110 results about "Viral diarrhea" patented technology

The invention relates to a preparation and application method for treating a swine viral diarrhea biological agent, and belongs to the technical field of biological agents. The method specifically comprises the following steps of: (1) preparing for immunogen preparation, namely, getting swine transmissible gastroenteritis and porcine epizootic diarrhea duplex inactivated vaccine which consists of two viral protein; (2) performing immune procedure; (3) detecting valence of antibody; (4) purifying an egg yolk antibody; and (5) identifying the egg yolk antibody. The preparation and application method has the advantages that the water diluting method is performed and the method is safe, reliable, out of pollution, and low in cost; the treated high-immunity egg can gain the egg yolk antibody with high activity, purity and output and stable performance; the operation method is simple, feasible, and reliable; the industrial production can be carried out conveniently.

The invention discloses a DPO (Dual Priming Oligonucleotide) primer group for porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus and porcine rotavirus detection and application of the DPO primer group. Aiming to a TGEV-N gene, a PEDV-N gene and a PRoV-VP7 gene, a pair of DPO primers respectively, and a multiplex DPO real-time RT-PCR detection method of a porcine epidemicdiarrhea virus, a porcine transmissible gastroenteritis virus and a porcine rotavirus is established. Results show that a detection limit of the method is 5.3*100 copies/microlitre, target gene fragments can be efficiently amplified within an annealing temperature range from 40 DEG C to 65 DEG C, and shown that the method is wide in annealing temperature range; and meanwhile, the DPO primers are strong in specificity, and no non-specific amplification is generated in the PCR process. The multiplex DPO real-time RT-PCR detection method provided by the invention is simple to design, strong in specificity and high in sensitivity and provides a new technological means to rapid and accurate detection of three porcine viral diarrhea infective pathogens of TGEV, PEDV and PRoV.

The invention discloses a quintuple RT-PCR detection method and kit for porcine epidemic diarrhea viruses, porcine transmissible gastroenteritis viruses, porcine rotaviruses, porcine sapoviruses and porcine kobuviruses. The kit comprises ten specificity amplification primers. In the using process of the kit, total RNA of a sample to be detected is subjected to reverse transcription to become cDNA through a 6-basic-group random primer, a detection reaction system in the kit is used for RCR amplification with the cDNA as a template, and whether the sample to be detected is infected with one kind of pathogens or is under mixed infection is determined according to different sizes of amplified PCR segments of different pathogens. On the condition of ensuring specificity and sensitivity, the kit has the advantages of being easy and quick to operate and lowering detection cost and labor intensity, and is suitable for field sample detection.

The invention discloses a formula for traditional Chinese medicines for treating different types of porcine diarrhea. The traditional Chinese medicines in the formula comprise 30-40 parts of rhizoma atractylodis, 30-40 parts of rhizoma atractylodis macrocephalae, 20-30 parts of cortex magnoliae officinalis, 20-30 parts of rhizoma alismatis, 15-20 parts of pericarpium citri reticulatae, 20-30 parts of poria, 20-30 parts of polyporus, 20-40 parts of herba agastaches rugosae, 10-20 parts of herba periliae, 10-20 parts of radix angelicae dahuricae, 15-30 parts of rhizoma pinelliae, 15-30 parts of radix platycodonis, 10-20 parts of pericarpium arecae, 7-10 parts of ramulus cinnamomi, 7-10 parts of rhizoma zingiberis recens and 15-20 parts of radix glycyrrhizae. The formula has the advantages that traditional Chinese medicine compositions are pure traditional Chinese medicine compositions without other antibiotics, and obvious effects of treating bacterial diarrhea, viral diarrhea and particularly, epidemic porcine diarrhea and cold-dampness type diarrhea due to other factors can be realized; the traditional Chinese medicines are free of medicine residues or toxic and side effects or medicine resistance and high in safety on the premise that optional chemical components and the antibiotics are omitted, and recurrence can be prevented; the formula is low in cost and hopefully can be widely popularized in actual animal husbandry and Chinese herbal medicines, and the like.

