62 results about "Pseudomonas fluorescens" patented technology

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for heavy metal contaminated soil and a biological bacterial fertilizer and a preparation method thereof. The micro-ecological restoration liquid complex microbial inoculant suitable for the heavy metal contaminated soil comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azotobacter braziliensis CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and restoration bacteria: pseudomonas putida CGMCC 1.4533. The biological bacterial fertilizer for the heavy metal contaminated soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the heavy metal contaminated soil is greatly improved, the heavy metal contaminated soil is effectively improved, and the crop yield is remarkably increased.

The invention belongs to the technical field of biological phosphorus-silicon fertilizers, and particularly relates to a liquid complex microbial inoculant capable of repairing heavy metals and activating phosphorus and silicon and a biological phosphorus-silicon fertilizer thereof. The liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria Cupriavidus and Ochrobactrum, nitrogen-fixing bacteria Azospirillum brasilense, phosphate solubilizing bacteria Bacillus megaterium and Pseudomonas, potassium solubilizing bacteria Bacillus mucilaginosus, growth-promoting bacteria Pseudomonas fluorescens, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants and microbial inoculants. The biocontrol bacteria are trichoderma harzianum and bacillus subtilis, and the synergistic bacteria are saccharomyces cerevisiae; the biological phosphorus-silicon fertilizer is prepared from the liquid complex microbial inoculant. According to the biological phosphorus-silicon fertilizer provided by the invention, through scientific compatibility of various functional strains, heavy metal remediation and soil phosphorus and silicon solubilization are realized, absorption of phosphorus and silicon in soil is promoted, and the crop yield is increased.

This invention belongs to the field of bio-mineral fertilizer technology, specifically relating to a microbial liquid compound agent and bio-mineral fertilizer that combine coal gangue activation and heavy metal remediation. The microbial liquid compound agent comprises the following functional strains prepared through three-stage sequential inoculation and fermentation using electrochemical low-Hertz water: *Pseudomonas putida* (a remedial bacterium), *Azotobacter chrysogenum* (a nitrogen-fixing bacterium), *Azospirillum brasiliensis* (a growth-promoting bacterium), *Pseudomonas fluorescens* (a phosphate-solubilizing bacterium), *Bacillus subtilis* (a biocontrol bacterium), *Bacillus mucilaginosus* (a potassium-solubilizing bacterium), *Bacillus megaterium* (a silica-solubilizing bacterium), and *Trichoderma echinococcus* (a synergistic bacterium). The bio-mineral fertilizer is prepared using the aforementioned microbial liquid compound agent, with coal gangue powder as the main raw material. The scientific combination of different strains provided by this invention achieves efficient improvement of heavy metal-contaminated land and increased crop yields. Simultaneously, it transforms coal gangue into a valuable resource, solving the pollution problem caused by the large-scale accumulation of coal gangue, and possesses significant economic and social value.

The invention relates to the technical field of biology, in particular to pseudomonas fluorescens GS-1 for synthesizing polyhydroxyalkanoate and application of the pseudomonas fluorescens GS-1, the pseudomonas fluorescens GS-1 is preserved in China General Microbiological Culture Collection Center on February 6, 2026, and the preservation number is CGMCC No.37739; the strain carries a phaC gene and can synthesize PHA, and the straw sugar PHA conversion efficiency reaches 87.7% of that of a glucose system; the method has inherent lignocellulose hydrolysate inhibitor tolerance, and the PHA synthesis capability retention rate in the complex self-made corn straw saccharification liquid reaches 91.4%. A high-quality strain resource with direct application potential is provided for producing the PHA based on cheap straw saccharification liquid.

The invention relates to an artificial phosphate-solubilizing module and application thereof in improving the nitrogen-fixing and phosphate-solubilizing capabilities of nitrogen-fixing microorganisms. The artificial phosphate solubilizing module sequentially comprises an inducible promoter element and a conservative acid phosphatase gene acpA from a pseudomonas fluorescens strain from upstream to downstream. The artificial phosphate solubilizing module is inserted into a preset target site of a host microorganism chromosome in a homologous recombination manner, so that a host has the characteristic of efficiently expressing acid phosphatase, and the organic phosphorus degradation capacity and nitrogen fixation capacity of the host are remarkably improved. The method has high application value in the field of preparation of high-efficiency microbial fertilizer products. The method opens up a new path for agricultural green sustainable development, and has extremely important scientific significance and wide application prospects.

