214 results about "Mycorrhiza" patented technology

The invention discloses a method for restoring soil heavy metal pollution by coupling soybean urease with a charcoal cementing agent, and relates to the technical field of soil heavy metal pollution restoration, and the method comprises the following steps: dynamic microbiome regulation and control: introducing photosensitive protein into soil functional bacteria; the acyl homoserine lactone analogue is synthesized and applied to soil, and is competitively combined with a microbial quorum sensing receptor to block an inhibitor to secrete a signal channel; according to the method, microbial urease secretion is regulated and controlled through optogenetics, an inhibitor signal is blocked through quorum sensing interference, and a microbiome is precisely regulated and controlled; the pH-responsive nanocapsule releases an inhibitor in a targeted manner, and the magnetic-responsive nanochain dynamically captures interfering bacteria, so that the limitation of passive adsorption is broken through; the gene editing plant releases coumarin to inhibit fungal metabolism, and the engineering mycorrhizal fungi physically shields an inhibitor to construct cross-biosphere cooperative defense; the urease activity and the heavy metal removal rate are remarkably improved, meanwhile, the soil microbial diversity is maintained, and the soil specificity interference problem is solved.

The invention provides a special package compound fertilizer for peppers and a topdressing method thereof. The special package compound fertilizer comprises a nitrogen fertilizer, a phosphate fertilizer, a potash fertilizer, a calcium fertilizer, a magnesium fertilizer, a boron fertilizer, a microbial compound fertilizer and an amino acid fertilizer. The microbial compound fertilizer is prepared from a mixed arbuscular mycorrhizal fungal agent, a zinc fertilizer and manure, the mixed arbuscular mycorrhizal fungal agent is Glomus fasciatus Vermann and sclerosporum Dusii, topdressing is started according to the mass ratio of nitrate ions to ammonium ions being 1: (2-3) 10 days after the pepper is transplanted, and topdressing is carried out for 6 times in total. According to the chili package compound fertilizer, the chili nutrient absorption rule and the nutrient utilization efficiency are taken as the fertilization starting point, the nitrogen-phosphorus-potassium fertilizer is taken as the main body, and medium trace elements with large demand quantity for chili are added, so that the chili yield can be remarkably increased, the fruit quality can be improved, and the chili package compound fertilizer is suitable for being popularized and used in chili planting areas.

A method of formulating novel humic material is disclosed comprising mixing one or more portions of Dimethylphenylpiperazinium (DMPP) with one or more portions of N-(N-butyl) thiophosphoric triamide (NBPT) with one or more portions of Isobutylidene-diurea (IBDU) with one or more portions of Polyaspartic Acid with one or more portions of Chitosan and a portion of Mycorrhizae and Rhizobia to form a portion of biostimulant material; obtaining a portion of seaweed harvest and crushing and drying said portion of seaweed to form a portion of seaweed powder; Obtaining a portion of leonardite and crushing said portion of leonardite to form a portion of humic raw material; mixing one or more portion of animal manure with one or more portion of stover with one or more portion of organic waste to form a portion of compositing mix and composting said compositing mix to form a portion of composted material; obtaining a portion of plant waste and subjecting said portion of plant waste through an anaerobic combustion to form a portion of bio char; mixing said portion of bio char with said portion of composted material with said portion of humic material to form a portion of humic processed material; adding a portion of artificial taggant to said humic processed material to form tagged humic material; mixing said tagged humic material with said portion of biostimulant material to form a portion of biostimulant humic material; adding a taggant to said portion of biostimulant humic material to form a portion of tagged biostimulant humic material; mixing one or more portion of phosphorus with a portion of potassium and a portion of nitrogen and a portion of trace minerals to form portion of raw fertilizer; mixing said portion of raw fertilizer with said portion of tagged biostimulant humic material to form a portion of tagged fertilized biostimulant humic material.

The invention discloses an arbuscular mycorrhizal fungus hypha collection method, and particularly relates to the technical field of hypha collection, and the arbuscular mycorrhizal fungus hypha collection method comprises the following steps: S01, soaking quartz sand in 0.1 mol / L diluted hydrochloric acid, cleaning with tap water until the quartz sand is neutral, and drying with a drying oven; s02, cutting a nylon net with the aperture of 10-20 microns, and making the nylon net into a nylon net bag by using a sealing machine; s03, 70g of quartz sand and a proper amount of slow-release fertilizer are put into a nylon mesh bag, and then the nylon mesh bag is subjected to opening binding through a sealing machine to be made into a hypha collecting bag. The device is suitable for potting and field experiments, a large number of mycelia can be efficiently collected, and the root system of a plant can be prevented from entering the mycelia bag.

