105 results about "Riboflavin" patented technology

The invention belongs to the technical field of dressing preparation, and particularly relates to a preparation method of a photocuring methacrylated gelatin hydrogel dressing, which comprises the following steps: step 1, dissolving methacrylated gelatin in a phosphate buffer solution to prepare a methacrylated gelatin solution with the mass concentration of 5-15%; 2, adding a natural photoinitiator into the solution obtained in the step 1, and uniformly stirring and mixing; 3, adding the traditional Chinese medicine active ingredient powder, and stirring until the powder is uniformly dispersed to obtain a mixed solution; and 4, pouring the mixed solution into a mold, and curing and molding under the irradiation of visible light. Chlorophyll or riboflavin is used as a natural photoinitiator and is directly dispersed in a methacrylated gelatin solution in a physical blending mode, a curing light source enables chlorophyll to completely avoid a chemical initiator, meanwhile, the process is simplified into physical stirring to replace nanoparticle synthesis and modification, visible light reduces the risk of tissue burn, the production cost is reduced, and the method is suitable for industrial production. The method is suitable for primary medicine.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention provides application of riboflavin in inhibition of postharvest yellowing of broccoli, discovers that yellowing and aging of broccoli can be effectively inhibited by soaking broccoli with a riboflavin-containing solution, and the mechanism of riboflavin in inhibition of broccoli yellowing is deeply studied. The riboflavin is found to be capable of inhibiting expression of chlorophyll degradation genes and chlorophyll aging genes in the broccoli and delaying synthesis of carotenoids by the broccoli, so that yellowing and aging of the broccoli are effectively inhibited. The broccoli treated by the method disclosed by the invention has an excellent treatment effect, and yellowing and aging of the broccoli can be obviously delayed and the shelf life can be prolonged when the broccoli is stored at normal temperature, so that the method has great significance in storage of the broccoli and is also greatly helpful for production and sales of the broccoli.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention belongs to the field of environmental biotechnology and nano material application, and relates to a method for accelerating microorganisms to reduce selenite based on immobilized riboflavin. Aiming at the problems of poor stability, difficulty in recovery, non-repeated use and the like of dissolved riboflavin serving as an electron shuttle, riboflavin is grafted on the surface of a multi-walled carbon nanotube through a covalent binding method, and immobilized riboflavin capable of being recycled is constructed. The material can accelerate extracellular electron transfer of shewanella MR-1, and toxic sodium selenite is efficiently reduced into low-toxicity or non-toxic elemental selenium. Results show that the immobilized riboflavin not only maintains high reduction activity of riboflavin, but also has excellent cyclic utilization performance, still maintains high reduction rate after being repeated for multiple times, and effectively reduces operation cost and resource loss. According to the invention, an efficient, economical and sustainable application scene is provided for a microorganism-electron shuttle combined remediation technology, and a new thought is also provided for application of a functional nano material in an environmental biotechnology.

The invention discloses a prefabricated photocatalyst film for retaining freshness of leaf vegetables as well as a preparation method and application of the prefabricated photocatalyst film. The photocatalyst film comprises the following components in parts by weight: 100 parts of chitosan; 0.5 to 2 parts of metal-nitrogen doped titanium dioxide; 0.5 to 1 part of riboflavin; 30 to 40 parts of glycerol; wherein the metal-nitrogen doped titanium dioxide is iron-nitrogen doped titanium dioxide and / or copper-nitrogen doped titanium dioxide. The photocatalyst film is used for sealing the prefabricated leaf vegetables, composite LED illumination in the cold chain distribution process is combined, the antibacterial and fresh-keeping effects are achieved, microbial infection of the prefabricated vegetables in the cold chain distribution process can be reduced, water loss and chlorophyll degradation can be reduced, and the shelf life of the prefabricated vegetables can be prolonged.

The invention discloses a degradable plastic quality release and demolding residue traceability management method, and relates to the technical field of quality release traceability, and the method comprises the following steps: 1, doping a fluorescent whitening agent European in a polylactic acid matrix to form an inner channel, attaching a polyvinyl alcohol witness sheet to the edge of a cavity, and presetting riboflavin to form an outer channel, establishing a dual-channel ultraviolet detection position; 2, collecting two paths of intensity, calculating a ratio coefficient, and establishing mapping with surface residues to form red, yellow and green threshold values and a correction list; 3, a workpiece is judged through a black box, spray volume, wind shear and mold temperature instructions are automatically given, a witness piece is separated from warm water and confirmed through a background, and meanwhile a cavity-level demolding event is generated and bound with the digital link two-dimensional bar code; and step four, third-party compliance files are archived in a ticket-folder manner with quantiles as reference to a reconvergence threshold value, so that mold-crossing and line-crossing consistent release and verifiable traceability are realized, and food contact and compostable boundaries are considered. And the release efficiency and stability are improved.

