208 results about "Bacillus methylotrophicus" patented technology

Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

The invention discloses a plant bacteriostasis method adopting the bacillus methylotrophicus strain NKG-1. Pesticide is prepared from bacillus methylotrophicus strain NKG-1 bacteria liquid, and the prepared pesticide methylotrophicus agentia is applied in the growing period of plants to inhibit germs on the plants. By the adoption of the plant bacteriostasis method adopting the strain, various pathogenic fungi and bacteria can be inhibited, a good control effect is realized, and therefore application prospects are broad.

The invention discloses a novel bacillus methylotrophicus, which has the preservation register number of CGMCC No.6793, wherein the strain is separated from tobacco rhizosphere soil and is obtained by separating and purifying and screening strains. The invention further provides a fermentation method of the bacillus methylotrophicus, a fermentation product prepared by the method, as well as a biological agent based on the fermentation product and a preparation method thereof; the invention further provides a use of the biological agent in controlling a variety of plant diseases. According to the invention, the biological agent is good in environmental compatibility so that the number of effective living spores is more than 5,000,000,000 / g, has high biological activity, and in particular has good control effect on tobacco bacterial wilt, wherein the control effect on tobacco bacterial wilt, soft rot of cabbage, rice bacterial leaf blight, rice bacterial stripe and cotton angular leaf spot is up to 80% or more.

The invention discloses bacillus methylotrophicus F7 and an application thereof. The invention aims at providing a strain which has a plurality of beneficial properties, namely producing siderophore, cellulase and protease, producing auxin, producing ammonia and the like; the strain not only can be used for effectively inhibiting various pathogenic bacteria such as ralstonia solanacearum, fusarium oxysporum f.sp.cubense, colletotrichum gloeosporioides, fusarium oxysporum f.sp cucumerinum but also can promote plant growth. In accordance with the technical scheme, the bacillus methylotrophicus F7 is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 1, 2016, with preservation number of CGMCC No.12124; in addition, five surfactin homologs, six fengycin homologs and three iturin homologs, which have an antibacterial effect, are extracted from fermentation liquid of the bacillus methylotrophicus F7; and the bacillus methylotrophicus F7 belongs to the field of agricultural biotechnology.

The invention discloses bacillus methylotrophicus wettable powder which comprises 100 parts by mass of bacillus methylotrophicus active components, 4-10 parts by mass of a wetting agent and 4-10 parts by mass of a dispersing agent. A method for preparing the bacillus methylotrophicus wettable powder comprises the following steps: weighing the bacillus methylotrophicus active ingredients, the wetting agent and the dispersing agent according to a ratio, and uniformly mixing; and milling through a jet mill, thereby obtaining the bacillus methylotrophicus wettable powder. The wettable powder is stable in quality and convenient to preserve and use for a long time, has good effects of preventing and treating cucumber gray mold and apple rot, solves the problems such as environmental toxic and side effects and pesticide residues brought by chemical agents, has the characteristics of high efficiency, no toxicity, safety and no residue, is a safe, effective, low-residue and environment-friendly microbial pesticide and has wide application prospects in the field of microbial control.

The invention provides bacillus methylotrophicus SZ-3 and SZ-81, with collection numbers of CGMCC No.8275 and CGMCC No.8276 respectively. The bacillus methylotrophicus SZ-3 has obvious antagonistic effects on pathogenic bacteria causing root rot, black spot and sclerotinia sclerotiorum of ginseng. The bacillus methylotrophicus SZ-81 has obvious antagonistic effects on pathogenic bacteria causing epidemic diseases, root rust rot and black spot of panax ginseng. The invention also provides an application of the bacillus methylotrophicus SZ-3 and SZ-81 to control of fungal diseases of plants or an application to preparation of microbial preparations for controlling fungal diseases of plants.

The invention discloses a complex microbial agent for composting fermentation and application thereof. The complex microbial agent is a solid inoculant which comprises phanerochaete chrysosporium, streptomyces griseorubens, bacillus subtilis, bacillus methylotrophicus and bacillus amyloliquefaciens. The complex microbial agent is a complex microbial system comprising various microorganisms which have complementary and synergistic functions; the microbial system integrates quick fermentation heat production, biological deodorization and compost material decomposition and degradation and has the effects of a biological fertilizer; the livestock manure compost can be quickly heated to kill off harmful organisms at high temperature; the unpleasant smell is obviously reduced to solve the problem of environment pollution caused by poultry excrement; the microorganisms can be quickly propagated in the poultry excrement fermentation process; the organic matters such as lignocellulose in the excrement can be quickly and effectively decomposed so as to increase the decomposition speed of the compost material and improve the quality of the compost product; the complex microbial agent can be used for producing the biological organic fertilizer so as to reduce the agricultural production cost.

