366 results about "Virus detection" patented technology

The invention belongs to the field of virus detection and diagnosis, and relates to an anti-AAV2 monoclonal antibody as well as a preparation method and application thereof. The invention provides an anti-AAV2 rabbit monoclonal antibody, and amino acid sequences of a heavy chain variable region and a light chain variable region of the anti-AAV2 rabbit monoclonal antibody. The anti-AAV2 rabbit monoclonal antibody provided by the invention can be specifically combined with the VLP of AAV2, and can be used for detecting the AAV virus antigen. The anti-AAV2 monoclonal antibody provided by the invention provides an effective detection tool for the detection of AAV2 in the research and development process of a gene therapy vector and the detection of AAV2 in basic scientific research.

The embodiment of the invention discloses a terahertz virus spectrum classification method and system based on transfer learning, and the method comprises the steps: obtaining a source domain terahertz spectrum data set containing various biomolecule hydrogen bond vibrations, and obtaining an initial feature extraction model; extracting a hydrogen bond vibration characteristic mode in a preset frequency band range, and taking a characteristic center of the hydrogen bond vibration characteristic mode as a cross-domain bridging characteristic; obtaining target sample data, wherein the sample data is a small sample data set; converting the similarity between the feature center and a target sample spectrum peak into a dynamic weight of a migration loss function, and performing layer-by-layer fine adjustment on the initial feature extraction model network to obtain a virus classification model; the fine tuning process comprises the following steps: adjusting a weight factor in real time according to the spectrum peak offset of the current batch of samples; inputting the terahertz spectrum data of the to-be-detected virus into the virus classification model to obtain a classification result of the to-be-detected virus. According to the application, the accuracy, robustness and industrial applicability of terahertz virus detection are remarkably improved.

The invention discloses a panda-derived feline panleucopenia virus RAA-CRISPRCas12a detection method, and belongs to the technical field of virus detection, and the panda-derived feline panleucopenia virus RAA-CRISPRCas12a detection method comprises the following steps: S1, primer and probe design; s2, screening an RAA amplification primer; s3, RAA reaction condition optimization; s4, performing RAA sensitivity and specificity verification; s5, preparing crRNA (Complementary Ribonucleic Acid); s6, optimal crRNA screening is carried out; s7, optimizing the RAA-CRISPR / Cas12a (Reverse Amplified Architecture- S8, the specificity and the sensitivity of the RAA-CRISPR / Cas12a are verified; s9, clinical sample detection; according to the invention, by elaborately designing a primer and a probe aiming at the capsid protein VP2 gene of the feline parvovirus (FPV), fully utilizing the advantages of recombinase-mediated amplification (RAA) and a CRISPR / Cas12a system, and comprehensively optimizing reaction conditions and components of the system, the detection method has remarkable advantages, can quickly complete the detection process under the condition of constant temperature of 37 DEG C, is extremely high in sensitivity, and can be used for rapidly detecting the feline parvovirus. The lowest detection limit can reach 1 * 10 < 1 > copies / mu L, the specificity is high, and the coincidence rate with a real-time fluorescent quantitative PCR method is as high as 100%.

The invention discloses an integrated remote teaching assistance and management system for an education scene, belongs to the field of education informatization, and solves the problems that a traditional education system is split in function, tedious in process, high in safety risk and the like. The system integrates three modules: 1) remote call broadcast: supporting home / school / student real-time broadcast notification (including accessory poison check); 2) remote examination and approval: processing and configuring a multi-level examination and approval process, performing state synchronization and preventing tampering of block chain evidence storage; and 3) auxiliary teaching: supporting functions of courseware uploading, homework arrangement, statistics and the like. The data is stored in a relational database (such as MySQL), and the full-life-cycle security of the data is guaranteed through multi-factor authentication, RBAC permission control, high-strength encryption and block chain evidence storage. The beneficial effects are that function integration improves efficiency; security is enhanced through multi-factor authentication and the block chain; an approval specification process can be configured; visual report auxiliary decision making; a high-availability technology (such as WebSocket heartbeat) is guaranteed to be stable, and a whole-process digital and safe teaching management scheme is provided for schools.

