173 results about "Mycorrhiza" patented technology

The invention discloses a method for restoring soil heavy metal pollution by coupling soybean urease with a charcoal cementing agent, and relates to the technical field of soil heavy metal pollution restoration, and the method comprises the following steps: dynamic microbiome regulation and control: introducing photosensitive protein into soil functional bacteria; the acyl homoserine lactone analogue is synthesized and applied to soil, and is competitively combined with a microbial quorum sensing receptor to block an inhibitor to secrete a signal channel; according to the method, microbial urease secretion is regulated and controlled through optogenetics, an inhibitor signal is blocked through quorum sensing interference, and a microbiome is precisely regulated and controlled; the pH-responsive nanocapsule releases an inhibitor in a targeted manner, and the magnetic-responsive nanochain dynamically captures interfering bacteria, so that the limitation of passive adsorption is broken through; the gene editing plant releases coumarin to inhibit fungal metabolism, and the engineering mycorrhizal fungi physically shields an inhibitor to construct cross-biosphere cooperative defense; the urease activity and the heavy metal removal rate are remarkably improved, meanwhile, the soil microbial diversity is maintained, and the soil specificity interference problem is solved.

The invention discloses a slope ecological restoration base material based on multistage synergistic solidification and a layered spray-seeding method, and belongs to the technical field of geotechnical engineering and ecological restoration. The invention provides a three-layer collaborative base material system and a dynamic construction process. In the base material, the bottom layer takes modified volcanic ash and nano silica sol as cementing materials, so that rock surface permeation and adhesion are enhanced; the middle layer adopts chitosan slow-release particles and gradient moisture content design, so that nutrient stage release and interlayer stress buffering are realized; the surface layer is combined with the 3D honeycomb-shaped biochar carrier and the degradable PLA grid, grass seeds and mycorrhizal fungi are synchronously loaded, and a physical-biological composite anchoring structure is formed. The construction method comprises high-frequency micro-vibration permeation, layered gradient spray-seeding and photosensitive non-woven fabric covering processes, and it is ensured that the base material is resistant to rainstorm scouring and rapid in vegetation covering. The method has both mechanical stability and ecological compatibility, is suitable for repairing the high and steep rock slope, and remarkably reduces the maintenance cost and the environmental pollution risk.

The invention provides inter-plant mycorrhiza nutrient transfer culture equipment which comprises a culture box body and an organic glass plate, an inner cavity of the culture box body is divided into a first culture chamber and a second culture chamber through the organic glass plate, the organic glass plate is a porous glass plate, and a white nylon net is pasted on one side of the porous glass plate. And the white nylon net is divided into a white nylon net with the aperture of 0.45 mu m and a white nylon net with the aperture of 37 mu m. The equipment is small in size, small in occupied area, cheap in material, convenient to assemble and suitable for pot culture experiments of large-batch samples, soil ions are allowed to pass through by means of 0.45 microns, ectogenic hyphae and the soil ions are allowed to pass through by means of a white nylon net of 37 microns, the soil ions, the ectogenic hyphae and root systems are allowed to pass through a nylon-free net, and the soil ions, the ectogenic hyphae and the root systems are allowed to pass through. Compared with the prior art, direct and indirect paths of nutrient transfer between plants can be distinguished and quantified, and the method is simple and easy to implement.

The invention relates to the technical field of agricultural biology, and discloses a microbial agent for preventing and treating fructus aurantii scab and a preparation method of the microbial agent. The microbial agent comprises the following raw materials in percentage by weight: 10-13% of paenibacillus mucilaginosus; 15%-20% of trichoderma sp. 7%-10% of paecilomyces lilacinus; 5%-8% of bacillus pumilus; 7%-9% of mycorrhizal fungi; 5%-8% of streptomyces; 5%-9% of a coptis chinensis extract; 4%-7% of a sophora flavescens extract; 2%-4% of realgar; 1%-3% of a rheum officinale extract; 10%-13% of soybean meal; 5%-8% of a water-soluble fertilizer; 7%-9% of potassium fulvate; 1%-3% of chitosan; 0.5%-1% of trace elements; 3%-8% of humic acid; the raw materials are subjected to microbial flora amplification culture, plant antibacterial component light-shielding mixing and low-temperature mixing to effectively protect the microbial activity of the microbial agent, and step-by-step operation can avoid damage of high temperature and mechanical shearing to microorganisms, so that the microbial activity of the microbial agent is effectively protected. And the loss of microbial active ingredients is reduced by vacuum drying and low-temperature granulation processes.

