168 results about "Pseudomonas fluorescens" patented technology

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention relates to the technical field of microorganisms, in particular to application of streptococcus non-lactolyticus GX-7 and a complex microbial inoculant thereof in sugarcane leaf silage, the strain GX-7 is separated from healthy pig manure by a research group, and through detection, the strain has good cholate resistance and is suitable for surviving in intestinal tracts, and the strain GX-7 has good cholate resistance and is suitable for being used as a feed additive. The antibacterial effect on pseudomonas fluorescens, listeria monocytogenes, salmonella choleraesuis and shigella flexneri is achieved; the strain has a good fermentation capability on sugarcane leaves, and the sugarcane leaves are inoculated with a complex microbial inoculant prepared from streptococcus non-lactolyticus GX-7 and streptococcus non-lactolyticus GX-2, so that a synergistic fermentation effect can be achieved; after the silage prepared from the complex microbial inoculant is fed to female buffalo in the gestation period, it is found that the silage can effectively increase the content of milk protein, milk total solids and milk non-fat solids in colostrum of the female buffalo and relieve diarrhea of the female buffalo, and the silage is safe and good.

The invention relates to a complex microbial inoculant for preventing and treating citrus huanglongbing as well as a preparation method and application of the complex microbial inoculant. The compound bacterial agent for preventing and treating the citrus huanglongbing is prepared from the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. The compound bacterial agent for preventing and treating the citrus huanglongbing comprises the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. After the complex microbial inoculant is added, the content of callose in infected leaves can be effectively reduced, the self-resistance mechanism of plants is induced, the disease resistance is improved, and the complex microbial inoculant has an obvious inhibiting effect and a growth promoting effect on Candidatus Liberibacter asiaticum.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention relates to the technical field of new strains, and provides a new strain which is screened from Xinjiang saline-alkali soil, is classified and named as sphingomonas sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCCNO.32220. Research finds that the strain can remarkably reduce the soil conductivity, the exchangeable sodium content, the alkalization degree and the total salt content, so that the soil quality is improved. The organic matter content and the acid phosphatase activity of the soil are remarkably improved, and the quality of the saline-alkali soil can be efficiently improved. Meanwhile, the invention provides a compound bacterial fertilizer for effectively improving saline-alkali soil, the compound bacterial fertilizer comprises bacillus subtilis, Exiguobacterium acetylbacter, Bacillus velezensis, Pseudomonas fluorescens, Rhodococcus capsulatus and a new strain Sphingomonas sp., and research finds that compared with a single bacterial solution, the compound bacterial solution has a more prominent remediation effect on saline-alkali soil.

The invention relates to a saline-alkaline tolerant microbial seed coating agent and application thereof. The seed coating agent comprises a growth-promoting rhizobacteria inoculant and an auxiliary agent, the volume ratio of the growth-promoting rhizobacteria inoculant to the auxiliary agent is 1: (1-100); in each gram of the seed coating agent, the viable count of the growth-promoting rhizosphere bacteria is (1-100) * 10 < 10 > CFU; the growth-promoting rhizobacteria is selected from one or more of pseudomonas stutzeri A1501, pseudomonas fluorescens ATCC 13525, bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703, and the growth-promoting rhizobacteria is selected from one or more of bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703; on the basis of the total weight of the auxiliary agent, the auxiliary agent is prepared from the following components in percentage by weight: 1 to 9 percent of film forming agent, 1 to 9 percent of protective agent, 0.0001 to 0.001 percent of regulator, 0.0001 to 0.001 percent of active agent, 0 to 25 percent of filler and 58 to 97 percent of water. The seed coating agent can improve the rhizosphere colonization ability and survival rate of rhizosphere growth-promoting bacteria, can also improve the saline-alkaline tolerance of plant seeds, and effectively promotes plant growth.

The invention belongs to the technical field of biological control, and particularly relates to the technical field of crop stem rot control. The invention provides a bacillus velezensis Gc8, and the preservation number of the bacillus velezensis Gc8 is CGMCC (China General Microbiological Culture Collection Center) No. 28991. The bacillus velezensis Gc8 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 14, 2023, the preservation number of the bacillus velezensis Gc8 is CGMCC No.28991, the center is called CGMCC for short, and the address of the center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67, kitasapora fluorescens pf27, kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27. The compound microbial agent can inhibit spore germination of pathogenic microorganisms, and has the effect of promoting growth of corn while resisting diseases.

