24 results about "Root disease" patented technology

The invention discloses a method for visually detecting grape black root bacteria Ilyonectria sp. Based on RPA-CRISPR / Cas12a and application of the method for visually detecting the grape black root bacteria Ilyonectria sp. Based on the RPA-CRISPR / Cas12a. According to the method, an RPA specific amplification primer, a CrRNA probe and an ssDNA probe are adopted; according to the RPA specific amplification primer, an HIS gene is used as a specific recognition target, and specific amplification can be carried out on the grape black root pathogen Ilyonectria. The method for detecting the grape black root pathogenic bacteria has high specificity and sensitivity, is simple, convenient and rapid in operation flow, and is suitable for rapid screening and preliminary diagnosis of target pathogens in a field environment or a basic detection scene.

The invention belongs to the technical field of cortex acanthopanacis planting, and particularly relates to an under-forest cortex acanthopanacis water and fertilizer integrated planting method. The invention relates to an under-forest cortex acanthopanacis water and fertilizer integrated planting method, which comprises the following steps: S1, selecting a low-hill mountain land with the altitude of 200-400m and the gradient of lower than 15 degrees according to the tree age of 3-5 years and the canopy density of 0.25-0.35; s2, drip irrigation pipes are buried after soil preparation, then cortex acanthopanacis cutting slips are cut, clear water is poured thoroughly, and microbial water and fertilizer are dropped through the drip irrigation pipes in the subsequent planting process; wherein the microbial water fertilizer is formed by mixing bacillus safensis fermentation liquor and bacillus megatherium fermentation liquor. According to the method, in the under-forest cortex acanthopanacis integrated planting process, microbial water and fertilizer are subjected to drip irrigation, the prevention and treatment effect on cortex acanthopanacis root diseases can be effectively prevented and improved, and development of under-forest cortex acanthopanacis integrated planting is promoted.

The application discloses a bacillus velezensis A13 wettable powder and application thereof, and belongs to the technical field of biological pesticides. The application aims to solve the problems of limited prevention and treatment effect of existing seed treatment, high labor intensity of foliar spraying and short effective period. The wettable powder comprises a mother powder prepared from fermentation liquor and diatomite, and a dispersant, a wetting agent, a stabilizer and a protective agent, wherein the total addition amount of the dispersant and the wetting agent is 5%-14% of the mass of the mother powder, the addition amount of the protective agent is 0.5%-2% of the mass of the mother powder, and the addition amount of the stabilizer is 1%-3% of the mass of the mother powder. The application can be used for preventing and treating root diseases caused by panax quinquefolius rust rot fungus and leaf diseases caused by panax quinquefolius alternaria leaf spot fungus, and promoting plant growth.

The utility model discloses a multifunctional urban landscaping plant planting box, and particularly relates to the technical field of plant planting devices.The multifunctional urban landscaping plant planting box comprises a box body, a containing groove is formed in the upper end face of the box body, a planting plate is fixedly connected to the inner surface wall of the containing groove, and a water supplementing structure is arranged on the outer side of the planting plate; the water supplementing structure comprises a water storage tank arranged in the containing groove. The planting box has a water replenishing function by arranging the water replenishing structure, when the planting box is used, automatic on-demand water replenishing for green plants is achieved, water can be replenished in time sensitively and accurately according to the actual requirements of the plants, the root systems of the plants are soaked in the environment with the appropriate humidity all the time, and the planting box has the water replenishing function. The problem that growth is blocked due to water shortage is effectively avoided, the hidden danger of root diseases caused by excessive water is avoided, plants can thrive in a healthy and stable environment, it is guaranteed that the growth situation of the plants is good, and the long-term attractiveness and ecological stability of the whole greening landscape can be maintained.

The invention belongs to the technical field of crop disease and pest control, and particularly relates to a reagent for improving resistance of tobacco to phytophthora nicotianae and application. The reagent is a mixed solution of a copper sulfate solution and nano carbon sol. The reagent provided by the invention is green and environment-friendly, and has no hidden danger of environmental pollution; through external application, the resistance of tobacco to phytophthora nicotianae can be obviously enhanced, and the occurrence of tobacco root diseases is reduced. Compared with existing biological prevention and control methods and the like, the method for preventing and controlling phytophthora nicotianae adopts a solution root irrigation mode to prevent and control phytophthora nicotianae, has the potential advantages of being easy and convenient to operate, low in cost, rapid in effect taking, remarkable in effect, free of pollution, free of public nuisance, capable of achieving in-situ treatment and the like, and has the application prospect of application and popularization in the tobacco field.

