30 results about "Environmental biotechnology" patented technology

The invention belongs to the technical field of environmental biology, and particularly relates to a prothioconazole methyltransferase gene proS38 and application thereof. The prothioconazole methyltransferase proS38 gene in the Sphingomonas sp.AJ-1 strain and the prothioconazole methyltransferase which is a protein expression substance thereof are found through analysis, screening, identification and verification, the prothioconazole methyltransferase proS38 gene and the prothioconazole methyltransferase which is a protein expression substance thereof are proved to have efficient and specific degradation catalysis capability on the existing pesticide prothioconazole which is used in a large amount, and a foundation is laid for biodegradation of prothioconazole.

The invention discloses a staged regulation and control sulfur cycle enhanced denitrification microbial community construction method, and belongs to the technical field of environmental biologication.The method comprises the three stages that in the first stage, low-carbon-nitrogen-ratio domestication culture is conducted, the carbon-nitrogen ratio is 1.5-2.5: 1, and domestication is conducted for 50-70 days; in the second stage, electron donor-acceptor ratio regulation culture is carried out, the / molar ratio is (4-6): 1, and culture is carried out for 15-25 days; in the third stage, pulse type sulfate is fed for enhanced culture, the single-time feeding concentration is 200-500 mg / L, culture is conducted for 8-15 days, real-time monitoring is conducted through the fluorescence in-situ hybridization technology, construction is completed when the abundance of sulfur autotrophic denitrifying bacteria reaches 28% or above, and when the microflora constructed through the method is used for treating low-carbon-nitrogen-ratio industrial wastewater, the sulfur conversion rate is 1.2-2.0 kg / (m.d), the system stability is good, and the application range is wide. And the impact load resistance is high.

The invention belongs to the field of environmental biotechnology and nano material application, and relates to a method for accelerating microorganisms to reduce selenite based on immobilized riboflavin. Aiming at the problems of poor stability, difficulty in recovery, non-repeated use and the like of dissolved riboflavin serving as an electron shuttle, riboflavin is grafted on the surface of a multi-walled carbon nanotube through a covalent binding method, and immobilized riboflavin capable of being recycled is constructed. The material can accelerate extracellular electron transfer of shewanella MR-1, and toxic sodium selenite is efficiently reduced into low-toxicity or non-toxic elemental selenium. Results show that the immobilized riboflavin not only maintains high reduction activity of riboflavin, but also has excellent cyclic utilization performance, still maintains high reduction rate after being repeated for multiple times, and effectively reduces operation cost and resource loss. According to the invention, an efficient, economical and sustainable application scene is provided for a microorganism-electron shuttle combined remediation technology, and a new thought is also provided for application of a functional nano material in an environmental biotechnology.

The invention relates to the technical field of molecular biology and environmental biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of functional genes related to microbial degradation of chlorinated hydrocarbon and application of the PCR chip and the kit. The PCR chip comprises a primer pair and a primer pair, the primer pair is used for specifically amplifying dehalogenation functional genes bvcA, cfrA, mbrA, pceA, pteA, tceA and vcrA respectively, the primer pair is used for specifically amplifying reference genes, and nucleotide sequences of the primer pair for specifically amplifying the dehalogenation functional genes are shown as SEQ ID NO. 1 to SEQ ID NO. 14. The PCR chip provided by the invention can specifically amplify seven functional genes related to the degradation of the chlorinated hydrocarbon microorganisms, is good in specificity, high in accuracy and good in repeatability, and can accurately detect the expression condition of the seven dehalogenation functional genes related to the degradation of the chlorinated hydrocarbon microorganisms through a qPCR technology, so that the existence condition of chlorinated hydrocarbon degrading bacteria can be reflected; the method has reliability in the aspects of chlorohydrocarbon polluted site biodegradability evaluation, degradation process research, repair effect verification and the like.

The invention relates to an intelligent pollutant treatment method and system and a medium in the crossing field of environmental biotechnology and artificial intelligence, and the intelligent pollutant treatment method comprises the following steps: obtaining historical data of a composting system, and carrying out preprocessing; constructing an LSTM neural network prediction model, training the LSTM neural network prediction model by using the preprocessed historical data, and establishing prediction model parameters to obtain a trained LSTM neural network prediction model; acquiring real-time monitoring data of to-be-predicted compost, and preprocessing the real-time monitoring data; taking the preprocessed real-time monitoring data as the input of a trained LSTM neural network prediction model to obtain prediction data; and based on the prediction data, according to a preset optimization target, adjusting operation parameters of the composting system. According to the intelligent pollutant treatment method, the pollutant degradation efficiency of the composting system can be improved, the pollutant treatment effect is improved, and the composting quality is improved.