The invention relates to the field of microorganisms, and discloses a complex microbial inoculant for preventing and treating clubroot of rape. Comprising the following raw materials in parts by weight: 10 to 12 parts of acid-resistant bacillus, 12 to 15 parts of pseudomonas fluorescens PF-5, 8 to 10 parts of bacillus mucilaginosus, 5 to 7 parts of halophilic bacillus, 8 to 10 parts of bacillus amyloliquefaciens, 12 to 15 parts of trichoderma atroviride ReTv2, 6 to 8 parts of azotobacter chroococcum, 10 to 12 parts of chitosan-gamma-polyglutamic acid microspheres, 12 to 15 parts of diatomite and 8 to 12 parts of humic acid. And 2-4 parts of algal polysaccharides. The living space of plasmodiophora brassicae is preempted by the acid-resistant bacillus; the pseudomonas fluorescens PF-5 directly strikes pathogenic bacteria and stimulates the defense system of the oilseed rape; the bacillus amyloliquefaciens releases a plurality of antibacterial substances; the trichoderma atroviride ReTv2 rapidly destroys the structure of the plasmodiophora brassicae in a manner of hyperparasitism and exoenzyme secretion.

The invention provides a microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases, the microbial complex microbial inoculant comprises the following strains by mass: 30%-50% of bacillus subtilis; 20%-40% of bacillus amyloliquefaciens; 10%-30% of pseudomonas fluorescens; 5%-15% of trichoderma harzianum; and 2%-5% of an auxiliary agent. According to the microbial complex microbial inoculant disclosed by the invention, a multiple prevention and control mechanism of'bacteriostasis-competition-parasitism 'is constructed through the synergistic effect of various strains, and meanwhile, the double-layer effect of thallus protection and function enhancement is realized through the assistance of the auxiliaries, so that the prevention and control effect of the prepared microbial complex microbial inoculant is greatly improved.

The invention relates to a composite microbial agent suitable for saline-alkali soil remediation of sorghum production in northeast alpine regions, and a preparation method and application thereof. The composite microbial agent comprises bacillus subtilis, bacillus mucilaginosus and pseudomonas fluorescens. Bacillus mucilaginosus capable of efficiently producing polyglutamic acid and potassium and pseudomonas fluorescens capable of secreting chitinase are separated and screened from obstacle soil in northeast alpine regions, and field trials are carried out in the obstacle soil (coastal saline-alkali soil) in the northeast alpine regions. The low-temperature slow-release fertilizer has the unique characteristics of secreting polyglutamic acid, potassium and chitinase under the low-temperature condition, is low in cost, and can effectively improve the physical and chemical properties of soil, increase the seed emergence rate, stimulate the root growth and enhance the salt resistance and alkali resistance of seedlings after being applied.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The invention relates to the technical field of microorganisms, in particular to application of streptococcus non-lactolyticus GX-2 and a complex microbial inoculant thereof in peanut vine silage, and the strain GX-2 is suitable for surviving in the stomach and has an antibacterial effect on staphylococcus aureus, escherichia coli, pseudomonas fluorescens and listeria monocytogenes; the streptococcus non-lactolyticus GX-7 and the streptococcus non-lactolyticus GX-2 are prepared into a complex microbial inoculant, and then the complex microbial inoculant is inoculated into the peanut vines, so that the synergistic fermentation effect can be achieved at the mass ratio of 1: (1-3); after the gestational buffalo is fed with the silage prepared from the complex microbial inoculant, the silage is found to be capable of effectively increasing the contents of total milk solids and non-fat milk solids in the colostrum of the buffalo and relieving the diarrhea of the buffalo, and is safe and good.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention provides an acid-blocking and aluminum-controlling composite modifier and a preparation method and application thereof, the modifier is composed of microcrystalline activated minerals, a silicon-calcium material, a biomass alkaline material and a microbial agent, and the microbial agent comprises rhizobium, trichoderma harzianum and pseudomonas fluorescens. The specific raw material ratio can be adjusted according to different requirements, and the effect of materials with different ratios on improving the soil acidity and improving the crop yield and quality is researched by adopting field trials, so that an acid soil conditioner formula is formed; the preparation method comprises the steps of ultrafine grinding, microcrystal activation, hybridization, mixing and the like, the microcrystal activation adopts an activator solution for treatment, and an organic binder is added in the hybridization process to form an organic-inorganic hybrid structure. The composite modifier can effectively improve the physical action effect and the chemical reaction speed of the acid conditioner, and can be used for improving acid soil.