The invention discloses a growth-promoting rhizobacteria, a growth-promoting rhizobacteria microbial inoculum and application thereof, and belongs to the technical field of microorganisms, the growth-promoting rhizobacteria is Pseudomonas mohnii, the strain number is YQ142, the classification name is Pseudomonas mohnii, and the growth-promoting rhizobacteria is preserved in China General Microbiological Culture Collection Center on April 7, 2025, and the preservation number is CGMCC No.34110. The invention further discloses a preparation method of the growth-promoting rhizobacteria. The growth-promoting rhizobacteria has strong nitrogen fixation and phosphate solubilizing capabilities, can promote the growth of ryegrass, oat and corn, and improves the saline-alkali stress resistance and drought stress resistance of ryegrass.

The invention provides a cultivation method of ornamental small ginger, and relates to the field of plant cultivation, and the method comprises the following steps: tissue culture seedling cultivation: adopting a culture medium comprising a basic culture medium, a plant growth regulator, an organic additive, an antioxidant and trace elements for cultivation to obtain tissue culture seedlings. Performing potting cultivation: transferring the tissue culture seedlings into a potting container on which a mixed substrate is uniformly laid, and performing cultivation, wherein the mixed substrate comprises charcoal particles, modified zeolite particles, a mycorrhizal fungus inoculant, gelatin slow-release particles, fermented soybean meal and graphene modified cellulose; in the cultivation process, irrigation, fertilization and environment regulation and control are carried out regularly. According to the method, high-quality tissue culture seedlings are obtained by using a culture medium with specific components, and growth balance of ginger plants is adjusted and overgrowth of the plants is inhibited by adjusting a planting matrix and regulating and controlling a specific culture method, so that small potted ginger plants for ornamental use are obtained.

The invention relates to the field of soil remediation conditioners, and particularly provides a soil remediation conditioner for durian breeding as well as preparation and application of the soil remediation conditioner. The soil remediation conditioner is prepared from the following components in parts by weight: 35 to 45 parts of organic matter fertilizer, 18 to 22 parts of biochar, 22 to 28 parts of humic acid, 8 to 12 parts of vermiculite, 10 to 14 parts of perlite, 12 to 18 parts of shell powder, 6 to 10 parts of mycorrhizal fungi, EM bacteria and other mixed bacteria, 8 to 12 parts of wormcast, 4 to 6 parts of amino acid chelate solution, 8 to 12 parts of coco coir and 4 to 6 parts of adhesive. The specific surface area of the biochar is 32 m < 2 > / g, and the particle size is 40 meshes; the soil remediation conditioner is applied to durian planting soil remediation, has the effects of improving the physical structure of soil, improving the air permeability and water retention of the soil, increasing the fertility of the soil and providing rich nutrition for breeding and growth of durian seedlings, and has great application potential for the durian industry.

The invention discloses a method for guiding light rare earth surface mine degraded plant community restoration through combination of limonium bicolor, mycorrhizal fungi and tolerant earthworm cast, and the method utilizes a combination mode of limonium bicolor, mycorrhizal fungi and tolerant earthworm cast to guide and construct a plant community-soil fungi-soil ecosystem circulation system. According to the method, the existing plant community of the light rare earth mine is guided to recover by utilizing a combination method of combining dominant plant species with different mycorrhizal fungi and adding tolerance earthworm manure, mycorrhizal fungi and the earthworm manure fertilizer are added in the transplanting process of adult plants, so that the plant survival is promoted, and the stress resistance of light rare earth is enhanced at the same time; plant-mycorrhiza-wormcast fertilizer combinations aiming at different light rare earth elements are screened out, and the whole plant community of the mine is recovered and the content of the light rare earth elements in the soil is reduced through a plant community recovery method.

