45 results about "Instar" patented technology

The application provides a method for preventing and treating monochamus alternatus, which comprises putting male monochamus alternatus carrying Wolbachia into a target prevention and treatment area of monochamus alternatus not carrying Wolbachia. The method has low investment cost, is green, safe, environment-friendly, has no pesticide residue and high risk, conforms to the green prevention and control principle and needs of diseases and pests in the new era, is less affected by external temperature, humidity and other climate environments, is suitable for wide plots, is a new prevention and treatment method, is early in the prevention and treatment period, prevents and treats from the egg stage, reduces the double hazards of the larval stage dry woodworm and the pupal stage adult stage spreading pine wood nematode disease from the root, has good prevention and treatment effect, and can significantly reduce the egg hatching rate of monochamus alternatus by more than 90%.

The application discloses a bamboo Dichelus quadernarius nymph trap, an attracting and avoiding agent and an application method, and belongs to the technical field of forest protection science, which comprises a plug rod, a socket is fixed to the top of the plug rod, a slow-release bottle is installed on the top of the socket, diffusion sponges are evenly arranged on the outer side of the plug rod in a circumferential direction, a drainage groove is arranged in the inner top of the plug rod and is used for conducting the bottom end of the slow-release bottle and the diffusion sponges, and a mounting disc is horizontally rotationally installed below the socket at the top of the plug rod. The plug rod is fixed and inserted into a bamboo forest, the slow-release bottle is arranged on the socket at the top of the plug rod, the inside of the slow-release bottle is filled with an attracting agent, target nymphs are attracted, and the cross-shaped glue boards distributed on the side of the plug rod can capture the attracted nymphs, so that the pollution of chemical control to the environment and the excessive consumption of manpower of physical control are avoided, in addition, the slow-release bottles containing the avoiding agent are placed between adjacent devices, the attracting agent and the avoiding agent are used in cooperation, and the trapping efficiency is improved.

This application discloses an indoor artificial rearing method for the rice stem borer, belonging to the field of insect rearing technology. This method addresses the problems of drowning in young larvae and frequent disease outbreaks in older larvae caused by homogeneous rearing across all generations. It constructs a constant-temperature environment and prepares granular dry feed for young larvae and slightly dehydrated flake feed for older larvae based on differences in larval mouthparts. A heterogeneous three-dimensional rearing device is used. In the young larvae stage, a slow-release substrate at the bottom maintains a vertical humidity gradient, and layered feeding prevents mechanical damage to the larvae. In the older larvae stage, substrate-free bare-bottom rearing and full-volume replacement feeding are employed. Timely removal of filamentous netting blocks the breeding environment for soft rot. Furthermore, the method combines pupation induction with corrugated paper induction and non-contact moisturizing, humidification during the adult dark period to delay gelatinous hardening, and overall substrate replacement during the light period to achieve damage-free egg mass harvesting. This application, through precise age-based management, significantly improves the survival rate and population propagation efficiency of the rice stem borer across all generations.

This invention discloses a method for indoor artificial rearing of the coconut-boring rhinoceros beetle, belonging to the field of artificial insect rearing technology. The method includes the preparation of a compound feed and phased rearing management: the compound feed uses macadamia nut husks as the main ingredient, supplemented with corn flour, wheat bran, soybean meal, and other auxiliary materials, and is prepared through fermentation, mixing, and humidity control. During rearing, suitable temperature and humidity are controlled for the adult, egg, larval, and pupal stages respectively. Adults are supplemented with fruit, and larvae are given supplemental feed as needed. The feed is regularly changed, and the environment is cleaned. This invention features a scientifically formulated and controllable rearing condition, adapting to the growth needs of the coconut-boring rhinoceros beetle at each developmental stage, ensuring its normal growth and development, improving the rearing success rate and survival rate, and achieving stable indoor artificial rearing of the coconut-boring rhinoceros beetle. This provides a reliable insect source for the research and development of this medicinal and resource insect, and is practical.

The embodiment of the present application discloses a feeding method for improving the survival rate and weight of Polyphylla laticornis larvae in the process of processing and converting fungus dungs. The feeding method comprises the following steps: S1: obtaining the feces generated in the feeding process of 2nd instar and / or 3rd instar Polyphylla laticornis larvae; S2: obtaining the feces substrate formed by the feces; S3: putting the 2nd instar Polyphylla laticornis larvae into the feces substrate, and placing them in an environment with a temperature of 28±2 DEG C and a light cycle of L:D = 16h:8h for feeding for 10 days, and the substrate humidity is controlled at 50%-55%; S4: picking or screening the larvae fed for 10 days from the feces substrate, and putting them into fungus dung substrate for further feeding. The present application can improve the survival rate and weight of Polyphylla laticornis larvae in the process of processing and converting fungus dungs, improve the utilization rate of insect feces, simplify the pretreatment process of fungus dungs, and reduce the feeding cost. The feeding method is simple and feasible, convenient to obtain materials, and has a great promoting effect on the processing and converting fungus dungs industry of Polyphylla laticornis.

