134 results about "Mycorrhiza" patented technology

The invention relates to the technical field of agricultural biology, and discloses a microbial agent for preventing and treating fructus aurantii scab and a preparation method of the microbial agent. The microbial agent comprises the following raw materials in percentage by weight: 10-13% of paenibacillus mucilaginosus; 15%-20% of trichoderma sp. 7%-10% of paecilomyces lilacinus; 5%-8% of bacillus pumilus; 7%-9% of mycorrhizal fungi; 5%-8% of streptomyces; 5%-9% of a coptis chinensis extract; 4%-7% of a sophora flavescens extract; 2%-4% of realgar; 1%-3% of a rheum officinale extract; 10%-13% of soybean meal; 5%-8% of a water-soluble fertilizer; 7%-9% of potassium fulvate; 1%-3% of chitosan; 0.5%-1% of trace elements; 3%-8% of humic acid; the raw materials are subjected to microbial flora amplification culture, plant antibacterial component light-shielding mixing and low-temperature mixing to effectively protect the microbial activity of the microbial agent, and step-by-step operation can avoid damage of high temperature and mechanical shearing to microorganisms, so that the microbial activity of the microbial agent is effectively protected. And the loss of microbial active ingredients is reduced by vacuum drying and low-temperature granulation processes.

The invention discloses a growth-promoting rhizobacteria, a growth-promoting rhizobacteria microbial inoculum and application thereof, and belongs to the technical field of microorganisms, the growth-promoting rhizobacteria is Pseudomonas mohnii, the strain number is YQ142, the classification name is Pseudomonas mohnii, and the growth-promoting rhizobacteria is preserved in China General Microbiological Culture Collection Center on April 7, 2025, and the preservation number is CGMCC No.34110. The invention further discloses a preparation method of the growth-promoting rhizobacteria. The growth-promoting rhizobacteria has strong nitrogen fixation and phosphate solubilizing capabilities, can promote the growth of ryegrass, oat and corn, and improves the saline-alkali stress resistance and drought stress resistance of ryegrass.

The invention relates to the field of soil remediation conditioners, and particularly provides a soil remediation conditioner for durian breeding as well as preparation and application of the soil remediation conditioner. The soil remediation conditioner is prepared from the following components in parts by weight: 35 to 45 parts of organic matter fertilizer, 18 to 22 parts of biochar, 22 to 28 parts of humic acid, 8 to 12 parts of vermiculite, 10 to 14 parts of perlite, 12 to 18 parts of shell powder, 6 to 10 parts of mycorrhizal fungi, EM bacteria and other mixed bacteria, 8 to 12 parts of wormcast, 4 to 6 parts of amino acid chelate solution, 8 to 12 parts of coco coir and 4 to 6 parts of adhesive. The specific surface area of the biochar is 32 m < 2 > / g, and the particle size is 40 meshes; the soil remediation conditioner is applied to durian planting soil remediation, has the effects of improving the physical structure of soil, improving the air permeability and water retention of the soil, increasing the fertility of the soil and providing rich nutrition for breeding and growth of durian seedlings, and has great application potential for the durian industry.

The invention discloses an ancient tree health state monitoring method based on digital twinning, and relates to the field of ancient tree health monitoring, and the method comprises the steps: collecting point cloud data and texture data of an ancient tree, and constructing an initial three-dimensional model of the ancient tree; a micro ion selective electrode array is deployed in an ancient tree root distribution area, Ca2 and K + ion flow velocity changes are detected through a constant potential rectifier, and the mycorrhizal symbiosis index is calculated in combination with soil temperature and humidity data; uploading the mycorrhizal symbiosis index to a digital twinborn body, and displaying the active state of a root system area in an initial three-dimensional model of the ancient tree in a three-dimensional thermodynamic diagram form; a 16-channel MOX gas sensor electronic nose array is installed on the surface of a trunk of an ancient tree, and volatile organic compound (VOC) concentration data of the ancient tree are collected. According to the invention, a micro electrode array with a 45-degree inclination angle is adopted to non-destructively monitor the ion flow velocity of a root system through the mycorrhizal symbiosis index detection unit, and a three-dimensional thermodynamic diagram is generated in combination with an inverse distance weighted interpolation algorithm, so that dynamic visualization of underground mycorrhizal activity is realized.

