493 results about "Instar" patented technology

The invention relates to the technical field of biological control, and discloses a hyphantria cunea biocontrol bacterium and application thereof, the biocontrol bacterium is classified and named as Entomortierella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella chlamydosporella The hyphantria cunea biocontrol bacterium strain provided by the invention can obviously inhibit the survival activity of hyphantria cunea, the death rate of hyphantria cunea larvae can be increased to 96.7% after 7 days of treatment, and the hyphantria cunea larvae can die in the pupal stage and cannot be subjected to eclosion, so that the hyphantria cunea biocontrol bacterium strain can be used for preventing and treating hyphantria cunea, is typical biological prevention and treatment, can avoid the problems of drug resistance, environmental pollution and the like caused by chemical prevention and treatment, and has broad application prospects. Good application prospects are realized.

The invention discloses a cordyceps sinensis intelligent bionic cultivation control system and method based on the Internet of Things, and the method comprises the steps: collecting the temperature and humidity, gas concentration, illumination intensity and larva behavior data of a cordyceps sinensis matrix through a sensor network, and carrying out the fusion through an edge calculation gateway, and generating a dynamic environment map; constructing a plateau ecological digital twinborn model based on the dynamic environment map in combination with historical meteorological data and a host-strain interaction rule, and generating a staged control time sequence; based on a staged control time sequence, temperature and humidity coupling compensation is carried out through a feedforward-feedback composite algorithm, and actuator linkage is adjusted through fuzzy PID; aI vision is used for identifying a larva infection window period and accurate spraying is carried out, and hypha infection is monitored in combination with a near infrared spectrum to dynamically adjust the CO2 concentration; and constructing an LSTM (Long Short Term Memory) model to predict the growth risk in combination with a dynamic environment map and insect body monitoring data, and actively intervening in an abnormal environment. According to the invention, high-precision, dynamic and intelligent regulation and control of the growth environment of cordyceps sinensis are realized.

The invention relates to a device for use in large-scale industrial insect farming. More in particular, the invention relates to an insects transport device for transporting live insects from a first location to a predetermined second location, the insects transport device comprising a gas guiding unit, a gas discharge member and a feeder arrangement, wherein the insects transport device is configured to receive live insects such as freshly hatched neonate larvae, for example of black soldier fly, or mites, wherein the live insects are directly taken up in a laminar flow of gas after exiting the feeder arrangement in a free fall under influence of gravitation such that the live insects do not contact any surface of the insects transport device, and while in said gas are transported to a predetermined location in the insects transport device. Furthermore, the invention relates to the use of the device in industrial insect farming, such as large-scale farming of black soldier flies or mites. The invention also relates to a method of dosing a pre-selected number of live insects, wherein for example live insects are dosed which are essentially of the same age (e.g. within an age difference of 1 second-1 minute), such as freshly hatched neonate larvae. In addition, the invention relates to a single dose of a pre-selected and determined number of neonate larvae obtained with the method, wherein larvae have a seconds to at most a few minutes larvae-to-larvae age difference within the single dose.

The invention discloses a device special for an unmanned aerial vehicle to throw dastarcus helophoroides, and relates to the technical field of agricultural unmanned aerial vehicle dastarcus helophoroides throwing, the device comprises a main body shell, a buckling cover, an auxiliary block, a filling port and a plugging piece, the buckling cover is buckled on the main body shell, the auxiliary block is fixedly connected to the main body shell, and the filling port is fixedly connected to the main body shell; through the design of the anti-adhesion quantitative and uniform throwing assembly, the biological prevention and control effect can be remarkably improved, the device accurately controls the throwing number and uniformity of dastarcus helophoroides, and it is ensured that a proper amount of dastarcus helophoroides can be distributed around each forest tree threatened by longhorn beetle pests in a target area; the composition can prey on larvae and pupae of longhorn beetles more comprehensively and efficiently, the population density of the longhorn beetles is greatly reduced, insect pest diffusion is restrained from the source, the health and stability of a forest ecological system are effectively protected, and the biological control effect on major tree trunk borers such as monochamus alternatus, anoplophora glabripennis and mulberry beetles is greatly improved.

