279 results about "Arbuscular mycorrhizal fungi" patented technology

The invention discloses a microbial fertilizer producing by using agricultural wastes and preparation thereof. Main materials are obtained by mixing and smashing 20%-40% oil sally bean dregs, 40%-60% crop straw and 15%-25% poultry excrement, moisture content achieves 60-65%, composite leaven is added according to 150g-200g/T to pile to be cone-shaped, yellow mud is used on the outside for sealing, a plastic film is used for covering in a sealing mode, after stacking, fermentation and digesting, and the composite leaven is composed of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride according to the mass ratio (W/W) of 1.5:1:1:2.5:4. Auxiliary materials made of 5%-10% mushroom dregs and 6%-10% rice husk ash are evenly mixed with fermented and prepared main materials, arbuscular mycorrhizal fungi agent are added according to 600-1000g/T for compounding, and the microbial fertilizer is prepared after passing a 3mm sieve. Good production and efficiency improvement is obtained through application, and the microbial fertilizer has good application value.

The invention relates to a combined restoring method of polycyclic aromatic hydrocarbon contaminated soil, comprising the following restoring steps of: planting lucerne as perennial leguminous plants in the polycyclic aromatic hydrocarbon contaminated soil; inoculating a mixed bacterial inoculum comprising arbuscular mycorrhizal fungi, bacillus and flavobacterium; and meanwhile, adding rhamnolipid as a biosurfactant. By rhizosphere effect generated by plant root exudates, the number and the metabolic activity of soil microorganisms are increased; and by inoculating the arbuscular mycorrhizal fungi, the plant growth is promoted, and the root exudates are increased, thereby increasing the root accumulation of organic pollutants and the number of root soil microorganisms. The inoculated high-efficiency polycyclic aromatic hydrocarbon degrading bacteria use plant root secretions as a carbon source and an energy source and can quickly grow and propagate. The polycyclic aromatic hydrocarbon is stripped from soil grains by the biosurfactant, and the bioavailability and the degradability of the organic pollutants are increased.

A method of plant cultivation which comprises inoculating plants in a plant growing system comprising a container and an insert therefore, the microbial inoculant containing at least one species from the following group of microorganisms comprising arbuscular mycorrhizae associated bacteria, plant growth promoting rhizobacteria, yeast microorganisms and substrate conditioning bacteria.

The invention provides an application of arbuscular mycorrhizal fungi (AMF) in enhancement of growth and capability of heavy metal absorption from soil of black nightshade and a method of enhancing cadmium absorption of black nightshade from soil by utilization of the arbuscular mycorrhizal fungi. A cadmium (Cd) hyperaccumulating plant black nightshade is adopted as an experiment plant, the AMF is adopted as an Inoculant, the Cd hyperaccumulating plant black nightshade and the AMF capable of promoting plant growth are combined, and the AMF can enhance growth and capability of cadmium absorption from soil of the black nightshade, thus increasing the remediation efficiency of the black nightshade for Cd-contaminated soil. Compared with traditional chemical and agricultural measures, the method has characteristics of good treatment effects, no secondary pollution, low operation cost, and the like, and has good theoretical and application popularization value.

The invention relates to a compound soil remediation agent containing microorganism bacteria and a preparation method thereof. The remediation agent comprises the following components in parts by weight: 0.01-3 parts of bacillus pasteurii spore powder, 5-50 parts of phosphogypsum powder, 10-60 parts of organic compost, 5-50 parts of turf powder, 1.5-5 parts of potassium fulvate powder, 0.2-0.5 part of powdery bacteria powder carriers and 0.15-1 part of glycerin. The remediation agent has round and smooth grains and high grain hardness, is harmless to seeds, can serve as a base fertilizer together with other fertilizers, can be sown together with seeds, integrates multiple remediation functions including organic complexation and passivation remediation, inorganic passivation remediation, microorganism passivation remediation and the like, has a comprehensive and strong soil remediation function, and can stimulate growth and reproduction of arbuscular mycorrhizal fungi at the roots of crops, accelerate heavy metal passivation, stimulate crop growth and enhance the immunities of crops.

The present invention relates to Glomus mosseae 93-6 as one kind of efficient drought-resisting and high phosphate-tolerant nutrients bush mycorrhizal fungus and its production process. The Glomus mosseae preparation is prepared with kaoliang as host plant, mixture of zeolite and sand as culture medium. The dried preparation contains 40-65 spores in each ml. Glomus mosseae may be grown in various kinds of plants to improve nutrients absorption, raise drought resistance and promote growth of plant. Test shows that the present invention has excellent seedling strengthening and yield increasing effect on straw berry, rice, water melon and other crops.