The invention discloses a mucilaginosa N15 and a method for promoting the germination of dendrobium huoshanense seeds. The mucilaginosa N15 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on October 27, 2025, and the preservation number of the strain is CGMCC No: 42290. The invention further discloses a method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15 and the method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15. The strain can be prepared into a high-activity microbial agent after activation and amplification culture, and the high-activity microbial agent is mixed with dendrobium huoshanense seeds and then sown in a greenhouse, so that the seed germination efficiency can be remarkably improved. By applying the method, the germination rate of the dendrobium huoshanense seeds can be increased to 60% or above, the mycorrhiza infection rate reaches 80% or above, the comprehensive cost is reduced by 30%, the seedling transplanting survival rate reaches 90% or above, and the seedling raising period is shortened to 12-15 months. The method is easy and convenient to operate, low in cost, high in transplanting survival rate and suitable for large-scale seedling raising and resource protection of dendrobium huoshanense, and has important economic and ecological values.

The invention relates to the technical field of cultivation of turfs, lawns or analogues, and discloses a vegetation blanket for slope protection and ecological restoration of drought and barren steep slopes and a use method of the vegetation blanket. Adults of the resuscitated plants, plant fibers and a water-retaining agent are prepared into a living resuscitated plant layer together, and the living resuscitated plant layer is subjected to yin cultivation before transplanting, so that normal planting can be carried out regardless of the orientation or existence of root systems, the plant adults are transplanted on a drought and barren abrupt slope in batches, and the survival rate of the plants is increased. A moisturizing and shading layer and a nutrient delayed release layer are adopted to maintain growth of the resuscitated plants, a layer of living soil with a certain water and fertilizer retention effect is formed, and the slope surface is preliminarily protected; after the water and fertilizer conditions on the slope surface are improved to meet the conventional plant growth requirements, coatings on the surfaces of the seeds in the seed delayed germination layer are degraded, so that the seeds germinate and grow at a proper time to realize water and soil conservation.

The invention discloses a method for controlling the growth condition of strawberry seedlings, and belongs to the technical field of strawberry seedling raising. The method for controlling the growth condition of the strawberry seedlings comprises the steps that illumination uniformity is adjusted, nutrient soil is prepared for strawberry seedling raising, and illumination uniformity adjustment comprises the steps that different numbers of LED lamp tubes are used for irradiating the growing strawberry seedlings, the illumination uniformity of strawberry seedling canopies is improved, and the energy efficiency ratio is increased; the preparation of the nutrient soil comprises the following steps: mixing and stirring the components of the nutrient soil to ensure the uniform distribution of the raw materials, then putting the nutrient soil into a planting pot, watering and wetting for strawberry seedling planting. The nutrient soil comprises the following components in parts by weight: 35-35 parts of leaf mold; 45 parts of the raw materials and 17 parts of vermiculite powder; 23 parts of perlite, and 23 parts of perlite; 30 parts of carbon nitride composite humic acid; 8 parts of the fertilizer and 5 parts of a mixed fertilizer; 7 parts, 1 part of mycorrhizal inoculant; and 3 parts. According to the method, the growth condition of the strawberry seedlings is cooperatively controlled by adjusting the illumination intensity and preparing the nutrient soil, so that the strawberry seedlings are developed in root system, thick and strong in leaf stems and free of plant diseases and insect pests, and the plant height and the leaf area of the strawberry seedlings are obviously increased after the seedling age is 30 days.