The present invention provides a novel nucleic acid molecule encoding a polypeptide that can confer resistance to pathogens, especially the "Beet Necrosis Yellow Vein Virus" (BNYVV), in plants, especially in plants of the genus Beta expressing the polypeptide, and also provides preferred nucleic acid molecules encoding the RZ-3 gene of Beta maritima, as well as derivatives and homologues thereof. Further aspects of the invention include vectors, transgenic plant cells, transgenic plants, transgenic plants and methods of making them, and methods of identifying nucleic acid molecules that confer resistance.

The invention discloses a special organic fertilizer for tobacco planting, which relates to the technical field of organic fertilizers for tobacco, and is characterized in that the special organic fertilizer for tobacco planting comprises the following components in percentage by mass: 10-15% of oil cake, 30-35% of humic acid, 25% of straw, 10-15% of charcoal dust and compound bacteria, and the compound bacteria comprise bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens. The organic matter content of soil is increased, the activity of beneficial microorganisms is enhanced, flue-cured tobacco root system diseases are reduced, the soil air permeability is enhanced, and the tobacco growth trend is obvious; after being applied, the product is safe to personnel, soil environment and crop planting, the produced agricultural products are safe, and the numerical value can be stably reduced for a long time.

The invention relates to a planting method for interplanting stephania erecta in a rubber forest, and belongs to the technical field of agricultural planting. The invention provides a planting method for interplanting stephania erecta in a rubber forest. The planting method comprises the steps of forest land selection, soil improvement, stephania erecta planting and cultivation management. According to the method, the stephania erecta is interplanted in the rubber forest, the blank forest land in the rubber forest is fully utilized, meanwhile, the problem that environmental lighting needs to be set deliberately for stephania erecta planting is solved, the stephania erecta and the rubber trees are harmonious and symbiotic through the planting method, and the yield of the stephania erecta is increased. The rattan trimmed from the stephania erecta is used as green manure to be applied to the rubber tree, so that the rubber yield of the stephania erecta can be increased, meanwhile, the weight of stephania erecta tuberous roots can be increased by applying decomposed leaves of the rubber tree as fertilizer to the stephania erecta, the number of root-knot nematodes in forest land can be remarkably reduced through planting of the stephania erecta, and root diseases of the rubber tree are reduced.

The invention discloses an injection device for precisely applying pesticide to root diseases of Chinese pines, which comprises a placing frame, auxiliary discs are arranged at the upper end of the placing frame, a positioning pile is fixedly connected to the lower end of the placing frame, one end of the positioning pile is fixedly connected with a limiting plate, the number of the auxiliary discs is two, and the two auxiliary discs are symmetrically distributed; a movable bin is formed in the inner side of the auxiliary disc; an operation channel is provided for a soil breaking drill and a hole taking drill through auxiliary holes formed in an inclined panel, the auxiliary holes are used for breaking soil, conveying soil and drilling holes, meanwhile, the guiding and auxiliary stabilizing effects are achieved in the follow-up injection process, and by means of cooperation of an auxiliary plate and a clamping piece and cooperation of a positioning cylinder and a cross-shaped clamping block and a cross-shaped notch in a pressing injector, the pressing injector can be conveniently and rapidly pressed; the pressing injector is effectively prevented from deviating and misplacing in the using process, the injection accuracy is ensured, liquid medicine can accurately act on the disease portion of the root of the Chinese pine, and the treatment effect is improved.