The invention relates to a livestock breeding technology, and discloses a preparation method of a premix for enhancing laying hen production treatment, in the construction of a basic nutrition framework, the construction of a vitamin system is a core project, vitamin A is supplemented by adopting a palmitate form, the stability of the vitamin A in feed processing is ensured, the level of 8000-12000IU should be maintained in each kilogram of feed, and the content of the vitamin A in the feed processing is reduced. According to the fallopian tube mucous membrane composition, the addition amount of the vitamin D3 in the metabolic activity form of 25-hydroxyD3 needs to reach 2000-3000 IU / kg, the vitamin D3 and parathyroid hormone have a synergistic effect, the calcium and phosphorus deposition efficiency can be remarkably improved, particularly, the synergistic effect of B vitamins is worthy of attention, and thiamine (B1) and riboflavin (B2) are compatible according to the proportion of 1: 2. According to the preparation method of the premix for enhancing laying hen production treatment, the growth speed and the laying efficiency of chicken flocks are effectively improved, the eggshell quality is ensured, nutritional diseases such as chondropathy and egg reduction diseases are reduced, and the economic benefits of laying hen breeding are greatly improved.

An instant fish maw processing technology for delaying degradation of collagen belongs to the technical field of instant fish maw processing, and comprises the following steps: S1, treating fish maw with 0.1% of tea polyphenol, 0.05% of a rosemary extract and 0.2% of chitosan oligosaccharide for 30 min, carrying out enzyme inhibition treatment, and then cleaning and draining for later use; s2, dipping the fish gelatin treated in the step S1 in a solution containing 0.1% of riboflavin and 0.2% of hyaluronic acid, irradiating the fish gelatin with white light for 10s, and then cleaning and draining the fish gelatin for later use; and S3, putting 5-7 parts by weight of fish maw and 65-80 parts by weight of pure milk into a container, sealing, and sterilizing to obtain the instant fish maw. Through enzyme inhibition treatment, the hydrolysis capability of metalloproteinase on collagen is reduced or eliminated, and degradation of the collagen is reduced, so that the nutritional value of the fish maw is maintained; collagen molecules in the fish maw and riboflavin are subjected to a cross-linking reaction to form a stable three-dimensional network structure, so that the acting force between the collagen molecules is enhanced, the structural stability of the collagen is improved, and the degradation of the collagen is delayed.

This invention relates to the field of functional food technology, and more particularly to the application of safflower honey, or safflower honey extract, in enhancing the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). The invention has found that safflower honey, or safflower honey extract, can effectively enhance ADH and ALDH activity, thereby promoting alcohol metabolism and achieving a hangover-relieving effect. Further research on the extract revealed that a mixture of syringaldehyde, riboflavin, lutein, luciferin, and three p-coumaroyl spermidine plays a key role in enhancing ADH and ALDH activity, thus providing an application of this mixture in hangover relief. The application of safflower honey and its extract provided by this invention has high application value for developing foods and health products with hangover-relieving effects.

The invention discloses an escherichia coli strain with high yield of riboflavin as well as a construction method and application of the escherichia coli strain. A series of Escherichia coli strains with high yield of riboflavin are constructed, the constructed Escherichia coli with high yield of riboflavin is safe and harmless, glucose is used as a substrate for fermentation under the aerobic condition of a shake flask, the yield of riboflavin is 1117.8 mg / L, 1330.1 mg / L, 1430.8 mg / L, 1508.7 mg / L and 1640.8 mg / L in sequence, and the yields are 112.5 mg / g of glucose, 134.1 mg / g of glucose, 146.3 mg / g of glucose, 158.8 mg / g of glucose and 168.1 mg / g of glucose in sequence; when the strain SN04M is subjected to fed-batch fermentation in a 1L fermentation tank, the yield of riboflavin reaches 8.52 g / L, and the total yield is 189.9 mg / g glucose. According to the invention, the fermentation yield of riboflavin and the substrate utilization rate of riboflavin to glucose can be obviously improved.

