55 results about "Root disease" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas monteilii BDLZ-3 and application thereof in relieving continuous cropping obstacles of crops. The invention discloses a strain of pseudomonas monteilii BDLZ-3, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.34349. The pseudomonas monteilii BDLZ-3 is separated and screened from the nature for the first time, phenolic acid autotoxic substances generated in the ginseng continuous cropping obstacle process can be efficiently degraded, the degradation rate of benzoic acid can reach 95.36%, various phenolic acids such as vanillic acid, cinnamic acid, palmitic acid and gallic acid can be effectively degraded, and the degradation spectrum is wide; when the pseudomonas monteilii BDLZ-3 is applied in a field, the content of benzoic acid in soil can be effectively reduced, meanwhile, the occurrence of ginseng root diseases can be controlled to a certain extent, the prevention and treatment effect reaches 41.23%, the growth indexes such as root length and root thickness are remarkably improved, and the yield increasing effect is achieved.

The invention relates to a microbial strain and application thereof, in particular to bacillus brevis, a microbial agent prepared from the bacillus brevis and application of the microbial agent. The invention provides the cold-resistant bacillus subtilis which has multiple functions of generating capsular polysaccharide, being high in salt resistance and cold resistance, promoting growth and disease resistance of plants in saline-alkali soil and the like. The bacterial strain can generate capsular polysaccharide, experiments prove that the bacterial strain has high capabilities of dissolving phosphorus, fixing nitrogen and producing siderophore, IAA, ACCD and cytokinin, and has strong antagonistic action on fusarium, curvularia and microplasmodium, and the generated capsular polysaccharide can promote salt tolerance of plants, so that the yield of the plants is increased, and the yield of the plants is increased. The bio-fertilizer is suitable for production of multifunctional bio-fertilizers with disease prevention, growth promotion, salt and alkali resistance, cold resistance and the like, is applied to production of saline-alkali soil grain crops, pasture and greenhouse commercial crops, has remarkable effects of promoting cold damage resistance, root development, prevention and control of root diseases, improvement of crop yield and the like, can reduce the use of chemical fertilizers and pesticides, and has a wide application prospect.

The invention discloses biocontrol marine source penicillium and application thereof, and relates to the technical field of microorganisms. The biocontrol marine source penicillium is named as Penicillium sp.DSF059 and is preserved in the China Center for Type Culture Collection, and the preservation number of the biocontrol marine source penicillium is CCTCC NO: M 20232310. The invention discloses biocontrol marine-source penicillium. The biocontrol marine-source penicillium has effects on rubber fusarium wilt pathogenic bacteria, coconut gray leaf spot pathogenic bacteria, wheat scab pathogenic bacteria, cucumber phytophthora root rot pathogenic bacteria, avocado phytophthora root rot pathogenic bacteria, rubber anthracnose pathogenic bacteria, mango anthracnose pathogenic bacteria, sisal zebra stripe pathogenic bacteria, rubber red root pathogenic bacteria, bacillus subtilis and bacillus subtilis. The invention provides a new direction for the prevention and treatment of the tropical crop pathogenic bacteria, and has a very good development and application prospect, and the tropical crop pathogenic bacteria such as the pathogenic bacteria of the rubber brown root disease, the coffee anthracnose pathogenic bacteria, the stylosanthes guianensis anthracnose pathogenic bacteria and the banana anthracnose pathogenic bacteria can be prevented and treated.