The invention belongs to the technical field of biological bacterial fertilizers, and particularly relates to a high-salt-tolerance microbial liquid complex microbial inoculant suitable for saline-alkali desertification land and a biological bacterial fertilizer thereof. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation of three stages: salt-tolerant repair bacteria Nocardia halophila, salt-tolerant functional bacteria Bacillus subtilis and Azospirillum brasilense, nitrogen-fixing functional bacteria Azotobacter chroococcum, phosphorus-solubilizing functional bacteria Pseudomonas fluorescens, and nitrogen-solubilizing functional bacteria Pseudomonas fluorescens. The bacillus mucilaginosus is used as a potassium-dissolving functional bacterium, the trichoderma asperellum is used as a biocontrol growth-promoting bacterium, and the trichoderma harzianum is used as a rhizosphere optimizing bacterium. The biological bacterial fertilizer is prepared from the liquid complex microbial inoculant. Through scientific compatibility of different strains provided by the invention, efficient improvement and crop yield increase of the saline-alkali desertification land are realized, and the strain has important economic and social values.

The invention discloses pseudomonas fluorescens with benzo [a] anthracene degradation capacity and application of the pseudomonas fluorescens, and relates to the technical field of microbial degradation. The bacterial strain is Pseudomonas fluorescens SXU-ZW-01 and is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, the preservation number of the bacterial strain is CGMCC NO.36511, and the preservation date of the bacterial strain is November 05, 2025. The strain is separated from an environmental sample of a coking plant, has a typical high-temperature adaptation characteristic and can efficiently degrade benzo [a] anthracene under the conditions that the temperature is 45 DEG C and the pH value is 7.0-8.0, and the degradation rate of benzo [a] anthracene within 96 hours can reach 95% or above. The invention provides core strain resources and technical support for green treatment of coking flue gas, and has good industrial application prospects.

The invention relates to the technical field of seed coating, in particular to a coating agent and a preparation method thereof. The coating agent comprises compound microorganisms, an antibacterial enhancer, a film-forming agent and a dispersing agent; the compound microorganisms comprise azospirillum, pseudomonas fluorescens, bacillus mucilaginosus and trichoderma harzianum; the antibacterial reinforcing agent is prepared from chitosan and epsilon-polylysine; the mass ratio of the compound microorganisms to the antibacterial enhancer is (1-3): (0.5-1). According to the coating agent and the preparation method thereof provided by the invention, the double effects of seed storage and preservation and growth promotion can be taken into account, and meanwhile, the environmental adaptability can be further improved.

The invention discloses a targeted biological bacterium coupling method for comprehensive treatment of coastal vegetable salt damage, heavy metal and pesticide residues and application, and relates to the field of agricultural biotechnology and soil remediation. According to the method, bacillus subtilis, bacillus mucilaginosus, brevibacillus laterosporus, pseudomonas fluorescens, arbuscular mycorrhizal fungi and host original flora extracted from healthy vegetable rhizosphere soil are subjected to coupled symbiotic culture, and target flora with multiple functions is formed. The functional flora is compounded with a nutrient carrier containing potassium humate and fulvic acid, a seaweed extract and a specific salt-resistant additive to prepare the multifunctional water-soluble fertilizer or fungicide. After the compound fertilizer is applied to a coastal vegetable production base, the synergistic effects of degrading soil pesticide residues, passivating heavy metals, enhancing the salt tolerance of vegetables and improving the soil fertility and microbial diversity can be synchronously realized, the compound obstacle problem of coastal vegetable production is fundamentally solved, and the yield, quality and safety of vegetables are guaranteed.

The present application relates to the technical field of calcium carbonate manufacturing, and particularly relates to a preparation method of high-whiteness light calcium carbonate. The method comprises the following steps: efficient leaching of calcium element in carbide slag; preparation of microbial crystal form control agent; and electric dispersion treatment and precipitation of calcium ions. The microbial crystal form control agent is prepared by adjusting the mixing ratio of Bacillus pumilus fermentation liquor and Pseudomonas fluorescens fermentation liquor, so that Ca 2+ The stearic acid and sodium polyacrylate in the microbial crystal form control agent can make the particles more stable and provide ion adsorption capacity after the larger calcium carbonate particles are mineralized, CO2 is introduced after temperature rising, and the crystal form of the calcium carbonate is optimized, so that the vaterite type crystallization with smaller crystal form is formed. The Bacillus pumilus can also assist in generating small-particle-size calcium carbonate over time, so that the high-whiteness small-particle-size light calcium carbonate is obtained.

