718 results about "Virus detection" patented technology

The invention belongs to the technical field of virus detection, and particularly relates to a freeze-dried PCR reagent and kit for respiratory virus nucleic acid detection. Positive control; performing negative control; the reaction freeze-dried powder comprises an optimized buffer system, Taq DNA polymerase, reverse transcriptase, dNTP / dUTP Mix, a freeze-drying protective agent, a specific primer and a probe; the specific primer and the probe comprise an oligonucleotide upstream primer for specifically amplifying an M gene conserved region on an influenza A virus genome; by preparing reaction freeze-dried powder, an oligonucleotide primer, a probe, Taq DNA polymerase, reverse transcriptase, dNTP, an optimized buffer system, a freeze-drying protective additive and the like are prepared into components which stably coexist at normal temperature, the detection performance is consistent with that of a liquid reagent, normal-temperature transportation and storage can be realized, the transportation and storage cost of the fluorescent PCR reagent is greatly reduced, the shelf life is prolonged, and the fluorescent PCR reagent is suitable for industrial production. And operation steps are reduced.

The invention provides a primer probe combination and a kit for detecting porcine diarrhea related viruses, and belongs to the technical field of virus detection. The combination comprises specific primers and fluorescent probes aiming at a porcine epidemic diarrhea virus (PEDV), a porcine group A rotavirus (PoRV-A), a porcine transmissible gastroenteritis virus (TGEV) and a porcine delta coronavirus (PDCoV). The primer probe combination disclosed by the invention can be used for simultaneously detecting and identifying the four viruses mainly causing porcine diarrhea in one PCR reaction, and has the characteristics of high specificity, high sensitivity, simplicity and convenience in operation, rapidness and high efficiency. Therefore, according to the invention, rapid and accurate detection of porcine diarrhea related viruses can be realized, and disease prevention and control and epidemiological investigation of pig farms can be effectively supported.

The invention discloses a visual detection method of New Delhi tomato leaf curl virus, and belongs to a plant virus detection technology in the field of biotechnology. The method comprises the following steps: adding genome DNA extracted from a plant sample to be detected into an RAA reaction system containing an RAA primer, and carrying out isothermal amplification; then adding an isothermal amplification product into a CRISPR / Cas12a detection system containing a specific crRNA fluorescent probe and an ssDNA fluorescent probe, and carrying out a reaction; and finally, carrying out fluorescence signal detection on the reaction product, if the fluorescence signal is positive, indicating that the New Delhi tomato leaf curl virus is detected in the plant sample, otherwise, indicating that the New Delhi tomato leaf curl virus is not detected. The method has the advantages of being good in specificity, short in detection time, high in sensitivity, free of dependence on expensive instruments and the like, and the result can be accurately detected through an instrument or directly and preliminarily interpreted in a field with limited conditions.

The invention discloses a network security-oriented adaptive network defense system and method, and relates to the technical field of network attack and defense, and the method comprises the steps: evaluating a basic risk coefficient of user equipment; taking the typical range of the running parameters of the programs in the historical running log as an ideal threshold value of the corresponding program, and judging whether the running parameters of each program exceed the ideal threshold value of the corresponding program or not; according to the to-be-confirmed abnormal program operation data, constructing a network security adaptive situation awareness model, analyzing an abnormal operation index of a real-time program of the equipment, correcting the abnormal index of the real-time operation program based on a basic risk coefficient of the user equipment, and obtaining an abnormal operation index of a corrected program; classifying the abnormal operation indexes of the corrected abnormal programs according to danger levels, and generating corresponding solutions according to classification results; the method has the advantage that the detection accuracy of the non-attack target worm mining virus is improved.

The invention discloses a method for detecting dengue virus based on RPA / RAA / ERA / MIRA-CRISPR / Cas12a, which comprises the following steps: extracting nucleic acid to obtain a sample to be detected, putting the extracted sample in a sterile and disinfected sample container, contacting the sample to be detected with a composition, a product combination, a reagent, a kit, a system or a system, determining the signal change after the sample to be detected is contacted with different substances, and determining the detection result of the dengue virus based on RPA / RAA / ERA / MIRA-CRISPR / Cas12a. In the same container, a to-be-detected sample is in contact with a composition, a product combination, a reagent, a kit, a system or a part used for RPA / RAA / ERA / MIRA nucleic acid amplification in the system, and then is in contact with a part used for CRISPR nucleic acid detection, the RPA / RAA / ERA / MIRA and CRISPR / Cas12a detection methods are combined, a rapid, simple, sensitive and specific dengue virus detection method is realized, the detection sensitivity is high, and the detection time is short. The reaction conditions of virus detection are further optimized.

