479 results about "Virus detection" patented technology

The invention discloses a method for detecting dengue virus based on RPA / RAA / ERA / MIRA-CRISPR / Cas12a, which comprises the following steps: extracting nucleic acid to obtain a sample to be detected, putting the extracted sample in a sterile and disinfected sample container, contacting the sample to be detected with a composition, a product combination, a reagent, a kit, a system or a system, determining the signal change after the sample to be detected is contacted with different substances, and determining the detection result of the dengue virus based on RPA / RAA / ERA / MIRA-CRISPR / Cas12a. In the same container, a to-be-detected sample is in contact with a composition, a product combination, a reagent, a kit, a system or a part used for RPA / RAA / ERA / MIRA nucleic acid amplification in the system, and then is in contact with a part used for CRISPR nucleic acid detection, the RPA / RAA / ERA / MIRA and CRISPR / Cas12a detection methods are combined, a rapid, simple, sensitive and specific dengue virus detection method is realized, the detection sensitivity is high, and the detection time is short. The reaction conditions of virus detection are further optimized.

The invention belongs to the field of virus detection and diagnosis, and relates to an anti-AAV2 monoclonal antibody as well as a preparation method and application thereof. The invention provides an anti-AAV2 rabbit monoclonal antibody, and amino acid sequences of a heavy chain variable region and a light chain variable region of the anti-AAV2 rabbit monoclonal antibody. The anti-AAV2 rabbit monoclonal antibody provided by the invention can be specifically combined with the VLP of AAV2, and can be used for detecting the AAV virus antigen. The anti-AAV2 monoclonal antibody provided by the invention provides an effective detection tool for the detection of AAV2 in the research and development process of a gene therapy vector and the detection of AAV2 in basic scientific research.

The invention provides a ransomware classification method and system based on a large model, and the method comprises the steps: carrying out the feature learning and extraction of preprocessed data through a deep learning large model, and obtaining advanced feature representation, and the deep learning large model is a large model which is subjected to the unsupervised or semi-supervised pre-training, and then is subjected to the fine adjustment based on the feature corpus fine adjustment of SFT supervision; performing preliminary classification and refined classification on the ransomware virus according to the advanced feature representation; and dynamically adjusting model parameters according to the novel ransomware sample. According to the method, by introducing a large model technology, limitation and defects in an existing ransomware detection and classification method are overcome, efficient detection and accurate classification of diversified ransomware viruses are achieved, the generalization ability of the model is enhanced, resource consumption is reduced, and usability and applicability of the system are improved; and the method is suitable for a large-scale data environment and quickly evolved ransomware ecology. According to the method, the overall level of network security defense is remarkably improved, and a more reliable and efficient ransomware virus protection scheme is provided for users.

The embodiment of the invention discloses a terahertz virus spectrum classification method and system based on transfer learning, and the method comprises the steps: obtaining a source domain terahertz spectrum data set containing various biomolecule hydrogen bond vibrations, and obtaining an initial feature extraction model; extracting a hydrogen bond vibration characteristic mode in a preset frequency band range, and taking a characteristic center of the hydrogen bond vibration characteristic mode as a cross-domain bridging characteristic; obtaining target sample data, wherein the sample data is a small sample data set; converting the similarity between the feature center and a target sample spectrum peak into a dynamic weight of a migration loss function, and performing layer-by-layer fine adjustment on the initial feature extraction model network to obtain a virus classification model; the fine tuning process comprises the following steps: adjusting a weight factor in real time according to the spectrum peak offset of the current batch of samples; inputting the terahertz spectrum data of the to-be-detected virus into the virus classification model to obtain a classification result of the to-be-detected virus. According to the application, the accuracy, robustness and industrial applicability of terahertz virus detection are remarkably improved.

