5 results about "Orbitrap" patented technology

The application discloses a kind of Sabina pollen aerosol chemical component detection and feature database construction method, comprising the following steps: (1) sample processing: collecting Sabina flower, after drying and crushing, extraction is obtained to be measured solution;(2) using the method of ultra-high performance liquid chromatography-quadrupole / or electrostatic field orbitrap high-resolution mass spectrometry, small molecule chemical components in Sabina pollen are systematically separated and identified;(3) data processing: including database establishment, data acquisition and processing, relevant literature search, representative compound selection and cleavage rule derivation.The present application not only lays a theoretical foundation for the rapid screening of Sabina pollen allergenic small molecule markers, the analysis of allergenic mechanisms, and the prevention and control of spring pollen allergy, but also provides a referenceable technical method for the identification of chemical components and mass spectrometry behavior research of similar pollen plants.

The application provides a voltage compensation method, device, equipment and mass spectrometer. The method comprises the following steps: setting a plurality of first voltage combinations for a plurality of adjustable electrodes on an orbitrap structure of a mass spectrometer; determining a first frequency variance based on corresponding electrostatic field distributions of the plurality of first voltage combinations on the plurality of adjustable electrodes; inputting a training data set into a preset prediction model for first iteration optimization to obtain a second voltage combination; inputting the second voltage combination and a second frequency variance corresponding to the second voltage combination into the prediction model for second iteration optimization to obtain a third voltage combination; and performing voltage compensation on each adjustable electrode on the orbitrap structure based on the third voltage combination. In the application, the step of determining the optimal voltage combination is greatly simplified, and the calculation time for determining the compensation voltage is reduced.

The application discloses a kind of based on offline two-dimensional high-speed counter-current chromatography-ultra-high performance liquid chromatography tandem quadrupole electrostatic field orbitrap mass spectrometry-molecular network enhanced chemical composition characterization method;The application first proposes that counter-current chromatography is combined with ultra-high performance liquid chromatography tandem quadrupole electrostatic field orbitrap mass spectrometry and feature-based molecular network, for comprehensively characterizing the chemical components in complex sample (for example: white and root), significantly improve the detection of trace component, realize more comprehensive chemical component characterization.

The application discloses a method for detecting chemical components in Artemisia annua pollen aerosol and constructing a characteristic database, which comprises the following steps: (1) sample treatment: collecting Artemisia annua pollen aerosol, storing in a low temperature and sealing, extracting after drying and crushing to obtain a solution to be tested; (2) performing chromatography and mass spectrometry analysis on the solution to be tested; (3) data processing: including database establishment, data collection and processing, relevant literature searching, representative compound selection and cleavage rule derivation. The application adopts UHPLC-Q-Orbitrap HRMS technology, systematically separates and identifies chemical components in Artemisia annua pollen, focuses on analyzing mass spectrometry cleavage characteristics of main category compounds such as sesquiterpenes, flavones and phenylpropanoids, summarizes the cleavage rule, fills the blank of research on small molecule chemical components and mass spectrometry cleavage of Artemisia annua pollen, and provides a new thought and scientific basis for quality evaluation, sensitization mechanism analysis and resource comprehensive utilization of Artemisia annua pollen.

The application relates to the field of biotechnology, and particularly relates to a method for obtaining a biological marker of a buffalo estrus period and application of the biological marker to identifying the buffalo estrus period, S1, collecting milk samples of a female buffalo, and observing follicle development of the female buffalo by using B ultrasonic waves; S2, after the milk samples are extracted, analysis is conducted by using a quadrupole-electric field orbitrap high-resolution mass spectrometry system and an ultrafast high-performance liquid chromatography system; S3, after original data are converted into an mzXML format, extraction data are obtained by using XCMS software processing, the extraction data are processed and analyzed, and significant difference metabolites of cow milk in an estrus period are screened out; S4, expression amounts of the significant difference metabolites of the cow milk in the estrus period and a non-estrus period are detected, the expression amount changes are compared with B ultrasonic wave results, and a biological marker of the buffalo estrus period is obtained. Compared with the prior art, pyridoxamine and homovanillic acid are obtained as the biological marker of the estrus period of the female buffalo, and are used for identifying whether the female buffalo is in the estrus period.