The invention provides a preparation method and device of hydrogen-oxygen interface nano-micron bubble water for agricultural production, and the method comprises the following steps: S1, filtering raw water for multiple times, and purifying the raw water into purified water; s2, electrolyzing the purified water to decompose the purified water into hydrogen and oxygen; s3, the hydrogen and the oxygen respectively pass through an interface nano-micron bubble generation device to form interface nano-micron bubble water; s4, the interface nano-micron bubble water is conveyed to a ceramic activation device, and the activity of the interface nano-micron bubble water is improved; s5, mixing the activated interface nano-micron bubble water with an external water source to form irrigation water. Raw water is purified into purified water through multiple times of filtration, impurities and harmful substances in water are effectively removed, and a purer irrigation water source is provided for plants. Interface nano-micron bubble water formed by hydrogen and oxygen generated by electrolysis is rich in active oxygen and molecular hydrogen, so that the oxygen content of soil and the growth of soil microorganisms are improved, healthy growth of plant roots is promoted, root diseases caused by fungal viruses are reduced, oxidation and inflammation are resisted, and thus healthy growth of plants is promoted.

The invention discloses a method for visually detecting grape black root bacteria Ilyonectria sp. Based on RPA-CRISPR / Cas12a and application of the method for visually detecting the grape black root bacteria Ilyonectria sp. Based on the RPA-CRISPR / Cas12a. According to the method, an RPA specific amplification primer, a CrRNA probe and an ssDNA probe are adopted; according to the RPA specific amplification primer, an HIS gene is used as a specific recognition target, and specific amplification can be carried out on the grape black root pathogen Ilyonectria. The method for detecting the grape black root pathogenic bacteria has high specificity and sensitivity, is simple, convenient and rapid in operation flow, and is suitable for rapid screening and preliminary diagnosis of target pathogens in a field environment or a basic detection scene.

The invention belongs to the technical field of cortex acanthopanacis planting, and particularly relates to an under-forest cortex acanthopanacis water and fertilizer integrated planting method. The invention relates to an under-forest cortex acanthopanacis water and fertilizer integrated planting method, which comprises the following steps: S1, selecting a low-hill mountain land with the altitude of 200-400m and the gradient of lower than 15 degrees according to the tree age of 3-5 years and the canopy density of 0.25-0.35; s2, drip irrigation pipes are buried after soil preparation, then cortex acanthopanacis cutting slips are cut, clear water is poured thoroughly, and microbial water and fertilizer are dropped through the drip irrigation pipes in the subsequent planting process; wherein the microbial water fertilizer is formed by mixing bacillus safensis fermentation liquor and bacillus megatherium fermentation liquor. According to the method, in the under-forest cortex acanthopanacis integrated planting process, microbial water and fertilizer are subjected to drip irrigation, the prevention and treatment effect on cortex acanthopanacis root diseases can be effectively prevented and improved, and development of under-forest cortex acanthopanacis integrated planting is promoted.

The present invention relates to the field of sampling technology, and discloses a ginger root sampling device for root disease research, comprising a base and a mounting platform arranged above the base, a limiting plate 1 is fixed on a side of the mounting platform away from the base, a limiting plate 2 sliding on the mounting platform is arranged on one side of the limiting plate 1, the limiting plate 1 is provided with an opening, a cutting blade is arranged on the side of the limiting plate 1 close to the limiting plate 2, a clamp for fixing the ginger root is arranged on the mounting platform, and a sampling assembly is arranged on the base. The present invention can quickly sample the ginger root, is easy to use, has high safety performance, can adaptively regulate the sampling device, can sample different positions of the ginger root, thereby facilitating the planting personnel to study the diseases of the ginger root, and has high working performance.

The application provides a plant cultivation method and system combined with root disease and pest monitoring, relates to the technical field of disease and pest monitoring, and the method comprises the following steps: acquiring a target plant planting time point and a monitoring time interval, and constructing multiple monitoring time points; at each monitoring time point, collecting the soil temperature, soil humidity, soil oxygen and root rot fungus quantity of a target area, and acquiring a root rot fungus predicted quantity of a next monitoring time point; comparing the root rot fungus quantity collected at the current monitoring time point with the root rot fungus predicted quantity of the last monitoring time point, and obtaining an accumulated prediction error of the current monitoring time point; correcting the root rot fungus predicted quantity of the next monitoring time point according to the accumulated prediction error, and obtaining a corrected root rot fungus quantity; and when the corrected root rot fungus quantity is greater than or equal to a preset root rot fungus threshold, beneficial bacteria are put into the target area. The technical problems of low root rot fungus monitoring precision and lagging prevention and control measures in the prior art are solved.

