97 results about "Mycorrhiza" patented technology

The invention discloses a mucilaginosa N15 and a method for promoting the germination of dendrobium huoshanense seeds. The mucilaginosa N15 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on October 27, 2025, and the preservation number of the strain is CGMCC No: 42290. The invention further discloses a method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15 and the method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15. The strain can be prepared into a high-activity microbial agent after activation and amplification culture, and the high-activity microbial agent is mixed with dendrobium huoshanense seeds and then sown in a greenhouse, so that the seed germination efficiency can be remarkably improved. By applying the method, the germination rate of the dendrobium huoshanense seeds can be increased to 60% or above, the mycorrhiza infection rate reaches 80% or above, the comprehensive cost is reduced by 30%, the seedling transplanting survival rate reaches 90% or above, and the seedling raising period is shortened to 12-15 months. The method is easy and convenient to operate, low in cost, high in transplanting survival rate and suitable for large-scale seedling raising and resource protection of dendrobium huoshanense, and has important economic and ecological values.

The invention discloses a method for controlling the growth condition of strawberry seedlings, and belongs to the technical field of strawberry seedling raising. The method for controlling the growth condition of the strawberry seedlings comprises the steps that illumination uniformity is adjusted, nutrient soil is prepared for strawberry seedling raising, and illumination uniformity adjustment comprises the steps that different numbers of LED lamp tubes are used for irradiating the growing strawberry seedlings, the illumination uniformity of strawberry seedling canopies is improved, and the energy efficiency ratio is increased; the preparation of the nutrient soil comprises the following steps: mixing and stirring the components of the nutrient soil to ensure the uniform distribution of the raw materials, then putting the nutrient soil into a planting pot, watering and wetting for strawberry seedling planting. The nutrient soil comprises the following components in parts by weight: 35-35 parts of leaf mold; 45 parts of the raw materials and 17 parts of vermiculite powder; 23 parts of perlite, and 23 parts of perlite; 30 parts of carbon nitride composite humic acid; 8 parts of the fertilizer and 5 parts of a mixed fertilizer; 7 parts, 1 part of mycorrhizal inoculant; and 3 parts. According to the method, the growth condition of the strawberry seedlings is cooperatively controlled by adjusting the illumination intensity and preparing the nutrient soil, so that the strawberry seedlings are developed in root system, thick and strong in leaf stems and free of plant diseases and insect pests, and the plant height and the leaf area of the strawberry seedlings are obviously increased after the seedling age is 30 days.

The invention provides a wheat nitrogen fertilizer application reducing and effect increasing method, and belongs to the technical field of biological fertilizers. According to the method, under the background of straw returning to the field, arbuscular mycorrhizal fungi and mushroom residue and excrement decomposed fertilizer are combined as a main body, then peat soil and bentonite are added for wheat cultivation, and application reduction and efficiency improvement of wheat nitrogen fertilizer are achieved; wherein the arbuscular mycorrhizal fungi and the mushroom residue and excrement decomposed fertilizer are combined to promote soil straw degradation and assist nitrogen transfer, the peat soil and the bentonite can improve the water and fertilizer retention capacity of the soil and promote hypha growth and reproduction, available nitrogen in the soil is increased, soil nutrient loss is reduced, and the nutrient absorption capacity is enhanced; further, nitrogen fertilizer absorption and utilization of wheat are improved, and dependence on chemical fertilizer is reduced.