The invention provides Rhodococcus qingsheng and application thereof in relieving continuous cropping obstacles of crops, and belongs to the technical field of microorganisms. The Rhodococcus qingsheng JA11 is separated and screened from the nature for the first time, phenolic acid autotoxic substances generated by ginseng continuous cropping obstacles can be efficiently degraded, the degradation rate of benzoic acid can reach 91.51%, various phenolic acids such as vanillic acid and cinnamic acid can be effectively degraded, and the degradation spectrum is wide; when the Rhodococcus qingsheng JA11 is applied under field conditions, the content of benzoic acid in soil for continuous cropping of ginseng can be effectively reduced, meanwhile, the root diseases of ginseng can be controlled to a certain extent, the growth indexes such as the survival rate and the root length of ginseng are remarkably improved, and a certain yield increasing effect is achieved.

The utility model belongs to the field of agricultural mulching films, and provides a polylactic acid degradable agricultural mulching film with an antibacterial function mainly aiming at the problem of threat of soil pathogenic microorganisms to crop health at present, which comprises a polylactic acid support layer, the mulching film is characterized in that the mulching film further comprises an antibacterial functional layer stacked on the polylactic acid supporting layer, the antibacterial functional layer comprises a structural body formed by compounding cellulose nanofibers and chitosan, the diameter of the cellulose nanofibers is 10-60 nm, and the length-diameter ratio of the cellulose nanofibers is 50-200; the cellulose nanofibers form a continuous three-dimensional network in the antibacterial functional layer, and a uniform and compact chitosan layer is tightly attached in the three-dimensional network. Due to the existence of the antibacterial functional layer with the specific composite layer structure, the mulching film can effectively restrain growth and reproduction of crop pathogenic bacteria, root diseases are reduced, and the mulching film can be used as an agricultural mulching film in agricultural production.

The invention provides streptomyces albidoflavus and application thereof, and relates to the technical field of microorganisms. The streptomyces alboflavus disclosed by the invention is named as Streptomyces alboflavus LY7, and the preservation number of the streptomyces alboflavus LY7 is CGMCC (China General Microbiological Culture Collection Center) No.37530. The strain has an inhibition effect on various phytopathogens, and has great potential in agricultural applications such as plant disease control. Related application and products of the biocontrol bacterium streptomyces albidoflavus, such as multifunctional bacterial manure taking needle mushroom bran as a matrix, are expanded, and the streptomyces albidoflavus has obvious bacteriostatic activity on main soil-borne disease pathogenic bacteria of ginseng, and can effectively prevent and treat root diseases of ginseng; the ginseng growth can be obviously promoted; the ginseng yield is increased. In addition, the microbial fertilizer can also effectively regulate the physicochemical properties of soil in the ginseng field, improve the enzyme activity of the soil and promote the healthy development of the ginseng industry.

The application provides a strain of rhodococcus qingshengii and application thereof in relieving continuous cropping obstacles of crops, and belongs to the technical field of microorganisms.The rhodococcus qingshengii JA11 is first isolated and screened from nature, can efficiently degrade phenolic acid autotoxic substances generated by continuous cropping of ginseng, the degradation rate of benzoic acid can reach 91.51%, can effectively degrade various phenolic acids such as vanillic acid and cinnamic acid, and has a wide degradation spectrum; and under field conditions, application of the rhodococcus qingshengii JA11 can effectively reduce the content of benzoic acid in the soil for continuous cropping of ginseng, can control the occurrence of root diseases of ginseng to a certain extent, significantly improves the survival rate, root length and other growth indexes of ginseng, and has a certain yield-increasing effect.

A new and distinct cultivar of Lavandula x intermedia plant named ‘SENS-M1’ is disclosed, characterized by white flowers held on stiff, thick, very densely flowering stems. Plants are very compact and strongly upright. Flower stems are very thick and branched, resulting in rebloom for a longer blooming season. The fragrance is strong and more floral than herbal / pungent. Tolerance for leafspot and root disease has been observed. The new cultivar is a Lavandula x intermedia, normally used for outdoor ornamental purposes.

