and d. continuously raising. After the method is used, the improper mortality of the chrysalis can be reduced, and the eclosion rate of the hepialus is improved; the mating rate of the hepialus can be improved; and the infection rate of the Chinese hirsutell to the host hepialus larvae can be greatly improved, and the total artificial industrial production of the cordyceps sinensis can be realized.

The invention relates to a method for culturing river crabs, in particular to a method, by which the river crabs cultured in a pond or other water areas and become large-size commercial crabs. The method is characterized in that the method comprises the steps of purchasing crab larvae; culturing the crab larvae: the disinfection of the crab larvae incubating pond, the arrangement of the escape preventing fence, fertilization, water injection to the crab larvae pond, the float grass cultivation, the stocking of the crab larva, and the feeding of artificial food; the over-winter of first instar crab; the culturing of the second instar crab: the disinfection of the crab larvae incubating pond, the arrangement of the escape preventing fence, fertilization, water injection to the crab larvae pond, the float grass cultivation, the feeding of the first instar crab, and the feeding of artificial food; controlling the gonad development speed of the second instar crab: the artificial over-summer and the natural over-winter of the second instar crab; culturing the large-size river crabs: the feeding and management for the second instar crab, the artificial induction to the river crabs for exuviation and growth. The method is in favor of increasing the culture scale, the culturing yield and the economic benefit through controlling the gonad development speed of the second instar crabs and applying the technologies of culturing the large-size river crabs.

In an aspect, a system for breeding and harvesting insects is provided and includes an egg-producing chamber structure configured to receive insect pupae for pupation and to permit emerged adult insects to mate and oviposit insect eggs, at least one oviposition region in the egg-producing chamber structure configured to receive the insect eggs and apertured to permit at least one of the insect eggs and neonates of the insect eggs to pass therethrough, at least one larvae-growth chamber in communication with the at least one oviposition region so as to be configured to receive the at least one of the insect eggs and neonates of the insect eggs, wherein the larvae-growth chamber is further configured to permit the at least one of the insect eggs and neonates of the insect eggs to transition into larvae and to hold feed material for the larvae, a harvesting receptacle positioned to hold larvae, and an inclined surface positioned to receive larvae from the at least one larvae-growth chamber, and to provide a passageway for the larvae to travel to the harvesting receptacle.

The invention relates to an artificial feed of sesamia inferens walker as well as a preparation method and a feeding method thereof, belonging to the technical field of insect feeding. The artificial feed comprises soybean meal, corn meal, wheat germ, yeast powder, casein, cane sugar, honey, fresh wild rice shoots or fresh rice stems, ascorbic acid, cholesterol, choline chloride, Fiske salt, vitamin B complex, vitamin E, sorbic acid, methyl parahydroxybenzoats, 40 percent formaldehyde, 98.18 percent rice ketone, agal-agal and water. A long-term and successive feeding method of the sesamia inferens walker is carried out by adopting a wild rice shoots (young larva) and artificial feed (old larva until pupation). The fed larva of the sesamia inferens walker have development period of 24.8 days, larva survival rate of 76.6 percent, percentage of pupation of 87.7 percent, eclosion rate of 91.3 percent, egg laying amount of 101.9 for single adult and egg hatching rate of 88.8 percent. The invention has the advantages of low cost, practicability, simple preparation, and the like, and is suitable for massive, long-term and successive feeding of standard test sesamia inferens walkers.

The invention discloses a super flour weevil culture technology which comprises the steps of: (1) field selection and requirement: selecting a field which avoids wind, faces sunlight, has good ventilation and light transmission, is warm in winter and cool in summer, wherein the size of the field is determined by culture scale; (2) culture facility: providing a feeding box which is 800 mm long, 400 mm wide and 80 mm high and a culture frame which is generally 2m high, wherein a high layer is about 200 mm high so as to be convenient for operation, and each layer is 20-40mm wider than the feeding box; (3) culture temperature and humidity: controlling and adjusting temperature and humidity per se, wherein the optimal indoor temperature is 18-28 DEG C and the optimal humidity is 60-80%; and (4) culture method comprising the steps of: (1) flour weevil seed selection: selecting big and mature larvas as flour weevil seeds which has strong survival ability and can creep fast from larvas; (2) larva feeding refering to the period from larva hatching to pupal period; (3) pupa and adult culture mainly for providing seeds and multiplying, i.e. enabling adults to lay a large mount of eggs. By the super flour weevil culture technology, big larvas which can grow fast and has short culture cycle and high yield and great economic benefits can be obtained.