The invention relates to the technical field of forest seedling raising machinery, and discloses a seedling raising and sowing device capable of realizing synchronous application of a forest mycorrhizal agent along with seeds, and by driving two groups of discharging mechanisms of independent storage assemblies for respectively containing forest seeds and mycorrhizal agents to synchronously rotate, the discharging quantity of the two materials and the advancing speed of the device are completely rigidly bound; on the other hand, it is guaranteed that the discharging proportion is constant and accurately matched with the advancing distance from the mechanical structure, finally, the materials are fully mixed through spiral mixing blades in a discharging pipe and then synchronously fall out, and the mycorrhizal agent and seeds are synchronously applied along with the seeds in the same hole; and intermittent extrusion is formed on an extrusion plate on the sliding ring, the sliding ring is driven to drive a material baffle in the storage barrel to synchronously perform circumferential reciprocating turning along with the walking speed in cooperation with rebounding reset of a reset elastic piece in the arc-shaped pipe, and the materials are continuously homogenized to avoid blocking of a discharging channel due to caking and adhesion.

The present application relates to the technical field of fruit tree propagation, in particular to a pear, plum or apricot germplasm resource nursery strip soil cutting method in cold and arid regions, first, one-year-old branches are selected, after disinfection, soaked in rooting mud containing humic acid and growth regulator, then, a cubic soil nub is dug in the rhizosphere of the mother plant, wrapped with non-woven fabric, injected with water-retaining agent, sprayed with humic acid potassium and chitin mixed solution, and inoculated with mycorrhizal fungi, finally, a planting hole is dug according to the set plant spacing, a straw root protection cover is set, the strip with soil is planted, water is poured thoroughly and slow-release fertilizer is buried. The technology effectively protects the root system and promotes rooting through comprehensive treatment, ensuring the rapid and high-quality construction of the resource nursery under the harsh conditions in the cold and arid regions. Through the triple root protection soil cutting technology, the survival rate of pear, plum, apricot and other deciduous fruit tree germplasm resource strips in cold and arid regions is increased from less than 60% of the traditional method to more than 85%.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.

The utility model discloses a device convenient to separate soil mycorrhizal spores, and relates to the technical field of fungus cultivation devices, the device comprises a base and an adjusting assembly, the adjusting assembly is arranged at the top of the base, the adjusting assembly comprises an adjusting frame, and the adjusting frame is fixedly connected to the top of the base. According to the device convenient for separating the soil mycorrhizal spores, a worker can quickly and accurately adjust the vibration frequency according to the characteristics of a soil sample by installing the adjusting assembly, so that the operation convenience is remarkably improved, and the separation requirements of different soil samples can be better met by adjusting the vibration frequency; separation is ensured under the optimal vibration condition, the separation efficiency and spore integrity are improved, damage to spores in the separation process can be reduced easily through accurate vibration frequency adjustment, it can be ensured that the spores are subjected to sufficient vibration to be separated, meanwhile, too large mechanical stress is avoided, the separation quality is improved, and the spore separation efficiency is improved. Therefore, the separation efficiency is prevented from being influenced.

The invention provides bacillus altitudinis CL6 with a morchella esculenta continuous cropping resisting effect and application of the bacillus altitudinis CL6, and belongs to the technical field of microorganisms. The bacillus altitudinis CL6 with a continuous cropping resisting effect on morchella is separated from morchella rhizosphere soil and is preserved in the China Center for Type Culture Collection on March 18, 2025, and the preservation number is CCTCC NO: M 2025516. The bacillus altitudinis CL6 disclosed by the invention can be used as an auxiliary added beneficial microbial agent in the cultivation production of the morchella to resist the continuous cropping harm of the morchella, and finally, the cultivation stability of the morchella is improved. The strain provided by the invention can promote the continuous cropping resistance effect of morchella esculenta and increase the yield of morchella esculenta. And a green and efficient anti-continuous cropping biological solution is provided for the morchella esculenta cultivation industry.

The invention provides boletus nigrum G8 as well as a culture method and application thereof, and belongs to the technical field of edible mushrooms. The preservation number of the boletus nigrum G8 is CGMCC (China General Microbiological Culture Collection Center) No.20233. The invention also provides a corresponding culture method of the boletus nigrum G8 and application of the boletus nigrum G8 in culture of boletus nigrum mycorrhizal seedlings and potting. Compared with other strains, the boletus brownii G8 has the advantages that the growth speed is higher, and the obvious competitive advantage and application value are achieved; sporocarp can be formed in a short time through potting, and continuous mushroom production is achieved.