The application discloses a bacillus velezensis A13 wettable powder and application thereof, and belongs to the technical field of biological pesticides. The application aims to solve the problems of limited prevention and treatment effect of existing seed treatment, high labor intensity of foliar spraying and short effective period. The wettable powder comprises a mother powder prepared from fermentation liquor and diatomite, and a dispersant, a wetting agent, a stabilizer and a protective agent, wherein the total addition amount of the dispersant and the wetting agent is 5%-14% of the mass of the mother powder, the addition amount of the protective agent is 0.5%-2% of the mass of the mother powder, and the addition amount of the stabilizer is 1%-3% of the mass of the mother powder. The application can be used for preventing and treating root diseases caused by panax quinquefolius rust rot fungus and leaf diseases caused by panax quinquefolius alternaria leaf spot fungus, and promoting plant growth.

The invention relates to the technical field of agriculture, in particular to a pelleting formula of isatis tinctoria seeds capable of resisting root diseases and promoting rooting. The formula comprises the following components: isatis tinctoria seeds, an antifungal agent, a rooting accelerant, a humectant, a nutrition additive and an adhesive. The pelleting formula is prepared by the following steps: firstly, uniformly mixing isatis tinctoria seeds with an antifungal agent; adding a rooting accelerant, and continuously stirring; adding the humectant, and fully mixing; adding a nutrition additive, and uniformly mixing with the seeds; adding an adhesive, and continuously stirring; feeding the mixture into a granulator for granulation to form seed pellets with the diameter of 3-5mm; and drying the prepared seed pellets, and packaging after quality inspection. According to the isatis tinctoria seed pelleting formula capable of resisting root diseases and promoting rooting provided by the invention, the disease resistance and rooting rate of the seeds are remarkably improved by optimizing formula components and a preparation process.

The invention provides a method for preparing a traditional Chinese medicine composition for treating lumbar disc herniation accompanied by radiculopathy. The traditional Chinese medicine composition comprises, by weight, 5-10 parts of peach kernel, 3-10 parts of safflower, 10-15 parts of raw rehmannia, 5-15 parts of angelica, 5-12 parts of red peony root, 3-9 parts of chuanxiong, 5-15 parts of salvia miltiorrhiza, 3-10 parts of corydalis, 3-9 parts of notopterygium root, 3-9 parts of angelica pubescens, 4.5-9 parts of siler, 1.5-3 parts of processed chuanwu, 5-10 parts of tuckahoe, 9 parts of astragalus root, 1.5-10 parts of tuckahoe, 1.5-2 parts of processed chuanwu, 5-10 parts of tuckahoe, 9 parts of astragalus root, 1.5-2 parts of processed chuanwu, 1.5-10 parts of tuckahoe ... The traditional Chinese medicine composition of the present invention has the effects of reducing spinal canal pressure to reset the protrusion, relieving nerve compression, repairing damaged annulus fibrosus, and achieving the treatment of lumbar disc herniation with radiculopathy.

The utility model discloses a multifunctional urban landscaping plant planting box, and particularly relates to the technical field of plant planting devices.The multifunctional urban landscaping plant planting box comprises a box body, a containing groove is formed in the upper end face of the box body, a planting plate is fixedly connected to the inner surface wall of the containing groove, and a water supplementing structure is arranged on the outer side of the planting plate; the water supplementing structure comprises a water storage tank arranged in the containing groove. The planting box has a water replenishing function by arranging the water replenishing structure, when the planting box is used, automatic on-demand water replenishing for green plants is achieved, water can be replenished in time sensitively and accurately according to the actual requirements of the plants, the root systems of the plants are soaked in the environment with the appropriate humidity all the time, and the planting box has the water replenishing function. The problem that growth is blocked due to water shortage is effectively avoided, the hidden danger of root diseases caused by excessive water is avoided, plants can thrive in a healthy and stable environment, it is guaranteed that the growth situation of the plants is good, and the long-term attractiveness and ecological stability of the whole greening landscape can be maintained.

