900 results about "Bacterial agent" patented technology

The invention provides a Pseudomonas protegens P150 strain and an application of the Pseudomonas protegens P150 strain, and belongs to the technical field of biocontrol bacteria. The defensive pseudomonas P150 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34520, the preservation time is May 13, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The defensive pseudomonas P150 disclosed by the invention has the effects of inhibiting various pathogenic bacteria, producing enzymes, promoting growth and preventing and treating tomato bacterial wilt and soybean pustule. The microbial inoculum prepared by adopting the biocontrol bacterium can reduce the dosage of chemical pesticides and the prevention and control cost of plant diseases, is green and pollution-free, can be used for preventing and controlling tomato bacterial wilt and soybean pustule, and has a relatively good popularization value.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention belongs to the field of microorganisms. The invention provides a compound strain, a microbial inoculum and application of the compound strain in preparation of fermented East purple perilla tea. The compound strain comprises phytobacterium plantarum 2HDS3-La5 with the preservation number of CGMCC NO.34339, pediococcus acidilactici 2HDS3-SM6 with the preservation number of CGMCC NO.34337, Witzmania clostridium N2-La1 with the preservation number of CGMCC NO.34338, and the microbial inoculum is applied to preparation of fermented East purple perilla tea. The invention also provides application of the compound strain or a microbial inoculum containing the compound strain in preparation of fermented East purple perilla tea. The compound strain disclosed by the invention is applied to preparation of the fermented Dongbao tea, the content of substances with anti-inflammatory, anti-oxidation and immunoregulation effects in the Dongbao tea is remarkably increased by multiple times, and the prepared fermented Dongbao tea can remarkably improve the cell activity.

The invention relates to the technical field of agricultural fertilizers, and particularly discloses a polypeptide fish protein flushing water-soluble fertilizer and a preparation method thereof, the water-soluble fertilizer is prepared from the following raw materials: unrotten small fish or fish sewer, edible cane sugar or brown granulated sugar, a self-prepared fish protein enzymolysis agent, a self-prepared fish protein anaerobic fermentation inoculant and water; the preparation method comprises an enzymolysis stage and a fermentation stage. The compound fertilizer can be used as a nitrogen fertilizer to replace a traditional chemical nitrogen fertilizer, or is matched with a trace element fertilizer in a compound fertilizer to be used in crop planting; the water-soluble fertilizer has the beneficial effects that the water-soluble fertilizer can supplement soil protein amino acid fertilizer nutrition and increase the content of nutritional ingredients in crops, fermented polypeptide micromolecule sugar can be directly absorbed by the crops to improve the taste, ecological organic planting is realized, meanwhile, the planting cost is reduced, and the water-soluble fertilizer is suitable for fruit and vegetable planting families, farms, picking gardens, greenhouse farmers and the like, is simple and easy to operate, and is suitable for popularization and application. Raw materials are easy to collect, low in cost and obvious in effect.

The invention discloses a sphingomonas sphingomonas, a bacterial agent thereof and application of the sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas The sphingomonas disclosed by the invention has excellent saline-alkaline tolerance, has the functions of fixing nitrogen, decomposing organic phosphorus and producing ACC deaminase, breaks through the limitation of single function of a traditional strain, and has a very good application prospect in saline-alkaline soil improvement, plant growth promotion and ecological restoration.

The invention relates to a lake sediment reduction bacterium which is classified and named as bacillus amyloliquefaciens WP02, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2020696, and the lake sediment reduction bacterium is preserved in the China Center for Type Culture Collection. The invention relates to an application of a lake sediment reduction bacterium in sediment denitrification, phosphorus uptake and quality degradation. The method has the beneficial effects that the immobilized microbial agent obtained by respectively embedding and adsorbing lake sediment reduction bacteria can effectively reduce sediment organic matters, reduce sediment total nitrogen and absorb phosphorus in overlying water, namely effectively perform denitrification, phosphorus absorption and quality degradation on the sediment, and the microbial agent can improve the oxidation reduction potential of the sediment and reduce the pollution of the sediment. And the method has important significance and application value in the aspect of maintaining the ecological balance of the water body, and has the characteristics of high in-situ remediation efficiency, small environmental disturbance and no secondary pollution compared with a physical and chemical remediation method.