The invention discloses a strain bacillus methylotrophicus 4-L-16 for prevention and control of banana vascular wilt. The strain has a preservation name of bacillus methylotrophicus 4-L-16, and is classified and named as Bacillus methylotrophicus 4-L-16. The preservation organization is China Center for Type Culture Collection, the preservation date is June 26, 2012, and the preservation number is CCTCC NO:M2012248. The strain has a strong antagonistic effect on No.1 and No.4 physiological races of banana vascular wilt, and other banana common disease categories. The invention also discloses application of the strain in preparation of drugs with a banana vascular wilt prevention and control function, as well as a microbiological preparation containing the strain. The microbiological preparation not only has a better antagonistic effect on a variety of banana pathogenic bacteria, but also has a facilitating effect on plant growth.

The invention discloses a bacillus and application thereof, and belongs to the field of biological control. A bacillus strain is bacillus methylotrophicus C1, and a preservation number of the bacillus strain is GDMCC No.60046. The application includes that the bacillus can be used as a biological control strain to be applied to antagonistic crop pathogenic bacteria; the bacillus can be used as a lipopeptide and / or protein fermentation strain for inhibiting crop pathogenic bacteria; the bacillus can be used as a growth promotion strain to be applied to promoting growth of crops; the bacillus can be used as a fermentation strain for auxin. The bacillus and the application have the advantages that the strain has broad-spectrum antibacterial activity; lipopeptide substances with effects of inhibiting fusarium graminearum cannot be generated, but proteins with effects of inhibiting the fusarium graminearum can be generated; effects of promoting growth of corn can be realized by the strain C1, the root lengths and the stalk lengths of the corn of experiment groups are 3.15 and 3.05 times the root lengths and the stalk lengths of contrast groups respectively as compared with contrast, the root, stalk and seed weights of the corn of the experiment groups are 5.02, 4.33 and 1.4 times the root, stalk and seed weights of the contrast groups, and only little auxin can be generated by the strain C1.

The invention relates to a Bacillus methylotrophicus Sanju-04 and its application. The above strain is preserved at China Center for Type Culture Collection (CCTCC) in Wuhan University, has a preservation date of May, 16, 2012, and has a preservation number of CCTCC No:M2012169. The Bacillus methylotrophicus Sanju-04 has very good capsicum bacterial wilt and orange anthracnose control efficacies, and has the advantages of stability, no toxic chemical substance residual, no pollution to agricultural products or the environment, high cost performance, and application convenience. The Bacillus methylotrophicus Sanju-04 plays an important role in the control of the bacterial wilt and the anthracnose of plants, and has a wide application prospect.

The invention relates to a biocontrol microorganism of wide spectrum antagonistic plant pathogenic fungi and application thereof, belonging to the technical field of microbial pesticides. The biocontrol microorganism is a Bacillus methylotrophicus Mo-Bm strain preserved in a CGMCC (China General Microbiological Culture Collection Center) on 12th October 2010 with a preservation number of CGMCC No.4207. A 16S rRNA gene sequence (with a sequence number of HQ325853 in a GeneBank database) of the Mo-Bm strain is a main characteristic basis for identifying the strain; and the Mo-Bm strain can resist 0.5-2 percent (V / V) carbinol, and is a secondary characteristic basis. The microorganism and metabolins thereof are prepared into a Mo-Bm wettable powder biocontrol anti-bacteria agent together with pesticide conventional absorption carriers and aids. The microorganism has a control effect of above 70 percent on tobacco black shank, cruciferclubroot and pseudo-ginseng root rot. The biocontrol microorganism has the characteristics of low cost, high control effect, no residue and safety to people and livestock, and is an efficient biological pesticide for controlling fungus diseases during organic agriculture planting.

The invention provides bacillus methylotrophicus UTM401 and applications thereof. The biological collection number of the bacillus methylotrophicus UTM401 is CGMCC No.5927. The bio-fertilizer can be prepared by inoculating the fermentation seed liquid of the strain UTM401 into an organic waste bank and fermenting. By adopting the strain UTM401 to prepare the bio-fertilizer, the cost is low, the quality is stable, and the fermentation period can be shortened; moreover, the bacteria are increased quickly again in solid material, and a stable bacterial colony system is formed with a fermentation material carrier; and high-density colonization of the plant rhizosphere can be realized, and the effects of suppressing phytopathogen and harmful microorganisms of rhizosphere, promoting the plant growth and improving the crop yield are realized. The strain UTM401 and the bio-fertilizer prepared from the fermentation organic waste thereof are green and environment-friendly and pollution-free, and have good performance and relatively high economic and social benefits.