The invention discloses a panda-derived feline panleucopenia virus RAA-CRISPRCas12a detection method, and belongs to the technical field of virus detection, and the panda-derived feline panleucopenia virus RAA-CRISPRCas12a detection method comprises the following steps: S1, primer and probe design; s2, screening an RAA amplification primer; s3, RAA reaction condition optimization; s4, performing RAA sensitivity and specificity verification; s5, preparing crRNA (Complementary Ribonucleic Acid); s6, optimal crRNA screening is carried out; s7, optimizing the RAA-CRISPR / Cas12a (Reverse Amplified Architecture- S8, the specificity and the sensitivity of the RAA-CRISPR / Cas12a are verified; s9, clinical sample detection; according to the invention, by elaborately designing a primer and a probe aiming at the capsid protein VP2 gene of the feline parvovirus (FPV), fully utilizing the advantages of recombinase-mediated amplification (RAA) and a CRISPR / Cas12a system, and comprehensively optimizing reaction conditions and components of the system, the detection method has remarkable advantages, can quickly complete the detection process under the condition of constant temperature of 37 DEG C, is extremely high in sensitivity, and can be used for rapidly detecting the feline parvovirus. The lowest detection limit can reach 1 * 10 < 1 > copies / mu L, the specificity is high, and the coincidence rate with a real-time fluorescent quantitative PCR method is as high as 100%.

The invention discloses an integrated remote teaching assistance and management system for an education scene, belongs to the field of education informatization, and solves the problems that a traditional education system is split in function, tedious in process, high in safety risk and the like. The system integrates three modules: 1) remote call broadcast: supporting home / school / student real-time broadcast notification (including accessory poison check); 2) remote examination and approval: processing and configuring a multi-level examination and approval process, performing state synchronization and preventing tampering of block chain evidence storage; and 3) auxiliary teaching: supporting functions of courseware uploading, homework arrangement, statistics and the like. The data is stored in a relational database (such as MySQL), and the full-life-cycle security of the data is guaranteed through multi-factor authentication, RBAC permission control, high-strength encryption and block chain evidence storage. The beneficial effects are that function integration improves efficiency; security is enhanced through multi-factor authentication and the block chain; an approval specification process can be configured; visual report auxiliary decision making; a high-availability technology (such as WebSocket heartbeat) is guaranteed to be stable, and a whole-process digital and safe teaching management scheme is provided for schools.

The invention discloses an intelligence management method and system based on network security, and relates to the technical field of network security, and the method comprises the steps: setting a firewall between a client network and a server network; performing encryption processing on the key data by using a key encryption algorithm; carrying out virus detection and intrusion detection, and applying an intrusion detection technology and vulnerability scanning in a server network; user identity verification is carried out, a user login process is designed, and login operation of the user is controlled and managed; tracking and positioning the source of the security problem; corresponding server network data processing authority is given to the user; double servers and a disk array are adopted for data storage, and the data are automatically backed up every preset backup cycle. The information collection module and the information management module are arranged to effectively block illegal invasion and virus spreading programs, so that the safety of user information and information is protected, and the confidentiality of the information in the transmission and storage process is ensured.

The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus, and belongs to the technical field of genetic engineering. The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus. The pseudovirus contains a nucleic acid fragment of a Nipah virus N gene of which the nucleotide sequence is shown as SEQ ID NO.1. The invention further provides a preparation method of the pseudovirus. The pseudovirus of the Nipah virus can be used as a ribonucleic acid standard substance of the pseudovirus of the Nipah virus, and is applied to the aspects of quantity transmission, calibration, instrument testing, evaluation and control of a testing method, quality verification, quality control, production process detection and the like of a laboratory related to Nipah virus detection. The pseudovirus of the Nipah virus, as the ribonucleic acid standard substance of the pseudovirus of the Nipah virus, has the following advantages of accurate fixed value, good uniformity, stable quantity value and traceability, and can promote the improvement of the detection capability and the technical level of a national Nipah detection laboratory.

The invention relates to the technical field of image processing, and discloses an improved StarNet-YOLOv13-based unmanned aerial vehicle field tobacco virus disease lightweight detection method, which comprises the following steps: acquiring a to-be-detected image of a field tobacco plant, and then inputting the to-be-detected image into a trained tobacco virus detection model to obtain a detection result of the tobacco plant; wherein the tobacco virus detection model is obtained by replacing a backbone network with a star network, replacing a C3K2 module in a neck network with a feature fusion module and replacing a detection head with a DetectMBConv detection head in a YOLOv13 model; the feature fusion module is constructed by introducing a partial convolution mechanism into the C3K2 module; thus, through the star network (StarNet), the feature fusion module (DSC3k2PConv module) and the DetectMBConv detection head, the small target scab detection capability and the complex scene robustness can be improved while the parameter and calculation overhead can be reduced, so that the tobacco virus detection model can meet the deployment requirement of the computing power limited equipment side, such as an unmanned aerial vehicle, and the detection precision is ensured at the same time.

