40 results about "Orbitrap" patented technology

The application discloses a compound qualitative identification system and method based on a dual-mode mass spectrum analysis, which utilizes a quadrupole mass spectrometer and an orbitrap mass spectrometer to synchronously provide spectrum information; taking the spectrum of the quadrupole mass spectrometer as an index, a plurality of matching results are obtained through NIST spectrum library retrieval, each matching result comprises a compound name, a matching degree and a possibility; if the matching degree and the possibility both reach a threshold value, the corresponding compound name is taken as a qualitative result; otherwise, the matching degrees are arranged from high to low, the first N matching results are taken as candidate results, and the theoretical mass numbers of fragment ions of the corresponding candidate compounds are determined respectively; the measured mass numbers are determined according to the spectrum information provided by the orbitrap mass spectrometer; the mass accuracies of the N candidate compounds are calculated respectively; the mass accuracies less than or equal to a threshold value are screened and arranged from low to high, and the candidate compounds corresponding to the first M mass accuracies are reserved as qualitative results.

The application relates to the technical field of pharmaceutical analysis, and discloses a pharmacokinetic analysis method of Tongwei Xiaozheng medicinal liquid, which adopts Q-Orbitrap high-resolution liquid chromatography-mass spectrometry to analyze the concentration of Jasmonic acid in blood components of the Tongwei Xiaozheng medicinal liquid in rat plasma, establishes a plasma concentration determination method for the blood original component Jasmonic acid, carries out methodological verification such as specificity, precision and accuracy, and then calculates pharmacokinetic parameters. In the Tongwei Xiaozheng medicinal liquid, 92 blood components are identified, 17 of which are original components and 75 of which are metabolites, the application firstly determines that Jasmonic acid is one of the blood original components of the Tongwei Xiaozheng medicinal liquid, and reveals the pharmacokinetic characteristics of the Jasmonic acid in normal and model rats. The method has strong specificity, high sensitivity and good reproducibility, and can provide a scientific basis for the research on the pharmacodynamic material basis of the Tongwei Xiaozheng medicinal liquid, the establishment of quality control standards and the clinical rational use of drugs.

The application belongs to the technical field of analytical chemistry, and discloses a qualitative analysis method of ethinyl estradiol and degradation products thereof, which comprises the following steps: determining an accurate mass database and a mass spectrum library of the ethinyl estradiol and degradation products thereof to be detected by using an ultra-high pressure liquid chromatography-quadrupole-electrical field orbitrap high resolution mass spectrometer; detecting the sample by using the ultra-high pressure liquid chromatography-quadrupole-electrical field orbitrap high resolution mass spectrometer; and comparing and analyzing the detection result of the sample with the established accurate mass database and mass spectrum library, so that when the detection result matches the information of the accurate mass database and mass spectrum library, it is determined that the ethinyl estradiol and degradation products thereof to be detected are detected in the sample. The application can realize high-precision qualitative analysis of the sample to be detected by searching for parent ions and daughter ions of the ethinyl estradiol and degradation products thereof by using the ultra-high pressure liquid chromatography-quadrupole-electrical field orbitrap high resolution mass spectrometer, and establishing a high-resolution database.

The application provides a static orbiting trap mass spectrometer with multiple ion sources, which comprises a static orbiting trap and an ion source. The static orbiting trap comprises a conical inner electrode and an outer electrode coaxially sleeved on the outside. The outer electrode is formed by the butt joint of two hollow conical electrode cylinders. The cavity between the outer electrode and the inner electrode constitutes an ion analysis cavity. The ion source is arranged on the side of the static orbiting trap. The ion source is in a cylindrical shape and emits ions along the axis. The ion source comprises repulsion electrodes, an ionization chamber and a lens sleeve which are sequentially arranged along the axis. An incident slot is formed on the outer wall of one of the electrode cylinders. The ion source emits ions into the incident slot, so that the ions enter the ion analysis cavity and move in the same spiral direction. There are at least two ion sources. One of the ion sources emits small mass number ions, and the other emits large mass number ions. The two ion sources are directly coupled with the static orbiting trap, which can weaken the mass discrimination effect, realize the detection and analysis of small mass number ions, and improve the mass range.