The invention discloses an ancient tree health state monitoring method based on digital twinning, and relates to the field of ancient tree health monitoring, and the method comprises the steps: collecting point cloud data and texture data of an ancient tree, and constructing an initial three-dimensional model of the ancient tree; a micro ion selective electrode array is deployed in an ancient tree root distribution area, Ca2 and K + ion flow velocity changes are detected through a constant potential rectifier, and the mycorrhizal symbiosis index is calculated in combination with soil temperature and humidity data; uploading the mycorrhizal symbiosis index to a digital twinborn body, and displaying the active state of a root system area in an initial three-dimensional model of the ancient tree in a three-dimensional thermodynamic diagram form; a 16-channel MOX gas sensor electronic nose array is installed on the surface of a trunk of an ancient tree, and volatile organic compound (VOC) concentration data of the ancient tree are collected. According to the invention, a micro electrode array with a 45-degree inclination angle is adopted to non-destructively monitor the ion flow velocity of a root system through the mycorrhizal symbiosis index detection unit, and a three-dimensional thermodynamic diagram is generated in combination with an inverse distance weighted interpolation algorithm, so that dynamic visualization of underground mycorrhizal activity is realized.

The invention discloses a mucilaginosa N15 and a method for promoting the germination of dendrobium huoshanense seeds. The mucilaginosa N15 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on October 27, 2025, and the preservation number of the strain is CGMCC No: 42290. The invention further discloses a method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15 and the method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15. The strain can be prepared into a high-activity microbial agent after activation and amplification culture, and the high-activity microbial agent is mixed with dendrobium huoshanense seeds and then sown in a greenhouse, so that the seed germination efficiency can be remarkably improved. By applying the method, the germination rate of the dendrobium huoshanense seeds can be increased to 60% or above, the mycorrhiza infection rate reaches 80% or above, the comprehensive cost is reduced by 30%, the seedling transplanting survival rate reaches 90% or above, and the seedling raising period is shortened to 12-15 months. The method is easy and convenient to operate, low in cost, high in transplanting survival rate and suitable for large-scale seedling raising and resource protection of dendrobium huoshanense, and has important economic and ecological values.

The invention belongs to the technical field of microorganism application, and particularly relates to a method for improving the growth and drought tolerance of camellia oleifera seedlings by using a microbial agent. The microbial inoculant is composed of a mycorrhizal inoculant and an European Stephania spp. Inoculant, the mycorrhizal inoculant is obtained by propagating a root soil mixture of the root of wild camellia oleifera, the European Stephania spp. Is Peziula ericae PE-1 obtained from the root system of wild camellia oleifera by adopting an endophytic fungus separation method, and the preservation number of the European Stephania spp. Is GDMCC NO: 64597. The method comprises the following steps: firstly, inoculating mycorrhizal inoculant at the roots of the camellia oleifera seedlings to promote the camellia oleifera seedlings to obtain the ability of rapid growth, and secondly, 15 days after the mycorrhizal inoculant is inoculated, irrigating the camellia oleifera seedlings with Eura petiole-free inoculant for 3-5 times to promote the camellia oleifera seedlings to obtain the ability of drought resistance. The method can well help the camellia oleifera to grow and resist drought stress, and is simple and easy to operate, low in investment and good in application prospect.

The invention relates to the technical field of cultivation of turfs, lawns or analogues, and discloses a vegetation blanket for slope protection and ecological restoration of drought and barren steep slopes and a use method of the vegetation blanket. Adults of the resuscitated plants, plant fibers and a water-retaining agent are prepared into a living resuscitated plant layer together, and the living resuscitated plant layer is subjected to yin cultivation before transplanting, so that normal planting can be carried out regardless of the orientation or existence of root systems, the plant adults are transplanted on a drought and barren abrupt slope in batches, and the survival rate of the plants is increased. A moisturizing and shading layer and a nutrient delayed release layer are adopted to maintain growth of the resuscitated plants, a layer of living soil with a certain water and fertilizer retention effect is formed, and the slope surface is preliminarily protected; after the water and fertilizer conditions on the slope surface are improved to meet the conventional plant growth requirements, coatings on the surfaces of the seeds in the seed delayed germination layer are degraded, so that the seeds germinate and grow at a proper time to realize water and soil conservation.

