7 results about "Pcr chip" patented technology

A random access automated molecular testing system and method is used with a planar polymerase chain reaction (PCR) chip to provide molecular detection covering a wide variety of assays / tests in a small footprint. An automated transport mechanism moves the PCR chip between a pipette loading station, a sealing station and an amplification and detection module to provide batchless and random-access amplification and detection of a biological sample fluid. The PCR chip a planar rectangular body, a U-shaped channel for receiving sample fluid from an inlet port and a gripping feature laterally extending from an upper surface of the body above the inlet port for use by the autmoated transport mechanism. An amplification and detection module includes a heating block, a clip with a viewing window for retaining the PCR chip and a detection platform for identifying a content characteristic of interest of the sample fluid.

A droplet generation system and detection method. The droplet generation system for digital PCR detection includes a PCR chip having a containing cavity; a microchannel having a first opening and a second opening for liquid to enter and exit; a rotation driving mechanism for driving the microchannel to reciprocating swing; and a fluid driving mechanism for driving the liquid to pass through the microchannel. An end of the microchannel where the first opening is located is capable of being inserted into the containing cavity and reciprocating swing in the containing cavity under the drive of the rotation driving mechanism.

This invention provides a droplet-based digital PCR primer-probe set for detecting *Toxoplasma gondii* and its applications, belonging to the field of molecular biology. This invention targets the conserved region of the *Toxoplasma gondii* SAG1 gene, designing and screening a specific primer-probe set. The forward primer sequence is shown in SEQ ID NO:1, the reverse primer sequence in SEQ ID NO:2, and the probe sequence in SEQ ID NO:3. This invention constructs a reaction system with the nucleic acid of the sample to be tested, the primer-probe set, and a PCR premix. After dropletization or loading onto a digital PCR chip, amplification is performed, and absolute quantification is achieved through positive droplet counting and Poisson distribution. The detection method of this invention has high sensitivity, strong specificity, and good repeatability. This invention provides an efficient and reliable technical means for the early diagnosis, detection of low-load samples, and quantitative monitoring of *Toxoplasma gondii* infection.

The application discloses a PCR chip for monitoring Nipah virus and products and applications thereof, and relates to the technical field of biological detection. The application provides a PCR chip for monitoring Nipah virus, which comprises the following primers and probes: primers and probes for detecting Nipah virus M subtype; and primers and probes for detecting Nipah virus B subtype. The kit has excellent detection capability for Nipah virus M subtype, Nipah virus B subtype and Hendra virus, is high in sensitivity, good in specificity, accurate and reliable in detection result, strong in repeatability, simple in operation and short in time consumption. The kit can simultaneously realize the differentiation of Nipah virus M / B subtypes and the identification of Hendra virus in a single sample, and provides technical support for the rapid identification of Nipah virus and Hendra virus.

This invention discloses an integrated digital PCR system and its usage method. The system comprises a high-density microcavity array digital PCR chip with an integrated cavity, an optical detection module, a hot water circulation temperature control module, a fluid control module, and a central control module. When applied to nucleic acid sample detection, the digital PCR chip is first fixed on the stage of the optical detection module, and connecting tubes for the sample tubes and hot water tank are installed at each chip interface. Then, the central control module is activated, and relevant parameters of the control program are set. The control program then controls the fluid control module, hot water circulation temperature control module, and optical detection module to sequentially perform operations such as sample filling, sample discretization, hot water circulation amplification, chip scanning and recording, and data analysis. Based on the combination of the microcavity array and integrated cavity structure of the digital PCR chip, this system allows the entire digital PCR detection process to be completed on a single instrument, improving detection efficiency and result reliability.

The application relates to the fields of molecular biology and environmental biotechnology, and discloses a PCR chip, a kit and application thereof. The PCR chip comprises a chip plate, primer pairs for specifically amplifying bvcA genes, cfrA genes, mbrA genes, pceA genes, pteA genes, tceA genes, vcrA genes and internal reference genes are loaded on the chip plate, and a gradient dilution template of an internal reference gene for drawing a standard curve is arranged on the chip plate; the gradient dilution template of the internal reference gene is obtained by gradient dilution of internal reference gene template DNA and addition of internal reference gene primers. The PCR chip can detect absolute quantification of chlorinated hydrocarbon microbial degradation related reduction dehalogen functional genes through qPCR technology, so that the PCR chip can be used for accurately detecting the copy number of the chlorinated hydrocarbon microbial degradation related reduction dehalogen functional genes, thereby the feasibility of chlorinated hydrocarbon pollution bioremediation can be explored, the chlorinated hydrocarbon pollution bioremediation process can be evaluated, and the chlorinated hydrocarbon pollution bioremediation method can be further assisted and guided.

This utility model discloses a real-time fluorescence ultrafast photothermal PCR amplification and detection device, belonging to the field of intelligent medical testing instrument technology. It includes a laser probe and an adjustable laser power supply. An infrared temperature sensor is coaxially fixed to one side of the laser probe. A chip chamber is located below the laser probe, and a PCR chip is installed within the chip chamber. The PCR chip contains photothermal nanomaterials. A fluorescence feedback component is located below the chip chamber, and an imaging component is connected to the end of the fluorescence feedback component furthest from the chip chamber. This invention solves the technical problems of existing PCR amplification and detection devices, such as long amplification times, inability to observe temperature in real time, and inability to ensure the temperature conditions for thermal cycling amplification of nucleic acid molecules.