The invention belongs to the field of virus detection and diagnosis, and relates to an anti-AAV2 monoclonal antibody as well as a preparation method and application thereof. The invention provides an anti-AAV2 rabbit monoclonal antibody, and amino acid sequences of a heavy chain variable region and a light chain variable region of the anti-AAV2 rabbit monoclonal antibody. The anti-AAV2 rabbit monoclonal antibody provided by the invention can be specifically combined with the VLP of AAV2, and can be used for detecting the AAV virus antigen. The anti-AAV2 monoclonal antibody provided by the invention provides an effective detection tool for the detection of AAV2 in the research and development process of a gene therapy vector and the detection of AAV2 in basic scientific research.

The invention belongs to the technical field of plant virus detection, and provides a multiplex RT-PCR detection method for areca-nut related viruses. The primer group for multiple RT-PCR detection of areca-related viruses (areca yellow virus APV1, areca ringspot virus ANRSV and areca shuttle spot virus ANSSV) comprises a first primer group for detecting the APV1, a second primer group for detecting the ANRSV and a third primer group for detecting the ANSSV. The invention also provides a detection kit, which comprises a PCR reaction solution, and the PCR reaction solution comprises the primer group. The invention also provides application of the kit in simultaneous detection of areca-related viruses APV1, ANRSV and ANSSV. The primer group and the kit disclosed by the invention can specifically detect three areca-nut related viruses in a PCR system, can accurately identify any combination infection of the three viruses, and have the characteristics of high efficiency, economy, simplicity and convenience.

A detection system for detecting a virus or the like. The detection system includes an autonomous collection device that is capable of moving on a floor surface and for collecting an object on the floor surface, and a station device for detecting an analyte from the object collected from the floor surface by the autonomous collection device. The autonomous collection device includes a moving part for moving on the floor surface, a primary electric blower for sucking the object on the floor surface, and a dust container for storing the sucked object. The station device includes a transfer pipe fluidically connected to the dust container of the autonomous collection device when the autonomous collection device is positioned in a home position, and a virus detection part for detecting the analyte from the object transferred from the dust container through the transfer pipe.

The invention discloses a method and a system for detecting and evaluating frog viruses in culture water based on tangential flow enrichment, and aims to improve the accuracy and efficiency of virus monitoring in frog culture water. The method comprises the following steps: dividing a culture pond into a plurality of sub-regions, obtaining hydrodynamic parameters of each region, and determining spatial distribution of water flow exchange efficiency; analyzing the growth suitability of the frog virus according to the distribution, and determining the detection position of the virus; detecting viruses in a preset time period by utilizing a tangential flow enrichment technology, and evaluating the change of the virus content; and predicting a virus diffusion trend according to the change data, and providing a corresponding water environment regulation scheme. According to the method, the detection sensitivity and diffusion prediction accuracy of the frog virus can be effectively improved.

The invention provides a ransomware classification method and system based on a large model, and the method comprises the steps: carrying out the feature learning and extraction of preprocessed data through a deep learning large model, and obtaining advanced feature representation, and the deep learning large model is a large model which is subjected to the unsupervised or semi-supervised pre-training, and then is subjected to the fine adjustment based on the feature corpus fine adjustment of SFT supervision; performing preliminary classification and refined classification on the ransomware virus according to the advanced feature representation; and dynamically adjusting model parameters according to the novel ransomware sample. According to the method, by introducing a large model technology, limitation and defects in an existing ransomware detection and classification method are overcome, efficient detection and accurate classification of diversified ransomware viruses are achieved, the generalization ability of the model is enhanced, resource consumption is reduced, and usability and applicability of the system are improved; and the method is suitable for a large-scale data environment and quickly evolved ransomware ecology. According to the method, the overall level of network security defense is remarkably improved, and a more reliable and efficient ransomware virus protection scheme is provided for users.