The invention belongs to the field of microbial natural product pesticides, and particularly discloses a polyketone compound as well as a preparation method and application thereof. The compound disclosed by the invention is prepared from a fermentation crude extract of a strain of tobacco endophytic fungus Aspergillus japonicus TE-739D, and the compound disclosed by the invention is prepared from the fermentation crude extract of the tobacco endophytic fungus Aspergillus japonicus TE-739D. The chemical structure of the compound is identified through a modern spectroscopy technology, such as a high resolution mass spectrum HRESIMS and a superconducting nuclear magnetic resonance spectrum NMR, and the compound is named as japonidiene C. The invention further discloses a preparation method of the compound. The polyketone compound japondiene C has an obvious insecticidal activity on 2-instar larvae of spodoptera frugiperda, and can be used for preventing and treating the second instar larvae of the spodoptera frugiperda.

The invention relates to the technical field of hermetia illucens larva breeding, in particular to a hermetia illucens larva drying and sorting device and method.The hermetia illucens larva drying and sorting device comprises a drying box internally provided with a heating device used for drying fresh hermetia illucens larvae; the separating device is connected to the outlet end of the drying box and comprises a first conveying belt, a plurality of separating plates are arranged on the first conveying belt, and the separating plates and the first conveying belt do not interfere with each other; the recovery device comprises a chain type larva taking spoon device, a funnel conveying piece in butt joint with the chain type larva taking spoon device and a second conveying belt located at the bottom of the funnel conveying piece, and dried hermetia illucens larvae fall on the second conveying belt through the funnel conveying piece and are distributed on the second conveying belt at intervals; the blowing screening device comprises a blowing device and an image acquisition device, the image acquisition device is close to one end of the funnel conveying piece, the blowing device is fixed on the side surface of the second conveying belt, and the control system controls the blowing device to start according to image acquisition information. The device and the method are simple in structure and high in sorting precision.

The invention discloses a chilo suppressalis indoor artificial feeding feed and a feeding and reproduction method, and relates to the technical field of insect feeding. By optimizing the proportion of the indoor artificial breeding feed for the chilo suppressalis and the breeding method, the method has the following technical effects that (1) the survival rate of larvae within 15 days reaches 100%, and the survival rate of the larvae is increased; (2) the optimized feed formula can meet the nutritional requirements of larvae in each stage, the taste is good, the feed attraction is strong, and the synchronous development is promoted; (3) the breeding efficiency is improved; (4) the disease and pest risk is reduced, and the disease occurrence rate is lower than 5%; and (5) ensuring the quality of the insect source: the obtained chilo suppressalis insect source is consistent in physiological state and neat in development, the insect body conversion rate reaches 69.6%, and the insect use requirements of large-scale experiments are met.

The invention relates to the technical field of insect grease extraction, and discloses a method for extracting hermetia illucens grease through hot pressing. The method comprises the following steps: (1) cooking hermetia illucens larvae in hot water at 40-75 DEG C for 5-15 minutes, and cleaning to obtain a larva material I; (2) drying the larvae I at the temperature of 60 to 80 DEG C, so as to obtain a larva material II with the water content of 55.0 to 67.5 weight percent; (3) mechanically squeezing the larva material II at the temperature of 80-100 DEG C to obtain slurry; and (4) centrifugally separating the slurry into an oil phase, a water phase and a solid phase, and recovering the oil phase to obtain the hermetia illucens grease. The method provided by the invention can significantly improve the yield and quality of the hermetia illucens grease extracted by hot pressing, and can effectively solve the problems of low high-valued utilization rate and low grease extraction efficiency of large hermetia illucens breeding factories.

