1255 results about "Bacterial agent" patented technology

The invention provides a Pseudomonas protegens P150 strain and an application of the Pseudomonas protegens P150 strain, and belongs to the technical field of biocontrol bacteria. The defensive pseudomonas P150 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34520, the preservation time is May 13, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The defensive pseudomonas P150 disclosed by the invention has the effects of inhibiting various pathogenic bacteria, producing enzymes, promoting growth and preventing and treating tomato bacterial wilt and soybean pustule. The microbial inoculum prepared by adopting the biocontrol bacterium can reduce the dosage of chemical pesticides and the prevention and control cost of plant diseases, is green and pollution-free, can be used for preventing and controlling tomato bacterial wilt and soybean pustule, and has a relatively good popularization value.

The invention relates to the technical field of agricultural biology, and discloses an auxiliary cultivation method for promoting sugarcane biological nitrogen fixation, which comprises the following steps: (1) breeding a sugarcane variety with high nitrogen-fixing bacteria affinity, and preparing an environmental stress resistant nitrogen-fixing bacteria agent; (2) carrying out acidification improvement and carbon source pre-burying on the planting soil; (3) inoculating the nitrogen-fixing bacterial agent to the sugarcane seed stem or rhizosphere area; (4) maintaining the metabolic activity of nitrogen-fixing bacteria by dynamically regulating and controlling rhizosphere carbon source supply and oxygen partial pressure; (5) adjusting a fungicide supplementary application strategy in real time based on the environmental data; and (6) solidifying the symbiotic character through intergenerational epigenetic treatment. Through host-microorganism coevolution and intelligent dynamic regulation and control, the nitrogen fixation efficiency and environmental adaptability of the sugarcane-nitrogen-fixing bacteria symbiotic system are remarkably improved; and epigenetic and flora iteration technologies are combined, so that stable transmission of cross-generation characters is realized, and a low-consumption and high-benefit ecological solution is provided for sustainable agriculture.

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention provides an anti-premature senility management method for closely-planted double-layer truncated cone macadamia nut trees, and belongs to the technical field of fruit tree cultivation. The premature senility prevention management method comprises layered nutrition supply and flower and fruit protection management. The layered nutrition supply comprises the following steps: deeply applying a calcium magnesium phosphate fertilizer to the lower layer of soil greater than 60cm, and covering a root controller; organic fertilizers and biological agents are applied to 30-60 cm soil of the middle layer; a macroelement, humic acid and seaweed extract water-soluble fertilizer is applied to the 0-30 cm soil of the upper layer; the flower and fruit protection management comprises the steps that ABA and paclobutrazol are sprayed in the flower bud differentiation period; brassinolide and fluid boron are sprayed in the full-bloom stage; spraying forchlorfenuron and amino acid in a young fruit stage; nAA and monopotassium phosphate are sprayed in the fruit swelling period. Layered nutrition supply and flower and fruit protection management are carried out on the densely-planted double-layer truncated cone macadamia nut trees, premature senility of the densely-planted macadamia nut trees is prevented, branch development of the lower layer is promoted, the tree vigor is balanced, and the high-yield level is maintained.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention belongs to the field of microorganisms. The invention provides a compound strain, a microbial inoculum and application of the compound strain in preparation of fermented East purple perilla tea. The compound strain comprises phytobacterium plantarum 2HDS3-La5 with the preservation number of CGMCC NO.34339, pediococcus acidilactici 2HDS3-SM6 with the preservation number of CGMCC NO.34337, Witzmania clostridium N2-La1 with the preservation number of CGMCC NO.34338, and the microbial inoculum is applied to preparation of fermented East purple perilla tea. The invention also provides application of the compound strain or a microbial inoculum containing the compound strain in preparation of fermented East purple perilla tea. The compound strain disclosed by the invention is applied to preparation of the fermented Dongbao tea, the content of substances with anti-inflammatory, anti-oxidation and immunoregulation effects in the Dongbao tea is remarkably increased by multiple times, and the prepared fermented Dongbao tea can remarkably improve the cell activity.