The invention belongs to the technical field of microorganisms, and particularly relates to a Prussia adamsii T54 strain, a microbial agent and application of the Prussia adamsii T54 strain and the microbial agent. The Pichia aryabhattai T54 is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on February 21, 2025, and the preservation number of the Pichia aryabhattai T54 is CGMCC (China General Microbiological Culture Collection Center) No. 33613. According to the present invention, the Princisteria aryabhattai T54 and the bacterial agent have significant inhibition effects on a variety of plant pathogenic fungi, and further have plant growth promoting effects;

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus agave, fermentation liquor, a biological agent and application. The invention discloses a strain of bacillus tequilensis, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus tequilensis is CGMCC NO.34830. The strain of bacillus tequilensis has the advantages that the preservation number is CGMCC NO.34830; the invention also discloses a fermentation broth and a biological agent prepared according to the bacillus agave, and the bacillus agave, the fermentation broth and the biological agent provided by the invention can effectively reduce the harm of root knot nematode disease to plants and promote seed germination or crop growth, and have the advantages of high efficiency, safety, environmental protection and difficult generation of resistance.

The invention relates to the technical field of agricultural microorganisms. The invention provides a compound microorganism bacterium suspension, a bacterium agent, a tobacco seed coating agent as well as a preparation method and application of the compound microorganism bacterium suspension. The compound microorganism bacterium suspension comprises a rice seed endophytic bacterium spiraceae R3 bacterium suspension and a rice seed endophytic bacterium stenotrophomonas R5 bacterium suspension; the mass ratio of R3 to R5 is 1: (0.5-1.5); the endophytic bacterium herbapiril R3 of the rice seeds is Herbapiril sp. R3, and the preservation number of the herbapiril R3 is CCTCC (China Center For Type Culture Collection) NO: M 20231757; the rice seed endophytic bacterium stenotrophomonas R5 is Shenotrophomonas sp. R5, and the preservation number of the rice seed endophytic bacterium stenotrophomonas R5 is CCTCC (China Center for Type Culture Collection) NO: M 20231758. The compound microbial suspension is used as a seed coating agent to coat tobacco seeds, and growth of tobacco root systems can be remarkably promoted.

The invention relates to the technical field of aquaculture, and particularly discloses paracoccus pantotrophus P.p-O8 and application of the paracoccus pantotrophus P.p-O8, the paracoccus pantotrophus P.p-O8 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the paracoccus pantotrophus P.p-O8 is CGMCC NO.26522. The invention relates to a microbial inoculum prepared from paracoccus pantotrophus P.p-O8. The method has a huge application prospect in improving the quality of aquaculture water and repairing the polluted sediments in the cage culture area, improves the water environment quality and the survival conditions of aquaculture organisms, repairs the eutrophicated polluted ecological environment, and has high environmental protection value and economic benefit.

The invention discloses a multi-element soil fertility improvement biological modifier and a preparation method thereof, and belongs to the technical field of soil fertility improvement. The modifier is prepared from modified straw aerogel, a strontium titanate loaded lignin compound, lanthanide modified potassium feldspar powder, alginic acid oligosaccharide composite gamma-aminobutyric acid, carbon nitride quantum dots, bacterial cellulose nanofibers and polyaniline nanotubes according to the mass ratio of (18-22): (15-17): (13-15): (7-10): (10-13): (3-5): (7-10): (10-13): (5-8). The composite microbial inoculant is composed of a nano zero-valent iron-montmorillonite compound and a nitrogen fixation-phosphate solubilizing composite microbial inoculant. The modified straw aerogel and the bacterial cellulose nanofibers are interwoven to form a three-dimensional network framework, a habitat is provided for soil microorganisms, the water and fertilizer retention capacity of soil is enhanced, and meanwhile, the network structure promotes transmission of nutrients and water in the soil and improves the nutrient utilization efficiency.

