1491 results about "Bacterial agent" patented technology

The invention relates to the technical field of microorganisms, in particular to application of a cladosporium spp. Strain and a synthetic flora thereof in plant growth promotion and bacterial wilt prevention and treatment, the cladosporium spp. HX2 is a new strain, has a good symbiotic relationship with tomatoes, has a good prevention effect on tomato bacterial wilt, and has a good application prospect in plant growth promotion and bacterial wilt prevention and treatment. After the bacterial strain is mixed with congeneric strains LJ1, MS2 and LC3 to prepare a cladosporium bacterial agent, the growth-promoting and disease-resisting capabilities of the bacterial agent are enhanced, and experiments show that the synthetic flora has a good growth-promoting function on tomatoes and fresh ginger, the yield of the fresh ginger is remarkably increased, and the growth vigor of tomato plants is improved; meanwhile, the synthetic flora also has a good prevention and treatment effect on bacterial wilt of tomatoes, fresh ginger and tobaccos, and can improve the bacterial wilt resistance of plants, so that the strain HX2 and the synthetic flora prepared from the strain HX2 can be used as an anti-microbial agent or a raw material for preparing a bio-organic fertilizer in the later period.

The invention provides a strain of Burkholderia graminicola as well as a bacterial agent and application of the Burkholderia graminicola. The preservation number of the Burkholderia graminicola is CCTCC (China Center For Type Culture Collection) NO: M20242399. The Burkholderia paragallinarum provided by the invention has the functions of nitrogen fixation, phosphate solubilization, siderophore production and indoleacetic acid production. The Burkholderia grassy and the fungicide taking the Burkholderia grassy as the effective active component can promote the germination of plant seeds and the growth of crops.

The invention provides a Pseudomonas protegens P150 strain and an application of the Pseudomonas protegens P150 strain, and belongs to the technical field of biocontrol bacteria. The defensive pseudomonas P150 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34520, the preservation time is May 13, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The defensive pseudomonas P150 disclosed by the invention has the effects of inhibiting various pathogenic bacteria, producing enzymes, promoting growth and preventing and treating tomato bacterial wilt and soybean pustule. The microbial inoculum prepared by adopting the biocontrol bacterium can reduce the dosage of chemical pesticides and the prevention and control cost of plant diseases, is green and pollution-free, can be used for preventing and controlling tomato bacterial wilt and soybean pustule, and has a relatively good popularization value.

The invention relates to the technical field of agricultural biology, and discloses an auxiliary cultivation method for promoting sugarcane biological nitrogen fixation, which comprises the following steps: (1) breeding a sugarcane variety with high nitrogen-fixing bacteria affinity, and preparing an environmental stress resistant nitrogen-fixing bacteria agent; (2) carrying out acidification improvement and carbon source pre-burying on the planting soil; (3) inoculating the nitrogen-fixing bacterial agent to the sugarcane seed stem or rhizosphere area; (4) maintaining the metabolic activity of nitrogen-fixing bacteria by dynamically regulating and controlling rhizosphere carbon source supply and oxygen partial pressure; (5) adjusting a fungicide supplementary application strategy in real time based on the environmental data; and (6) solidifying the symbiotic character through intergenerational epigenetic treatment. Through host-microorganism coevolution and intelligent dynamic regulation and control, the nitrogen fixation efficiency and environmental adaptability of the sugarcane-nitrogen-fixing bacteria symbiotic system are remarkably improved; and epigenetic and flora iteration technologies are combined, so that stable transmission of cross-generation characters is realized, and a low-consumption and high-benefit ecological solution is provided for sustainable agriculture.