A method for inducing the drought resistance of cucumbers by arbuscular mycorrhizal fungi comprises the following steps: carrying out individual inoculation and propagation expanding initial arbuscular mycorrhizal fungi inoculants; cultivating cucumber seedlings through mycorrhization of arbuscular mycorrhizal fungi inoculants, wherein inoculation media adopts a mixture of a Hoagland nutrient solution and perlite; inducing drought resistance: after the seedlings are grown for 3 months, transferring the seedlings in black seedling raising bags with the specification of 25 cm*25 cm, filling 3.0 kg of sterilization soil in each black seedling raising bag, applying 80 mg/kg of total phosphorus and 100 mg/kg of total nitrogen in each basin, and keeping the relative water content of the soil to be about 40% of the field capacity under moderate drought; sufficiently watering before processing to enable the seedlings to be in the natural drought, carrying out the water control processing when the natural drought reaches the preset relative water content in processing, using a weighing water supplementing method to keep the all repeated soil water content in each-time processing to be within the test required range during the water control period, setting the regulating time of the water content to be 8: 00 Am each day, and supplementing water lost on a previous day.

The invention discloses a method for improving the phytoremediation efficiency of low-concentration arsenic-contaminated soil, which comprises the steps of: planting corns in an arsenic-contaminated farmland, inoculating arbuscular mycorrhizal fungi and earthworms, pulling up the corns with roots after the growth season of the corn is over, harvesting the corns wholly, and performing centralized treatment. The method fully exploits the potentials of arsenic remediation of plants, microorganisms and animals, and inoculates the AM fungi and the earthworms to promote the plant absorption and dissipation of arsenic by promoting the growth of the plants, improving the activity of phosphatase of the soil and activating the arsenic in the soil to further achieve the aim of improving the phytoremediation efficiency of the low-concentration arsenic-contaminated soil. The method combines the microorganisms, the animals and the plants together for the in-situ remediation of the arsenic-contaminated farmland, has simple and convenient operation and friendly operating environment, cannot cause secondary pollution, reduces the treatment cost, and has good environmental effect.

The invention provides a method for locally culturing and producing arbuscular mycorrhiza fungus biological agent fertilizer in farmlands. The method is characterized by comprising the following steps of: 1, preparing a farmland culturing pond and a culture medium; 2, transplanting and culturing arbuscular mycorrhiza fungi; 3, absorbing and converting biological fertilizer; and 4, collecting biological fertilizer. Compared with the prior art, the technique adopted in the method has the advantages that: 1, the raw materials are readily available, the culture of the raw materials is easy, and the cost is low; 2, the culture technique and the production technique are conveniently popularized in rural areas; and 3, the production technique is green and environment friendly, which facilitates the development of modern agriculture. In addition, by using the fertilizer of the invention, the used amount of the quick-acting fertilizer is reduced and the used amount of partial fertilizer is reduced, so that the pollution to soil, water resources and air is lowered, and the fertilizer is particularly suitable to be used for planting seedlings of farm crops and medicinal plants. Tests show that the yield of the farm crops cultured by using the fertilizer of the method is improved by over 50 percent compared with that of the farm crops cultured by using the common fertilizer.

The present invention belongs to the technical field of crop organic biological fertilizer, in order to solve the increasingly seriously continuous cropping obstacle of vegetable planting, lighten the occurrence of disease and improve the output and the quality of the vegetable, biological soil additive which can prevent the disease and as well as increase the production is confected, comprising two microorganisms, nonmetal mineral, fermented organic fertilizer, industrial waste residue and mineral microelement. The components comprise the weight proportion as follow: 10 percent of trichoderma functional microbial agent, 2 percent of VA arbuscular mycorrhizal fungi, 6 percent of inorganic nonmetal mineral zeolite, 69 percent of fermented organic fertilizer, 4 percent of shell powder, 7 percent of calcium superphosphate and 2 percent urea. The present invention is widely applied to the vegetable planting at the protected field with seriously continuous cropping and successive cropping and has the functions of improving the soil, promoting the crop nutrient absorption, increasing the output and preventing the disease.

