101 results about "Nitrosamine" patented technology

The invention provides a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) detection method for N-nitroso landiolol in a landiolol hydrochloride bulk drug. The method comprises the step of carrying out qualitative detection or quantitative analysis on a potential nitrosamine impurity-N-nitroso landiolol in a landiolol hydrochloride bulk drug by adopting an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), wherein the structural formula of the N-nitroso landiolol is shown as a formula I in the specification. The detection method disclosed by the invention is high in sensitivity, strong in specificity and good in repeatability, can be used for rapidly and effectively detecting N-nitroso landiolol in the landiolol hydrochloride bulk drug, and has important significance on quality control of the landiolol hydrochloride bulk drug. ; formula I.

The present invention relates to the use of ascorbic acid fatty acid esters for reducing nitrosamine formation in compositions comprising methylphenidate.

Provided are compositions comprising certain miglustat-related nitrosamine compounds, such as pharmaceutical compositions comprising miglustat and such miglustat-related nitrosamine compounds. Also provided are pharmaceutical compositions comprising miglustat and less than certain levels of such miglustat-related nitrosamine compounds. Also provided are methods of analyzing such miglustat-related nitrosamine compounds.

This invention discloses a strain of *Lactobacillus rhamnosus* H7 and its applications, belonging to the field of food microbiology technology. This invention provides a *Lactobacillus rhamnosus* H7 isolated from fermented meat, deposited at the Guangdong Provincial Microbial Culture Collection Center. This strain has the function of degrading nitrite. When inoculated into dried sausage, the residual nitrite and nitrosamine levels in the dried sausage are reduced, and the color of the meat product is significantly improved. When inoculated into fermented mandarin fish, the residual nitrite and nitrosamine levels in the fermented mandarin fish are also reduced. Therefore, inoculating fermented meat products with *Lactobacillus rhamnosus* H7 not only helps improve the safety of fermented meat products but also enhances their quality. This strain has good application prospects as a starter culture for fermented meat products.

The invention relates to a method for determining the content of nitrosamine impurities in a pramipexole dihydrochloride sustained-release tablet, which comprises the following steps: grinding the pramipexole dihydrochloride sustained-release tablet into fine powder as a sample to be detected, and extracting with a diluent to obtain a test solution; and carrying out liquid chromatography-mass spectrometry detection on the test solution, and calculating according to the detection result to obtain the content of the nitrosamine impurity N-nitroso-pramipexole in the pramipexole dihydrochloride sustained release tablet. According to the method, the nitrosamine impurity N-nitroso-pramipexole can be effectively extracted from the pramipexole dihydrochloride sustained release tablet, and the nitrosamine impurity is quantitatively detected by liquid chromatography-mass spectrometry, so that the problems that the nitrosamine impurity N-nitroso-pramipexole is low in content, difficult to detect and easy to interfere are successfully solved. The method is high in accuracy, precision and sensitivity and good in linearity, specificity and system adaptability.

This invention discloses a method for the deamination functionalization of aliphatic nitrosamines, comprising: reacting an aliphatic nitrosamine as shown in formula (II) with a nucleophile in the presence of an acid and a solvent to generate a deamination functionalized product as shown in formula (III); wherein, R 1 R 2 R 3 Each group is independently selected from hydrogen, hydroxyl, alkyl, alkoxy, alkanoyl, alkoxycarbonyl, alkylsulfinyl, alkylsulfonyl, aryl, heteroaryl, phenylalkyl, or R 1 R 2 R 3 Two or three of the functional groups form a non-aromatic ring, cage-like compound; FG is a functional group in which a nucleophile participates in the reaction. The method of this invention has the advantages of inexpensive and readily available raw materials, simple reaction conditions, and wide substrate applicability.

The application belongs to the technical field of substance analysis and detection, and provides an extraction method and a detection method for N-nitrosamine compounds in aquatic products. The N-nitrosamine compounds in the aquatic products to be measured are extracted by steam distillation, and the extraction rate of the N-nitrosamine compounds is high. The obtained distillate is purified and enriched by using an acid-modified coconut shell activated carbon column, the acid-modified coconut shell activated carbon column can effectively adsorb N-dimethyl-nitrosamine and N-diethyl-nitrosamine in the distillate, the acid-modified coconut shell activated carbon column is first eluted with n-hexane to remove part of the fat-soluble impurities, then N-dimethyl-nitrosamine and N-diethyl-nitrosamine are eluted with dichloromethane, due to the strong adsorption of the acid-modified coconut shell activated carbon column to the impurities, the impurities are not easily eluted by dichloromethane, so that the impurities, N-dimethyl-nitrosamine and N-diethyl-nitrosamine are effectively separated, and the purpose of purification is achieved.