The invention relates to the technical field of sweet potato virus detection, and provides a sweet potato virus G detection method. The invention relates to a sweet potato virus G specific primer pair. The nucleotide sequence of a forward primer SPVG-F of the specific primer pair is as shown in SEQ ID NO: 1; and the nucleotide sequence of the reverse primer SPVG-R is as shown in SEQ ID NO: 2. According to the technical scheme, the problems of low detection sensitivity and low detection efficiency when a sweet potato G virus amplification primer and a sweet potato G virus same family virus compositely infect a sample in the prior art are solved.

The invention relates to the technical field of virus detection, and particularly discloses a kit for detecting African swine fever virus by using a fluorescent probe method LAMP (loop-mediated isothermal amplification), the kit for detecting African swine fever virus by using the fluorescent probe method LAMP comprises an LAMP reaction solution for detecting African swine fever virus, a specific fluorescent probe and African swine fever virus genome DNA (deoxyribonucleic acid); the LAMP reaction liquid comprises a specific primer combination consisting of a pair of specific outer primers, a pair of specific inner primers and a loop primer; the detection method of the kit comprises the following steps: nucleic acid extraction, fluorescent probe LAMP reaction system preparation, sample amplification and result determination. The kit for detecting the African swine fever virus by using the fluorescent probe method LAMP is simple and convenient to operate, has the advantages of strong specificity, high sensitivity, short time consumption and the like, can efficiently detect the African swine fever virus, and has important application value in prevention and control of African swine fever epidemic situation.

The invention discloses a kit for simultaneously detecting bluetongue virus, foot and mouth disease virus, schmallenberg virus, peste des petits ruminants virus and sheep pox virus, and belongs to the technical field of virus detection. The kit comprises primers as shown in SEQ ID NO. 1 to SEQ ID NO. 18 and a probe as shown in SEQ ID NO. 18. After a single system of bluetongue virus, foot-and-mouth disease virus, schmallenberg virus, peste des petits ruminants virus and capripox virus is tested, a sextuple system containing an interior label is established, and through testing of indexes such as linearity, minimum detection limit, specificity, precision and repeatability, NTC repetition, comparison of multiple fluorescent RT-PCR and single fluorescent RT-PCR, the kit can be used for detecting the virus resistance of the peste des petits ruminants virus and the capripox virus. The currently developed fluorescent RT-PCR multiple detection kit for the sheep respiratory tract syndromes completely meets the development requirement, the specificity is high, the sensitivity is high, and the lowest detection limit can reach 10 Copi / mu L or below.

The invention discloses a ransomware detection method and device, a storage medium and a computer program product, and relates to the technical field of computers.The method comprises the steps that a target file directory needing to be monitored is determined, and file type distribution information of the target file directory is obtained; wherein the file type distribution information comprises the types of the files contained in the target file directory and the distribution proportion of each type of files; and acquiring a bait file, and spraying the bait file to the target file directory based on the file type distribution information to realize detection of the ransomware virus. According to the method, the file type distribution information of the target file directory is dynamically acquired, and the bait file is accurately sprayed to the target directory according to the file type distribution information, so that the type and the distribution proportion of the bait file are highly consistent with the real file environment of the user system, the concealment of the bait file is improved, and the user experience is improved. Ransomware identification is avoided, bait files are avoided, and the accuracy and reliability of ransomware detection are enhanced.

The invention discloses a junk mail detection method and system and terminal equipment, and the method comprises the steps: carrying out the text recognition and extraction of uploaded mail data according to a data type, and obtaining to-be-detected information; performing anti-spam detection, address detection and anti-virus detection on different contents of the to-be-detected information to obtain a mail analysis log; and inputting the cut mail analysis log into a preset language analysis model to interpret a mail detection result, and generating a risk judgment result of the mail analysis log and a corresponding mail processing suggestion. According to the method, the junk mail can be identified by uploading the original mail or the image, the mail detection result and related processing suggestions which are more convenient for the user to understand are generated, and the use experience of the user is improved.