The invention relates to the technical field of bioengineering, in particular to an ester hydrolase mutant and application thereof. According to the present invention, the screened wild-type ester hydrolase derived from Pseudomonas fluorescens is subjected to mutation to obtain the ester hydrolase mutant, the ester hydrolase mutant is applied to the synthesis of the sitagliptin intermediate, and compared with the wild-type ester hydrolase, the ester hydrolase mutant has characteristics of high enzyme activity and high stereoselectivity on the substrate (compound II), the enzyme consumption is significantly reduced, and the yield of the sitagliptin intermediate is significantly improved; the chiral purity of the reaction product (compound I) is greatly improved, and the method has a good industrial application prospect.

The present application relates to the field of bio-agriculture, and particularly relates to a bio-agricultural compound microbial fertilizer and a preparation method thereof, which comprises, by weight, 2-8 parts of functional microbial flora, 20-48 parts of organic carrier, 5-20 parts of nutrient component, 0.5-4 parts of microbial body protective agent, and the balance of water; the functional microbial flora is composed of Pseudomonas fluorescens, Trichoderma harzianum, Saccharomyces cerevisiae and Bacillus mycoides; and the organic carrier is composed of a peanut shell-sugarcane residue mixture, modified diatomite and humic acid. The present application forms a complete compound microbial fertilizer technical solution through scientific compounding of multiple strains, directional modification of the organic carrier, balanced optimization of the nutrient component and low-temperature preparation process synergy, and has multiple outstanding beneficial effects.

The invention relates to the technical field of plant cultivation, in particular to an edible mushroom cultivation material and a preparation method and application thereof. The invention provides an edible mushroom cultivation material. The edible mushroom cultivation material comprises 10-25 parts of corncob, 10-20 parts of rape straw, 5-8 parts of soybean meal, 1-3 parts of leonurine, 1-2 parts of naringenin, 1-2 parts of sugar and 1-3 parts of a complex microbial inoculant, the complex microbial inoculants comprise candida utilis, lactobacillus plantarum, trichoderma harzianum, pseudomonas fluorescens and pediococcus pentosaceus. According to the edible mushroom cultivation material provided by the invention, each component has different effects and can generate a synergistic effect at the same time, so that the yield of edible mushrooms is increased to the maximum extent, the contamination rate of infectious microbes can be reduced, and a new direction is provided for edible mushroom cultivation.

The invention discloses a nutrition regulation and control method for disease resistance of kiwi fruits, a germination fertilizer and a preparation method, and the preparation method comprises the following steps: constructing a ternary interaction system consisting of a sulfur source, succinic acid and Pseudomonas fluorescens, and applying the ternary interaction system to rhizosphere nutrition management of the kiwi fruits in early spring. And three-dimensional coupling of nutrition supply, signal activation and microbial regulation and control is synchronously realized in the rhizosphere microdomain. Meanwhile, lignin monomer synthesis is continuously activated and promoted through a salicylic acid signal channel, high-activity laccase serves as a key enzyme for lignin monomer polymerization, lignin monomer polymerization reaction is accelerated, synthesis and deposition of lignin in plant cell walls are promoted, plant endogenous disease-resistant signal cascade is continuously amplified, and plant disease resistance is improved. The kiwi fruits are induced to generate stable systematic disease resistance. The composition can prevent and control diseases for a long time and cooperatively promote plant growth.

The invention belongs to the technical field of microorganisms, and particularly relates to a complex microbial inoculant for relieving phosphorus deficiency in the seedling stage of corn covered with straw and application of the complex microbial inoculant. Bacillus subtilis, saccharomycetes and pseudomonas fluorescens are compounded into the complex microbial inoculant, saccharomycetes secrete IAA and siderophores to promote the root development of seedlings, through domestication culture, the adaptability of the microbial inoculum in a straw-covered low-temperature environment is enhanced, the activation capacity on phosphorus is improved, the phosphorus nutrition level is synergistically improved in cooperation with bacillus subtilis, pseudomonas fluorescens can enhance the resistance of the seedlings to low-temperature stress, and the survival rate of the seedlings is increased. Soil-borne pathogenic bacteria which cannot be effectively inhibited by some soil florae are specifically inhibited, the influence of harmful bacteria on the activation effect of phosphorus is reduced, the three strains are matched to remarkably improve the content of rapidly available phosphorus in soil, reduce the incidence rate of phosphorus deficiency symptoms and improve the stress resistance and growth indexes in the corn seedling stage, and the compound microbial agent is suitable for a straw covering cultivation mode.