The invention relates to a wettable powder of bacillus methylotrophicus. The wettable powder is composed of raw powder of bacillus methylotrophicus that is prepared with a specific method, light calcium carbonate, sodium dodecyl benzene sulfonate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate and 2-3 parts by weight of a dispersant methylene dimethyl naphthalene sodium sulfonate. Furthermore, the invention relates to a use of the wettable powder in controlling citrus diseases, in particular citrus canker.

Disclosed are methods of producing carotenoid compounds in a methylotrophic bacterial host cell. Such a host cell may be an unmodified Methylobacterium, spontaneous mutant, or transformed cell, any of which exhibit favorable properties, such as overproduction of carotenoid compounds, increased carbon flux, improved growth, or the production of additional nutrients, such as protein, vitamins, antioxidants, or fatty acids. Also disclosed are feed compositions for use in aquaculture, or as animal feed, or as human nutritional supplements containing processed or unprocessed biomass from such cells, as are methods of preparation of the feed compositions.

The invention provides bacillus methylotrophicus with a collection number of CCTCC M2015297. Bacillus methylotrophicus is named bacillus methylotrophicus XK-4. The invention further provides a screening method of bacillus methylotrophicus XK-4, an application of bacillus methylotrophicus XK-4 to inhibition of sclerotinia sclerotiorum, rhizoctonia solani, fusarium solani and fusarium oxysporum and a bacillus methylotrophicus XK-4 agent. Bacillus methylotrophicus XK-4 is a rape rhizosphere microorganism, is safe to human, livestock and crops, is environment-friendly, has a wider antibacterial spectrum, has better inhibitory effects on mycelial growth of common pathogenic bacteria, such as sclerotinia sclerotiorum, rhizoctonia solani, fusarium solani and fusarium oxysporum, has obvious inhibitory effects on mycelial growth of sclerotinia sclerotiorum and causes mycelia deformation. The further prepared agent has obvious control effects on sclerotinia rot of colza, is short in production cycle and has a very extensive application prospect in biological control of plant diseases.

The present invention discloses a strain of Bacillus methylotrophicus VJ4-1, and a method for producing natural vanillin by ferulic acid biotransformation with the Bacillus methylotrophicus VJ4-1, wherein a name of the strain is Bacillus methylotrophicus VJ4-1, the strain is preserved in the China center for type culture collection (CCTCC) in Wuhan University on January 16, 2012, and a preservation number is CCTCC NO:M2012004. The method of the present invention has advantages of high yield, simple process, mild reaction conditions, less environmental pollution, high product concentration, simple extraction step, clean production, environmentally friendly products, safety, reliability, wide main raw material source, low cost, and natural property, and has an industrial production prospect.

The invention discloses an application of Bacillus methylotrophicus ZBL-1 in control of cotton verticillium wilt. The Bacillus methylotrophicus ZBL-1 with CGMCC No.11636 and higher inhibition effect on verticillium dahlia is obtained through separation, screening, breeding and domestication of soil samples collected in the cotton fields with severe diseases in Shihezi city and is obviously different from common Bacillus methylotrophicus strains, and meanwhile, the control effect of the screened strain ZBL-1 on the cotton verticillium wilt in indoor pot culture experiments is 73.0%; the control effect of the screened strain ZBL-1 in field plot experiments is 43.1%, and whether in development of a new biopesticide or biological control agent, the Bacillus methylotrophicus ZBL-1 as a biological control material for the cotton verticillium wilt has very good application prospect and has realistic significance and functions.

The invention provides an antibacterial agent special for potatoes. The antibacterial agent comprises bacillus subtilis and paecilomyces lilacinus; the weight ratio of the bacillus subtilis to the paecilomyces lilacinus is 6-9 to 1; the bacillus subtilis at least comprises three of bacillus laterosporus, bacillus methylotrophicus, bacillus licheniformis, bacillus megatherium, bacillus subtilis, paenibacillus mucilaginosus and bacillus amyloliquefaciens. The antibacterial agent special for the potatoes, which is prepared by the invention, can reasonably utilize interaction among microorganisms,has high adaptability, high stability and high pathogen antagonism, can efficiently antagonize pathogenic bacteria of potato early blight and sore coffee diseases, can stimulate plant growth and canimprove crop quality so as to fulfill the yield-increasing and high-yield aims.