The antidysenteric pre-mixed preparation for sucking pig is prepared with the materials including wolf's milk 30-60 weight portions, Chinese goldthread 6-10 weight portions, rhubarb 2-5 weight portions, eclipta 10-15 weight portions and phellodendron bark 7-10 weight portions. The antidysenteric pre-mixed preparation may be used in treating viral diarrhea, mixed viral and bacterial diarrhea of various kinds of animal, and has obvious curative effect on pig's epidemic diarrhea.

The invention provides a multiple liquid-phase chip detection method for swine viral diarrhea pathogens and construction of the multiple liquid-phase chip detection method. The method comprises the following steps: according to the gene sequences of the swine viral diarrhea pathogens, screening out suitable TAG sequences, and coupling the suitable TAG sequences to a microballoon; marking TAG complementary sequences to 5'terminals of upstream primers of PCR primers of the pathogens, and marking biotin at the 5'terminals of downstream primers of the PCR primers; and carrying out multiple PCR amplification by utilizing a modification primer, carrying out hybridization on the PCR amplification products and the TAG sequences coupled to the microballoon, detecting the MFI value, and judging thefeminine gender or masculine of the detection result according to the MFI value. With the method, the detection specificity and sensitivity are improved, the qualitative and quantitative detection formultiple pathogens of the swine viral diarrhea is realized, and in addition, the method has the advantages of high throughput, time and labor conservation, strong specificity, high sensitivity and the like, and can satisfy the demand of simultaneously detecting various pathogens.

The invention relates to a preparation method of a recombinant protein, an indirect classical swine fever hemagglutination test kit that is prepared by the protein, and a preparation method of the test kit. The preparation method of the protein comprises following steps: a segment with the gene homeology degree comparing with a bovine viral diarrhea-mucosal disease virus (BVDV) that is lower than 35 percent is selected from a conservative region of a classical swine fever virus (CSFV) gene, thus obtaining a nucleotide sequence S1 that is corresponding to an amino acid A1; a forth amino acid in the nucleotide sequence S1 is modified to an hydrophilic amino acid, thus obtaining an amino acid A2; a corresponding nucleotide sequence S2 of A2 is modified, thus respectively obtaining three new nucleotide sequences; the three new nucleotide sequences are orderly and serially connected by connecting nucleotides, thus obtaining a sequence S9 that is corresponding to an amino acid sequence A3; and a nucleotide sequence S10 is obtained by processing S9 correspondingly, and after artificially synthesizing and digesting S10, connecting S10 with a carrier pGEX-4T-1 and inducing the recombinant carrier into colon bacillus for induction expression, the recombinant protein is obtained by separating and purifying an expression product.

The invention discloses an anti-diarrhea piglet feed. The feed is prepared from the following components in parts by weight: 670 to 680 parts of soybean meal, 65 to 75 parts of corn germ meal, 20 to 28 parts of stone powder, 18 to 24 parts of soybean oil, 75 to 85 parts of expanded soybean, 28 to 32 parts of fishmeal, 27 to 33 parts of 70% lysine, 0.55 to 0.65 of methionine, 3.2 to 3.6 parts of threonine, 18 to 20 parts of calcium hydrogen phosphate, 15 to 17 parts of salt, 3.15 to 3.25 parts of choline chloride, 18 to 22 parts of premix compound, 1.15 to 1.25 parts of feed additive, 0.55 to 0.65 parts of phytase, 4.5 to 5.5 parts of enzyme preparation, 0.35 to 0 .45 parts of antioxidant, and 1.1 to 1.3 parts of sweetener. The anti-diarrhea piglet feed has the advantages that by using theanti-diarrhea piglet feed disclosed by the invention to feed piglets, the bacterial and viral diarrhea of the piglets can be effectively controlled, and at the same time, the occurrence of yellow-white dysentery and red dysentery of the piglets is reduced, and the disease resistance of the piglets is improved; food intake and appetite of the piglets are improved, piglet growth is promoted, energyand nutrient conversion to muscle tissues is promoted, protein synthesis and deposition are promoted, the ketone body lean rate is increased, the piglet body shape is improved, feed conversion is improved, free radicals are scavenged, meat color is improved, various stress responses of the piglets are relieved, and non-specific immunity of the piglets is increased.

