Colloidal gold immunochromatographic test strip for detecting wild-type classical swine fever virus

Abstract

The invention discloses a colloidal gold immunochromatographic test strip for detecting wild-type classical swine fever virus, which consists of water absorbent paper (1), a cellulose nitrate membrane (2), a colloidal gold pad (3), a sample pad (4) and a support (5), wherein the cellulose nitrate membrane contains a detection line which is formed by coating monoclonal antibody HQ06 of anti-classical swine fever virus E2 protein and a quality control line which is formed by coating rabbit anti-mouse IgG antibody; and the colloidal gold pad is combined with colloidal gold-labeled monoclonal antibody 6E10 of the anti-classical swine fever virus E2 protein. The test strip does not react with C-strain of classical swine fever virus, bovine viral diarrhea virus, porcine reproductive and respiratory syndrome virus, transmissible gastroenteritis virus, porcine epidemic diarrhea virus, porcine rotavirus, pseudorabies virus, porcine parvovirus and porcine circovirus type 2, and can accurately and sensitively identify the wild-type classical swine fever virus, thereby having good specificity, sensitivity and repeatability.

Description

technical field [0001] The invention relates to a test strip for identifying swine fever virus, in particular to a colloidal gold immunochromatographic test strip for identifying wild virus of swine fever virus, belonging to the field of diagnosis and identification of swine fever virus. Background technique [0002] Classical swine fever (CSF) is an acute, febrile, contagious disease of pigs caused by classical swine fever virus (CSFV). The World Organization for Animal Health (OIE) included the disease in the list of notifiable animal infectious diseases. my country has used hog choleralapinized vaccine (hog cholerapinized vaccine, HCLV) to prevent and control the large-scale epidemic of swine fever in my country. However, in recent years, swine fever has made a comeback and revived. Many new changes have taken place in its characteristics, and it is still one of the main infectious diseases that endanger the pig industry in my country (Wang Qin. Epidemiology of swine feve...

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Problems solved by technology

[0004] The large-scale epidemic of swine fever in China has been well controlled by the use of rabbit-type attenuated vaccine against swine fever in my country. However, due to the lack of serological markers and supporting differential diagnosis methods, it is difficult to distinguish immune attenuated virus from wild virus through antibody detection. Infection, which is very unfavorable for the purification of swine fever (Qiu Huaji, Tong Guangzhi, Shen Rongxian. Lapinized attenuated vaccine for swine fever - a review of half a century. Chinese Agricultural Sciences, 2005, 38(8): 1675-1685.)

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