The invention relates to the technical field of fertilizers, and particularly discloses a bacterial strain capable of reducing the function of vomitoxin of wheat and corn and application of the bacterial strain, the bacterial strain capable of reducing the function of vomitoxin of wheat and corn is applied to preparation of a fertilizer, 10%-90% of bacillus velezensis XNYF-1 fermentation liquor (A liquor) and 10%-90% of pseudomonas fluorescens XNYF-2 fermentation liquor (B liquor) are mixed to prepare AB mother liquor, and the AB mother liquor is used for preparing the fertilizer. The XNYF-1 and the XNYF-2 are two functional microbial strains which are subjected to screening mutagenesis and can be used for producing chitinase, iturin, various phenazine derivatives and biological surfactin respectively, and the XNYF-1 and the XNYF-2 can be used for producing the chitinase, the iturin, the various phenazine derivatives and the biological surfactin respectively. And the metabolite has a good biocontrol effect.

The invention belongs to the technical field of functional grease processing, and particularly relates to an enzymolysis preparation method and application of diglyceride with an effect of improving Alzheimer's disease. The method comprises the following steps: mixing low-erucic acid rapeseed oil, glycerol and water, and heating to obtain a pre-reaction mixture; adding compound enzyme for reaction, and then adding candida antarctica lipase B and pseudomonas fluorescens lipase for reaction; and after the reaction is completed, carrying out molecular distillation, collecting a light-phase product, carrying out adsorption filtration, and collecting filtrate to obtain the diglyceride oil. The compound enzyme is composed of rhizomucor miehei lipase, candida rugosa lipase and candida antarctica lipase B in a weight ratio of (2-4): 2: 1. According to the method, the content of diglyceride is effectively increased, the prepared composition has the efficacy of preventing and treating Alzheimer's disease, and the efficacy is shown as improvement of reaction capacity and cognitive function, inhibition of brain apoptosis and acetylcholin esterase, enhancement of SOD and CAT vitality and improvement of related gene expression.

The invention relates to the technical field of fertilizers, in particular to a fulvic acid type compound microbial organic fertilizer and a preparation method thereof. The method comprises the following steps: preparing compound microorganism bacterium powder from bacillus subtilis, bacillus megatherium, bacillus licheniformis, pseudomonas fluorescens and bacillus psychrosaccharolyticus; preparing a solid-phase substance from ferric chloride hexahydrate, terephthalic acid and dimethylformamide; generating hydrogel on the surface of the chemical fertilizer in situ, and adding a solid-phase substance to obtain a slow-release chemical fertilizer; and preparing an organic fertilizer, and mixing the organic fertilizer with the compound microbial powder, the slow-release chemical fertilizer and the organic fertilizer to obtain the compound microbial organic fertilizer. According to the compound microbial organic fertilizer provided by the invention, the chemical fertilizer, the organic fertilizer and the microbial fertilizer synergistically promote the growth of plants, and the use of the chemical fertilizer is reduced.

The invention discloses a microbial agent for promoting sand fixation in sand land and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises bacillus megatherium, pseudomonas fluorescens, lactobacillus mucus, trichoderma harzianum and azotobacter chroococcum which are mixed in the form of bacterial liquid, the volume ratio of the bacterial liquid is (3-5): (2-3): (2-3): (1-2): (1-2), and all bacterial strains have a synergistic effect. When the microbial agent is applied to soil, the soil stability is obviously improved after the microbial agent is applied, the water retention rate of sand land soil can be improved by 15.9 times within 10 days, the sand rising wind speed reaches 18.5 m / s and above, and the vegetation coverage can reach 32% and above after one year. The microbial agent disclosed by the invention is simple and convenient in preparation method, can remarkably promote sand fixation in the sand land and improve the survival rate of plants in the sand land, has the characteristics of environmental protection and high efficiency, and is easy to popularize and apply.