This invention discloses a precise and efficient breeding method for *Cyprinus dorsiflorus* based on habitat reconstruction. The breeding pond is divided into a parent mussel rearing area and a glochidia larva metamorphosis development area by a barrier wall consisting of an upper, switchable isolation net and a lower, fixed partition. The glochidia larva metamorphosis development area is constructed with a 20-mesh movable net forming two layers. After laying the substrate, mature parent mussels and host fish are introduced. The temperature is controlled at 22.0–24.0℃, and fertilization is promoted with a water flow of 0.08–0.15 m / s, while glochidia larvae are cultivated with a water flow of 0.06–0.10 m / s. Once the glochidia larvae in the brood pouch mature, the isolation net is opened, and the water flow is adjusted to 0.02–0.05 m / s to promote parasitism. Every 6–8 days, the parasitized and molted parent mussels and host fish are circulated and exchanged. After the molted mussels molt, they are threaded through the net and placed into the lower substrate for in-situ cultivation. This method achieves in-situ integration of fertilization, parasitism, and cultivation, significantly improving the parasitism rate and the survival rate of juvenile mussels. It is suitable for the preservation, propagation, and large-scale seedling cultivation of *Cyprinus cuspidatum*.

The application discloses a kind of anti-insect nets for shatian pomelo trees, including: fixing belt, buckle, support rod, unfolding rod, anti-insect net and edge elastic band, compared with existing anti-insect nets, the application can quickly complete installation and disassembly, and is installed on the trunk without being affected by the terrain, and can protect from the roots of the tree to the anoplophora chinensis and anoplophora chinensis larvae, the double-layer structure design of the anti-insect net can hook the feet of adult insects with the convex semicircular structure and serve as the second layer of protection of the outer net, and the outer net sticks the larvae or adult insects with small mesh fish silk net, effectively preventing anoplophora chinensis from damaging fruits.

This invention discloses HpHeat3 Genes and their application in controlling the teak moth. This invention introduces a synthetic teak moth through a feeding method. HpHeat3 The dsRNA of the gene is delivered into the teak moth via the nanocarrier SPc, which can specifically silence the teak moth's... HpHeat3 Gene expression inhibits the growth and development of teak moth larvae, prolonging their development cycle or even stopping them completely. The larvae are significantly smaller than those of normal growth and the mortality rate is significantly increased in the early larval stage, thus achieving the purpose of biological control. This method has the advantages of specificity, high efficiency and environmental protection in pest control. It is a new type of green and pollution-free teak moth control technology and has shown great application prospects for teak moth control.

This application discloses an indoor artificial rearing method for the rice stem borer, belonging to the field of insect rearing technology. This method addresses the problems of drowning in young larvae and frequent disease outbreaks in older larvae caused by homogeneous rearing across all generations. It constructs a constant-temperature environment and prepares granular dry feed for young larvae and slightly dehydrated flake feed for older larvae based on differences in larval mouthparts. A heterogeneous three-dimensional rearing device is used. In the young larvae stage, a slow-release substrate at the bottom maintains a vertical humidity gradient, and layered feeding prevents mechanical damage to the larvae. In the older larvae stage, substrate-free bare-bottom rearing and full-volume replacement feeding are employed. Timely removal of filamentous netting blocks the breeding environment for soft rot. Furthermore, the method combines pupation induction with corrugated paper induction and non-contact moisturizing, humidification during the adult dark period to delay gelatinous hardening, and overall substrate replacement during the light period to achieve damage-free egg mass harvesting. This application, through precise age-based management, significantly improves the survival rate and population propagation efficiency of the rice stem borer across all generations.

Disclosed are methods for inducing quiescence in black soldier flies (BSF), enhancing synchronization in their breeding cycles, and optimizing egg production. The methods, systems, and compositions include inducing quiescence during the larval stage of the life cycle of a black soldier fly (BSF). Also disclosed herein are methods of inducing 1:1 mating to generate inbred lines of BSF. Environmental controls for quiescence induction, optimized oviposition attractants, and tailored feed formulations to improve larval and pre-pupal survival rates are provided. These techniques support efficient propagation and utilization of BSFs for animal feed and sustainable products.