The invention discloses a real-time fluorogenic quantitative PCR detection kit for type-I and type-II bovine viral diarrhea-diarrhoea virus and a special primer thereof and a TaqMan probe. The detection kit has advantages of simple operation, strong specificity, high sensitivity and good repeatability for BVDV detection. The detection kit can be used not only for identification of type-I and type-II bovine viral diarrhea-diarrhoea virus and hog cholera virus but also for accurate quantification of type-I and type-II bovine viral diarrhea-diarrhoea virus. The detection kit can be used in fields of detection of raw materials and semi-finished products in vaccine production and detection of dairy products, etc.

The invention discloses a bovine rotavirus, coronavirus, and viral diarrhea virus multi-connected RT-PCR detection method, which is characterized in that according to the detection method, RNA of a to-be-detected sample is taken as an RNA template, and a specificity upstream primer and a specificity downstream primer designed according to hereditary characteristics of the bovine rotavirus, coronavirus, and viral diarrhea virus are taken as a primer to perform RT-PCR proliferation to obtain a proliferation product. Then, electrophoresis detection is performed on the proliferation product with 1.5% agarose gel, and a result is recorded; according to the results of the electrophoresis detection, whether the to-be-detected sample contains the bovine rotavirus, coronavirus, and viral diarrhea virus is determined. The bovine rotavirus, coronavirus, and viral diarrhea virus detection method achieves has the beneficial effect of accurately, rapidly and efficiently distinguishing and diagnosingthe three viral disease with RT-PCR.

A compound yolk antibody preparation for preventing the viral diseases of piglet, including viral diarrhea of piglet is prepared through providing the vaccines of infectious gastroenteritis, epidemic diarrhea and rotavirus, immunizing with them sequentially by three turns, collecting immunized eggs, taking yolk, diluting with aseptic buffer liquid of phosphate, treating by hydroxypropylmethyl cellulose phthalate, filtering, and spraying drying.

The invention discloses a traditional Chinese medicine composition for preventing piglet dysentery and a preparation method thereof. The traditional Chinese medicine composition, by weight, comprises 5-20 parts of bulk pharmaceutical chemicals hawthorn, 5-20 parts of malt, 5-25 parts of pericarpium citri reticulatae, 5-25 parts of medicated leaven, 10-30 parts of radix bupleuri, 10-30 parts of root of Chinese pulsatilla and 10-30 parts of root of common peony. Firstly needed bulk pharmaceutical chemicals are processed to be clean, then drying and smashing are carried out, various bulk pharmaceutical chemical powder are weighted to be sufficiently and evenly mixed, finally split charging is carried out. The traditional Chinese medicine composition can effectively prevent and treat various irritability diarrheas, trophism diarrheas and yellow and white dysentery caused by escherichia coli, has obvious effects on viral diarrheas, can protect and repair intestinal mucosa and recovers digestion capability of piglets.

The present invention is directed to attenuated pestivirus mutants, which have a reduced ability to replicate as exhibited by a small plaque size. The mutations are in the 5′ nontranslated region of the viral genome. These mutant viruses are useful as live vaccines in the control of bovine viral diarrhea, border disease and classical swine fever.

The invention discloses a mycoplasma bovis immune related protein, a detection kit containing the same and application thereof to detection of a mycoplasma bovis antibody. The mycoplasma bovis immunerelated protein is named p28 protein, and the amino acid sequence of the protein is shown in SEQ ID NO.2. A sensitivity test proves that the mycoplasma bovis serum antibody ELISA detection kit (MbH kit) provided by the invention can detect positive serum with the dilution multiple of 1:2560 at the minimum; and a specificity test proves that the kit has the specificity of 97.8% and has no specificreaction with the positive serums of contagious bovine pleuropneumonia (CBPP), foot-and-mouth disease (FMD), bovine tuberculosis (MB), bovine viral diarrhea (BVDV) and infectious bovine rhinotracheitis (IBRV), and has good stability and high accuracy. The invention provides a new technical means for detecting a mycoplasma bovis antibody.