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention belongs to the technical field of salt-tolerant growth-promoting bacteria, and particularly relates to a strain of Priestia aryabhattai PYJ2-8, a microbial inoculum, a preparation method and application of the strain of Priestia aryabhattai PYJ2-8. The invention provides a strain of P.adami PYJ2-8, and the preservation number of the P.adami PYJ2-8 is CGMCC (China General Microbiological Culture Collection Center) No.33769. According to the present invention, the Pristeria aryabhattai PYJ2-8 is the salt-tolerant growth promoting bacterium, can produce IAA, can decompose insoluble phosphorus, can improve the concentration of soluble phosphorus, and can increase the content of available phosphorus in soil; the utilization rate of the fertilizer can be increased, the growth and development and root development of plants can be promoted, and meanwhile, the potential capability of improving saline-alkali soil is also achieved.

The present application belongs to the technical field of biological fermentation, and discloses a natural plant fermentation liquor, which is prepared from acanthopanax, angelica sinensis, rosa davurica, phellinus baumii, tea leaves, water, mixed enzymes and mixed bacterial agents; wherein the mixed enzymes include cellulase, pectinase and tannase, and the mixed bacterial agents include lactobacillus plantarum, schizosaccharomyces and lactobacillus acidophilus.The natural plant fermentation liquor has high safety, good clarity after adjusting the concentration of crude drugs, and can be added into a cosmetic matrix.The preparation method is simple, and the effective active ingredients of the natural plants and tea leaves are extracted by the synergistic effect of bacteria and enzymes to the maximum extent.In addition, the present application also discloses the application of the natural plant fermentation liquor in preparing cosmetics for treating oily sensitive skin.

The present invention belongs to the technical field of biological treatment of environmental pollutants, and specifically relates to a strain of low-temperature-resistant multifunctional bacteria and its application in soil and water pollution remediation. The low-temperature-resistant multifunctional bacteria is the strain TLT1 of the genus Tularemia, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on August 9, 2023, with the deposit number: CGMCC No: 40754. The bacterial agent of the present invention can be used to stabilize zinc in the soil environment, which helps to enrich the database of bacterial strains that stabilize heavy metals and have the ability to degrade quinolone antibiotics. The present invention prepares the purified strain into a bacterial suspension, which can effectively stabilize zinc in different environmental media such as water and soil under low-temperature conditions, and has the ability to degrade enrofloxacin in different environmental media such as water and soil. Compared with methods such as chemical oxidation, this method is more efficient, less expensive, and has no secondary pollution, providing an effective biological-non-biological combined remediation technology for the remediation of zinc-enrofloxacin complex-contaminated soil and water.

The invention discloses bacillus orientalis ZL3 for regulating rhizosphere soil microecosystems of haloxylon ammodendron soil in deserts and promoting the growth of the haloxylon ammodendron soil, particularly relates to a fungicide ZL3 for promoting the growth of haloxylon ammodendron and regulating rhizosphere microbial communities and application of the fungicide ZL3, and belongs to the technical field of microorganisms. According to the bacillus toonensis ZL3 provided by the invention, the conversion of soil nutrients to available nutrients is improved, and the rhizosphere microbial community structure is improved, so that the effect of promoting the growth of haloxylon ammodendron is achieved. When the ZL3 is applied to a haloxylon ammodendron field, the content of soil organic matters, the content of soil quick-acting potassium, the content of available phosphorus and the activity of sucrase are remarkably improved by 37.78%, 11.01%, 49.66% and 67.71% respectively. The plant height is increased by 17.59%, and the root length is increased by 109.45%. Meanwhile, the positive change of microbial communities is regulated and controlled, especially the enrichment of beneficial bacteria, the relative abundance of the prevotella and the vibrio is remarkably increased, and the relative abundance of the burkholderia is remarkably reduced. In general, inoculation of the ZL3 fungicide can promote the growth and development of haloxylon ammodendron by improving the soil fertility and improving the rhizosphere microflora structure.

The invention discloses a compound bacterial agent for reducing blood sugar and blood fat, application of the compound bacterial agent and a method for preparing a fermented coconut milk beverage from the compound bacterial agent, belongs to the technical field of microorganisms, and particularly relates to the compound bacterial agent for reducing blood sugar and blood fat, the compound bacterial agent comprises fermented lactobacillus mucus AL-8 and lactobacillus paracasei DX-5, and the compound bacterial agent is prepared from the fermented lactobacillus mucus AL-8, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5. The preservation number of the lactobacillus mucus AL-8 is CCTCC (China Center For Type Culture Collection) NO: M20252735; the preservation number of the lactobacillus paracasei DX-5 is CCTCC (China Center For Type Culture Collection) NO: M20252736. According to the invention, the fermented coconut milk beverage is prepared by utilizing the inhibition effect of a blood sugar and blood fat reducing compound bacterial agent consisting of fermented lactobacillus mucus AL-8 and casei paracasei DX-5 on alpha-amylase and pancreatic lipase.