The invention provides three biocontrol bacilli namely bacillus methylotrophicus AR3, bacillus subtilis AR4 and bacillus amyloliquefaciens AR10 and application thereof to preparation of a crop ralstonia solanacearum resistant bacteria agent. According to the invention, three biocontrol bacilli namely bacillus methylotrophicus AR3, bacillus subtilis AR4 and bacillus amyloliquefaciens AR10 are obtained through soil sample taking, screening, analysis and cultivation in a healthy tobacco field in the Xuan'en county, results of plenty of field experiments prove that when being used separately or used in a mixed manner, the three biocontrol bacilli have the good effect of preventing and controlling crop ralstonia solanacearum, are highly targeted and low in pollution, and can be stored for a long term and conveniently used in the field after being prepared into the bacteria agent, so that the achievement of the bacilli in the field of biocontrol bacterium research is enriched and guiding significance on later related research work is realized.

The invention discloses an enzymolysis-fermentation coupled aquatic protein active peptide preparation method. The method mainly includes the following steps that fresh aquatic protein is minced; the minced aquatic protein is added to an enzymolysis tank, nutrients and water are added, and the mixture is mixed to be uniform to serve as a fermentation culture medium; the enzymolysis tank is inoculated with high-temperature-resistant facultative anaerobe Bacillus methylotrophicus HL-4 capable of generating protease, and meanwhile enzymolysis and fermentation are conducted; fermentation liquor is subjected to filtration, ultra-filtration and nano-filtration in sequence, then concentrated liquor is obtained, and the finished active peptide is obtained after freeze drying of the concentrated liquor. According to the technology, the enzymolysis process and the fermentation process are completed in the enzymolysis tank at the same time, and equipment and operation are simplified; acting conditions are mild, the biological activity of functional components in the prepared functional peptide and the aquatic protein is better preserved, and good application prospects are achieved.

The invention discloses a Bacillus methylotrophicus strain VJ4-1 of which the preservation number is CCTCC No:M 2012004. The invention provides a tobacco spine date seed extract prepared by using microbe Bacillus methylotrophicus and conducting biotransformation and an application of the spine date seed extract in tobacco. According to the invention, by fermenting the spine date seeds with the microbial strain, a natural flavor, i.e. the tobacco spine date seed extract can be produced; the method has the advantages of simple process, wide sources of main raw materials, and environmentally friendly and harmless production process, and has an industrial production prospect; the obtained natural flavor can be directly used for adding fragrance for cigarettes, is in harmony with the smoke, adds the sweet and fragrance of cream and fragrance of flower, makes the fragrance soft and comfortable, and improves the tasting, and can be mixed with other tobacco flavors.

The present invention relates to a method of producing a recombinant peptide, a recombinant protein, or a product from metabolic engineering using a genetically modified methylotrophic bacterium, and more particularly to Methylobacterium extorquens ATCC 55366. The method comprises introducing am expression vector into the methylotrophic bacterium, the expression vector comprising a polynucleotide sequence, coding for a peptide or protein, or allowing for production of a product from metabolic engineering under the control of a regulated promoter. the method also comprises growing the genectically modified methylotrophic bacterium in a minimal salts medium lacking organic sugars and containing methanol. A metal ion may be used for regulating the expression of the polynucleotide sequence by the promoter.

Provided is a method for expressing an introduced gene or genes in a C1 metabolizing microorganism host wherein the gene(s) are integrated into the tig region of the chromosome. This method provides high level expression in a stable manner in which growth rate of the host strain is not highly affected and a selection marker is not required. The use of this method for expressing carotenoid biosynthetic genes and resulting production of canthaxanthin is also described.

The invention discloses bacillus methylotrophicus B18 as well as a liquid preparation prepared from the bacillus methylotrophicus B18 and application of the bacillus methylotrophicus B18. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 18, 2017 and has a preservation number of CGMCC No. 14641. The liquid preparation is prepared by taking thebacillus methylotrophicus B18 as the strain and carrying out seed cultivation and fermentation cultivation. The biocontrol bacterium-bacillus methylotrophicus B18 strain has double functions of disease prevention and growth promotion, has the characteristics of biological control and bio-fertilizer, and has remarkable effects on the control of damping-off of eucommia ulmoides and the promotion ofgrowth of the eucommia ulmoides; the bacillus methylotrophicus B18 not only can avoid the serious consequences of environmental pollution caused by chemical drugs, but also has the characteristics ofsustainability and low cost.

