229 results about "Virus detection" patented technology

The embodiment of the invention discloses a terahertz virus spectrum classification method and system based on transfer learning, and the method comprises the steps: obtaining a source domain terahertz spectrum data set containing various biomolecule hydrogen bond vibrations, and obtaining an initial feature extraction model; extracting a hydrogen bond vibration characteristic mode in a preset frequency band range, and taking a characteristic center of the hydrogen bond vibration characteristic mode as a cross-domain bridging characteristic; obtaining target sample data, wherein the sample data is a small sample data set; converting the similarity between the feature center and a target sample spectrum peak into a dynamic weight of a migration loss function, and performing layer-by-layer fine adjustment on the initial feature extraction model network to obtain a virus classification model; the fine tuning process comprises the following steps: adjusting a weight factor in real time according to the spectrum peak offset of the current batch of samples; inputting the terahertz spectrum data of the to-be-detected virus into the virus classification model to obtain a classification result of the to-be-detected virus. According to the application, the accuracy, robustness and industrial applicability of terahertz virus detection are remarkably improved.

The invention relates to the technical field of image processing, and discloses an improved StarNet-YOLOv13-based unmanned aerial vehicle field tobacco virus disease lightweight detection method, which comprises the following steps: acquiring a to-be-detected image of a field tobacco plant, and then inputting the to-be-detected image into a trained tobacco virus detection model to obtain a detection result of the tobacco plant; wherein the tobacco virus detection model is obtained by replacing a backbone network with a star network, replacing a C3K2 module in a neck network with a feature fusion module and replacing a detection head with a DetectMBConv detection head in a YOLOv13 model; the feature fusion module is constructed by introducing a partial convolution mechanism into the C3K2 module; thus, through the star network (StarNet), the feature fusion module (DSC3k2PConv module) and the DetectMBConv detection head, the small target scab detection capability and the complex scene robustness can be improved while the parameter and calculation overhead can be reduced, so that the tobacco virus detection model can meet the deployment requirement of the computing power limited equipment side, such as an unmanned aerial vehicle, and the detection precision is ensured at the same time.

The invention provides an RPA-PAND detection kit and detection method for canine parvovirus and canine adenovirus, and belongs to the technical field of canine virus detection.The kit comprises PfAgo protein and a gDNA and MB combination used for detecting canine parvovirus MVC, canine parvovirus CPV and canine adenovirus CAV. The RPA-PAND rapid nucleic acid detection method for detecting canine parvoviruses MVC and CPV and canine adenovirus CAV is successfully established on the basis of PfAgo protein mediated target detection and in combination with the RPA technology, and the method has the advantages of high sensitivity, high specificity, low cost, relatively low position targeting limitation, simple and convenient reaction system, no aerosol pollution, capability of performing multi-channel nucleic acid detection and the like; and a new reagent and a new way are provided for clinical diagnosis and epidemiological monitoring of viruses MVC, CPV and CAV.

The invention relates to the technical field of virus detection, and discloses a method and a kit for detecting Chinese sacbrood virus. The method comprises: firstly, carrying out isothermal amplification on a CSBV specific target sequence in a sample nucleic acid by using an RPA technology, the RPA technology adopting a specific primer pair, the sequences of which are SEQ ID NO: 4 and SEQ ID NO: 5; then, an amplification product is mixed with a CRISPR / Cas12b detection system, and the system comprises Cas12b protein, specific sgRNA with the sequence shown as SEQ ID NO: 2 and a single-chain DNA reporter probe marked by fluorescence; when a CSBV target exists, the activated Cas12b protein trans-cleavage reporter probe generates a detectable fluorescence signal, so that rapid and high-sensitivity detection of the CSBV is realized. The invention further provides a detection kit suitable for the method. The detection kit comprises the primer pair, sgRNA and Cas12b protein. When the CSBV is detected based on the method and the kit, complex instruments are not needed, the operation is simple and convenient, the detection time is shorter than 1 hour, the sensitivity and the specificity are high, and the method and the kit are suitable for onsite rapid detection in a bee field.