The invention relates to the technical field of crop irrigation, in particular to a self-adjusting pressure drip irrigation system based on soil moisture content real-time feedback. According to the self-adjusting pressure drip irrigation system based on real-time feedback of the soil moisture content, the technical problem that in the prior art, a drip irrigation system cannot accurately control the irrigation duration is solved. A self-adjusting pressure drip irrigation system based on real-time feedback of soil moisture content comprises a soil moisture content sensor used for acquiring the soil moisture content; the soil moisture content is obtained in real time through the soil moisture content sensor, and the current soil moisture condition can be accurately known. In a key period of crop growth, such as a flowering and fruiting period, which is extremely sensitive to water demand, the system can accurately control irrigation according to real-time soil moisture content data, so that crops can obtain proper water in each growth stage, growth limitation caused by water shortage or root diseases caused by excessive water are avoided, and the crop quality is improved. Therefore, healthy growth of crops is guaranteed, and yield and quality are improved.

The invention relates to a bacterium for preventing and treating grape root diseases and application thereof. The bacillus velezensis GP50 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.32909 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. The viable bacteria and metabolites of the strain have efficient prevention and treatment effects on grape root diseases. The strain is separated from grape rhizosphere soil, the fermentation culture time is short, the production cost is low, the control effect is further improved when the strain is matched with a chemical agent for use, and the strain has a good application prospect.

The invention provides a fertilizer-pesticide double-effect biological organic preparation special for lily and a use method thereof, and belongs to the technical field of lily planting. The fertilizer and pesticide double-effect biological organic preparation special for lily comprises the following components in parts by mass: 30-50 parts of a soybean meal extract, 20-30 parts of a seaweed extract, 10-20 parts of humic acid, 8-12 parts of sophocarpidine, 5-10 parts of carvacrol and 3-7 parts of a complex microbial inoculant. The biological organic preparation prepared by the invention can obviously improve the emergence rate, the cut flower rate and the first-class flower rate of the lily, reduces the occurrence of leaf and root diseases, realizes the purpose of double effects of fertilizer and pesticide, and has a wide application prospect.

The invention belongs to the technical field of crop disease and pest control, and particularly relates to a reagent for improving resistance of tobacco to phytophthora nicotianae and application. The reagent is a mixed solution of a copper sulfate solution and nano carbon sol. The reagent provided by the invention is green and environment-friendly, and has no hidden danger of environmental pollution; through external application, the resistance of tobacco to phytophthora nicotianae can be obviously enhanced, and the occurrence of tobacco root diseases is reduced. Compared with existing biological prevention and control methods and the like, the method for preventing and controlling phytophthora nicotianae adopts a solution root irrigation mode to prevent and control phytophthora nicotianae, has the potential advantages of being easy and convenient to operate, low in cost, rapid in effect taking, remarkable in effect, free of pollution, free of public nuisance, capable of achieving in-situ treatment and the like, and has the application prospect of application and popularization in the tobacco field.