The present invention relates to a fluorescent inspection liquid which is highly safe to a human body and emits blue fluorescence having high visibility in the leakage inspection. The fluorescent inspection liquid contains water as a solvent and two blue fluorescent dyes selected from a flavin derivative, quinine, pyrene and anthocyanin. The flavin derivative is a derivative having a flavin skeleton and obtained from a starting material, riboflavin.

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.

The invention belongs to the technical field of solid beverages, and particularly discloses a functional solid beverage and a preparation method thereof.The solid beverage is composed of superfine mixed powder formed by conducting low-temperature baking, wall breaking treatment and screening on components in percentage by mass: 15% of sea-buckthorn, 15% of white mulberry, 12% of dandelion, 12% of radix polygonati officinalis, 12% of rhizoma polygonati, 30% of black fruit small molecule peptide, 1% of riboflavin and 3% of monosaccharide. The low-temperature baking is a treatment process in which each component is independently performed under the condition of 40-60 DEG C and the duration time is longer than 24 hours, and a synergistic technical scheme of differential pretreatment and accurate low-temperature baking effectively solves the technical problem that active components are degraded due to high-temperature and non-protection treatment in traditional solid beverage processing; in the raw material pretreatment stage, plant-derived raw materials such as hippophae rhamnoides and white mulberries are pre-dried at the temperature of 50-60 DEG C for humidity control, and polygonatum kingianum is steamed for 2-3 hours to convert irritant components.

The invention belongs to the technical field of vitamin B2 production, and particularly relates to a preparation method of a safe high-yield strain for vitamin B2 production, which comprises the following steps: S1, strain source and screening: analyzing the current situation and development trend of vitamin B2, and screening the strain; s2, genetic engineering modification: evaluating the production performance and genetic stability of the strain, and carrying out mutagenesis treatment on the screened strain by using a chemical mutagenic agent to obtain a genetically mutated strain; s3, screening and identifying high-yield mutant strains: integrating the fragments containing the spore cereus dry riboflavin operon into a vitamin B2 genome to generate the mutant strains, and performing fine adjustment on the riboflavin operon in the mutant strains by utilizing a multiple genome editing system. And through technical popularization, the manufacturing cost of the product can be reduced, and the social benefit is obvious.

The present invention is related to an improved process for production of vitamin B2 using a genetically engineered host cell comprising a heterologous enzyme with pyridoxal phosphatase activity. Using said modified host cell, the yield of riboflavin production could be increased by at least about 5%.

This application relates to the field of water pollution control technology and discloses a method for preparing a sediment microecological reconstruction and pollutant-targeted immobilization conditioner. The method includes: modifying mesoporous silica with amino- and thiolated compounds and mixing it with biochar to obtain an adsorbent material; dispersing ferrous sulfate, sodium thiosulfate, and hydroxyapatite with the adsorbent material in a sodium alginate solution and adding it dropwise to a calcium chloride solution for cross-linking and chitosan coating to obtain microcapsules; compounding riboflavin, humic acid, carbon source nutrients, and adding signaling molecules to obtain a metabolic enhancer; compounding metalloenzyme inhibitors and activating them with microorganisms to obtain an activator; granulating and coating calcium carbonate, magnesium hydroxide, ferrous sulfate, sulfur powder, calcium peroxide, and sodium lactate to obtain buffer particles; mixing the adsorbent material, microcapsules, and buffer particles, spraying, adding the metabolic enhancer and activator, adding binders and pore-forming agents, granulating, drying, sieving, and spraying a protective film to obtain the conditioner. This application solves the technical problems of existing sediment conditioners, such as single function, uncontrolled release, and microecological imbalance.

Methods and systems for reducing acrylamide concentration in heat-processed products, and products produced by such methods and systems are provided. The baked products may be sprayed with a riboflavin solution and then irradiated with a UV light source to initiate monomer reactions of acrylamide and reduce the concentration of the acrylamide in the baked product. In addition, riboflavin may be dissolved in heat-processed products, followed by irradiation of the riboflavin-containing heat-processed products with a UV light source to initiate monomer reactions of acrylamide and reduce the concentration of the acrylamide in the baked product.

The disclosure provides methods and apparatuses for removing a yellow color of riboflavin in a lactose solution. The methods include exposing a lactose solution (e.g. milk) containing riboflavin to light under conditions that breakdown riboflavin comprised within the solution to thereby remove the yellow color of riboflavin. The conditions that breakdown include a specific wavelength of light (e.g. light having a wavelength of 360-475 nm) and a specific temperature range (e.g. a temperature of 0-80° C.). In certain embodiments, the duration of the treatment is such that the lactose solution is exposed to a total of 5-20 kW of light.