The invention provides a breeding method of cantheconidea furcellata, and belongs to the technical field of insect feeding. The invention provides a breeding method of cantheconelaconus furcellatus. The breeding method comprises the step of feeding the cantheconelaconus furcellatus with frozen larvae of spodoptera litura. According to the method, fresh lapidoptera furcellata larvae are used for feeding lapidoptera furcellata, second-year-old lapidoptera furcellata nymphs can only eat second-year-old and lower-year-old lapidoptera furcellata larvae, and in the age, the lapidoptera furcellata nymphs do not die in a large number. According to the method, the survival rate of the low-age nymphs of the cantheconidea furcellatus can be remarkably increased by feeding the cantheconidea furcellatus with the frozen cantheconidea furcellatus. Meanwhile, the frozen older podoptera litura larvae are used for feeding the cantheconidea furcellata, so that the use amount of the cantheconidea furcellata is greatly reduced, mass feeding is facilitated, the labor efficiency is improved, the labor cost is saved, and large-scale feeding is facilitated. The breeding method is suitable for large-scale breeding of the cantheconidea furcellatus, so that manual rapid large-scale breeding and long-term subculture breeding of the cantheconidea furcellatus are realized, and a new way is opened up for controlling noctuidae pests which are difficult to control.

The invention discloses a method for artificially breeding white moth gathering cotesia sinensis and applying the white moth gathering cotesia sinensis to biological control of fall webworm. According to the method, the key factors such as the optimal age, the optimal bee inoculation proportion and the optimal temperature of the hyphantria cunea larvae which are hosts for artificially breeding the cotesia cunea are determined, the parasitism rate and the offspring number of the cotesia cunea are effectively increased, the breeding period is shortened, and the bee breeding efficiency is improved. Meanwhile, an optimal scheme for prolonging the shelf life of the cotesia sinensis gather through low-temperature storage of the cocoons or newly-emerged adults is also defined. The new natural enemy product breeding application technology provided by the invention is specially applied to biological prevention and control of the fall webworms in the larval stage, not only enriches the types of existing fall webworm natural enemy products, but also provides a more comprehensive solution for biological prevention and control of the fall webworms, can reduce the excessive use of chemical pesticides, and is beneficial to environmental protection. And a powerful technical guarantee is provided for balance and health of an ecological system.

The application discloses a kind of cydia pomella larva's food lure composition and application thereof.It includes the following weight parts of component: isoquercitrin 0.02~0.07 parts, phlorizin 0.6~0.9 parts, cyanidin-3-O-galactoside 0.01~0.02 parts, delphinidin-3-O-galactose 0.05~0.12 parts.The food lure component provided in the application has low dosage and low price, can improve the behavior regulation effect of lure core on larvae, reduce the production cost, and is suitable for popularization and promotion.

The invention belongs to the field of fall webworm control, and provides a fall webworm control dual-trapping and parasitic wasp releasing multifunctional device which comprises a funnel, a groove is formed in the side wall of the funnel and concaves inwards, a plurality of evenly-distributed leakage holes are formed in the side wall of the groove, and a mounting plate is fixedly mounted on the peripheral side face of the funnel. A collecting barrel is movably mounted on the lower side of the mounting plate, the funnel is communicated with the interior of the collecting barrel, and a small bee cocoon placer is movably mounted on the lower side of the collecting barrel; the device is upgraded on the basis of an original fall webworm square trap, a novel Chouioia cunea cocoon placing device is specifically transformed on the lower half part, external wind and rain invasion is blocked, damage to Chouioia cunea cocoons is relieved, the bee output rate of Chouioia cunea is increased, operation is simpler and more convenient, and the device is suitable for popularization and application. According to the upper half part, a square-barrel-shaped trap is modified, a solar LED lamp and sex pheromone are combined for trapping, cost is reduced, and larvae, pupae and adults of the white moths can be prevented and controlled in all stages.