The invention discloses a method for screening, identifying and risk evaluating PFAS in surface water and application, and belongs to the technical field of environment monitoring analysis and risk evaluation. The technical problem to be solved is to provide a method with the advantages of high pretreatment efficiency, wide screening range, accurate quantitative analysis, fine risk assessment and the like aiming at the problems of incomplete PFAS screening coverage, inextreme quantitative sensitivity and non-detailed risk assessment in the prior art. According to the technical scheme, the method is characterized in that the pretreatment process of a surface water sample is optimized through full-automatic solid-phase extraction equipment, and comprehensive identification and accurate analysis of traditional and emerging PFAS are achieved in combination with non-targeted screening of Orbitrap Explores 120 high-resolution mass spectrometry and targeted quantification of 7500 triple quadrupole mass spectrometry; and comprehensive evaluation of ecological and health risks is completed through a risk entropy model and a human body multi-tissue cell toxicity experiment.

The invention discloses a mass spectrometric detection method for bisphenol A in milk through rotary evaporation pretreatment and multi-charge peak analysis. The mass spectrometric detection method comprises the following steps: step 1, carrying out rotary evaporation pretreatment on fresh milk; step 2, carrying out rotary evaporation treatment on the milk sample; 3, omics analysis of lipid, metabolites and protein is completed, and UHPLC-Q-Orbitrap HRMS strategy processing based on vDIA is achieved; and 4, analyzing the group database by adopting a multi-charge overlapping peak extraction algorithm and a graph neural network strategy to realize substance identification. The invention belongs to the technical field of mass spectrometry, and solves the problems that the detection error is large when microgram / L-level trace detection of bisphenol A in milk is carried out in the prior art, and false positive is easy to occur due to a wide mass window in the traditional extraction ion chromatography.

The invention relates to the technical field of food detection, and discloses a dregs vinegar fingerprint spectrum construction method and application, the steps are as follows: sample preparation, detection according to specific liquid chromatography-mass spectrometry conditions: Synrgi 4uHydro-RP 80A chromatographic column, acetonitrile-formic acid / phosphoric acid aqueous solution gradient elution, Orbitrap Explorer 120 mass spectrometer and the like. An included angle cosine method, a self-compiled R language program and Python analysis are adopted to establish the dregs vinegar fingerprint spectrum. The fingerprint spectrum of the dross vinegar provided by the invention can comprehensively reflect common chemical components of the dross vinegar, can also be used for quality control, authenticity identification and industrial standardization of the dross vinegar, effectively standardizes the market, and solves the problem of uneven industrial quality.

The invention provides a liquid chromatogram-high resolution mass spectrum combined detection method for perfluorinated compounds in an environment, and belongs to the technical field of compound detection. The method comprises the following steps: pre-treating a to-be-detected water sample, optimizing chromatographic and mass spectrum conditions, and detecting a to-be-detected sample solution by adopting quadrupole rod-electrostatic field orbitrap high-resolution mass spectrum. By optimizing the parameters of the eluent in the chromatography, the matrix interference is effectively removed, the standard addition recovery rate, accuracy and precision of the sample are high, the experimental result is accurate, the error is small, the rapid screening of the target compound can be met, and the detection result is accurate.