The embodiment of the invention discloses a terahertz virus spectrum classification method and system based on transfer learning, and the method comprises the steps: obtaining a source domain terahertz spectrum data set containing various biomolecule hydrogen bond vibrations, and obtaining an initial feature extraction model; extracting a hydrogen bond vibration characteristic mode in a preset frequency band range, and taking a characteristic center of the hydrogen bond vibration characteristic mode as a cross-domain bridging characteristic; obtaining target sample data, wherein the sample data is a small sample data set; converting the similarity between the feature center and a target sample spectrum peak into a dynamic weight of a migration loss function, and performing layer-by-layer fine adjustment on the initial feature extraction model network to obtain a virus classification model; the fine tuning process comprises the following steps: adjusting a weight factor in real time according to the spectrum peak offset of the current batch of samples; inputting the terahertz spectrum data of the to-be-detected virus into the virus classification model to obtain a classification result of the to-be-detected virus. According to the application, the accuracy, robustness and industrial applicability of terahertz virus detection are remarkably improved.

The invention discloses a panda-derived feline panleucopenia virus RAA-CRISPRCas12a detection method, and belongs to the technical field of virus detection, and the panda-derived feline panleucopenia virus RAA-CRISPRCas12a detection method comprises the following steps: S1, primer and probe design; s2, screening an RAA amplification primer; s3, RAA reaction condition optimization; s4, performing RAA sensitivity and specificity verification; s5, preparing crRNA (Complementary Ribonucleic Acid); s6, optimal crRNA screening is carried out; s7, optimizing the RAA-CRISPR / Cas12a (Reverse Amplified Architecture- S8, the specificity and the sensitivity of the RAA-CRISPR / Cas12a are verified; s9, clinical sample detection; according to the invention, by elaborately designing a primer and a probe aiming at the capsid protein VP2 gene of the feline parvovirus (FPV), fully utilizing the advantages of recombinase-mediated amplification (RAA) and a CRISPR / Cas12a system, and comprehensively optimizing reaction conditions and components of the system, the detection method has remarkable advantages, can quickly complete the detection process under the condition of constant temperature of 37 DEG C, is extremely high in sensitivity, and can be used for rapidly detecting the feline parvovirus. The lowest detection limit can reach 1 * 10 < 1 > copies / mu L, the specificity is high, and the coincidence rate with a real-time fluorescent quantitative PCR method is as high as 100%.

The invention provides a foot and mouth disease virus detection primer probe and a microdroplet digital PCR absolute quantitative detection kit thereof, and belongs to the technical field of biological detection. The foot and mouth disease virus detection primer probe comprises a forward primer with a nucleotide sequence as shown in SEQ ID NO: 1, a reverse primer with a nucleotide sequence as shown in SEQ ID NO: 2 and a probe with a nucleotide sequence as shown in SEQ ID NO: 3. The invention also provides a foot-and-mouth disease virus microdroplet digital PCR absolute quantitative detection kit. The foot-and-mouth disease virus microdroplet digital PCR absolute quantitative detection kit comprises the foot-and-mouth disease virus detection primer probe, a 5 * one-step probe RT-qPCR main mixed solution, RT-Polymerase Mix, a reference dye, sealing oil and a digital PCR chip. The kit provided by the invention has the characteristics of high sensitivity, strong specificity, high accuracy and high precision, and can realize accurate and quantitative chip digital PCR detection.

The invention discloses an integrated remote teaching assistance and management system for an education scene, belongs to the field of education informatization, and solves the problems that a traditional education system is split in function, tedious in process, high in safety risk and the like. The system integrates three modules: 1) remote call broadcast: supporting home / school / student real-time broadcast notification (including accessory poison check); 2) remote examination and approval: processing and configuring a multi-level examination and approval process, performing state synchronization and preventing tampering of block chain evidence storage; and 3) auxiliary teaching: supporting functions of courseware uploading, homework arrangement, statistics and the like. The data is stored in a relational database (such as MySQL), and the full-life-cycle security of the data is guaranteed through multi-factor authentication, RBAC permission control, high-strength encryption and block chain evidence storage. The beneficial effects are that function integration improves efficiency; security is enhanced through multi-factor authentication and the block chain; an approval specification process can be configured; visual report auxiliary decision making; a high-availability technology (such as WebSocket heartbeat) is guaranteed to be stable, and a whole-process digital and safe teaching management scheme is provided for schools.