The invention provides an ecological breeding device for mosquito breeding, and relates to the field of biological breeding for biological quarantine. A breeding water tank is fixedly connected to the right part of the top end surface of the fixed bottom plate; a larva grid box is arranged in the breeding water tank; the top end surface of the larva grid box is fixedly connected with a first telescopic box; the top end surface of the first telescopic box is fixedly connected with an eclosion box; a first mosquito-lured lamp is arranged in the eclosion box; the left part of the top end surface of the fixed bottom plate is fixedly connected with a fixed base; the top end face of the fixed base is fixedly connected with a feeding box. A connecting frame is pulled, an insect separation baffle is closed through a series of transmission, an adult box is separated from a feeding box, after the insect separation baffle is closed, a cleaning brush can be driven to move along with continuous movement of the connecting frame to sweep fallen dead mosquitoes, and the problems that space in the adult box is generally compact and narrow, and the adult box is inconvenient to clean are solved. And an experimenter is inconvenient to clean the mosquito corpses in the corners.

The invention discloses an application of a volatile substance in prevention and control of agricultural insect chilo suppressalis, and provides an application of (E)-2-hexenal (trans-2-hexenal) in prevention and control of chilo suppressalis for the first time, so that chilo suppressalis adults can be effectively repelled, the egg laying amount of chilo suppressalis female insects can be inhibited, and the prevention and control of chilo suppressalis can be realized. The natural enemy insects attracting the chilo suppressalis comprise egg parasitic wasp trichogramma japonicum and larva parasitic wasp cotesia chilo suppressalis. The (E)-2-hexenal can reduce the egg quantity of chilo suppressalis in the field, reduce the occurrence cardinal number of the chilo suppressalis, play a prevention and control role and reduce loss before pests cause harm, and meanwhile, the (E)-2-hexenal can attract natural enemy insects of the chilo suppressalis, so that natural enemies in the natural environment can be fully utilized and protected, and the economic benefit of the chilo suppressalis is improved. The improvement of the chilo suppressalis prevention and control effect and the green prevention and control are realized. (E)-2-hexenal is a component in rice volatile matters, and compared with chemical pesticides, (E)-2-hexenal is more beneficial to the ecological environment.

The utility model discloses a mosquito larva feeding and observing device, and relates to the technical field of mosquito larva feeding and observing devices. The device comprises a feeding box, a cone is movably clamped to the top of the feeding box, a locking mechanism used for limiting the cone is arranged on the surface of the feeding box, a flying hole is formed in the top end of the cone in a penetrating mode, and a net cover is connected to the surface of the cone in a sleeved mode. A butt joint assembly used for limiting the mesh enclosure is arranged on the surface of the cone, a positioning ring is movably connected to the bottom of the feeding box in a clamped mode, a supporting plate is arranged below the positioning ring, and a height adjusting assembly used for controlling the positioning ring to move up and down is arranged on the surface of the fixing frame. The device is simple in structure and convenient to use, the effect of conveniently hatching and observing mosquito larvae is achieved in the using process, water and adult mosquitoes can be effectively separated, the situation that the adult mosquitoes die is avoided, in addition, disassembly and assembly are more convenient, feed adding is more convenient, and the situations of splashing and adult mosquito escaping are avoided.

An insect larvae breeding system comprises: a breeding chamber (container 11); a plurality of multistage-type larvae breeding shelves 14 that are arranged in the breeding chamber with each having a plurality of storage racks; a larvae breeding tray T that can be stored in each of the plurality of storage racks and includes a larvae escape prevention member; a movement unit 12 that has a raising / lowering platform and can move along a passage 112 to the side of the plurality of multistage-type larvae breeding shelves 14 and multistage-type loading / unloading shelves 13; and a transfer mechanism that is provided on the raising / lowering platform and transfers the larvae breeding tray T between the raising / lowering platform and storage racks.