The invention discloses a CRISPR-Cas12a (clustered regularly interspaced short palindromic repeats-associated 12a) system for visually detecting bovine viral diarrhea viruses and a detection method, and belongs to the technical field of virus detection. According to the invention, RT-CPA pre-amplification is combined with Cas12a signal amplification by integrating reverse transcription cross primer amplification (RT-CPA) and targeted cutting capability of CRISPR / Cas12a, so that a bovine viral diarrhea virus detection platform is successfully constructed, and the sensitivity and specificity of BVDV detection are remarkably improved. Compared with traditional RT-PCR, the RT-CPA technology has the advantages that reaction conditions are remarkably simplified through isothermal amplification, dependence of a thermal cycler is avoided, meanwhile, amplification efficiency is improved through cross primer design, and the lower detection limit reaches 8.0 copies / microliter.

The invention relates to the technical field of virus detection, in particular to a method for enhancing animal virus detection sensitivity through optical fiber SPR probe surface modification, which comprises the following steps: S1, optical fiber SPR probe surface pretreatment: obtaining a hydroxylated surface; s2, gradient nanostructure modification: forming a gradient aperture surface topological structure; s3, carboxyl functionalization: immersing the substrate into a 3-aminopropyltriethoxysilane / methacrylic acid mixed solution, and constructing a carboxyl activation layer on the surface; s4, fixation of the aptamer: realizing directional fixation of the aptamer through EDC / NHS mediated carboxyl-amino coupling reaction; s5, non-specific site closing: carrying out cross-linking closing on the non-binding sites by adopting a thioacetamide / polydopamine composite solution; s6, virus detection: acquiring the surface refractive index variation through a phase modulation type SPR detection system; according to the invention, accurate fitting and quantitative correction of virus concentration are realized, and the stability and accuracy of detection are improved.

The invention relates to the field of biological detection, and discloses a detection model determination method based on a ResNeXt-50 network structure. The detection model determination method based on the ResNeXt-50 network structure comprises the following steps: grouping all acquired sample images for virus detection to obtain at least one group of verification sets and training sets corresponding to each group of verification sets; based on the ResNeXt-50 network structure, constructing a preset model; respectively inputting each group of training sets into a preset model for training to obtain at least one trained preset model; respectively inputting each group of verification sets into the trained preset models, and respectively verifying the performance of each trained preset model; and determining the trained preset model with the best performance as a target virus detection model. The target virus detection model is obtained through training, then the target virus detection model can detect the target virus, the virus detection result does not need to be manually determined, and the efficiency of determining the virus detection result is improved.

The invention relates to the technical field of avian virus detection, and particularly discloses a primer probe combination, a kit and a method for simultaneously detecting chicken infectious anemia virus and chicken circle virus type 3 based on fluorescent RAA and application. The method can be used for simultaneously detecting the chicken infectious anemia virus and the chicken circle virus type 3, and has the advantages of simplicity in operation, short detection time, good specificity, high sensitivity, no need of expensive instruments and equipment, support of on-site rapid detection, suitability for large-scale popularization and application and the like; and effective technical support can be provided for rapid detection and screening of the chicken infectious anemia virus and the chicken circle virus type 3.

The embodiment of the invention provides a virus interception method and device, electronic equipment and a storage medium. The method comprises the steps of starting a target drive program in an operating system in response to a starting instruction of the operating system; acquiring a rule set by the target driver, wherein the rule set comprises a plurality of virus rules; in the process of loading the starting drive program, obtaining first program information of the starting drive program by the target drive program; the target driving program performs virus identification on the starting driving program based on the first program information of the starting driving program and the rule set to obtain a virus identification result; and if the virus identification result indicates that the starting drive program is the virus, intercepting the starting drive program. By the adoption of the method, virus detection can be carried out before the starting drive program is started, interception is carried out when it is detected and confirmed that the starting drive program is the virus program, and therefore the safety of an operating system is improved.

The invention discloses a primer group for identifying six different genotypes of IBV epidemic strains and a high-resolution melting curve detection method, and relates to the technical field of virus detection. The primer group for identifying the six IBV epidemic strains with different genotypes is HRM-F / HRM-R, and the nucleotide sequences of the primer group are shown as SEQ ID NO. 1 and SEQ ID NO. 2. By utilizing the specific primer group and the detection method disclosed by the invention, qualitative and quantitative analysis on single infection or mixed infection of IBV epidemic strain genotypes of LX4 (GI-19), Mass (GI-I), 4 / 91 (GI-13), LDT3-A (GI-28), Taiwan (GI-7) and CK / CH / LSC / 99I (GI-22) can be realized. The detection sensitivity is high, the specificity is good, the accuracy is high, the operation is simple and rapid, the cost is low, and the method is suitable for popularization.