The invention belongs to the technical field of virus detection, and particularly relates to a primer probe composition for detecting Japanese encephalitis virus and application, the primer probe composition comprises a probe, an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is shown as SEQ ID No.3, and the nucleotide sequence of the downstream primer is shown as SEQ ID No.6. According to the primer probe composition, the invention provides a non-diagnostic method for detecting the Japanese encephalitis virus, and the method has the advantages of rapidness, real time, strong specificity, high sensitivity, simplicity and convenience in operation and suitability for various fluorescence detection equipment, and can accurately detect the Japanese encephalitis virus from a sample to be detected.

The invention relates to the technical field of antibodies, and discloses an antibody for specifically recognizing porcine pseudorabies virus gB (507-734 aa) or an antigen binding fragment thereof and application. The antibody or the antigen binding fragment thereof comprises a heavy chain and a light chain; the antibody or the antigen binding fragment thereof can be used for detecting the existence or the level of the porcine pseudorabies virus gB protein in a sample, and has key significance on optimization of a porcine pseudorabies virus detection method, improvement of diagnosis accuracy and realization of effective prevention and control of epidemic situations.

The application discloses a primer probe set for simultaneously detecting multiple frog viruses, a kit and application thereof, and belongs to the technical field of animal virus detection.The primer probe set for detecting frog viruses comprises a primer group with the sequence shown in SEQ ID NO.1-2 and a probe with the sequence shown in SEQ ID NO.3.According to the D5family NTPase gene sequence of the Ranavirus member, the primer and the probe are designed, and through optimization of reaction conditions, a method for detecting frog viruses based on qPCR is finally established.The method has the characteristics of high specificity, good repeatability, high sensitivity, simple operation and reliability, and has good market application prospect and popularization and application value.

The application discloses an EHNV virus detection method and system based on dynamic fluorescence intensity monitoring. The method divides the water area to be detected into small areas, obtains water impurity information, carries out fluorescence detection under different impurity characteristics, analyzes fluorescence data to evaluate the influence of impurities on detection accuracy. According to the accuracy influence data, the best sampling area is determined, and the EHNV virus sample is collected, the fluorescence intensity change of the sample is monitored, and finally the EHNV virus information in the water area is obtained. This method can improve the detection sensitivity and accuracy under the interference of impurities, and is suitable for the fields of aquaculture and ecological environment monitoring. The application also provides a corresponding detection system, which comprises a fluorescence detection device, a data analysis module and a sample processing unit, has automatic operation and efficient data processing capacity, and is helpful for preventing and controlling the spread of EHNV virus.

The invention belongs to the technical field of biological detection, and relates to a Chikungunya virus detection kit and a detection method. The kit is based on the principle of PCR detection and comprises a primer pair, the sequence of a forward primer of the primer pair is shown as SEQ ID NO: 1, and the sequence of a reverse primer of the primer pair is shown as SEQ ID NO: 2. The chikungunya virus detection kit and the detection method can realize rapid, sensitive and specific detection of chikungunya virus based on the principle of PCR detection, and are especially suitable for various application scenes such as clinical samples, human or animal samples, environmental samples, scientific research samples and the like.

The utility model belongs to virus storage equipment technical field, and disclose a kind of virus detection specimen storage device, including storage box, the outside hinged cover of storage box is equipped with, and the inside assembly of storage box is equipped with quick -mount structure, the outside assembly of cover is used for the abutment structure for limiting specimen movement, the quick -mount structure includes the fixed plate installed from top to bottom in the inner wall of storage box, by the design of quick -mount structure, push freezer to make it slide inside baffle, extrude first spring simultaneously, during this period, the slider outside first connecting rod slides along the trajectory of heart-shaped sliding slot, and slides into one place limit slot and is locked, at this time, freezer slides to the outside of storage box, the dry ice or ice bag in freezer can be replaced, after replacement is completed, freezer is pushed again, at this time, slider is separated from first limit slot, and along heart-shaped sliding slot is moved to second limit slot and is locked, freezer is reset, and the quick replacement of dry ice or ice bag etc. in freezer is further achieved.