The invention belongs to the technical field of traditional Chinese medicinal material cultivation, and particularly relates to an under-forest traditional Chinese medicinal material ecological efficient cultivation method which comprises the following steps: S1, forest land preparation: one month before planting, applying a decomposed organic fertilizer to the surface of the forest land, and deeply ploughing soil; s2, seedling planting: planting fructus alpiniae oxyphyllae and radix fici simplicissimae according to an interplanting mode; s3, timely topdressing: topdressing decomposed organic fertilizer after 3-5 months of planting of the fructus alpiniae oxyphyllae and 2-3 months of planting of the radix fici simplicissimae; s4, field management. According to the ecological efficient cultivation method for the under-forest traditional Chinese medicinal materials, through scientific forest land selection, variety matching, fertilization management and other measures, the yield and quality of the fructus alpiniae oxyphyllae and the radix fici simplicissimae can be remarkably improved, meanwhile, the occurrence rate of root diseases of the fructus alpiniae oxyphyllae and the radix fici simplicissimae can be reduced, the usage amount of chemical pesticides and chemical fertilizers can be reduced, environmental pollution can be reduced, and the economic benefit is increased. The safety of the traditional Chinese medicinal materials is improved. In addition, by using the decomposed organic fertilizer, the soil quality can be improved, the ecological environment of the forest land is protected, and sustainable development of the under-forest traditional Chinese medicinal material industry is achieved.

The present invention relates to the technical field of microorganisms and bio-organic fertilizers, and specifically discloses a bio-organic fertilizer for preventing and treating wasabi root diseases and its application. The bio-organic fertilizer comprises the following components: Component A: Bacillus velezensis agent with a deposit number of CCTCC NO: M 2022923 and Trichoderma yunnanensis agent with a deposit number of CCTCC NO: M 2025645; Component B: soybean meal, vinegar dregs, biochar, wollastonite powder, and allicin. The bio-organic fertilizer of the present invention can increase the content of growth hormones such as salicylic acid (SA), jasmonic acid (JA), and ethylene (ET), and the phytoalexin content in the wasabi root system, as well as the activity of chitinase and β-1,3-glucanase, and has an effect on the growth of stem mold of wasabi ( Phoma wasabiae Yokog) infection and carrot soft rot caused by Pectobacterium carrot subspecies ( Pectobacterium carotovorum subsp .carotovorum, It has a good control effect on soft rot caused by Pcc) infection.

The present application discloses a rape clubroot prevention and control fertilizer and a preparation method thereof, and relates to the technical field of rape clubroot prevention and control. A rape clubroot prevention and control fertilizer, by weight, includes the following components: 20-30 parts of a nitrogen source, 18-23 parts of a phosphorus source, 17-22 parts of a potassium source, 20-30 parts of slaked lime, 0.3-0.8 parts of magnesium sulfate, 0.5-1.0 parts of sodium borate, 10-15 parts of humus, and 0.3-0.8 parts of a growth regulator. The rape clubroot prevention and control fertilizer of the present application is rich in a variety of nutrients and organic substances. Through the coordinated use of each component, the soil ecological environment can be effectively regulated, the root growth of rape can be promoted, and the cold and disease resistance of rape can be enhanced, thereby effectively preventing the occurrence of rape clubroot and increasing rape yield.

The invention discloses a compound microbial agent and application thereof in prevention and treatment of tobacco rhizome diseases, and relates to the technical field of biological prevention and treatment. The compound microbial agent provided by the invention comprises a compound microbial agent 1 and a compound microbial agent 2, the compound microbial agent 1 comprises bacillus methylotrophicus, streptomyces albidoflavus and bacillus halotolerant, and the compound microbial agent 2 comprises streptomyces oryzae and bacillus caramei. Through verification, the compound microbial agent provided by the invention can promote agronomic characters of tobacco plant height, stem circumference, leaf number, maximum leaf length, maximum leaf width and the like, can also effectively prevent and treat tobacco rhizome diseases including tobacco root rot, tobacco black shank, tobacco bacterial wilt, tobacco sclerotinia rot and the like, and has application value in the field of tobacco planting.