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention provides an anti-premature senility management method for closely-planted double-layer truncated cone macadamia nut trees, and belongs to the technical field of fruit tree cultivation. The premature senility prevention management method comprises layered nutrition supply and flower and fruit protection management. The layered nutrition supply comprises the following steps: deeply applying a calcium magnesium phosphate fertilizer to the lower layer of soil greater than 60cm, and covering a root controller; organic fertilizers and biological agents are applied to 30-60 cm soil of the middle layer; a macroelement, humic acid and seaweed extract water-soluble fertilizer is applied to the 0-30 cm soil of the upper layer; the flower and fruit protection management comprises the steps that ABA and paclobutrazol are sprayed in the flower bud differentiation period; brassinolide and fluid boron are sprayed in the full-bloom stage; spraying forchlorfenuron and amino acid in a young fruit stage; nAA and monopotassium phosphate are sprayed in the fruit swelling period. Layered nutrition supply and flower and fruit protection management are carried out on the densely-planted double-layer truncated cone macadamia nut trees, premature senility of the densely-planted macadamia nut trees is prevented, branch development of the lower layer is promoted, the tree vigor is balanced, and the high-yield level is maintained.

The invention discloses a high-efficiency lespedeza growth-promoting bacillus Q4 (Bacillus atrophaeus) capable of improving the abundance of dominant bacteria and a preparation method of the high-efficiency lespedeza growth-promoting bacillus Q4. Belongs to the technical field of microorganisms. According to the bacillus atrophaeus Q4 provided by the invention, the conversion from total-effect nutrients to effective nutrients is improved, the consumption of lespedeza to soil nutrients is enhanced, and the abundance of dominant strain groups is increased, so that the effect of promoting the growth of lespedeza is achieved. After the bacillus atrophaeus Q4 provided by the invention is applied, the soil enzyme activity is remarkably improved, the conversion of available nutrients in soil is remarkably enhanced, and the nutrient consumption capability of lespedeza is enhanced. By applying the bacillus atrophaeus Q4, growth and development of lespedeza are promoted, the content of soil hydrolytic nitrogen and available phosphorus is remarkably increased, conversion to soil nutrients is improved, and the conductivity and organic carbon are increased to the maximum extent and are remarkably increased by 142.77% and 64.26% respectively. In the genus level, after being treated by the bacillus atrophaeus Q4, the bacillus (Bacillus) is obviously increased from 0.84% to 2.47%, and the average relative abundance of the pseudomonas (Fictibacillus) is obviously increased from 0.59% to 1.16%. By inoculating the Q4 inoculant, the structure of a soil colony is greatly improved, the relative abundance of a dominant flora is influenced, and the obvious increase of the relative abundance of Bacillus and Fictibacillus is possibly an important factor for promoting plant growth.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention discloses a complex microbial inoculant for promoting growth of leguminous plants and application of the complex microbial inoculant, and relates to the technical field of fertilizers and biologication.The complex microbial inoculant is prepared from 25-35 parts of a microbial inoculant, 10-20 parts of a biological active agent, 10-15 parts of a biological activator and 30-55 parts of water. The endophyte 3G1 which is screened and separated from rice seeds and can promote the growth of leguminous roots can be completely and successfully planted in plants by utilizing the effects of preventing and treating plant diseases and / or promoting plant growth of microorganisms, and the growth of the plants is directly influenced; in combination with the functions of low temperature resistance, plant disease resistance and the like of other complex microbial inoculants, the compound microbial inoculants have growth promoting and disease preventing effects on leguminous plants; the biological active agent is lipopeptide, so that the spreading performance of plant leaves can be improved, the absorption of the complex microbial inoculant is promoted, the activity of the microbial inoculant is improved, and the crop yield is promoted to be increased by 3%; the biological activator can provide trace elements in the plant growth process, and is matched with the complex microbial inoculants for use, so that the growth of crop seedlings can be effectively promoted, the stress resistance of the crop seedlings is improved, and the transplanting survival rate of the seedlings reaches up to 98%.