Provided are compositions comprising certain miglustat-related nitrosamine compounds, such as pharmaceutical compositions comprising miglustat and such miglustat-related nitrosamine compounds. Also provided are pharmaceutical compositions comprising miglustat and less than certain levels of such miglustat-related nitrosamine compounds. Also provided are methods of analyzing such miglustat-related nitrosamine compounds.

This invention discloses a sophoridine tricyclic derivative or its pharmaceutical salt, with the following general structural formula: R1 is selected from hydrogen, C1-C20 straight-chain alkyl, and C1-C20 branched alkyl. In vitro experiments have verified that the sophoridine tricyclic derivative can inhibit TGF-β-induced activation of hepatic stellate cells, improve CCl4- and bile duct ligation-induced liver injury and liver fibrosis in mice, inhibit TGF-β / Smads signaling pathway activation, and also inhibit diethylnitrosamine-induced primary liver cancer in mice. Therefore, this invention provides new evidence for the treatment, alleviation, or improvement of liver fibrosis or primary liver cancer using sophoridine tricyclic derivatives.

The application relates to the technical field of chemical analysis, and discloses a method for extracting, separating and detecting N-nitrosamine, which comprises the following steps: a sample obtaining step; a sample purification step; a preparation and extraction solution step; an extraction solution separation step; an extraction solution concentration step; a chromatographic detection and quantitative analysis step; the sample to be detected is crushed to a size of 0.01g of powder, and the powder is mixed with sodium chloride, barium hydroxide and water. In the application, the sample to be detected is subjected to a step-by-step sample treatment process before detection, so that the interference factors of the sample to be detected can be effectively reduced, the influence on the detection instrument is reduced, the detection result and detection data are more accurate, and when the application is detected, a large amount of sample does not need to be weighed like in the traditional detection mode, the overall operation is more convenient, and the detection demand of N-nitrosamine in smoked food is met.

The invention discloses an epinephrine drug nitrosamine impurity as well as a preparation method and application thereof. According to the method, an epinephrine drug is taken as a raw material, hydroxyl is protected through a silane protecting group, the protected hydroxyl and a nitrosation reagent are subjected to a selective nitrosation reaction, and then the target nitrosamine impurity is obtained through a deprotection reaction. According to the method, the high-purity nitrosamine impurity of the epinephrine medicine can be prepared and used as a reference substance for qualitative and content detection of the nitrosamine impurity in the production process of raw materials and medicines, so that it is ensured that the epinephrine raw material medicine and preparations meet the medicinal standard; the method has important significance in improving the quality of epinephrine drugs and guaranteeing the medication safety.

A method for adsorbing tobacco-specific nitrosamines from a tobacco extract. The method comprises: 1) diluting a tobacco extract with a solvent; 2) adding activated carbon to the diluted tobacco extract, followed by static adsorption; 3) subjecting the tobacco extract, which has been adsorbed by activated carbon, to first centrifugation and then first filtration to obtain a first filtrate; 4) adding a molecular sieve to the first filtrate for stirring and adsorption; 5) subjecting the tobacco extract, which has been adsorbed by the molecular sieve, to second centrifugation and then second filtration to obtain a second filtrate; and 6) concentrating the second filtrate under reduced pressure into a tobacco extract that has been concentrated under reduced pressure having the same solid content as the tobacco extract, wherein the content of tobacco-specific nitrosamines in the tobacco extract that has been concentrated under reduced pressure is less than 70000 ng / g. In addition, a tobacco extract obtained by the method and an atomizing matrix comprising the tobacco extract are provided.

This application discloses an analytical method for N-nitrosamine vortioxetine in vortioxetine hydrobromide tablets, belonging to the field of pharmaceutical impurity analysis. The method employs high-performance liquid chromatography (HPLC), with the following chromatographic conditions: a 4.6 mm × 250 mm column packed with octadecylsilane-bonded silica gel, with a particle size of 5 μm; mobile phase A is a 90:10 water-acetonitrile mixture containing 0.025%–0.035% trifluoroacetic acid (v / v); mobile phase B is acetonitrile; gradient elution; column temperature 38–42 °C; flow rate 0.9–1.1 ml / min; injection volume 50 μl; and detection wavelength 226 nm. This analytical method effectively eliminates excipient interference and exhibits excellent detection performance (specificity, sensitivity, linearity, precision, accuracy, and robustness), accurately detecting the content of N-nitrosamine vortioxetine in vortioxetine hydrobromide tablets, meeting quality control requirements.