The artificial feeding device comprises a breeding mechanism and a humidifying mechanism, the breeding mechanism comprises a breeding container, a water seepage hole is formed in the center of the bottom of the breeding container, a filter screen is arranged at the bottom of the breeding container, and a sandy soil layer is arranged on the upper side of the filter screen; the humidifying mechanism comprises a humidifying container, the lower portion of the breeding container is placed in the humidifying container, and a plurality of water is added to the inner bottom of the humidifying container. The artificial feeding device for the pteropsidae larvae is used for artificial feeding of the pteropsidae larvae, staged isolated breeding can be conveniently carried out according to the growth cycle of the pteropsidae larvae, the breeding environment can be controlled, sandy soil can be conveniently replaced in good time, the insect growth environment is protected, and the artificial feeding survival rate of the pteropsidae larvae is increased; the whole growth process of the larvae is conveniently observed, and the species of the larvae are accurately determined.

The utility model belongs to the technical field of mosquito breeding, and discloses an automatic adult mosquito collecting and breeding box which comprises a larva breeding box, a one-way channel and an adult mosquito collecting box, the larva breeding box is connected with the adult mosquito collecting box through the one-way channel, the larva breeding box comprises a breeding frame, a plurality of layers of breeding trays are placed on the breeding frame, and the breeding trays are arranged on the breeding frame. An over-limit water guide opening is formed in the feeding plate, a drainage groove is formed in the position, corresponding to the over-limit water guide opening, of the feeding frame, the larva feeding box can automatically supply water and feed, the feeding environment is guaranteed, larva feeding is facilitated, the feeding plate can be completely pulled out, cleaning is facilitated, and the larva feeding box can be used conveniently; the funnel-shaped net cylinder in the outer-layer net cylinder can enable adult mosquitoes to fly into the adult mosquito collecting box in a one-way mode, a shading plate is arranged, and the adult mosquitoes are promoted to rapidly leave the larva feeding box and enter the adult mosquito collecting box through the phototaxis of the adult mosquitoes.

The invention belongs to the technical field of artificial cultivation of cordyceps sinensis, and particularly relates to a method for rapidly propagating and culturing cordyceps sinensis based on cordyceps sinensis infection bodies, which comprises the following steps: (1) inoculating cordyceps sinensis into a cordyceps sinensis culture solution for culturing to obtain a cordyceps sinensis infection body culture solution; the cordyceps sinensis is a cordyceps sinensis thallus separated from infected hepialus armoricanus larva or a cordyceps sinensis solid culture which has produced conidia; and (2) mixing the winter worm summer fungus infection body culture solution with the hepialus armoricanus larva feed, feeding the hepialus armoricanus larvae, and carrying out grass emergence culture after the hepialus armoricanus larvae are fed to be rigid. According to the method, the rapid propagation culture infection body is high in concentration (can reach 3.3 * 10 < 7 > / mL), short in period (about 40 days) and high in infection rate (not lower than 80%), a large number of cordyceps sinensis infection bodies can be obtained in a short time, the cordyceps sinensis infection bodies are used for infecting cordyceps sinensis hosts to obtain cordyceps sinensis, and the problems that the source of artificially matched and planted cordyceps sinensis strains is unstable, and strain preparations are difficult to store for a long time can be solved.

The embodiment of the invention discloses a feeding method for improving the survival rate and the weight of protaetia brevitarsis larvae in the process of treating and converting mushroom residues of the protaetia brevitarsis larvae. The breeding method comprises the following steps: S1, obtaining excrement generated in the breeding process of 2-age and / or 3-age protaetia brevitarsis larvae; s2, acquiring an excrement matrix formed by the excrement; s3, 2-year-old protaetia brevitarsis larvae are put into the excrement substrate and put in the environment with the temperature being 28 + / -2 DEG C and the illumination period being L: D = 16h: 8h to be bred for 10 days, and the humidity of the substrate is controlled to be 50%-55%; and S4, picking out or screening out the larvae fed for 10 days from the excrement substrate, and putting the larvae into the mushroom bran substrate for continuous feeding. According to the method, the survival rate and weight of the larvae in the process of treating and converting the protaetia brevitarsis larvae into the mushroom bran can be increased, the utilization rate of fecula is increased, the pretreatment process of the mushroom bran is simplified, the feeding cost is reduced, the feeding method is simple and feasible, materials are convenient to take, and the industry of treating and converting the protaetia brevitarsis into the mushroom bran is greatly promoted.