The invention provides a method for suppressing the gene expression of insects through feeding dsRNA, which belongs to the growth development field of adjusting and controlling insects through naturally feeding the dsRNA of the silenced and specific gene. The method selects a gene fragment SeA3 on chitin synthetase enzyme gene SeCHS-A, and studies the impact on the insects after the natural feeding. The method mainly comprises the steps: a proper expression vector L4440 is selected to construct a recombinant expression vector L4440-SeA3 with a purpose gene fragment SeA3; the recombinant vector is induced and expressed in specific recipient cell coliform bacteria HT115 (DE3) under vitro adaptive condition; and the recipient bacterium htLSA3 which expresses dsRNA is added into the feedstuff of the insects, the insect larvae are naturally fed with the feedstuff, the situation of the insects suppressing the gene expression is detected after the feeding, and the change of the phenotype of the insects is recorded. By adopting natural feeding to suppress the expression of the insect genes, the method explores new paths for controlling the insects.

Larviculture is performed using Parvocalanus sp as a feed for fish larvae. A system is described using tanks for growing Parvocalanus sp nauplii with a microalgae feed and transferring the grown Parvocalanus sp nauplii to tanks containing the fish larvae, where the functions of the tanks is interchanged. The Parvocalanus sp feed provide for higher numbers of larger juvenile fish and the rearing of larvae heretofore not reared in culture.

The invention discloses a wasp breeding method. The method comprises the steps of firstly, in autumn, putting female and male wasps having mating flight into a wasp breeding shed for mating, secondly, keeping the female wasps after mating in a dark place for hibernation, and in the spring of the coming year, putting the individual female wasps after hibernation in nesting boxes for waking, and finally, putting the nesting boxes in the wasp breeding shed so that the female wasps ovulate, and if the emergence number of larvae is 15-20, moving the nesting box to a field farm from the wasp breeding shed for breeding. As a result, the problem that the wild wasp population cannot be applied to the biological breeding field is solved, and the wasp can be well applied to the fields of forestry, crops and fruit trees, and therefore, the wasp breeding has the advantages of improving the yields of the forestry, the crops and the fruit trees while protecting the wasp specie, and also has relatively high direct economic benefit and wide market prospect.

The invention relates to the technical field of aquaculture, and specifically relates to a feed for siniperca chuatsi. The feed is prepared by the following raw materials by weight: 55-60 parts of corn, 20-22 parts of rye bran, 10-12 parts of peanuts, 20-25 parts of rapeseed meal, 6-8 parts of chironomidae larvae powder, 8-10 parts of small carp meal, 8-10 parts of loach dry powder, 6-8 parts of herba houttuyniae, 10-12 parts of white fish meal, 6-8 parts of escargot powder, 8-10 parts of dry sphagna powder, 5-6 parts of meat and bone meal, 6-8 parts of radish leaves, 0.5-1 part of rheum officinale, 1-2 parts of saponin, 1-2 parts of felwort, a proper amount of salt and 4-5 parts of a phagostimulant. In consideration of the carnivorous characteristic of the siniperca chuatsi, an abundance of raw materials such as small carp meal, loach dry powder, white fish meal and escargot powder are added in the prepared feed for siniperca chuatsi, so that enough nutrients are provided for the growth and development of the siniperca chuatsi. Traditional Chinese medicine components such as herba houttuyniae, rheum officinale and saponin are added, so that disease-resistant capacity of the siniperca chuatsi can be enhanced. Particularly, parasitic diseases can be prevented; morbidity of the siniperca chuatsi is reduced; and loss of culture benefits can be prevented.

A mosquito control trap taking advantage of the normal stages of development for a mosquito. The trap including stagnant water trapped within a container by a weeping platform floating on top of the water. A series of holes in the weeping platform provide exposure of the water to form brood cups in which female mosquitoes lay eggs. In order to develop through the larva and pupa stage the developing insects must enter the larger reservoir of stagnant water, since there is insufficient room in the brood cups. However, the holes are too small to permit escape of the adult mosquito, thus preventing any of the eggs from developing. This device provides a means for controlling mosquitoes without the use of pesticides and is especially suitable for use in lesser developing countries.

