150 results about "Riboflavin" patented technology

The invention provides a fluorescence imaging method and device based on light source proportion adjustment and a storage medium, and the method comprises the steps: in a photodynamic diagnosis PDD fluorescence mode, when a blue light source is turned on, after blue light is reflected by tissues, filtering the spectrum of a green fluorescence wave band generated by excitation of riboflavin through a notch filter, the interference of green fluorescence on PDD fluorescence contrast is reduced; in the photodynamic white light image mode, red light, green light and blue light are controlled to be imaged in a stroboscopic mode through the light compensation module, and the proportion of the light power of the red light, the green light and the blue light is controlled, so that the red light, the green light and the blue light are reflected by observation tissue, penetrate through the notch filter and then are synthesized into white light. By combining the notch filter and the light compensation module, the problem that in the PDD fluorescence imaging process, green fluorescence generated by excitation of riboflavin affects the contrast ratio of PDD fluorescence imaging is solved, and meanwhile color rendition of traditional white light imaging can be guaranteed.

The invention discloses riboflavin photodynamic antibacterial hydrogel for a diabetes wound dressing as well as preparation and application of the riboflavin photodynamic antibacterial hydrogel, and belongs to the technical field of biomedical materials. The hydrogel is prepared from carbomer 941, rhizoma bletillae polysaccharide and carboxymethyl chitosan through physical and chemical bi-crosslinking, glutaraldehyde and carboxymethyl chitosan are added to form dynamic imine bonds, and the stability and adaptability of the hydrogel are enhanced. Meanwhile, the photosensitizer riboflavin is introduced, so that the hydrogel is endowed with photodynamic sterilization characteristics, efficient antibiosis is realized, and rapid healing of diabetic wounds is promoted. The hydrogel has excellent wound environment adaptability and adhesion performance, and is suitable for complicated wound surfaces of diabetes mellitus. The successful research and development of the invention provide reference for the development of novel photodynamic wound dressings, have wide application prospects, provide better treatment choices for diabetics, and are helpful for accelerating wound healing, reducing infection risks and improving the life quality of patients.

The invention belongs to the technical field of dressing preparation, and particularly relates to a preparation method of a photocuring methacrylated gelatin hydrogel dressing, which comprises the following steps: step 1, dissolving methacrylated gelatin in a phosphate buffer solution to prepare a methacrylated gelatin solution with the mass concentration of 5-15%; 2, adding a natural photoinitiator into the solution obtained in the step 1, and uniformly stirring and mixing; 3, adding the traditional Chinese medicine active ingredient powder, and stirring until the powder is uniformly dispersed to obtain a mixed solution; and 4, pouring the mixed solution into a mold, and curing and molding under the irradiation of visible light. Chlorophyll or riboflavin is used as a natural photoinitiator and is directly dispersed in a methacrylated gelatin solution in a physical blending mode, a curing light source enables chlorophyll to completely avoid a chemical initiator, meanwhile, the process is simplified into physical stirring to replace nanoparticle synthesis and modification, visible light reduces the risk of tissue burn, the production cost is reduced, and the method is suitable for industrial production. The method is suitable for primary medicine.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention discloses application of a polymer coupled with a flavin group to catalysis of conversion of NADH (nicotinamide adenine dinucleotide) in a reduction state into NAD + (nicotinamide adenine dinucleotide) in an oxidation state, which is characterized in that in a solution containing various components, the polymer coupled with the flavin group catalyzes the NADH to convert the NAD +, the conversion rate is 0.007-0.85 mu M / s, and the conversion efficiency is 20-100%; the polymer coupled with the flavin group is a product obtained by coupling flavin with a polymer containing a cationic group side group through a covalent bond, the molar ratio of the flavin group in a product molecule to the cationic group in the polymer is 1: 1-1: 100, and the polymer is composed of a repetitive unit containing the cationic group and other repetitive units. Wherein the molar fraction of the amino-containing repeating unit is 5%-100%. Compared with riboflavin, flavin mononucleotide and other biomolecules containing flavin groups, the activity of catalyzing NADH to be oxidized into NAD + can be improved by at least 50 times or above.