The invention discloses a tomato seedling raising method capable of resisting root diseases. The tomato seedling raising method capable of resisting the root diseases comprises the steps of pretreatment, pretreatment, seedling raising and transplanting. The tomato seedling cultivated by the method is a transgenic chimeric plant, the root system of the tomato seedling is a transgenic root tissue with a root disease resisting effect, and the overground stem, leaf, flower and fruit part is a native tissue which keeps the character characteristics of the original tomato variety, so that the method integrates the advantages of grafting and genetic engineering breeding, and has the advantages of simple and convenient operation of grafting, high yield and the like. The method also has the advantage of wide sources of disease-resistant DNA fragments of genetic engineering breeding, and also overcomes the limitation of grafting adaptability between scions and stocks in a grafting technology and the problem of food safety in genetic engineering breeding.

This invention relates to a new and distinct variety of strawberry plant named ‘CIVH413’. The new June bearing strawberry variety named ‘CIVH413’ is primarily adapted to the climate and growing conditions of continental climate and is characterized by a rustic plant with upright habit and visible fruit easy to collect; the vigour is medium. The new strawberry has a high productivity, consistent during all the picking season; in particular is tolerant to the most common leaf and root diseases. The fruits have conical, attractive and very regular shape with orange-red colour; the size is big and consistent during all the time of picking. The firmness is high with a very good shelf life. The flavour is very good, balanced and aromatic.

The present invention provides a novel nucleic acid molecule encoding a polypeptide that can confer resistance to pathogens, especially the "Beet Necrosis Yellow Vein Virus" (BNYVV), in plants, especially in plants of the genus Beta expressing the polypeptide, and also provides preferred nucleic acid molecules encoding the RZ-3 gene of Beta maritima, as well as derivatives and homologues thereof. Further aspects of the invention include vectors, transgenic plant cells, transgenic plants, transgenic plants and methods of making them, and methods of identifying nucleic acid molecules that confer resistance.

The invention discloses a special organic fertilizer for tobacco planting, which relates to the technical field of organic fertilizers for tobacco, and is characterized in that the special organic fertilizer for tobacco planting comprises the following components in percentage by mass: 10-15% of oil cake, 30-35% of humic acid, 25% of straw, 10-15% of charcoal dust and compound bacteria, and the compound bacteria comprise bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens. The organic matter content of soil is increased, the activity of beneficial microorganisms is enhanced, flue-cured tobacco root system diseases are reduced, the soil air permeability is enhanced, and the tobacco growth trend is obvious; after being applied, the product is safe to personnel, soil environment and crop planting, the produced agricultural products are safe, and the numerical value can be stably reduced for a long time.

The invention provides a method and system for detecting root diseases and insect pests of rhizome plants by using an electronic nose in a laboratory, and relates to the technical field of plant disease and insect pest detection.The method comprises the steps that plant samples with known diseases and insect pests are placed in a closed container to release VOCs, and after the set gas release time is reached, gas is divided into an experimental group and a detection group; acquiring a gas concentration accurate value under a reference environment condition by using a gas chromatograph-mass spectrometer; collecting detection group data at different temperatures, humidity and air inlet rates through an electronic nose, constructing a relation curve of environmental factors and detection values, and calculating the influence intensity of each factor on a detection result; and finally, constructing a fitting function, correcting the gas concentration detection value of the to-be-detected sample, and judging the grade of plant diseases and insect pests through a deep learning model according to the corrected concentration. According to the system, rapid, lossless and accurate detection of root diseases and insect pests is realized.