This application relates to the field of virus detection technology, specifically disclosing a real-time fluorescent PCR detection kit and method for H10N7 avian influenza virus. The kit includes sample lysis extract, sample washing solution I, sample washing solution II, and reaction premix for detecting H10N7 subtype avian influenza virus; the detection method is an integrated detection method. This application achieves simultaneous detection of H10 and N7 subtype AIVs by designing specific primers and probes. The kit prepared using these primers and probes not only improves detection efficiency but also significantly enhances sensitivity and specificity. The combined use of MAOPA technology and real-time quantitative PCR technology further improves the accuracy of the detection results, making this kit of this application of significant application value in avian influenza prevention and control.

The invention discloses a PCR (Polymerase Chain Reaction) primer for amplifying a reverse transcription product of dengue virus, a PCR kit and a method for detecting the dengue virus, and belongs to the technical field of virus detection. Comprising an upstream primer DENV-F as shown in SEQ ID NO. 1 and a downstream primer DENV-R as shown in SEQ ID NO. 2. The dengue virus detection method based on the PCR amplification reaction, the three-generation sequencing and the phylogenetic analysis is established by utilizing the characteristics that the PCR reaction is efficient, rapid and accurate, the three-generation sequencing is rapid, the full-length sequencing of about 10,000 basic groups can be provided, one sequencing result represents one complete virus sequence, the phylogenetic analysis is accurate in strain analysis and the like. The kit plays a role in rapid and accurate detection of dengue viruses. The PCR primer used in the invention has high sensitivity, can reach 0.01 pg / reaction, has good specificity, and does not react with other sequences while amplifying the dengue virus.

The invention belongs to the technical field of virus detection, and discloses a digital PCR (polymerase chain reaction) kit for simultaneously detecting African swine fever virus, porcine reproductive and respiratory syndrome virus and swine fever virus, and the kit comprises primers and probes as shown in SEQ ID NO.1-SEQ ID NO.24. After a single system of an African swine fever virus-VP72 gene, an African swine fever virus-CD2V gene, an African swine fever virus-14L gene, a porcine reproductive and respiratory syndrome virus-RdRP gene, a porcine reproductive and respiratory syndrome virus-European strain, a porcine reproductive and respiratory syndrome virus-American strain, a swine fever virus-vaccine strain and a swine fever virus-wild strain is tested, a nine-fold digital PCR system containing an internal standard is established; through tests of indexes such as linearity, specificity, minimum detection limit, repeatability, comparison of a single digital PCR system and a multiple digital PCR system, the currently developed digital PCR multiple detection kit completely meets development requirements and is high in specificity and sensitivity, and the minimum detection limit of the digital PCR multiple detection kit can reach 10 Copies / mu L or below.

The invention relates to a primer for visual detection of ToBRFV and application thereof, and belongs to the technical field of plant virus detection, the primer comprises an upstream outer primer ToBRFV-F3 with a nucleotide sequence as shown in SEQ ID No.2, a downstream outer primer ToBRFV-B3 with a nucleotide sequence as shown in SEQ ID No.3, an upstream inner primer ToBRFV-FIP with a nucleotide sequence as shown in SEQ ID No.4, and a downstream inner primer ToBRFV-BIP with a nucleotide sequence as shown in SEQ ID No.5. In addition, the invention also comprises the application of the primer in the visual detection of the ToBRFV based on LAMP-CRISPR / Cas12a. The method is sensitive and accurate in ToBRFV detection, and has the advantages of easiness in operation, rapidness in detection, high specificity, high sensitivity, visualization and the like.

The invention provides a probe group and a kit for capturing a full-length genome of a respiratory syncytial virus (RSV), and belongs to the technical field of virus detection. The invention discloses a probe group for capturing a full-length genome of a respiratory syncytial virus. The probe group comprises probes with nucleotide sequences as shown in SEQ ID NO: 1-SEQ ID NO: 900. According to the invention, the probe group and next-generation sequencing are combined to obtain an RSV full-length genome sequence. Compared with a primer or a probe used in a sequencing method in the prior art, the single probe in the probe group is short and high in specificity, the same site is designed by using the imbricate probe, all variations of the site are covered, and when a virus sequence is captured, the probe group has the characteristics of high sensitivity, high coverage rate and high flux.