The invention discloses a preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and an application thereof, and relates to the preparation and the application of bio-fertilizer. The compound rice dedicated bio-fertilizer aims to solve the problem that rice can not be infected by the arbuscular mycorrhizal fungi under the aquatic condition at present and is difficult to effectively form mutualistic symbiosis, and therefore the rice can not obtain mineral nutrition from an arbuscular mycorrhizal fungi-crop symbiotic system. The method comprises the following steps of: preparing a colonized reinforcer; pre-processing arbuscular mycorrhizal fungi fungicide; and evenly mixing the processed arbuscular mycorrhizal fungi fungicide, rice shoots growth promoterand the colonized reinforce. The compound rice dedicated bio-fertilizer can be prepared into a seedling bed, culturing the mycorrhiza seedling and managing a rice field. According to the compound rice dedicated bio-fertilizer disclosed by the invention, the arbuscular mycorrhizal fungi-crop symbiotic system with a high infection rate and stable performance can be built in the seedling stage of the rice, and the symbiotic system can successfully live through the transplanted field flooding environment. The biological bactericide activity loss problem brought by flooding intimidation can be effectively solved, mineral nutrition is continuously provided for the rice, the growth situation of the rice is improved, the fertilizer application amount in the field is reduced, and the rice production cost can be effectively lowered.

The invention relates to a method for improving artificial breeding survival rate of populus diversifolia by inoculating arbuscular mycorrhizal fungi. The method comprises the steps of: selecting populus diversifolia sprouts for performing pot experiment, arranging a transplanting hole in the pot, spreading sandy soils rich in glomus mosseae spores at the bottom of the hole, slowly rotating the root systems of populus diversifolia seedlings on the sandy soils of glomus mosseae spores before the transplantation, so that the sandy soils are pasted on the root systems of the populus diversifolia seedlings, transplanting the populus diversifolia seedlings into the hole in the pot, watering a dilute Hoagland nutrient solution every time, and observing the survival rate; or enabling populus diversifolia seeds to absorb water and swell and accelerating germination in an incubator, mixing the populus diversifolia seeds with the sandy soils of glomus mosseae spores, spreading the mixture on a sandy layer surface of a culture bed, and paving a layer of sandy soils rich in glomus mosseae spores under the sandy layer of the culture bed in advance, wherein the root systems are to pass through the sandy soil layer rich in glomus mosseae spores, so that the root systems can be infected. Compared with a control group, the populus diversifolia seedlings obtained by the method are obviously different from the populus diversifolia seedlings in the control group in the growing speed and survival time, particularly in survival rate.

The invention relates to a method for plant restoring soil of arsenic contamination, which is realized through inoculating arbuscular mycorrhizal fungi to centipedefern, a kind of pteridophyte, thus producing fungus root, the method comprises the steps of, (1) collecting raw pteridophyte material, obtaining spores of pteridophytes, cultivating young plant sprout, (2) producing fungus roots from pteridophyte, (3) transplanting the adult strain of young sprouts to arsenic contaminated soil. The invention can be applied to aid the arsenic super rich centipedefern to adapt to arsenic contamination environment.

The invention discloses a method for carrying out industrialized production on an arbuscular mycorrhizal fungi agent, and relates to a production method of arbuscular mycorrhizal fungi. The method comprises the following steps: 1. preparing and treating the arbuscular mycorrhizal fungi agent; 2. carrying out seed treatment on hosts; 3. carrying out breeding; and 4. managing. The method disclosed by the invention has the advantages that the implementation of production just needs eight weeks, the number of effective spores of arbuscular mycorrhizal fungi in each gram of the obtained arbuscular mycorrhizal fungi agent is 120-150, the production cycle is short, and the yield is high. The method disclosed by the invention can be applied to the field of agriculture, gardens, and flowers.

Mycorrhizal and rhizobial associations represent the two most important symbiotic relationships between higher plants and microorganisms, providing access to otherwise limiting supplies of phosphate and nitrogen, respectively. Although many higher plants are able to establish a symbiotic relationship with arbuscular mycorrhizal fungi, legumes are unusual among plants because they also form associations with nitrogen fixing soil bacteria called rhizobia. This symbiosis requires the production of bacterial signals, “Nod factors” that trigger several key developmental responses in the host plant (Dénarié et al., 1996). The DMI1 gene of the model legume M. truncatula plays a major role both in the early steps of Nod factor signaling and in the establishment of mycorrhizal symbiosis. Dmi1 mutants do not exhibit many of the early responses to Nod factors and are incapable of forming nitrogen fixing root nodules. Here we describe the cloning and preliminary characterization of DMI1. The DMI1 gene encodes a novel protein with low global similarity to ligand-gated cation channels of archaea. The protein is highly conserved in angiosperms and ancestral to land plants. Interestingly a putative A. thaliana DMI1 orthologous gene is expressed in roots. As A. thaliana is unable to establish a mycorrhizal symbiosis, this finding suggests that DMI1 may also exhibit a function that is independent of symbiotic interactions.