The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus, and belongs to the technical field of genetic engineering. The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus. The pseudovirus contains a nucleic acid fragment of a Nipah virus N gene of which the nucleotide sequence is shown as SEQ ID NO.1. The invention further provides a preparation method of the pseudovirus. The pseudovirus of the Nipah virus can be used as a ribonucleic acid standard substance of the pseudovirus of the Nipah virus, and is applied to the aspects of quantity transmission, calibration, instrument testing, evaluation and control of a testing method, quality verification, quality control, production process detection and the like of a laboratory related to Nipah virus detection. The pseudovirus of the Nipah virus, as the ribonucleic acid standard substance of the pseudovirus of the Nipah virus, has the following advantages of accurate fixed value, good uniformity, stable quantity value and traceability, and can promote the improvement of the detection capability and the technical level of a national Nipah detection laboratory.

The invention relates to the technical field of image processing, and discloses an improved StarNet-YOLOv13-based unmanned aerial vehicle field tobacco virus disease lightweight detection method, which comprises the following steps: acquiring a to-be-detected image of a field tobacco plant, and then inputting the to-be-detected image into a trained tobacco virus detection model to obtain a detection result of the tobacco plant; wherein the tobacco virus detection model is obtained by replacing a backbone network with a star network, replacing a C3K2 module in a neck network with a feature fusion module and replacing a detection head with a DetectMBConv detection head in a YOLOv13 model; the feature fusion module is constructed by introducing a partial convolution mechanism into the C3K2 module; thus, through the star network (StarNet), the feature fusion module (DSC3k2PConv module) and the DetectMBConv detection head, the small target scab detection capability and the complex scene robustness can be improved while the parameter and calculation overhead can be reduced, so that the tobacco virus detection model can meet the deployment requirement of the computing power limited equipment side, such as an unmanned aerial vehicle, and the detection precision is ensured at the same time.

The invention relates to the technical field of avian virus detection, and particularly discloses a primer probe combination, a kit and a method for simultaneously detecting chicken infectious anemia virus and chicken circle virus type 3 based on fluorescent RAA and application. The method can be used for simultaneously detecting the chicken infectious anemia virus and the chicken circle virus type 3, and has the advantages of simplicity in operation, short detection time, good specificity, high sensitivity, no need of expensive instruments and equipment, support of on-site rapid detection, suitability for large-scale popularization and application and the like; and effective technical support can be provided for rapid detection and screening of the chicken infectious anemia virus and the chicken circle virus type 3.

The embodiment of the invention provides a virus interception method and device, electronic equipment and a storage medium. The method comprises the steps of starting a target drive program in an operating system in response to a starting instruction of the operating system; acquiring a rule set by the target driver, wherein the rule set comprises a plurality of virus rules; in the process of loading the starting drive program, obtaining first program information of the starting drive program by the target drive program; the target driving program performs virus identification on the starting driving program based on the first program information of the starting driving program and the rule set to obtain a virus identification result; and if the virus identification result indicates that the starting drive program is the virus, intercepting the starting drive program. By the adoption of the method, virus detection can be carried out before the starting drive program is started, interception is carried out when it is detected and confirmed that the starting drive program is the virus program, and therefore the safety of an operating system is improved.

The invention discloses a primer group for identifying six different genotypes of IBV epidemic strains and a high-resolution melting curve detection method, and relates to the technical field of virus detection. The primer group for identifying the six IBV epidemic strains with different genotypes is HRM-F / HRM-R, and the nucleotide sequences of the primer group are shown as SEQ ID NO. 1 and SEQ ID NO. 2. By utilizing the specific primer group and the detection method disclosed by the invention, qualitative and quantitative analysis on single infection or mixed infection of IBV epidemic strain genotypes of LX4 (GI-19), Mass (GI-I), 4 / 91 (GI-13), LDT3-A (GI-28), Taiwan (GI-7) and CK / CH / LSC / 99I (GI-22) can be realized. The detection sensitivity is high, the specificity is good, the accuracy is high, the operation is simple and rapid, the cost is low, and the method is suitable for popularization.