The invention discloses a network virus detection method and system, a storage medium and electronic equipment, and belongs to the field of digital network security. The data network virus detection method comprises the following steps: deploying a plurality of file restoration nodes in each region of data network service in a distributed manner; and the file detection node is used for detecting the to-be-detected file. The detection processing comprises the following steps: dividing the to-be-detected file into a first-time detected file and a repeated detected file by utilizing a file buffer pool; carrying out full-quantity file detection on the first-time detected files, and updating the file buffer pool; detecting the repeated files to be detected based on a comparison rule version mode, and updating the file buffer pool; and outputting a detection result of the to-be-detected file. And the file buffer pool is configured with an automatic cleaning mechanism based on time and space. When the file detection rule is updated, the buffer pool cannot be emptied, and only newly added or modified rules need to be detected for the historically scanned file, so that the data processing amount is reduced, and the performance overhead of file detection is saved.

The utility model relates to the technical field of virus detection, in particular to an anti-pollution folding type portable virus detection box. The utility model provides an anti-pollution folding type portable virus detection box which can automatically limit the upper part of a virus storage box when a box cover is closed, and avoids virus sample leakage caused by accidental opening of the cover of the virus storage box. An anti-pollution folding portable virus detection box comprises a box body, a box cover, hinges and the like, and the upper rear portion of the box body is connected with the box cover through the left hinge and the right hinge. The box cover is rotationally closed, the reset spring gradually restores, the sliding frame is driven to move forwards, the pressing cover moves forwards, the pressing cover makes contact with the upper side of the virus storage box, the virus storage box is limited, and the effect that the upper portion of the virus storage box can be automatically limited when the box cover is closed is achieved; and virus sample leakage caused by accidental opening of the cover of the virus storage box is avoided.

The invention belongs to the technical field of virus detection, and particularly relates to a primer composition for detecting an avian influenza NA gene, a detection method and a detection device. The avian influenza NA genes comprise NA genes of an influenza A virus H5N1, an influenza A virus H5N6, an influenza A virus H7N7, an influenza A virus H7N9 and an influenza A virus H9N2, and the primer composition comprises nucleotide sequences as shown in SEQ ID No.1-25. The primer composition for detecting the NA gene causing the highly pathogenic human and livestock co-infected avian influenza, provided by the invention, can be used for detecting whether a sample contains the NA gene causing the highly pathogenic human and livestock co-infected avian influenza, namely NA genes of influenza A virus subtypes H5N1, H5N6, H7N7, H7N9 and H9N2, and a result can be conveniently, quickly and accurately detected; in a practical application process, a detection result can be obtained within 40 minutes.

This invention discloses an SFTSV virus detection kit and method based on LAMP-CRISPR / Cas combined with electrochemistry. By integrating multiple detection technologies, this invention combines the CRISPR / Cas system with LAMP isothermal amplification technology and electrochemical detection technology to achieve more efficient and sensitive nucleic acid detection, greatly shortening the detection time and meeting the needs of rapid clinical diagnosis. The detection exhibits high specificity and sensitivity, enabling accurate detection of samples with extremely low concentrations. The equipment is simplified and portable, facilitating real-time monitoring and automated operation. This invention innovatively combines real-time signal detection with automated operation, enabling real-time monitoring of the amplification process and direct acquisition of results through electrochemical signals, reducing operational difficulty and improving detection efficiency.