The invention discloses pseudomonas chlororaphis subsp. Aurantii, a complex microbial inoculant and application of the complex microbial inoculant in relieving continuous cropping obstacles of apples, and belongs to the technical field of agricultural microorganisms. According to the application disclosed by the invention, a strain of pseudomonas chlororaphis subsp. Aurantiaca S74 is separated from mycorrhiza rhizosphere, and the strain has an antagonistic effect on various pathogenic bacteria causing continuous cropping obstacles of apples; the strain has the capabilities of producing protease, secreting siderophores, dissolving inorganic phosphorus, fixing nitrogen and producing ammonia gas. When the strain S74 is applied to apple continuous cropping soil in cooperation with AMF, the number of bacteria in the soil can be increased, the number of pathogenic bacteria can be reduced, plant growth can be promoted, the chlorophyll content of overground leaves of plants can be increased, meanwhile, colonization of AMF in the continuous cropping soil and plant root systems can be promoted, and the strain S74 can relieve apple continuous cropping obstacles in cooperation with AMF. The application method of the strain S74 belongs to the field of biological prevention and control, and provides technical support for comprehensive green prevention and control of apple continuous cropping obstacles.

The application discloses a fungus for promoting growth and flavonoid content accumulation of dendrobium officinale seedlings, wherein the strain is Serendipita sp. WX7, the preservation number is CGMCC NO. 40596, the preservation center is the General Microbiological Center of China, the preservation date is May 29, 2023, the preservation address is No. 3, Xibinxi Road, Chaoyang District, Beijing, and the classification name is Serendipita sp. The fungus has a significant promoting effect on the growth and development of dendrobium officinale seedlings and secondary metabolites, and provides an excellent strain resource for solving the bottleneck problem of low content of effective components of dendrobium officinale in artificial cultivation by using mycorrhizal symbiotic technology.

The present application relates to the field of intelligent agricultural mycorrhizal fungus fertilizer production and application, and discloses a method and application for intelligent soil testing, mycorrhizal fungus fertilizer preparation, quality improvement and soil improvement, which is based on the use of full spectrum direct reading plasma emission spectrometry IPC-OES / ICP-MS, elemental analyzer VarioEL, ion chromatography HPIC combination, and qualitative and quantitative exchange capacity CEC, discloses a method and application for intelligent soil testing, mycorrhizal fungus (AMF) fertilizer preparation, quality improvement and soil improvement by using a chlorophyll nitrogen dynamic monitoring (SPAD value) instrument, a phosphorus online detector and a potassium online detector, which uses a double cultivation and propagation method of mycorrhizal fungus hypha secretion of sphaerocystin, activates plant related gene pathways, improves the quality of crops such as food, vegetables, tobacco leaves and Chinese herbal medicines, and the drought resistance, cold resistance and disease resistance of the crops, forms soil aggregates, increases soil porosity, effectively relieves compaction, and reduces the use amount of chemical fertilizer by 60-80% and pesticide by 70-90%.

The invention belongs to the technical field of orchid seedling culture, and discloses orchid seed matrix particles for field direct seeding and a preparation method, and the matrix particles comprise coco coir powder, peat soil, a porous inorganic matrix, a degradable adhesive, a bio-organic fertilizer, symbiotic bacteria, orchid seeds and water. The matrix particles adopt a core-shell structure, the core contains seeds, symbiotic bacteria and the like for water retention and moisture retention, and the shell is a porous inorganic matrix and the like for guaranteeing ventilation and mechanical strength. The granules can be directly sown in a field, do not need laboratory seedling culture, can realize high-efficiency seed germination and in-situ mycorrhiza colonization, has the seedling rate close to that of traditional sterile seedling transplantation, reduces labor and facility investment, reduces the disease risk, and is suitable for large-scale planting of orchid plants such as bletilla striata and dendrobe.