The invention relates to application of 8-Br-cGMP in induction of scallop attachment and metamorphosis, and belongs to the technical field of aquaculture, and the application method is as follows: when the number of eyespot larvae of scallops reaches more than 50%, the 8-Br-cGMP acts on a scallop larva hatching pond according to the concentration of 1 * 10 <-5 >-1 * 10 <-7 > M. When 8-Br-cGMP is added into a culture water body, the attachment metamorphosis rate of scallops can be effectively increased, and when 0.01 mM of 8-Br-cGMP is added, the metamorphosis rate of scallop larvae can reach 93.3%.

The invention discloses application of cordyceps javanica Bd01 and a dispersible oil suspending agent thereof to prevention and control of spodoptera frugiperda, and relates to the technical field of biology. The research finds that the cordyceps javanica Bd01 has a strong infection lethal effect on larvae of spodoptera frugiperda at all ages, the pupation rate and eclosion rate of the larvae can be remarkably reduced, and the breeding cycle of pests can be effectively blocked. The invention further develops a dispersible oil suspending agent, and the suspending rate is effectively improved through formula optimization. The dosage form takes soybean oil as a dispersion medium, does not contain harmful solvents such as methylbenzene, is free of dust pollution and is friendly to operators and the environment, and ascorbic acid is added to improve the ultraviolet resistance of spores and prolong the effective period of the pesticide effect. In addition, the preparation also has a certain control effect on caffeinidae, is wide in application scene, provides a new scheme for green control of spodoptera frugiperda, solves the problems of chemical pesticide resistance and residue, and accords with the development requirements of green agriculture.

The artificial breeding and island plant pollination method comprises the following steps: artificial breeding of aphids: broad bean seedlings are planted in an insect breeding cage to propagate black bean aphids, so that adults of the hoverflies can lay fly eggs, and larvae of the hoverflies can eat the fly eggs at the same time; feeding of hoverfly larvae: feeding the hoverfly larvae with black bean aphids in an insect breeding cage, and enabling the hoverfly larvae to develop into pupae after 6 days; collecting pupae of hoverflies with short thorn legs: harvesting broad bean seedlings with mature larvae of hoverflies, putting the broad bean seedlings into a plastic box with a gauze opening for continuous feeding, and putting the collected pupae of hoverflies into a culture dish until all the larvae in the plastic box pupate; and transporting and releasing the short-spur hoverflies, namely storing pupae of the hoverflies in a pupa card device at the temperature of 4 DEG C, transporting the pupae to an open sea island, and releasing the pupae. By the adoption of the method, scale breeding can be conducted on the species of the hoverfly brachypoda, and the hoverfly brachypoda can be transported to the island area and used for pollination of island plants.

The utility model discloses an intelligent cultivation type insect development progress monitor which comprises an incubator cultivation system, a water supply system and a development monitoring system, a right side plate of an incubator is provided with an oppositely-opened box door, and other side plates of the incubator are fixedly connected with shutters; the cultivation system is arranged on the inner wall of the left side of the cultivation box and used for cultivating overwintering larvae; the water supply system is used for supplying water to the cultivation system; the overwintering larva breeding system has the advantages that the collected overwintering larvae are bred in the breeding system, water is supplied to the breeding system through the water supply system in the breeding process, and the breeding system is connected with the breeding system through the water supply system. The water supply system supplies water required by pupation and eclosion after overwintering of larvae, the larvae grow and develop in the breeding system, the development condition of the larvae is monitored through the development monitoring system so that the development progress of the overwintering larvae can be known in time, and guidance is provided for prevention and control of pests.

