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Bacillus methylotrophicus B18 as well as liquid preparation prepared from bacillus methylotrophicus B18 and application of bacillus methylotrophicus B18

A methylotrophic, bacillus technology, applied in the field of microorganisms, can solve the problems of large environmental pollution, unsafe human and livestock, etc., and achieves the effect of broad market prospects and low cost

Active Publication Date: 2018-03-30
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are very few studies on the disease, and the control is usually carried out by crop rotation, scientific management, formalin, zinc zinc, etc. Chemical pesticides cause great environmental pollution and are unsafe for humans and animals.

Method used

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  • Bacillus methylotrophicus B18 as well as liquid preparation prepared from bacillus methylotrophicus B18 and application of bacillus methylotrophicus B18
  • Bacillus methylotrophicus B18 as well as liquid preparation prepared from bacillus methylotrophicus B18 and application of bacillus methylotrophicus B18
  • Bacillus methylotrophicus B18 as well as liquid preparation prepared from bacillus methylotrophicus B18 and application of bacillus methylotrophicus B18

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Methylotrophic Bacillus B18

[0029] 1.1 Isolation of methylotrophic Bacillus B18

[0030] Healthy Phellodendron bark was collected from the area where Phellodendron rotten skin disease occurred in Yangtianwo Pharmacy, Dayi, Sichuan. Rinse the collected samples with sterile water, weigh 1.0g of tissue, soak in 70% alcohol for 1-2min, then disinfect with 1-3% sodium hypochlorite solution for 3-5min, wash several times with sterile water, absorb Apply 100 μL of washing liquid for the last time to NA plate (3g beef extract, 10g peptone, 5g sodium chloride, 15-20g agar powder, 1000mL distilled water, pH 7.0, after mixing and dispensing, autoclave at 121°C for 30min), culture in the dark at 27°C 24h, used as a control for surface disinfection, to check whether the surface disinfection is thorough.

[0031] Cut the surface-sterilized bark tissue into pieces, put them into a sterile mortar, add sterilized quartz sand and 10mL of ster...

Embodiment 2

[0040] The preparation of embodiment 2 methylotrophic bacillus B18 liquid preparation

[0041] The methylotrophic bacillus B18 obtained according to embodiment 1 is made into a liquid preparation through seed culture and fermentation culture, which specifically includes the following steps:

[0042] 1) First-class slant seeds: Beef extract peptone slant medium was prepared by conventional methods, inoculated with methylotrophic Bacillus B18 strain and cultured at 26-28°C for 24-36 hours to make first-class seeds. The composition of its beef extract peptone slant medium is beef extract 7g, peptone 10g, NaCl 5g, agar 18g, water 1000mL.

[0043] 2) Secondary liquid seeds: After the nutrient fleshy culture solution is autoclaved, it is bottled according to the proportion of 100mL liquid in a 300mL triangular flask (controlled by oxygen), and inoculates the slant seeds of methylotrophic Bacillus B18 in a sterile state. The bottle was inoculated with 2 slant seeds, the temperature ...

Embodiment 3

[0047] Example 3 Prevention and growth-promoting experiment of methylotrophic Bacillus B18 on Eucommia

[0048] The experiment was carried out in the Forest Tree Germplasm Nursery of Sichuan Agricultural University. Eucommia ulmoides was managed routinely during the growth period, and the location of the flower pots was regularly rotated. The test soil was collected from Yangtianwo Drug Farm, Dayi County, Sichuan Province, with a sampling depth of 0-20cm, sandy loam. The physical and chemical properties of the soil are measured as follows: soil pH 7.1, organic matter content 11.54g / kg, total nitrogen content 0.76g / kg, total phosphorus content 0.42g / kg, available phosphorus content 4.71mg / kg. The sample soil was air-dried and passed through a 1mm sieve, and mixed with river sand passed through a 1mm sieve at a ratio of 3:1 (V:V) for pot experiments. After the eucommia seedlings were transplanted and survived, 100mL of the above-mentioned liquid bacterial agent was poured along...

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Abstract

The invention discloses bacillus methylotrophicus B18 as well as a liquid preparation prepared from the bacillus methylotrophicus B18 and application of the bacillus methylotrophicus B18. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 18, 2017 and has a preservation number of CGMCC No. 14641. The liquid preparation is prepared by taking thebacillus methylotrophicus B18 as the strain and carrying out seed cultivation and fermentation cultivation. The biocontrol bacterium-bacillus methylotrophicus B18 strain has double functions of disease prevention and growth promotion, has the characteristics of biological control and bio-fertilizer, and has remarkable effects on the control of damping-off of eucommia ulmoides and the promotion ofgrowth of the eucommia ulmoides; the bacillus methylotrophicus B18 not only can avoid the serious consequences of environmental pollution caused by chemical drugs, but also has the characteristics ofsustainability and low cost.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to the preparation of a methylotrophic bacillus B18 and its liquid preparation and its application in Eucommia damping-off and growth promotion. Background technique [0002] Eucommia ulmoides belongs to the Eucommia genus Eucommia perennial deciduous tree. There is only one genus and one species in this family. It is the only tertiary relic plant in my country. Eucommia has a cultivation history of nearly a thousand years and is extremely practical. It can be grown in 27 provinces from subtropical to temperate regions in my country, mainly in Sichuan, Yunnan, Guizhou, Hunan, Hubei, Shaanxi, Jiangsu and Zhejiang provinces. Eucommia ulmoides is listed as one of the demonstration varieties of Guizhou authentic Chinese medicinal materials GAP base. It is full of treasures, has a wide range of uses, and has great medicinal and economic value. Eucommia ulmoides is known as a magical tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 李姝江方馨玫朱天辉黎肇家徐永强谯天敏韩珊
Owner SICHUAN AGRI UNIV
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