This invention relates to a microbial composition for controlling root-knot nematode disease in traditional Chinese medicinal materials and its application, belonging to the field of biotechnology. The composition comprises the following components: *Beauveria bassiana* (CGMCC No. 21047), lecithin, xanthan gum, wood vinegar, soybean peptides, potassium humate, and trace elements. Through the synergistic effect of specific functional microbial communities, the composition significantly promotes the growth of the underground parts of traditional Chinese medicinal materials, increases root biomass and root vigor, and enhances the plant's efficiency in nutrient absorption and utilization, particularly nitrogen utilization. Simultaneously, this preparation effectively controls root-knot nematode disease, significantly reducing the number of root-knot nematode infestation sources in the soil and the severity of root disease, achieving a high natural nematode reduction rate. Furthermore, this preparation activates the plant's own defense system, induces the synthesis of disease-resistant substances, and significantly enhances the activity of various defense enzymes. It also significantly improves the soil microecological environment, enhances the diversity and richness of the soil bacterial community, and is suitable for various crop planting systems.

The invention discloses a traditional Chinese medicine compound for treating diabetic retinopathy and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine compounds. The traditional Chinese medicine compound is prepared from the following traditional Chinese medicine raw materials: radix astragali, radix salviae miltiorrhizae, radix rehmanniae recen, fructus lycii, radix notoginseng, radix puerariae, radix ophiopogonis, rhizoma chuanxiong, radix angelicae sinensis and pollen typhae. The traditional Chinese medicine has the effects of tonifying qi, nourishing yin and dredging collaterals and can effectively treat diabetic retinopathy. According to multi-target cooperation, oxidative stress, inflammatory response and retina microcirculation are improved at the same time, and the effect is more comprehensive compared with that of single anti-VEGF. The composition is safe to take orally, has no vitreous injection risk and can be used for a long time. The method is controllable in quality: establishing a characteristic fingerprint spectrum and quantizing index components, and accords with GMP (good manufacturing practice) batch production. The cost is low, raw materials are easy to obtain, and basic-level chronic disease management requirements are met.

This invention discloses a device for controlling root diseases and pests in fruit trees, comprising: a housing; a rotating cleaning structure, comprising: a first plate having a plurality of first through holes; a second plate disposed below the first plate by a first elastic element, with a first gap between the second plate and the first plate, in which an elastic ball is disposed, and a second through hole on the second plate; a stirring structure comprising a motor, a rotating shaft, and stirring blades, the rotating shaft passing through the first plate and the second plate, the rotating shaft being fixedly connected to the first plate, the motor being fixedly connected to the upper end of the rotating shaft, the motor being fixed above the housing by a fixing bracket, the stirring blades being disposed on the rotating shaft and located below the second plate; and an electric shock assembly comprising a power supply and an electric shock plate, the power supply being connected to the first plate and the electric shock plate. This invention can clean the soil attached to the roots of fruit trees, so as to electrocute the root-attached pests and diseases, thereby improving the control efficiency of root diseases and pests.

The utility model belongs to the field of agricultural mulching films, and provides a PBAT (poly (butyleneadipate-co-terephthalate)) degradable agricultural mulching film with an antibacterial function mainly aiming at the problem of threat of soil pathogenic microorganisms to crop health at present. The mulching film comprises a PBAT supporting layer and is characterized in that the mulching film further comprises an antibacterial functional layer stacked on the PBAT supporting layer, the antibacterial functional layer comprises a structural body formed by compounding cellulose nanofibers and chitosan, the diameter of the cellulose nanofibers is 10-60 nm, the length-diameter ratio of the cellulose nanofibers is 50-200, and the length-diameter ratio of the chitosan is 50-200. The cellulose nanofibers form a continuous three-dimensional network in the antibacterial functional layer, and a uniform and compact chitosan layer is tightly attached in the three-dimensional network. Due to the existence of the antibacterial functional layer with the specific composite layer structure, the mulching film has an excellent antibacterial effect, growth and reproduction of crop pathogenic bacteria can be effectively inhibited, root diseases are reduced, and therefore the mulching film has a good application prospect in the agricultural field.