The application discloses application of an ERF15 gene in regulating and controlling plant arbuscular mycorrhizal symbiosis and belongs to the technical field of biotechnology.The nucleotide sequence of the protein coding region of the ERF15 gene is shown in SEQ ID NO.1 or has at least 70% homology with the sequence shown in SEQ ID NO.1 and the coded protein is functionally equivalent.The application discloses, for the first time, positive regulation of the ERF15 gene in the plant arbuscular mycorrhizal symbiosis, analyzes the molecular mechanism of the ERF15 in promoting phosphorus absorption through the mycorrhizal symbiosis pathway, and provides gene resources and a new idea for plant molecular stress resistance genetic breeding and the like.The application has very important practical value for improving the absorption and utilization efficiency of plants on phosphorus elements, reducing the use of phosphorus fertilizer, and improving the utilization of infertile soil and the like.

The invention discloses a targeted biological bacterium coupling method for comprehensive treatment of coastal vegetable salt damage, heavy metal and pesticide residues and application, and relates to the field of agricultural biotechnology and soil remediation. According to the method, bacillus subtilis, bacillus mucilaginosus, brevibacillus laterosporus, pseudomonas fluorescens, arbuscular mycorrhizal fungi and host original flora extracted from healthy vegetable rhizosphere soil are subjected to coupled symbiotic culture, and target flora with multiple functions is formed. The functional flora is compounded with a nutrient carrier containing potassium humate and fulvic acid, a seaweed extract and a specific salt-resistant additive to prepare the multifunctional water-soluble fertilizer or fungicide. After the compound fertilizer is applied to a coastal vegetable production base, the synergistic effects of degrading soil pesticide residues, passivating heavy metals, enhancing the salt tolerance of vegetables and improving the soil fertility and microbial diversity can be synchronously realized, the compound obstacle problem of coastal vegetable production is fundamentally solved, and the yield, quality and safety of vegetables are guaranteed.

The application discloses a multi-species synergistic truffle mycorrhiza seedling cultivation method and application, and belongs to the technical field of truffle cultivation.The application first adopts Mongolian oak and scots pine as the symbiotic hosts of summer truffle, adopts a two-species synergistic mode, carries out seedling cultivation and cultivation on mycorrhizal fungi, achieves the synergistic effect, significantly shortens the mycorrhizal formation time of mycorrhizal seedlings, improves the infection rate of mycorrhizal and the survival rate of mycorrhizal seedlings, greatly promotes the yield of summer truffle mycorrhizal, and provides technical support for artificial cultivation of summer truffle.

This invention relates to the field of soil pollution control technology, specifically to a microbial-phytoremediation method for heavy metal contaminated soil in mining waste sites. It includes a supporting base layer, on which a leachate collection layer, a heavy metal passivation and isolation layer, a microbial reinforcement layer, and a plant growth layer are sequentially arranged. This invention organically integrates multi-layered structural units to form a spatially ordered structure of leachate drainage—heavy metal passivation—microbial loading—plant growth. It employs immobilized functional microbial agents and embeds multi-parameter sensing devices to establish a monitoring-feedback-control closed-loop system. Simultaneously, hyperaccumulating plants are planted and inoculated with arbuscular mycorrhizal fungi, achieving a shift from an open-loop passive mode to a closed-loop active collaborative mode, avoiding problems such as delayed remediation response, poor synergy, and insufficient long-term stability.