The invention relates to the technical field of bioengineering and environmental biology, and particularly discloses high-temperature-resistant polylactic acid depolymerases and application thereof in degradation of polylactic acid plastics. The nucleotide sequence of the coding gene of the high-temperature-resistant polylactic acid depolymerases B01 is as shown in SEQ ID NO: 1, and the amino acid sequence of the coding gene is as shown in SEQ ID NO: 2. The enzyme is derived from metagenome mining, the sequence similarity of the enzyme and the known PLA depolymerases PAM is only 34.45%, and the enzyme has remarkable novelty. According to the present invention, the secretory expression and the purification of the B01 are successfully achieved in the bacillus subtilis SCK6; an enzymatic property research shows that the optimal reaction temperature of the B01 is 65 DEG C, the half-life period at 60 DEG C is as long as 150 minutes, and the B01 shows excellent thermal stability; and the optimum pH value of the strain is 9.0. The invention provides a novel enzyme preparation with important application potential for high-temperature biological recovery treatment of PLA plastic waste.

The invention belongs to the technical field of environmental biology, and particularly relates to a key gene for degrading skatole by enterococcus haiseri ID05 and application of the key gene for degrading skatole by enterococcus haiseri ID05, and the test process of the key gene for degrading skatole by enterococcus haiseri ID05 is as follows: selecting an enterococcus haiseri IDO5 strain, an LB culture medium, an inorganic salt culture medium and reagent consumables and instruments required in the test process; the degradation rate of the enterococcus haiseri IDO5 on 100 mg / L of skatole in an inorganic salt culture medium reaches 67.01 + / -0.01%, and it is shown that the enterococcus haiseri IDO5 has the good degradation capacity on the skatole. Besides, the degradation rate of the IDO5 on skatole in sterilized pig manure wastewater reaches up to 96.10%, the degradation rate of the IDO5 on skatole in unsterilized pig manure wastewater is also 78.17%, and the enterococcus hirsuti IDO5 has good degradation capacity on skatole in pig manure wastewater and has good application potential in pig manure wastewater, so that when the pig manure wastewater is discharged, the enterococcus hirsuti IDO5 can be used for degrading the skatole in the pig manure wastewater. After being treated by enterococcus hirsuti IDO5, the wastewater can be discharged, so that the pollution to the environment is effectively reduced.

The present application belongs to the field of environmental biotechnology and resource recycling technology, and provides a high-ammonia-nitrogen-tolerant yeast strain and its application in biogas slurry resource treatment. The high-ammonia-nitrogen-tolerant yeast strain is named Torulaspora delbrueckii L12345, which was preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 13, 2026, and the address is No. 1, Yitian West Road, Chaoyang District, Beijing, China Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No. 39123. The present application realizes efficient treatment of biogas slurry and simultaneous production of single-cell protein, reduces the treatment cost, improves the economic benefit, and provides a new technology for clean and high-value utilization of biogas slurry.

The present application belongs to the field of environmental biotechnology, and particularly relates to prothioconazole methyltransferase gene pmt80 from mycobacterium and application thereof. The present application finds a methyltransferase gene pmt80 for effective degradation of prothioconazole and a corresponding enzyme pmt80 expressed by recombination of the methyltransferase gene pmt80, and the catalytic efficiency of the enzyme for (R)-PTC reaches 3.4 x 10 ‑3 min ‑1 μM ‑1 The optimal temperature of the enzyme for catalyzing (R)-PTC is 37 DEG C, and the reaction condition is mild; meanwhile, the pmt80 of the present application can catalyze degradation of PTC and 4-ethyl-5-phenyl-4H-[1,2,4]triazole-3-thiol which is an analog of PTC. In summary, the prothioconazole methyltransferase found by the present application has good industrial application potential in environmental pollution control.