The invention discloses a method for controlling the growth condition of strawberry seedlings, and belongs to the technical field of strawberry seedling raising. The method for controlling the growth condition of the strawberry seedlings comprises the steps that illumination uniformity is adjusted, nutrient soil is prepared for strawberry seedling raising, and illumination uniformity adjustment comprises the steps that different numbers of LED lamp tubes are used for irradiating the growing strawberry seedlings, the illumination uniformity of strawberry seedling canopies is improved, and the energy efficiency ratio is increased; the preparation of the nutrient soil comprises the following steps: mixing and stirring the components of the nutrient soil to ensure the uniform distribution of the raw materials, then putting the nutrient soil into a planting pot, watering and wetting for strawberry seedling planting. The nutrient soil comprises the following components in parts by weight: 35-35 parts of leaf mold; 45 parts of the raw materials and 17 parts of vermiculite powder; 23 parts of perlite, and 23 parts of perlite; 30 parts of carbon nitride composite humic acid; 8 parts of the fertilizer and 5 parts of a mixed fertilizer; 7 parts, 1 part of mycorrhizal inoculant; and 3 parts. According to the method, the growth condition of the strawberry seedlings is cooperatively controlled by adjusting the illumination intensity and preparing the nutrient soil, so that the strawberry seedlings are developed in root system, thick and strong in leaf stems and free of plant diseases and insect pests, and the plant height and the leaf area of the strawberry seedlings are obviously increased after the seedling age is 30 days.

The present invention relates to an efficient method for artificially synthesizing Lactarius deliciosus mycorrhiza, which comprises the following steps: (1) Take the mycelium with a size of 1-3 mm at the connection of the stipe and cap of fresh Lactarius deliciosus fruit bodies, place it in a PDA medium, and conduct tissue culture at 20-25 °C for 20-30 days. Then, take the mycelium block and inoculate it onto the PDA medium, and continue to culture at 20-25 °C for 25-40 days. Subsequently, take the mycelium block again and inoculate it into a PDB medium for culturing for 30-50 days to obtain Lactarius deliciosus liquid spawn; (2) Transplant pine seedlings at a density of 6-15 seedlings per basket into a culture basket filled with mycorrhizal substrate, add the Lactarius deliciosus liquid spawn prepared in step (1) to the substrate 3-5 cm deep near the root of each seedling, and mycorrhiza can be formed after culturing for 10-14 days. The present invention scientifically screens the substrate and method for Lactarius deliciosus. By using the optimized medium formula and culture method, the mycelium of Lactarius deliciosus grows faster and the biomass of the obtained Lactarius deliciosus mycelium is larger; inoculating the plant roots with liquid spawn greatly improves the inoculation efficiency; multiple inoculated host plants are cultured in the same culture device, which greatly shortens the mycorrhiza formation time after inoculation and effectively improves the mycorrhiza synthesis rate; reducing the amount of peat soil in the mycorrhizal substrate reduces the proportion of mycorrhiza formed by miscellaneous bacteria and pine roots.

The invention provides a wheat nitrogen fertilizer application reducing and effect increasing method, and belongs to the technical field of biological fertilizers. According to the method, under the background of straw returning to the field, arbuscular mycorrhizal fungi and mushroom residue and excrement decomposed fertilizer are combined as a main body, then peat soil and bentonite are added for wheat cultivation, and application reduction and efficiency improvement of wheat nitrogen fertilizer are achieved; wherein the arbuscular mycorrhizal fungi and the mushroom residue and excrement decomposed fertilizer are combined to promote soil straw degradation and assist nitrogen transfer, the peat soil and the bentonite can improve the water and fertilizer retention capacity of the soil and promote hypha growth and reproduction, available nitrogen in the soil is increased, soil nutrient loss is reduced, and the nutrient absorption capacity is enhanced; further, nitrogen fertilizer absorption and utilization of wheat are improved, and dependence on chemical fertilizer is reduced.

The present invention relates to the field of microbial technology, and particularly to a Cladophialophora sp. CNOCC PP35 and its application in enhancing the photosynthesis of Paphiopedilum purpuratum. The Cladophialophora sp. CNOCC PP35 provided by the present invention is a strain of dark septate endophytic fungus isolated from the mycorrhizae of adult plants of Paphiopedilum purpuratum, with the preservation number of GDMCC NO: 65862. When co-cultured with the axenic seedlings of Paphiopedilum purpuratum, this strain can effectively promote the formation of chlorophyll a and total chlorophyll in the axenic seedlings of Paphiopedilum purpuratum, increasing by 122.35% and 98.42% respectively compared with the axenic control group, and can enhance the photosynthesis of the axenic seedlings of Paphiopedilum purpuratum; this strain can effectively promote the growth of the seedlings of Paphiopedilum purpuratum, with the fresh weight increasing by 127.78% compared with the control group, can realize the mycorrhization of the axenic seedlings of Paphiopedilum purpuratum, and shorten the artificial seedling raising time.