This application relates to the field of virus detection technology, specifically disclosing a real-time fluorescent PCR detection kit and method for H10N7 avian influenza virus. The kit includes sample lysis extract, sample washing solution I, sample washing solution II, and reaction premix for detecting H10N7 subtype avian influenza virus; the detection method is an integrated detection method. This application achieves simultaneous detection of H10 and N7 subtype AIVs by designing specific primers and probes. The kit prepared using these primers and probes not only improves detection efficiency but also significantly enhances sensitivity and specificity. The combined use of MAOPA technology and real-time quantitative PCR technology further improves the accuracy of the detection results, making this kit of this application of significant application value in avian influenza prevention and control.

The invention belongs to the technical field of virus detection, and discloses a digital PCR (polymerase chain reaction) kit for simultaneously detecting African swine fever virus, porcine reproductive and respiratory syndrome virus and swine fever virus, and the kit comprises primers and probes as shown in SEQ ID NO.1-SEQ ID NO.24. After a single system of an African swine fever virus-VP72 gene, an African swine fever virus-CD2V gene, an African swine fever virus-14L gene, a porcine reproductive and respiratory syndrome virus-RdRP gene, a porcine reproductive and respiratory syndrome virus-European strain, a porcine reproductive and respiratory syndrome virus-American strain, a swine fever virus-vaccine strain and a swine fever virus-wild strain is tested, a nine-fold digital PCR system containing an internal standard is established; through tests of indexes such as linearity, specificity, minimum detection limit, repeatability, comparison of a single digital PCR system and a multiple digital PCR system, the currently developed digital PCR multiple detection kit completely meets development requirements and is high in specificity and sensitivity, and the minimum detection limit of the digital PCR multiple detection kit can reach 10 Copies / mu L or below.

The unmanned aerial vehicle underwater virus detection and concentration device comprises an unmanned aerial vehicle body, a lighting device is arranged in the middle of the upper end of the unmanned aerial vehicle body, wings are arranged on the four faces of the upper end of the unmanned aerial vehicle body, a first motor is arranged in the unmanned aerial vehicle body, and a rotating shaft is arranged on a driving shaft of the first motor; a cable fixed to the shell is wound on the rotating shaft, a groove matched with the shell is formed in the bottom of the unmanned aerial vehicle body, the lower end of the shell is connected with an assembling structure, and the lower end of the assembling structure is fixedly connected with a water taking structure; the assembly structure is used for accommodating a water sample and simultaneously implementing a virus concentration process; the water taking structure is used for taking water and sampling the water body. The device has the characteristics of safety and high convenience, and can realize quick fixed-point sampling and virus concentration in a water area.

The invention provides a Marburg virus detection standard substance as well as a preparation system and application thereof, and belongs to the technical field of biological detection. The armored RNA standard substance containing the Marburg virus NP gene segment, provided by the invention, contains the NP gene segment, can be used for nucleic acid detection of the Marburg virus taking the NP gene as a target spot, and is used for quality control of detection methods and reagents such as common PCR (Polymerase Chain Reaction), fluorescent quantitative PCR, digital PCR, LAMP (Loop-Mediated Isothermal Amplification) and the like. The armored RNA standard substance provided by the invention has the advantages of high purity, no plasmid DNA residue and good quality control performance. The armored RNA virus-like particle simulates the structure of a natural virus, and can participate in links such as nucleic acid extraction and amplification as an animal body or human body blood sample containing Marburg virus, so that the whole-course quality control of the whole detection process is realized, and the authenticity and reliability of the detection result are ensured.