The invention relates to a complex microbial inoculant for preventing and treating citrus huanglongbing as well as a preparation method and application of the complex microbial inoculant. The compound bacterial agent for preventing and treating the citrus huanglongbing is prepared from the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. The compound bacterial agent for preventing and treating the citrus huanglongbing comprises the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. After the complex microbial inoculant is added, the content of callose in infected leaves can be effectively reduced, the self-resistance mechanism of plants is induced, the disease resistance is improved, and the complex microbial inoculant has an obvious inhibiting effect and a growth promoting effect on Candidatus Liberibacter asiaticum.

The invention belongs to the field of microorganisms. The invention provides a compound strain, a microbial inoculum and application of the compound strain in preparation of fermented East purple perilla tea. The compound strain comprises phytobacterium plantarum 2HDS3-La5 with the preservation number of CGMCC NO.34339, pediococcus acidilactici 2HDS3-SM6 with the preservation number of CGMCC NO.34337, Witzmania clostridium N2-La1 with the preservation number of CGMCC NO.34338, and the microbial inoculum is applied to preparation of fermented East purple perilla tea. The invention also provides application of the compound strain or a microbial inoculum containing the compound strain in preparation of fermented East purple perilla tea. The compound strain disclosed by the invention is applied to preparation of the fermented Dongbao tea, the content of substances with anti-inflammatory, anti-oxidation and immunoregulation effects in the Dongbao tea is remarkably increased by multiple times, and the prepared fermented Dongbao tea can remarkably improve the cell activity.

The invention relates to the field of environment and agricultural biotechnology, in particular to an application of a composite phosphate-solubilizing bacterial agent in promoting wheat growth, which is characterized in that germinated wheat is cultivated firstly, then the germinated wheat is placed in soil mixed with the composite phosphate-solubilizing bacterial agent to be cultivated, and then the germinated wheat is cultivated in soil mixed with the composite phosphate-solubilizing bacterial agent. Furthermore, the growth of wheat is promoted by the cooperation of enterobacter hormaechei HS6, pantoea agglomerans CT3 and wheat bran in the compound phosphate-solubilizing bacterium agent. Wherein the enterobacter hormaechei HS6 and the pantoea agglomerans CT3 can efficiently convert insoluble phosphorus in the soil into soluble phosphorus, the utilization rate of phosphorus in the soil is effectively increased, the use amount of chemical phosphate fertilizer is reduced, nutritional ingredients are increased for the soil, the physical and chemical properties of the soil are improved, wheat growth can be well promoted, and the application prospect is good.

The invention discloses an intestinal strain capable of effectively regulating uric acid metabolism, a microbial agent of the intestinal strain and application of the intestinal strain in uric acid reducing products, and belongs to the technical field of microorganisms. According to the enterococcus pluvialis ZQ205, the enterococcus pluvialis ZQ205 which is free of toxic and side effects and capable of effectively relieving and treating the hyperuricemia is found for the first time, and the collection number of the enterococcus pluvialis ZQ205 in the China Center for Type Culture Collection is CCTCC NO: M 2025844; the strain can significantly reduce the levels of uric acid, creatinine and urea nitrogen in serum of mice, can effectively reduce the levels of inflammatory factors such as IL-6 and TNF-alpha in serum of hyperuricemia mice, and can improve kidney injury caused by hyperuricemia.

The invention discloses a method for preparing a soil conditioner based on infrared activation of coal gangue. The method comprises the following steps: S1, preparing activated coal gangue; s2, mixing the activated coal gangue with a slow-release fertilizer and modified zeolite, performing extrusion granulation to obtain basic particles, diluting a long-acting composite microbial inoculum with deionized water to obtain a microbial inoculum suspension, spraying the microbial inoculum suspension on the surfaces of the basic particles in a low-temperature spraying manner, and performing low-temperature drying to obtain a core material; s3, mixing the activated coal gangue with kaolin, humic acid, calcium hydrogen phosphate, sodium alginate and water to obtain coating slurry, and coating the core material with the coating slurry to obtain a shell-core material; s4, mixing the modified activated coal gangue with polyvinyl alcohol, talcum powder, a composite additive and water to obtain outer coating slurry, and coating the outer coating slurry on the core-shell material to obtain the soil conditioner. According to the method, the coal gangue is subjected to infrared activation treatment and then prepared into the soil conditioner with a high additional value, and waste resource utilization is achieved.