The invention relates to the technical field of plant virus detection, in particular to a system and a method for detecting areca yellowing related viruses based on an RPA-LFD technology. The primer group for detecting the areca yellows virus APV1 is characterized by comprising a specific primer pair, the nucleotide sequence of an upstream primer in the specific primer pair is as shown in SEQ ID No.1 in a sequence table, and the nucleotide sequence of a downstream primer is as shown in SEQ ID No.2 in the sequence table; the device further comprises a probe. The probe is labeled and modified by biotin or FAM fluorophore; the nucleotide sequence of the modified probe is as shown in SEQ ID No.3 in the sequence table. The kit has the advantages that the kit has higher specificity and lower detection limit in the aspect of detection, and has no cross reaction with other viruses such as areca necrotic ringspot virus; the primer probe combination is suitable for RPA-LFD detection, and the time required by an amplification reaction is short; the reaction result is shown on the test strip and can be observed by naked eyes.

The unmanned aerial vehicle underwater virus detection and concentration device comprises an unmanned aerial vehicle body, a lighting device is arranged in the middle of the upper end of the unmanned aerial vehicle body, wings are arranged on the four faces of the upper end of the unmanned aerial vehicle body, a first motor is arranged in the unmanned aerial vehicle body, and a rotating shaft is arranged on a driving shaft of the first motor; a cable fixed to the shell is wound on the rotating shaft, a groove matched with the shell is formed in the bottom of the unmanned aerial vehicle body, the lower end of the shell is connected with an assembling structure, and the lower end of the assembling structure is fixedly connected with a water taking structure; the assembly structure is used for accommodating a water sample and simultaneously implementing a virus concentration process; the water taking structure is used for taking water and sampling the water body. The device has the characteristics of safety and high convenience, and can realize quick fixed-point sampling and virus concentration in a water area.

The present invention discloses a method for visual detection of Tomato leaf curl New Delhi virus, belonging to the plant virus detection technology in the field of biotechnology. First, the genomic DNA extracted from the plant sample to be detected is added to the RAA reaction system containing RAA primers and subjected to isothermal amplification; then the isothermal amplification product is added to the CRISPR / Cas12a detection system containing specific crRNA and ssDNA fluorescent probes for reaction; finally, the fluorescence signal of the reaction product is detected. If the fluorescence signal is positive, it indicates that Tomato leaf curl New Delhi virus is detected in the plant sample, otherwise it indicates that the virus is not detected. The present invention has the advantages of good specificity, short detection time, high sensitivity, and does not rely on expensive instruments, etc., and can accurately detect through instruments or directly make a preliminary judgment of the results in the field with limited conditions.

The invention provides a Marburg virus detection standard substance as well as a preparation system and application thereof, and belongs to the technical field of biological detection. The armored RNA standard substance containing the Marburg virus NP gene segment, provided by the invention, contains the NP gene segment, can be used for nucleic acid detection of the Marburg virus taking the NP gene as a target spot, and is used for quality control of detection methods and reagents such as common PCR (Polymerase Chain Reaction), fluorescent quantitative PCR, digital PCR, LAMP (Loop-Mediated Isothermal Amplification) and the like. The armored RNA standard substance provided by the invention has the advantages of high purity, no plasmid DNA residue and good quality control performance. The armored RNA virus-like particle simulates the structure of a natural virus, and can participate in links such as nucleic acid extraction and amplification as an animal body or human body blood sample containing Marburg virus, so that the whole-course quality control of the whole detection process is realized, and the authenticity and reliability of the detection result are ensured.

The invention provides an RPA-PAND detection kit and detection method for canine parvovirus and canine adenovirus, and belongs to the technical field of canine virus detection.The kit comprises PfAgo protein and a gDNA and MB combination used for detecting canine parvovirus MVC, canine parvovirus CPV and canine adenovirus CAV. The RPA-PAND rapid nucleic acid detection method for detecting canine parvoviruses MVC and CPV and canine adenovirus CAV is successfully established on the basis of PfAgo protein mediated target detection and in combination with the RPA technology, and the method has the advantages of high sensitivity, high specificity, low cost, relatively low position targeting limitation, simple and convenient reaction system, no aerosol pollution, capability of performing multi-channel nucleic acid detection and the like; and a new reagent and a new way are provided for clinical diagnosis and epidemiological monitoring of viruses MVC, CPV and CAV.