The invention provides a wheat nitrogen fertilizer application reducing and effect increasing method, and belongs to the technical field of biological fertilizers. According to the method, under the background of straw returning to the field, arbuscular mycorrhizal fungi and mushroom residue and excrement decomposed fertilizer are combined as a main body, then peat soil and bentonite are added for wheat cultivation, and application reduction and efficiency improvement of wheat nitrogen fertilizer are achieved; wherein the arbuscular mycorrhizal fungi and the mushroom residue and excrement decomposed fertilizer are combined to promote soil straw degradation and assist nitrogen transfer, the peat soil and the bentonite can improve the water and fertilizer retention capacity of the soil and promote hypha growth and reproduction, available nitrogen in the soil is increased, soil nutrient loss is reduced, and the nutrient absorption capacity is enhanced; further, nitrogen fertilizer absorption and utilization of wheat are improved, and dependence on chemical fertilizer is reduced.

The invention relates to the technical field of forest seedling raising machinery, and discloses a seedling raising and sowing device capable of realizing synchronous application of a forest mycorrhizal agent along with seeds, and by driving two groups of discharging mechanisms of independent storage assemblies for respectively containing forest seeds and mycorrhizal agents to synchronously rotate, the discharging quantity of the two materials and the advancing speed of the device are completely rigidly bound; on the other hand, it is guaranteed that the discharging proportion is constant and accurately matched with the advancing distance from the mechanical structure, finally, the materials are fully mixed through spiral mixing blades in a discharging pipe and then synchronously fall out, and the mycorrhizal agent and seeds are synchronously applied along with the seeds in the same hole; and intermittent extrusion is formed on an extrusion plate on the sliding ring, the sliding ring is driven to drive a material baffle in the storage barrel to synchronously perform circumferential reciprocating turning along with the walking speed in cooperation with rebounding reset of a reset elastic piece in the arc-shaped pipe, and the materials are continuously homogenized to avoid blocking of a discharging channel due to caking and adhesion.

The present application relates to the technical field of fruit tree propagation, in particular to a pear, plum or apricot germplasm resource nursery strip soil cutting method in cold and arid regions, first, one-year-old branches are selected, after disinfection, soaked in rooting mud containing humic acid and growth regulator, then, a cubic soil nub is dug in the rhizosphere of the mother plant, wrapped with non-woven fabric, injected with water-retaining agent, sprayed with humic acid potassium and chitin mixed solution, and inoculated with mycorrhizal fungi, finally, a planting hole is dug according to the set plant spacing, a straw root protection cover is set, the strip with soil is planted, water is poured thoroughly and slow-release fertilizer is buried. The technology effectively protects the root system and promotes rooting through comprehensive treatment, ensuring the rapid and high-quality construction of the resource nursery under the harsh conditions in the cold and arid regions. Through the triple root protection soil cutting technology, the survival rate of pear, plum, apricot and other deciduous fruit tree germplasm resource strips in cold and arid regions is increased from less than 60% of the traditional method to more than 85%.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.

This invention provides the application of mycorrhizal fungi in regulating carbon allocation in Dendrobium officinale plants, belonging to the field of orchid mycorrhizal symbiosis technology. The invention includes the following steps: preparing a mycorrhizal fungal inoculant, wherein the mycorrhizal fungi are one or both of *Cladosporium halophilum* or *Dendrobium nobile*; soaking the roots of Dendrobium officinale tissue culture seedlings in the mycorrhizal fungal inoculant, and then transplanting them into a culture medium for cultivation. This invention, by inoculating the roots of Dendrobium officinale with mycorrhizal fungi, promotes mycorrhizal symbiosis and growth of Dendrobium officinale, while also inducing the expression of certain specific genes in Dendrobium officinale, activating a series of secondary metabolic pathways, thereby causing changes in the carbon allocation of the aboveground parts and roots of Dendrobium officinale seedlings, further influencing and regulating the biosynthesis of Dendrobium officinale polysaccharides, which is beneficial for further elucidating the molecular regulatory mechanism of mycorrhizal symbiosis on sugar metabolism in Dendrobium officinale.