The invention relates to the technical field of microorganisms, and discloses Ackermann muciniphila, a combined microbial agent thereof and application of the Ackermann muciniphila in inflammatory enteritis. The invention provides Akkermansia muciniphila AIML-559, and the preservation number of the Akkermansia muciniphila AIML-559 is CGMCC (China General Microbiological Culture Collection Center) No. 34246. The invention relates to a combined microbial agent, which is characterized by comprising Ackermann muciniphila AIML-559 and Bifidobacterium adolescentis AIML-528 (the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.34300). The combined microbial agent is prepared from the following raw materials: Ackermann muciniphila AIML-559 and Bifidobacterium adolescentis AIML-528 The ackermann mucophil combined microbial inoculum is suitable for treatment intervention of inflammatory bowel disease (IBD), and can relieve weight loss, hemolysis, anemia, diarrhea, intestinal bleeding and other symptoms of IBD mice.

The utility model discloses an isolation belt device for blocking and treating acid mine wastewater, and belongs to the technical field of mine wastewater isolation. The device comprises a buoy, an isolation belt fixing frame is arranged at the bottom of the buoy, a weight settling box is arranged at the bottom of the isolation belt fixing frame, and filler is filled in the weight settling box; biomass filler is arranged in the isolation belt fixing frame, and three-dimensional elastic brush filler is arranged on the front side and the rear side of the isolation belt fixing frame in a binding mode. The device disclosed by the utility model is simple in structure, can be effectively assembled according to site conditions, can be in contact with the bottom, realizes multi-depth treatment, can effectively reduce the concentration of dissolved oxygen converged into AMD, gradually improve the bottom layer environment, promote the growth of anaerobic microorganisms, perform biofilm formation synergistic treatment by using indigenous microorganisms and avoid the problem of adaptability of exogenous microbial agents, and can be effective for a long time; the cost and the risk of additionally adding nutrient sources and microorganisms are reduced.

The invention relates to the technical field of microorganisms, in particular to a strain of Senotrophomonas sepilia DB3 with disease-resistant, iron-dissolving and growth-promoting functions and application thereof, and the strain is separated from calcareous soil pear tree rhizosphere and has good capabilities of producing IAA (Indoleacetic Acid), quickly growing, dissolving insoluble iron, producing iron carriers and effectively antagonizing various pathogenic bacteria. Pot experiments show that the DB3 microbial inoculum can significantly promote growth of peanut and pyrus betulaefolia seedlings, improve root development and iron nutrient content, enhance leaf SPAD value and iron absorption and relieve iron-deficiency yellows, can be used for preparing biological bacterial fertilizers and reducing chemical iron fertilizer investment, and has good development and application prospects.

The present disclosure belongs to the technical field of microorganisms, and in particular relates to a nano-coated growth-promoting bacterial agent and a composite growth-promoting bacterial agent, preparation methods and use thereof. The nano-coated growth-promoting bacterial agent includes a growth-promoting bacterial agent and a nano-coating formed by self-assembly of a plant-derived polyphenol and a soluble multivalent metal ion on the surface of the growth-promoting bacterial agent. The key to the present disclosure is to employ the plant-derived polyphenol and the soluble multivalent metal ion to self-assemble on the surface of the growth-promoting bacterial agent to form a nano-coating. Based on the interaction of the growth-promoting bacterial agent with the phyllosphere, the leaf face adhesion performance of the growth-promoting bacterial agent is significantly enhanced, the loss rate of the growth-promoting bacterial agent under rainfall erosion is effectively reduced, and the survival rate and colonization of the growth-promoting bacterial agent in the phyllosphere are enhanced.