The invention belongs to the technical field of microorganisms, and particularly discloses lactobacillus plantarum CX1-3-2 capable of producing gamma-aminobutyric acid and improving sleep and application of the lactobacillus plantarum CX1-3-2. The preservation number of the lactobacillus plantarum CX1-3-2 strain is CGMCC (China General Microbiological Culture Collection Center) No. 34340. The lactobacillus plantarum CX1-3-2 can achieve the effect of improving sleep by improving the levels of GABA and 5-HT in brain tissues and reducing the levels of glutamic acid Glu, dopamine DA and noradrenaline NE; the scavenging rate of DPPH free radicals of the strain reaches 96.4%, the scavenging rate of ABTS free radicals of the strain reaches 78.3%, and the strain has an excellent anti-oxidation effect; the diameters of inhibition zones of the strain on staphylococcus aureus, bacillus cereus, escherichia coli and pseudomonas fluorescens are all larger than 17 mm, and the strain has an excellent antibacterial effect.

The invention discloses a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent comprises a compound fungal agent and a compound bacterial agent, the compound fungus fungicide is prepared from Cunninghamella echinacea, Lachnum sp., Acremonium sp., Saccharomyces cerevisiae, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Saccharomyces cerevisiae and Saccharomyces cerevisiae. The composite bacterial agent is prepared from Serratia sp., Pseudomonas fluorescens, Bacillus spp., Streptomyces ardesiacus, and Streptomyces sp., and the composite bacterial agent is prepared from the following raw materials: Serratia sp., Pseudomonas fluorescens, Bacillus spp., Streptomyces ardesiacus, and Streptomyces spp., and the composite bacterial agent is prepared from the following raw materials: Saccharomyces cerevisiae, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Saccharomyces cerevisiae and Saccharomyces cerevisiae. The prepared compound microbial agent has a remarkable promoting effect on growth of artificially cultivated rhizoma atractylodis and accumulation of medicinal active ingredients, and the effectiveness of the compound microbial agent is proved in pot experiments and field experiments. The method is low in operation cost, has universality and is easy for large-scale production.

The invention relates to the technical field of agricultural microbial preparations, in particular to a complex microbial inoculant for promoting severe salt and alkali resistance of crops as well as a preparation method and application of the complex microbial inoculant. Effective viable bacteria in the complex microbial inoculant comprise bacillus licheniformis with the cell count content of 26%-41%, bacillus subtilis with the cell count content of 31%-46% and pseudomonas fluorescens with the cell count content of 21%-36%. When the microbial agent is applied to plant root systems of soda saline-alkali soil containing sodium carbonate and sodium bicarbonate, the saline-alkali resistance of plants can be improved, and the plant height, ground diameter, fresh weight, dry weight, root length and root number of the plants are remarkably increased; besides, the complex microbial inoculant can effectively improve the growth of saline-alkali soil crops and solve the difficult problem of soda saline-alkali soil remediation in the field by improving the activity of antioxidant enzymes, increasing the content of chlorophyll, helping plants maintain high photosynthetic activity and relieving the damage of saline-alkali stress to a plant photosynthesis system.

The invention relates to the technical field of calcium carbonate manufacturing, in particular to a preparation method of high-whiteness light calcium carbonate. Comprising the following steps: efficiently leaching the calcium element in the carbide slag; preparing a microbial crystal form control agent; and carrying out electric dispersion treatment and precipitation on calcium ions. The microbial crystal form control agent is prepared by regulating and controlling the mixing ratio of the bacillus pumilus fermentation liquor to the pseudomonas fluorescens fermentation liquor, and it is avoided that in the full mixing process of the microbial crystal form control agent and the calcium-containing filtrate, Ca < 2 + > is mineralized to form large calcium carbonate particles; stearic acid and sodium polyacrylate in the microbial crystal form control agent can enable particles to be more stable, provide ion adsorption capacity, optimize the crystal form of calcium carbonate and form vaterite type crystals with smaller crystal form, and bacillus pumilus can assist generation of small-particle-size calcium carbonate along with time, so that high-whiteness small-particle-size light calcium carbonate is obtained.