The present invention provides an oral composition comprising an Lp(a) inhibitor compound having a reduced level of nitrosamine. [Solution] An oral composition comprising muvalaprin and histidine is used, wherein novel polymorphs of muvalaprin, such as muvalaprin hydrate form A, muvalaprin hydrate form B, muvalaprin hemihydrate, and muvalaprin hydrate 1:6.5 molar equivalents, muvalaprin hemihydrate and / or muvalaprin hydrate 1:6.5 molar equivalents are used. Furthermore, the composition provides a method for treating cardiovascular disease, a method for treating atherosclerotic cardiovascular disease, and / or a method for lowering Lp(a) levels in an individual by administering one of the oral compositions.

The invention provides a method for detecting the genetic toxicity of nitrosamine compounds, and belongs to the technical field of biological detection. According to the method, key conditions of a detection system are systematically optimized, so that the detection sensitivity and the result reliability of the genotoxicity of the nitrosamine compounds are remarkably improved, and the mutagenicity which is difficult to detect under conventional conditions can be stably and clearly identified; the establishment of the method provides powerful technical support for early screening and safety risk assessment of nitrosamine impurities in drugs and chemicals, and has important practical application value for guaranteeing medication safety and product quality.

This invention relates to the field of pharmaceutical technology, specifically proposing the application of ligustilide in the preparation of drugs for inhibiting and / or preventing and / or alleviating and / or treating lung tumors to address the current lack of natural drugs for lung cancer prevention. Lung tumors are nitrosamine-induced carcinogenesis of lung tissue. The invention relates to a pharmaceutical preparation for inhibiting and / or preventing and / or alleviating and / or treating lung tumors, comprising an active ingredient and pharmaceutically acceptable excipients; wherein the active ingredient is ligustilide. Ligustilide directly targets the STING protein, activates the cGAS-STING pathway, promotes STING phosphorylation and cell-mediated immune surveillance, while simultaneously regulating ROS homeostasis and the inflammatory microenvironment, reducing nitrosamine damage to lung epithelial cells, and preventing nitrosamine-induced lung carcinogenesis.

The invention provides a processing method for inhibiting and reducing N-dimethyl nitrosamine (NDMA) in grilled cod fillets. The processing method comprises the following steps: storing a semi-finished product prepared from fresh cod fillets at a low temperature for not less than 8 days, unfreezing the semi-finished product to room temperature, grilling the semi-finished product at 165-175 DEG C for 3-5 minutes until the semi-finished product is brown, and finally performing air blowing treatment at room temperature for 10-30 minutes. According to the method, three key parameters of semi-finished product storage, baking temperature and air blowing treatment are accurately controlled, so that an optimal process combination is obtained, the lowest residue of NDMA is realized, and the synergistic interaction effect of a full-chain comprehensive strategy of'source control-process blocking-terminal reduction 'in production and processing of the grilled cod fillets is reflected. According to the method, the level of hazardous substances is effectively controlled, NDMA is effectively reduced, under the condition that product quality is not affected, feasibility and economical efficiency of production are considered to the maximum extent, and an optimization solution with safety guarantee and cost effectiveness is provided for the industry.

According to the marine rhodotorula mucilaginosa and the method for mixed inoculation of the marine rhodotorula mucilaginosa and the lactobacillus rhamnosus, the selected marine rhodotorula mucilaginosa can efficiently degrade NDMA in air-dried sausages and smelly mandarin fish, various nitrosamines in fermented meat products can be jointly degraded through the synergistic effect of the marine rhodotorula mucilaginosa and the NDMA in the smelly mandarin fish, in addition, the mixed bacteria synergistically show an excellent degradation effect on biogenic amines, and the marine rhodotorula and the degradation efficiency is far higher than that of single-bacterium fermentation. In the invention, the combination of adaptive directed evolution and mutation breeding is used for improving the nitrosamine degradation capability of the strain for the first time, the marine rhodotorula mucilaginosa SY-AN-9 obtained by the technology is applied to degradation of nitrosamine in fermented meat products for the first time, and a method for synergistically degrading biogenic amine and nitrosamine by mixed bacteria is developed. The safety of the fermented meat product is improved, the application range of the marine rhodotorula mucilaginosa and the rhamnosus lactobacillus is widened, and the application of the marine rhodotorula mucilaginosa and the rhamnosus lactobacillus in the food industry is promoted.