A method for component analysis of sauce-aroma Baijiu on the basis of untargeted metabonomics technology, and the use thereof. The analysis method comprises the following steps: (1) sampling, involving: taking a sauce-aroma Baijiu sample as a sample to be subjected to detection, without requiring any sample pretreatment; (2) detection and analysis of said sample on the basis of an ultra-high performance liquid chromatography-quadrupole Orbitrap mass spectrometry technique, wherein after all the components in said sample are separated by means of ultra-high performance liquid chromatography, the components directly enter a mass spectrometer for determination; and (3) qualitative analysis, involving: performing qualitative analysis on the components in said sample on the basis of the data obtained from the determination in step (2), wherein the chromatographic column of the ultra-high performance liquid chromatography is InfinityLab Poroshell HPH-C18 (2.1×100 mm, 1.9 μm). The provided method can achieve more comprehensive and more accurate analysis of components in sauce-aroma Baijiu, and provides a brand-new development direction for detailed analysis of chemical components in Baijiu.

The application discloses a kind of Sabina pollen aerosol chemical component detection and feature database construction method, comprising the following steps: (1) sample processing: collecting Sabina flower, after drying and crushing, extraction is obtained to be measured solution;(2) using the method of ultra-high performance liquid chromatography-quadrupole / or electrostatic field orbitrap high-resolution mass spectrometry, small molecule chemical components in Sabina pollen are systematically separated and identified;(3) data processing: including database establishment, data acquisition and processing, relevant literature search, representative compound selection and cleavage rule derivation.The present application not only lays a theoretical foundation for the rapid screening of Sabina pollen allergenic small molecule markers, the analysis of allergenic mechanisms, and the prevention and control of spring pollen allergy, but also provides a referenceable technical method for the identification of chemical components and mass spectrometry behavior research of similar pollen plants.

The application discloses a filter enrichment detection device, which comprises a filter enrichment device and a detection device. The filter enrichment device comprises a multi-stage filter device (11), an enrichment diffusion resolution device (12) and a suction pump (13). The detection device comprises a single-photon ionization time-of-flight mass spectrometer (21) and an orbitrap mass spectrometer. The application also discloses a detection method for cigarette aerosol by using the device. The device can accurately determine the content of harmful substances and main components in the cigarette aerosol.

The application provides a voltage compensation method, device, equipment and mass spectrometer. The method comprises the following steps: setting a plurality of first voltage combinations for a plurality of adjustable electrodes on an orbitrap structure of a mass spectrometer; determining a first frequency variance based on corresponding electrostatic field distributions of the plurality of first voltage combinations on the plurality of adjustable electrodes; inputting a training data set into a preset prediction model for first iteration optimization to obtain a second voltage combination; inputting the second voltage combination and a second frequency variance corresponding to the second voltage combination into the prediction model for second iteration optimization to obtain a third voltage combination; and performing voltage compensation on each adjustable electrode on the orbitrap structure based on the third voltage combination. In the application, the step of determining the optimal voltage combination is greatly simplified, and the calculation time for determining the compensation voltage is reduced.

The invention relates to the technical field of single cell analysis of cell biology and analytical chemistry, in particular to a flow type single cell scale adjustable device for electrospray ionization mass spectrometry and an adjusting method. According to the present invention, the detection requirements of multi-size cells can be met, the target cells with different sizes in the range of 10-100 [mu] m can be screened through the arched channel switching of the arched shunting capture module, and the comprehensiveness of the detection sample can be ensured. According to the device, the arched channels can be rapidly switched to adapt to cells of different sizes without disassembling the device, continuous operation of sample introduction, screening, extraction, ionization and detection is realized through integrated flow design, high throughput and convenience are taken into account, and the device is adaptive to diversified environmental pollutant exposure metabolism research scenes. The cell suspension and the diluent are mixed on line to reduce non-volatile salt interference, the high-voltage electric field and the sheath gas cooperate to improve ionization efficiency, qualitative and quantitative analysis is achieved through orbitrap mass spectrometry, reliable data support is provided for accumulation, distribution and metabolic transformation characteristics of pollutants in cells, and detection accuracy is guaranteed.