The invention provides an RPA-PAND detection kit and detection method for canine parvovirus and canine adenovirus, and belongs to the technical field of canine virus detection.The kit comprises PfAgo protein and a gDNA and MB combination used for detecting canine parvovirus MVC, canine parvovirus CPV and canine adenovirus CAV. The RPA-PAND rapid nucleic acid detection method for detecting canine parvoviruses MVC and CPV and canine adenovirus CAV is successfully established on the basis of PfAgo protein mediated target detection and in combination with the RPA technology, and the method has the advantages of high sensitivity, high specificity, low cost, relatively low position targeting limitation, simple and convenient reaction system, no aerosol pollution, capability of performing multi-channel nucleic acid detection and the like; and a new reagent and a new way are provided for clinical diagnosis and epidemiological monitoring of viruses MVC, CPV and CAV.

The application discloses a virus detection model training method and device and a storage medium, relates to the computer field, and is used for realizing accurate detection of a Mirai virus. The method comprises the following steps: acquiring a plurality of executable files, determining a first function call graph in the plurality of function call graphs, and determining at least one second function with a function symbol similarity to the first function greater than a first threshold value; determining a third function with the greatest function symbol similarity to the first function in the at least one second function; adding function attribute information of the third function to the first function call graph as attribute information of the first function, and determining a second function call graph; comparing function information in the second function call graph with function information in a virus database, and determining whether the executable file corresponding to the first function call graph is a virus file; and if yes, training a virus detection model based on the second function call graph. Embodiments of the application are applied to the process of virus detection.

The invention discloses a TOCV fluorescence detection probe primer group, a detection method, a kit and application, and belongs to the technical field of virus detection. The invention solves the problems that the detection of ToCV in the prior art depends on RT-PCR technology, needs high-end large-scale equipment, consumes long time and is difficult to meet the real-time field monitoring requirement. The invention provides a TOCV fluorescence detection probe primer group which comprises a probe and an upstream primer, wherein the sequence of the upstream primer is shown as SEQ ID No.1; the sequence of the downstream primer is shown as SEQ ID No.2. The invention provides a detection method, a kit and application based on the primer. The tomato chlorosis virus is detected through an MIRA fluorescent rapid detection technology, a specific probe primer group is provided, transcription is not needed, detection including amplification and fluorescent detection can be completed within 25 minutes at the soonest, complex instruments are not needed, 37-43 DEG C constant-temperature detection is achieved, field portable equipment is compatible, direct experiments can be carried out in the field, and the kit is high in sensitivity and high in sensitivity. Screening of virus-carrying plants in a seedling raising period or before dispatching is facilitated, and virus diffusion is blocked.

The application discloses a Trojan horse virus detection device and method, and belongs to the technical field of information security. The application first finds that the Trojan horse virus may steal information by controlling a computer loudspeaker to emit ultrasonic signals, and proposes the Trojan horse virus detection device and method in light of the finding. The ultrasonic signals emitted by the computer loudspeaker are collected, and then filtered and amplified. The processed ultrasonic signals are decoded to obtain corresponding spectrum information and further analyzed. If the signal peak values all exceed a preset threshold value within a preset sampling period, it is determined that the device to be detected is invaded by the Trojan horse virus. In this way, the application can effectively detect the Trojan horse virus which steals the information stored in the device to be detected by controlling the loudspeaker of the device to be detected to emit ultrasonic signals, and is beneficial to maintaining the information security of the device.

The invention relates to the technical field of virus detection, and discloses a method and a kit for detecting Chinese sacbrood virus. The method comprises: firstly, carrying out isothermal amplification on a CSBV specific target sequence in a sample nucleic acid by using an RPA technology, the RPA technology adopting a specific primer pair, the sequences of which are SEQ ID NO: 4 and SEQ ID NO: 5; then, an amplification product is mixed with a CRISPR / Cas12b detection system, and the system comprises Cas12b protein, specific sgRNA with the sequence shown as SEQ ID NO: 2 and a single-chain DNA reporter probe marked by fluorescence; when a CSBV target exists, the activated Cas12b protein trans-cleavage reporter probe generates a detectable fluorescence signal, so that rapid and high-sensitivity detection of the CSBV is realized. The invention further provides a detection kit suitable for the method. The detection kit comprises the primer pair, sgRNA and Cas12b protein. When the CSBV is detected based on the method and the kit, complex instruments are not needed, the operation is simple and convenient, the detection time is shorter than 1 hour, the sensitivity and the specificity are high, and the method and the kit are suitable for onsite rapid detection in a bee field.

