Artificial diet for Lepidopterous young larvae, preparation method for artificial diet and application of artificial diet

Abstract

The invention provides an artificial diet for Lepidopterous young larvae. The artificial diet for the Lepidopterous young larvae is prepared from the following raw materials in percentage by weight: 2.0 to 3.0 percent of soybean cake meal, 7.0 to 10.0 percent of corn flour, 2.0 to 3.0 percent of Saccharomyces cerevisiae Hansen, 7.0 to 10.0 percent of tomato sauce, 0.8 to 1.2 percent of sucrose, 1.2 to 1.4 percent of agar, 0.2 to 0.5 percent of sorbic acid, 0.05 to 0.1 percent of vitamin B complex, 0.2 to 0.4 percent of ascorbic acid, 0.1 to 0.2 percent of ethyl p-hydroxybenzoate, 0.2 to 0.4 percent of propionic acid and 71.0 to 77.0 percent of water. The invention also provides a method for preparing the artificial diet and application of the artificial diet. The artificial diet is low in manufacturing cost, easy to operate and high in practicability; and by the artificial diet and the raising method for raising the Lepidopterous young larvae, the survival rate of the larvae is high, the workload is low, and manpower and material resources are greatly saved.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to an artificial feed for young larvae, a preparation method and application thereof. Background technique [0002] Feeding insects with artificial feed is one of the basic techniques for studying entomology. This method is not limited by seasons, and the feeding and management are convenient. It is easy to obtain a large number of test insects with the same physiological indicators and directly carry out insect biology, genetics, and resistance testing. etc. (Wu Kunjun et al., 2009; Jiang Jinwei et al., 2010). Since the 1950s, the research on artificial feed at home and abroad has been paid more and more attention and developed rapidly. According to incomplete statistics, so far, more than 1,000 species of insects at home and abroad can be reproduced using artificial feed, which provides valuable data for studying the biology of different insects and guiding agricultura...

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Problems solved by technology

The research on the life table of laboratory populations also basically uses the "instar group" as the insect stage, which refers to the division of 1-2 instar larvae, 3-6 instar larvae, pupae, adults, eggs, etc., and different instars cannot be obtained for the larval stage. The developmental duration and survival rate of instar larvae and other important data cannot be carried out related research work such as bioassay of different instar larvae

In addition, the group rearing of young larvae still has the following problems: (1) Due to the high density of insects, once infected with viruses or bacteria, it is easy to infect other insects, which will have a devastating impact on the experiment

(2) During the experiment, some young larvae have the habit of boring and often bore into the feed, and need to constantly turn the feed to find the larvae, which is not only a waste of time, but also can cause artificial harm to the experimental insects and affect the experimental results

(3) Insect larvae ages often overlap. Multiple insects are reared in groups, and it is impossible to prepare and record the development status of each insect. It is quite troublesome to calculate the developmental duration and mortality of young insects. In order to make the data more scientific , the number of sampling must be increased, which consumes a lot of manpower and material resources

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