The invention discloses an intestinal strain capable of effectively regulating uric acid metabolism, a microbial agent of the intestinal strain and application of the intestinal strain in uric acid reducing products, and belongs to the technical field of microorganisms. According to the enterococcus pluvialis ZQ205, the enterococcus pluvialis ZQ205 which is free of toxic and side effects and capable of effectively relieving and treating the hyperuricemia is found for the first time, and the collection number of the enterococcus pluvialis ZQ205 in the China Center for Type Culture Collection is CCTCC NO: M 2025844; the strain can significantly reduce the levels of uric acid, creatinine and urea nitrogen in serum of mice, can effectively reduce the levels of inflammatory factors such as IL-6 and TNF-alpha in serum of hyperuricemia mice, and can improve kidney injury caused by hyperuricemia.

The invention discloses a method for preparing a soil conditioner based on infrared activation of coal gangue. The method comprises the following steps: S1, preparing activated coal gangue; s2, mixing the activated coal gangue with a slow-release fertilizer and modified zeolite, performing extrusion granulation to obtain basic particles, diluting a long-acting composite microbial inoculum with deionized water to obtain a microbial inoculum suspension, spraying the microbial inoculum suspension on the surfaces of the basic particles in a low-temperature spraying manner, and performing low-temperature drying to obtain a core material; s3, mixing the activated coal gangue with kaolin, humic acid, calcium hydrogen phosphate, sodium alginate and water to obtain coating slurry, and coating the core material with the coating slurry to obtain a shell-core material; s4, mixing the modified activated coal gangue with polyvinyl alcohol, talcum powder, a composite additive and water to obtain outer coating slurry, and coating the outer coating slurry on the core-shell material to obtain the soil conditioner. According to the method, the coal gangue is subjected to infrared activation treatment and then prepared into the soil conditioner with a high additional value, and waste resource utilization is achieved.

The invention discloses a Chinese chestnut composite phosphate-solubilizing bacterial agent and application thereof, and belongs to the technical field of microorganisms. The invention provides a compound phosphate-solubilizing bacterial agent. The compound phosphate-solubilizing bacterial agent is prepared from phosphate-solubilizing bacteria GXLK-W6 and phosphate-solubilizing fungi GXLK-WZ1-1. The phosphorus-solubilizing bacteria GXLK-WZ1-1 are classified and named as Aspergillus niger, the preservation number of the bacteria is CGMCC (China General Microbiological Culture Collection Center) No.41390, the phosphorus-solubilizing bacteria GXLK-W6 are classified as Burkholderia pyrrolothrix, and the preservation number of the bacteria is CGMCC No.31124. The phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1 and the phosphorus-solubilizing bacteria GXLK-WZ1-1 and The strains are independently separated from rhizosphere soil of Chinese chestnuts and are screened. And calcium phytate is added into the microbial inoculum to serve as a slow-release phosphorus source. The compound phosphate-solubilizing bacterial agent improves the growth amplification of Chinese chestnut seedlings, promotes the growth of root systems, and promotes the phosphorus absorption of plants. The compound phosphate-solubilizing bacterial agent can improve the dissolution efficiency of inorganic phosphorus in soil and increase the content of soluble available phosphorus, and plays an important role in improving the fertility of the soil.

The invention discloses an algicidal bacterium and application thereof, the algicidal bacterium is named as Bacillus sp.L1.3, and is preserved in China Center for Type Culture Collection on March 31, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2025629. The nucleotide sequence of the 16S rRNA gene of the strain is as shown in SEQ ID NO: 1. The invention also provides application of the algicidal bacteria in treatment of water bloom of freshwater cyanobacteria, and the freshwater cyanobacteria is microcystis aeruginosa. Fermentation liquor of the strain L1.3 has a strong inhibition effect on growth of microcystis aeruginosa, secretions of the strain L1.3 can destroy cell walls, cell membranes, thylakoid and other structures of algae cells, inhibit photosynthesis of algae and cause irreversible damage to the algae, and the strain L1.3 has wide-range temperature stability and can be used for preparing the microcystis aeruginosa strain L1.3. The bacterial strain is suitable for being prepared into an algicidal bacterial agent to be applied to the field of biological control of freshwater cyanobacterial bloom.