The application discloses a crRNA, a primer group, a detection system and a detection method of SARS-CoV-2, and belongs to the technical field of virus detection. The CRISPR-Cas13a detection system established by the application utilizes the non-specific cleavage activity of the Cas13a protein, combines with the recombination polymerase amplification technology which can efficiently amplify the target fragment, and the system comprises the Cas13a, the sgRNA corresponding to the target virus to be detected and the reporter RNA chain comprising a cleavage fluorescence; when the Cas13 protein recognizes the target RNA chain, the corresponding template is cleaved, then the collateral cleavage accessory activity thereof is activated, and then the reporter RNA is cleaved and the detectable fluorescence signal is released, so that the rapid, inexpensive and high-sensitivity detection of trace nucleic acid is realized, and the minimum detection limit for SARS-CoV-2 is 1 copy / muL.

The invention discloses a preparation method and application of an aptamer functionalized nano-enzyme probe for detecting H1N1 virus, and belongs to the technical field of biosensing and virus detection. The invention aims to solve the problem of limitation of the existing H1N1 virus detection method in sensitivity, specificity and stability. The preparation method comprises the following steps: 1, mixing and incubating a three-metal nickel-palladium-gold nano-enzyme solution containing a hollow structure and a sulfhydrylation H1N1 hemagglutinin protein specific recognition aptamer solution; and 2, sealing the surface. According to the application, the kit is used for detecting the H1N1 virus.

The invention relates to the technical field of virus detection, and particularly discloses a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit for identifying a classical swine fever virus vaccine strain and a wild strain. The kit comprises a specific primer combination, a specific fluorescent probe, a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) reaction solution, a positive control and a negative control, the primer combination comprises a vaccine strain and wild strain specific primer pair targeting a virus 5 '-UTR region and an E2 gene; the fluorescent probes comprise probes corresponding to the targets respectively and are marked with fluorophores; verification is carried out through double gene targets, missing detection caused by single site mutation is effectively avoided, and the method has the advantages of high specificity, high sensitivity and high accuracy; the invention further provides a non-diagnostic purpose detection method of the kit, detection can be completed within 2 h, and the kit is suitable for rapid identification, epidemic situation monitoring and purification effect evaluation of classical swine fever virus vaccine strains and wild strains in clinical samples.

This invention discloses a sample collection device for animal virus detection, relating to the field of virus collection technology. It includes a device housing with a rotating structure inside. The rotating structure includes a main shaft rotatably mounted inside the housing, a winding shaft fixedly connected to the upper end of the main shaft, and a sterilization structure on the outer surface of the winding shaft. The sterilization structure includes an adjusting rope wound around the outer surface of the winding shaft at one end, a connecting bracket fixedly connected to the other end of the adjusting rope, a sterilizing lamp on the outer surface of the connecting bracket, a guide wheel movably mounted on the outer surface of the adjusting rope, a fixed bracket between the guide wheel and the inner wall of the device housing, and a slide rail fixedly connected to the outer surface of the inner wall of the device housing. This structure solves the problems of existing animal virus detection sample collection devices where the support plate holding the collection bottle is fixed and cannot rotate, making it inconvenient to remove the collection bottle, and the sterilization lamp used for sterilization has a fixed position and poor sterilization effect.

The invention provides qRT-PCR (quantitative real-time polymerase chain reaction) primers, a kit and a detection method for detecting tomato brown wrinkled fruit virus with high sensitivity, and belongs to the technical field of plant virus detection. The qRT-PCR primer for detecting the tomato brown wrinkled fruit virus, disclosed by the invention, is as shown in SEQ ID NO. 1 and SEQ ID NO. 2. The invention also provides a qRT-PCR detection method of the tomato brown wrinkled fruit virus, the primer and the detection method provided by the invention have the characteristics of strong specificity and high sensitivity, the detection limit is 1.562 * 10 < 1 > copies / [mu] L, and the primer and the detection method have a better detection effect on a sample with low virus loading amount; and a technical support is provided for detecting the tomato brown wrinkled fruit virus and preventing and controlling the tomato brown wrinkled fruit virus.