The invention belongs to the technical field of virus detection, and particularly relates to a primer probe composition for detecting Japanese encephalitis virus and application, the primer probe composition comprises a probe, an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is shown as SEQ ID No.3, and the nucleotide sequence of the downstream primer is shown as SEQ ID No.6. According to the primer probe composition, the invention provides a non-diagnostic method for detecting the Japanese encephalitis virus, and the method has the advantages of rapidness, real time, strong specificity, high sensitivity, simplicity and convenience in operation and suitability for various fluorescence detection equipment, and can accurately detect the Japanese encephalitis virus from a sample to be detected.

The invention relates to the technical field of antibodies, and discloses an antibody for specifically recognizing porcine pseudorabies virus gB (507-734 aa) or an antigen binding fragment thereof and application. The antibody or the antigen binding fragment thereof comprises a heavy chain and a light chain; the antibody or the antigen binding fragment thereof can be used for detecting the existence or the level of the porcine pseudorabies virus gB protein in a sample, and has key significance on optimization of a porcine pseudorabies virus detection method, improvement of diagnosis accuracy and realization of effective prevention and control of epidemic situations.

Embodiments of the present invention disclose a method and apparatus for detecting digital signature certificates. The method comprises: receiving a digital signature certificate sent by a client for loading a target file; searching a preset digital signature certificate library for a reputation score corresponding to the digital signature certificate; and sending the reputation score to the client, so that the client determines whether to perform virus detection on the target file based on the reputation score.

The application discloses a primer probe set for simultaneously detecting multiple frog viruses, a kit and application thereof, and belongs to the technical field of animal virus detection.The primer probe set for detecting frog viruses comprises a primer group with the sequence shown in SEQ ID NO.1-2 and a probe with the sequence shown in SEQ ID NO.3.According to the D5family NTPase gene sequence of the Ranavirus member, the primer and the probe are designed, and through optimization of reaction conditions, a method for detecting frog viruses based on qPCR is finally established.The method has the characteristics of high specificity, good repeatability, high sensitivity, simple operation and reliability, and has good market application prospect and popularization and application value.

The application discloses an EHNV virus detection method and system based on dynamic fluorescence intensity monitoring. The method divides the water area to be detected into small areas, obtains water impurity information, carries out fluorescence detection under different impurity characteristics, analyzes fluorescence data to evaluate the influence of impurities on detection accuracy. According to the accuracy influence data, the best sampling area is determined, and the EHNV virus sample is collected, the fluorescence intensity change of the sample is monitored, and finally the EHNV virus information in the water area is obtained. This method can improve the detection sensitivity and accuracy under the interference of impurities, and is suitable for the fields of aquaculture and ecological environment monitoring. The application also provides a corresponding detection system, which comprises a fluorescence detection device, a data analysis module and a sample processing unit, has automatic operation and efficient data processing capacity, and is helpful for preventing and controlling the spread of EHNV virus.

The present application relates to the field of virus detection technology, specifically disclosing a dual microfluidic fluorescent PCR detection kit for the H3N2 subtype of avian influenza virus. The dual microfluidic fluorescent PCR detection kit comprises a sample lysis extract for detecting the H3N2 subtype of avian influenza virus, a sample wash solution I, a sample wash solution II, and a reaction premix. The reaction premix contains three sets of specific sequence combinations: an H3-specific sequence combination, an N2-specific sequence combination, and an internal standard gene-specific sequence combination. By designing specific primers and probes, and performing optimization and screening, the present application enables simultaneous identification of H3 and N2 subtype AIVs in a single reaction. Furthermore, the amplification reactions of the two target genes are free of interference, resulting in high specificity, high sensitivity, and good reproducibility. This kit enables accurate diagnosis of viral infection and has important application value in the prevention and control of avian influenza epidemics.