The invention discloses a slope ecological restoration base material based on multistage synergistic solidification and a layered spray-seeding method, and belongs to the technical field of geotechnical engineering and ecological restoration. The invention provides a three-layer collaborative base material system and a dynamic construction process. In the base material, the bottom layer takes modified volcanic ash and nano silica sol as cementing materials, so that rock surface permeation and adhesion are enhanced; the middle layer adopts chitosan slow-release particles and gradient moisture content design, so that nutrient stage release and interlayer stress buffering are realized; the surface layer is combined with the 3D honeycomb-shaped biochar carrier and the degradable PLA grid, grass seeds and mycorrhizal fungi are synchronously loaded, and a physical-biological composite anchoring structure is formed. The construction method comprises high-frequency micro-vibration permeation, layered gradient spray-seeding and photosensitive non-woven fabric covering processes, and it is ensured that the base material is resistant to rainstorm scouring and rapid in vegetation covering. The method has both mechanical stability and ecological compatibility, is suitable for repairing the high and steep rock slope, and remarkably reduces the maintenance cost and the environmental pollution risk.

The invention discloses a mycorrhizal fungus substrate beneficial to blueberry cultivation and a mycorrhizal cultivation method in the technical field of crop cultivation. The mycorrhizal fungus substrate comprises the following steps: S1, collection and isolated culture of Guizhou wild azaleaceae and blueberry mycorrhizal fungi; s1-1, collecting root systems and soil around the root systems of different types of azaleaceae plants in different regions; s1-2, separating and culturing dominant wild rhododendron mycorrhizal fungi by adopting a pure culture and high-throughput sequencing technology, carrying out a tieback test by taking blueberries as a material, determining physiological and biochemical indexes of the mycorrhizal fungi and grafted seedlings, and screening excellent mycorrhizal fungi suitable for blueberry symbiosis; s2, developing a reproduction substrate matched with the blueberry mycorrhizal fungi; humus soil, peat soil and organic fertilizer in different proportions are adopted for inoculation test, and a proper inoculation matrix and the optimal mycorrhizal fungi inoculation amount are screened; according to the method, Guizhou wild rhododendron mycorrhizal fungi are utilized to establish an efficient mycorrhiza-blueberry symbiont, so that the survival rate of blueberries is ensured, the production capacity is improved, the ecological blueberry industry is developed, and a degraded ecological system of slope cropland is recovered.

The invention provides inter-plant mycorrhiza nutrient transfer culture equipment which comprises a culture box body and an organic glass plate, an inner cavity of the culture box body is divided into a first culture chamber and a second culture chamber through the organic glass plate, the organic glass plate is a porous glass plate, and a white nylon net is pasted on one side of the porous glass plate. And the white nylon net is divided into a white nylon net with the aperture of 0.45 mu m and a white nylon net with the aperture of 37 mu m. The equipment is small in size, small in occupied area, cheap in material, convenient to assemble and suitable for pot culture experiments of large-batch samples, soil ions are allowed to pass through by means of 0.45 microns, ectogenic hyphae and the soil ions are allowed to pass through by means of a white nylon net of 37 microns, the soil ions, the ectogenic hyphae and root systems are allowed to pass through a nylon-free net, and the soil ions, the ectogenic hyphae and the root systems are allowed to pass through. Compared with the prior art, direct and indirect paths of nutrient transfer between plants can be distinguished and quantified, and the method is simple and easy to implement.

The invention relates to the technical field of agricultural biology, and discloses a microbial agent for preventing and treating fructus aurantii scab and a preparation method of the microbial agent. The microbial agent comprises the following raw materials in percentage by weight: 10-13% of paenibacillus mucilaginosus; 15%-20% of trichoderma sp. 7%-10% of paecilomyces lilacinus; 5%-8% of bacillus pumilus; 7%-9% of mycorrhizal fungi; 5%-8% of streptomyces; 5%-9% of a coptis chinensis extract; 4%-7% of a sophora flavescens extract; 2%-4% of realgar; 1%-3% of a rheum officinale extract; 10%-13% of soybean meal; 5%-8% of a water-soluble fertilizer; 7%-9% of potassium fulvate; 1%-3% of chitosan; 0.5%-1% of trace elements; 3%-8% of humic acid; the raw materials are subjected to microbial flora amplification culture, plant antibacterial component light-shielding mixing and low-temperature mixing to effectively protect the microbial activity of the microbial agent, and step-by-step operation can avoid damage of high temperature and mechanical shearing to microorganisms, so that the microbial activity of the microbial agent is effectively protected. And the loss of microbial active ingredients is reduced by vacuum drying and low-temperature granulation processes.