This application provides a quantitative method for the derivatization of nitrogen nitrosamines and its application. Through simple derivatization, nitrogen nitrosamines with different structures can be determined. In the embodiments of this application, the sample derivatization method is studied, and optimal reaction conditions for nitrogen nitrosamines are proposed. This application establishes a simple and sensitive liquid chromatography quantitative analysis method, successfully establishing a method for the derivatization analysis of nitrogen nitrosamines with different structures. This method has high selectivity, sensitivity, and accuracy, and is suitable for the accurate determination of nitrogen nitrosamines in complex samples and complex structures.

The invention discloses a method for determining N-nitrosamine compounds in animal-derived food, and belongs to the field of food detection. The method comprises the following steps: freezing and grinding animal-derived food, mixing the animal-derived food with an internal standard solution, deionized water and acetonitrile, and freezing and preserving to obtain a pretreated sample; the method comprises the following steps: treating a pretreated sample through a QuEChERS salt bag, centrifuging, carrying out nitrogen blowing concentration, and mixing with deionized water to prepare an enriched solution; purifying the enriched solution by adopting a purification bag or specific functional resin, and centrifugally filtering to obtain a sample solution; and calculating the content of the target compound through a peak area ratio by using a liquid chromatograph-high resolution mass spectrometer provided with an APCI source and taking N-nitrosodimethylamine-D6 as an internal standard. The functional resin is formed by cross-linking specific functional monomers and is large in specific surface area and high in adsorption selectivity; the method has strong anti-interference capability, and can efficiently detect the N-nitrosamine compounds in dried aquatic products and other animal-derived foods.

The invention belongs to the technical field of pharmaceutical preparations, and relates to a levoamlodipine folic acid compound tablet and a preparation process thereof, and the preparation process comprises the following steps: mixing microcrystalline cellulose with vitamin C, and enabling nitrite in the microcrystalline cellulose to be in full contact with the vitamin C to obtain a first mixture; mixing folic acid with part of the first mixture to obtain a second mixture; mixing levamlodipine besylate with the remaining first mixture to obtain a third mixture; and uniformly mixing the second mixture, the third mixture and other auxiliary materials, and tabletting to obtain the tablet. The method comprises the following steps: reacting microcrystalline cellulose with vitamin C to consume nitrite in the microcrystalline cellulose so as to prevent the nitrite from reacting with secondary amino in levamlodipine to generate nitrosamine impurities with genetic toxicity;

The invention discloses a composite biological inoculant for promoting alcoholization of crude tobacco, which is prepared by the following steps: by taking bacillus licheniformis CICC 10037, candida utilis CICC 1769 and thermolysis thermophila ATCC 27730 as core functional strains, respectively activating and enlarging culture, then carrying out thallus resuspension by using a stable protective agent solution consisting of trehalose, sodium glutamate and sodium carboxymethyl cellulose, and finally, carrying out fermentation, so as to obtain the composite biological inoculant for promoting alcoholization of crude tobacco. The three strains are mixed according to a specific volume ratio to prepare a compound bacterium suspension, and finally the compound bacterium suspension is uniformly adsorbed to a sterilized homologous tobacco powder carrier, so that macromolecular substances such as starch, protein and cellulose in tobacco leaves can be efficiently degraded through the synergistic effect of the three strains, and the variety and content of aroma components are remarkably increased; the method effectively reduces irritant substances such as ammonia and acetaldehyde and harmful ingredients such as nitrosamine specific to tobacco, reduces offensive odor and irritation, improves taste, ensures uniformity and stability of the alcoholization effect, and achieves multiple purposes of quality improvement, aroma enhancement, harm reduction and efficiency improvement in the crude tobacco alcoholization process.

This invention relates to the preparation and application of a thiazolinone-oxanthracene peroxynitroso fluorescent probe. The probe has the following structural formula: This invention provides a method for synthesizing this fluorescent probe using thiazolinone-oxanthracene dye, pinacol ester of 4-bromomethylphenylboronic acid, etc., as raw materials; this fluorescent probe is a peroxynitroso fluorescent probe with near-infrared emission; firstly, this fluorescent probe is effective against ONOO... ‑ It exhibited good sensitivity, with a 7.5-fold increase in fluorescence intensity; secondly, this fluorescent probe showed good sensitivity to ONOO. ‑ It exhibits high selectivity, unaffected by other reactive oxygen species, biothiols, and amino acids; furthermore, this fluorescent probe is compatible with ONOO. ‑ It acts rapidly, with a response time of less than 5 minutes; in addition, the fluorescent probe is used to detect the content of peroxide nitrosamines in living cells.