The invention discloses a swine umbilical cord blood swine fever virus wild strain diagnosing primer group, a kit containing the same and application of the swine umbilical cord blood swine fever virus wild strain diagnosing primer group. Swine umbilical cord blood swine fever virus wild strain diagnosing primer are shown in SEQ ID NO: 1-SEQ ID NO: 4. The swine umbilical cord blood swine fever virus wild strain diagnosing primer group can be applied to producing a swine umbilical cord blood swine fever virus wild strain diagnosing kit. The swine umbilical cord blood swine fever virus wild strain diagnosing kit is composed of an outer set of primers, an inner set of primers, negative control, positive control and 2*PCR (polymerase chain reaction) Mixture. The swine umbilical cord blood swine fever virus wild strain diagnosing primer and the kit containing the swine umbilical cord blood swine fever virus wild strain diagnosing primer group can specifically diagnose swine umbilical cord blood swine fever virus wild strains and distinguish swine fever virus vaccine strains from swine fever virus vaccine strains as well as swine fever virus from bovine viral diarrhea-mocosal virus and border disease virus, and meanwhile, are high in sensibility, good in repeatability and reliable in detection results. The detection method through the swine umbilical cord blood swine fever virus wild strain diagnosing primer group is simple in process, short in detecting time and simple in result evaluation.

The invention discloses a pharmaceutical composition, which comprises montmorillonite andzinc oxide at the weight ratio of (0.25-4) to 1. The invention further provides a preparation method of the pharmaceutical composition and an application of the pharmaceutical composition in preparation of medicines for preventing and/or treating piglet diarrhea. A pharmacological experiment proves that the pharmaceutical composition disclosed by the invention is capable of effectively preventing piglet diarrhea and improving the growth performance and the digestion function of piglets. In addition, the pharmaceutical composition disclosed by the invention does not need to use antibiotic in viral diarrhea of primary piglets and diarrhea of early weaning piglets; the piglets are high in survival rate and free of sequela; and the pork is relatively safe and healthy.

The invention provides a compound propolis composition used for treating pig viral diarrhea and a preparation method thereof and relates to the field of animal medicine. The preparation method provided by the invention comprises the following steps of: (1) respectively carrying out superfine grinding on various components (except for interferon) in a prescription, and respectively sieving by virtue of a 700-mesh sieve; (2) weighing 15-35 parts by weight of propolis, 10-20 parts by weight of porcine interferon, 10-35 parts by weight of granatum, 15-40 parts by weight of fructus chebulae, 10-40 parts by weight of scutellaria baicalensis, and uniformly mixing for 5-10 minutes; (3) weighing 10-35 parts by weight of dark plum, 10-35 parts by weight of Chinese knotweed herb, 15-35 parts by weight of round cardamom, 15-35 parts by weight of dried ginger, 15-35 parts by weight of potentilla chinensis, and uniformly mixing for 5-10 minutes; and (4) mixing compositions obtained by the steps (2) and (3), and continuously mixing uniformly for 10-15 minutes, thus the compound propolis composition is obtained. The compound propolis composition provided by the invention has the beneficial effects that the prescription is reasonable, the preparation method is simple, the compound propolis composition has no toxic or side effect, no pollution, no drug residue and no drug resistance, the superfine grinding efficiency and the biological availability are high, the curative effect is obvious, and clinical application shows that the compound propolis composition achieves a special effect when being used for treating pig viral diarrhea.

The invention relates to traditional Chinese medicinal oral liquid for treating bovine viral diarrhea. The traditional Chinese medicinal oral liquid comprises gentiana scabra, amur cork tree barks, folium isatidis, lalang grass rhizome, sanguisorba officinalis, prunella vulgaris, anemarrhena asphodeloides and medicine terminalia fruits. A finished product is prepared by weighing each component according to a proportion, adding water for decoction twice, mixing filtrate, performing concentration, adding ethanol for standing, performing concentration again, adding water for cold storage, finally adding sodium benzoate, and performing filling and sterilization. When the traditional Chinese medicinal oral liquid is used, diseased cattle is fed according to the body weight of the diseased cattle, and after the traditional Chinese medicinal oral liquid is continuously used for a period of time, the condition of the diseased cattle is controlled, body temperature is recovered to be normal, mucus in manure is avoided, and the digestive tract and lymph nodes are recovered to be normal after the diseased cattle is dissected; tests show that harm to viruses to the cattle can be effectively suppressed and medicinal residues and toxic or side effects are avoided.