The invention relates to a tool and a method for separating, sterilizing and filtering arbuscular mycorrhizal fungi monospores. The device comprises a microoperation module and a multi-stage filtering module which work cooperatively. Wherein the microoperation module comprises a microsuction device and a filtering module, the filtering module comprises an upper filtering section and a lower filtering section, the upper filtering section comprises a first cylindrical section, a second cylindrical section and a third cylindrical section which are coaxially arranged and sequentially connected, and a filter screen is arranged at the end of the third cylindrical section; the lower filtering section comprises a fourth filtering section and a fifth filtering section which are coaxially arranged and connected with each other, the fourth filtering section is used for sleeving the third filtering section, and the fourth filtering section and the fifth filtering section jointly form a filter for filtering spores extracted by the micro-suction device. The tool has the innovativeness that the tool can effectively solve the problem of single separation of AMF fungal spores, and loss of the spores is reduced in the sterilization process.

The invention relates to the technical field of fertilization, in particular to an edible mushroom planting automatic fertilizer applicator which comprises a fertilization moving vehicle, a plurality of moving wheels are arranged at the bottom of the fertilization moving vehicle and used for driving the fertilization moving vehicle to move, and a plurality of fertilizer storage cylinders are mounted on the upper end face of the fertilization moving vehicle and used for storing fertilizer in the fertilizer storage cylinders. A plurality of supporting frames are slidably installed on the upper end face of the fertilization moving trolley, the supporting frames are arranged on the sides, away from the fertilizer storage barrel, of the supporting frames, and feeding needle heads are arranged on the sides, away from the fertilizer storage barrel, of the supporting frames and can move up and down on the side of the fertilization moving trolley so as to penetrate into edible fungus soil; the edible mushroom soil is directly fertilized, the feeding needle head can penetrate into the edible mushroom soil, and the fertilizer in the fertilizer storage barrel is introduced into the edible mushroom soil, so that the fertilizer can be in direct contact with mycorrhiza, the combination of the mycorrhiza and fertilizer nutrients is ensured, and the self growth of edible mushrooms is facilitated.

The utility model discloses a device convenient to separate soil mycorrhizal spores, and relates to the technical field of fungus cultivation devices, the device comprises a base and an adjusting assembly, the adjusting assembly is arranged at the top of the base, the adjusting assembly comprises an adjusting frame, and the adjusting frame is fixedly connected to the top of the base. According to the device convenient for separating the soil mycorrhizal spores, a worker can quickly and accurately adjust the vibration frequency according to the characteristics of a soil sample by installing the adjusting assembly, so that the operation convenience is remarkably improved, and the separation requirements of different soil samples can be better met by adjusting the vibration frequency; separation is ensured under the optimal vibration condition, the separation efficiency and spore integrity are improved, damage to spores in the separation process can be reduced easily through accurate vibration frequency adjustment, it can be ensured that the spores are subjected to sufficient vibration to be separated, meanwhile, too large mechanical stress is avoided, the separation quality is improved, and the spore separation efficiency is improved. Therefore, the separation efficiency is prevented from being influenced.

The invention provides bacillus altitudinis CL6 with a morchella esculenta continuous cropping resisting effect and application of the bacillus altitudinis CL6, and belongs to the technical field of microorganisms. The bacillus altitudinis CL6 with a continuous cropping resisting effect on morchella is separated from morchella rhizosphere soil and is preserved in the China Center for Type Culture Collection on March 18, 2025, and the preservation number is CCTCC NO: M 2025516. The bacillus altitudinis CL6 disclosed by the invention can be used as an auxiliary added beneficial microbial agent in the cultivation production of the morchella to resist the continuous cropping harm of the morchella, and finally, the cultivation stability of the morchella is improved. The strain provided by the invention can promote the continuous cropping resistance effect of morchella esculenta and increase the yield of morchella esculenta. And a green and efficient anti-continuous cropping biological solution is provided for the morchella esculenta cultivation industry.

The present invention belongs to the field of composite materials technology, specifically relating to an edible mycorrhizal glycoconjugate-graphene conductive film and its preparation method. This invention uses chitosan-glucan complex (CGC), a root glycoconjugate extracted from the mycorrhizae of Flammulina velutipes, as raw material. Extracting CGC from the mycorrhizae requires only mild acid and alkali treatment to remove components such as proteins, minerals, and lipids, while also minimizing damage to the CGC and improving its purity. The prepared CGC, when used as a dispersant, improves graphene agglomeration. Compared to graphene without CGC, at the optimal concentration of CGC added, the median particle size decreased by 43.11%, and the absolute value of the zeta potential increased by 160.07%. The addition of CGC to graphene significantly enhances its dispersibility, improving the tensile strength and conductivity of the CGC-graphene film.