The invention discloses a growth-promoting rhizobacteria, a growth-promoting rhizobacteria microbial inoculum and application thereof, and belongs to the technical field of microorganisms, the growth-promoting rhizobacteria is Pseudomonas mohnii, the strain number is YQ142, the classification name is Pseudomonas mohnii, and the growth-promoting rhizobacteria is preserved in China General Microbiological Culture Collection Center on April 7, 2025, and the preservation number is CGMCC No.34110. The invention further discloses a preparation method of the growth-promoting rhizobacteria. The growth-promoting rhizobacteria has strong nitrogen fixation and phosphate solubilizing capabilities, can promote the growth of ryegrass, oat and corn, and improves the saline-alkali stress resistance and drought stress resistance of ryegrass.

The invention provides a cultivation method of ornamental small ginger, and relates to the field of plant cultivation, and the method comprises the following steps: tissue culture seedling cultivation: adopting a culture medium comprising a basic culture medium, a plant growth regulator, an organic additive, an antioxidant and trace elements for cultivation to obtain tissue culture seedlings. Performing potting cultivation: transferring the tissue culture seedlings into a potting container on which a mixed substrate is uniformly laid, and performing cultivation, wherein the mixed substrate comprises charcoal particles, modified zeolite particles, a mycorrhizal fungus inoculant, gelatin slow-release particles, fermented soybean meal and graphene modified cellulose; in the cultivation process, irrigation, fertilization and environment regulation and control are carried out regularly. According to the method, high-quality tissue culture seedlings are obtained by using a culture medium with specific components, and growth balance of ginger plants is adjusted and overgrowth of the plants is inhibited by adjusting a planting matrix and regulating and controlling a specific culture method, so that small potted ginger plants for ornamental use are obtained.

The invention relates to the field of soil remediation conditioners, and particularly provides a soil remediation conditioner for durian breeding as well as preparation and application of the soil remediation conditioner. The soil remediation conditioner is prepared from the following components in parts by weight: 35 to 45 parts of organic matter fertilizer, 18 to 22 parts of biochar, 22 to 28 parts of humic acid, 8 to 12 parts of vermiculite, 10 to 14 parts of perlite, 12 to 18 parts of shell powder, 6 to 10 parts of mycorrhizal fungi, EM bacteria and other mixed bacteria, 8 to 12 parts of wormcast, 4 to 6 parts of amino acid chelate solution, 8 to 12 parts of coco coir and 4 to 6 parts of adhesive. The specific surface area of the biochar is 32 m < 2 > / g, and the particle size is 40 meshes; the soil remediation conditioner is applied to durian planting soil remediation, has the effects of improving the physical structure of soil, improving the air permeability and water retention of the soil, increasing the fertility of the soil and providing rich nutrition for breeding and growth of durian seedlings, and has great application potential for the durian industry.

The invention discloses a method for guiding light rare earth surface mine degraded plant community restoration through combination of limonium bicolor, mycorrhizal fungi and tolerant earthworm cast, and the method utilizes a combination mode of limonium bicolor, mycorrhizal fungi and tolerant earthworm cast to guide and construct a plant community-soil fungi-soil ecosystem circulation system. According to the method, the existing plant community of the light rare earth mine is guided to recover by utilizing a combination method of combining dominant plant species with different mycorrhizal fungi and adding tolerance earthworm manure, mycorrhizal fungi and the earthworm manure fertilizer are added in the transplanting process of adult plants, so that the plant survival is promoted, and the stress resistance of light rare earth is enhanced at the same time; plant-mycorrhiza-wormcast fertilizer combinations aiming at different light rare earth elements are screened out, and the whole plant community of the mine is recovered and the content of the light rare earth elements in the soil is reduced through a plant community recovery method.