The invention discloses a moss-microorganism synergistic nitrogen fixation bionic regreening method and system for a drought-resistant mine rock slope, and belongs to the technical field of ecological environment restoration. The method comprises the following steps: (1) slope pretreatment: drilling anchoring holes; (2) layered construction: (1) a fixation layer: spraying mixed slurry prepared from amylase and plant fibers to form a net-shaped substrate with the thickness of 1-2cm; (2) a nutrient layer: spraying a mixture of a complex microbial inoculant and a slow release fertilizer, wherein the thickness is 4cm; the mixed bacterial agent is prepared from the following raw materials in percentage by weight: 60 percent of phytonimum patenum spores, 25 percent of nostoc commune (Nostoc commune) and 15 percent of pseudomonas fluorescens (Pseudomonas fluorescens), and the mixed bacterial agent is prepared from the following raw materials in percentage by weight: 60 percent of phytonimum patenum spores, 25 percent of nostoc commune (Nostoc commune) and 15 percent of pseudomonas fluorescens (Pseudomonas fluorescens). And (3) a water retention layer: sodium polyacrylate resin is spread, the particle size of the sodium polyacrylate resin is 1-0.5 mm, soil covering and compaction are performed until the thickness of the water retention layer is 1-1.5 cm, and the use amount of the sodium polyacrylate resin is 2-1.5 wt% of the weight of the soil. According to the method, the nitrogen fixation efficiency can be improved by 40-60%, and the soil moisture content is kept gt under the drought condition; the survival rate of the seedlings is increased to 85% or above, the anti-scouring capacity of the side slope is improved by 50%, and the vegetation coverage rate of 70% or above is achieved within 6 months.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for heavy metal contaminated soil and a biological bacterial fertilizer and a preparation method thereof. The micro-ecological restoration liquid complex microbial inoculant suitable for the heavy metal contaminated soil comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azotobacter braziliensis CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and restoration bacteria: pseudomonas putida CGMCC 1.4533. The biological bacterial fertilizer for the heavy metal contaminated soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the heavy metal contaminated soil is greatly improved, the heavy metal contaminated soil is effectively improved, and the crop yield is remarkably increased.

The invention provides a material supplementing method for producing mupirocin through fermentation of pseudomonas fluorescens. The method specifically comprises the following steps: S1, preparing a material supplementing system: preparing material supplementing liquid, and adjusting the pressure difference between a material supplementing tank and a fermentation tank; s2, monitoring material supplementing trigger conditions: monitoring the dissolved oxygen content and the pH value in the fermentation tank in real time through a PLC control system; s3, executing a feeding program: starting a peristaltic pump to feed a feeding liquid in a quantitative feeding mode, and feeding ammonia water; and S4, dynamic feedback adjustment: controlling the flow of glucose supplement by adjusting the rotating speed of a peristaltic pump, controlling specific dissolved oxygen saturation in different fermentation stages by adjusting the ventilation quantity and the stirring rotating speed, and controlling specific pH values in different fermentation stages by supplementing ammonia water. According to the present invention, by designing the material supplementing liquid with the specific raw materials and the specific ratio, adopting the peristaltic pump to quantitatively supplement the material, and detecting and controlling the glucose content, the dissolved oxygen and the pH value during the fermentation process, the strain can grow and metabolize in the stable environment, the improvement of the mupirocin yield is promoted, the fermentation period is shortened, and the energy consumption is reduced.

The invention belongs to the technical field of biological phosphorus-silicon fertilizers, and particularly relates to a liquid complex microbial inoculant capable of repairing heavy metals and activating phosphorus and silicon and a biological phosphorus-silicon fertilizer thereof. The liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria Cupriavidus and Ochrobactrum, nitrogen-fixing bacteria Azospirillum brasilense, phosphate solubilizing bacteria Bacillus megaterium and Pseudomonas, potassium solubilizing bacteria Bacillus mucilaginosus, growth-promoting bacteria Pseudomonas fluorescens, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants and microbial inoculants. The biocontrol bacteria are trichoderma harzianum and bacillus subtilis, and the synergistic bacteria are saccharomyces cerevisiae; the biological phosphorus-silicon fertilizer is prepared from the liquid complex microbial inoculant. According to the biological phosphorus-silicon fertilizer provided by the invention, through scientific compatibility of various functional strains, heavy metal remediation and soil phosphorus and silicon solubilization are realized, absorption of phosphorus and silicon in soil is promoted, and the crop yield is increased.