The invention discloses a compound microbial agent for reducing high-damp-heat morbidity of muskmelons and application of the compound microbial agent, and belongs to the technical field of agricultural cultivation. The active ingredients of the compound microbial agent comprise bacillus subtilis, pseudomonas fluorescens, trichoderma harzianum and saccharomyces cerevisiae. According to the compound microbial preparation, under the synergistic effect of four microorganisms, the comprehensive prevention and control effect on multiple diseases of muskmelons is remarkably improved, and the core problem that the diseases of the muskmelons frequently occur in the high-humidity-heat environment is effectively solved. Meanwhile, compared with chemical pesticides, the compound microbial agent provided by the invention has no residual risk, meets the development requirements of green agriculture, and avoids drug resistance and potential food safety hazards.

The invention relates to the technical field of agricultural microbial fertilizers, in particular to a preparation method of a high-activity multifunctional foliar bacterial fertilizer, which comprises the following steps: preparing a composite culture medium, and accurately calculating the mass ratio of fish peptone to trehalose based on a target carbon nitrogen ratio; inoculating bacillus atrophaeus, bacillus siamensis and pseudomonas fluorescens in a specific proportion into the culture medium, and performing dynamic fed-batch fermentation; after fermentation, thalli are wrapped by forming protective colloid, and the addition condition of the microelement mother liquor is strictly controlled to protect the activity of the thalli; and finally, based on the feedback of the survival rate of the finished product thalli, intelligently adjusting the technological parameters of the next batch. The prepared foliar bacterial fertilizer has the advantages of being high in thallus activity, comprehensive in function, good in storage stability and the like, and upgrading from a fixed formula to self-adaptive process optimization is achieved.

This invention relates to the field of bioengineering technology, and more particularly to the application of *Pseudomonas fluorescens* in the control of southern root-knot nematode disease. The application includes: the use of *Pseudomonas fluorescens*, or a bacterial agent containing *Pseudomonas fluorescens*, or a siderophore secreted by *Pseudomonas fluorescens* in the control of southern root-knot nematode disease; the *Pseudomonas fluorescens* has the preservation number CGMCC No. 16925. This invention has found that *Pseudomonas fluorescens* with preservation number CGMCC No. 16925 can secrete a siderophore that can effectively inhibit the infection and reproduction of southern root-knot nematode disease, reducing the number of root-parasitic nematodes by approximately 46% and the root-knot index by approximately 37.5%, thereby effectively controlling southern root-knot nematode disease and improving crop quality. The *Pseudomonas fluorescens* and its secreted siderophore provided by this invention have significant application value in the integrated control of southern root-knot nematodes throughout their entire growth cycle.

This invention proposes a method for the biosynergistic extraction and refined utilization of all active ingredients in ginseng, belonging to the fields of medical, dental, or cosmetic formulations. This invention utilizes ginseng stress extract obtained through specific microbial treatment. This method leverages the stress stimulation of *Pseudomonas fluorescens* to efficiently synthesize various saponins, polysaccharides, and volatile oils in ginseng, resulting in an extract rich in components and with enhanced activity. Combining fractional extraction, resin purification, and nanoemulsification processes, a skincare raw material with comprehensive components, high stability, and excellent bioavailability is ultimately obtained, exhibiting a powerful synergistic effect in anti-inflammatory and anti-aging properties.

The application discloses a Rosa chinensis disease prevention and growth promotion bacterium LX6 strain and application thereof, and belongs to the field of microorganism strains and application thereof. The Rosa chinensis disease prevention and growth promotion bacterium LX6 is preserved in the China General Microbiological Culture Collection Center on May 16, 2024, and has a preservation number of CGMCC No. 30657 and a classification name of Pseudomonas fluorescens. The bacterium strain has the ability to resist high-concentration salt ions and various heavy metal ions. The bacterium strain can inhibit the growth of a Rosa chinensis black spot pathogen, i.e., Discosia rosea, and can also antagonize the growth of plant pathogenic fungi, i.e., Colletotrichum gloeosporioides, Rhizoctonia solani, Fusarium oxysporum and Fusarium equiseti, so as to prevent and treat the Rosa chinensis black spot and powdery mildew, and promote the growth and flowering of the Rosa chinensis. The application provides a fine biocontrol strain for preventing and treating the diseases of the Rosa chinensis and promoting the growth of the Rosa chinensis, and has important significance for green prevention and control technology of the diseases of the Rosa chinensis and sustainable development of the Rosa chinensis industry.