The invention discloses a method for establishing a high-resolution mass spectrum database for screening drugs in food and application thereof, and belongs to the technical field of food analys.The method comprises the following steps that methyl alcohol serves as a solvent to dissolve standard substances of equal-mass target compounds, a single-standard stock solution or a mixed-standard stock solution is obtained, and then a standard solution with the concentration being 1.00 mg / L is prepared; obtaining the retention time, fragment ion information and optimal collision energy of the target compound by using an ultra-high performance liquid chromatography-electrostatic field orbitrap high-resolution liquid chromatograph-mass spectrometer; the method comprises the following steps: accurately importing information of a target compound into TraceFinder software, and constructing a targeted screening acquisition method and a screening database; and calling the database in the TraceFinder software to carry out retrieval, and setting retrieval parameters. According to the method provided by the invention, 400 compounds (including isomeride) can be separated, the detection is more accurate, and the working efficiency of daily inspection and the risk monitoring level of illegal addition in food are greatly improved.

The application provides a device and a control method for improving ion storage and ejection efficiency of an electrostatic field orbitrap mass spectrometer, the device comprising a power supply for applying radio frequency voltage and direct current voltage to electrodes; a plurality of groups of electrodes including a central group of electrodes and a peripheral group of electrodes, the central group of electrodes having an ion exit hole, and the peripheral group of electrodes being symmetrically arranged about the central group of electrodes; each group of electrodes surrounding an ion channel, and the plurality of groups of electrodes being sequentially arranged to form a linear ion channel, the power supply applying radio frequency voltage and direct current voltage to each group of electrodes; the gap J between the nth group of electrodes and the (n+1)th group of electrodes being gradually increased from the central group of electrodes to the peripheral group of electrodes n The application meets the condition and has the advantages of simple structure.

The invention discloses a compound qualitative identification system and method based on dual-mode mass spectrometry. The method comprises the following steps: synchronously providing spectrogram information by using quadrupole rod mass spectrometry and orbitrap mass spectrometry; a spectrogram of the quadrupole mass spectrum is used as an index, a plurality of matching results are obtained through NIST spectrum library retrieval, and each matching result comprises a compound name, a matching degree and possibility; if the matching degree and the possibility both reach the threshold value, taking the name of the corresponding compound as a qualitative result; otherwise, arranging the matching degrees from high to low, taking the first N matching results as candidate results, and respectively determining theoretical mass numbers of fragment ions of corresponding candidate compounds; determining an actually measured mass number according to spectrogram information provided by the orbitrap mass spectrum; calculating the respective quality precision of the N candidate compounds; the quality precision smaller than or equal to the threshold value is screened and arranged from low to high, and candidate compounds corresponding to the first M quality precision are reserved to serve as qualitative results.

The application discloses a kind of based on offline two-dimensional high-speed counter-current chromatography-ultra-high performance liquid chromatography tandem quadrupole electrostatic field orbitrap mass spectrometry-molecular network enhanced chemical composition characterization method;The application first proposes that counter-current chromatography is combined with ultra-high performance liquid chromatography tandem quadrupole electrostatic field orbitrap mass spectrometry and feature-based molecular network, for comprehensively characterizing the chemical components in complex sample (for example: white and root), significantly improve the detection of trace component, realize more comprehensive chemical component characterization.

The invention discloses a zebra fish-based spatial resolution lipidomics method, and belongs to the technical field of biological medicines. The zebrafish-based spatial resolution lipidomics method provided by the invention comprises the following steps: separating a zebrafish trunk from yolk; sample pretreatment and chromatography-mass spectrometry detection. According to the method, yolk is sucked by a pipettor, trunks are cleaned in batches by a vortex instrument, after the trunks and the yolk are pretreated, a sample is subjected to lipidomics detection by an ultra-high performance liquid chromatography tandem quadrupole electrostatic field orbitrap high-resolution mass spectrometer, and further data analysis is performed. According to the method, the trunk and yolk samples which can be used for high-quality and high-coverage lipidomics analysis are obtained while high sample flux is maintained for the first time, and regionalized analysis of zebrafish embryo / larva lipid metabolism is realized. The method has the advantages of simple sample mechanism, low lipid signal interference, high sensitivity and the like, and can detect inrecognizable subtle changes and key lipid molecules in the whole sample.