The invention relates to the technical field related to immunological detection, in particular to a mouse anti-porcine epidemic diarrhea virus monoclonal antibody, an immunogen and application thereof. The amino acid sequences of complementary determining regions CDR1, CDR2 and CDR3 of a heavy chain variable region of the monoclonal antibody or the antigen binding fragment of the monoclonal antibody are respectively as follows: GFSLSTSGVV, RIDPSDSYT and ARDREKSDNSGYFDY, and the amino acid sequences of complementary determining regions CDR1, CDR2 and CDR3 of a light chain variable region of the monoclonal antibody or the antigen binding fragment of the monoclonal antibody are respectively as follows: RASKSISKYLA, EASSLES and LQHTPKKNK. The monoclonal antibody can be specifically combined with nucleocapsid proteins (N proteins) of all subtypes of the porcine epidemic diarrhea virus, and a porcine epidemic diarrhea virus detection kit prepared from the monoclonal antibody has the advantages of high sensitivity, strong specificity, wide detection range, short detection time and the like.

Provided is a novel isothermal gene amplification method which can easily detect RNA viruses at low cost in a short time. The isothermal gene amplification method (hereinafter, referred to as "amplification method") according to the present invention is an isothermal gene amplification method for a target sequence in an RNA genome, and comprises (A) an amplification reaction template generation step, (B) a DNA amplification step for increasing the amplification reaction template, and (C) an RNA amplification step.

The invention provides a qPCR reaction system, a product thereof and application of the qPCR reaction system in virus detection, and relates to the technical field of qPCR. The invention provides a qPCR (quantitative polymerase chain reaction) buffer solution. The qPCR buffer solution comprises the following components: SYBR Green I, a hot start Taq enzyme, Tris, KCl, MgCl2, (NH4) 2SO4, dNTPs (deoxyribonucleoside triphosphates), choline chloride and hexadecyl trimethyl ammonium bromide. When the qPCR buffer solution is used for detection, the repeatability is good, the detection limit is low, the anti-interference capability is high, and the application potential in virus detection is huge.

The invention belongs to the technical field of cold chain detection sampling, and particularly discloses virus inspection equipment for logistics, which comprises a shell, the surface of the shell is slidably connected with a baffle, the inner wall of the shell is fixedly connected with a placement plate, the bottom of the placement plate is fixedly connected with a partition plate, and the partition plate is fixedly connected with the baffle. A collecting area is formed between one side of the partition plate and the shell, and a discharging opening communicated with the collecting area is formed in the surface of the shell. Through the synergistic effect of the first electric push rod, the rotary table, the second electric push rod and the driving assembly, automatic sample pressing, frost scraping, rotary sampling and continuous sample layout are achieved. The whole process is high in automation degree, an operator only needs to place and take out a sample, tedious manual wiping operation is avoided, the device is particularly suitable for rapid screening of large-batch cold chain goods, the working efficiency is remarkably improved, frost on the surface of the sample can be automatically scraped off before sampling, and the working efficiency is improved. The problem that the virus is diluted by frost and is difficult to detect in the sampling process of a frost layer interference swab is avoided.

The invention relates to the field of virus detection, in particular to a specific primer probe combination, a method and a kit for identifying avian influenza virus H9 prevalent strains SZ, A and X. The sequences of the three pairs of primers are as shown in SEQ ID NO: 1-9. The primer probe combination provided by the invention can achieve the purpose of distinguishing and detecting different strains of avian influenza H9 through fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction). The primer probe combination provided by the invention has the advantages of high sensitivity, strong specificity and the like, and provides a new effective detection means for rapid differential diagnosis, epidemiological investigation and prevention and control of the avian influenza virus H9 clinically.

The invention belongs to the technical field of virus detection, and particularly relates to a nucleic acid detection primer, a probe, a detection kit, a detection method and application of an immature skin-adding virus. The primer, the probe and the reaction system designed by the invention are high in sensitivity, the lowest detection limit can reach 3 copies / mL, the accuracy is 100%, the specificity is high, and cross amplification with other 76 pathogens is avoided. The kit adopts a recombinase polymerase isothermal amplification technology, does not need a large-scale PCR instrument, can complete detection only in 25 minutes, saves about 2 hours of time compared with a traditional fluorescent PCR detection method, adopts an M13 bacteriophage probe as internal positive quality control, can prevent false negative conditions, and can prevent pollution by adding UNG enzyme. The kit can be used for laboratory detection, field detection and bedside detection, and is especially suitable for detection of the yearly skin-adding virus at quarantine ports, airports and epidemic disease outbreak sites.

