34 results about "Virus detection" patented technology

The utility model belongs to virus storage equipment technical field, and disclose a kind of virus detection specimen storage device, including storage box, the outside hinged cover of storage box is equipped with, and the inside assembly of storage box is equipped with quick -mount structure, the outside assembly of cover is used for the abutment structure for limiting specimen movement, the quick -mount structure includes the fixed plate installed from top to bottom in the inner wall of storage box, by the design of quick -mount structure, push freezer to make it slide inside baffle, extrude first spring simultaneously, during this period, the slider outside first connecting rod slides along the trajectory of heart-shaped sliding slot, and slides into one place limit slot and is locked, at this time, freezer slides to the outside of storage box, the dry ice or ice bag in freezer can be replaced, after replacement is completed, freezer is pushed again, at this time, slider is separated from first limit slot, and along heart-shaped sliding slot is moved to second limit slot and is locked, freezer is reset, and the quick replacement of dry ice or ice bag etc. in freezer is further achieved.

This invention discloses an SFTSV virus detection kit and method based on LAMP-CRISPR / Cas combined with electrochemistry. By integrating multiple detection technologies, this invention combines the CRISPR / Cas system with LAMP isothermal amplification technology and electrochemical detection technology to achieve more efficient and sensitive nucleic acid detection, greatly shortening the detection time and meeting the needs of rapid clinical diagnosis. The detection exhibits high specificity and sensitivity, enabling accurate detection of samples with extremely low concentrations. The equipment is simplified and portable, facilitating real-time monitoring and automated operation. This invention innovatively combines real-time signal detection with automated operation, enabling real-time monitoring of the amplification process and direct acquisition of results through electrochemical signals, reducing operational difficulty and improving detection efficiency.

The application discloses a parrot beak feather disease virus probe fluorescence quantitative detection primer, a kit and application thereof, and belongs to the technical field of virus detection. The detection primer comprises an upstream primer PBFDV-F with a nucleotide sequence as shown in SEQ ID NO. 3, a downstream primer PBFDV-R with a nucleotide sequence as shown in SEQ ID NO. 4 and a probe PBFDV-P with a nucleotide sequence as shown in SEQ ID NO. 5. The application designs parrot beak feather disease virus specific detection primers and Taq Man probes, constructs a recombinant standard plasmid, optimizes a reaction system and establishes a parrot beak feather disease virus detection method. The sensitivity, specificity and repeatability of the detection method are verified through experiments, and it is found that the detection method has good sensitivity, specificity and repeatability. The application provides reliable technical support for clinical diagnosis and epidemiological investigation of PBFD.

The application provides a primer probe combination for detecting HPV16 and HPV18 by using microdroplet digital PCR and application thereof, and belongs to the technical field of human papilloma virus detection products. The primer probe combination for detecting HPV16 and HPV18 by using microdroplet digital PCR comprises a multi-target primer probe combination for detecting HPV16, and a multi-target primer probe combination for detecting HPV18, wherein the nucleotide sequence of the primer probe combination for detecting HPV16 is shown in SEQ ID NO. 1-27, and the nucleotide sequence of the primer probe combination for detecting HPV18 is shown in SEQ ID NO. 28-54. The primer probe combination has high sensitivity and low detection limit, can accurately detect HPV16 and HPV18 with high specificity and low cost, accurately capture trace ctDNA in early cervical cancer patient samples, significantly improve the early diagnosis rate, and effectively avoid the missed detection risk of single detection.

The application discloses a PE virus detection method, comprising the following steps: determining target resources required by a PE file during runtime; wherein the target resources comprise a CPU, a memory management module and a runtime library; virtually executing the PE file by simulating the target resources, and recording characteristic information generated by the PE file during the virtual execution; and judging whether the PE file is a virus file according to the characteristic information. The application can reduce resource consumption of PE virus detection. The application also discloses a PE virus detection device, a storage medium and an electronic device, which have the above beneficial effects.

This invention provides a PCR primer set and method for detecting bovine rotavirus subtypes, belonging to the field of virus detection technology. The invention designs primers with high specificity and sensitivity for different bovine rotavirus subtypes. The detection method is simple to operate, can greatly shorten the typing and identification time, has high accuracy, and saves costs.

The present application relates to the technical field of network security, and especially relates to an information security situation awareness method based on virus memory function, a medium and equipment, and discloses an information security situation awareness method based on virus memory function, a medium and equipment, which aims at the dynamic diffusion trend of existing network viruses in a network system, establishes a memory knowledge base of viruses in the network system, and adopts different ways for virus detection and killing of terminal equipment that has been infected by viruses, abnormally running terminal equipment and terminal equipment without infection, reduces the utilization rate of system running memory for virus detection and killing, and solves the problem that the overall terminal equipment in the network system presents a dynamic change trend for current network viruses, and if simple virus scanning and killing in a large area is used, a large amount of data needs to be loaded and run, a long time needs to be consumed, system normal running memory is occupied, and system paralysis is caused due to loading and processing of massive data.