The invention belongs to the field of microorganisms, and particularly discloses Aspergillus nomiae for preventing and controlling spodoptera frugiperda. The technical problem to be solved by the invention is how to prevent noctuid insects from harming plants. The invention provides aspergillus for preventing and treating noctuid insects from harming plants, the aspergillus is Aspergillus nomiae WNAN1, and the preservation number of the aspergillus nomiae is CGMCC (China General Microbiological Culture Collection Center) No.41741. The invention further provides a preparation method of the aspergillus nomiae. Experiments prove that the aspergillus has a relatively high fatality rate on third-instar larvae of the spodoptera frugiperda, and can be used for preventing and controlling the spodoptera frugiperda.

The invention relates to a compact, low-profile aquatic trap designed to be directly submerged in mosquito larval habitats, aimed at capturing and retaining them underwater long enough to kill them by lack of air. The entrance of the trap includes multiple niche-like openings to trap the larvae by offering them shelter from light and predators. These entrances lead to a series of compartments that form a labyrinth guiding the larvae towards an inescapable chamber. Being anaerobic, the trap causes the larvae to die in the inescapable chamber within a few hours, without bait, chemicals, energy, or any kind of refills.

To solve problems for example, since flying insects fluttering freely, so flying insects may flutter into an external space when opening a lid or a door carelessly, so that, feeding flying insects has to be performed carefully.SOLUTION: A device for breeding insects and larvae of insects has: a breeding container for partitioning an internal space in which insects are bred and an external space; a feeding hole provided on a part of the breeding container; and a feeding unit provided to contact the feeding hole from the outside of the breeding container. The breeding container has a feeding part protruded to the internal space, and the feeding hole may be provided on the feeding part. The breeding container has the feeding part protruded outward from the internal space, and the feeding hole may be provided on the feeding part. The breeding container has a floor surface, wall surfaces, and a ceiling surface, and the feeding part is provided on any one of the floor surface, the wall surfaces and the ceiling surface. For example, the breeding container has at least the floor surface, and the feeding hole may be provided on the floor surface.SELECTED DRAWING: Figure 1

The invention discloses an RNA (Ribonucleic Acid) interference method for parasitic wasps in lepidoptera larvae, which is characterized in that RNA interference is carried out by using a microinjection method or a nano-material immersion method when the parasitic wasps in the lepidoptera larvae are not separated from hosts or are separated from the hosts to cocoon, so that an RNAi system of the parasitic wasps is successfully constructed, and the defects that the parasitic wasps are small in size in an egg stage, and the parasitic wasps are not damaged in the egg stage can be effectively overcome. And RNAi operation difficulty caused by cocoon wrapping in the pupal stage and short life in the adult stage is avoided. Moreover, the method disclosed by the invention is simple to operate and high in interference efficiency, provides a theoretical basis and a reference effect for further researching gene functions of the parasitic wasps, and has important significance on biological control.

The invention relates to the technical field of biological identification, in particular to a method for quantifying the quality of wasp larvae and bee pupae, and mainly aims to solve the problem that the artificial wasp breeding industry lacks objective quality judgment standards. The method comprises the following steps: freezing a wasp honeycomb so as to conveniently take down larvae and bee pupae, weighing, drying, grinding and the like on a sample, measuring the content of aspartic acid and glutamic acid by utilizing an amino acid analyzer, performing comprehensive calculation by combining indexes such as individual weight and the like, and finally determining the content of aspartic acid and glutamic acid according to a plurality of character values. The quality is judged according to the average content and the total weight of glutamic acid and aspartic acid and the excrement residue condition. The method can effectively distinguish quality differences of wasps of different insect states and species, provides a fair basis for market transactions, promotes sustainable development of the wasp breeding industry, and the effectiveness of the method is verified by specific embodiments.