The invention provides application of riboflavin in inhibition of postharvest yellowing of broccoli, discovers that yellowing and aging of broccoli can be effectively inhibited by soaking broccoli with a riboflavin-containing solution, and the mechanism of riboflavin in inhibition of broccoli yellowing is deeply studied. The riboflavin is found to be capable of inhibiting expression of chlorophyll degradation genes and chlorophyll aging genes in the broccoli and delaying synthesis of carotenoids by the broccoli, so that yellowing and aging of the broccoli are effectively inhibited. The broccoli treated by the method disclosed by the invention has an excellent treatment effect, and yellowing and aging of the broccoli can be obviously delayed and the shelf life can be prolonged when the broccoli is stored at normal temperature, so that the method has great significance in storage of the broccoli and is also greatly helpful for production and sales of the broccoli.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention discloses a riboflavin soluble microneedle and application of the riboflavin soluble microneedle in photodynamic antibacterial treatment. The invention provides a soluble microneedle patch with an antibacterial function. The soluble microneedle patch comprises a needle body and a backing layer, the needle body comprises a needle body matrix material and a medicine; the medicine is riboflavin and nano-silver; the needle bodies are arranged on the backing layer in an array mode. The microneedle prepared by the invention is good in mechanical strength, can quickly release the medicine, can penetrate through the skin to deliver the medicine to a certain depth, has a photodynamic antibacterial effect, and is suitable for being applied to photodynamic antibacterial treatment.

To provide cancer vaccine compositions and methods of use thereof.SOLUTION: The disclosure provides a cancer vaccine composition comprising inactivated cancer cells and an adjuvant, wherein the inactivated cancer cells are incapable of performing replication. Also there is provided a method for producing a cancer vaccine composition, the method comprising contacting cancer cells with light (e.g., UV light) in the presence of a photosensitizer (e.g., riboflavin). Cancer immunotherapy involves the use of compositions and methods to elicit and enhance an individual's own immune system against cancerous cells, or infections that are factors predisposing to cancer.SELECTED DRAWING: None

The invention relates to a preparation method of cell-loaded silk fibroin hydrogel fibers and fibers, the preparation method of the cell-loaded silk fibroin hydrogel fibers comprises the following steps: mixing a silk fibroin solution with a riboflavin solution to obtain a mixed solution, adding a hydrogen peroxide solution into the mixed solution to obtain a precursor solution, and carrying out ultrasonic treatment on the precursor solution to obtain the cell-loaded silk fibroin hydrogel fibers. Adding stem cells into the precursor solution, and then carrying out photocuring reaction under the irradiation of an ultraviolet lamp to prepare the hydrogel fiber. The fiber is obtained through the preparation method. According to the preparation method of the cell-loaded silk fibroin hydrogel fiber and the fiber, the gel forming time is greatly shortened, efficient and uniform loading of cells is achieved, the survival rate of the cells is increased, no organic reagent residues exist in the preparation process, operation is easy, the technological process is short, and the method is suitable for industrial production. The prepared fiber is free of biotoxicity, excellent in mechanical property, good in light transmission and good in biocompatibility, and has certain potential and application prospects in the biomedical field.

According to the silk fibroin hydrogel microneedle manufacturing method and the hydrogel microneedle, a silk fibroin solution and a riboflavin aqueous solution are evenly mixed to obtain a mixed solution, then a hydrogen peroxide solution is added into the mixed solution to be mixed to obtain a precursor solution, then the precursor solution is poured into a light-transmitting microneedle mold, and the silk fibroin hydrogel microneedle is obtained. Placing a microneedle mold containing the precursor solution into a vacuum pump, performing vacuum pumping treatment, taking out the microneedle mold, placing the microneedle mold under ultraviolet irradiation for illumination to form a gel, drying the gel at room temperature, taking out the gel from the microneedle mold, forming a plurality of convex cones on the outer surface of the gel, and performing vacuum pumping treatment on the cones to obtain the composite microneedle. The silk fibroin hydrogel microneedle is obtained. According to the design, the operation requirement is low, the slow release effect is good, the biocompatibility is high, and efficient carrying of treatment medicine is facilitated.

