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Method for inducing drought resistance of cucumber by arbuscular mycorrhizal fungi

A technology of arbuscular mycorrhizal fungi, arbuscular mycorrhizae, applied in the directions of fertilization methods, botanical equipment and methods, horticulture, etc.

Inactive Publication Date: 2014-05-21
ANHUI UNIV OF SCI & TECH
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AI Technical Summary

Problems solved by technology

There are still few reports on using mycorrhizal technology to improve the drought resistance and drought tolerance of tree species suitable for afforestation in rocky mountainous areas

Method used

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Experimental program
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Embodiment Construction

[0012] (1) Inoculate the initial arbuscular mycorrhizal fungus alone for propagation, and the propagule spore density of the expanded arbuscular mycorrhizal> 30 per gram of dry soil, the initial arbuscular mycorrhizal fungal inoculant refers to G. mosesii GM (BGCNM01A, BGCHEB07B, about 300 spores / 20g), and G. terrestrial GV (BGCGD01C, about 1000 spores / 20g), Glomus intraroot GI (BGCBJ09), the arbuscular mycorrhizal fungal inoculation used for the two mixed inoculations is made by mixing the above three fungi separately after propagation, and the mixing ratio is 0.5-1:1:1. In terms of weight ratio; the initial arbuscular mycorrhizal fungal inoculant multiplication: mix river sand and soil at a ratio of 3:1, using clover or corn as host plants; pass the river sand soil mixture through a 1mm sieve, and steam at 121°C. Bacteria or dry heat sterilization at 160~170℃ for 2h, add 0.005~0.01% urea and 0.02~0.04% superphosphate, inoculate 3%~5% initial arbuscular mycorrhizal fungal inocu...

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Abstract

A method for inducing the drought resistance of cucumbers by arbuscular mycorrhizal fungi comprises the following steps: carrying out individual inoculation and propagation expanding initial arbuscular mycorrhizal fungi inoculants; cultivating cucumber seedlings through mycorrhization of arbuscular mycorrhizal fungi inoculants, wherein inoculation media adopts a mixture of a Hoagland nutrient solution and perlite; inducing drought resistance: after the seedlings are grown for 3 months, transferring the seedlings in black seedling raising bags with the specification of 25 cm*25 cm, filling 3.0 kg of sterilization soil in each black seedling raising bag, applying 80 mg / kg of total phosphorus and 100 mg / kg of total nitrogen in each basin, and keeping the relative water content of the soil to be about 40% of the field capacity under moderate drought; sufficiently watering before processing to enable the seedlings to be in the natural drought, carrying out the water control processing when the natural drought reaches the preset relative water content in processing, using a weighing water supplementing method to keep the all repeated soil water content in each-time processing to be within the test required range during the water control period, setting the regulating time of the water content to be 8: 00 Am each day, and supplementing water lost on a previous day.

Description

Technical field [0001] The invention relates to a method for inducing resistance, in particular to a method for inducing drought resistance of cucumber by arbuscular mycorrhizal fungi. Background technique [0002] Arbuscular mycorrhiza (Arbuscular mycorrhiza, AM) is a combination of mycorrhizal fungi in the soil and the root system of higher plants, and it is a common plant symbiosis phenomenon that exists widely in nature. A large number of studies have found that mycorrhiza can promote plant growth, enhance plant resistance, improve plant water and nutrient absorption capacity, and improve soil capabilities. At present, the restoration and reconstruction of degraded ecosystems has attracted much attention at home and abroad, and has become one of the research hotspots in the field of modern ecology. AM inoculation technology has opened up an important way for mycorrhizal seedling cultivation and afforestation to effectively solve the vegetation restoration of degraded ecosyst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00A01C1/02A01C21/00
Inventor 许亚峰袁力汪浩然陈艳花徐华超谢淮北
Owner ANHUI UNIV OF SCI & TECH
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