97 results about "Murine antibody" patented technology

The invention provides a group of monoclonal antibody pairs targeting adenovirus DBP protein, the monoclonal antibody pairs comprise a capture antibody and a detection antibody, each of the two antibodies comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises heavy chain complementarity determining regions HCDR1, HCDR2 and HCDR3, the light chain variable region comprises light chain complementarity determining regions LCDR1, LCDR2 and LCDR3, and the capture antibody and the detection antibody are used for capturing and detecting the adenovirus DBP protein. Wherein the capture antibody is a monoclonal antibody 19E3; and the detection antibody is selected from a monoclonal antibody 96 to 10D8, a monoclonal antibody 95 to 2B7, a monoclonal antibody 96 to 2C10, a monoclonal antibody 96 to 8E10, a monoclonal antibody 96 to 13D5 or a monoclonal antibody 96 to 2B10. The monoclonal antibody pair disclosed by the invention can be used for efficiently detecting various types of HAdV DBP.

The present disclosure relates to antibodies and antibody fragments specific for CD45. The antibodies are improved humanized versions of murine antibodies. In addition to complex humanization activities, the antibodies are engineered to remove multiple detrimental motifs within the CDR region without losing any beneficial properties. The antibodies are useful for the treatment of diseases associated with CD45.

The invention provides a B7H7 targeting antibody or an antigen binding fragment thereof and application thereof. The antibody or the antigen binding fragment thereof comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region CDR sequence has an amino acid sequence as shown in SEQ ID NO: 1-5 or an amino acid sequence with at least 80% of identity with the amino acid sequence as shown in SEQ ID NO: 1-5; the light chain variable region CDR sequence has an amino acid sequence as shown in SEQ ID NO: 6-8 or an amino acid sequence with at least 80% identity with the amino acid sequence as shown in SEQ ID NO: 6-8. The mouse antibody and the humanized antibody prepared by the invention can be specifically combined with B7H7 in a targeting manner, block the combination of B7H7 and a receptor KIR3DL3 thereof, and promote the killing of immune cells on tumor cells, so that B7H7-mediated related diseases are effectively treated or prevented.

The invention provides a time-resolved fluorescence immunochromatography kit and a detection method for simultaneously detecting myocarine and ergodiethylamine. The kit mainly comprises a time-resolved fluorescent microsphere labeled antibody probe and a test strip, carrying out chemical coupling on the time-resolved fluorescent microspheres and a monoclonal antibody of the myocarine and the ergodiethylamine to prepare a time-resolved fluorescent microsphere labeled antibody probe; the test strip comprises a nitrocellulose membrane, a sample pad and an absorption pad which are arranged on a bottom plate, and the nitrocellulose membrane is respectively coated with detection lines T1 and T2 of a myocarine antigen and an ergodiethylamine antigen, and is coated with a quality control line C of a goat anti-mouse IgG antibody. The method can be applied to rapid screening of the maccarine and the ergodiethylamine in the hair sample, is simple to operate, rapid and convenient, accurate in quantification and high in sensitivity, and is suitable for various temporary detection scenes such as community drug rehabilitation, entertainment venues and public security checkpoints.

The utility model discloses a fluorescent quantitative rapid detection card for ofloxacin quantum dot microspheres. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card comprises a test strip, the test strip comprises a bottom plate, a sample pad, a fluorescent microsphere conjugate pad, a nitrocellulose membrane and a water absorption pad, the sample pad, the fluorescent microsphere conjugate pad, the nitrocellulose membrane and the water absorption pad are arranged on the bottom plate and connected in sequence, and detection lines and quality control lines which are distributed at intervals are arranged on the nitrocellulose membrane. The detection line is close to the fluorescent microsphere conjugate pad, the quality control line is close to the water absorption pad, the detection line is coated with a micromolecule ofloxacin antigen, the quality control line is coated with a goat anti-mouse IgG antibody, and a microsphere labeled antibody conjugate in the fluorescent microsphere conjugate pad is a fluorescent microsphere labeled ofloxacin antibody. The method is simple and rapid to operate, is less affected by environment temperature and solvent reagents, is suitable for rapid detection of food safety, and is accurate in detection result.