This invention discloses a primer set, reagent kit, and their applications for the simultaneous detection of Aleutian virus and Canine Distemper Virus Asia 6 (Asia 6) in red pandas, belonging to the field of endangered wildlife virus detection technology. This invention provides a primer set and reagent kit that specifically identifies Aleutian virus and Canine Distemper Virus Asia 6 in red pandas. Detection can be completed within 20 minutes under constant temperature conditions of 39℃; it does not cross-react with other common pathogens and does not produce false positives due to primer dimer formation. In other words, it features rapid detection, high specificity, and high sensitivity. The implementation process is simple and easy to operate, requiring no specialized testing personnel for on-site testing. The detection is rapid and accurate, and initial applications have shown good results, providing a method for rapid diagnosis, virus tracing, wildlife disease monitoring, and early warning in zoos and wildlife rescue institutions.

A method and system for virus detection in tumor RNA sequencing data are disclosed. The method includes preprocessing the raw tumor RNA sequencing data; inputting the processed tumor RNA sequencing data into sequence-information-based channels and codon-based channels for feature extraction to generate a feature matrix; constructing a sequence information prediction model and a codon information prediction model, and inputting the feature matrices generated from the sequence information-based channels and codon-based channels into the sequence information prediction model and codon information prediction model, respectively, for training and optimization; predicting the virus probability of each sequencing read to obtain a model score; and selecting viral sequencing reads based on the model scores to assemble viral contigs. This invention improves the accuracy and robustness of virus monitoring by introducing a multimodal deep learning method, and can adaptively process sequencing data from different sources and of different lengths, thereby better meeting the needs of medical and research fields for virus identification in tumor sequencing data.

This application relates to the technical field of pseudorabies virus detection, specifically to a primer combination for pseudorabies virus detection, its application, and a reagent kit. Specifically, the amplification primers in this application exhibit good specificity and amplification efficiency when used for pseudorabies virus detection, and demonstrate good linear regression performance, enabling more accurate and precise quantitative detection of pseudorabies virus.

This invention belongs to the field of infectious disease prevention technology, specifically relating to a method for visualizing infectious disease virus fields based on spatiotemporal trajectory data. The method includes: collecting multi-dimensional data related to infectious diseases, including spatiotemporal trajectory correlation data, virus detection data, and patient-related data (based on data from children with diarrhea at Tianjin Children's Hospital from July 2020 to August 2023); cleaning, standardizing, coding, and statistically analyzing the data; constructing a spatiotemporal distribution field, a genotype distribution field, and a risk correlation field based on a spatiotemporal axis; and visually presenting the data using a combination of various charts such as bar charts, line charts, statistical tables, and phylogenetic trees, combined with an interactive interface. This method integrates multi-source data to accurately display the spatiotemporal epidemic characteristics of infectious diseases, the evolutionary patterns of viral genotype distribution, and the correlation between genotypes and patient characteristics. All results are based on measured data, providing scientific support for infectious disease prevention, control, and treatment, and improving the efficiency and accuracy of data application.

The application discloses a method for detecting viruses and virus replication in media based on Small-RNA high-throughput sequencing. The application belongs to the technical field of virus detection, and the method for identifying virus types in a to-be-detected sample by Small-RNA sequencing developed by the application comprises the following steps: 1) extracting total RNA from a virus-infected to-be-detected sample, constructing a cDNA library, and separating small RNA sequence fragments from the cDNA library; 2) downloading virus sequences and classification databases from GenBank, and comparing the small RNA sequence fragments with virus sequence files; 3) comparing a contigs3 file with a virus nucleic acid database, selecting sequences with the minimum e value in the comparison results in the contigs3 file, calculating the coverage of the contigs3 sequences in corresponding virus genomes, distinguishing virus information with a coverage threshold, and deducing the virus source of the small RNA sequence.

This invention discloses primers, probes, a kit, and a method for detecting TobRFV brown wrinkled fruit virus based on MIRA technology, belonging to the field of plant virus detection technology. The primers and probes include an upstream primer ToBRFV-ORF1-3F3, a downstream primer ToBRFV-ORF1-3R6, and a probe ToBRFV-ORF1-P3. The nucleotide sequence of the upstream primer ToBRFV-ORF1-3F3 is shown in SEQ ID NO:15, the nucleotide sequence of the downstream primer ToBRFV-ORF1-3R6 is shown in SEQ ID NO:21, and the nucleotide sequence of the probe ToBRFV-ORF1-P3 is shown in SEQ ID NO:2. The kit of this invention can achieve highly sensitive and specific rapid amplification of ToBRFV nucleic acid under isothermal conditions, and is suitable for rapid on-site detection in non-laboratory environments such as fields and greenhouses.