The invention discloses an optimized preparation process of a charcoal fungicide for preventing and treating delonix regia root rot, and belongs to the field of microbial fermentation optimization. The process comprises the following steps: firstly, carrying out bacterial liquid culture (culture at 32 DEG C and 200rpm) on Pseudomonas aeruginosa and Bacillus licheniformis through an LB liquid culture medium, then carrying out liquid culture medium optimized fermentation on 3% of bacterial liquid (culture at 32 DEG C and 220rpm for 24 hours under the conditions of 3% of sucrose, 0.5% of ammonium sulfate, 1% of beef extract and 2% of potassium chloride) so as to obtain optimized fermentation liquor, and carrying out fermentation on the optimized fermentation liquor so as to obtain the Pseudomonas aeruginosa and Bacillus licheniformis. And mixing the optimized fermentation liquor and charcoal according to the mass ratio of 5: 1, and standing at normal temperature for 24 hours to obtain the fungicide. In a greenhouse experiment, the control effect on the delonix regia root rot reaches 83.53%. The biochar fungicide has the characteristics of high efficiency, environment friendliness, no pollution and the like, is suitable for preventing and treating root diseases of delonix regia and other garden plants, and has a wide application prospect.

The invention belongs to the technical field of agricultural ecological cultivation and plant protection, and discloses a method for reducing fresh pea root rot in a flue-cured tobacco-fresh pea rotation mode based on synergism of rotation and a microbial agent. The corn is used for replacing the third-year Dahun flue-cured tobacco; in the third year, when the spring crop corn is sown, a Purpureocillium lilacinum and Trichoderma asperellum complex microbial inoculant is applied in a matched manner; after garlic bulbs are harvested, healthy straw and root system residues are smashed and returned to the field, soil humidity is kept, and biological fumigation is carried out; after the corn season, straw is smashed, deeply ploughed and returned to the field. The three measures have a synergistic effect to jointly strengthen the inhibition and removal functions on the pathogenic bacterium dormant bodies of the pea root rot in the soil, and the micro-ecological health of the soil is remarkably improved, so that strong root disease system resistance is provided for later fresh peas. On the premise that an original crop rotation system framework is not damaged, green and efficient ecological prevention and control of soil-borne diseases are achieved.

The invention discloses a combined spicebush leaf thiazole extract and a preparation method and application thereof, and the preparation method of the combined spicebush leaf thiazole extract comprises the following steps: soaking and extracting dry combined spicebush leaf powder by using thiazole, repeatedly extracting for 2-5 times, merging extracting solutions, and carrying out centrifugal concentration to obtain the combined spicebush leaf thiazole extract. The combined spicebush leaf thiazole extract provided by the invention can obviously reduce the odor of fosthiazate, is easier to accept in the use process, can effectively prolong the lasting period of a medicament in the process of preventing and treating root-knot nematode damage of crops, has an obvious inhibition effect on root diseases of the crops, and can be used for preventing and treating the root-knot nematode damage of the crops. The resistance of crops to plant diseases and insect pests is improved.

The invention relates to the technical field of agricultural cultivation, in particular to a fertilizing method for increasing the yield of jackfruits. According to the fertilizing method, the soybean meal organic fertilizer, the animal source organic fertilizer and the calcium magnesium phosphate fertilizer are applied according to a specific ratio at a specific time, and the fertilizing ditches are backfilled in combination with branches, leaves and weeds, so that the dependence on chemical fertilizers can be effectively reduced, the diversity of soil microorganisms is increased in multiple ways, and the biological fertility of soil is remarkably improved; therefore, the growth of the jackfruits is effectively promoted, and the yield of the jackfruits is remarkably increased. In addition, by means of local root cutting and adjustment of topdressing and irrigation modes, root diseases can be reduced, sandy soil fertilizer and water loss is avoided, and labor cost is saved. The composition can fix carbon and lose weight, and plays an important role in promoting quality and efficiency improvement of the jackfruit industry and maintaining a healthy agricultural ecological environment.

The application discloses a slow-release fumigation treatment method for potted flower root diseases, and comprises the following steps: S1, preparing a slow-release fumigation agent; S2, determining an application point; S3, drilling a hole and applying the fumigation agent; and S4, covering soil and watering. The application relates to the technical field of horticultural plant protection, in particular to a slow-release fumigation treatment method for potted flower root diseases, which aims to overcome the shortcomings of the prior art and provide a slow-release fumigation treatment method for potted flower root diseases. The method constructs a special slow-release fumigation agent, applies the fumigation agent to a specific position of the pot soil, triggers the slow and continuous release of the fumigation active ingredient by using soil humidity and microbial activity, forms a long-term and stable low-concentration fumigation environment in the root zone, and thus efficiently prevents and treats diseases and is safe to plants.