The invention relates to the technical field of crop planting. The invention provides an accurate fertilization and harvesting method for increasing yield and improving quality of sweet corn by nitrate nitrogen and rhizosphere symbiotic AM fungi. According to the technical scheme, the nitrogen and phosphorus compound fertilizer with the optimized proportion and AM fungi are synergistically used, so that accumulation of biomass of the mycorrhizal sweet corn and accumulation of nitrogen elements, phosphorus elements, soluble sugar content and amino acid content in sweet corn kernels are effectively promoted, and then the yield of the sweet corn and the kernel quality are improved; the utilization rate of nitrogen and the utilization rate of phosphorus are increased by 29.54% and 19.43% respectively by the AM fungus mycorrhizal corn. In addition, the time from planting to harvesting of the sweet corn ranges from 8 weeks to 9 weeks, the protein nutrition of the sweet corn is relatively best in the harvesting period, and the grain yield is relatively highest.

The invention relates to the technical field of forest seedling raising machinery, and discloses a seedling raising and sowing device capable of realizing synchronous application of a forest mycorrhizal agent along with seeds, and by driving two groups of discharging mechanisms of independent storage assemblies for respectively containing forest seeds and mycorrhizal agents to synchronously rotate, the discharging quantity of the two materials and the advancing speed of the device are completely rigidly bound; on the other hand, it is guaranteed that the discharging proportion is constant and accurately matched with the advancing distance from the mechanical structure, finally, the materials are fully mixed through spiral mixing blades in a discharging pipe and then synchronously fall out, and the mycorrhizal agent and seeds are synchronously applied along with the seeds in the same hole; and intermittent extrusion is formed on an extrusion plate on the sliding ring, the sliding ring is driven to drive a material baffle in the storage barrel to synchronously perform circumferential reciprocating turning along with the walking speed in cooperation with rebounding reset of a reset elastic piece in the arc-shaped pipe, and the materials are continuously homogenized to avoid blocking of a discharging channel due to caking and adhesion.

The present application relates to the technical field of fruit tree propagation, in particular to a pear, plum or apricot germplasm resource nursery strip soil cutting method in cold and arid regions, first, one-year-old branches are selected, after disinfection, soaked in rooting mud containing humic acid and growth regulator, then, a cubic soil nub is dug in the rhizosphere of the mother plant, wrapped with non-woven fabric, injected with water-retaining agent, sprayed with humic acid potassium and chitin mixed solution, and inoculated with mycorrhizal fungi, finally, a planting hole is dug according to the set plant spacing, a straw root protection cover is set, the strip with soil is planted, water is poured thoroughly and slow-release fertilizer is buried. The technology effectively protects the root system and promotes rooting through comprehensive treatment, ensuring the rapid and high-quality construction of the resource nursery under the harsh conditions in the cold and arid regions. Through the triple root protection soil cutting technology, the survival rate of pear, plum, apricot and other deciduous fruit tree germplasm resource strips in cold and arid regions is increased from less than 60% of the traditional method to more than 85%.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.

This invention provides the application of mycorrhizal fungi in regulating carbon allocation in Dendrobium officinale plants, belonging to the field of orchid mycorrhizal symbiosis technology. The invention includes the following steps: preparing a mycorrhizal fungal inoculant, wherein the mycorrhizal fungi are one or both of *Cladosporium halophilum* or *Dendrobium nobile*; soaking the roots of Dendrobium officinale tissue culture seedlings in the mycorrhizal fungal inoculant, and then transplanting them into a culture medium for cultivation. This invention, by inoculating the roots of Dendrobium officinale with mycorrhizal fungi, promotes mycorrhizal symbiosis and growth of Dendrobium officinale, while also inducing the expression of certain specific genes in Dendrobium officinale, activating a series of secondary metabolic pathways, thereby causing changes in the carbon allocation of the aboveground parts and roots of Dendrobium officinale seedlings, further influencing and regulating the biosynthesis of Dendrobium officinale polysaccharides, which is beneficial for further elucidating the molecular regulatory mechanism of mycorrhizal symbiosis on sugar metabolism in Dendrobium officinale.