Artificial cultivation method of apis cerana queen, first preparing artifical stylobate of apis mellifera, and choosing a group of apis mellifera queen as feeding group for larva of apis cerana queen; putting the artifical stylobate of apis mellifera into a selected feeding group of apis mellifera group, taking the artifical stylobate out after cleaned by worker bee of the apis mellifera, transfering larva of worker bee in mother apis cerana group that is in need of cultivating queen to the artifical stylobate; at last putting the artifical stylobate into generation box of the selected apis mellifera feeding group for feeding larva of apis cerana queen, after 11 to 12 days the apis cerana queen stylobate is mature, taking out the apis cerana queen stylobate before the virgin queen going out the room, introducing the virgin queen to apis cerana mating group for following mating, so as to become a spawning queen. The superiority of the invention is: obtaining large amount of mature apis cerana queen stylobate or virgin queen for one time; because of adequent apis mellifera bee milk, bigger and stronger virgin queen can be cultivated; apis mellifera and apis cerana of different species can generates nutritious heterosis which provides important meaning for apis cerana species cultivation.

The invention provides a method for treating cow dung with Hermetia illucens to prepare an organic fertilizer. The method comprises the following steps: dehydrating cow dung with a dehydrater; adding swill or kitchen waste of which the addition amount is 10-15% of the mass of dehydrated cow dung; and then adding lactic acid bacteria dry powder for fermentation treatment for 1-2 days, wherein the addition amount of lactic acid bacteria dry powder is 0.1% of the mass of dehydrated cow dung; and finally adding 3-4-day old Hermetia illucens larva, culturing for 5-6 days at 30-35 DEG C under the condition that the air humidity is 75-85%, and separating out mature Hermetia illucens larva, thus obtaining an organic fertilizer. The method has the advantages of high treatment capacity, short cycle and low energy consumption in the treatment process, and can be used for realizing the resource utilization of cow dung and kitchen waste and changing waste material into things of value. In addition, since the nutritional value of cow dung is higher than that of horse dung and lactic acid bacteria are used for fermentation treatment, cow dung becomes loose and is improved in palatability, the intake rate of Hermetia illucens is increased and the yield of mature Hermetia illucens larva can be improved by 50% or above. By virtue of the method, the environment sanitation is improved and moreover organic fertilizer and bioprotein are produced; and thus, the method has a very bright application prospect.

The invention provides an artificial diet for Lepidopterous young larvae. The artificial diet for the Lepidopterous young larvae is prepared from the following raw materials in percentage by weight: 2.0 to 3.0 percent of soybean cake meal, 7.0 to 10.0 percent of corn flour, 2.0 to 3.0 percent of Saccharomyces cerevisiae Hansen, 7.0 to 10.0 percent of tomato sauce, 0.8 to 1.2 percent of sucrose, 1.2 to 1.4 percent of agar, 0.2 to 0.5 percent of sorbic acid, 0.05 to 0.1 percent of vitamin B complex, 0.2 to 0.4 percent of ascorbic acid, 0.1 to 0.2 percent of ethyl p-hydroxybenzoate, 0.2 to 0.4 percent of propionic acid and 71.0 to 77.0 percent of water. The invention also provides a method for preparing the artificial diet and application of the artificial diet. The artificial diet is low in manufacturing cost, easy to operate and high in practicability; and by the artificial diet and the raising method for raising the Lepidopterous young larvae, the survival rate of the larvae is high, the workload is low, and manpower and material resources are greatly saved.