The invention belongs to the technical field of microbial hydrogen production optimization, and particularly relates to a clostridium pasteurianum biological / non-biological hydrogen production system based on a hydrogen production mechanism. According to the system, an endogenous hydrogenase gene of clostridium pasteurianum is over-expressed in the clostridium pasteurianum, or a hydrogen production element is added in the culture process of the clostridium pasteurianum, and the hydrogen production element comprises one or more of Fe < 2 + >, Ni < 2 + >, riboflavin, humic acid or magnetic nanoparticles. According to the invention, the effects of different types of hydrogenase on hydrogen production of clostridium pasteurianum are explored, and the hydrogen production mechanism of clostridium pasteurianum is defined; by adding a new hydrogen production element externally, the hydrogen yield of the clostridium pasteurianum is further improved, the development of a biological hydrogen production technology can be promoted, and a feasible biological solution is provided for production of clean energy.

The disclosure provides a cancer vaccine composition comprising inactivated cancer cells and an adjuvant, wherein the inactivated cancer cells are incapable of replication. Also provided is a method for producing a cancer vaccine composition, the method comprising contacting cancer cells with light (e.g., UV light) in the presence of a photosensitizer (e.g., riboflavin).

The invention belongs to the field of environmental biotechnology and nano material application, and relates to a method for accelerating microorganisms to reduce selenite based on immobilized riboflavin. Aiming at the problems of poor stability, difficulty in recovery, non-repeated use and the like of dissolved riboflavin serving as an electron shuttle, riboflavin is grafted on the surface of a multi-walled carbon nanotube through a covalent binding method, and immobilized riboflavin capable of being recycled is constructed. The material can accelerate extracellular electron transfer of shewanella MR-1, and toxic sodium selenite is efficiently reduced into low-toxicity or non-toxic elemental selenium. Results show that the immobilized riboflavin not only maintains high reduction activity of riboflavin, but also has excellent cyclic utilization performance, still maintains high reduction rate after being repeated for multiple times, and effectively reduces operation cost and resource loss. According to the invention, an efficient, economical and sustainable application scene is provided for a microorganism-electron shuttle combined remediation technology, and a new thought is also provided for application of a functional nano material in an environmental biotechnology.

The invention provides a formula of a sports nutritious food and a preparation method thereof, and the formula comprises the following components: edible glucose, sorbitol, bitter gourd powder, Chinese yam powder, poria cocos powder, lucid ganoderma (ganoderma sinensis), ground cinnamon, oyster powder, licorice powder, thiamine hydrochloride and riboflavin. The food formula contains multiple natural food materials such as lucid ganoderma (ganoderma sinensis), bitter gourds, Chinese yams, poria cocos, cinnamon, oysters and liquorice, and nutritional ingredients such as edible glucose, sorbitol, thiamine hydrochloride and riboflavin, so that rich nutritional support can be provided for sports people, the body requirements of the sports people can be met, and the health of the sports people can be improved. According to the present invention, the edible glucose and the sorbitol can provide the rapid energy source for the body, the physical ability and the endurance of the sporter can be easily improved so as to improve the athletic performance, and the product is convenient to carry and eat after the packaging, and is suitable for the sporter to supplement the nutrition before and after the exercise or in the daily life at any time.