The invention discloses a potato high-yield disease-resistant controlled-release special fertilizer and application thereof, and relates to the technical field of fertilizer production. The high-yield disease-resistant controlled-release fertilizer special for the potatoes is prepared by adopting synergistic controlled-release urea coated with double-layer microbial agents and a controlled-release potassium-magnesium fertilizer as raw materials, wherein the synergistic controlled-release urea coated with the double-layer microbial agents is prepared by performing double-layer coating on urea through a micro-capsule loaded microbial agent. The fertilizer prepared by the invention has an optimized nutrient proportion of high potassium, medium and high nitrogen and low phosphorus, and the nutrient type and dosage accord with the nutrient requirement rule of the potatoes on macroelements. Meanwhile, the controlled release effect is realized by adopting a modified sulfur coating technology, so that the nutrient requirements of the potatoes in the key growth period are met. Through the comprehensive nutrient controlled release and inner and outer film layer fungicide loading technology, the nutrient utilization efficiency can be greatly improved, the potassium and magnesium absorption rate and the plant resistance of the potatoes are improved, diseases are reduced, the yield of the potatoes per unit area is remarkably improved, and the fertilization amount and the fertilization frequency can be reduced.

The invention belongs to the field of biological medicine, and particularly relates to bifidobacterium bifidum and application of the bifidobacterium bifidum in eye health products, and the bifidobacterium bifidum BB00 has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.34389. The invention further discloses a preparation method of the bifidobacterium bifidum. The bifidobacterium bifidum BB00 can improve clinical symptoms of xerophthalmia, increase tear secretion amount, prolong tear film rupture time, reduce ocular surface disease indexes, relieve corneal epithelium injury, adjust the opening degree of palpebral fissure, adjust intestinal flora composition, improve flora diversity and inhibit growth of intestinal pathogenic bacteria. The bifidobacterium bifidum BB00 not only provides an innovative solution for a clinical pain point of xerophthalmia and realizes synergistic intervention of intestinal regulation and eye health, but also has scientific innovation, clinical effectiveness and industrialization potential, and has important value for application of bacteria and fungicide in the field of biological medicine and treatment of ocular surface-intestinal tract related diseases.

The invention belongs to the technical field of microbial fermentation and biological agriculture, and discloses a soil remediation fungicide as well as a preparation method and application thereof. The preparation method comprises the following steps: activating bacillus megatherium Z101, inoculating the activated bacillus megatherium Z101 into fermentation liquor containing rice straw powder, carrying out fermentation culture, and carrying out vacuum spray drying on fermentation mixed liquor after fermentation is completed, so as to obtain soil remediation bacterium powder; or further mixing the soil remediation bacterium powder with auxiliary materials and water, and granulating to obtain the soil remediation bacterium granules. The application method comprises the following steps: in a rice planting stage, applying the soil remediation fungicide and an inorganic fertilizer together to remedy rice field soil and promote rice growth. The soil remediation fungicide prepared by fermentation culture of the bacillus megatherium Z101 has remarkable advantages in the aspects of rice yield increase, soil pH value increase and physicochemical property improvement, and a new application way is provided for the bacillus megatherium Z101.

The invention belongs to the technical field of food microorganisms, and particularly relates to an enterococcus lactis NSK220 strain, a microbial agent and application of the enterococcus lactis NSK220 strain. The invention provides the enterococcus faecium NSK220, the clearance rate of the enterococcus faecium NSK220 on DPPH is very high, the serum SOD enzyme activity can be improved by supplementing the strain NSK220 in diet, and it is indicated that the oxidation resistance of the strain NSK220 is high. Meanwhile, the enterococcus faecium NSK220 has relatively strong self-cohesion and surface hydrophobicity, which indicates that the strain has strong colonization ability and is beneficial to long-term play of probiotic functions in intestinal tracts; the strain NSK220 can also improve the feed intake and kidney function of animals, and is beneficial to the growth and body health of the animals. The enterococcus faecium NSK220 has an inhibition effect on escherichia coli, staphylococcus aureus, salmonella, pseudomonas aeruginosa and listeria monocytogenes.