The invention discloses an intelligence management method and system based on network security, and relates to the technical field of network security, and the method comprises the steps: setting a firewall between a client network and a server network; performing encryption processing on the key data by using a key encryption algorithm; carrying out virus detection and intrusion detection, and applying an intrusion detection technology and vulnerability scanning in a server network; user identity verification is carried out, a user login process is designed, and login operation of the user is controlled and managed; tracking and positioning the source of the security problem; corresponding server network data processing authority is given to the user; double servers and a disk array are adopted for data storage, and the data are automatically backed up every preset backup cycle. The information collection module and the information management module are arranged to effectively block illegal invasion and virus spreading programs, so that the safety of user information and information is protected, and the confidentiality of the information in the transmission and storage process is ensured.

The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus, and belongs to the technical field of genetic engineering. The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus. The pseudovirus contains a nucleic acid fragment of a Nipah virus N gene of which the nucleotide sequence is shown as SEQ ID NO.1. The invention further provides a preparation method of the pseudovirus. The pseudovirus of the Nipah virus can be used as a ribonucleic acid standard substance of the pseudovirus of the Nipah virus, and is applied to the aspects of quantity transmission, calibration, instrument testing, evaluation and control of a testing method, quality verification, quality control, production process detection and the like of a laboratory related to Nipah virus detection. The pseudovirus of the Nipah virus, as the ribonucleic acid standard substance of the pseudovirus of the Nipah virus, has the following advantages of accurate fixed value, good uniformity, stable quantity value and traceability, and can promote the improvement of the detection capability and the technical level of a national Nipah detection laboratory.

The invention relates to the technical field of image processing, and discloses an improved StarNet-YOLOv13-based unmanned aerial vehicle field tobacco virus disease lightweight detection method, which comprises the following steps: acquiring a to-be-detected image of a field tobacco plant, and then inputting the to-be-detected image into a trained tobacco virus detection model to obtain a detection result of the tobacco plant; wherein the tobacco virus detection model is obtained by replacing a backbone network with a star network, replacing a C3K2 module in a neck network with a feature fusion module and replacing a detection head with a DetectMBConv detection head in a YOLOv13 model; the feature fusion module is constructed by introducing a partial convolution mechanism into the C3K2 module; thus, through the star network (StarNet), the feature fusion module (DSC3k2PConv module) and the DetectMBConv detection head, the small target scab detection capability and the complex scene robustness can be improved while the parameter and calculation overhead can be reduced, so that the tobacco virus detection model can meet the deployment requirement of the computing power limited equipment side, such as an unmanned aerial vehicle, and the detection precision is ensured at the same time.

The invention discloses a hog cholera virus detection system based on cloud analysis, which comprises an image acquisition unit, a data processing cloud, a disease monitoring unit and an early warning management unit, and is characterized in that a live pig limb image is acquired through the image acquisition unit, and an image analysis model is constructed through the data processing cloud to analyze the live pig limb image; the hog cholera risk suspected degree is comprehensively evaluated from two perspectives of vascular risk and tissue edema, so that auxiliary diagnosis is carried out on hog cholera virus detection; the disease monitoring unit is used for monitoring the hog cholera virus for a long time, the deterioration or recovery condition of the disease condition of the sick pig is analyzed, a sick pig health management scheme is generated, and the targeted treatment effect of the sick pig is improved; and the early warning management unit globally analyzes the virus risk and generates a live pig breeding management scheme, so that corresponding overall management and control are performed for the hog cholera virus infection degree of the live pig breeding base, the early warning management efficiency of the live pig breeding base is improved, and the live pig breeding loss and the management cost are reduced.

The invention relates to the technical field of sweet potato virus detection, and provides a sweet potato virus G detection method. The invention relates to a sweet potato virus G specific primer pair. The nucleotide sequence of a forward primer SPVG-F of the specific primer pair is as shown in SEQ ID NO: 1; and the nucleotide sequence of the reverse primer SPVG-R is as shown in SEQ ID NO: 2. According to the technical scheme, the problems of low detection sensitivity and low detection efficiency when a sweet potato G virus amplification primer and a sweet potato G virus same family virus compositely infect a sample in the prior art are solved.

The invention relates to the technical field of virus detection, and particularly discloses a kit for detecting African swine fever virus by using a fluorescent probe method LAMP (loop-mediated isothermal amplification), the kit for detecting African swine fever virus by using the fluorescent probe method LAMP comprises an LAMP reaction solution for detecting African swine fever virus, a specific fluorescent probe and African swine fever virus genome DNA (deoxyribonucleic acid); the LAMP reaction liquid comprises a specific primer combination consisting of a pair of specific outer primers, a pair of specific inner primers and a loop primer; the detection method of the kit comprises the following steps: nucleic acid extraction, fluorescent probe LAMP reaction system preparation, sample amplification and result determination. The kit for detecting the African swine fever virus by using the fluorescent probe method LAMP is simple and convenient to operate, has the advantages of strong specificity, high sensitivity, short time consumption and the like, can efficiently detect the African swine fever virus, and has important application value in prevention and control of African swine fever epidemic situation.