The invention relates to the field of biological detection, in particular to a method for separating, detecting and identifying flavonoid components in cocoa. The invention provides a method for high-throughput identification of flavonoid substances in cocoa. The method comprises the following steps: 1) extracting the flavonoid substances in the cocoa by using an ultrasonic extraction technology; (2) detecting flavonoid components by using an ultra-high performance liquid chromatography-orbitrap mass spectrometry technology; 3) establishing a standard map of cocoa flavonoid components; and 4) carrying out first-step operation and second-step operation on cocoa to be identified, and then comparing the cocoa with the standard map established in the third step so as to finish the separation and identification of the flavonoid substances. According to the method for identifying cocoa flavonoid substances, the flavonoid substances in cocoa can be rapidly and efficiently extracted and detected, and the influence of isomeride ion interference is reduced.

The invention relates to the technical field of Chinese patent medicine analysis, in particular to a multi-component characterization method of Jichuan decoction and application of the multi-component characterization method. According to the multi-component characterization method provided by the invention, an off-line two-dimensional liquid chromatography tandem quadrupole-electrostatic field orbitrap high-resolution mass spectrometry technology is adopted, and efficient and accurate analysis of various chemical components of Jichuan decoction is realized through specific chromatographic conditions and mass spectrometry conditions. By means of the method, more than two hundred compounds can be identified from the Jichuan decoction, the compounds contain flavonoids, terpenoids, phenylethanoid glycosides, organic acids, coumarins, phenylpropanoids, iridoids, phthalides, steroids and other components, and reference can be provided for research of pharmacodynamic substances of the Jichuan decoction.

The application provides a high-resolution mass spectrometry method for screening Ganoderma lucidum adulterated with Ganoderma amphiacep. The method belongs to the technical field of medicine analysis and comprises the following steps: preparing a test sample solution from a to-be-tested Ganoderma lucidum sample; using an ultra-high performance liquid chromatography-electric field orbitrap high-resolution mass spectrometry technology to detect the test sample solution under a negative ion mode and with 0.1% formic acid-acetonitrile gradient elution; extracting characteristic ions with mass-to-charge ratios of 357.17 and 599.32 from a total ion chromatogram obtained through detection; and determining that the to-be-tested Ganoderma lucidum sample contains Ganoderma amphiacep if a chromatographic peak with a mass-to-charge ratio of 357.17 and two isomer chromatographic peaks with a mass-to-charge ratio of 599.32 are detected simultaneously. The application uses high-resolution mass spectrometry to screen specific characteristic compounds of Ganoderma amphiacep, establishes a unified screening method suitable for medicinal materials, decoction pieces and Chinese patent medicines, and solves the problem that the prior art cannot distinguish Ganoderma amphiacep from genuine Ganoderma lucidum.

The invention provides a non-target analysis method for combustion heat released organic components based on multi-platform chromatography-mass spectrometry. According to the method, a sample decomposition characteristic temperature interval is determined through thermogravimetric analysis, a simulated thermal treatment experimental platform is constructed, and comprehensive coverage detection of volatile, semi-volatile and polar organic components is realized based on a gas chromatography-quadrupole time-of-flight high-resolution mass spectrum (GC / QTOF-HRMS) and a high performance liquid chromatography-orbitrap high-resolution mass spectrum (LC / Orbitrap-HRMS). According to the method, the compound confidence level is judged by fusing multi-platform non-target grading credibility, the identification accuracy is improved by using an adduct ion and fragment information complementation mode, and compound classification, statistics, detection rate calculation, structural formula generation and feature integration are realized by combining a small automatic data processing algorithm based on rules. According to the method, the coverage range and efficiency of a non-target screening strategy can be remarkably improved.