The invention belongs to the field of fermentation, and particularly relates to a complex microbial inoculant, original cheese, processed cheese with a blood sugar reducing function and preparation and application of the complex microbial inoculant. Wherein the processed cheese is prepared by the method comprising the following steps: mixing the components including raw cheese, sugarcane juice, corn oil, casein, concentrated milk protein, blueberry powder, mono / diglycerol fatty acid ester, sodium citrate, sodium tripolyphosphate, sodium hexametaphosphate, salt guar gum, potassium sorbate and water, and then emulsifying to obtain the processed cheese, the sugar cane juice, the corn oil, the casein, the concentrated milk protein, the blueberry powder, the mono / diglycerol fatty acid ester, the sodium citrate, the sodium tripolyphosphate, the sodium hexametaphosphate, the salt guar gum, the potassium sorbate and the water, the original cheese is prepared from a complex microbial inoculant, and the complex microbial inoculant comprises lactococcus lactis bacterial powder, lactococcus lactis subsp. Lactis bacterial powder, lactobacillus delbrueckii subsp. Lactis bacterial powder, lactobacillus plantarum bacterial powder and lactobacillus paracasei bacterial powder. The processed cheese provided by the invention has a better function of controlling blood sugar, and meanwhile, the processed cheese is fine and uniform in texture and has good smearing performance.

The invention relates to the technical field of agricultural fertilizers, and particularly discloses a polypeptide fish protein flushing water-soluble fertilizer and a preparation method thereof, the water-soluble fertilizer is prepared from the following raw materials: unrotten small fish or fish sewer, edible cane sugar or brown granulated sugar, a self-prepared fish protein enzymolysis agent, a self-prepared fish protein anaerobic fermentation inoculant and water; the preparation method comprises an enzymolysis stage and a fermentation stage. The compound fertilizer can be used as a nitrogen fertilizer to replace a traditional chemical nitrogen fertilizer, or is matched with a trace element fertilizer in a compound fertilizer to be used in crop planting; the water-soluble fertilizer has the beneficial effects that the water-soluble fertilizer can supplement soil protein amino acid fertilizer nutrition and increase the content of nutritional ingredients in crops, fermented polypeptide micromolecule sugar can be directly absorbed by the crops to improve the taste, ecological organic planting is realized, meanwhile, the planting cost is reduced, and the water-soluble fertilizer is suitable for fruit and vegetable planting families, farms, picking gardens, greenhouse farmers and the like, is simple and easy to operate, and is suitable for popularization and application. Raw materials are easy to collect, low in cost and obvious in effect.

The invention discloses an application of Lactobacillus plantarum in at least one of the following items: a1, an application in improving the silage quality of silage, a2, an application in improving the silage quality, a3, an application in improving the silage quality, a4, an application in improving the silage quality, and a5, an application in improving the silage quality. A2, application in preparation of silage; a3, application in preparation of a silage additive; the strain name of the lactobacillus plantarum is LA2, the lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lactobacillus plantarum is CGMCC No. 33484. According to the invention, the lactobacillus plantarum or the microbial inoculum thereof or the silage additive thereof is added into the silage for the first time, and tetracycline resistance genes in the silage can be remarkably reduced; after the lactobacillus plantarum or the microbial inoculum thereof or the silage additive thereof disclosed by the invention is added, the generation and residue of tetracycline resistance genes in feed can be reduced, the propagation of the tetracycline resistance genes in a food chain is reduced, the safety of forage grass is ensured, and the damage to animals, environment and human bodies is reduced; and the drug resistance of human pathogenic bacteria is reduced while the health level of animals is improved.

The invention belongs to the field of microbial preparations, and particularly relates to a complex microbial inoculant for promoting microbial quorum sensing and application of the complex microbial inoculant in compost. The composite microbial agent comprises pseudomonas A3 and paenibacillus polymyxa 21-2, and the preservation number of the pseudomonas A3 is CCTCC (China Center for Type Culture Collection) NO: M20251394. The pseudomonas A3 can express key genes such as rhlI and lasI of a gram-negative bacteria AHL quorum sensing system and signal molecules of the key genes, and the paenibacillus polymyxa 21-2 can express luxS genes of a gram-positive bacteria AI-2 signal system and signal molecules DPD of the luxS genes. In the pig manure compost, the complex microbial inoculant is added, so that the expression of AI-2 and AHLs system related genes in the compost and the content of signal molecules DPD are remarkably improved, the microbial population sensing process of the compost is promoted, finally, the compost temperature is increased by about 6 DEG C, the compost carbon-nitrogen ratio is reduced, and the compost maturity is promoted.