The invention belongs to the technical field of biology. The invention provides a recombinant protein, an amino acid sequence of the recombinant protein is formed by repeatedly connecting dominant epitopes of canine parvovirus VP2 protein in series, the amino acid sequence of the canine parvovirus recombinant protein is converted into a corresponding nucleotide sequence by adopting escherichia coli preferred codons, the nucleotide sequence is chemically synthesized, and a recombinant expression vector is constructed; therefore, the expression quantity of the recombinant protein in escherichia coli is improved. The invention also relates to a method for establishing a bacteriophage library by immunizing a mouse with the recombinant protein, obtaining a corresponding canine parvovirus VP2 protein single-chain antibody scfv sequence through panning screening, constructing a complete mouse IgG1 antibody sequence expression vector from the obtained scfv sequence, expressing a monoclonal antibody through transient HEK293F cells, and purifying the monoclonal antibody, thereby obtaining the canine parvovirus VP2 protein single-chain antibody. An optimal monoclonal antibody pairing combination is determined through an immunofluorescence orthogonal experiment.

The invention provides a rabbit monoclonal antibody for resisting human NPTXR protein, and belongs to the technical field of biological detection and biological engineering. The rabbit source monoclonal antibody for resisting the human NPTXR protein comprises HZK26-B0005, HZK26-B0006, HZK26-B0007, HZK26-B0008, HZK26-B00010, HZK26-B00012, HZK26-B00013, HZK26-B0014, HZK26-B0016, HZK26-B0017, HZK26-B0026 and HZK26-B0027, and the rabbit source monoclonal antibody for resisting the human NPTXR protein comprises HZK26-B0007, HZK26-B0007, HZK26-B0008, the monoclonal antibody provided by the invention can highly specifically recognize the NPTXR protein, and compared with a mouse or rat antibody, the rabbit monoclonal antibody is more easily humanized due to the characteristics of larger genetic diversity, simple immunoglobulin structure, high affinity and the like.

The present invention provides caninized rat antibodies to canine IL-31 receptor alpha that have a high binding affinity for canine IL-31 receptor alpha, and that can block the binding of canine IL-31 to canine IL-31 receptor alpha. The present invention further provides the use of the antibodies for the treatment of atopic dermatitis in dogs.

The invention discloses a preparation method and application of a clothianidin fluorescent test strip, belongs to the technical field of agricultural detection, and solves the problems that the existing bombyx mori pesticide poisoning detection sensitivity is low, and the clothianidin residue concentration cannot be quickly judged. According to the method, a nitrocellulose membrane is prepared, a detection line is coated with a clothianidin-BSA antigen, and a quality control line is coated with an anti-mouse IgG antibody; when the fluorescent pad is prepared, carboxylated fluorescent microspheres and an anti-clothianidin antibody are coupled to form a labeled probe; when the test strip is assembled, the nitrocellulose membrane, the fluorescent pad, the sample pad and the absorbent paper are stacked and fixed on the bottom plate to form a chromatography structure. Clothianidin in a sample is combined with the labeled probe to form an immune complex, chromatography migrates to the detection line to be competitively combined with clothianidin-BSA antigen, the uncombined probe is captured and develops color, and the quality control line develops color through an anti-mouse IgG antibody. The test strip is used for rapidly detecting clothianidin residues in mulberry leaves and bombyx mori bodies, the sensitivity reaches 0.02 mg / L, pesticide poisoning events can be effectively prevented, and safe production of the sericulture industry is guaranteed.

The invention relates to the field of biological detection, and particularly discloses chromatographic test paper for detecting fingertip blood VEGF as well as a preparation method and application of the chromatographic test paper. The chromatographic test paper comprises a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad which are sequentially lapped, the combination pad is coated with modified sporopollenin microspheres, and the surfaces of the modified sporopollenin microspheres are marked with anti-VEGF monoclonal antibodies and rabbit anti-mouse antibodies. Natural sporopollen is modified, the surface of the modified sporopollen is sequentially coated with a SiO2 shell layer and a hydroxyapatite nanocrystalline mineralization layer, and the sensitivity and stability of the chromatographic test paper on VEGF detection are further improved by utilizing the super-strong physical and chemical stability, the rich hierarchical porous structure and the excellent biocompatibility and mechanical strength of the modified sporopollen.

The present invention relates to a monoclonal mouse IgG antibody against human glycoprotein Ib (GPIb) for therapeutic use for inhibiting a GPIb-mediated activation of platelets, the monoclonal mouse IgG antibody comprising the amino acid consensus sequence tyrosine-valine-methionine (YVM) within the CDR 3 region of the variable domain of the heavy chain.