The application belongs to the field of traditional Chinese medicine preparation analysis, and specifically discloses a method for rapidly identifying main chemical components in Guipi mixture by using ultra-high performance liquid chromatography-quadrupole-electric field orbitrap high-resolution mass spectrometry (UHPLC-Q-Exactive MS) technology, which realizes the purpose of rapidly qualitatively identifying 115 components such as phenylalanine, phthalide, flavonoids, ketones and terpenes in Guipi mixture. The method has the advantages of simplicity, rapidness, high efficiency and sensitivity, and can provide evidence for establishing the interaction between the components and the curative effect of Guipi mixture, and provide basis for the research on the safety and effectiveness of clinical medication.

The application discloses a method for non-target identification and characterization of non-volatile organic compounds in a mask, comprising the following steps: (1) sample pretreatment; (2) performing ultra-high performance liquid chromatography separation and quadrupole-electrostatic field orbitrap high-resolution mass spectrometry analysis on the sample; and (3) data processing and analysis. The application characterizes the detected compounds in different types of masks by adopting hierarchical cluster analysis and principal component analysis methods, and can provide valuable references for monitoring of new non-volatile organic chemicals and improvement of mask safety production measures.

The invention provides a method for detecting lung cancer markers in exhaled gas based on mass spectrum combined with a sampling device, and relates to the field of analytical chemistry detection, and the method comprises the following steps: 1, collecting the exhaled gas of a patient by using the sampling device; and step 2, Orbitrap Fusion-EESI MS detection: adopting an EESI source and an Orbitrap Fusion high-resolution mass spectrometer to detect the collected exhaled gas in a positive ion mode. The detection parameters in the step 2 are as follows: the ionization voltage is 3KV, the sheath gas is 50-70Arb, the temperature of an ion transfer tube is 320 DEG C, the flow rate of an ionization reagent is 5-20 [mu] l / min, the flow rate of a sample is 5-20 [mu] l / min, and the first-stage mass spectrum scanning mass number range is 50-750. According to the method, the markers in the exhaled gas of the lung cancer patient are qualitatively analyzed by adopting an Orbitrap Fusion-EESI MS detection method assisted by capturing the exhaled gas through an extracting agent, the method has the advantages of one-time sample injection, effective analysis, real time and rapidness of the EESI MS detection method, and meanwhile, the sensitivity of Orbitrap Fusion high-resolution mass spectrum is enhanced through auxiliary detection of an ionization reagent, so that the detection accuracy of the lung cancer patient is improved. And the detection effect of the effective components in the exhaled gas is improved.

The invention relates to the technical field of detection, and particularly discloses a high-throughput screening and quantifying method for anesthetics in aquatic products based on high-resolution mass spectrometry, which comprises the following steps: constructing a mass spectrometry database containing information of at least 72 anesthetic compounds; the method comprises the following steps: carrying out pretreatment on an aquatic product sample by adopting an improved QuEChERS method; carrying out analysis and acquisition under specific chromatography and mass spectrometry conditions by utilizing an ultra-high performance liquid chromatography-electrostatic field orbitrap high-resolution mass spectrometer; the method comprises the following steps: acquiring data by adopting a Full MS-ddMS2 scanning mode; and finally, analyzing the data through a'non-targeted discovery-targeted confirmation 'strategy, namely, firstly performing non-targeted screening by using Compressed Discovery software, and then performing targeted confirmation and quantification on the basis of the mass spectrum database through TraceFinder software, the method is high in flux, good in sensitivity and strong in anti-interference capability, and the method is suitable for large-scale popularization and application. Various types of anesthetic residues in aquatic products can be rapidly and accurately screened, identified and quantified, and the defects of an existing standard detection method are overcome.