The invention discloses a sphingomonas sphingomonas, a bacterial agent thereof and application of the sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas The sphingomonas disclosed by the invention has excellent saline-alkaline tolerance, has the functions of fixing nitrogen, decomposing organic phosphorus and producing ACC deaminase, breaks through the limitation of single function of a traditional strain, and has a very good application prospect in saline-alkaline soil improvement, plant growth promotion and ecological restoration.

The invention belongs to the technical field of antibacterial active microorganisms, and particularly relates to a strain of diffusion carbon pad bacteria LUCF 4343-3, a bacterial agent and application of the bacterial agent in preparation of an antibacterial preparation. According to the invention, an endophytic fungus strain with remarkable antibacterial potential is successfully separated from Parmotrema, and the endophytic fungus strain is identified as diffusion carbon pad fungus LUCF 4343-3. Experiments show that an ethyl acetate extract of the strain shows a good inhibition effect on various clinical common pathogenic bacteria, including gram-positive bacteria (staphylococcus aureus and enterococcus faecalis), gram-negative bacteria (pseudomonas aeruginosa), fungi (candida albicans and candida glabrata) and the like; the discovery provides an important basis for the application of the strain in the development of novel broad-spectrum antibacterial drugs.

The invention discloses a functional probiotic composition and application thereof, and relates to the technical field of microbial engineering. Bifidobacterium longum, lactobacillus acidophilus and enterococcus faecalis are combined to serve as a microbial agent, the microbial agent and active substances of the tea leaves form a composition, then the composition is combined with prebiotics, it is ensured that viable bacteria directly reach the intestinal tract through a microencapsulation technology, and the effect of improving the immune function is achieved; tea polyphenol in the active substances of the tea leaves can serve as a carbon source of prebiotics to stimulate proliferation of the bifidobacterium longum and the lactobacillus acidophilus, so that the acid resistance and intestinal colonization capacity of the microbial inoculum are enhanced, and meanwhile, theanine in the active substances of the tea leaves, the bifidobacterium longum and the lactobacillus acidophilus synergistically promote secretion of gamma-aminobutyric acid, so that the effect of improving the intestinal colonization is achieved. And the tea polyphenol can inhibit the large intestine rod from adhering to the intestinal mucosa, and the probiotic agent secretes antibacterial peptide, so that the integrity of the intestinal barrier is maintained together.

The invention discloses Pseudomonas sp., a microbial agent prepared from the Pseudomonas sp. And application of the Pseudomonas sp. And the microbial agent prepared from the Pseudomonas sp. The pseudomonas strain N2-3-1-14 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC); the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation time is April 8, 2025; the preservation number is CGMCC 34128. The pseudomonas strain N2-3-1-14 and the microbial inoculum prepared from the pseudomonas strain N2-3-1-14 can effectively inhibit growth of pathogenic bacteria of pine blight and pine needle red spot, and the bacteriostasis rate is 65% or above. The strain can also significantly promote the growth of pinus sylvestris.

The invention discloses a complex microbial inoculant for treating underground water as well as a preparation method and application thereof, relates to the field of sewage treatment, and aims to solve the problem that TBBPA and nitrate in underground water are difficult to remove at the same time in an existing biological treatment method. The composite microbial agent is prepared from pseudomonas koreensis Y5-11, acinetobacter pidermatum LYS-5, Pseudomonas sp. Y39-6 and psychrophilic bacteria F5-6, and the pseudomonas sp. Y5-11, the acinetobacter pidermatum LYS-5, the Pseudomonas sp. Y39-6, the psychrophilic bacteria F5-6 and the psychrophilic bacteria F5-6 are added into the microbial agent. The four strains are respectively subjected to fermentation culture, and the four fermentation liquids are mixed to prepare the complex microbial inoculant. The four bacteria in the complex microbial inoculant have a stronger metabolism complementation effect, and the effect on composite pollution remediation is more remarkable. The complex microbial inoculant is used for removing TBBPA and nitrate in underground water at the same time, the complex microbial inoculant has biodiversity, and the treatment result conforms to the water quality characteristics of the underground water.