The application discloses a single-chain antibody, the amino acid sequence of which is shown as SEQ ID NO:1, and the nucleotide sequence of which is shown as SEQ ID NO:2. The single-chain antibody against rabies virus is screened from an antibody library of immunized mice by using a phage display technology. Compared with a traditional cell fusion technology, the phage display technology is simple in operation, low in cost, short in time, and high in antibody discovery and screening efficiency. The single-chain antibody obtained by the application is low in molecular weight and immunogenicity, high in antibody specificity and affinity, and has the activity of neutralizing rabies virus, thereby providing a new idea for basic research of rabies virus and development of therapeutic drugs.

Disclosed are an antibody capable of binding to thymic stromal lymphopoietin and the use thereof. Disclosed are an anti-TSLP antibody, comprising a murine antibody, chimeric antibody and humanized antibody of the light chain and heavy chain variable regions of the anti-TSLP antibody and antigen-binding fragments thereof, or a pharmaceutically acceptable salt or solvent compound thereof, and the use thereof as a drug for treating asthma, especially the use thereof in the preparation of a drug for treating TSLP-positive diseases or conditions.

The invention provides a monoclonal antibody targeting adenovirus DBP protein, the monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises heavy chain complementary determining regions HCDR1, HCDR2 and HCDR3, the light chain variable region comprises light chain complementary determining regions LCDR1, LCDR2 and LCDR3, amino acid sequences of the HCDR1, the HCDR2 and the HCDR3 are respectively shown as SEQ ID NOs: 1-3, and amino acid sequences of the LCDR1, the LCDR2 and the LCDR3 are respectively shown as SEQ ID NOs: 4-6. The monoclonal antibody provided by the invention can be used for efficiently detecting various types of HAdV DBP.

The invention provides a D-dimer detection kit and a preparation method thereof. A chromatographic test strip of the kit comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad. And the combination pad is coated with a D-dimer detection antibody marked by carboxylated time-resolved fluorescent microspheres. A detection line of the nitrocellulose membrane is coated with a D-dimer capture antibody, and a quality control line of the nitrocellulose membrane is coated with a goat anti-mouse IgG antibody. The D-dimer detection antibody and the D-dimer capture antibody are a pair of monoclonal antibodies for specifically recognizing different epitopes of the D-dimer. According to the kit, the convenience of immunochromatography and the high sensitivity characteristic of the time-resolved fluorescent microspheres are fused, accurate and quantitative detection of the D-dimer is achieved through the synergistic effect of the detection antibody and the capture antibody which specifically recognize different epitopes, and the kit is high in sensitivity, good in specificity, easy and convenient to operate, high in anti-interference capacity and high in sensitivity. The clinical high-precision quantitative monitoring requirement can be met, and the diagnosis and treatment application value and the market prospect are achieved.

The invention belongs to the technical field of immunodetection, and particularly relates to a kit and a system for detecting a tumor marker through air pressure based on lateral immunochromatography and application of the kit and the system in detecting the tumor marker. The kit comprises an immune probe with a function of catalyzing hydrogen desorption of a hydrogen-containing compound and an immunochromatography test strip, the immune probe is obtained by coupling an antibody for specifically recognizing a tumor marker to the surface of a nanoparticle; the immunochromatography test strip comprises a bottom plate, and a glass cellulose membrane, a reaction membrane and a water absorption pad which are sequentially connected on the bottom plate along the chromatography direction, wherein a detection line and a quality control line are sequentially arranged on the reaction membrane along the chromatography direction; the detection line is coated with a tumor marker coating antibody, and the quality control line is coated with a goat anti-mouse IgG antibody. According to the kit disclosed by the invention, the content of the tumor marker in trace blood can be rapidly, accurately and quantitatively detected by virtue of the rapid detection advantage of an immunochromatography technology and objective air pressure intensity change.

The present invention relates to monoclonal anti-human-(IDF-15 antibodies. The antibodies include chimeric antibodies and humanized antibodies. The invention also relates to monoclonal anti-human-GDF-15 antibodies including murine antibodies, chimeric antibodies and humanized antibodies for use in methods for the treatment of cancer cachexia and also for the treatment of cancer. The invention also provides pharmaceutical compositions, kits, methods and uses and cell lines capable of producing the monoclonal antibodies of the invention.