The invention relates to a tool and a method for separating, sterilizing and filtering arbuscular mycorrhizal fungi monospores. The device comprises a microoperation module and a multi-stage filtering module which work cooperatively. Wherein the microoperation module comprises a microsuction device and a filtering module, the filtering module comprises an upper filtering section and a lower filtering section, the upper filtering section comprises a first cylindrical section, a second cylindrical section and a third cylindrical section which are coaxially arranged and sequentially connected, and a filter screen is arranged at the end of the third cylindrical section; the lower filtering section comprises a fourth filtering section and a fifth filtering section which are coaxially arranged and connected with each other, the fourth filtering section is used for sleeving the third filtering section, and the fourth filtering section and the fifth filtering section jointly form a filter for filtering spores extracted by the micro-suction device. The tool has the innovativeness that the tool can effectively solve the problem of single separation of AMF fungal spores, and loss of the spores is reduced in the sterilization process.

The invention relates to the technical field of fertilization, in particular to an edible mushroom planting automatic fertilizer applicator which comprises a fertilization moving vehicle, a plurality of moving wheels are arranged at the bottom of the fertilization moving vehicle and used for driving the fertilization moving vehicle to move, and a plurality of fertilizer storage cylinders are mounted on the upper end face of the fertilization moving vehicle and used for storing fertilizer in the fertilizer storage cylinders. A plurality of supporting frames are slidably installed on the upper end face of the fertilization moving trolley, the supporting frames are arranged on the sides, away from the fertilizer storage barrel, of the supporting frames, and feeding needle heads are arranged on the sides, away from the fertilizer storage barrel, of the supporting frames and can move up and down on the side of the fertilization moving trolley so as to penetrate into edible fungus soil; the edible mushroom soil is directly fertilized, the feeding needle head can penetrate into the edible mushroom soil, and the fertilizer in the fertilizer storage barrel is introduced into the edible mushroom soil, so that the fertilizer can be in direct contact with mycorrhiza, the combination of the mycorrhiza and fertilizer nutrients is ensured, and the self growth of edible mushrooms is facilitated.

The utility model discloses a device convenient to separate soil mycorrhizal spores, and relates to the technical field of fungus cultivation devices, the device comprises a base and an adjusting assembly, the adjusting assembly is arranged at the top of the base, the adjusting assembly comprises an adjusting frame, and the adjusting frame is fixedly connected to the top of the base. According to the device convenient for separating the soil mycorrhizal spores, a worker can quickly and accurately adjust the vibration frequency according to the characteristics of a soil sample by installing the adjusting assembly, so that the operation convenience is remarkably improved, and the separation requirements of different soil samples can be better met by adjusting the vibration frequency; separation is ensured under the optimal vibration condition, the separation efficiency and spore integrity are improved, damage to spores in the separation process can be reduced easily through accurate vibration frequency adjustment, it can be ensured that the spores are subjected to sufficient vibration to be separated, meanwhile, too large mechanical stress is avoided, the separation quality is improved, and the spore separation efficiency is improved. Therefore, the separation efficiency is prevented from being influenced.

The invention provides bacillus altitudinis CL6 with a morchella esculenta continuous cropping resisting effect and application of the bacillus altitudinis CL6, and belongs to the technical field of microorganisms. The bacillus altitudinis CL6 with a continuous cropping resisting effect on morchella is separated from morchella rhizosphere soil and is preserved in the China Center for Type Culture Collection on March 18, 2025, and the preservation number is CCTCC NO: M 2025516. The bacillus altitudinis CL6 disclosed by the invention can be used as an auxiliary added beneficial microbial agent in the cultivation production of the morchella to resist the continuous cropping harm of the morchella, and finally, the cultivation stability of the morchella is improved. The strain provided by the invention can promote the continuous cropping resistance effect of morchella esculenta and increase the yield of morchella esculenta. And a green and efficient anti-continuous cropping biological solution is provided for the morchella esculenta cultivation industry.

