517 results about "Mycorrhiza" patented technology

Compositions, methods and business applications of using new and recycled cardboard infused with a plurality of saprophytic (including endophytic) and mycorrhizal fungi matched with seeds of plants (including trees, vegetables, herbs and grasses) whereby the cardboard can be sprouted by end-users to start ecosystems. Such containers may have carbon-credit value for companies and consumers when planted and grown as a carbon sink or carbon offset for the photosynthetic and mycelial sequestration of carbon dioxide. The relative weight of the Life Box's added seeds and spores does not significantly affect the total weight of the infused cardboard, thus not increasing transportation costs.

The invention relates to a combined restoring method of polycyclic aromatic hydrocarbon contaminated soil, comprising the following restoring steps of: planting lucerne as perennial leguminous plants in the polycyclic aromatic hydrocarbon contaminated soil; inoculating a mixed bacterial inoculum comprising arbuscular mycorrhizal fungi, bacillus and flavobacterium; and meanwhile, adding rhamnolipid as a biosurfactant. By rhizosphere effect generated by plant root exudates, the number and the metabolic activity of soil microorganisms are increased; and by inoculating the arbuscular mycorrhizal fungi, the plant growth is promoted, and the root exudates are increased, thereby increasing the root accumulation of organic pollutants and the number of root soil microorganisms. The inoculated high-efficiency polycyclic aromatic hydrocarbon degrading bacteria use plant root secretions as a carbon source and an energy source and can quickly grow and propagate. The polycyclic aromatic hydrocarbon is stripped from soil grains by the biosurfactant, and the bioavailability and the degradability of the organic pollutants are increased.

The invention relates to a coal gangue-fly ash-sludge substrate and a mycorrhiza technology for restoring ecology of a mining area. The invention belongs to the technical field of environmental protection, and relates to an ecology restoring technology, in particular to a method for restoring the ecology of an abandoned coal mine area. The method for restoring the abandoned coal mine area by the mycorrhiza technology of the invention adopts a composite substrate prepared by mixing fly ash, coal gangue and sludge, and comprises the following steps: a, preparing an inoculant; b, preparing a basic plant material; c, carrying out inoculating mycorrhiza treatment on the plant by the inoculant obtained in the step a; d, analyzing the physicochemical property of the abandoned coal mine area, and carrying out mixed treatment to form the composite substrate; and e, sowing seeds on the composite substrate surface layer of the treated abandoned coal mine area. The method of the invention can improve the adaptability of plants to the complex adverse environment of the abandoned coal mine area, wherein the adaptability comprises stronger nutrient acquisition capability and cold resistance, thereby fully utilizing waste and sludge in the mining area, and solving the source problem of other soil for melioration in the mining area.

The invention is suitable for the technical field of recycling organic wastes, and discloses a flora for recycling treatment of organic wastes. The flora for recycling treatment of organic wastes is efficiently compounded from a variety of bacteria such as bacillus subtilis, aspergillus niger, saccharomycete and soil flora, wherein the soil flora is extracted from the natural world and comprises three or more of bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, mycorrhiza fungi, azotobacter vinelandii, photosynthetic bacteria, bacillus coagulans, aspergillus oryzae, and paecilomyces lilacinus. According to the flora for recycling treatment of organic wastes, disclosed by the invention, by virtue of mutual promotion, mutual coordination and combined action of a variety of beneficial microorganisms, the organic wastes are recycled, environmental pollution is reduced, and wastes are changed into valuables; and the flora for recycling treatment of organic wastes can be used for producing industrial fermentation products and improving the health and growth conditions of animals, and can also improve the current situation of serious soil hardening at present and improve soil quality, thereby promoting the rapid development of agricultural economics.

A soil improver and method for making the same. The soil improver, in one example, comprises a nitrogen source, a surfactant, a multi-mineral, and a microbial blend. The microbial blend comprises at least one bacterium, at least one fungus, and at least one mycorrhiza. The mycorrhizae mine water and nutrients for plant roots in exchange for food. Further the mycorrhizae greatly expands the effective root zone of the host plant. The soil improver is made by mixing the dry ingredients. Thereafter the microbial blend is mixed with the dry ingredients.