The application relates to an AI-assisted result interpretation micro-sample detection kit for cutaneous leishmaniasis infection and an antibody detection method, and belongs to the technical field of serological immunodetection. The kit comprises an antibody detection platform for micro-samples, micro-bead probes, a washing solution, an antibody diluent, F594-labeled sheep anti-rat antibodies, positive quality control serum and negative quality control serum; the spherical probes are adopted in the application, the antigen-antibody combination efficiency is improved in space, and the antigen-antibody collision probability is effectively improved. Meanwhile, the micro-beads form a micro-scale mass transfer network in the reaction cavity, fluid mixing is enhanced, the reaction time is shortened, and the overall residence time and consumption of samples and reagents in the microfluidic channel are reduced. The application also introduces an AI-assisted result interpretation system to assist in result interpretation, significantly improves the friendliness and accessibility of the detection process, the classification model based on artificial intelligence has stronger robustness and judgment consistency, and has good market development value and popularization and application prospect.

The present application belongs to the technical field of bioengineering. The present application relates to a recombinant protein, which comprises two dominant antigen epitopes of feline calicivirus (FCV) protein. In order to improve the yield of the recombinant protein in a prokaryotic expression system, the amino acid sequence of the recombinant protein is converted into a corresponding nucleotide sequence by using E. coli preferred codons, the nucleotide sequence is chemically synthesized, and a recombinant expression vector is constructed. The present application also relates to the establishment of a phage library by immunizing mice with the recombinant protein, the screening of a single-chain antibody (scfv) sequence corresponding to the feline calicivirus recombinant protein by panning, the construction of a complete mouse IgG1 antibody sequence expression vector from the obtained scfv sequence, the expression of a monoclonal antibody by instantaneously transferring HEK293F cells, the purification of the monoclonal antibody, and the labeling of europium ions (Eu 3+ ) in the monoclonal antibody, respectively. The optimal monoclonal antibody pairing combination is determined by orthogonal experiments, and the monoclonal antibody can be used for the early diagnosis of upper respiratory tract infection in cats.

The present disclosure relates to anti-Claudin 18.2 antibodies and uses thereof. In particular, the present disclosure relates to an anti-Claudin 18.2 antibody; murine antibodies, chimeric antibodies, humanized antibodies and antigen-binding fragments thereof comprising the CDRs of the anti-Claudin 18.2 antibody, and uses thereof as a medicament. In particular, the present disclosure relates to the use of an anti-Claudin 18.2 antibody in the manufacture of a medicament for treating a Claudin 18.2 positive disease or disorder.

This disclosure provides anti-B7-H3 antibodies, including murine antibodies, humanized antibodies, and human antibodies, as well as those with further optimized CDR sequences. These antibodies are suitable for use in treating diseases such as cancer.

Provided are an anti-human B7-H3 monoclonal antibody and an application thereof. By using a recombinant human B7-H3 extracellular region as an immunogen, a murine anti-human B7-H3 monoclonal antibody can be prepared by means of a hybridoma technology, and a murine anti-human B7-H3 antibody can bind to various domains of the B7-H3 extracellular region. A human-mouse chimeric antibody constructed on the basis of a murine antibody can specifically bind to B7-H3 of a cell surface. A humanized antibody prepared by means of CDRs transplantation and CDRs region mutation retains the ability to specifically bind to the human B7-H3 extracellular region and cell membrane surface B7-H3, and can be mediated for internalization by the cell membrane surface B7-H3.

The invention discloses a glycosylated hemoglobin detection kit with high reaction rate and preparation, the glycosylated hemoglobin detection kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises blank polystyrene latex microspheres and a reaction buffer system, the reaction buffer system comprises 0.5 g / L-10 g / L of a buffer solution, 1 g / L-5 g / L of a surfactant and 3 g / L-15 g / L of inorganic metal salt; the reagent R2 comprises a compound oligomeric antibody with the concentration of 0.2 g / L to 1.0 g / L and an antibody preservation solution, and the antibody preservation solution comprises a buffer solution with the concentration of 0.5 g / L to 10 g / L, a protective agent with the concentration of 8 g / L to 15 g / L, a surfactant with the concentration of 1 g / L to 5 g / L and inorganic metal salt with the concentration of 0.5 g / L to 5 g / L; the compound oligomeric antibody is a mixture of a mouse anti-human glycosylated hemoglobin antibody polymer and a rabbit anti-mouse IgG antibody polymer in a mass ratio of 1: (0.5-1.5); the kit is good in linear relation, high in accuracy, high in sensitivity, high in precision and high in specificity, provides a more reliable basis for clinical diagnosis, can be applied to a wider range of temperatures and can meet the requirements of clinical examination under different conditions.

The present invention relates to humanized monoclonal antibodies comprising the CDRs of murine antibody BMA031, which bind to the apTCR.CD3 complex and possess improved biological properties.