The invention discloses a UPLC (Ultra Performance Liquid Chromatography)-Q-Orbitrap (Quantitative Orbitrap) screening method for multi-structure type new pollutants in sediments or soil. The method comprises the following steps: firstly, determining UPLC-Q-Orbitrap-HRMS instrument analysis conditions aiming at the multi-structure type new pollutants, optimizing a pretreatment method of a sample, then constructing a mass spectrum database of the multi-structure type new pollutants, carrying out analysis and data acquisition on an actual sediment or soil sample on the basis of the determined UPLC-Q-Orbitrap-HRMS instrument analysis conditions and the pretreatment method, and finally determining a mass spectrum of the multi-structure type new pollutants. And determining the matched multi-structure type new pollutants by using the determined screening standard. Based on the UPLC-Q-Orbitrap-HRMS technology, a broad-spectrum analysis method for qualitative screening before quantification is established, and the method is based on the high-resolution mass spectrum, adopts a unified instrument analysis and extraction process, and can be used for rapidly identifying various types of new pollutants with different structures in environmental sediments and soil at the same time.

The invention relates to a solid-phase extraction material for detecting caine anesthetics in livestock and aquatic products as well as a preparation method and application of the solid-phase extraction material, and belongs to the technical field of solid-phase extraction. The preparation method of the solid-phase extraction material for detecting the caine anesthetics in the livestock and aquatic products comprises the following steps: S1, preparing St-DVB copolymer microspheres; s2, preparing a sulfonic group functionalized St-DVB copolymer microsphere; s3, preparing a sulfonic acid group / C18 bifunctional functionalized St-DVB copolymer, and filling the sulfonic acid group / C18 bifunctional functionalized St-DVB copolymer into a solid-phase extraction column; and S4, accurately weighing the prepared livestock and aquatic products in a centrifuge tube, adding an acetic acid acetonitrile solution for extraction and centrifugation, transferring all the supernatant to a solid-phase extraction column, carrying out elution and elution, collecting the target object, carrying out nitrogen blowing concentration and redissolution, filtering through a membrane, and carrying out ultra-high performance liquid chromatography-electrostatic field orbitrap high-resolution mass spectrometry determination. According to the application, accurate detection of the caine anesthetics in livestock and aquatic products is successfully realized, and a promising technical scheme is provided for daily supervision and quality control of the caine anesthetics.

Systems and methods for specialized data acquisition in capillary electrophoresis electrospray ionization mass spectrometry (MS) that includes (a) mass-to-charge (m / z) vs. migration time (MT) correlation, (b) ion mobility (IM) vs. MT correlation, and (c) m / z vs. IM vs. MT correlation to advance molecular analysis via data-dependent, data-independent, and targeted analysis methods executed on mass spectrometers using diverse types of mass analyzers, including but not limited to orbitrap, time-of-flight, and ion mobility time-of-flight mass analyzers. Electrophoresis-correlative (Eco) MS enhances the detection, identification, and quantification of molecules, as is demonstrated here for complex proteome samples.

The invention provides a method for measuring the residual quantity of glyphosate, glufosinate-ammonium and aminomethylphosphonic acid in tea leaves, and belongs to the technical field of analysis and testing. The method comprises the following steps: 1) adding an internal standard substance into a tea sample to be detected, adding water, performing ultrasonic extraction and low-temperature centrifugation, and taking supernate A; step 2), adding a purifying agent which at least comprises octadecylsilane chemically bonded silica into the supernate A, performing vortex oscillation and low-temperature centrifugation, and taking supernate B and a filter membrane for liquid chromatography-electrostatic field orbitrap mass spectrometry. The method for detecting the content of the glyphosate and the glufosinate-ammonium in the tea leaves is rapid and accurate, the relative average deviation is smaller than 5.0%, the reliable and convenient-to-implement method is provided for detecting the content of the glyphosate and the glufosinate-ammonium in the tea leaves, and the requirement for detecting the glyphosate and the glufosinate-ammonium in the tea leaves can be met.