An injection molded TPE (Thermoplastic Elastomer) teat (2, 13) based on a styrene block copolymer or a pacifier (1) with a teat (2, 13) having a Shore hardness A of between 30 and 40 is disclosed. A nipple (2, 13) or a pacifier (1) with a nipple (2, 13) comprises a nipple tip material (14) and a nipple edge locking material (15) at the bottom of the nipple (2, 13), intended to fit into a shield (3) and to be locked in position by a grip (4) and a locking portion (17) above a hole (16) on the back of the nipple (2, 13); alternatively, the teat (2, 13) is provided with a shield (3) at the end of the teat (2, 13) and an edge grip (5) on the back (7) of the shield (3), which are integrally formed from TPE. The teat material portion (2, 13) is the thinnest material portion in the range of 0.5 mm to 1.5 mm, and the shield (3) and the grip (4) are thicker than the teat material (14). The TPE nipple or pacifier is a substitute for known natural rubber and silica gel nipples or pacifiers. The TPE material is unvulcanized and thus free of harmful materials, such as nitrosamines and formaldehyde. The pacifier can be repeatedly used. The teat / pacifier has a perfect combination of tear strength and tensile strength and provides bite resistance at 40 A Shore hardness. TPE blends having a Shore hardness of 37.5 A have passed the tests for tear and bite. Since TPS (thermoplastic elastomer based on styrene block copolymer) is lighter, the pacifier / pacifier has reduced weight.

The traditional filters used in cigarettes are found to be ineffective in trapping the toxic chemical compounds which cigarette produces during combustion. The current invention aims to provide systems and methods (or to a universal filter) to reduce amines and nitrosamines in cigarette smoke vapors through a filter functionalized with olive tree polyphenols. Amines and Nitrosamines are highly toxic chemicals in the smoke and aerosol of a combusted cigarette. Olive polyphenols are able to trap amines and nitrosamines by establishing weak and strong interactions with these compounds by trapping these compounds in the filter.

The invention relates to a low-temperature rapidly-vulcanized ethylene propylene diene monomer rubber material, which belongs to the technical field of rubber, 5-vinyl-2-norbornene is used as a third monomer for copolymerization to form VNB type ethylene propylene diene monomer rubber, and hydrogen-containing silicone oil is used for replacing traditional sulfur and peroxide as a vulcanization cross-linking agent of the ethylene propylene diene monomer rubber, so that the low-temperature rapidly-vulcanized ethylene propylene diene monomer rubber material is obtained. The hydrogen-containing silicone oil reduces reaction activation energy through a platinum catalyst and directionally attacks vinyl at the tail end of the VNB type ethylene propylene diene monomer to form a stable Si-C bond, so that disordered cross-linking and free radical side reaction of double bonds in a molecular chain are avoided, low-temperature efficient cross-linking is realized, and the service life of the VNB type ethylene propylene diene monomer is prolonged. Vulcanization byproducts such as nitrosamine, methane and acetone generated in sulfur vulcanization and peroxide vulcanization systems can also be avoided, the crosslinking agent has the advantages that a crosslinking network is uniform and compact, byproducts are not generated in the vulcanization process and the like, and energy consumption and emission in the vulcanization process of rubber products can be effectively reduced.

The invention discloses radiopharmaceutical composition of Fluorine-18 (F18) labelled styrylpyridine compounds, preferably F18 Florbetapir, free of undesirable nitrosamine drug substance related impurities (NDSRI) and other undesirable impurities. The prepared radiopharmaceutical composition is used as a medicament for the treatment or diagnosis of a disease or condition associated with build-up of amyloid-β (Aβ) aggregates.

The application provides a pharmaceutical composition of venlafaxine hydrochloride, which comprises a pill core, a quick-release layer and a slow-release layer, and optionally further comprises an isolation layer and / or a protective layer, wherein the quick-release layer contains an antioxidant. The pharmaceutical composition provided by the application has good stability, can effectively inhibit the growth of nitrosamine impurities in the process of preparation and the process of storage of the preparation, has little influence on related substances, has good storage stability of the product, and has beneficial effects such as similar dissolution of the preparation to a reference preparation. The application further provides a preparation method of the pharmaceutical composition, which is simple in operation and suitable for industrial application.