The invention provides a method for synthesizing Chinese truffles and mycorhiza of China pinus montana. The steps related are as follows: filtration of China pinus montana seeds and truffle seed entities; culture of aseptic seedlings; preparation of microbial inoculum; arrangement, optimal selection and inoculation of culture substrates; culture of mycorrhizal seedlings; anatomization of mycorhiza forms; detection and confirmation of molecules and transplanting culture, and finally, the culture of truffles is realized.

The invention discloses a reproduction method of Chinese cymbidium seedlings, belonging to an orchid seedling reproduction technology, in particular to a reproduction technology for raising seedlings by tissue culture and mycorrhizal fungi intergrowth culture. In the reproduction method of the Chinese cymbidium seedlings, seedling acquisition mainly comprises the steps of seed germination, protocorm successive proliferation and rhizogenesis culture. The reproduction method of the Chinese cymbidium seedlings is characterized by comprising the following steps: carrying out hardening on rooted seedlings and the process of mycorhiza on mycorhiza fungi after the seedlings are obtained. By adopting the mycorhiza culture technology of sterile germination and mycorhiza fungi intergrowth of Chinese cymbidium seeds, the invention has the advantages that excellent variety can be bred, excellent Chinese cymbidium plants can be selected from the seedlings, the Chinese cymbidium breeding speed can be rapidly improved and the defects that pure tissue culture seedlings grow slowly, have long period and even do not bloom can be overcome, thus the invention has an important utilization value on large-area industrial reproduction for the Chinese cymbidium.

The invention relates to a method for using fungus root fungi in the large cultivation of gold thread lotus, wherein used four fungus root fungis are small mushroom pieces, as Mycena dendrobii, Mycena osmundicol, Mycena orchidicola, and Mycena anoectochila. And the method comprises that first cultivating fungus root fungi and gold thread lotus; when transplating the gold thread lotus into large field, adding fungus root fungi and gold thread lotus to be planted together. The invention can reduce the grow period of gold thread lotus, improve yield and the content of effective component.

The invention relates to a method for producing protective vegetable fungus root sprout, comprising the following steps: 1) planting susceptive crop after inoculating the bush AMF for sterilized organic soil or soil, taking the strand roots of plant or fungus root spore as inoculating bacteria; 2) sterilizing seedling soil or medium; 3) loading the sterilized soil into dish, spraying the inoculating bacteria onto soil, 4) sowing on the dish, then covering with sterilized soil for seedling in greenhouse. The invention is characterized by the low cost, good popularity and simple operation. The bacteria can on one side increase the usage of nutrient and water and fertilizer under organic cultivating condition, which results in productivity and quality improvement of vegetable; on the other side can strengthen the disease and drought resistance, reduce disease spread through soil and improve vegetable root condition.

The invention relates to a blueberry mycorrhizal fungi (coprinus micaceus) and a separation method and application thereof, belongs to the technical field of microbiology, and mainly relates to a mycorrhizal fungi which separated from a Changbai Mountain wild cowberry root system for blueberry inoculation and application of the mycorrhizal fungi at promoting the growth of blueberry and improving the yield of the blueberry. The blueberry mycorrhizal fungi is preserved in the Chinese General Microbiological Culture Collection Center, and the classification name is Coprinus micaceus. By the application of the mycorrhizal fungi, the growth of the blueberry root system can be promoted. The invention has the advantages that the yield, fruit setting rate and insect damage prevention function can be improved, and the like.