The invention provides a multi-enzyme combination for accurately characterizing a compost fermentation process and application, and belongs to the technical field of environmental biotechnology and solid waste resource utilization, the multi-enzyme combination comprises alpha-glucosidase, alpha-galactosidase, endo-1, 3-glucanase, endo-cellulase and endo-chitosanase. The multi-enzyme composition is determined by screening composting systems of different raw materials such as wheat straw, corn straw, chicken manure, alfalfa and sludge under different C / N ratios. The multi-enzyme combination can accurately characterize the four stages of temperature rise, high temperature, temperature reduction and decomposition of the compost, the enzyme activity can be determined through a plurality of detection methods such as a fluorescent color development method and a visible light color development method, so that the real-time monitoring and state evaluation of the compost process are realized, the technical support for compost process regulation and control and quality release is provided, and the application prospect is wide.

The invention provides a complex microbial inoculant and application thereof in degradation of starch and biofuel cells, and belongs to the cross technical field of environmental biotechnology and bioelectrochemistry. According to the invention, a complex microbial inoculant composed of three microorganisms is constructed through a synthetic biological strategy; the complex microbial inoculant comprises genetically engineered Escherichia coli capable of rapidly converting starch into monosaccharide easy to use, shewanella for bearing upstream metabolites and geobacter for generating electricity, wherein the genetically engineered Escherichia coli is used for rapidly converting starch into monosaccharide easy to use; according to the invention, through precise function division of three strains, a complex degradation and power generation process is decomposed into a plurality of sub-steps catalyzed by special strains, so that an efficient'metabolic assembly line 'is formed, the inhibition of intermediate products is reduced, and the overall energy conversion efficiency is improved; the complex microbial inoculant can be used for degrading starch and preparing microbial fuel cells, realizes wastewater purification and recovery of bioelectric energy at the same time, and has remarkable environmental and economic benefits.

The invention relates to the technical field of environmental biology, and discloses a short-cut nitrification-denitrification complex microbial inoculant and a preparation method thereof. The method includes; the method comprises the following steps: respectively culturing ammonia oxidizing bacteria, denitrifying bacteria and heterotrophic nitrification-denitrifying bacteria, mixing, and co-domesticating under a low dissolved oxygen condition to obtain a co-domesticated flora; and then adding into sodium alginate-polyvinyl alcohol, immobilizing by a calcium chloride-boric acid-glutaraldehyde system, and freeze-drying to obtain the complex microbial inoculant. The prepared complex microbial inoculant can stably realize synchronous short-cut nitrification and denitrification under the condition of low dissolved oxygen, is high in nitrite accumulation rate, remarkably improves the removal rate of ammonia nitrogen and total nitrogen, has the advantages of stable flora structure, high denitrification efficiency and low operation energy consumption, and is suitable for industrial production. The method can be widely applied to biological nitrogen treatment of urban sewage and high-ammonia-nitrogen industrial wastewater.

This invention belongs to the field of environmental biotechnology, specifically, it relates to a prothioconazole methyltransferase gene. proS38 And its applications. This invention, through analysis, screening, identification, and verification, discovered... Sphingomonas Prothiozoxystrobin methyltransferase in sp. AJ-1 strain proS38 The gene and its protein expression product, prothioconazole methyltransferase, have been shown to have a highly efficient and specific degradation catalytic ability for the widely used pesticide prothioconazole, laying the foundation for the biodegradation of prothioconazole.

The application discloses a method for intensively degrading methane by using a peristaltic pump to drive immobilized bacteria, and belongs to the field of environmental biotechnology. The method comprises the following steps: immobilizing methane-oxidizing bacteria on the surface of a porous carrier such as a metal organic framework material, activated carbon or alumina; constructing a closed circulation device composed of a gas storage bag, a peristaltic pump and a culture tube; and intensifying methane mass transfer by using pulsating flow generated by the peristaltic pump. The method overcomes the defects of large gas-liquid mass transfer resistance, easy loss of bacteria and complicated operation in traditional methane-oxidizing bacteria culture. Under the optimal conditions, the methane oxidation rate can reach 12.9 micromoles / h, which is significantly higher than that in static culture.

The invention belongs to the technical field of environmental biology, and particularly relates to a prothioconazole methyltransferase gene proS32 and application thereof. According to the invention, a key enzyme prothioconazole methyltransferase proS32 gene of the strain Sphingomonas sp. AJ-1 for degrading prothioconazole is found through biological information analysis and gene cloning, the prothioconazole methyltransferase ProS32 is obtained through expression of an expression vector, and the prothioconazole methyltransferase ProS32 is proved to have efficient and specific degradation catalysis capability on prothioconazole by utilizing HPLC (High Performance Liquid Chromatography) and enzymatic detection; and a foundation is laid for developing a corresponding biodegradation reagent or developing efficient degradation bacteria.