The present invention to the identification of a novel isolated bacterial strain, the Limosilactobacillus reuteri strain which is characterized by a naturally occurring overproduction of riboflavin (vitamin B2) compared to other known strains of lactobacilli. Said strain of the Limosilactobacillus species is deposited under accession number LMG P-32020. In a further aspect, also the uses of said L. reuteri strain are disclosed herein. In particular, the use of said strain in food and feed industry, human and veterinary health, large-scale vitamin production, cosmetics and consumer care products is disclosed.

The invention discloses a method for preparing food-grade Janus particles based on confinement template synergistic sequential curing. The method comprises the following steps: firstly, dissolving riboflavin and hydrogen peroxide in a zein solution to serve as a water phase, and dispersing in an oil phase to prepare zein droplets; meanwhile, preparing sodium alginate liquid drops by taking a sodium alginate solution as a water phase; and mixing the two liquid drops to construct the Janus liquid drop based on a liquid drop collision method. Subsequently, the zein compartment in the Janus droplet is cured by ultraviolet irradiation; and further mixing the sodium alginate with calcium chloride liquid drops to solidify the sodium alginate compartment in the Janus liquid drops to obtain the food-grade Janus particles. The particle size of Janus liquid drops is reduced to nanoscale through gradient energy input, and food-grade Janus nanoparticles are constructed by using a sequential curing strategy. The construction method of the food-grade Janus particles is easy to operate and high in yield, and has good application prospects in the fields of food, medicine, cosmetics and the like.

This invention relates to the field of electrospun membranes, and more specifically to a one-step photocurable electrospun membrane material and its preparation method. The photocurable electrospun membrane material is prepared by mixing 5-20 parts of fiber-forming macromolecules, 10-40 parts of collagen, 0.05-3 parts of natural photosensitizer, and a solvent to form a spinning solution. Electrospinning is performed using either needle-type or non-needle-type methods. Simultaneously, the fibers are irradiated with 200-400 nm ultraviolet light in situ to achieve photocuring and cross-linking, thus obtaining a micro / nanofiber membrane in one step. The fiber-forming macromolecules are selected from biodegradable medical polymers, and the photosensitizers are biosafe components such as riboflavin and retinaldehyde. This invention completely solves the problems of uneven cross-linking, fiber morphology damage, collagen denaturation, and internal light filtering effects in traditional two-step methods. The resulting membrane has regular fiber morphology, uniform cross-linking, high mechanical strength, good water resistance, and complete retention of bioactivity. Furthermore, the process is simplified, complies with pharmaceutical quality management standards, and is suitable for medical applications such as tissue engineering and wound repair.

The invention discloses a preparation method and application of novel biomass carbon dots. The preparation method comprises the following steps: S1, adding apple branch powder into water, and uniformly mixing to obtain a mixed solution; and S2, putting the mixed solution obtained in S1 into a microwave oven for microwave treatment to obtain a reactant. S3, taking out reactants, cooling the reactants to room temperature, and adding water for centrifugal filtration treatment. And S4, collecting a supernatant obtained by treatment in S3, and purifying the supernatant. And S5, drying the purified product obtained in S4 to obtain the biomass carbon dots, and storing the prepared biomass carbon dots in an environment with a constant temperature of 4 DEG C for later use. Apple branches are used as precursors, the biomass carbon dots are synthesized through a microwave method, biocompatibility is good, toxicity is low, high-selectivity and high-sensitivity detection of Cr < 6 + > and riboflavin can be achieved, the synthesis process is economical, environmentally friendly, rapid, simple and convenient, and a new scheme is provided for the environment-friendly detection technology.

The utility model relates to a kind of oxygen carrier uniform distribution stirring devices of riboflavin fermentation tank in the field of fermentation equipment, including stirring paddle, and stirring paddle includes stirring shaft and the stirring blade fixed on stirring shaft;Gas passage one is equipped in stirring shaft, and the top of gas passage one is connected with air inlet pipe by swivel joint, and stirring blade is equipped with gas passage two, and gas passage two is communicated with gas passage one, and the outlet hole one is equipped on stirring blade, and the outlet hole one is communicated with gas passage two, and stirring blade includes multiple stirring rod one and multiple stirring rod two, and the length of stirring rod one is less than the length of stirring two, and the outlet hole one is located at the end of stirring rod one and stirring rod two.This utility model passes into oxygen to the different position of fermentation tank and stirs the material in fermentation tank, improves the mixing efficiency and mixing uniformity of material and oxygen;The position of gas guide pipe in gas passage one can be adjusted, so that more reasonable oxygen is passed into fermentation tank, which is beneficial to fermentation.