The utility model discloses a barrier type tobacco seedling growing device for preventing tobacco rhizome diseases, which comprises a shaping outer frame, a sealing mounting sleeve plate is fixedly mounted on the inner side of the shaping outer frame, a plurality of positioning seats are mounted on the inner side of the sealing mounting sleeve plate, adjusting liquid guide rods are mounted at the upper ends of the positioning seats, and the adjusting liquid guide rods are mounted on the outer side of the shaping outer frame. A positioning plate is mounted above the sealing mounting sleeve plate, a culture soil isolation plate is mounted above the positioning plate, and a plurality of automatic liquid supplementing mechanisms are mounted on the inner side of the culture soil isolation plate; the automatic liquid supplementing mechanism comprises a mounting sleeve, a guide sleeve is fixedly mounted on the inner side of the mounting sleeve, and a liquid supplementing head is mounted at the upper end of the guide sleeve. Compared with the prior art, the barrier type tobacco seedling cultivation device has the advantages that the barrier type cultivation is carried out on the tobacco seedlings, nutrition supply of single tobacco seedlings can be achieved, the supply quantity can be independently and accurately controlled conveniently, and generation and infection of tobacco rhizome diseases are effectively prevented.

The invention relates to a planting method for interplanting stephania erecta in a rubber forest, and belongs to the technical field of agricultural planting. The invention provides a planting method for interplanting stephania erecta in a rubber forest. The planting method comprises the steps of forest land selection, soil improvement, stephania erecta planting and cultivation management. According to the method, the stephania erecta is interplanted in the rubber forest, the blank forest land in the rubber forest is fully utilized, meanwhile, the problem that environmental lighting needs to be set deliberately for stephania erecta planting is solved, the stephania erecta and the rubber trees are harmonious and symbiotic through the planting method, and the yield of the stephania erecta is increased. The rattan trimmed from the stephania erecta is used as green manure to be applied to the rubber tree, so that the rubber yield of the stephania erecta can be increased, meanwhile, the weight of stephania erecta tuberous roots can be increased by applying decomposed leaves of the rubber tree as fertilizer to the stephania erecta, the number of root-knot nematodes in forest land can be remarkably reduced through planting of the stephania erecta, and root diseases of the rubber tree are reduced.

The invention discloses a vigna unguiculata pest and disease damage biosafety prevention and control method based on screening and identification technology research. The method comprises the following steps: S1, identifying and selecting vigna unguiculata disease-resistant varieties; s2, testing and comparing the treatment effects of different disease-resistant agents on the cowpeas, and selecting the disease-resistant agent with the best effect; s3, treating the seeds before sowing; s4, soil insect prevention and control; s5, soil-borne diseases and root diseases are prevented and controlled; s6, preventing and treating leaf diseases; s7, preventing and controlling pests on the ground; s8, using an immunopotentiator; and S9, field cleaning. According to the method, disease-resistant varieties are identified and screened in the early stage of sowing, a new variety of root rot resistant cowpeas is bred and screened out, diseases are reduced from the source, and the prevention and treatment effects of biopesticide on main diseases and insect pests in different growth periods of cowpeas are systematically studied; the green comprehensive prevention and control technology with biopesticide as the main part and chemical pesticide synergistic prevention and control as the core is specifically used, economic and ecological benefits are considered, and green and safe production of cowpeas is guaranteed.

The invention discloses an injection device for precisely applying pesticide to root diseases of Chinese pines, which comprises a placing frame, auxiliary discs are arranged at the upper end of the placing frame, a positioning pile is fixedly connected to the lower end of the placing frame, one end of the positioning pile is fixedly connected with a limiting plate, the number of the auxiliary discs is two, and the two auxiliary discs are symmetrically distributed; a movable bin is formed in the inner side of the auxiliary disc; an operation channel is provided for a soil breaking drill and a hole taking drill through auxiliary holes formed in an inclined panel, the auxiliary holes are used for breaking soil, conveying soil and drilling holes, meanwhile, the guiding and auxiliary stabilizing effects are achieved in the follow-up injection process, and by means of cooperation of an auxiliary plate and a clamping piece and cooperation of a positioning cylinder and a cross-shaped clamping block and a cross-shaped notch in a pressing injector, the pressing injector can be conveniently and rapidly pressed; the pressing injector is effectively prevented from deviating and misplacing in the using process, the injection accuracy is ensured, liquid medicine can accurately act on the disease portion of the root of the Chinese pine, and the treatment effect is improved.