The invention provides paracoccus pantotrophus, a deodorant composed of the paracoccus pantotrophus and application of the deodorant, and belongs to the technical field of microbial agents. According to the invention, a paracoccus pantotrophus strain is screened from biogas residues of a pig farm, is named as Paracoccus pantotrophus SZZ1 and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, and the biological preservation number is CCTCC M20242509. The SZZ1 strain is used for animal manure fermentation, and results show that the microbial inoculum can effectively reduce odor generated during manure fermentation, and especially has a significant inhibition effect on release of hydrogen sulfide. Therefore, the method not only enriches the types of existing deodorization strains, but also has an excellent removal effect on hydrogen sulfide and a stable and lasting deodorization effect, and is suitable for popularization and application.

The invention relates to the technical field of soil remediation, and provides a soil pollution remediation process which comprises the following steps: distributing points in grids of an area to be remediated, measuring the content of heavy metals and the concentration of organic pollutants, applying multi-frequency microwave radiation, synchronously applying ultrasonic treatment, and injecting a complex microbial inoculant containing sphingomonas, aspergillus niger spores and a nitrogen and phosphorus nutrient solution. A direct-current electric field is applied, pH, oxidation-reduction potential Eh and temperature parameters are monitored and measured in real time through an embedded sensor, a repair by-product recovery system is arranged, the purity of recovered metal is not lower than 98%, and mobile repair equipment is used in a matched mode. According to the method, the pollution degree is accurately quantified by constructing the pollution index PI, and dynamic regulation and repair are realized in combination with real-time monitoring parameters of a sensor. The pollutant removal efficiency is improved through the synergistic effect of multiple technologies, metal resource recovery is achieved through a repair by-product recovery system, traditional problems are solved, and efficient and environment-friendly repair is achieved.

The invention provides a spiropolyspora roseus strain for preventing and treating root knot nematode disease, and the preservation number of the spiropolyspora roseus strain is CGMCC No.41822. The invention also provides a microbial inoculum containing the spiropolyspora pink strain, and the microbial inoculum is prepared by the following steps: inoculating a culture medium with the spiropolyspora pink HLF-14-11, and culturing until spores are produced; spores are eluted to prepare a spore suspension, or a bacterial block is cut to serve as a liquid fermentation inoculant; inoculating the inoculant into a liquid culture medium, and carrying out shake culture; and taking the fermentation liquor to prepare liquid, powder and granules. The spiropolyspora pink strain provided by the invention is separated from root-knot nematode oocysts of diseased tomatoes, can parasitize eggs of root-knot nematodes and inhibit incubation of the eggs, fermentation filtrate of the spiropolyspora pink strain has a relatively strong poisoning effect on second-stage larvae of the root-knot nematodes, and the fatality rate of the second-stage larvae reaches 89.1%. Meanwhile, the strain can produce auxin substances such as indoleacetic acid and the like, and plant growth and root development are promoted.

The invention belongs to the field of food microorganisms, and particularly relates to a saccharomycopsis fibuligera strain, a microbial inoculum, a fermented product and preparation and application thereof. The saccharomycopsis fibuligera strain provided by the invention is preserved in the China Center for Type Culture Collection on September 25, 2023, and the preservation number of the saccharomycopsis fibuligera strain is CCTCC NO: M 20231788. The saccharomycopsis fibuligera strain provided by the invention can produce amylase, protease, cellulase and other enzymes required by fermentation, the amylase has the activity of pullulanase and raw amylase and can well adapt to the special environmental conditions of fermentation, and the saccharomycopsis fibuligera strain has high environmental tolerance and high aroma-producing capacity; therefore, the quality of fermented products is improved, and the fermentation production investment cost is reduced.

The invention relates to a lake sediment reduction bacterium which is classified and named as bacillus amyloliquefaciens WP02, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2020696, and the lake sediment reduction bacterium is preserved in the China Center for Type Culture Collection. The invention relates to an application of a lake sediment reduction bacterium in sediment denitrification, phosphorus uptake and quality degradation. The method has the beneficial effects that the immobilized microbial agent obtained by respectively embedding and adsorbing lake sediment reduction bacteria can effectively reduce sediment organic matters, reduce sediment total nitrogen and absorb phosphorus in overlying water, namely effectively perform denitrification, phosphorus absorption and quality degradation on the sediment, and the microbial agent can improve the oxidation reduction potential of the sediment and reduce the pollution of the sediment. And the method has important significance and application value in the aspect of maintaining the ecological balance of the water body, and has the characteristics of high in-situ remediation efficiency, small environmental disturbance and no secondary pollution compared with a physical and chemical remediation method.