The invention relates to a method for promoting growth of sweet sorghum in a saline-alkali land with arbuscular mycorrhizal fungi and an organic fertilizer, sweet sorghum seeds are sown in the saline-alkali land, the arbuscular mycorrhizal fungi are inoculated and the organic fertilizer is applied simultaneously, and conventional field management is performed in the growing process of the sweet sorghum until harvesting. According to the method, the buffering performance and adsorption capacity of the soil can be enhanced by the aid of the actions of adsorption, complexing, reduction, volatilizing and the like of the organic fertilizer, the toxic action of saline ions is reduced, and the plant growth is promoted; the effectiveness of the saline ions is reduced by the aid of the chelation, the hypha immobilization function and the like of the arbuscular mycorrhizal fungi, the plant tolerance is improved in various ways of improving the plant nutriture, changing the rhizosphere physical and chemical properties, changing the root morphology, adjusting the expression of some salt-resistant genes and the synthesis of related proteins and the like, and the arbuscular mycorrhizal fungi and the organic fertilizer are applied simultaneously, so that saline-alkali stress can be relieved synergistically.

The invention discloses a Jatropha curcas mycorrhizal fungi compound fertilizer, which comprises one or more arbuscular mycorrhizal fungi containing glomus and gigaspora that are served as the mycorrhizal fungi. The compound fertilizer consists of 20-30 percent of the mycorrhizal fungi, 1-12 percent of phosphate fertilizer, 2-18 percent of potassium fertilizer, 8-15 percent of nitrogenous fertilizer, 2-3 percent of zinc sulfate and 20-50 percent of humus soil. The compound fertilizer of the invention can improve the absorption ratio of Jatropha curcas root absorbing soil nutrition, meet the growth, development and fruit bearing of the Jatropha curcas, improve soil ecological environment and increase seed yield.

The invention relates to a method for promoting growth of sweet sorghum in saline-alkali soil through arbuscular mycorrhizal fungi. The method includes the steps of preparing and applying an arbuscular mycorrhizal fungi agent. When the arbuscular mycorrhizal fungi agent is prepared, a mixture of farmland soil and organic fertilizer serves as a substrate, and the organic fertilizer and decomposed products of the organic fertilizer can be used as a fungi protecting agent to have a buffering effect on saline ions in soil around the arbuscular mycorrhizal fungi in the early period when the fungi agent is applied to the saline-alkali soil so as to help the arbuscular mycorrhizal fungi to resist saline-alkali stress; the symbiotic relationship is smoothly formed between the arbuscular mycorrhizal fungi and the sweet sorghum, the mineral nutrition of the sweet sorghum is remarkably improved, the sweet sorghum can be helped to resist saline-alkali stress, and the growth of the sweet sorghum is promoted.

The invention provides an arbuscular mycorrhizal fungus for repairing atrazine-contaminated soil and relates to a microorganism, in particular to an arbuscular mycorrhizal fungus, solving the problem that the effect of the existing method adopting organisms to repair the atrazine-contaminated soil is unsatisfactory. Moses sacculus mildew HDSF1 is preserved in the Common Microorganism Center of China Committee for Culture Collection of Microorganisms, with the preservation number of CGMCC No. 3012. After the Moses sacculus mildew HDSF1 is inoculated in the soil contaminated by atrazine, the growing trend of a crop plant sown on the soil is good, and the biomass is obviously higher than that not inoculated with the repairing arbuscular mycorrhizal fungus of the invention; and the residual quantity of the atrazine in the soil is greatly reduced. In the arbuscular mycorrhizal fungus for repairing the atrazine-contaminated soil, the Moses sacculus mildew HDSF1 is not influenced by indigenous microorganism contamination in the repaired soil, and the anti-interference property is strong; and under the condition that the concentration of the atrazine in the soil is lower, favorable biological degradability can still be kept, and the biological activity is kept.

The invention discloses a method for improving quality of fruits of citrus. The method for improving the quality of the fruits of the citrus comprises the following steps: firstly, preparing arbuscular mycorrhiza fungal inoculant; secondly, uniformly mixing the prepared arbuscular mycorrhiza fungal inoculant obtained in the step one with garden soil in volume ratio of 1:(4-5), so that mixed matrix is obtained; thirdly, uniformly applying 500-600g of mixed matrix in an annular region 40-50cm inward water drip of crown of the citrus. The method for improving the quality of the fruits of the citrus has the advantages that growth of the citrus is promoted, and the quality of the fruits is improved; and the method for improving the quality of the fruits of the citrus is simple in flow and easy in operation, does not pollute or destroy soil, can provide good technical guidance for sustainable utilization of the soil and has a large-scale popularization application prospect.