The invention relates to the technical field of network security, in particular to a method, a system and equipment for processing virus characteristic data. The method comprises the following steps: configuring a virtual sandbox virtualization layer, initializing a running environment, setting monitoring parameters, verifying environment isolation and a running state, and generating virtual sandbox running data; acquiring virus sample data, and loading the virus sample data to the virtual sandbox to obtain sample initial state data; according to the sample initial state data and the virtual sandbox operation state data, virtual sandbox operation parameters are configured, a monitoring probe is deployed, and virtual sandbox probe deployment data are obtained; a behavior acquisition channel is established based on virtual sandbox probe deployment data, and a behavior data acquisition model is constructed. According to the method, the virus sample behavior analysis is carried out by constructing the virtual sandbox environment, the virus feature recognition process is optimized by combining feature extraction, dimension reduction and clustering analysis, and the virus detection efficiency, accuracy and real-time response capability are improved.

The invention discloses a kit for simultaneously detecting bluetongue virus, foot and mouth disease virus, schmallenberg virus, peste des petits ruminants virus and sheep pox virus, and belongs to the technical field of virus detection. The kit comprises primers as shown in SEQ ID NO. 1 to SEQ ID NO. 18 and a probe as shown in SEQ ID NO. 18. After a single system of bluetongue virus, foot-and-mouth disease virus, schmallenberg virus, peste des petits ruminants virus and capripox virus is tested, a sextuple system containing an interior label is established, and through testing of indexes such as linearity, minimum detection limit, specificity, precision and repeatability, NTC repetition, comparison of multiple fluorescent RT-PCR and single fluorescent RT-PCR, the kit can be used for detecting the virus resistance of the peste des petits ruminants virus and the capripox virus. The currently developed fluorescent RT-PCR multiple detection kit for the sheep respiratory tract syndromes completely meets the development requirement, the specificity is high, the sensitivity is high, and the lowest detection limit can reach 10 Copi / mu L or below.

The invention relates to an influenza virus detection test strip based on cellulose nanospheres and a preparation method thereof, and belongs to the technical field of immunodetection. Cellulose nanospheres are used for replacing a traditional colloidal gold material and used for preparing the immunochromatography test strip, a sample pad, a combination pad, a coating film and a water absorption pad are sequentially formed on a bottom plate from a sample adding end, an influenza A virus detection line and an influenza B virus detection line are arranged on the coating film, and the water absorption pad is arranged on the bottom plate. The test strip provided by the invention is applied to detection of influenza A and B virus antigens, the detection limit is as low as 0.05 ng / mL and 0.25 ng / mL, the detection test strip has lower detection limit and better specificity due to the use of the cellulose nanospheres, the preparation process is simplified, the stability of the test strip is also improved, and the test strip has wide application prospects.

The invention discloses a ransomware detection method and device, a storage medium and a computer program product, and relates to the technical field of computers.The method comprises the steps that a target file directory needing to be monitored is determined, and file type distribution information of the target file directory is obtained; wherein the file type distribution information comprises the types of the files contained in the target file directory and the distribution proportion of each type of files; and acquiring a bait file, and spraying the bait file to the target file directory based on the file type distribution information to realize detection of the ransomware virus. According to the method, the file type distribution information of the target file directory is dynamically acquired, and the bait file is accurately sprayed to the target directory according to the file type distribution information, so that the type and the distribution proportion of the bait file are highly consistent with the real file environment of the user system, the concealment of the bait file is improved, and the user experience is improved. Ransomware identification is avoided, bait files are avoided, and the accuracy and reliability of ransomware detection are enhanced.

The invention discloses a junk mail detection method and system and terminal equipment, and the method comprises the steps: carrying out the text recognition and extraction of uploaded mail data according to a data type, and obtaining to-be-detected information; performing anti-spam detection, address detection and anti-virus detection on different contents of the to-be-detected information to obtain a mail analysis log; and inputting the cut mail analysis log into a preset language analysis model to interpret a mail detection result, and generating a risk judgment result of the mail analysis log and a corresponding mail processing suggestion. According to the method, the junk mail can be identified by uploading the original mail or the image, the mail detection result and related processing suggestions which are more convenient for the user to understand are generated, and the use experience of the user is improved.

