A method for detecting secondary metabolites in fresh tobacco leaves by using a
derivatization GC-MS (
Gas Chromatography-
Mass Spectrometer) is characterized by comprising the steps: quickly collecting new tobacco leaves and quickly freezing the new tobacco leaves by using
liquid nitrogen on the site, crushing the tobacco leaves after removing the
moisture in the tobacco leaves by means of
freeze drying, extracting the secondary metabolites from tobacco
powder by using a
solvent,
drying the extracted liquid supernatant, then performing oximation reaction and
silane derivatization on the dried extractive, and finally performing analysis by the GC-MS. The method has the prominent advantages that firstly, a method suitable for extracting the secondary metabolites in the fresh tobacco leaves is developed. As the area of the tobacco leaves is larger, the secondary metabolites are not uniformly distributed in the whole tobacco leaves, and the tobacco leaves are not applicable to the
punching sampling technique. However, according to a tobacco leave collection and extraction method provided by the invention, the
collection period and the
metabolite composition of the tobacco leaves at the collected part can be truly reflected. Secondly, most of the important metabolites of the tobacco leaves such as saccharides, amino acids, organic acids,
terpene alcohols in the fresh tobacco leaves can be simultaneously detected, and the method has the characteristics of high
throughput detection.