This invention belongs to the field of bio-mineral fertilizer technology, specifically relating to a microbial liquid compound agent and bio-mineral fertilizer that combine coal gangue activation and heavy metal remediation. The microbial liquid compound agent comprises the following functional strains prepared through three-stage sequential inoculation and fermentation using electrochemical low-Hertz water: *Pseudomonas putida* (a remedial bacterium), *Azotobacter chrysogenum* (a nitrogen-fixing bacterium), *Azospirillum brasiliensis* (a growth-promoting bacterium), *Pseudomonas fluorescens* (a phosphate-solubilizing bacterium), *Bacillus subtilis* (a biocontrol bacterium), *Bacillus mucilaginosus* (a potassium-solubilizing bacterium), *Bacillus megaterium* (a silica-solubilizing bacterium), and *Trichoderma echinococcus* (a synergistic bacterium). The bio-mineral fertilizer is prepared using the aforementioned microbial liquid compound agent, with coal gangue powder as the main raw material. The scientific combination of different strains provided by this invention achieves efficient improvement of heavy metal-contaminated land and increased crop yields. Simultaneously, it transforms coal gangue into a valuable resource, solving the pollution problem caused by the large-scale accumulation of coal gangue, and possesses significant economic and social value.

The invention relates to the technical field of biology, in particular to pseudomonas fluorescens GS-1 for synthesizing polyhydroxyalkanoate and application of the pseudomonas fluorescens GS-1, the pseudomonas fluorescens GS-1 is preserved in China General Microbiological Culture Collection Center on February 6, 2026, and the preservation number is CGMCC No.37739; the strain carries a phaC gene and can synthesize PHA, and the straw sugar PHA conversion efficiency reaches 87.7% of that of a glucose system; the method has inherent lignocellulose hydrolysate inhibitor tolerance, and the PHA synthesis capability retention rate in the complex self-made corn straw saccharification liquid reaches 91.4%. A high-quality strain resource with direct application potential is provided for producing the PHA based on cheap straw saccharification liquid.

The invention discloses a construction method of a high-yield siderophore pseudomonas fluorescens overexpression strain constructed based on ARTP mutagenesis, and the construction method of the strain comprises the following steps: (1) screening based on ARTP mutagenesis to obtain a siderophore synthesis positive regulation gene rpoS mutant gene sequence which is SEQ ID NO.1; and (2) constructing a pseudomonas fluorescens mutant strain with high siderophore yield. According to the obtained pseudomonas fluorescens overexpression strain, the siderophore yield reaches 89.8%. According to the invention, the siderophore is screened according to the gene mutation proportion to synthesize the positive regulation gene, and the screened rpoS mutant gene is overexpressed into the pseudomonas fluorescens, so that the RpoS protein in a high expression state promotes the synthesis and transport of the siderophore, and the high yield of the siderophore is realized.

The invention relates to an artificial phosphate-solubilizing module and application thereof in improving the nitrogen-fixing and phosphate-solubilizing capabilities of nitrogen-fixing microorganisms. The artificial phosphate solubilizing module sequentially comprises an inducible promoter element and a conservative acid phosphatase gene acpA from a pseudomonas fluorescens strain from upstream to downstream. The artificial phosphate solubilizing module is inserted into a preset target site of a host microorganism chromosome in a homologous recombination manner, so that a host has the characteristic of efficiently expressing acid phosphatase, and the organic phosphorus degradation capacity and nitrogen fixation capacity of the host are remarkably improved. The method has high application value in the field of preparation of high-efficiency microbial fertilizer products. The method opens up a new path for agricultural green sustainable development, and has extremely important scientific significance and wide application prospects.