The invention relates to the field of environmental restoration, in particular to a riboflavin-mediated plant aluminum toxicity prevention and control method and application thereof, and the riboflavin-mediated plant aluminum toxicity prevention and control method comprises a precursor of flavin mononucleotide and flavin adenine dinucleotide consisting of isoxazine ring and ribitol, and an oxidation-reduction reaction participated in the forms of FMN and FAD. Compared with the prior art, the invention has the advantages; according to the method, water-soluble vitamin B2 riboflavin is adopted, riboflavin with the concentration of 100 [mu] M is added into the aluminum-polluted water body, the water-soluble vitamin B2 riboflavin is applied to a duckweed growth system, and duckweed thallus breeding and root system growth can be remarkably promoted; riboflavin with strong adsorption capacity to aluminum ions can effectively reduce the aluminum content in water and plants and reduce active oxygen generated by the plants in an aluminum stress environment so as to relieve the damage of aluminum toxicity to the plants, and riboflavin shows a remarkable effect on relieving aluminum toxicity, can provide a novel solution for improvement of stress resistance of crops, and has a broad application prospect. And the method has a wide application prospect in polluted environment restoration.

The invention discloses an acetic acid inhibition culture medium and application thereof in lactic acid fermentation production. The acetic acid inhibition culture medium is prepared from 15-30 g / L of peptone, 5-10 g / L of yeast extract powder, 50-100 g / L of glucose, 1-2 mL / L of Tween, 1-2 g / L of ammonium citrate, 1-2 g / L of dipotassium phosphate, 0.2-0.4 g / L of magnesium sulfate, 0.1-0.2 g / L of ferrous sulfate, 0.1-0.2 g / L of copper sulfate, 0.05-0.2 g / L of manganese sulfate, 0.005-0.02 g / L of biotin and 0.005-0.02 g / L of riboflavin. The method has remarkable technical advantages in the aspects of inhibiting the generation of a byproduct acetic acid and improving the yield of lactic acid and the fermentation stability, can effectively overcome the defects in the prior art, and has relatively high economic value and popularization and application prospects.

This invention belongs to the field of bioengineering technology and discloses a strain of *Pseudomonas putida*. purF Gene knockout strains and their applications are disclosed in detail. purF Gene reagents or inhibitors purF The application of gene expression reagents in enhancing the ability of *Pseudomonas putida* to synthesize riboflavin. This invention discloses for the first time the method of enhancing the ability of *Pseudomonas putida* to synthesize riboflavin by knocking out or inhibiting gene expression in *Pseudomonas putida*. purF Genes or their expression can significantly enhance the iron deficiency adaptation, adhesion and biofilm formation of *Pseudomonas putida*, and can also regulate the expression of iron balance-related genes in fish hosts, thereby increasing the iron content in fish host tissues.

A device designed to assist in corneal cross-linking procedures. The device comprises a body portion having a top side and a bottom side, the latter characterized by a concave portion designed to be placed on the epithelium of the cornea to achieve a uniform pressure distribution. The device comprises a plurality of 50 micron high epithelium thorns on the bottom side, intended to create microperforations in the corneal epithelium when pressure is applied to the top side of the device. By creating these microperforations, the device helps to improve penetration of therapeutic agents, such as riboflavin, in corneal cross-linking procedures. The device is made of silica gel and is shaped like a contact lens, represents the innovative application of the microneedle technology in the ophthalmic field, and solves the key limitation related to the traditional drug delivery method leading to the eye.

The invention belongs to the technical field of environmental treatment, and particularly relates to a dynamic membrane bioreactor, application thereof and a selenium-containing wastewater treatment method. The dynamic membrane bioreactor provided by the invention comprises a shell, a membrane assembly arranged in the shell and a filler dispersed in the shell, the membrane assembly comprises a porous support carrier and a dynamic biological membrane covering the surface of the porous support carrier; the filler comprises a carbon nanotube and / or immobilized riboflavin, and the immobilized riboflavin comprises an acidified carbon nanotube and riboflavin grafted on the surface of the acidified carbon nanotube. The reactor provided by the invention can slow down the pollution of the membrane module and prolong the efficient and stable operation period of the dynamic membrane bioreactor.