The invention relates to a green bioactive organic fertilizer, which effectively solves the problems of soil hardening, environmental pollution, and harm from residual chemical substances of grains, vegetables and fruits to human bodies. The solved technical scheme is that the green bioactive organic fertilizer is prepared by taking sludge, waste powder, poultry and animal feces, straw powder, residues, feather powder, amino acid original powder, phosphoesterases complex, a first group of complex bacteria, and a second group of complex bacteria as raw materials; uniformly mixing the sludge, the waste powder, the poultry and animal feces, the straw powder, the phosphoesterases complex and the first group of complex bacteria; stacking and covering the mixture with a sack for performing a biogenic reaction, and keeping a reaction product at the temperature of 50 to 55 DEG C for 2 days; adding the residues, the feather powder, the amino acid original powder into the reaction product, uniformly mixing the mixture for performing a secondary reaction, and keeping a reaction product at the temperature of 50 to 55 DEG C for 7 to 10 days; and adding the second complex bacteria into the latest reaction product, uniformly mixing, palletizing, checking and packaging the mixture. The green bioactive organic fertilizer has the advantages of scientific formulation, simple preparation method, good effect, no pollution and no harm to human bodies.