The invention discloses a waste water toxicity grading detection system based on the quorum sensing principle and an operation and maintenance guidance method, and belongs to the technical field of environmental biotechnology and waste water treatment monitoring, and the waste water toxicity grading detection system based on the quorum sensing principle comprises a biological sensing module, the biosensor is used for generating specific signal molecules through quorum sensing microbial strains so as to respond to the toxicity of wastewater; the signal detection module is used for detecting the concentration of the specific signal molecules or a corresponding electrochemical signal; and the control and data analysis module is used for controlling reaction conditions, collecting and processing signal data and calculating the toxicity inhibition rate. According to water quality characteristics of different nodes in a wastewater treatment process, different quorum sensing strains are selected, and signal molecules are detected in situ through a high-sensitivity electrochemical sensor, so that rapid, accurate and graded evaluation of toxicity is realized, and further advanced early warning and accurate decision support are provided for system operation and maintenance.

The invention belongs to the technical field of environmental biology, and particularly discloses a microorganism dechlorination method for enhancing combined pollution of microplastics and polychlorinated biphenyl. The method comprises the following step: adding natural humin and peptone into a coexisting culture system containing polychlorinated biphenyl, anaerobic dechlorination microorganisms and microplastics. The invention discloses a microbial dechlorination method for enhancing combined pollution of microplastics and polychlorinated biphenyl, which is characterized in that natural humin and peptone are applied to a microplastics and polychlorinated biphenyl (PCBs) coexisting microbial dechlorination culture system for the first time, and the natural humin and peptone are combined to generate a remarkable synergistic effect; the method can effectively relieve the inhibition of microplastics on the dechlorination activity of microorganisms, and provides a brand new and efficient solution for biological enhancement repair of typical combined pollution environments such as estuaries, sediments and the like.

The application provides a multi-enzyme combination and application for accurately characterizing compost fermentation process, and belongs to the technical field of environmental biotechnology and solid waste resource utilization, and the multi-enzyme combination comprises alpha-glucosidase, alpha-galactosidase, endo-1, 3 glucanase, endo-cellulase and endo-chitosanase. The multi-enzyme combination is determined by screening the compost systems of different raw materials such as wheat straw, corn straw, chicken manure, alfalfa, sludge and the like under different C / N ratios. The multi-enzyme combination can accurately characterize four stages of composting, i.e. temperature rising, high temperature, temperature falling and maturity, and the enzyme activity can be determined by various detection methods such as fluorescence colorimetry and visible light colorimetry, so that the real-time monitoring and state evaluation of the composting process can be realized, technical support for the process regulation and quality release of composting is provided, and the application prospect is wide.

The invention belongs to the technical field of environmental biology, and particularly relates to a prothioconazole methyltransferase gene proS18 and application thereof. According to the invention, a key enzyme prothioconazole methyltransferase proS18 gene of the strain Sphingomonas sp. AJ-1 for degrading prothioconazole is found through biological information analysis and gene cloning, the prothioconazole methyltransferase ProS18 is obtained through expression of an expression vector, and the prothioconazole methyltransferase ProS18 is proved to have efficient and specific degradation catalysis capability on prothioconazole by utilizing HPLC (High Performance Liquid Chromatography) and enzymatic detection; and a foundation is laid for developing a corresponding biodegradation reagent or developing efficient degradation bacteria.

This invention belongs to the field of environmental biotechnology, and in particular, it relates to the prothioconazole methyltransferase gene. proS18 Its applications. This invention discovered strains through bioinformatics analysis and gene cloning. Sphingomonas The key enzyme in sp. AJ-1 that degrades prothioconazole is prothioconazole methyltransferase. proS18 The gene was obtained and prothioconazole methyltransferase ProS18 was expressed through an expression vector. HPLC and enzymatic detection confirmed its efficient and specific degradation catalytic ability for prothioconazole, laying the foundation for the development of corresponding biodegradation reagents or efficient degrading bacteria.

The invention belongs to the technical field of environmental biotechnology and resource recycling, and provides high ammonia nitrogen resistant saccharomycetes and application thereof in biogas slurry resourceful treatment, the name of the high ammonia nitrogen resistant saccharomycetes is Torricaspora delbrueckii L12345, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 13, 2026, and the preservation number of the strain is CGMCC NO. The address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.39123. The method realizes efficient treatment of the biogas slurry and synchronous production of the single-cell protein, reduces the treatment cost, improves the economic benefit, and provides a new technology for clean and high-valued utilization of the biogas slurry.