The invention provides boletus nigrum G8 as well as a culture method and application thereof, and belongs to the technical field of edible mushrooms. The preservation number of the boletus nigrum G8 is CGMCC (China General Microbiological Culture Collection Center) No.20233. The invention also provides a corresponding culture method of the boletus nigrum G8 and application of the boletus nigrum G8 in culture of boletus nigrum mycorrhizal seedlings and potting. Compared with other strains, the boletus brownii G8 has the advantages that the growth speed is higher, and the obvious competitive advantage and application value are achieved; sporocarp can be formed in a short time through potting, and continuous mushroom production is achieved.

The invention discloses pseudomonas chlororaphis subsp. Aurantii, a complex microbial inoculant and application of the complex microbial inoculant in relieving continuous cropping obstacles of apples, and belongs to the technical field of agricultural microorganisms. According to the application disclosed by the invention, a strain of pseudomonas chlororaphis subsp. Aurantiaca S74 is separated from mycorrhiza rhizosphere, and the strain has an antagonistic effect on various pathogenic bacteria causing continuous cropping obstacles of apples; the strain has the capabilities of producing protease, secreting siderophores, dissolving inorganic phosphorus, fixing nitrogen and producing ammonia gas. When the strain S74 is applied to apple continuous cropping soil in cooperation with AMF, the number of bacteria in the soil can be increased, the number of pathogenic bacteria can be reduced, plant growth can be promoted, the chlorophyll content of overground leaves of plants can be increased, meanwhile, colonization of AMF in the continuous cropping soil and plant root systems can be promoted, and the strain S74 can relieve apple continuous cropping obstacles in cooperation with AMF. The application method of the strain S74 belongs to the field of biological prevention and control, and provides technical support for comprehensive green prevention and control of apple continuous cropping obstacles.

The application discloses a fungus for promoting growth and flavonoid content accumulation of dendrobium officinale seedlings, wherein the strain is Serendipita sp. WX7, the preservation number is CGMCC NO. 40596, the preservation center is the General Microbiological Center of China, the preservation date is May 29, 2023, the preservation address is No. 3, Xibinxi Road, Chaoyang District, Beijing, and the classification name is Serendipita sp. The fungus has a significant promoting effect on the growth and development of dendrobium officinale seedlings and secondary metabolites, and provides an excellent strain resource for solving the bottleneck problem of low content of effective components of dendrobium officinale in artificial cultivation by using mycorrhizal symbiotic technology.

The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a tricholoma matsutake culture medium based on tunnel fermentation and a tricholoma matsutake semi-artificial cultivation method. The tricholoma matsutake culture medium comprises 45-55% of wheat straw, 10-20% of soybean meal, 19-21% of Korean pine leaves and 14-16% of leaf mold; a secondary tunnel fermentation process is adopted to prepare the feed additive, high throughput is adopted in primary fermentation, and low throughput is adopted in secondary fermentation. The semi-artificial cultivation method of the tricholoma matsutake comprises the following steps: inoculating a tricholoma matsutake strain into a liquid culture medium, fermenting to obtain a liquid strain, then mixing the liquid strain with the tricholoma matsutake culture medium, and pre-culturing to obtain an inoculation material; burying the inoculation material in a tree pit, covering a mixture of decomposed pine needles and black sandy soil and living moss on the inoculation material, and culturing. The tricholoma matsutake culture medium is high in fermentation efficiency and low in fermentation energy consumption, the mycelium planting rate is high when the tricholoma matsutake culture medium is used for tricholoma matsutake culture, and the tricholoma matsutake semi-artificial cultivation method is high in mycorrhiza forming rate, high in annual yield and low in sporocarp malformation rate.

