7 results about "Avidin" patented technology

This work proposes a novel enzyme-free electrochemiluminescence sensing platform based on novel DNA-quantum dot nanostructures and hybridization chain reaction (HCR) amplification, and applies it to trace Cd. 2+ The detection; first, Cd 2+ Aptamer-triggered HCR amplification introduces a large amount of biotin-labeled DNA into the electrode, whereby biotin specifically captures a large amount of avidin SA-CdS quantum dot complexes, exhibiting a high ECL signal; target Cd 2+ Upon binding to its aptamer, the quantum dot-DNA structure detaches from the electrode, resulting in a significant decrease in the ECL signal, thus enabling the control of Cd. 2+ This sensor exhibits ultrasensitive detection with a dynamic response range from 10 fM to 10 nM and a detection limit of 2.6 fM. It demonstrates excellent selectivity, speed, high sensitivity, and practicality for detecting real water samples, providing a novel and competitive strategy for detecting heavy metal ions in real-world samples.

The application relates to a protein sample processing method, comprising the following steps: mixing a protein sample with an alkylating agent to seal free cysteine, obtaining a sealed sample; centrifuging the sealed sample through an ultrafiltration membrane, obtaining a membrane sample; the pore size of the ultrafiltration membrane ranges from 10 kDa to 30 kDa; performing first reduction on the membrane sample, obtaining a first reduced sample; adding a biotin labeling agent to the first reduced sample, obtaining a labeled sample; enzymatically digesting the labeled sample, obtaining an enzymatically digested sample; adding avidin microbead agarose to the enzymatically digested sample to adsorb biotin-labeled proteins, obtaining an enriched sample; performing reduction and alkylization on the enriched sample, obtaining a processed sample.

The application discloses a platelet antibody detection and cross matching blocking chromatography kit and a detection method thereof, wherein the kit comprises a U-shaped microwell plate, a chromatography medium for separating platelet immune complexes from non-cell particle components under the action of centrifugal force, freeze-dried platelets, a platelet diluent, a platelet fluorescent staining solution comprising fluoresceinized platelet antibodies specifically labeled for platelets, biotin-labeled anti-human globulin for specifically binding with antibodies bound to the surface of the platelets, avidinized fluorescein for coupling with the biotin-labeled anti-human globulin through a biotin-avidin specific binding system, negative control serum / plasma and positive control serum / plasma. The application combines the blocking chromatography technology with a high-sensitivity flow immunofluorescence method to construct an efficient and accurate platelet antibody detection and cross matching system, which is high in specificity and sensitivity, simple in operation and stable in detection results.

This invention provides an electrochemical sensor for detecting PDGF-BB using a poly A-DNA tetrahedral probe and its application. The electrochemical sensor is a base-stack-dependent electrochemical sensor, comprising a poly A-tetrahedral probe self-assembled on a gold electrode, aptamer DNA, horseradish peroxidase, and a chromogenic substrate. The aptamer DNA is biotin-modified at its tail, and the horseradish peroxidase is avidin-modified. The electrochemical sensor detects PDGF-BB. The aptamer DNA is triggered by PDGF-BB to transform into a stem-loop structure and stacks with the poly A-tetrahedral probe. Based on the base stacking effect, the aptamer DNA stably hybridizes with the target complementary sequence of the poly A-tetrahedral probe immobilized on the gold electrode. The aptamer DNA and horseradish peroxidase bind via biotin-avidin. The chromogenic substrate is catalyzed to generate an electrochemical redox catalytic signal, which is then output.

This application relates to a method and its application for improving the detection signal value of photo-induced chemiluminescence (PET). The method includes: mixing a companion reagent with antibody-coated luminescent microparticles, antibody-coated biotin, avidin-coated photosensitive microparticles, and a sample to be tested, reacting the mixture to generate a test mixture; exciting the test mixture at least once with excitation light, and measuring the amount of emitted photons to obtain a light signal value; wherein the companion reagent includes an oxygen-generating agent and a catalyst, the oxygen-generating agent undergoes a catalytic reaction under the action of the catalyst to generate oxygen, and the oxygen provides singlet oxygen for PET detection. The solution provided in this application can improve the detection signal value of PET, thereby enhancing the stability, sensitivity, and precision of the detection.

This invention, entitled "An Improved Compatibility Post-Amplification Chemiluminescence Detection System and Its Applications and Products," belongs to the field of in vitro diagnostic technology. It aims to solve technical problems existing in current post-amplification chemiluminescence detection systems, such as insufficient signal amplification, high background signal, poor hardware compatibility, and easy distortion of antigens / antibodies. The post-amplification chemiluminescence detection system of this invention includes a magnetic bead working solution and a labeled working solution; the labeled working solution is composed of a biotinylated labeled carrier, a biotinylated targeting molecule, unmodified avidin, and unmodified biotin, with a molar ratio of (2.5-3.5):1.0:(1.0-2.0):4.0. This invention can achieve efficient multi-cascade signal amplification, reduce background signal and non-specific adsorption, avoid antigen / antibody distortion, is compatible with existing in vitro diagnostic hardware, significantly improves detection sensitivity and signal-to-noise ratio, and has strong universality, applicable to the detection of various antigens and antibodies.

Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.