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

The invention relates to bacillus amyloliquefaciens which is classified and named as bacillus amyloliquefaciens LDB1, the preservation number of the bacillus amyloliquefaciens is CCTCC No: M 20231897, and the preservation date is October 16, 2023. The invention relates to application of bacillus amyloliquefaciens in citrus waste compost. The invention relates to a microbial agent, which comprises bacillus amyloliquefaciens LDB1, bacillus amyloliquefaciens, bacillus subtilis and bacillus velezensis. The invention relates to application of a microbial agent in citrus waste compost. The method has the beneficial effects that lignin peroxidase, laccase and manganese peroxidase can be produced, good lignin degradation capability is achieved, degradation of lignocellulose in citrus waste compost can be effectively promoted by matching with other strains, the degradation rates of hemicellulose, cellulose and lignin are 33.8%, 36.5% and 38.5% respectively, and the method is suitable for industrial production. The strain and the microbial inoculum thereof have important application value in the aspect of recycling of high-lignin fiber organic wastes.

The invention discloses a Chinese chestnut composite phosphate-solubilizing bacterial agent and application thereof, and belongs to the technical field of microorganisms. The invention provides a compound phosphate-solubilizing bacterial agent. The compound phosphate-solubilizing bacterial agent is prepared from phosphate-solubilizing bacteria GXLK-W6 and phosphate-solubilizing fungi GXLK-WZ1-1. The phosphorus-solubilizing bacteria GXLK-WZ1-1 are classified and named as Aspergillus niger, the preservation number of the bacteria is CGMCC (China General Microbiological Culture Collection Center) No.41390, the phosphorus-solubilizing bacteria GXLK-W6 are classified as Burkholderia pyrrolothrix, and the preservation number of the bacteria is CGMCC No.31124. The phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1 and the phosphorus-solubilizing bacteria GXLK-WZ1-1 and The strains are independently separated from rhizosphere soil of Chinese chestnuts and are screened. And calcium phytate is added into the microbial inoculum to serve as a slow-release phosphorus source. The compound phosphate-solubilizing bacterial agent improves the growth amplification of Chinese chestnut seedlings, promotes the growth of root systems, and promotes the phosphorus absorption of plants. The compound phosphate-solubilizing bacterial agent can improve the dissolution efficiency of inorganic phosphorus in soil and increase the content of soluble available phosphorus, and plays an important role in improving the fertility of the soil.

The present invention provides a hydrangea oral liquid and a preparation method thereof, and relates to the field of biological fermentation technology. The preparation method of the hydrangea oral liquid of the present invention comprises: crushing the dried hydrangea fruiting bodies, mixing with glucose and water, ultrasonic treatment, obtaining a mixed material, inoculating a composite bacterial agent for fermentation, adding a composite enzyme for enzymolysis, filtering and concentrating, and obtaining the hydrangea oral liquid. The present invention combines hydrangea crushing with ultrasound, improves the dissolution of nutrients in hydrangea, and shortens the fermentation time; the composite bacterial agent fermentation can significantly increase the polysaccharide substances in the hydrangea oral liquid, and enhances the antioxidant effect; the composite bacterial agent fermentation of hydrangea and the composite enzyme enzymolysis can significantly improve the mushroom smell caused by the hydrangea itself. The experimental results show that the hydrangea oral liquid prepared by the method of the present invention can improve the mushroom smell caused by hydrangea, increase the polysaccharide content, and enhance the antioxidant performance.