The present invention relates to the field of wind erosion control technology, and specifically to a method for artificial directional filling and maintenance control applicable to wind-eroded sand beaches, comprising: generating a wind erosion risk distribution heat map based on beach elevation point cloud data and historical wind erosion vector maps, marking strong wind erosion areas as targeted maintenance areas; using a particle size layer filling device to sequentially construct a coarse sand layer, a medium sand layer, and a fine sand layer based on the results of in-situ sand particle size analysis; shaping a continuous wavy wind-guiding ridge on the surface of the filling area, controlling its direction with the direction of the main harmful wind and meeting the ridge height-width ratio and slope requirements; evenly distributing diamond-shaped vegetation troughs on the leeward side of the ridge, filling it with a sand-humus-water-retaining agent mixed matrix, planting salt-alkali-tolerant shrubs, and laying a high-density mycorrhizal fiber mat activated by seaweed extract, ultimately constructing a gridded biological sand fixation system. The present invention achieves high-precision identification of wind-eroded sand beaches, coordinated control of structurally stable filling and ecological restoration, and has the advantages of strong stability and wide applicability.

A genetically modified bacterium for excreting fixed nitrogen (in the form of ammonia) is disclosed. The bacterium can be made by deleting at least a portion of the nifL gene of a diazotrophic γ-proteobacterium, and inserting a promoter sequence into the diazotrophic γ-proteobacterium genome that is placed and oriented to direct transcription of the rnf1 gene complex. The resulting genetically modified bacterium excretes ammonia constitutively and at a greater rate than the wild type bacterium, and can be used to make biofertilizers to stimulate plant growth. The biofertilizers may contain a culture of the bacteria, or a co-culture of the bacteria and a mycorrhizal fungus.

The present application relates to the field of intelligent agricultural mycorrhizal fungus fertilizer production and application, and discloses a method and application for intelligent soil testing, mycorrhizal fungus fertilizer preparation, quality improvement and soil improvement, which is based on the use of full spectrum direct reading plasma emission spectrometry IPC-OES / ICP-MS, elemental analyzer VarioEL, ion chromatography HPIC combination, and qualitative and quantitative exchange capacity CEC, discloses a method and application for intelligent soil testing, mycorrhizal fungus (AMF) fertilizer preparation, quality improvement and soil improvement by using a chlorophyll nitrogen dynamic monitoring (SPAD value) instrument, a phosphorus online detector and a potassium online detector, which uses a double cultivation and propagation method of mycorrhizal fungus hypha secretion of sphaerocystin, activates plant related gene pathways, improves the quality of crops such as food, vegetables, tobacco leaves and Chinese herbal medicines, and the drought resistance, cold resistance and disease resistance of the crops, forms soil aggregates, increases soil porosity, effectively relieves compaction, and reduces the use amount of chemical fertilizer by 60-80% and pesticide by 70-90%.

The invention provides an open type tissue culture method of bamboo reed, which comprises the following tissue culture steps: S1, preparation of a gradient antibacterial culture medium: (1) preparation of the gradient antibacterial culture medium: an MS culture medium, 0.5 mg / L 6-BA and 0.1 mg / L NAA are added, 0.1% of tea polyphenol aqueous extract is added, and 0.03% of water-soluble chitosan oligosaccharide is added. Traditional chemical disinfection is replaced with a natural gradient antibacterial system, the dual functions of pollution prevention and control and root protection are achieved by combining a symbiotic bacterium slow release technology, and the stress resistance and lignification characteristics of plants are synchronously improved through the synergistic effect of salinity gradient stimulation and environmental signal molecules; a light-gas intelligent regulation and control system is developed to simulate the optimal growth condition, and a transplanting survival guarantee system is constructed in cooperation with a mycorrhiza pre-activation technology; the dependence of traditional tissue culture on a sterile environment is broken through, and the seedling culture efficiency, the ecological compatibility and the resource utilization level are comprehensively improved under an open condition.