The invention relates to pseudomonas fluorescens JL12 with the effect of preventing and treating tobacco black shank and application of the pseudomonas fluorescens JL12. The pseudomonas fluorescens JL12 is classified and named as pseudomonas fluorescens, and the preservation number of the pseudomonas fluorescens JL12 is CGMCC No. 34713. The pseudomonas fluorescens JL12 strain can effectively antagonize phytophthora, prevent the incidence of tobacco black shank and weaken related symptoms of the tobacco black shank, so that the pseudomonas fluorescens JL12 strain can be used for preparing products for preventing and treating the tobacco black shank and improving the tobacco cultivation quality and yield.

This invention belongs to the field of aquaculture wastewater treatment technology, specifically relating to a quantitative addition of a multi-strain volcanic rock carrier composite, its preparation method, and its application. The floating volcanic rock of this invention can be stably suspended in the upper high-oxygen zone, suitable for aerobic nitrifying strains such as *Nitrobacterium velutipes* and *Nitrosomonas cerevisiae*; the suspended volcanic rock can remain in the middle medium-oxygen zone, carrying facultative strains such as *Pseudomonas fluorescens*; and the submerged volcanic rock can sink to the bottom low-oxygen / anaerobic zone, fixing denitrifying strains such as *Rhodopseudomonas palustris*. A vertical space of at least 2 meters in water depth is sufficient to construct a clear oxygen gradient, meeting the metabolic needs of different strains, thus achieving compatibility between the carrier and water depth and oxygen layer. This invention can precisely control the dissolved oxygen concentration in each layer, effectively avoiding oxygen layer homogenization caused by single aeration, helping to ensure the synergistic effect of aerobic, facultative, and anaerobic strains, and adapting to the growth needs of various functional bacterial communities.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for saline-alkali soil and a biological bacterial fertilizer of the micro-ecological restoration liquid composite microbial agent. The micro-ecological restoration liquid complex microbial inoculant suitable for the saline-alkali soil is prepared from restoration salt-tolerant bacteria, namely bacillus subtilis, nocardia halospora, nitrogen-fixing bacteria, namely azotobacter chroococcum, phosphate solubilizing bacteria, namely pseudomonas fluorescens, potassium solubilizing bacteria, namely bacillus mucilaginosus, growth-promoting bacteria, namely azospirillum brasilense, biocontrol bacteria, namely bacillus megatherium and synergistic bacteria, wherein the restoration salt-tolerant bacteria are bacillus subtilis, nocardia halospora, the nitrogen-fixing bacteria are azotobacter chroococcum, the phosphate solubilizing bacteria are pseudomonas fluorescens, the potassium solubilizing bacteria are bacillus mucilaginosus, the growth-promoting bacteria are azospirillum brasilense, the biocontrol bacteria are bacillus megatherium, and the synergistic bacteria are prepared from bacillus subtilis The biological bacterial fertilizer for the saline-alkali soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the saline-alkali soil is greatly improved, the saline-alkali soil is effectively improved, and the crop yield is remarkably increased.

The invention provides a preparation process of a zanthoxylum bungeanum root rot resistant microbial organic fertilizer. Relates to the technical field of agricultural microbial organic fertilizer preparation, and comprises the following steps: extracting strains from rhizosphere soil and diseased plant roots of zanthoxylum bungeanum maxim, screening microorganisms with antagonistic activity on zanthoxylum bungeanum maxim root rot pathogenic bacteria, and compounding the microorganisms into a composite strain according to a predetermined ratio. According to the preparation process of the zanthoxylum bungeanum root rot resistant microbial organic fertilizer, strains with high antagonistic activity, such as bacillus, pseudomonas fluorescens and trichoderma, are screened and compounded, so that a strong inhibition effect on zanthoxylum bungeanum root rot pathogenic bacteria is ensured, and the root rot resistant effect is improved. The ratio of the organic matrix to the auxiliary materials is optimized, the strain can better adapt to and proliferate after colonization according to the requirement of the pepper root system, and the problems that in the prior art, the survival rate is low, and colonization is difficult are solved. The temperature, the humidity, the ventilation capacity and the pH value are regulated and controlled through the intelligently controlled solid state fermentation device, it is ensured that strains proliferate in the optimal environment, and the number and the activity of thalli are improved.

