The invention relates to a
microfluidic chip and a manufacturing method thereof. The
microfluidic chip comprises an upper
chip unit and a lower
chip unit; the surfaces of the
chip units are respectively provided with a liquid storing hole, a liquid channel communicated with the liquid storing holes and a microchannel communicated with the liquid channel; the upper chip unit and the lower chip unit are attached to each other and the microchannel is sealed between the two chip units; the
microfluidic chip also comprises a
micro valve, wherein the micro-valve main body is one of the following structures: a three-dimensional
micrometer and three-dimensional nanometer hierarchical structure of polymers formed on the microchannel in array arrangement, a three-dimensional
micrometer structure ofpolymers formed on the microchannel in array arrangement or a three-dimensional nanometer structure of polymers formed on the microchannel in array arrangement; the polymers can generate specific responses to an
external field under the action of the
external field; and the specific responses are reversibility transformation of one or more of
surface chemical compositions of the polymers, the above structures and surface properties, thus realizing controllable operations for the flow state of a microfluid in the microchannel. The
micro valve in the microfluidic chip is manufactured in situ and miniaturized on the microfluidic chip, thus realizing switch gradient control for the flow of the microfluid and fast switch reversibility transformation.