The invention belongs to the technical field of orchid seedling culture, and discloses orchid seed matrix particles for field direct seeding and a preparation method, and the matrix particles comprise coco coir powder, peat soil, a porous inorganic matrix, a degradable adhesive, a bio-organic fertilizer, symbiotic bacteria, orchid seeds and water. The matrix particles adopt a core-shell structure, the core contains seeds, symbiotic bacteria and the like for water retention and moisture retention, and the shell is a porous inorganic matrix and the like for guaranteeing ventilation and mechanical strength. The granules can be directly sown in a field, do not need laboratory seedling culture, can realize high-efficiency seed germination and in-situ mycorrhiza colonization, has the seedling rate close to that of traditional sterile seedling transplantation, reduces labor and facility investment, reduces the disease risk, and is suitable for large-scale planting of orchid plants such as bletilla striata and dendrobe.

The application discloses application of an ERF15 gene in regulating and controlling plant arbuscular mycorrhizal symbiosis and belongs to the technical field of biotechnology.The nucleotide sequence of the protein coding region of the ERF15 gene is shown in SEQ ID NO.1 or has at least 70% homology with the sequence shown in SEQ ID NO.1 and the coded protein is functionally equivalent.The application discloses, for the first time, positive regulation of the ERF15 gene in the plant arbuscular mycorrhizal symbiosis, analyzes the molecular mechanism of the ERF15 in promoting phosphorus absorption through the mycorrhizal symbiosis pathway, and provides gene resources and a new idea for plant molecular stress resistance genetic breeding and the like.The application has very important practical value for improving the absorption and utilization efficiency of plants on phosphorus elements, reducing the use of phosphorus fertilizer, and improving the utilization of infertile soil and the like.

The invention discloses a targeted biological bacterium coupling method for comprehensive treatment of coastal vegetable salt damage, heavy metal and pesticide residues and application, and relates to the field of agricultural biotechnology and soil remediation. According to the method, bacillus subtilis, bacillus mucilaginosus, brevibacillus laterosporus, pseudomonas fluorescens, arbuscular mycorrhizal fungi and host original flora extracted from healthy vegetable rhizosphere soil are subjected to coupled symbiotic culture, and target flora with multiple functions is formed. The functional flora is compounded with a nutrient carrier containing potassium humate and fulvic acid, a seaweed extract and a specific salt-resistant additive to prepare the multifunctional water-soluble fertilizer or fungicide. After the compound fertilizer is applied to a coastal vegetable production base, the synergistic effects of degrading soil pesticide residues, passivating heavy metals, enhancing the salt tolerance of vegetables and improving the soil fertility and microbial diversity can be synchronously realized, the compound obstacle problem of coastal vegetable production is fundamentally solved, and the yield, quality and safety of vegetables are guaranteed.

The application discloses a multi-species synergistic truffle mycorrhiza seedling cultivation method and application, and belongs to the technical field of truffle cultivation.The application first adopts Mongolian oak and scots pine as the symbiotic hosts of summer truffle, adopts a two-species synergistic mode, carries out seedling cultivation and cultivation on mycorrhizal fungi, achieves the synergistic effect, significantly shortens the mycorrhizal formation time of mycorrhizal seedlings, improves the infection rate of mycorrhizal and the survival rate of mycorrhizal seedlings, greatly promotes the yield of summer truffle mycorrhizal, and provides technical support for artificial cultivation of summer truffle.

This invention relates to the field of soil pollution control technology, specifically to a microbial-phytoremediation method for heavy metal contaminated soil in mining waste sites. It includes a supporting base layer, on which a leachate collection layer, a heavy metal passivation and isolation layer, a microbial reinforcement layer, and a plant growth layer are sequentially arranged. This invention organically integrates multi-layered structural units to form a spatially ordered structure of leachate drainage—heavy metal passivation—microbial loading—plant growth. It employs immobilized functional microbial agents and embeds multi-parameter sensing devices to establish a monitoring-feedback-control closed-loop system. Simultaneously, hyperaccumulating plants are planted and inoculated with arbuscular mycorrhizal fungi, achieving a shift from an open-loop passive mode to a closed-loop active collaborative mode, avoiding problems such as delayed remediation response, poor synergy, and insufficient long-term stability.