The invention belongs to the technical field of agricultural fertilizers, and particularly relates to an amino acid water-soluble fertilizer containing chitosan oligosaccharide and salt-tolerant bacillus and a preparation method of the amino acid water-soluble fertilizer. According to the method, litopenaeus vannamei heads are used as raw materials, a refined solution is prepared through mixed acid pretreatment, double-enzyme fermentation and nanofiltration membrane desalination, and the refined solution is mixed with a salt-tolerant domesticated bacillus licheniformis / bacillus natto solution, citric acid-malic acid chelated medium elements and a protective agent. According to the method, by optimizing a pretreatment process and a chelating system, synergistic degradation of shrimp head protein and chitin is achieved, the prepared fertilizer has the compound functions of amino acid, chitosan oligosaccharide and a salt-tolerant microbial agent, the problems that a microbial agent in a traditional fertilizer is poor in salt tolerance and prone to precipitation are solved, and the method is suitable for facility agriculture and saline-alkali soil and has a wide application prospect. The crop stress resistance can be enhanced; the growth effect is improved.

The invention belongs to the technical field of microorganisms, and particularly discloses bacillus impetus DXGZ2-6 as well as a microbial agent and application thereof. The preservation number of the bacillus haynesii DXGZ2-6 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.33710. The bacillus haynesii DXGZ2-6 disclosed by the invention has the advantages that the preservation number is CGMCC No.33710 The bacillus marinensis can generate various enzymes, can effectively promote plant growth, slows down decomposition or transfer of chlorophyll of plant leaves, and can effectively inhibit various phytopathogens.

The invention discloses a tea leaf theanine composition for protecting a central nervous system as well as a preparation method and application thereof, relates to the field of common foods and health-care foods, and aims to solve the problems that most of existing central nervous protection products are single components or are simply compounded, the synergistic effect is weak and the bioavailability is low. The preparation method comprises the following steps: S1, mixing the tea leaf theanine, the emblic leafflower fruit powder, the ginkgo leaf extract, the wolfberry fruit powder and other raw materials according to a specific ratio, and performing high-temperature sterilization and cooling to obtain a fermentation substrate; s2, inoculating a composite activation microbial inoculum composed of plant lactobacillus, lactobacillus acidophilus and lactobacillus reuteri, and carrying out constant-temperature anaerobic fermentation; and S3, freezing and centrifuging at 4 DEG C, concentrating with an RO membrane, and freezing and drying to obtain the composition. The viable count of the complex microbial inoculant reaches 2.0 * 10 < 9 >-3.0 * 10 < 9 > CFU / mL, and the strain ratio and the fermentation process are optimized. The composition has a synergistic effect of multiple components, can efficiently protect nervous centralis, and is suitable for preparing related common food, health food or functional food.

The invention discloses bacillus velezensis FSUXF-717 capable of promoting diastatic fermentation and improving flavor of fermented wine and application of the bacillus velezensis FSUXF-717, and relates to the technical field of microorganisms. The preservation number of the bacillus velezensis FSUXF-717 provided by the invention is GDMCC No: 64133, the fermentation liquor and microbial inoculum of the strain can promote the saccharification process of the wine, and meanwhile, the strain can enhance the fermentation to significantly increase the total amino acid content (plt: 0.05) and increase the variety and content of flavor substances such as esters and organic acids, and has a promoting effect on the flavor formation of the wine body. The acquisition of the strain provides a strain resource for the development of wine brewing and fermented food, has important practical significance for promoting high-efficiency and high-quality wine brewing, and also provides a theoretical basis and technical support for the directional regulation and control of the quality of traditional fermented food.

The invention discloses a method for preparing live pig feed through mixed solid-state fermentation of feed rape and rapeseed meal. The method specifically comprises the following steps: harvesting feed rape from a full-bloom stage to a final bloom stage, adding rapeseed dregs, and uniformly mixing to obtain a fermentation substrate with the water content of 55-65%; spraying the compound enzyme bacterium preparation on the fermentation substrate, uniformly stirring, finally compacting by using a packer, sealing, packing and storing, and fermenting for 45 days; the compound enzyme bacterial preparation comprises a bacterial agent and an enzyme agent; the fungicide comprises lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and aspergillus niger; the enzyme agent is prepared from phytase, cellulase, hemicellulase, pectinase, tannase, xylanase and alpha-galactosidase. The feed rape and the rapeseed meal are subjected to mixed solid fermentation, so that the content of phytic acid, glucosinolate and crude fiber is greatly reduced, the content of crude protein is increased, and the nutritional quality of the feed is remarkably improved. The fermented feed is used for replacing part of corn, soybean meal and wheat bran, and the use amount of concentrated feed in live pig breeding is reduced.