The invention discloses a phosphoric acid modified wheat straw biochar-based slow-release cluster bacterial fertilizer and a preparation method and application thereof.The method comprises the steps that wheat straw serves as a raw material, and phosphoric acid modified biochar (PWBC) with rich oxygen-containing functional groups and a multi-stage pore structure is prepared through phosphoric acid activation and programmed pyrolysis; then taking a granular nitrogen fertilizer as a core, constructing a PWBC coating layer by utilizing a fluidized bed spraying process, inoculating arbuscular mycorrhizal fungi (AMF) spores through low-pressure spraying, and finally, forming PWBC-AMF through secondary encapsulation of sodium alginate. According to the invention, a physical shelter is provided for AMF by using a pore structure of PWBC, and organic fusion of long-acting accurate release of nutrients (the slow release period is greater than or equal to 60 days) and high-power keep-alive of strains is realized. When the method is applied to a pakchoi-radish intercropping planting mode, rhizosphere micro-ecology can be remarkably optimized, interspecific nutrient competition can be relieved, crop biomass can be improved, farmland nitrous oxide emission can be cooperatively reduced, and a scientific scheme is provided for reduction and synergism of chemical fertilizer.

The application discloses a kind of multi-species cooperation's desert vegetation reconstruction method and system, belong to ecological restoration field, based on temperature and humidity change, fine particle soil proportion and wind erosion response lagging behind identification potential wind erosion-thermal response stress coupling area;Microclimate data is collected to construct water vapor dispersion tensor matrix;According to tensor matrix configuration pioneer plant and symbiotic plant combination, and inoculate dormant state mycorrhizal spore, form basic repair unit;Humidity control induction layer is constructed in its periphery, and bacteria proliferation simulation is carried out;Secondary wind erosion blocking structure is constructed by introducing structure induction type plant;Further introduce associated behavior insect and induce plant and fungi-root mutual feeling adjustment device, construct ecological interaction system;Finally output self-maintenance ability analysis index, guide multi-unit parallel repair;The application realizes microclimate regulation, rhizosphere symbiosis, wind erosion protection and the cooperative reconstruction of ecological stability, is suitable for wind erosion strong, drought heavy, microhabitat broken extreme degradation area.

The present application relates to turf, lawn or similar cultivation technical field, disclose a kind of for arid and infertile steep slope protection and ecological restoration vegetation blanket and its use method, in the present application, the inventor finds, with mycorrhiza and root support recovery plant does not become wind roll grass in cryptobiotic state, by the adult of this kind of recovery plant is made into living recovery plant layer with plant fiber and water retaining agent, and is shaded before transplanting, so that its root system no matter how or whether there is root system can be normal planting, so that the plant adult of this kind is batch transplanted on arid and infertile steep slope, and the growth of recovery plant is maintained using moisture-retaining light-shielding layer, nutrient delayed release layer, form a layer with certain water and fertilizer conservation effect " living soil " and carry out preliminary protection to slope surface;After the water and fertilizer condition on slope surface is improved to meet the growth demand of conventional plant, the coating on the surface of seed in seed delay germination layer is degraded, so that seed grows at appropriate time to realize soil and water conservation.

This disclosure relates to biofertilizers. In particular, this disclosure relates to combinations and compositions of biofertilizers containing endophytic bacteria and mycorrhizae, as well as methods of using them.

This invention belongs to the field of biotechnology. To improve the effectiveness of biological control of plant diseases, this invention discloses *Bacillus velezensis* ZF516 and its application in controlling various soil-borne diseases. The *Bacillus velezensis* strain number is ZF516, and its registration number at the China General Microbiological Culture Collection Center is CGMCC No. 30136. This *Bacillus velezensis* strain can prevent and / or treat tomato bacterial canker, tomato bacterial wilt, or cowpea root rot, or can be used to prepare products for the prevention and / or treatment of these diseases. This strain also exhibits good inhibitory effects against *Fusarium oxysporum*, *Diplostomum spp.*, *Rhizoctonia solani*, *Fusarium solanum*, *Corynebacterium multiflorum*, *Botrytis cinerea*, *Agrobacterium tumefaciens*, *Pectinobacter brasiliensis*, *Ralstonia solanacearum*, *Streptomyces spp.*, etc.