The invention discloses a preparation method of a NM88B-POR / Rf nanozyme and its application. The method first in-situ dopes porphyrin in NM88B(Fe) (NM88B-POR), and then loads riboflavin (Rf) on NM88B-POR by electrodeposition, while realizing the sarcosine oxidase (SOx) nanozyme Rf and peroxidase nanozyme NM88B(Fe) enzyme cascade reaction to enhance the sarcosine (Sar) detection signal, and also enhances the capture of oxygen molecules in aqueous solution through the heme-like effect of the Fe-POR structure in NM88B-POR to construct a high oxygen microenvironment, thereby realizing highly sensitive electrochemical detection of Sar. The present invention establishes a new Sar enzyme cascade electrochemical detection strategy, and the heme-like Fe-POR structure in the constructed electrochemical nanozyme can also build a high-oxygen microenvironment by enhancing the capture of dissolved oxygen, solving the problem that the current Rf nanozyme can only biomimetic the SOx active center structure and ignores the fact that the capture of dissolved oxygen during the reaction process can significantly improve the reaction rate. The nanozyme ultimately achieves ultra-sensitive electrochemical detection of Sar through the synergistic effect of enzyme cascade and high-oxygen microenvironment.

A formulation for an eye treatment includes a photosensitizer and a permeability enhancing composition. The permeability enhancing composition includes one or more permeability enhancers. The permeability enhancing composition has a hydrophilic and lipophilic balance increases a permeability of an area of the eye for the photosensitizer. The hydrophilic and lipophilic balance can be characterized by a Hydrophile-Lipophile Balance (HLB) number. For example, the area of the eye may include a corneal epithelium, the photosensitizer may include riboflavin, and the permeability enhancing composition may have a corresponding HLB number between approximately 12.6 and approximately 14.6.

The invention discloses a prefabricated photocatalyst film for retaining freshness of leaf vegetables as well as a preparation method and application of the prefabricated photocatalyst film. The photocatalyst film comprises the following components in parts by weight: 100 parts of chitosan; 0.5 to 2 parts of metal-nitrogen doped titanium dioxide; 0.5 to 1 part of riboflavin; 30 to 40 parts of glycerol; wherein the metal-nitrogen doped titanium dioxide is iron-nitrogen doped titanium dioxide and / or copper-nitrogen doped titanium dioxide. The photocatalyst film is used for sealing the prefabricated leaf vegetables, composite LED illumination in the cold chain distribution process is combined, the antibacterial and fresh-keeping effects are achieved, microbial infection of the prefabricated vegetables in the cold chain distribution process can be reduced, water loss and chlorophyll degradation can be reduced, and the shelf life of the prefabricated vegetables can be prolonged.

The invention discloses a selenium-rich camellia oil capsule capable of effectively improving a health-care effect and a preparation method thereof. The selenium-rich camellia oil capsule contains unsaturated fatty acid, saturated fatty acid, tea polyphenol, camellia saponin, squalene, 14-methylpentadecanoic acid, linoleic acid, stearic acid, linolenic acid, protein, cellulose, vitamin E, thiamine, riboflavin, nicotinic acid, magnesium, calcium, iron, zinc, copper, manganese, potassium, phosphorus and selenium. According to the technical scheme, the quality and the health-care effect of the camellia oil can be effectively improved, meanwhile, the loss of active ingredients is reduced, the oil yield is increased, and the nutritional ingredients of the camellia oil are kept. Besides, the method also has the advantage of environmental protection, as supercritical CO2 can be recycled, the influence on the environment is reduced, the selenium-enriched camellia oil capsule can stay in the intestinal tract of a human body for a long time, the absorption rate of effective components is improved, and unsaponifiable matters, such as gamma-tocopherol, beta-sitosterol and squalene, of the camellia oil are retained.

The invention discloses a degradable plastic quality release and demolding residue traceability management method, and relates to the technical field of quality release traceability, and the method comprises the following steps: 1, doping a fluorescent whitening agent European in a polylactic acid matrix to form an inner channel, attaching a polyvinyl alcohol witness sheet to the edge of a cavity, and presetting riboflavin to form an outer channel, establishing a dual-channel ultraviolet detection position; 2, collecting two paths of intensity, calculating a ratio coefficient, and establishing mapping with surface residues to form red, yellow and green threshold values and a correction list; 3, a workpiece is judged through a black box, spray volume, wind shear and mold temperature instructions are automatically given, a witness piece is separated from warm water and confirmed through a background, and meanwhile a cavity-level demolding event is generated and bound with the digital link two-dimensional bar code; and step four, third-party compliance files are archived in a ticket-folder manner with quantiles as reference to a reconvergence threshold value, so that mold-crossing and line-crossing consistent release and verifiable traceability are realized, and food contact and compostable boundaries are considered. And the release efficiency and stability are improved.