The invention provides application of colloidal gold immune test paper in detection of human calcitonin, the colloidal gold immune test paper comprises a sample pad, a gold-labeled antibody binding pad, a nitrocellulose membrane and absorbent paper which are sequentially and partially spliced on a PVC (polyvinyl chloride) bottom plate, the gold-labeled antibody binding pad is coated with a human calcitonin mouse specific monoclonal antibody, and the nitrocellulose membrane is coated with the absorbent paper. A detection line and a quality control line which are separated from each other are arranged on the nitrocellulose membrane; the detection line is coated with an anti-human calcitonin specific antibody, and the quality control line is coated with an anti-mouse IgG antibody.

The invention relates to the technical field of immunodetection, and discloses an immunochromatography reagent for detecting an antiproteinase 3 antibody and application, which are used for detecting the antiproteinase 3 antibody in human serum, plasma or whole blood and assisting in diagnosing wegener granuloma. The sample pad is used for bearing a to-be-detected sample; the combination pad is coated with an anti-human immune globulin antibody or an anti-human IgG antibody marked by a marker; the detection line is coated with a recombinant protease 3 antigen, myeloperoxidase antigen fragments related to Wegner granuloma attack are added, and hydrophilic polysaccharides are adopted as spacer arms to be arranged at intervals; the quality control line is coated with a goat anti-mouse IgG antibody; the water absorption pad is used for absorbing liquid in the chromatography process and promoting the liquid to flow unidirectionally; the bottom plate is used for bearing all the components, and all the components are sequentially and mutually assembled on the bottom plate to form a detection test strip; the immunochromatography reagent is extremely simple and convenient to operate, professional laboratory equipment and complex operation skills are not needed, and a detection result can be obtained only by dropwise adding a sample onto the test strip.

The present application provides a monoclonal antibody targeting adenovirus DBP protein, comprising a heavy chain variable region and a light chain variable region, the heavy chain variable region comprising heavy chain complementarity determining regions HCDR1, HCDR2 and HCDR3, and the light chain variable region comprising light chain complementarity determining regions LCDR1, LCDR2 and LCDR3, wherein the amino acid sequences of HCDR1, HCDR2 and HCDR3 are respectively shown as SEQ ID NOs: 1-3, and the amino acid sequences of LCDR1, LCDR2 and LCDR3 are respectively shown as SEQ ID NOs: 4-6. The monoclonal antibody of the present application can be used for efficient detection of multiple types of HAdV DBP.

The invention discloses a detection kit for detecting calprotectin in human excrement, the detection kit is composed of a sample diluent and detection test paper, the detection test paper is composed of a bottom plate, a nitrocellulose membrane, a conjugate pad, a sample pad and absorbent paper, the absorbent paper, the nitrocellulose membrane, the conjugate pad and the sample pad are sequentially overlapped on the bottom plate, a shell is arranged outside the detection test paper, and the shell is connected with the sample pad. An observation window and a sample adding hole are formed in the shell, and the position of the observation window is matched with the position of the nitrocellulose membrane; the position of the sample adding hole is matched with the position of the sample pad; the nitrocellulose membrane is sequentially provided with a T1 detection line coated with an anti-calprotectin monoclonal antibody, a T2 detection line coated with an anti-calprotectin monoclonal antibody and a quality control line C coated with a goat anti-chicken IgY antibody and / or a goat anti-mouse IgG antibody; a calprotectin monoclonal antibody marked by colloidal gold is solidified on the combination pad, and the sample diluent is prepared from sodium chloride, EDTA (Ethylene Diamine Tetraacetic Acid), PEG (Polyethylene Glycol) 20000, Proclin-300 and purified water.

The invention discloses a detection kit for detecting the content of calprotectin in synovial fluid. The detection kit comprises a detection kit shell and a calprotectin immunochromatography test strip, and the calprotectin immunochromatography test strip is arranged in the detection kit shell; the calprotectin immunochromatography test strip comprises a sample pad, a glass fiber combination pad, a coated nitrocellulose membrane and a water absorption pad which are bonded in sequence, an anti-calprotectin antibody marked by colloidal gold is embedded in the glass fiber combination pad and is used for being combined with calprotectin in a sample to be detected to form a compound; the anti-calprotectin monoclonal antibody is coated on a detection line of the nitrocellulose membrane, and the anti-mouse IgG antibody is coated on a quality control line of the nitrocellulose membrane; the colloidal gold labeled anti-calprotectin antibody is embedded in the glass fiber cushion layer, and the image recognition module is used for collecting a tested test paper image and calculating a relative gray value of a detection line and a quality control line, so that a calprotectin concentration interval is judged.