The invention relates to the technical field of agricultural biotechnology and culture substrates, in particular to a compound microorganism culture substrate special for blueberries and a preparation method and application of the compound microorganism culture substrate. The invention provides a special compound microorganism culture medium for blueberries. The special compound microorganism culture medium comprises the following components: a basic culture medium and a compound bacterium agent, the invention relates to a compound microbial inoculant, which is characterized in that the compound microbial inoculant is an azalea mycorrhizal fungi (Eryzophyllum commune), paenibacillus mucilaginosus (Paenibacillus mucilaginosus) and Trichoderma harzianum (Trichoderma harzianum). By organically combining excellent physicochemical properties of the substrate with specific beneficial microorganism functions, the synergistic effect of '1 + 1 + 1gt, 3' is realized, a set of complete solution is provided for green, high-quality and efficient cultivation of blueberries, and the method has important popularization and application values and wide market prospects.

The application discloses an indoor seedling raising method of Tricholoma mongolicum, and relates to the technical field of indoor seedling raising, which comprises the following steps: preparing a low-nutrient symbiotic germination culture medium, taking water and agar as the basis, containing 0-5 g / L sugar substances and humus soil extract, and forming a sterile environment beneficial to the symbiosis of fungi; selecting associated fungi to prepare mycelium fragment suspension as symbiotic inoculants through liquid fermentation; performing surface disinfection and sterile ultrasonic treatment on Tricholoma mongolicum seeds; sowing the seeds on the surface of the culture medium and inoculating the symbiotic inoculants under sterile conditions, and culturing under artificial climate control of temperature and light, so as to promote the symbiotic relationship between the fungi and the seeds; and obtaining symbiotic seedlings after the seeds swell and differentiate into protocorms or young leaves. The symbiotic germination mechanism of associated fungi is introduced into the indoor sterile culture system, the emergence rate can reach more than 90%, the emergence uniformity is high, the seedlings are not limited by seasons and climate, the seedlings are self-brought mycorrhizal roots, the transplanting survival rate is high, and the reliable seedling guarantee is provided for the large-scale artificial planting of Tricholoma mongolicum.

The invention provides a biological stimulant for regulating and controlling micro-ecological activity of acid soil rhizosphere as well as a preparation method and application of the biological stimulant, and particularly provides the biological stimulant which comprises high fructose corn syrup, tea polyphenol and 5-aminolevulinic acid. The biostimulant further comprises an amino acid and an oligosaccharide. The biological stimulant provided by the invention can improve the pH of rhizosphere soil, reduce exchangeable aluminum ions, improve soil respiration and mycorrhizal spore density, and realize yield increase and efficiency increase.

The invention relates to the technical field of camellia oleifera cultivation, and discloses a cultivation method for effectively improving the survival rate of camellia oleifera seedlings, which is characterized by comprising the following steps: mixing and decomposing 40-50% of humus soil, 20-25% of decomposed camellia oleifera shell matrix, 10-15% of perlite, 8-12% of vermiculite, 3-5% of mycorrhizal inoculant and 2-3% of slow release fertilizer in percentage by volume, and regulating the pH value to 5.0-5.5 to prepare a special seedling culture matrix; the method comprises the following steps: soaking camellia oleifera seeds in warm water, disinfecting, dibbling in a seedling raising pot, covering a substrate, watering, controlling the temperature, humidity and illumination of a seedling raising environment, spraying a foliar fertilizer after seedling emergence, watering a rooting nutrient solution, carrying out stress resistance induction and seedling hardening, carrying out seedling hardening before transplanting, watering seedling water, transplanting to a pretreated land parcel with soil, building a shading shed after transplanting, and controlling the temperature and humidity in a rejuvenation period; and after seedling recovery, removing the shed and fertilizing. According to the method, the combination of the special seedling culture substrate and precise nutrition supply is adopted, so that the root development of the camellia oleifera seedlings is remarkably enhanced.