The invention discloses an ancient tree health state monitoring method based on digital twinning, and relates to the field of ancient tree health monitoring, and the method comprises the steps: collecting point cloud data and texture data of an ancient tree, and constructing an initial three-dimensional model of the ancient tree; a micro ion selective electrode array is deployed in an ancient tree root distribution area, Ca2 and K + ion flow velocity changes are detected through a constant potential rectifier, and the mycorrhizal symbiosis index is calculated in combination with soil temperature and humidity data; uploading the mycorrhizal symbiosis index to a digital twinborn body, and displaying the active state of a root system area in an initial three-dimensional model of the ancient tree in a three-dimensional thermodynamic diagram form; a 16-channel MOX gas sensor electronic nose array is installed on the surface of a trunk of an ancient tree, and volatile organic compound (VOC) concentration data of the ancient tree are collected. According to the invention, a micro electrode array with a 45-degree inclination angle is adopted to non-destructively monitor the ion flow velocity of a root system through the mycorrhizal symbiosis index detection unit, and a three-dimensional thermodynamic diagram is generated in combination with an inverse distance weighted interpolation algorithm, so that dynamic visualization of underground mycorrhizal activity is realized.

The invention relates to the technical field of microorganisms, in particular to a strain (Pezicula ericae) XA4 and application of the strain (Pezicula ericae) XA4. The invention provides a strain (Pezicula ericae) XA4 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on June 26, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41350. The invention also provides a method for preparing the strain (Pezicula ericae) XA4. The XA4 provided by the invention is used for carrying out tie-back on sterile cultured rhododendron dauricum seedlings, and trypan blue dyeing detection shows that mycorrhizal fungi can be successfully subjected to tie-back. Compared with the non-inoculated strain, the inoculated XA4 has the most obvious growth promoting effect on the rhododendron dauricum. The plant height, the leaf length, the leaf width, the root length, the biomass, the chlorophyll content, the net photosynthetic rate, the stomatal conductance, the intercellular CO2 concentration, the transpiration rate and the root activity of the strain are all improved compared with those of the strain without inoculation.

The invention discloses a remediation method for garden contaminated soil, and belongs to the technical field of garden soil remediation, the method comprises the following steps: regulating the pH value of soil to activate heavy metal contaminated soil, then applying a remediation agent to fix heavy metal ions in the soil, and then sowing hyperaccumulator inoculated with arbuscular mycorrhizal fungi. Heavy metal ions in the soil are fixed by applying a repairing agent, so that the hyperaccumulator can well germinate and grow without being stressed by the heavy metal ions, and further more heavy metal ions in the soil can be absorbed and enriched; on one hand, the inoculated arbuscular mycorrhizal fungi can secrete organic acid to activate and release the heavy metal ions fixed by the repairing agent, so that the heavy metal ions are gradually absorbed and enriched by the plants, and on the other hand, the absorption efficiency of the plants on the heavy metal ions can be improved; the hyperaccumulator can well grow in the heavy metal contaminated soil, so that heavy metal ions in the soil are effectively absorbed and removed, and the heavy metal contaminated garden soil is efficiently repaired.

The invention discloses a mycorrhizal seedling culture container convenient for experimental observation. The nutrition cup is composed of a nutrition cup 1, a nutrition cup door 2, a nutrition cup door handle 3, water leakage holes 4, a black light-proof plastic box 5, a plastic plate 6 with holes, a plastic plate door 7 and a plastic plate door handle 8. The nutrition cups 1 are made of transparent plastic materials so that the growth condition of mycorrhiza can be observed at any time, water leakage holes 4 are formed in the cup bottom of each nutrition cup, an opening is formed in the cup wall, and a small door 2 capable of being opened and closed is arranged at the opening so that plant lateral roots can be conveniently taken for experimental observation. The nutrition cup 1 is placed through eight circular openings in the plastic plate 6 with holes, a rectangular opening in the middle of the plastic plate 6 is used for observing watering conditions, and a small door 7 capable of being opened and closed is arranged at the opening. The seedling culture container is placed in the black light-proof plastic box 5 in the mycorrhizal seedling culture process so as to simulate the dark environment in the soil. The novel mycorrhizal seedling culture container is suitable for observing the growth condition of mycorrhiza at any time in the mycorrhizal seedling culture process, sampling the root system of the mycorrhizal seedling at any time without damaging the whole seedling, and is beneficial to scientific research of the mycorrhizal seedling.