The invention relates to a black stratiomyiid breeding device and method. The device comprises two spaces which are divided by a separation plate, wherein the space A is an adult breeding chamber and the space B is a larva breeding chamber; ventilation windows are arranged on the outer side faces of the adult breeding chamber and the larva breeding chamber; a timing switching door is arranged at the upper part of the separation plate; a triangular adult ovulation mechanism is arranged in the middle of the separation plate; the triangular adult ovulation mechanism is composed of an ovum receiving box and one layer of insect attractant coating on the upper surface of the ovum receiving box; the ovum receiving box is arranged in the larva breeding chamber; an ovum rotary collection device is arranged under the ovum receiving box; an animal excrement storage groove is formed in the bottom of the larva breeding chamber; the animal excrement storage groove is arranged under the ovum rotary collection device; the space A is a space which is composed of a partial transparent material at least; and the space B is a space composed of a light shielding material. By using the device to breed black stratiomyiid, the breeding efficiency is improved and the automatic collection and growth of ova are realized and the breeding cost is also reduced.

The invention discloses a benthic diatom culture method for growing seedlings and an abalone fry culture method, which relate to an abalone culture technology. The invention is characterized by comprising the following steps: (1) starting culturing algaes 45 days before pulling seedlings, keeping the culture water temperature at 14 DEG C and the illuminance between 20001 and 30001x, controlling the light between 10001 and 15001x at the last culture after screening and eliminating algae culture for one and half a month, and laying ovum after 7 days; (2) adding nutrient salt at primary culture,wherein the adding ratio of N:P:Si:Fe is 5:1:1:1(ppm); (3) laying ovums about 5 millions for each pool and carrying out micro inflation; (4) changing water regularly after the ovums are laid and changing water twice a measuring range which is 24 hours; (5) carrying out running water culture after the ovums swim for 5 to 6 days and are adhered; (6) controlling the illuminance within 10001x after larvas metamorphose; and (7) dumping the pool after treating copepods at about 30 days of the culture period, adjusting the illuminance between 15001 and 20001x and peeling off baby abalones after about45 days. The methods have the advantages of improving the yield and preventing and treating a plate stripping disease.

The invention relates to a larva breeding device. The larva breeding device comprises a support frame, a larva breeding container and a feeding mechanism, wherein the support frame comprises at least one layer of support frame body and a bottom support from top to bottom, the larva breeding container is arranged at the upper end of each support frame body and can rotate along the support frame body, inside of the larva breeding container is divided by at least two partitions, and an unloading hole is formed in the bottom end of the larva breeding container along one partition; the feeding mechanism comprises a stirred material temporary storage hopper and subarea pipes, the bottom end of the stirred material temporary storage hopper is arranged on the bottom support, the subarea pipes are arranged on a discharge pipe of the stirred material temporary storage hopper from top to bottom and arranged above the larva breeding container, the subarea pipes above the same larva breeding container are distributed circumferentially on the outer surface of the discharge pipe of the stirred material temporary storage hopper, and discharge holes are uniformly formed below the subarea pipes. With adoption of the structure, a large amount of labor cost is saved, and the production efficiency is increased.

The invention provides a breeding method of selenium enriched black soldier fly larvae. Inorganic selenium or organic selenium is added into feed of the black soldier fly larvae and the concentration of selenium content in the feed of the black soldier fly larvae is controlled at 10 to 80 mg every kg. The selenium is added into the breeding feed of the black soldier fly larvae to obtain the high selenium content of black soldier fly larva products and accordingly the functional value of the black soldier fly larvae is significantly improved. The selenium is combined with protein and polysaccharide organically in the black soldier fly larvae after bioconversion and accordingly the toxic and side effect and the gastrointestinal stimulation of the chemical selenium are eliminated and the selenium can be absorbed and utilized efficiently and securely.

The invention relates to a poison bait for exterminating red fire ants, belonging to the field of chemical insecticide, which comprises the components with the following weight percent: insecticide technical product 0.5-1.0%, attractant 40-48%, bait carrier 50-60%, preservative 0.5-1.0%, and the insecticide technical product adopts two components of A and B as assistant components, and attrahent adopts three components of animal albumin powder, edible vegetable oil and flavoring agent as assistant components. The inventive bait formulation mixing insecticide technical product and attrahent is strong in attractive effects, good in palatability, stable in field performance, large in moving load, the medicament in the ant group is evenly transmitted to each grade of the red fire ants, ergates can be killed directly, causing grubs to develop irregularly of die, causing queen ants to be sterile or killing the queen ants. The poison bait is friendly on environment, high in security, which is suitable for comprehensive control on the red fire ants in the field, fruit forest, home, school and the like.