A black fungus cultivation material capable of increasing vitamin B2 belongs to the technical field of high-riboflavin black fungus strain breeding and cultivation, and is prepared by mixing basswood chips, quick lime, gypsum powder, purified water and a fermentation product, the fermented product is prepared by crushing and mixing the following components in parts by weight in the mixed powder 1: 10-12 parts of chrysanthemum morifolium ramat, 5-8 parts of ginkgo leaves, 3-6 parts of liquorice, 20-30 parts of pine cones and 15-18 parts of walnut shells into the mixed powder 1, adding bran shells, corn flour and sucrose powder, mixing to form mixed powder 2, sterilizing, and fermenting by adopting arabitol hansenula polymorpha, lactococcus lactis and bacillus licheniformis. According to the prepared cultivation material, the riboflavin content in the black fungus is effectively increased and reaches 2 mg / 100 g dry fungus or above, the cultivation period of the black fungus is shortened, meanwhile, the yield of the dry fungus reaches 120 g / bag or above, the cultivation method can be used for seedling cultivation of high-riboflavin black fungus strains, and high-quality high-riboflavin black fungus strains are obtained through cultivation of the cultivation material.

The invention relates to a livestock breeding technology, and discloses a preparation method of a premix for enhancing laying hen production treatment, in the construction of a basic nutrition framework, the construction of a vitamin system is a core project, vitamin A is supplemented by adopting a palmitate form, the stability of the vitamin A in feed processing is ensured, the level of 8000-12000IU should be maintained in each kilogram of feed, and the content of the vitamin A in the feed processing is reduced. According to the fallopian tube mucous membrane composition, the addition amount of the vitamin D3 in the metabolic activity form of 25-hydroxyD3 needs to reach 2000-3000 IU / kg, the vitamin D3 and parathyroid hormone have a synergistic effect, the calcium and phosphorus deposition efficiency can be remarkably improved, particularly, the synergistic effect of B vitamins is worthy of attention, and thiamine (B1) and riboflavin (B2) are compatible according to the proportion of 1: 2. According to the preparation method of the premix for enhancing laying hen production treatment, the growth speed and the laying efficiency of chicken flocks are effectively improved, the eggshell quality is ensured, nutritional diseases such as chondropathy and egg reduction diseases are reduced, and the economic benefits of laying hen breeding are greatly improved.

An instant fish maw processing technology for delaying degradation of collagen belongs to the technical field of instant fish maw processing, and comprises the following steps: S1, treating fish maw with 0.1% of tea polyphenol, 0.05% of a rosemary extract and 0.2% of chitosan oligosaccharide for 30 min, carrying out enzyme inhibition treatment, and then cleaning and draining for later use; s2, dipping the fish gelatin treated in the step S1 in a solution containing 0.1% of riboflavin and 0.2% of hyaluronic acid, irradiating the fish gelatin with white light for 10s, and then cleaning and draining the fish gelatin for later use; and S3, putting 5-7 parts by weight of fish maw and 65-80 parts by weight of pure milk into a container, sealing, and sterilizing to obtain the instant fish maw. Through enzyme inhibition treatment, the hydrolysis capability of metalloproteinase on collagen is reduced or eliminated, and degradation of the collagen is reduced, so that the nutritional value of the fish maw is maintained; collagen molecules in the fish maw and riboflavin are subjected to a cross-linking reaction to form a stable three-dimensional network structure, so that the acting force between the collagen molecules is enhanced, the structural stability of the collagen is improved, and the degradation of the collagen is delayed.