The invention discloses a mucilaginosa N15 and a method for promoting the germination of dendrobium huoshanense seeds. The mucilaginosa N15 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on October 27, 2025, and the preservation number of the strain is CGMCC No: 42290. The invention further discloses a method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15 and the method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15. The strain can be prepared into a high-activity microbial agent after activation and amplification culture, and the high-activity microbial agent is mixed with dendrobium huoshanense seeds and then sown in a greenhouse, so that the seed germination efficiency can be remarkably improved. By applying the method, the germination rate of the dendrobium huoshanense seeds can be increased to 60% or above, the mycorrhiza infection rate reaches 80% or above, the comprehensive cost is reduced by 30%, the seedling transplanting survival rate reaches 90% or above, and the seedling raising period is shortened to 12-15 months. The method is easy and convenient to operate, low in cost, high in transplanting survival rate and suitable for large-scale seedling raising and resource protection of dendrobium huoshanense, and has important economic and ecological values.

The invention belongs to the technical field of microorganism application, and particularly relates to a method for improving the growth and drought tolerance of camellia oleifera seedlings by using a microbial agent. The microbial inoculant is composed of a mycorrhizal inoculant and an European Stephania spp. Inoculant, the mycorrhizal inoculant is obtained by propagating a root soil mixture of the root of wild camellia oleifera, the European Stephania spp. Is Peziula ericae PE-1 obtained from the root system of wild camellia oleifera by adopting an endophytic fungus separation method, and the preservation number of the European Stephania spp. Is GDMCC NO: 64597. The method comprises the following steps: firstly, inoculating mycorrhizal inoculant at the roots of the camellia oleifera seedlings to promote the camellia oleifera seedlings to obtain the ability of rapid growth, and secondly, 15 days after the mycorrhizal inoculant is inoculated, irrigating the camellia oleifera seedlings with Eura petiole-free inoculant for 3-5 times to promote the camellia oleifera seedlings to obtain the ability of drought resistance. The method can well help the camellia oleifera to grow and resist drought stress, and is simple and easy to operate, low in investment and good in application prospect.

The present invention belongs to the technical field of soil improvement, and relates to a soil conditioner for desertified vegetation and a preparation method thereof. The raw materials of the soil conditioner include: straw charcoal, humic acid, polyacrylamide, bentonite and composite bacterial agent, wherein the composite bacterial agent is composed of bacillus and mycorrhizal fungi. The prepared soil conditioner can be used for desertified soil to improve the structure of desertified soil, and has the effects of water retention, fertilizer retention and air permeability. In addition, the raw materials of the present invention are easy to obtain, the preparation method is simple, and it is suitable for large-scale production and application.

The invention provides a truffle mycorrhizal seedling cultivation method, and relates to the technical field of plant cultivation. The truffle mycorrhizal seedling cultivation method mainly comprises the following steps: culturing a host seedling, sterilizing truffles, mixing the sterilized truffles with a nutritional agent prepared from anthocyanin and folic acid, pulping together to prepare a suspension, mixing the suspension with a growth agent prepared from indoleacetic acid and tea saponin to prepare a microbial inoculum, inoculating the host seedling with the microbial inoculum, and carrying out seedling hardening cultivation. The method overcomes the defects in the prior art, effectively shortens the mycorrhiza formation time, promotes the growth of mycorrhiza seedlings, ensures the survival rate of subsequent commercial seedling transplantation, comprehensively reduces the cultivation loss, and improves the economic benefits.

The invention relates to the technical field of cultivation of turfs, lawns or analogues, and discloses a vegetation blanket for slope protection and ecological restoration of drought and barren steep slopes and a use method of the vegetation blanket. Adults of the resuscitated plants, plant fibers and a water-retaining agent are prepared into a living resuscitated plant layer together, and the living resuscitated plant layer is subjected to yin cultivation before transplanting, so that normal planting can be carried out regardless of the orientation or existence of root systems, the plant adults are transplanted on a drought and barren abrupt slope in batches, and the survival rate of the plants is increased. A moisturizing and shading layer and a nutrient delayed release layer are adopted to maintain growth of the resuscitated plants, a layer of living soil with a certain water and fertilizer retention effect is formed, and the slope surface is preliminarily protected; after the water and fertilizer conditions on the slope surface are improved to meet the conventional plant growth requirements, coatings on the surfaces of the seeds in the seed delayed germination layer are degraded, so that the seeds germinate and grow at a proper time to realize water and soil conservation.