This invention belongs to the field of environmental biotechnology, and in particular, it relates to the prothioconazole methyltransferase gene. proS32 Its applications. This invention discovered strains through bioinformatics analysis and gene cloning. Sphingomonas The key enzyme in sp. AJ-1 that degrades prothioconazole is prothioconazole methyltransferase. proS32 The gene was obtained and prothioconazole methyltransferase ProS32 was expressed through an expression vector. HPLC and enzymatic detection confirmed its efficient and specific degradation catalytic ability for prothioconazole, laying the foundation for the development of corresponding biodegradation reagents or efficient degrading bacteria.

The invention relates to the technical field of environmental biotechnology and wastewater treatment, in particular to a culture method of salt-tolerant aerobic granular sludge and a degradation method of industrial wastewater, the culture method comprises the following steps: S1, inoculating initial aerobic granular sludge into a bioreactor; and S2, preliminarily adding acrylamide and an auxiliary carbon source into the bioreactor obtained in the step S1, and then increasing the acrylamide and reducing the addition amount of the auxiliary carbon source for domestication to obtain the salt-tolerant aerobic granular sludge. The salt-tolerant aerobic granular sludge cultured by the invention can grow and metabolize in an environment in which the sodium chloride concentration is not less than 10g / L by taking acrylamide as a unique carbon source, so that the salt-tolerant aerobic granular sludge has excellent salt tolerance and efficient acrylamide degradation capacity, and can degrade more than 95% of 200mg / L acrylamide within 6 hours; meanwhile, the salt-tolerant aerobic granular sludge is regular in particle shape, compact in structure and good in settling performance (the SVI (sludge index) value is only 24.0 mL / g).

The application relates to the fields of molecular biology and environmental biotechnology, and discloses a PCR chip, a kit and application thereof. The PCR chip comprises a chip plate, primer pairs for specifically amplifying bvcA genes, cfrA genes, mbrA genes, pceA genes, pteA genes, tceA genes, vcrA genes and internal reference genes are loaded on the chip plate, and a gradient dilution template of an internal reference gene for drawing a standard curve is arranged on the chip plate; the gradient dilution template of the internal reference gene is obtained by gradient dilution of internal reference gene template DNA and addition of internal reference gene primers. The PCR chip can detect absolute quantification of chlorinated hydrocarbon microbial degradation related reduction dehalogen functional genes through qPCR technology, so that the PCR chip can be used for accurately detecting the copy number of the chlorinated hydrocarbon microbial degradation related reduction dehalogen functional genes, thereby the feasibility of chlorinated hydrocarbon pollution bioremediation can be explored, the chlorinated hydrocarbon pollution bioremediation process can be evaluated, and the chlorinated hydrocarbon pollution bioremediation method can be further assisted and guided.

The invention relates to the technical field of environmental biology, in particular to a biogas slurry tolerance duckweed screening method based on multi-physiological index collaborative evaluation, which comprises the following steps: step 1, duckweed variety preparation: selecting a plurality of duckweed varieties; 2, biogas slurry pretreatment: precipitating and filtering the original biogas slurry to prepare test biogas slurry; step 3, tolerance test: respectively inoculating each duckweed variety into the test biogas slurry; 4, multi-physiological-index collaborative measurement, wherein the physiological indexes of all the duckweed varieties are measured at the same time; and 5, collaborative evaluation and screening: based on an analytic hierarchy process, establishing a comprehensive evaluation model, and carrying out quantitative evaluation and graded screening on the tolerance of the duckweed variety by utilizing the comprehensive evaluation model. According to the method, a comprehensive evaluation system of four indexes including chlorophyll stability, soluble sugar accumulation, electrolyte relative exosmosis rate and fresh weight change is constructed, and a classification screening standard and comprehensive tolerance index calculation model is established, so that the biogas slurry tolerance of the duckweed variety is accurately evaluated.