The invention belongs to the technical field of biological detection, and relates to a Chikungunya virus detection kit and a detection method. The kit is based on the principle of PCR detection and comprises a primer pair, the sequence of a forward primer of the primer pair is shown as SEQ ID NO: 1, and the sequence of a reverse primer of the primer pair is shown as SEQ ID NO: 2. The chikungunya virus detection kit and the detection method can realize rapid, sensitive and specific detection of chikungunya virus based on the principle of PCR detection, and are especially suitable for various application scenes such as clinical samples, human or animal samples, environmental samples, scientific research samples and the like.

The utility model belongs to virus storage equipment technical field, and disclose a kind of virus detection specimen storage device, including storage box, the outside hinged cover of storage box is equipped with, and the inside assembly of storage box is equipped with quick -mount structure, the outside assembly of cover is used for the abutment structure for limiting specimen movement, the quick -mount structure includes the fixed plate installed from top to bottom in the inner wall of storage box, by the design of quick -mount structure, push freezer to make it slide inside baffle, extrude first spring simultaneously, during this period, the slider outside first connecting rod slides along the trajectory of heart-shaped sliding slot, and slides into one place limit slot and is locked, at this time, freezer slides to the outside of storage box, the dry ice or ice bag in freezer can be replaced, after replacement is completed, freezer is pushed again, at this time, slider is separated from first limit slot, and along heart-shaped sliding slot is moved to second limit slot and is locked, freezer is reset, and the quick replacement of dry ice or ice bag etc. in freezer is further achieved.

The invention discloses a network virus detection method and system, a storage medium and electronic equipment, and belongs to the field of digital network security. The data network virus detection method comprises the following steps: deploying a plurality of file restoration nodes in each region of data network service in a distributed manner; and the file detection node is used for detecting the to-be-detected file. The detection processing comprises the following steps: dividing the to-be-detected file into a first-time detected file and a repeated detected file by utilizing a file buffer pool; carrying out full-quantity file detection on the first-time detected files, and updating the file buffer pool; detecting the repeated files to be detected based on a comparison rule version mode, and updating the file buffer pool; and outputting a detection result of the to-be-detected file. And the file buffer pool is configured with an automatic cleaning mechanism based on time and space. When the file detection rule is updated, the buffer pool cannot be emptied, and only newly added or modified rules need to be detected for the historically scanned file, so that the data processing amount is reduced, and the performance overhead of file detection is saved.

The utility model relates to the technical field of virus detection, in particular to an anti-pollution folding type portable virus detection box. The utility model provides an anti-pollution folding type portable virus detection box which can automatically limit the upper part of a virus storage box when a box cover is closed, and avoids virus sample leakage caused by accidental opening of the cover of the virus storage box. An anti-pollution folding portable virus detection box comprises a box body, a box cover, hinges and the like, and the upper rear portion of the box body is connected with the box cover through the left hinge and the right hinge. The box cover is rotationally closed, the reset spring gradually restores, the sliding frame is driven to move forwards, the pressing cover moves forwards, the pressing cover makes contact with the upper side of the virus storage box, the virus storage box is limited, and the effect that the upper portion of the virus storage box can be automatically limited when the box cover is closed is achieved; and virus sample leakage caused by accidental opening of the cover of the virus storage box is avoided.

The invention belongs to the technical field of virus detection, and particularly relates to a primer composition for detecting an avian influenza NA gene, a detection method and a detection device. The avian influenza NA genes comprise NA genes of an influenza A virus H5N1, an influenza A virus H5N6, an influenza A virus H7N7, an influenza A virus H7N9 and an influenza A virus H9N2, and the primer composition comprises nucleotide sequences as shown in SEQ ID No.1-25. The primer composition for detecting the NA gene causing the highly pathogenic human and livestock co-infected avian influenza, provided by the invention, can be used for detecting whether a sample contains the NA gene causing the highly pathogenic human and livestock co-infected avian influenza, namely NA genes of influenza A virus subtypes H5N1, H5N6, H7N7, H7N9 and H9N2, and a result can be conveniently, quickly and accurately detected; in a practical application process, a detection result can be obtained within 40 minutes.