The invention provides a section reconstruction structure and method for coal gangue artificial soil, and the method comprises the steps: carrying out surface soil layering treatment on a land reclamation area, laying coarse-grained gangue at the bottom of the land reclamation area to form a coarse-grained gangue layer, and the particle size of the coarse-grained gangue is 4-6cm; coal gangue artificial soil covers the coarse-grain gangue layer to form a coal gangue artificial soil layer, and the thickness of the coarse-grain gangue layer is smaller than that of the coal gangue artificial soil layer. The coal gangue artificial soil comprises coal gangue, local soil, organic fertilizer, biochar and fungicide, and the mass ratio of the coal gangue to the coal gangue artificial soil layer is 60%. The coal gangue soil utilization benefit of the mining area can be improved, the cost can be saved while the crop growth effect is guaranteed, and the method is easy to operate, practical and low in cost.

The invention belongs to the field of microorganisms, and particularly relates to a biochar-based immobilized microbial agent for promoting litter decomposition and a preparation method thereof.The biochar-based immobilized microbial agent for promoting litter decomposition comprises a biochar-based culture medium and a microbial seed solution, the active ingredient of the microbial seed solution is phanerochaete chrysosporium, and the preservation number is SHBCC D13411. According to the invention, the adsorption efficiency of the microbial agent is enhanced through the iron-modified biochar, and a habitat suitable for development and reproduction is provided for microorganisms; on the other hand, the biochar has very strong hygroscopicity, water molecules can be adsorbed on the surface, and a suitable growth environment is provided for microorganisms, so that the microorganisms are promoted to decompose litters.

The invention discloses a special bacterial fertilizer for sorghum and application of the special bacterial fertilizer in prevention and control of sorghum diseases, and belongs to the technical field of microbial fertilizers and agriculture. The special bacterial fertilizer for sorghum comprises the following components in parts by weight: 8-10 parts of a composite microbial agent, 40-50 parts of sorghum residues, 5-10 parts of bentonite, 1-2 parts of potassium humate, 1-2 parts of composite trace elements, 10-20 parts of sawdust and 10-20 parts of rice bran, wherein the complex microbial inoculant consists of a bacillus subtilis microbial inoculant, a rhizobium microbial inoculant, a bacillus velezensis microbial inoculant and a trichoderma harzianum microbial inoculant. The special bacterial fertilizer for sorghum provided by the invention contains rich microorganisms and nutrient substances, and can effectively prevent and control sorghum diseases and enhance the disease resistance of sorghum. The components such as microorganisms and organic acids in the special bacterial fertilizer for sorghum can promote the formation of soil organic matters, improve the soil structure, improve the soil quality and effectively improve the growth environment of sorghum.

The invention belongs to the technical field of microorganisms, and particularly relates to a Prussia adamsii T54 strain, a microbial agent and application of the Prussia adamsii T54 strain and the microbial agent. The Pichia aryabhattai T54 is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on February 21, 2025, and the preservation number of the Pichia aryabhattai T54 is CGMCC (China General Microbiological Culture Collection Center) No. 33613. According to the present invention, the Princisteria aryabhattai T54 and the bacterial agent have significant inhibition effects on a variety of plant pathogenic fungi, and further have plant growth promoting effects;

The invention discloses a method for preparing boxthorn leaf Kangpu tea with high antioxidant activity by using an artificial microbial agent, and belongs to the field of food processing. The boxthorn leaf kangpu tea is prepared through fermentation of the artificially synthesized microbial agent, the fermentation method is easy to operate and short in fermentation time, the antioxidant activity of the kangpu tea is improved, the flavor and the health-care effect of boxthorn leaves are reserved to the maximum extent, agricultural resources are fully utilized, the environment is protected, and economic benefits and social benefits are improved.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus agave, fermentation liquor, a biological agent and application. The invention discloses a strain of bacillus tequilensis, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus tequilensis is CGMCC NO.34830. The strain of bacillus tequilensis has the advantages that the preservation number is CGMCC NO.34830; the invention also discloses a fermentation broth and a biological agent prepared according to the bacillus agave, and the bacillus agave, the fermentation broth and the biological agent provided by the invention can effectively reduce the harm of root knot nematode disease to plants and promote seed germination or crop growth, and have the advantages of high efficiency, safety, environmental protection and difficult generation of resistance.