The invention discloses alkali-resistant polylactic acid depolymerases and application thereof in degradation of polylactic acid plastics, and belongs to the technical field of bioengineering and environmental biology, an amino acid sequence of the alkali-resistant polylactic acid depolymerases E01 is shown as SEQ ID NO: 2, and a nucleotide sequence of a coding gene of the alkali-resistant polylactic acid depolymerases E01 is shown as SEQ ID NO: 1. The enzyme is derived from metagenome mining, the sequence similarity of the enzyme and the known PLA depolymerases PAM is lower than 25%, the optimal reaction temperature of E01 is 50 DEG C, the optimal pH is 10.0, and the enzyme shows excellent stability within the alkaline range of pH 8.0-11.0 and under the condition of 50 DEG C. Degradation experiments prove that E01 has remarkable degradation capacity on various commercially available PLA products (including films, straws and meal boxes), and obvious lactic acid monomer release and material surface erosion can be observed after the PLA products are treated for 48 hours under the conditions that the temperature is 50 DEG C and the pH value is 10.0. The invention provides a novel enzyme preparation with important application potential for biological recovery treatment of PLA plastic waste.

The invention belongs to the technical field of environmental biology, and particularly relates to a deep sea hydrogen sulfide oxidizing bacterium and application thereof in a desulfurization process. The invention provides a deep sea hydrogen sulfide oxidizing bacterium (Sulfibacterium sp. B-2, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.35087), and a preparation method of the deep sea hydrogen sulfide oxidizing bacterium (Sulfibacterium sp. B-2). Meanwhile, the invention provides a biological desulfurization method and constructs a set of biological desulfurization process device, so that the content of hydrogen sulfide in gas and liquid can be effectively reduced, the environmental pollution is reduced, and renewable energy sources are obtained through purification. The biological desulfurization method provided by the invention can be used for desulfurization in low-temperature, high-salt, strong-alkali and other environments, and opens up a brand-new path for the innovation of the biological desulfurization process technology in the extreme environment through directional regulation and control of sulfur metabolites.

The invention belongs to the field of environmental biotechnology and wastewater biological treatment, and discloses a Feammox flora for anaerobic reduction defluorination of perfluorooctanoic acid and application of the Feammox flora. The Fammox flora is obtained by taking activated sludge as an inoculum, taking ammonium salt as an electron donor under an anaerobic condition and taking a ferric iron compound as an electron acceptor through enrichment and domestication; and the Feammox flora is used for reducing, defluorinating and degrading the perfluorooctanoic acid under an anaerobic condition. The method comprises the following steps: inoculating the Fammox flora into perfluorooctanoic acid-containing water to be treated, and reacting under an anaerobic condition in the presence of ammonium salt and ferric iron compounds. According to the invention, the Fammox composite flora which is domesticated from common activated sludge and does not depend on Acidimicrobium sp. A6 is used for the first time, efficient anaerobic biodegradation of PFOA is realized, microbial resources for PFAS bioremediation are broadened, and the Fammox composite flora has the characteristics of high efficiency, tolerance, wide application prospect and the like.

The application discloses a kind of efficient hexavalent chromium reductase mutant and its application in chromium-containing wastewater, belongs to environmental biotechnology field, with wild-type hexavalent chromium reductase SOYE from Streptomyces sp. M7 as parent, by random mutation and site-directed mutation combination modification, obtain three kinds of high-activity mutant, wherein mutant C25G / I71G / D75M / V159I / S196P / D260A / P298G / A310P / G347A catalytic efficiency is increased by 92.1% compared with wild type. With the mutant as active ingredient, using chitosan-nano Fe3O4 composite carrier to prepare immobilized enzyme preparation, its half-life is prolonged, and it can be reused more than 8 times, a composite catalytic system containing the immobilized preparation and NADH electron donor is constructed, and the treatment rate of 185mg / L chromium-containing wastewater is 99.77% after 2h treatment, and the effluent Cr (VI) concentration after actual wastewater treatment is lower than the national standard.

The invention belongs to the technical field of environmental biology, and discloses an ecological prevention and control method for constructing an antibiotic resistance gene transmission barrier by using indigenous microorganism diversity. In order to overcome the defects in the prior art, the invention provides an innovative method based on soil microbial diversity regulation and control. The core of the method lies in that diverse soil microbial communities with stable functions are actively constructed, and ecological barrier functions such as internal competition rejection, resource competition and interference / degradation capability to movable genetic elements are enhanced, so that the horizontal transfer frequency and diffusion range of endogenous and exogenous antibiotic resistance genes (ARGs) of soil are effectively inhibited, and the growth of the soil is promoted. The long-term resistance and the restoring force of a soil ecological system to the ARGs are enhanced, and an eco-friendly and efficient solution is provided for sustainable control of the antibiotic resistance of the environment.