This invention discloses the chitin synthase 1 (LmCHS1) gene of the leafminer moth, its dsRNA, its synthesis method, and its insect-resistant applications. Through transcriptome analysis, specific primer pairs for the open reading frame of the LmCHS1 gene were designed and synthesized. The LmCHS1 gene sequence was obtained through gene cloning (nucleotide sequence: SEQ ID NO: 1, amino acid sequence: SEQ ID NO: 2). The gene fragment with sequence SEQ ID NO: 3 was selected as the RNAi target sequence. Primer pairs SEQ ID NO: 6 and SEQ ID NO: 7 with restriction enzyme sites were designed and synthesized. PCR amplification was performed, and a prokaryotic expression vector was constructed to induce dsRNA expression. Feeding leafminer moth larvae with dsRNA interfered with LmCHS1 gene expression. After 24 hours, abnormalities were observed in the larvae's body surface, with some larvae failing to complete molting or dying due to an inability to form a normal body surface after molting. After 96 hours, the larval mortality rate was 72.22%, compared to 40% in the GFP control group, demonstrating a significant effect. This invention has broad application prospects in the control of the leaf borer pest in forestry due to its high efficiency, specificity, and safety.

The utility model belongs to algae-eating insect cultivation technical field especially relates to algae-eating insect screening cultivation device, including incubator and support leg, the support leg fixed mounting is in the cultivation box bottom all around, the inside of cultivation box is provided with cultivation device, the cultivation device includes screening mechanism, washes screening mechanism, cleaning mechanism and aeration mechanism, the screening mechanism sets up in the inside of cultivation box, washes screening mechanism and set up in the back of cultivation box, the cleaning mechanism sets up in the inside of cultivation box bottom, the aeration mechanism sets up in the inside of cultivation box lower extreme. This algae-eating insect screening cultivation device, through setting up screening mechanism, through the starting motor through output shaft drive disc rotation, disc rotation drives mounting seat one eccentric rotation under the action of ball head rod, pulls screening box and reciprocates along the guide rod left and right and shakes, thereby makes the young algae-eating insect to pass through screening box, realizes the separation of larva and adult, prevents adult predation larva, improves the algae-eating insect cultivation efficiency.

The utility model relates to agricultural waste treatment equipment technical field especially based on light and heat dual drive's black soldier fly larvae and insect sand separation system, include: frame, upper layer separation bin and upper layer separation bin. Upper layer separation bin sets up in the frame top, including the netted conveyer belt of horizontal operation, the flattening scraping thin device of feed end, the light and heat source above netted conveyer belt and the screen mesh anti -blocking device on the return path, the lower layer collection bin sets up in the lower layer separation bin below, including the entity conveyer belt of horizontal operation. The flattening scraping thin device will viscous or too thick insect sand even spread thin, promote light and heat penetrability and larvae drill out efficiency, the screen mesh anti -blocking device passes through the brush penetration and cleans the conveyer belt screen mesh hole, avoids the screen mesh blockage and leads to the conveyer belt shutdown. The above based on light and heat dual drive's black soldier fly larvae and insect sand separation system can realize the efficient and stable separation of larvae and insect sand.

This utility model belongs to the field of forestry pest control technology, and particularly relates to a longhorn beetle trapping and killing device, including a column and a base. An observation mechanism is located in the middle of the outer surface of the column, and a trapping box is connected to the bottom of the observation mechanism. The outer surface of the trapping box is fixedly connected to two trapping entrances, with heights of 100cm and 50cm respectively. Trapping box one contains a bark crevic filled with a pheromone lure from the red-edged longhorn beetle. Trapping box two contains a metabolite mimicry agent of the sea buckthorn wood-boring moth larvae, specifically an artificially synthesized odor of its feces or molting hormone. This longhorn beetle trapping and killing device, with two different trapping entrances, can cover active groups at different times and heights, specifically capturing upward-migrating larvae and adults. It also accommodates the spatial competition characteristics of the two pests, expanding the trapping range and enhancing the trapping effect through their competitive relationship. By confusing the odors, it attracts more red-edged longhorn beetle adults, improving trapping efficiency.

This invention discloses the application of 2-bromopentane in attracting cutworm larvae. This invention discovers that 2-bromopentane has an attractive effect on cutworm larvae. Therefore, this simple compound can be used to trap cutworm larvae, overcoming the problems of high cost and difficult operation of other technologies, and providing a diverse selection of attractants for the monitoring and control of cutworm larvae. This invention is simple and flexible to use. The single-component attractant for cutworm larvae provided by this invention—2-bromopentane—can be applied for monitoring or used in combination with pesticides for the control of cutworm larvae, making it convenient to use.