1. Name of the product in this design: Packaging Box (Human Astrovirus Detection Kit). 2. Purpose of this design: for packaging. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

A simple, high-speed, and highly sensitive on-site virus detection device is provided. It comprises: a receptor-like membrane (13) with multiple receptor-like structures (14) arranged to mimic host cell receptors that specifically bind to the target virus; a virus delivery piping (A) forming a piping system that draws in the air to be tested (31a), compresses the air into a high-speed airflow containing the target virus (33b), concentrates the target virus contained in the air, and sprays the high-speed airflow onto the receptor-like membrane; a signal converter (12) converting a physical signal indicating a change in the physical state of the receptor-like membrane due to specific binding of the receptor-like structure to the target virus into an electrical signal; and a signal processing unit (50) performing integral differential detection based on the output data of the signal converter to detect specific binding of the receptor-like structure to the target virus.

This invention relates to the field of pathogen detection technology, specifically to an intelligent adaptive virus detection and optimization method and system. The method includes: acquiring raw sequence data generated by probe capture sequencing; performing real-time analysis of the sequence data using a deep learning model, outputting analysis results including the virus's presence state and sequence characteristics; dynamically adjusting control parameters of the hybridization capture process based on the analysis results through an adaptive control system; and completing hybridization capture according to the adjusted parameters and outputting a detection report. The system includes a sequencing data acquisition module, an analysis module, a sensor data acquisition module, an adaptive control module, and a detection report generation module. This invention, by combining artificial intelligence analysis with real-time process control, achieves intelligent optimization of the virus detection process, significantly improving the accuracy, sensitivity, and adaptability of detection. It is particularly suitable for detecting highly variable viruses and low-viral-load samples, and has significant clinical application value.

1. Name of the product in this design: Graphical User Interface for Virus Detection and Removal of Electronic Devices. 2. Intended use of this design: electronic equipment. 3. The key design feature of this product is its graphical user interface. 4. The image or photograph that best illustrates the design's key features: the front view. 5. Purpose of the graphical user interface: The graphical user interface of this design is the interactive interface for virus scanning in the vehicle system. The main view is the homepage interface, displaying operation instructions and a "Start Scan" button. Clicking "Start Scan" initiates the scan, and the shield icon displays a scanning animation. A scan progress bar and "Cancel" and "Pause" buttons are displayed to the right of the icon, as shown in Figure 1. In Figure 1, after the scan is complete, if virus files are detected, an exclamation mark appears in the shield icon, and the number of viruses and a button to view virus details are displayed to the right, as shown in Figure 2. In Figure 2, clicking "View Details" takes you to the details list page, where users can choose to scan and remove individual viruses or perform a one-click scan and remove all viruses, as shown in Figure 3. In Figure 3, after clicking "One-Click Scan" to remove all viruses, a checkmark appears in the shield icon, and the "Start Scan" button is displayed to the right of the icon, as shown in Figure 4. In each view, characters represented by the letter 'X' are replaceable, and the number of 'X's does not limit the number of characters that can be replaced. The white area represents the avatar content.

1. The name of the design product: nucleic acid detection box. 2. The use of the design product: the design product belongs to the field of virus detection and is used for the detection of viral nucleic acid. 3. The design points of the design product: the shape. 4. The picture or photo that best indicates the design points: perspective view.

The application discloses a paper-based sensor chip for multiple respiratory virus detection and a preparation method and application thereof. The paper-based sensor chip takes filter paper as a base material and is provided with a sample adding area (100), a working electrode area (200), a reference electrode area (300) and a counter electrode area (400). The working electrode area (200) is provided with a plurality of working electrodes which are arranged in a row matrix in a direction away from the sample adding area (100). The working electrode takes graphene as a conductive channel, and the surface of the working electrode is fixed with a nano-gold-streptavidin composite material and a capture probe covalently combined with biotin. The upper side of the working electrode is the reference electrode area (300), the lower side of the working electrode is the counter electrode area (400), and the working electrode, the reference electrode area (300) and the counter electrode area (400) form a three-electrode system. The paper-based sensor chip can realize efficient and synchronous detection of multiple viruses and has good application value.