The invention discloses a compound microbial agent for efficiently promoting attachment and metamorphosis of coral larvae and growth of the larvae and application of the compound microbial agent. The microbial agent comprises two strains, namely, Phytobacter sp.SCSIO43973 and Salipiger sp.SCSIO43974, and the preservation numbers of the two strains are respectively as follows: GDMCC (China General Microbiological Culture Collection Center) NO: 65583 and 65650. The two strains are separated from healthy coral deerhorn-shaped corals. Researches show that by adding the strains, the adhesion rate and the metamorphosis rate of planula larvae and the survival rate and the germination reproduction rate of coral larvae can be remarkably increased. The characteristics show that the microbial agent has the potential of improving the attachment metamorphosis rate of coral larvae and promoting growth in a field coral reef ecological system, and has important significance in increasing the larva supplement amount of the damaged coral reef ecological system and promoting the recovery and sustainable development of the coral reef ecological system. In conclusion, the microbial agent provided by the invention provides a new technical means for recovery of a coral reef ecosystem by promoting attachment and metamorphosis of coral larvae and growth of the larvae.

The invention discloses an algicidal bacterium and application thereof, the algicidal bacterium is named as Bacillus sp.L1.3, and is preserved in China Center for Type Culture Collection on March 31, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2025629. The nucleotide sequence of the 16S rRNA gene of the strain is as shown in SEQ ID NO: 1. The invention also provides application of the algicidal bacteria in treatment of water bloom of freshwater cyanobacteria, and the freshwater cyanobacteria is microcystis aeruginosa. Fermentation liquor of the strain L1.3 has a strong inhibition effect on growth of microcystis aeruginosa, secretions of the strain L1.3 can destroy cell walls, cell membranes, thylakoid and other structures of algae cells, inhibit photosynthesis of algae and cause irreversible damage to the algae, and the strain L1.3 has wide-range temperature stability and can be used for preparing the microcystis aeruginosa strain L1.3. The bacterial strain is suitable for being prepared into an algicidal bacterial agent to be applied to the field of biological control of freshwater cyanobacterial bloom.

The invention belongs to the field of fermentation, and particularly relates to a complex microbial inoculant, original cheese, processed cheese with a blood sugar reducing function and preparation and application of the complex microbial inoculant. Wherein the processed cheese is prepared by the method comprising the following steps: mixing the components including raw cheese, sugarcane juice, corn oil, casein, concentrated milk protein, blueberry powder, mono / diglycerol fatty acid ester, sodium citrate, sodium tripolyphosphate, sodium hexametaphosphate, salt guar gum, potassium sorbate and water, and then emulsifying to obtain the processed cheese, the sugar cane juice, the corn oil, the casein, the concentrated milk protein, the blueberry powder, the mono / diglycerol fatty acid ester, the sodium citrate, the sodium tripolyphosphate, the sodium hexametaphosphate, the salt guar gum, the potassium sorbate and the water, the original cheese is prepared from a complex microbial inoculant, and the complex microbial inoculant comprises lactococcus lactis bacterial powder, lactococcus lactis subsp. Lactis bacterial powder, lactobacillus delbrueckii subsp. Lactis bacterial powder, lactobacillus plantarum bacterial powder and lactobacillus paracasei bacterial powder. The processed cheese provided by the invention has a better function of controlling blood sugar, and meanwhile, the processed cheese is fine and uniform in texture and has good smearing performance.

The invention relates to the technical field of agricultural fertilizers, and particularly discloses a polypeptide fish protein flushing water-soluble fertilizer and a preparation method thereof, the water-soluble fertilizer is prepared from the following raw materials: unrotten small fish or fish sewer, edible cane sugar or brown granulated sugar, a self-prepared fish protein enzymolysis agent, a self-prepared fish protein anaerobic fermentation inoculant and water; the preparation method comprises an enzymolysis stage and a fermentation stage. The compound fertilizer can be used as a nitrogen fertilizer to replace a traditional chemical nitrogen fertilizer, or is matched with a trace element fertilizer in a compound fertilizer to be used in crop planting; the water-soluble fertilizer has the beneficial effects that the water-soluble fertilizer can supplement soil protein amino acid fertilizer nutrition and increase the content of nutritional ingredients in crops, fermented polypeptide micromolecule sugar can be directly absorbed by the crops to improve the taste, ecological organic planting is realized, meanwhile, the planting cost is reduced, and the water-soluble fertilizer is suitable for fruit and vegetable planting families, farms, picking gardens, greenhouse farmers and the like, is simple and easy to operate, and is suitable for popularization and application. Raw materials are easy to collect, low in cost and obvious in effect.