The utility model belongs to insect artificial breeding technical field, concretely relates to a box drawer type device of rice moth feeding and oviposition integration, including vertical cabinet, three layers of drawers are arranged in vertical cabinet, top layer is adult catching drawer, middle is larva feeding drawer, bottom layer is adult oviposition drawer, larva feeding drawer has bottom, top is open, the top of adult catching drawer is gauze, bottom is the net board of pluggable, when the net board in adult catching drawer is drawn out, adult catching drawer and larva feeding drawer intercommunicate, when the net board is inserted into adult catching drawer, can intercept rice moth adult between gauze and net board, when adult catching drawer shifts to bottom layer, replaces adult oviposition drawer, the below of adult oviposition drawer is provided with insect egg collection unit, through the structural design of " larva feeding drawer is above, adult catching drawer is below", utilizes the habit of adult insect flying upward to light, makes it automatically fly into the adult catching drawer (gauze place) of feeding drawer from above after eclosion.

The present invention relates to a method for producing beetle oil enriched with vitamin D3, comprising a light treatment step in which at least one light source emits ultraviolet radiation in the direction of said beetle oil.

This invention belongs to the field of animal husbandry technology, specifically relating to a method for raising larvae of the deer slender-bodied stag beetle. The method includes the following steps: larvae are raised in lightly fermented sawdust (containing 25%–30% lignin) at 22°C–23°C to obtain third-instar larvae. These larvae are then removed and transferred to new lightly fermented sawdust at 19°C–20°C for three months. After another three months of raising in the same sawdust at 17°C–18°C, the late third-instar larvae are obtained. These third-instar larvae are then raised in new lightly fermented sawdust at 14°C–15°C until they reach a weight of 25g–30g, completing the larval rearing process. Using this method, a larval breakage rate of 0% can be achieved, with an average male adult body length of 92.00mm. This provides a standardized rearing process for the larvae of the deer slender-bodied stag beetle, effectively improving breeding efficiency.

This invention discloses a method for promoting in-situ fungal and insect infection of Cordyceps sinensis in the wild. The method involves selecting the native habitat of Cordyceps sinensis on the Qinghai-Tibet Plateau, dividing the area into cultivation units through fencing, digging shallow trenches within each unit and laying a moisture-retaining covering layer; preparing a suspension of Cordyceps sinensis budding spores or a mycelial homogenate suspension; releasing 2nd-4th instar ghost moth larvae into the shallow trenches during a time window when the average daily soil temperature is 4-12℃, and immediately injecting the fungal solution; backfilling with the original soil and covering with a shade net to maintain relative soil humidity; maintaining the shade cover for 7-15 days, then removing the shade net and proceeding with regular cultivation. This invention, through micro-topographical modification and micro-environmental control, achieves an in-situ infection rate exceeding 70% and a stable grass formation rate of over 55%, while fully preserving the authentic wild quality of Cordyceps sinensis. The method is simple to operate, low in cost, and suitable for large-scale promotion in plateau production areas.

This invention relates to the fields of organic waste resource utilization and insect breeding technology, specifically a matrix improvement method to reduce the risk of heavy metal accumulation in black soldier fly larvae. The method involves adding a calcium-based organic acid complex to a matrix of organic waste containing heavy metal pollution. The calcium-based organic acid complex is a citric acid-carbide slag complex. This invention effectively inhibits the absorption and accumulation of heavy metals by black soldier fly larvae by adding a specific proportion of the calcium-based organic acid complex to a simulated kitchen waste matrix containing heavy metals, while simultaneously promoting larval growth, improving feed conversion rate, enhancing antioxidant capacity, and improving the intestinal microbial community structure.

Methods and compositions for non-lethal biological sampling of insect larvae are described herein, as are methods and compositions for determining the sex of an insect larvae.