The application discloses a mycorrhizal edible mushroom under-forest cultivation fertilizing device and relates to the technical field of edible mushroom planting. The device comprises a moving assembly, water and fertilizer spraying assemblies are arranged on the two sides of the moving assembly, water-soluble fertilizer adding assemblies, push-pull driving assemblies and water and fertilizer containing mechanisms are sequentially arranged on the top of the moving assembly from back to front, two groups of symmetrically distributed adjustable mixing mechanisms are arranged in the water and fertilizer containing mechanisms, the push-pull driving assembly comprises a fixed frame fixedly arranged on the top center of the bearing vehicle, and a reciprocating screw is rotatably nested on the inner top of the fixed frame through a bearing. The double horizontal containing barrels can be used for synchronously applying and preparing liquid fertilizer, the stop feeding operation during the fertilizing process is not needed, the use difficulty is reduced, the continuous fertilizing operation of the mycorrhizal edible mushroom can be realized, and the fertilizing efficiency is greatly improved.

This invention belongs to the field of *Coptis chinensis* cultivation technology, specifically relating to a cultivation method for increasing the berberine content in *Coptis chinensis*, comprising the following steps: seedling cultivation, transplanting, microbial inoculation, light management, fertilization management, staged drought stress induction, water management, and harvesting; the microbial inoculation involves simultaneously applying a compound microbial agent containing *Bacillus amyloliquefaciens*, arbuscular mycorrhizal fungi, and plant rhizosphere growth-promoting bacteria at the time of transplanting; the light management involves shading combined with LED supplemental lighting; the staged drought stress induction involves controlling the relative soil moisture content to 45%-55% for 7-10 days after transplanting, 60-75 days after transplanting. This invention, through the synergistic effect of microbial inoculation, light quality regulation, and drought stress induction, can significantly increase the berberine content in the roots of *Coptis chinensis* by more than 120% compared to conventional cultivation methods, and is standardized, green, and sustainable, suitable for large-scale cultivation and promotion.

This invention discloses a slope ecological restoration substrate based on multi-level synergistic solidification and a layered spraying method, belonging to the field of geotechnical engineering and ecological restoration technology. This invention proposes a three-layer synergistic substrate system and dynamic construction process. In the substrate, the bottom layer uses modified volcanic ash and nano-silica sol as cementing materials to enhance rock surface penetration and adhesion; the middle layer uses chitosan slow-release particles and a gradient moisture content design to achieve phased nutrient release and interlayer stress buffering; the surface layer combines a 3D honeycomb biochar carrier and a biodegradable PLA grid, simultaneously loading grass seeds and mycorrhizal fungi to form a "physical-biological" composite anchoring structure. The construction method of this invention includes high-frequency micro-vibration infiltration, layered gradient spraying, and photosensitive non-woven fabric covering technology to ensure the substrate's resistance to rain erosion and rapid vegetation cover. This invention combines mechanical stability and ecological compatibility, is suitable for the restoration of steep rock slopes, and significantly reduces maintenance costs and environmental pollution risks.

The invention relates to mycorrhizal fungi and application thereof in promoting paphiopedilum seed germination and rapid seedling, and belongs to the technical field of microorganisms. The mycorrhizal fungus is MLXY010JD, the taxonomic name of the mycorrhizal fungus is Tulasnella sp., the strain is preserved in Wuhan University, Wuhan, China on September 15, 2025, and the preservation number of the strain is CCTCC NO: M 20252049. The invention also provides a method for promoting seed germination and rapid seedling formation of paphiopedilum by using the strain. The method comprises the following steps: performing symbiotic culture on seeds and mycorrhizal fungi to induce germination to form seedlings; carrying out symbiotic culture on the MLXY010JD and the seedlings to promote the growth of the seedlings; transferring to a specific culture medium for continuous culture; finally, the whole seedling raising process is completed through seedling hardening and transplanting management. The method is suitable for various paphiopedilum species and provides support for artificial breeding and field regression of paphiopedilum.