The invention relates to a method for using soybean root nodule and endotrophic mycorrhiza to prevent soybean root disease. Wherein, the inventive bacteria seed has been stored in national microbe storage manager center, whose storage number is sinorhizobium sp; the seed L396 storage number is CGMCC No. 1851; the bacillus subtilis seed Snb2 storage number is CGMCC No.1850. And the invention uses general liquid to ferment and cultivate, uses itself or metabolite as effective active component; mixing the ferment solutions at different ratios, with effective restrain function on soybean cyst nematization and root pathogenic fungi; the density ratio between Snb2 and L392 is 8:3, and the dead ratio of young bacteria can reach 98.92%, and the relative restrain rate on cyst cultivation can reach 99.66%; the invention prepares the metabolite into water agent, to reach 78.6% prevent rate on the soybean cyst nematization. The inventive nodule bacteria can fix nitrogen and accelerate growth.

The invention discloses a method for optimizing nursery-grown plant cultivation by using ectomycorrhizal fungi. The method comprises the main process including the following steps: production of an ectomycorrhizal fungi agent, cultivation of tree seedlings, inoculation with the ectomycorrhizal fungi, and nutrition management of the tree seedlings after inoculation. In actual use, a pre-experiment can be performed according to different tree species, and a best ectomycorrhizal fungi strain is selected. 6 kinds of ectomycorrhizal fungi are adopted as materials to inoculate root systems of the tree seedlings, and the growth amount and the transplanting survival rate of the tree seedlings are significantly improved.

The present invention relates to an application technique of mycorrhizal fungi in cultivation of anoectochilus formosanus. It is characterized by that the used mycena-generic four fungi respectively are mycena dendrobii, mycena osmundicola, mycena orchidicola and mycena anoectochila. First, the mycorrhizal fungi and seedling of anoectochilus formosanus are respectively cultured, then they are symbiotically cultivated. The adoption of said invented technique can greatly shorten the growth period of anoectochilus formosanus, and can raise its yield and effective component content.

The invention discloses a cottage breeding method of sabina przewalskii, comprising the following steps: manufacture of a cottage seedling bed: manufacturing a seedling bed on the seedling culturing ground in a greenhouse in the spring of the current year; and covering fine sand and mycorrhiza soil as cottage matrix; strip picking: in the middle of May to June, selecting healthy, strong, pest and disease-and-damage-free sabina przewalskiiseedlings at the age of 3-5 with good growth and development for picking strips; and cutting processing: placing the cutting into clear water for soaking, drying rapidly, respectively placing into ABT1 rooting powder and potassium permanganate solution for respectively soaking for one time and then carrying out cottage; digging holes on the cottage seedling bed and obliquely cutting, and compacting the cutting hole; leading the cutting bed and the cutting to be fully contacted; spraying with carbendazim or Tuzet and copper abietate for once every half a month, thus preventing root rot of sabina przewalskii seedlings. The method of the invention greatly improves propagation coefficient, can shorten seeding culturing period, has high cottage seedling culturing survival rate, and can enable the cultured nursery stock to keep excellent characters of parent trees.

Disclosed is a process for comprehensive treatment of discarded metal tailings, wherein fungus root technology is employed to recover the metal tailing vegetation, the process comprises the steps of, (1) preparing the seeding agent, (2) preparing the basic plant material, (3) carrying out seeding bacteriorhiza treatment to plants by using the seeding agent obtained in (1), (4) conducting physical and chemical analysis to the metal tailing and related treatment, (5) sowing seeds or replanting young sprouts on the skin layer of the treated metal tailing, (6) curing the young sprouts.

The invention relates to a method for producing the edible fungus mycorrhizal seedling of a mycorhiza. The method is characterized by comprising the following steps: the mycelium of a target mycorrhizal fungus and the mycorhiza of a non-competitive mixed fungus are synthesized into a culture medium, are uniformly mixed, are spread in a container and are cultured under a condition which is suitable for the growth of hypha, wherein the surface of the container is disinfected by a potassium permanganate solution or sterilized under high temperature and high pressure, and the lower part of the container is spread with a layer of non-competitive mixed fungi or media sterilized under high temperature and high pressure; and a sterile germinated host plant seed of which the surface is disinfected and the culture medium culturing the target hypha are mixed and cultured under a proper ecological condition until the mycorhiza of a tree seedling root is formed. The synthesis experiment of the edible fungus mycorrhizal seedlings of various mycorhiza shows that the method has simple and effective operation and high efficiency, is very suitable for popularization and application in mass production, can be used for producing mycorrhizal seedlings at any time in one year and has obvious economic benefits.