The invention provides a functional microbial fertilizer for improving root rot resistance of crops and a preparation process thereof, and belongs to the technical field of microbial fertilizers. The bacterial fertilizer is prepared from 10-20 parts of a functional microorganism complex microbial inoculant, 30-50 parts of a modified carrier, 20-40 parts of a nutrition conditioner and 1-5 parts of a stabilizer, the complex microbial inoculant is prepared by compounding bacillus amyloliquefaciens J12, pseudomonas fluorescens Y8 and trichoderma harzianum Z3 according to the viable count ratio of 3: 2: 1; the modified carrier is a decomposed straw-humic acid-vermiculite composite carrier modified by chitosan hydrochloride. The preparation process adopts a step-by-step fermentation method to prepare the complex microbial inoculant, and combines the steps of modified carrier preparation and bacterial fertilizer composite aging. According to the invention, the efficient prevention and treatment of root rot are realized through the synergistic effect of strains, the modified carrier improves the stability of viable bacteria, the nutrition conditioner promotes the growth of crops, and the test shows that the prevention and treatment effect reaches 85% or more, the viable bacteria survival rate exceeds 55% after 90 days of storage, and the composition has both disease resistance and growth promotion, and has wide application prospects.

The invention relates to the technical field of soil improvement. The invention provides a saline-alkali soil improving microbial agent and a preparation method thereof. The saline-alkali soil improving microbial agent is prepared from the following raw materials in parts by weight: 15 to 20 parts of bacillus megatherium, 10 to 15 parts of bacillus subtilis, 8 to 12 parts of bacillus mucilaginosus, 5 to 8 parts of pseudomonas fluorescens, 6 to 10 parts of trichoderma harzianum, 5 to 8 parts of arbuscular mycorrhizal fungi and 3 to 5 parts of rhizobium. Through cooperative use of the strains, the salt content of the coastal salinized soil can be effectively reduced, the pH value can be adjusted, the nutrient index of the salinized soil can be improved, the content of microorganisms in the soil can be improved, and the root development of crops can be promoted. Meanwhile, the sectional fermentation process can solve the problem of strain antagonism, and the salt tolerance and viable count of the strain are improved in cooperation with a special composite culture medium, so that the saline-alkali soil improvement effect is further enhanced.

The invention relates to the technical field of medicinal chemistry, in particular to a preparation method of Corey lactone diol. The invention provides a preparation method of Corey lactone diol. The preparation method comprises the following steps: S1, carrying out enzymatic reaction on a compound as shown in a formula II under the action of lipase to obtain a compound as shown in a formula III; s2, hydrolyzing the compound shown in the formula III to obtain a compound shown in a formula I; the lipase is selected from at least one of Candida antarctica lipase, Pseudomonas fluorescens lipase, Burkholderia lipase, Rhizopus mirabilis lipase and porcine pancreatic lipase. The method has the advantages of mild and simple reaction conditions, cheap and easily available raw materials, few reaction byproducts, high chiral optical purity of the product, short overall reaction steps and suitability for industrial production, simplifies the subsequent preparation process of the prostaglandin drugs, and reduces the production cost.

The invention discloses a compound preparation containing nitrogen-fixing bacteria and application of the compound preparation in sugarcane yield increase, and belongs to the technical field of microorganisms. Comprising a complex microbial inoculant and biochar loaded with the complex microbial inoculant, and the complex microbial inoculant is composed of the following strains: Azospirillum brasilense ACCC 04138, Bacillus mucilaginosus ACCC 19749 and Pseudomonas fluorescens CICC 23919. Azospirillum brasilense, bacillus mucilaginosus and pseudomonas fluorescens are compounded to prepare a complex microbial inoculant, and the complex microbial inoculant is specifically combined with biochar prepared from bagasse and mushroom residues, so that the acid soil structure can be remarkably improved, and the sugarcane yield and sugar content can be increased. The invention provides a novel complex microbial inoculant for sugarcane planting, and provides a scientific basis for improvement of sugarcane yield and quality and improvement of acid soil.