The invention discloses a quality control method in mycorrhizal edible mushroom seedling production, and belongs to the technical field of forest seedling production. The method comprises the steps of strain detection, sterilized planting matrix detection, planting environment sterilization and disinfection treatment, temperature and humidity control, personnel control, nutrition supply and target seedling detection. According to the quality control method in mycorrhizal edible mushroom seedling production, quality control is carried out on multiple aspects in seedling cultivation production at the same time, particularly nutrient supply and target seedling detection means are adopted, the quality of mycorrhizal edible mushroom seedlings is improved, the adaptability of the mycorrhizal edible mushroom seedlings in the actual cultivation process is improved, and the yield of the mycorrhizal edible mushroom seedlings in the actual cultivation process is increased. The method is simple to operate, systematic, easy to popularize and suitable for actual production. The method is suitable for production of mycorrhizal edible mushroom seedlings.

The present invention belongs to the field of composite materials technology, specifically relating to an edible mycorrhizal glycoconjugate-graphene conductive film and its preparation method. This invention uses chitosan-glucan complex (CGC), a root glycoconjugate extracted from the mycorrhizae of Flammulina velutipes, as raw material. Extracting CGC from the mycorrhizae requires only mild acid and alkali treatment to remove components such as proteins, minerals, and lipids, while also minimizing damage to the CGC and improving its purity. The prepared CGC, when used as a dispersant, improves graphene agglomeration. Compared to graphene without CGC, at the optimal concentration of CGC added, the median particle size decreased by 43.11%, and the absolute value of the zeta potential increased by 160.07%. The addition of CGC to graphene significantly enhances its dispersibility, improving the tensile strength and conductivity of the CGC-graphene film.

The invention relates to the technical field of solid waste resourceful treatment, and discloses coal gangue-based improved soil and a preparation method thereof. The coal gangue-based improved soil comprises the following raw materials: coal gangue, sandy soil and a fermentation inoculant, the fermentation bacterial agent is prepared from bacillus subtilis SHBCC D11446, arbuscular mycorrhizal fungi SHBCC D24696, trichoderma viride SHBCC D10197 and bacillus velezensis GS02, and the preservation number of the bacillus velezensis GS02 is CCTCC (China Center for Type Culture Collection) NO: M 2025531. Phosphorus, potassium, nitrogen and organic matters in the coal gangue can be fully utilized, the prepared coal gangue-based improved soil has high nutrient substance content and low effective state heavy metal content, and meanwhile, the situation that the alkalinity and the salt content of the improved soil are greatly improved due to introduction of the coal gangue can be avoided; the improved soil can effectively promote plant growth.

The invention provides boletus nigrum G8 as well as a culture method and application thereof, and belongs to the technical field of edible mushrooms. The preservation number of the boletus nigrum G8 is CGMCC (China General Microbiological Culture Collection Center) No.20233. The invention also provides a corresponding culture method of the boletus nigrum G8 and application of the boletus nigrum G8 in culture of boletus nigrum mycorrhizal seedlings and potting. Compared with other strains, the boletus brownii G8 has the advantages that the growth speed is higher, and the obvious competitive advantage and application value are achieved; sporocarp can be formed in a short time through potting, and continuous mushroom production is achieved.

The invention discloses pseudomonas chlororaphis subsp. Aurantii, a complex microbial inoculant and application of the complex microbial inoculant in relieving continuous cropping obstacles of apples, and belongs to the technical field of agricultural microorganisms. According to the application disclosed by the invention, a strain of pseudomonas chlororaphis subsp. Aurantiaca S74 is separated from mycorrhiza rhizosphere, and the strain has an antagonistic effect on various pathogenic bacteria causing continuous cropping obstacles of apples; the strain has the capabilities of producing protease, secreting siderophores, dissolving inorganic phosphorus, fixing nitrogen and producing ammonia gas. When the strain S74 is applied to apple continuous cropping soil in cooperation with AMF, the number of bacteria in the soil can be increased, the number of pathogenic bacteria can be reduced, plant growth can be promoted, the chlorophyll content of overground leaves of plants can be increased, meanwhile, colonization of AMF in the continuous cropping soil and plant root systems can be promoted, and the strain S74 can relieve apple continuous cropping obstacles in cooperation with AMF. The application method of the strain S74 belongs to the field of biological prevention and control, and provides technical support for comprehensive green prevention and control of apple continuous cropping obstacles.