The invention discloses an application of Lactobacillus plantarum in at least one of the following items: a1, an application in improving the silage quality of silage, a2, an application in improving the silage quality, a3, an application in improving the silage quality, a4, an application in improving the silage quality, and a5, an application in improving the silage quality. A2, application in preparation of silage; a3, application in preparation of a silage additive; the strain name of the lactobacillus plantarum is LA2, the lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lactobacillus plantarum is CGMCC No. 33484. According to the invention, the lactobacillus plantarum or the microbial inoculum thereof or the silage additive thereof is added into the silage for the first time, and tetracycline resistance genes in the silage can be remarkably reduced; after the lactobacillus plantarum or the microbial inoculum thereof or the silage additive thereof disclosed by the invention is added, the generation and residue of tetracycline resistance genes in feed can be reduced, the propagation of the tetracycline resistance genes in a food chain is reduced, the safety of forage grass is ensured, and the damage to animals, environment and human bodies is reduced; and the drug resistance of human pathogenic bacteria is reduced while the health level of animals is improved.

The invention provides a complex microbial inoculant as well as a preparation method and application thereof, and relates to the technical field of complex microbial inoculant preparation. The compound bacterial agent is prepared from bacillus pumilus JMB004, bacillus safeensis strain BXC22 and bacillus sp. EB35, and the compound bacterial agent is prepared from bacillus pumilus strain JMB004, bacillus safeensis strain BXC22 and bacillus sp. EB35. The three strains are obtained through autonomous screening and prepared into the complex microbial inoculant, dry powder can be stored at normal temperature, the storage life is prolonged to one year, and the composite microbial inoculant is convenient to carry. The complex microbial inoculant is only composed of three strains of bacteria, and the components are simple; the low-temperature composting agent is applied to composting treatment under a low-temperature condition, and is relatively high in temperature rise rate, relatively short in fermentation time and relatively high in decomposition degree.

The invention discloses a method for preparing a liquid fertilizer and improving crop yield by using tylosin waste liquid, and belongs to the technical field of agricultural waste resource utilization, and the method comprises the following steps: S1, preparing a novel liquid fertilizer: mixing the tylosin waste liquid with an antibiotic degradation agent for reaction, centrifuging, and collecting supernatant; adding nutrient substances and a mixed bacterial agent into the centrifugate for fermentation treatment, and filtering to obtain filtrate, namely the novel liquid fertilizer; s2, taking crop seeds, and soaking the crop seeds in a soaking solution before planting; s3, in the seed bud stage and the seedling stage, leaf surface spraying is conducted; wherein the antibiotic degradation agent is prepared by reacting modified silicon dioxide with sulfhydrylated sodium carboxymethyl cellulose. According to the method, resource utilization of the tylosin waste liquid is achieved, environmental pollution and use of chemical fertilizers are reduced, the agricultural production cost is reduced, and an effective way is provided for agricultural sustainable development.