The invention relates to a big tree inoculation method of Lijiang truffle, and belongs to the technical field of biology. The method comprises the seven steps of environment and inoculated tree requirements, inoculation time selection, preparation before inoculation, strain preparation, big tree inoculation, management after inoculation and truffle harvesting. Compared with a truffle conservation and propagation promotion technology and a mycorrhizal seedling cultivation and planting technology, the period is short, a small amount of truffle sporocarp is found to be formed in winter in the current year of big tree inoculation, the success rate of big tree inoculation reaches up to 80% or above, and the effect is obvious.

The invention discloses a method for controlling the growth condition of strawberry seedlings, and belongs to the technical field of strawberry seedling raising. The method for controlling the growth condition of the strawberry seedlings comprises the steps that illumination uniformity is adjusted, nutrient soil is prepared for strawberry seedling raising, and illumination uniformity adjustment comprises the steps that different numbers of LED lamp tubes are used for irradiating the growing strawberry seedlings, the illumination uniformity of strawberry seedling canopies is improved, and the energy efficiency ratio is increased; the preparation of the nutrient soil comprises the following steps: mixing and stirring the components of the nutrient soil to ensure the uniform distribution of the raw materials, then putting the nutrient soil into a planting pot, watering and wetting for strawberry seedling planting. The nutrient soil comprises the following components in parts by weight: 35-35 parts of leaf mold; 45 parts of the raw materials and 17 parts of vermiculite powder; 23 parts of perlite, and 23 parts of perlite; 30 parts of carbon nitride composite humic acid; 8 parts of the fertilizer and 5 parts of a mixed fertilizer; 7 parts, 1 part of mycorrhizal inoculant; and 3 parts. According to the method, the growth condition of the strawberry seedlings is cooperatively controlled by adjusting the illumination intensity and preparing the nutrient soil, so that the strawberry seedlings are developed in root system, thick and strong in leaf stems and free of plant diseases and insect pests, and the plant height and the leaf area of the strawberry seedlings are obviously increased after the seedling age is 30 days.

The present invention relates to an efficient method for artificially synthesizing Lactarius deliciosus mycorrhiza, which comprises the following steps: (1) Take the mycelium with a size of 1-3 mm at the connection of the stipe and cap of fresh Lactarius deliciosus fruit bodies, place it in a PDA medium, and conduct tissue culture at 20-25 °C for 20-30 days. Then, take the mycelium block and inoculate it onto the PDA medium, and continue to culture at 20-25 °C for 25-40 days. Subsequently, take the mycelium block again and inoculate it into a PDB medium for culturing for 30-50 days to obtain Lactarius deliciosus liquid spawn; (2) Transplant pine seedlings at a density of 6-15 seedlings per basket into a culture basket filled with mycorrhizal substrate, add the Lactarius deliciosus liquid spawn prepared in step (1) to the substrate 3-5 cm deep near the root of each seedling, and mycorrhiza can be formed after culturing for 10-14 days. The present invention scientifically screens the substrate and method for Lactarius deliciosus. By using the optimized medium formula and culture method, the mycelium of Lactarius deliciosus grows faster and the biomass of the obtained Lactarius deliciosus mycelium is larger; inoculating the plant roots with liquid spawn greatly improves the inoculation efficiency; multiple inoculated host plants are cultured in the same culture device, which greatly shortens the mycorrhiza formation time after inoculation and effectively improves the mycorrhiza synthesis rate; reducing the amount of peat soil in the mycorrhizal substrate reduces the proportion of mycorrhiza formed by miscellaneous bacteria and pine roots.

The invention provides a wheat nitrogen fertilizer application reducing and effect increasing method, and belongs to the technical field of biological fertilizers. According to the method, under the background of straw returning to the field, arbuscular mycorrhizal fungi and mushroom residue and excrement decomposed fertilizer are combined as a main body, then peat soil and bentonite are added for wheat cultivation, and application reduction and efficiency improvement of wheat nitrogen fertilizer are achieved; wherein the arbuscular mycorrhizal fungi and the mushroom residue and excrement decomposed fertilizer are combined to promote soil straw degradation and assist nitrogen transfer, the peat soil and the bentonite can improve the water and fertilizer retention capacity of the soil and promote hypha growth and reproduction, available nitrogen in the soil is increased, soil nutrient loss is reduced, and the nutrient absorption capacity is enhanced; further, nitrogen fertilizer absorption and utilization of wheat are improved, and dependence on chemical fertilizer is reduced.