The invention discloses a CRISPR-Cas12a (clustered regularly interspaced short palindromic repeats-associated 12a) system for visually detecting bovine viral diarrhea viruses and a detection method, and belongs to the technical field of virus detection. According to the invention, RT-CPA pre-amplification is combined with Cas12a signal amplification by integrating reverse transcription cross primer amplification (RT-CPA) and targeted cutting capability of CRISPR / Cas12a, so that a bovine viral diarrhea virus detection platform is successfully constructed, and the sensitivity and specificity of BVDV detection are remarkably improved. Compared with traditional RT-PCR, the RT-CPA technology has the advantages that reaction conditions are remarkably simplified through isothermal amplification, dependence of a thermal cycler is avoided, meanwhile, amplification efficiency is improved through cross primer design, and the lower detection limit reaches 8.0 copies / microliter.

The invention relates to the technical field of virus detection, in particular to a method for enhancing animal virus detection sensitivity through optical fiber SPR probe surface modification, which comprises the following steps: S1, optical fiber SPR probe surface pretreatment: obtaining a hydroxylated surface; s2, gradient nanostructure modification: forming a gradient aperture surface topological structure; s3, carboxyl functionalization: immersing the substrate into a 3-aminopropyltriethoxysilane / methacrylic acid mixed solution, and constructing a carboxyl activation layer on the surface; s4, fixation of the aptamer: realizing directional fixation of the aptamer through EDC / NHS mediated carboxyl-amino coupling reaction; s5, non-specific site closing: carrying out cross-linking closing on the non-binding sites by adopting a thioacetamide / polydopamine composite solution; s6, virus detection: acquiring the surface refractive index variation through a phase modulation type SPR detection system; according to the invention, accurate fitting and quantitative correction of virus concentration are realized, and the stability and accuracy of detection are improved.

The invention relates to the field of biological detection, and discloses a detection model determination method based on a ResNeXt-50 network structure. The detection model determination method based on the ResNeXt-50 network structure comprises the following steps: grouping all acquired sample images for virus detection to obtain at least one group of verification sets and training sets corresponding to each group of verification sets; based on the ResNeXt-50 network structure, constructing a preset model; respectively inputting each group of training sets into a preset model for training to obtain at least one trained preset model; respectively inputting each group of verification sets into the trained preset models, and respectively verifying the performance of each trained preset model; and determining the trained preset model with the best performance as a target virus detection model. The target virus detection model is obtained through training, then the target virus detection model can detect the target virus, the virus detection result does not need to be manually determined, and the efficiency of determining the virus detection result is improved.

The invention provides a loop-mediated isothermal amplification primer composition for combined detection of multiple equine animal viruses, a disposable centrifugal micro-fluidic chip, an LAMP micro-fluidic chip kit and a nucleic acid detection method of the LAMP micro-fluidic chip kit. The loop-mediated isothermal amplification primer composition comprises nucleotide sequences as shown in SEQ ID: 1 to SEQ ID: 16. A fluorescent dye and a loop-mediated isothermal amplification primer composition are pre-embedded in the disposable centrifugal micro-fluidic chip. The LAMP micro-fluidic chip kit comprises a disposable centrifugal micro-fluidic chip and an LAMP amplification working solution. The problem of multiple detection of VSV, NiV, EEEV and WEEV in equine animal viruses is solved, and the detection method is high in specificity, short in reaction time, safe, simple and convenient to operate, high in sensitivity, low in cost and suitable for being used when a temporary virus detection laboratory is established.

The invention relates to the technical field of avian virus detection, and particularly discloses a primer probe combination, a kit and a method for simultaneously detecting chicken infectious anemia virus and chicken circle virus type 3 based on fluorescent RAA and application. The method can be used for simultaneously detecting the chicken infectious anemia virus and the chicken circle virus type 3, and has the advantages of simplicity in operation, short detection time, good specificity, high sensitivity, no need of expensive instruments and equipment, support of on-site rapid detection, suitability for large-scale popularization and application and the like; and effective technical support can be provided for rapid detection and screening of the chicken infectious anemia virus and the chicken circle virus type 3.