The application belongs to the technical field of virus detection, and discloses a digital PCR kit for simultaneously detecting bovine dermatophilosis virus, Schmallenberg virus and peste des petits ruminants virus, which comprises primers and probes shown as SEQ ID NO. 1-SEQ ID NO. 18. After single system tests on bovine dermatophilosis virus-ORF101 gene, bovine dermatophilosis virus-ORF126 gene, Schmallenberg virus, peste des petits ruminants virus-N gene and peste des petits ruminants virus-M gene, a six-repeated digital PCR system containing an internal standard is established. After tests on indexes such as linearity, specificity, minimum detection limit, repeatability, single and multiple digital PCR systems, the developed digital PCR multiple detection kit can fully meet the development requirements, has strong specificity, high sensitivity, and the minimum detection limit can reach below 10 Copies / μL.

The invention provides a fluorescent quantitative PCR (polymerase chain reaction) detection primer, a kit and a method for a novel variable chalmavirus VP gene, and belongs to the technical field of virus detection. A specific primer is designed and synthesized according to a conserved sequence of a VP gene of the novel variant chalmavirus, a recombinant plasmid is constructed, then the recombinant plasmid is used as a standard substance to establish a standard curve, a real-time fluorescent quantitative PCR detection system aiming at the novel variant chalmavirus is constructed, and specific detection of the novel variant chalmavirus is realized. The detection method can be used for quantitatively detecting the novel variant chalmavirus in the to-be-detected sample, and has the characteristics of high speed, high sensitivity, strong specificity and the like; the method provides a key technical support for molecular epidemiological investigation of the Sepamavirus in animal populations, is helpful for realizing early screening of infected individuals and timely blocking of virus transmission, and has important practical significance for guaranteeing population health and maintaining high mountain ecosystem stability.

The application discloses a parrot beak feather disease virus probe fluorescence quantitative detection primer, a kit and application thereof, and belongs to the technical field of virus detection. The detection primer comprises an upstream primer PBFDV-F with a nucleotide sequence as shown in SEQ ID NO. 3, a downstream primer PBFDV-R with a nucleotide sequence as shown in SEQ ID NO. 4 and a probe PBFDV-P with a nucleotide sequence as shown in SEQ ID NO. 5. The application designs parrot beak feather disease virus specific detection primers and Taq Man probes, constructs a recombinant standard plasmid, optimizes a reaction system and establishes a parrot beak feather disease virus detection method. The sensitivity, specificity and repeatability of the detection method are verified through experiments, and it is found that the detection method has good sensitivity, specificity and repeatability. The application provides reliable technical support for clinical diagnosis and epidemiological investigation of PBFD.

The application discloses a virus searching and killing method and system for distributed energy safety data, relates to the field of virus processing, and comprises the following steps: acquiring a virus original characteristic code, splitting the original characteristic code into N discontinuous but sequentially fixed segments F i , calculating the starting offset S i of each segment F i relative to the first segment F1, and constructing a virus characteristic database; then, the data stream is divided into multiple data blocks B i of fixed size, a fixed-size sliding window is adopted to perform scanning and matching association, and finally, threshold judgment is performed; the original virus characteristic code is subjected to fragmentation processing, the storage requirement of the virus characteristic database is reduced; the sliding window scanning can ensure that any characteristic combination is covered, and a matching event FP i is recorded instead of full data, so that the memory occupation is reduced; through comparison of the absolute offset SB i with an expected position, the correct sequence of the segment in the virus is ensured, a state machine can track the matching progress in real time, and the weight of only the segment with correct sequence is accumulated, so that the detection reliability is improved.