This invention relates to the field of functional food technology, and more particularly to the application of safflower honey, or safflower honey extract, in enhancing the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). The invention has found that safflower honey, or safflower honey extract, can effectively enhance ADH and ALDH activity, thereby promoting alcohol metabolism and achieving a hangover-relieving effect. Further research on the extract revealed that a mixture of syringaldehyde, riboflavin, lutein, luciferin, and three p-coumaroyl spermidine plays a key role in enhancing ADH and ALDH activity, thus providing an application of this mixture in hangover relief. The application of safflower honey and its extract provided by this invention has high application value for developing foods and health products with hangover-relieving effects.

The invention discloses an escherichia coli strain with high yield of riboflavin as well as a construction method and application of the escherichia coli strain. A series of Escherichia coli strains with high yield of riboflavin are constructed, the constructed Escherichia coli with high yield of riboflavin is safe and harmless, glucose is used as a substrate for fermentation under the aerobic condition of a shake flask, the yield of riboflavin is 1117.8 mg / L, 1330.1 mg / L, 1430.8 mg / L, 1508.7 mg / L and 1640.8 mg / L in sequence, and the yields are 112.5 mg / g of glucose, 134.1 mg / g of glucose, 146.3 mg / g of glucose, 158.8 mg / g of glucose and 168.1 mg / g of glucose in sequence; when the strain SN04M is subjected to fed-batch fermentation in a 1L fermentation tank, the yield of riboflavin reaches 8.52 g / L, and the total yield is 189.9 mg / g glucose. According to the invention, the fermentation yield of riboflavin and the substrate utilization rate of riboflavin to glucose can be obviously improved.

The present invention relates to a fluorescent inspection liquid which is highly safe to a human body and emits blue fluorescence having high visibility in the leakage inspection. The fluorescent inspection liquid contains water as a solvent and two blue fluorescent dyes selected from a flavin derivative, quinine, pyrene and anthocyanin. The flavin derivative is a derivative having a flavin skeleton and obtained from a starting material, riboflavin.

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.

The invention belongs to the technical field of solid beverages, and particularly discloses a functional solid beverage and a preparation method thereof.The solid beverage is composed of superfine mixed powder formed by conducting low-temperature baking, wall breaking treatment and screening on components in percentage by mass: 15% of sea-buckthorn, 15% of white mulberry, 12% of dandelion, 12% of radix polygonati officinalis, 12% of rhizoma polygonati, 30% of black fruit small molecule peptide, 1% of riboflavin and 3% of monosaccharide. The low-temperature baking is a treatment process in which each component is independently performed under the condition of 40-60 DEG C and the duration time is longer than 24 hours, and a synergistic technical scheme of differential pretreatment and accurate low-temperature baking effectively solves the technical problem that active components are degraded due to high-temperature and non-protection treatment in traditional solid beverage processing; in the raw material pretreatment stage, plant-derived raw materials such as hippophae rhamnoides and white mulberries are pre-dried at the temperature of 50-60 DEG C for humidity control, and polygonatum kingianum is steamed for 2-3 hours to convert irritant components.

The invention belongs to the technical field of vitamin B2 production, and particularly relates to a preparation method of a safe high-yield strain for vitamin B2 production, which comprises the following steps: S1, strain source and screening: analyzing the current situation and development trend of vitamin B2, and screening the strain; s2, genetic engineering modification: evaluating the production performance and genetic stability of the strain, and carrying out mutagenesis treatment on the screened strain by using a chemical mutagenic agent to obtain a genetically mutated strain; s3, screening and identifying high-yield mutant strains: integrating the fragments containing the spore cereus dry riboflavin operon into a vitamin B2 genome to generate the mutant strains, and performing fine adjustment on the riboflavin operon in the mutant strains by utilizing a multiple genome editing system. And through technical popularization, the manufacturing cost of the product can be reduced, and the social benefit is obvious.

The present invention is related to an improved process for production of vitamin B2 using a genetically engineered host cell comprising a heterologous enzyme with pyridoxal phosphatase activity. Using said modified host cell, the yield of riboflavin production could be increased by at least about 5%.