A biosafety laboratory is disclosed. This laboratory comprises multiple inflatable membrane structure virus detection laboratories. The inflatable membrane structure of each laboratory forms a high-contamination zone. Transfer units are suitable for being installed at the first and second transfer ports of this high-contamination zone. The inflatable membrane structure includes an inflatable membrane dome structure, and the functional space is defined by the inflatable membrane dome structure after inflation. The inflatable membrane dome structure includes an outer support frame and an inner single-layer membrane, which is adapted to form a positive-pressure air-membrane space between itself and the support frame. The second transfer port of one of the inflatable membrane structure virus detection laboratories is aligned with the first transfer port of an adjacent inflatable membrane structure virus detection laboratory and connected via a transfer unit. The inflatable membrane structure can be inflated to form a roof or tubular structure, facilitating the rapid construction of the inflatable membrane structure virus detection laboratory and enabling it to be put into use as soon as possible.

An air-supported membrane structure virus detection laboratory. The laboratory comprises: an air-supported membrane structure, which can form a clean area, a buffer unit and a high-pollution area after being inflated, the clean area, the buffer unit and the high-pollution area are all negative pressure environments, and the pressure relationship of the clean area, the buffer unit and the high-pollution area is: clean area > buffer unit > high-pollution area. The air-supported membrane structure can form a roof or a tubular body through inflation, which is conducive to realizing rapid construction of the air-supported membrane structure virus detection laboratory and putting the air-supported membrane structure virus detection laboratory into use as soon as possible; in addition, the high-pollution area is a negative pressure environment, which is conducive to ensuring the use safety during detection of viruses.

The application discloses a kind of silkworm egg microparticle virus intelligent detection methods, comprising S1: collecting the continuous multiple frame microscopic image sequence of silkworm egg sample;S2: generate single-frame clear image after deblurring;S3: output virus candidate frame and its initial confidence;S4: the virus candidate frame and its initial confidence are input to a detection credibility verification module of uncertainty perception and explainability enhancement, and generate gradient weighted class activation mapping heat map, according to the prediction uncertainty variance and image quality dynamic adjustment confidence threshold, output final detection result, this kind of silkworm egg microparticle virus intelligent detection method improves the recovery quality of complex motion blurred image, while, reduce the false detection rate and the missed detection rate of small virus detection, second, provide the uncertainty quantification and explainability output of detection result.

The application discloses a microfluidic chip pathogen rapid detection reagent based on nano-antibody two-photon signal amplification and application thereof in rapid diagnosis of influenza A virus, influenza B virus and syncytial virus. The two-photon signal amplification system is pre-integrated in the microfluidic chip by adopting ultrasonic wave spraying modification PMMA base material and combining with piezoelectric ceramic nozzle precise spraying pL level droplets. During detection, UCNPs mark specific antibody I is combined with three virus target antigens, and then is combined with PLNPs mark specific antibody II on the chip to complete the multi-target synchronous quantitative detection by means of the fluorescence signal. The application overcomes the short board of traditional virus detection, can reach pg / ml level high-specificity detection, realizes rapid and accurate triple virus screening by means of microfluidic automatic operation, and provides a new type of efficient and feasible technical scheme for synchronous rapid detection of the three respiratory viruses.

This invention discloses a liquid-phase probe-targeted capture library construction method and kit for enteroviruses, belonging to the field of virus detection technology. This invention combines targeted enrichment with high-throughput sequencing, featuring broad coverage, high flexibility, high sensitivity, and high specificity, enabling multi-dimensional information output including quantitative, qualitative, and in-depth analysis. Through the probe-targeted capture "enrichment-screening" mechanism, this invention reduces probe redundancy design, minimizes background nucleic acid interference from intestinal flora, exhibits excellent adaptability to complex samples, and eliminates the need for complex sample pretreatment, making it suitable for large-scale epidemiological detection.

This invention discloses a semi-quantitative RT-LAMP method and kit for viral nucleic acid detection based on dispensed lyophilized microspheres and a multi-channel microfluidic chip. The method includes: designing two independent LAMP primer sets (P1 and P2) for each of the SARS-CoV-2 Omicron strain N gene, Zika virus NS5 gene, H1N1 influenza virus M1 gene, and H3N2 influenza virus NP gene; preparing two independent types of lyophilized microspheres by independently mixing RT-LAMP premix containing Bst DNA polymerase and other components with LAMP primer mixture and a lyophilization protectant; and using an N-channel microfluidic chip to perform serial dilutions and parallel RT-LAMP amplification of the same sample, determining the concentration of viral nucleic acid in the sample by the positive / negative inflection points of each channel. This invention is simple to operate, requires no equipment such as a real-time PCR instrument, and is suitable for rapid virus detection in various scenarios.