This invention discloses a method for identifying the dietary range of reed wasps based on multi-technology integration. This method addresses the differences in nutritional requirements between adult and larval stages of reed wasps by integrating four technologies: field observation of flowers, microscopic identification of pollen on the wasp's body surface, microscopic identification of mixed pollen masses in nest cells, whole-genome sequencing of mixed pollen masses in nest cells, and bioinformatics analysis. Through standardized sample collection, extraction, identification, and sequencing analysis procedures, combined with cross-validation of multiple technologies, it achieves accurate identification of the dietary range of reed wasps throughout their entire life cycle. Simultaneously, it clarifies the key parameters and judgment criteria for each technical step, constructs a reference sequence library specific to tropical island plants, and verifies the high consistency of results among different technologies using the Kappa coefficient. This invention avoids the limitations of identifying dietary habits using a single technology, achieving full-coverage identification of the adult-larval life cycle of reed wasps with high accuracy and reliable results, providing a standardized technical framework for dietary research on solitary pollinating insects in tropical islands.

Short title: Process for valorizing insect larvae, composition obtained and use in animal feed. Process for preparing an insect larva composition from a medium containing live insect larvae and substrate residue particles, comprising at least one air separation step to obtain said insect larvae on the one hand and substrate residue particles having a density different from the density of the insect larvae on the other hand, and a heat treatment step of said insect larvae at a temperature ranging from 85°C to 105°C for a duration ranging from 1 to 15 minutes, and insect paste obtained by this process. Short figure: None

The application discloses a kind of noctuidae larva food attractant and preparation method, food attractant and application, including volatile component 0.0028-0.0042% by weight, nutrient component 2.3-3.5%, deionized water is supplemented to 100%.The application adopts volatile component 0.0028-0.0042%, nutrient component 2.3-3.5% combination, nutrient component provides the internal motivation of larva feeding, and volatile component provides long-distance attractant signal, and the dual mechanism of "near end attraction+remote guidance" is formed by the synergistic effect of both, has significant attractant effect to cotton bollworm, pyrausta nubidalis, beet armyworm, spodoptera exigua and other various noctuidae larvae.Has broad-spectrum attractant effect to noctuidae larvae, can reduce the number of times and the amount of drug, avoids pesticide abuse, so that pests appear drug resistance.To human and livestock, natural enemies and natural environment have no toxic side effects, and are safe to environment without pollution.

This invention relates to a method for propagating the Japanese ladybug and its application, belonging to the field of insect population propagation technology. The method for propagating the Japanese ladybug includes introducing Japanese ladybugs to lay eggs on eggplant leaf discs, which hatch into larvae; feeding Mediterranean mealybug eggs in a culture dish to obtain adult Japanese ladybugs; mating and egg-laying in a concentrated manner to obtain Japanese ladybug eggs; and repeating this cycle to complete the propagation of the Japanese ladybug. The Japanese ladybugs propagated by this invention show significantly improved growth, development, and reproduction, with a significantly increased survival rate, and their predation ability against whiteflies is comparable to that of natural populations.

The present application belongs to the technical field of insect feeding, and provides a kind of artificial feed for small line angle wood borer larvae, and its preparation method and application.The artificial feeding special feed includes the following raw materials by weight: white waxwood shavings 90-110 parts, soybean powder 25-35 parts, corn flour 10-20 parts, agar powder 3-7 parts, sucrose 2-4 parts, yeast powder 2-4 parts, cholesterol 0.3-0.7 parts, potassium sorbate 0.3-0.7 parts, methyl paraben 0.3-0.7 parts, ascorbic acid 0.3-0.7 parts, soybean oil 3-7 parts, and water 90-110 parts.Through three months of feeding test, it is proved that the growth rate and weight gain of group B larvae are significantly better than those of other groups, and the growth state of the experimental group with added oil and fat is obviously better, which provides an efficient technical solution for the large-scale artificial feeding of small line angle wood borer larvae, and provides a stable insect source for related biological research and prevention and control technology development.

This invention discloses a sampling device for the crawling stage of shellfish larvae, comprising a hollow tube, a one-way valve at the front end of the hollow tube, a sample storage bottle located below the front end of the hollow tube, and an adjustable air valve and piston located at the rear end of the hollow tube. The use of this device will greatly assist shellfish seedling cultivation, saving time and manpower: for large seedling ponds, sampling personnel no longer need to enter the water; for deep water, there is no need to drain the water for sampling; the sampling area is precisely controllable; there is no longer concern that the water volume in the collected sample will be much larger than the target material (bottom mud, sediment, etc.); at the same time, the impact of sampling on the seedling water body is minimized.

An insect farming installation comprises: a module comprising continuous profiled containers, each continuous profiled containers being configured to be filled with substrate containing larvae, wherein the continuous profiled containers are vertically superimposed to one another, and wherein at least some of the containers are configured to be filled with substrate in a way adapted to maximize an age difference of the larvae contained in the substrates respectively filling two consecutive containers, and wherein the containers are at least partially made with a thermally conductive material.