The present invention provides 17-keto steroid compounds and their derivatives, metabolites and precursors and pharmaceutically acceptable salts thereof for the treatment or prevention of hepatitis C virus and/or hepatitis G virus in patients requiring such treatment, these compounds Collectively referred to as "compounds of the present invention". In addition, the present invention provides methods of treating or preventing togavirus infections including one or more of alphaviruses, flaviviruses (such as yellow fever virus), hepatitis C virus, and hepatitis G virus, Infection with rubella virus or pestiviruses (such as bovine viral diarrhea virus). In addition, the present invention provides combination therapy comprising the administration of one or more compounds described herein and the administration of one or more compounds selected from the group consisting of plasma concentration-enhancing compounds, macrophage stimulating factors, oxidative agents, ribavirin, and alpha interferon compounds and/or oxygen supply. The compounds of the invention may also be used to alleviate or alleviate one or more symptoms associated with togavirus infection.

The invention discloses a pAPN gene site-directed modified pig. The pAPN gene site-directed modified pig is obtained by a plurality of technologies, such as constructing a gene vector of porcine aminopeptidase N (pAPN) by adopting a method in genetic engineering and performing cell transfection. The pAPN gene site-directed modified pig is mainly applied in two aspects: 1, the infection of viral diarrhea and K88 of the pig is thoroughly eliminated from a genetic angle, the disease control cost of pig farming is reduced, the pollution to environment caused by pig farming is reduced, and a method for performing healthy cultivation and reducing the abuse of antibiotics is provided; 2, the gene site-directed modified pig is the trial test before researching the screening and clinic of the pathogenesis and related intervention and treatment of related diseases, such as human cancers.

The invention discloses a deer-derived bovine viral diarrhea inactivated vaccine and a preparation method thereof, relates to the technical field of vaccines, and fills the blank of a vaccine specialfor viral diarrhea/mucosal diseases of sika deer. The deer-derived bovine viral diarrhea inactivated vaccine JL05 is preserved in the CGMCC on January 11, 2018, with a collection number of CGMCC No.15283. The deer-derived BVDVJL05 virus is subjected to passage by using MDBK cells and then is subjected to plaque cloning to purify the virus; detection shows that the content of the deer-derived BVDVJL05 virus is 107.2 TCID50/ml or more, and no germ, mold, mycoplasma or exogenous virus pollution is caused; the deer-derived BVDV JL05 virus meets a virus requirement for vaccine preparation. The virus is inactivated by BEI and then is mixed and emulsified with a 206 adjuvant to prepare the vaccine; safety and potency inspection shows that the vaccine is safe to the sika deer, and the challenge protection rate can be up to 100 percent.

The invention relates to a traditional Chinese medicine composition for treating child virus diarrhea. The traditional Chinese medicine composition comprises nine kinds of medicines including fried coptis chinensis, fried honeysuckle flowers, lotus leaves, coats of hyacinth beans, smoked plum, fried pomegranate bark, raw liquorice, Chinese yam and roasted malt. The traditional Chinese medicine composition gives consideration to both symptoms and root causes, has the effects of nourishing yin to benefitting spleen and elevating clear qi to lower turbid qi, is good in clinical application effect, has no untoward effect, can shorten the courses of diseases, has the rapid antidiarrheic effect, further can prevent the states of the diseases from being further developed and prompt recovery of the spleen and stomach functions; severe acute disease toxicity diarrhea occurrence rate is lowered; the sick child compliance is good; the price is reasonable; and as for numerous toxicity diarrhea sick children, the traditional Chinese medicine composition has the considerable market potential and social benefits.