The present invention relates to the field of microbial technology, and particularly to a Cladophialophora sp. CNOCC PP35 and its application in enhancing the photosynthesis of Paphiopedilum purpuratum. The Cladophialophora sp. CNOCC PP35 provided by the present invention is a strain of dark septate endophytic fungus isolated from the mycorrhizae of adult plants of Paphiopedilum purpuratum, with the preservation number of GDMCC NO: 65862. When co-cultured with the axenic seedlings of Paphiopedilum purpuratum, this strain can effectively promote the formation of chlorophyll a and total chlorophyll in the axenic seedlings of Paphiopedilum purpuratum, increasing by 122.35% and 98.42% respectively compared with the axenic control group, and can enhance the photosynthesis of the axenic seedlings of Paphiopedilum purpuratum; this strain can effectively promote the growth of the seedlings of Paphiopedilum purpuratum, with the fresh weight increasing by 127.78% compared with the control group, can realize the mycorrhization of the axenic seedlings of Paphiopedilum purpuratum, and shorten the artificial seedling raising time.

The invention relates to the technical field of crop planting. The invention provides an accurate fertilization and harvesting method for increasing yield and improving quality of sweet corn by nitrate nitrogen and rhizosphere symbiotic AM fungi. According to the technical scheme, the nitrogen and phosphorus compound fertilizer with the optimized proportion and AM fungi are synergistically used, so that accumulation of biomass of the mycorrhizal sweet corn and accumulation of nitrogen elements, phosphorus elements, soluble sugar content and amino acid content in sweet corn kernels are effectively promoted, and then the yield of the sweet corn and the kernel quality are improved; the utilization rate of nitrogen and the utilization rate of phosphorus are increased by 29.54% and 19.43% respectively by the AM fungus mycorrhizal corn. In addition, the time from planting to harvesting of the sweet corn ranges from 8 weeks to 9 weeks, the protein nutrition of the sweet corn is relatively best in the harvesting period, and the grain yield is relatively highest.

The invention relates to the technical field of forest seedling raising machinery, and discloses a seedling raising and sowing device capable of realizing synchronous application of a forest mycorrhizal agent along with seeds, and by driving two groups of discharging mechanisms of independent storage assemblies for respectively containing forest seeds and mycorrhizal agents to synchronously rotate, the discharging quantity of the two materials and the advancing speed of the device are completely rigidly bound; on the other hand, it is guaranteed that the discharging proportion is constant and accurately matched with the advancing distance from the mechanical structure, finally, the materials are fully mixed through spiral mixing blades in a discharging pipe and then synchronously fall out, and the mycorrhizal agent and seeds are synchronously applied along with the seeds in the same hole; and intermittent extrusion is formed on an extrusion plate on the sliding ring, the sliding ring is driven to drive a material baffle in the storage barrel to synchronously perform circumferential reciprocating turning along with the walking speed in cooperation with rebounding reset of a reset elastic piece in the arc-shaped pipe, and the materials are continuously homogenized to avoid blocking of a discharging channel due to caking and adhesion.

The present application relates to the technical field of fruit tree propagation, in particular to a pear, plum or apricot germplasm resource nursery strip soil cutting method in cold and arid regions, first, one-year-old branches are selected, after disinfection, soaked in rooting mud containing humic acid and growth regulator, then, a cubic soil nub is dug in the rhizosphere of the mother plant, wrapped with non-woven fabric, injected with water-retaining agent, sprayed with humic acid potassium and chitin mixed solution, and inoculated with mycorrhizal fungi, finally, a planting hole is dug according to the set plant spacing, a straw root protection cover is set, the strip with soil is planted, water is poured thoroughly and slow-release fertilizer is buried. The technology effectively protects the root system and promotes rooting through comprehensive treatment, ensuring the rapid and high-quality construction of the resource nursery under the harsh conditions in the cold and arid regions. Through the triple root protection soil cutting technology, the survival rate of pear, plum, apricot and other deciduous fruit tree germplasm resource strips in cold and arid regions is increased from less than 60% of the traditional method to more than 85%.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.