The invention discloses application of a method for smearing and transforming agrobacterium rhizogene-mediated hypocotyl to soybean transformation. The method for smearing and transforming agrobacterium rhizogen-mediated hypocotyl to is applied to soybeans for the first time, and hairy root transformation of soybean roots is successfully carried out. In this invention, the sprouting time of seeds and the concentration of screening agents are optimized, therefore not only increasing the quantity of transgenic hairy roots induced by each soybean explant, but also obviously enhancing the transformation frequency and growth conditions of hairy roots. A screening marker weed killer usually used for soybean transformation is successfully used for obtaining soybean compound plants in aseptic conditions, wherein each soybean compound plant has a transgenic root and a non-transgenic over-ground part and can be used as the ideal material for studying genes relative to the form of root systems, the symbiotic nitrogen fixation of roots and the formation of fungus roots.

The invention relates to a method for producing dendrobium officinale tissue culture mycorrhizal seedlings, which comprises the following steps of: (1) culturing mycorrhizal fungi by using a solid or liquid culture medium, wherein the mycorrhizal fungi are mycorrhizal fungi LP1, namely Chaetomium sp.; (2) culturing mycorrhizal bacteria, wherein the mycorrhizal bacteria DP6 are Azotobacter sp.; (3) culturing the dendrobium officinale tissue culture seedlings; and (4) carrying out water culturing and training on the tissue culture seedlings. With the method for producing the dendrobium officinale tissue culture mycorrhizal seedlings, provided by the invention, not only the growth of the dendrobium officinale tissue culture seedlings is promoted, but also the tissue culture seedlings are obviously thickened. After transplantation, the survival rate can reach more than 95%, and the tissue culture seedlings have high growth rate, great bud tillering rate, rapid growth rate and strong stress resistance. Compared with the control group of dendrobium officinale seedlings which are not inoculated to mycorrhizal fungi and bacteria to perform tissue culture, the dendrobium officinale seedlings inoculated to mycorrhizal fungi and bacteria have more prosperous root systems, obviously inflated plant internodes and high fresh weight increased by 10-20%.

The invention provides a method for improving the plant-based recovery efficiency of dichlorodiphenyl trichloroethane (DDT)-polluted soil. The method is to promote mycorrhiza of clover and add chemical surfactant to recover the DDT-polluted soil with the joint function of uromyces striatus schroter and the surfactant. The steps of the method include that an inoculant layer is inoculated in a culture medium; mycorrhiza is carried out to the clover and that the sprouted clover is planted in the DDT-polluted soil and the surfactant TritonX-100 is added to the soil. The joint effect of the mycorrhiza and the surfactant can remarkably improve the resistance of plants to the DDT, reduce the concentration of the DDT in the soil and have evident plant-based recovery efficiency of the DDT-polluted soil. The method of the invention can be used for reference for the recovery of the soil polluted by other organic matters.

The invention discloses a method for screening tulasnella fungi and establishing a symbiotic system for promoting seed germination of cymbidium mannii and cattleya hybrida. A Tcs1 bacterial strain isadopted and establishes a symbiotic relation together with one or more orchid seeds including cymbidium mannii seeds, cattleya hybrid YK seeds and cattleya hybrid YD seeds to promote germination of orchid seeds. A symbiotic germination test is carried out on an artificial medium through adoption of tulasnella fungi and orchid seeds, and is compared with a control group of orchid seeds with no fungi inoculated to screen mycorrhizal fungi capable of widely efficiently promoting seed germination and to screen appropriate symbiotic mediums. Efficient symbiotic fungi for a germination stage of orchid seeds are obtained, the germination rate of orchid seeds is improved via symbiotic germination culture technique, and a symbiotic system of orchid and mycorrhizal fungi is established.