The invention belongs to the field of microbial preparations, and particularly relates to a complex microbial inoculant for promoting microbial quorum sensing and application of the complex microbial inoculant in compost. The composite microbial agent comprises pseudomonas A3 and paenibacillus polymyxa 21-2, and the preservation number of the pseudomonas A3 is CCTCC (China Center for Type Culture Collection) NO: M20251394. The pseudomonas A3 can express key genes such as rhlI and lasI of a gram-negative bacteria AHL quorum sensing system and signal molecules of the key genes, and the paenibacillus polymyxa 21-2 can express luxS genes of a gram-positive bacteria AI-2 signal system and signal molecules DPD of the luxS genes. In the pig manure compost, the complex microbial inoculant is added, so that the expression of AI-2 and AHLs system related genes in the compost and the content of signal molecules DPD are remarkably improved, the microbial population sensing process of the compost is promoted, finally, the compost temperature is increased by about 6 DEG C, the compost carbon-nitrogen ratio is reduced, and the compost maturity is promoted.

The invention discloses a sphingomonas sphingomonas, a bacterial agent thereof and application of the sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas The sphingomonas disclosed by the invention has excellent saline-alkaline tolerance, has the functions of fixing nitrogen, decomposing organic phosphorus and producing ACC deaminase, breaks through the limitation of single function of a traditional strain, and has a very good application prospect in saline-alkaline soil improvement, plant growth promotion and ecological restoration.

The present invention belongs to the technical fields of biological bacterium agents and functional food processing. Specifically, it relates to a bacterium agent for soybean milk fermentation and its application, a soy cheese stick and a preparation method thereof. The bacterium agent for soybean milk fermentation provided by the present invention uses Lactobacillus helveticus HL88 and Lactobacillus casei BL25 in combination. It can not only quickly acidify the soy milk, shortening the coagulation time of soy cheese, but also more fully hydrolyze soy proteins, releasing more short peptides and amino acids. In particular, it can highly produce γ-aminobutyric acid (GABA), enhancing the fermentation effect and nutritional value of soybean milk. Further, the soy cheese food prepared by fermenting with the above bacterium agent not only has a strong soy milk fragrance and a smooth texture, but is also easily digested and absorbed, improving the digestibility and reducing the soybean allergenicity, especially helping to improve exercise fatigue and enhance sleep quality. Therefore, the above bacterium agent provided by the present invention has excellent application prospects in the field of preparing functional foods for anti-fatigue and promoting sleep.

The invention belongs to the technical field of antibacterial active microorganisms, and particularly relates to a strain of diffusion carbon pad bacteria LUCF 4343-3, a bacterial agent and application of the bacterial agent in preparation of an antibacterial preparation. According to the invention, an endophytic fungus strain with remarkable antibacterial potential is successfully separated from Parmotrema, and the endophytic fungus strain is identified as diffusion carbon pad fungus LUCF 4343-3. Experiments show that an ethyl acetate extract of the strain shows a good inhibition effect on various clinical common pathogenic bacteria, including gram-positive bacteria (staphylococcus aureus and enterococcus faecalis), gram-negative bacteria (pseudomonas aeruginosa), fungi (candida albicans and candida glabrata) and the like; the discovery provides an important basis for the application of the strain in the development of novel broad-spectrum antibacterial drugs.

The invention discloses a functional probiotic composition and application thereof, and relates to the technical field of microbial engineering. Bifidobacterium longum, lactobacillus acidophilus and enterococcus faecalis are combined to serve as a microbial agent, the microbial agent and active substances of the tea leaves form a composition, then the composition is combined with prebiotics, it is ensured that viable bacteria directly reach the intestinal tract through a microencapsulation technology, and the effect of improving the immune function is achieved; tea polyphenol in the active substances of the tea leaves can serve as a carbon source of prebiotics to stimulate proliferation of the bifidobacterium longum and the lactobacillus acidophilus, so that the acid resistance and intestinal colonization capacity of the microbial inoculum are enhanced, and meanwhile, theanine in the active substances of the tea leaves, the bifidobacterium longum and the lactobacillus acidophilus synergistically promote secretion of gamma-aminobutyric acid, so that the effect of improving the intestinal colonization is achieved. And the tea polyphenol can inhibit the large intestine rod from adhering to the intestinal mucosa, and the probiotic agent secretes antibacterial peptide, so that the integrity of the intestinal barrier is maintained together.