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Microorganism which reduces nitrosamines and method of reducing nitrosamines using the same

A technology of microbes and microbiological treatment, applied in the direction of microbe-based methods, biochemical equipment and methods, methods using bacteria, etc., can solve problems such as tobacco quality problems, tobacco leaf quality, odor and taste adverse effects, and achieve the goal of maintaining quality Effect

Inactive Publication Date: 2005-08-10
JAPAN TOBACCO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has been pointed out that microorganisms belonging to the genus Aspergillus require a high moisture content to survive, and this high moisture content can adversely affect the quality of tobacco leaves, especially its odor and taste
Therefore, the application of such microorganisms as described above in the treatment of tobacco leaves can cause problems in tobacco quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1) Isolation of microorganisms from tobacco leaves

[0046] Microorganisms were collected from tobacco leaves grown on fields in Oyama-shi, Tochigi Prefecture.

[0047] Microorganisms were collected 3 times, namely immediately after harvesting the tobacco leaves, on day 3 of the curing period (i.e. when the color of the leaves had completely turned yellow) and on day 8 of the curing period (i.e. when the color of the leaves had completely turned brown). Time).

[0048] Stem parts of Michinoku 1 belonging to Burley tobacco were harvested and the leaves of the harvested tobacco leaves were cut short as samples. The sample thus obtained was finely cut into a square of 5 mm x 5 mm. From this about 10 g of sample was placed in a 300 ml Erlenmeyer flask. 200 ml of 10 mM phosphate buffer (pH: 7.0) was added thereto and the mixture was homogenized. The suspension thus obtained was used as "suspension at harvest" containing microorganisms derived from tobacco leaves at harve...

Embodiment 2

[0106] Example 2: Reduction of TSNA content during ripening by treatment with LG38 strain

[0107] The LG38 strain was inoculated into 1 / 10 TS broth and cultured at 30°C for 72 hours. After the cultivation, the culture medium containing the bacterial cells was centrifuged at 5000 rpm to separate and collect the bacterial cells. The bacterial cells thus collected were washed twice with sterile distilled water and suspended in sterile distilled water. The concentration of microorganisms in the suspension was adjusted to 10 by diluting the suspension with sterile distilled water. 8 -10 10 cfu / ml.

[0108] Tobacco leaves of Burley tobacco (Kitakami 1 ), which had been harvested and subjected to a curing process, were treated with the microbial suspension thus adjusted to a predetermined concentration. Three treatments were performed, ie immediately post-harvest, 3 days post-harvest and 8 days post-harvest. In each treatment, the suspension was sprayed on the upper and lower s...

Embodiment 3

[0129] Example 3: Effect of treatment with LG5 strain on NNK content

[0130] NNK content was determined in a manner similar to that described in Example 2, except that the microorganism used in the method of Example 2 was replaced by LG5.

[0131]The results are shown in Table 7.

[0132] Table 7

[0133] time after harvest (days)

deal with

day 10

day 21

day 32

Untreated (control group)

0.15

0.52

0.29

water treatment

0.71

0.61

0.32

LG5 strain treatment

0.59

0.78

0.22

[0134] As evident from Table 7, in the group that received no treatment, the NNK content increased up to day 21, while the NNK content on day 32 (when the aging process was completed) was lower than that on day 21. In both groups treated with water and with the LG5 strain, NNK levels remained relatively high up to day 21, whereas in the combination that re...

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Abstract

The invention relates to a method for reducing the SNA content in tobacco leaf, which is characterized in adopting microorganism for treatment. The microorganism is selected from the group formed with sphingomonas paucimobolis and pseudomonas fluorescens and can reduce the SNA content.

Description

technical field [0001] The present invention relates to a microorganism for degrading nitrosamines formed in tobacco leaves during curing and storage of tobacco leaves and a method for reducing nitrosamines formed in tobacco leaves during curing and / or storage by using the microorganisms. Background technique [0002] The nitrosamines contained in tobacco leaves (tobacco-specific nitrosamines, hereinafter referred to as "TSNA") do not exist in the tobacco leaves just after harvesting (ie green leaves), but pass through the tobacco leaves during the curing and storage process Formed by the reaction between the contained alkaloids and nitriles. This nitrile is formed by microorganisms that are present on the surface of the tobacco leaf and have the ability to reduce nitrate. [0003] The main TSNAs formed during aging and subsequent storage are N'-nitrosonornicotine (hereinafter "NNN"), 4-(N-nitrosomethylamino)-1-(3-pyridyl) -1-Butanone (hereinafter r...

Claims

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Application Information

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IPC IPC(8): A24B15/20C12R1/01C12R1/39
CPCA24B15/20C12R1/39C12R1/01A24B15/245C12N1/205C12R2001/01C12R2001/39
Inventor 古贺一治胜屋聪
Owner JAPAN TOBACCO INC
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