The invention discloses Bacillus methylotrophicus and an application of the Bacillus methylotrophicus on the aspect of preventing and controlling fusarium graminearum. The preservation number of the Bacillus methylotrophicus disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 9500, and the nucleotide sequence of the Bacillus methylotrophicus is shown as SEQ ID No. 1. The bacterial strain is used for the application of biological prevention and control in plant diseases caused by the fusarium, wheat and corns serve as biological prevention and control materials for crop diseases; no matter new biological pesticide or biological prevention and control fungicide is developed, the bacterial strain has an excellent application prospect.

The invention discloses a method for preparing liquid organic fertilizer through fermentation of aquatic product waste. The method comprises main steps as follows: fresh aquatic product waste minced; the minced aquatic product waste is added to an enzymolysis tank and nutrients and water are added and mixed evenly to be used as a fermentation medium; the enzymolysis tank is inoculated with high-temperature-resistant facultative anaerobe methyl nutritional type Bacillus methylotrophicus HL-4 capable of producing protease for simultaneous enzymolysis and fermentation; a fermented broth is filtered for precipitation removal, and thus the liquid organic fertilizer can be prepared. Enzymolysis and fermentation processes can be finished in the enzymolysis tank simultaneously, equipment and operation are optimized, the production cost of the liquid organic fertilizer is significantly reduced, and the method has good application prospect.

The invention relates to the field of biopesticides and microbial fertilizers and particularly discloses bacillus methylotrophicus wettable powder containing a spore germination agent and a preparation method thereof. The wettable powder is prepared from the following raw materials in parts by weight: 40-70 parts of bacillus methylotrophicus, 20-30 parts of talcum powder serving as a padding, 1-4 parts of sodium dodecyl benzene sulfonate serving as a wetting agent, 0.2-1 part of tea saponin serving as a dispersing agent, 0.1-0.5 part of methionine and 0.1-0.5 part of 2,6-dipicolinic acid calcium salt serving as a spore germinator, 0.5-2 parts of ascorbic acid and 0.2-1 part of beta-dextrin serving as an ultraviolet protector, and 1-4 parts of maltose and 0.2-1 part of mannitol serving as a dryness protector. After the bacillus methylotrophicus wettable powder prepared by the preparation method is applied to a land for growing field crops, the spore germination speed is rapid, and the germination rate is high; and the bacillus methylotrophicus wettable powder is capable obviously improving the fertilizer efficiency and plant disease control effect, reducing the spore dosage and lowering the cost.

The invention discloses a compound fungicide FX for degrading straws. The compound fungicide FX comprises a Bacillus subtilis strain NJX501, a Bacillus subtilis strain NJX502, a Bacillus methylotrophicus strain NJXy and an Aspergillus fumigatus strain NJZ501. The compound fungicide is prepared by mixing the bacterium liquid of NJX501 with a bacterium OD value of 0.5-1, the bacterium fluid of NJX502 with a bacterium OD value of 0.5-1, the bacterium liquid of NJXy with a bacterium OD value of 0.5-1 and NJZ501 spore suspension with spore number of not less than 104 / ml in equivalent volume. The compound fungicide FX can be used for promoting the composting and decomposition of rice straws under high temperature conditions.

The invention discloses a green alga glycosyl microcapsule biological agent and its preparation method and application. Four strains Geobacillus stearothermophilus, Bacillus vallismortis, Bacillus methylotrophicus and Bacillus siamensis are mixed and compounded in proportion; and by a microencapsulation technology which is most effective and practical for protection of strain activity, the active strains are wrapped in a capsule wall substance with Enteromorpha prolifera polysaccharide being the main component. Thus, the green alga glycosyl microcapsule biological agent is prepared. In addition, the invention specifically provides a preparation method of the green alga glycosyl microcapsule biological agent. The green alga glycosyl microcapsule biological agent provided by the invention is pollution-free and can achieve the efficacy of cooperative usage of a fertilizer and a pesticide. The technology is simple and costs are low. The environment is protected. The product has high disease resistance and stress resistance. Survival rate is enhanced. The product is easy for industrial production.

The invention relates to bacillus methylotrophicus, bactericide and a preparation method and application of the bactericide.The bacillus methylotrophicus is bacillus methylotrophicus JQ-018 and is preserved in China General Microbiological Culture Collection Center on 7th December, 2015, and the preservation register number is CGMCC No:11838.The bactericide can be prepared through seed activation, seed culture and fermentation culture.The invention further provides the preparation method of the bactericide of the bacillus methylotrophicus and the application to preparation of an agent for preventing and controlling potato early blight.The bactericide has high bioactivity and good environment compatibility, is free of poisons to people and livestock and convenient to use, and can adjust plant microorganism ecological florae.