22 results about "Pcr chip" patented technology

The invention relates to the technical field of molecular biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of fluoride microbiological degradation related functional genes and application of the PCR chip and the kit. The PCR chip comprises a primer pair which is used for specifically amplifying a DeHa1 gene, a DehH2 gene, an Hddph gene and an alkB gene respectively, and a primer pair which is used for specifically amplifying the DeHa1 gene, the DehH2 gene, the Hddph gene and the alkB gene respectively, the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DeHa1 gene are as shown in SEQ ID NO.1-2; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the DehH2 gene are as shown in SEQ ID NO.3-4; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the Hddph gene are as shown in SEQ ID NO.5-6; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the alkB gene are shown in SEQ ID NO.7-8. The PCR chip can be used for accurately detecting the expression condition of fluoride microbiological degradation related functional genes through a qPCR technology, exploring the fluoride pollution bioremediation feasibility and evaluating the fluoride pollution bioremediation process.

The invention relates to the technical field of molecular biology and environmental biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of functional genes related to microbial degradation of chlorinated hydrocarbon and application of the PCR chip and the kit. The PCR chip comprises a primer pair and a primer pair, the primer pair is used for specifically amplifying dehalogenation functional genes bvcA, cfrA, mbrA, pceA, pteA, tceA and vcrA respectively, the primer pair is used for specifically amplifying reference genes, and nucleotide sequences of the primer pair for specifically amplifying the dehalogenation functional genes are shown as SEQ ID NO. 1 to SEQ ID NO. 14. The PCR chip provided by the invention can specifically amplify seven functional genes related to the degradation of the chlorinated hydrocarbon microorganisms, is good in specificity, high in accuracy and good in repeatability, and can accurately detect the expression condition of the seven dehalogenation functional genes related to the degradation of the chlorinated hydrocarbon microorganisms through a qPCR technology, so that the existence condition of chlorinated hydrocarbon degrading bacteria can be reflected; the method has reliability in the aspects of chlorohydrocarbon polluted site biodegradability evaluation, degradation process research, repair effect verification and the like.

The invention provides a PCR chip, a manufacturing method thereof and a PCR chip detection method. The PCR chip comprises a substrate layer and a plurality of reaction units which are arranged on the substrate layer in an array mode, and each reaction unit comprises a reaction chamber and a plurality of silicon-based nano columns which are arranged in the reaction chamber in an array mode. In the invention, by utilizing the characteristic that the nano-scale metasurface array based on the silicon-based material can enhance the fluorescence signal, the plurality of silicon-based nano columns arranged in the array are added in the reaction unit and are used for enhancing the PCR reaction fluorescence signal and improving the resolution ratio of the fluorescence intensity, so that the purposes of improving the detection sensitivity of the PCR chip and reducing the detection error are achieved.

An apparatus, multi-well plate and method for automated cell lysis and nucleic acid purification and amplification. The plate includes a lysis well, at least one wash well, an elution well, and a PCR chip. The apparatus includes a vertically aligned rotor mixer comprising a magnetic tip and actuators for moving the rotor mixer in a vertical and horizontal directions, to transfer magnetic beads from well to well. The rotor mixer is used to vortex lysis mixtures, wherein the vortexing speed is sufficient to overcome the magnetic attraction between the beads and mixer tip and disperse the beads in solution, to collect nucleic acids such as DNA in an elution solution that is transferred to the PCR chip for amplification of target sequences.

The invention relates to a microfluidic real-time quantitative PCR chip system, which comprises a support and a plurality of chips, the adjacent chips are spliced with each other and detachably installed and fixed on the support, and each chip is formed by compounding a bottom sealing cover layer, a core function layer and a top sealing cover layer, a lower-layer micro-channel network and an upper-layer micro-channel network are constructed on the two opposite sides of the core function layer respectively, and the lower-layer micro-channel network and the upper-layer micro-channel network are subjected to airtight packaging through the bottom-layer sealing cover layer and the top-layer sealing cover layer respectively, so that a complete inner cavity fluid control system is formed. According to the microfluidic real-time quantitative PCR chip system disclosed by the invention, the problems of high reagent consumption and microfluidic chip flux solidification in a traditional scheme are solved at the same time through an extensible architecture design, so that not only can the usage amount of reagents be reduced, but also the flux and flexibility of an experiment can be improved, and a user is allowed to quickly adjust the detection flux according to specific experiment requirements; therefore, optimal configuration of resources and maximization of experiment efficiency are realized.

A random access automated molecular testing system and method is used with a planar polymerase chain reaction (PCR) chip to provide molecular detection covering a wide variety of assays / tests in a small footprint. An automated transport mechanism moves the PCR chip between a pipette loading station, a sealing station and an amplification and detection module to provide batchless and random-access amplification and detection of a biological sample fluid. The PCR chip a planar rectangular body, a U-shaped channel for receiving sample fluid from an inlet port and a gripping feature laterally extending from an upper surface of the body above the inlet port for use by the autmoated transport mechanism. An amplification and detection module includes a heating block, a clip with a viewing window for retaining the PCR chip and a detection platform for identifying a content characteristic of interest of the sample fluid.

A droplet generation system and detection method. The droplet generation system for digital PCR detection includes a PCR chip having a containing cavity; a microchannel having a first opening and a second opening for liquid to enter and exit; a rotation driving mechanism for driving the microchannel to reciprocating swing; and a fluid driving mechanism for driving the liquid to pass through the microchannel. An end of the microchannel where the first opening is located is capable of being inserted into the containing cavity and reciprocating swing in the containing cavity under the drive of the rotation driving mechanism.

The invention discloses a high-flux real-time fluorescent quantitative PCR (polymerase chain reaction) chip for detecting soil-transmitted pathogenic bacteria and a detection method of the high-flux real-time fluorescent quantitative PCR chip. The chip comprises a primer composition consisting of 64 pairs of primer pairs which are designed for specific genes of 47 species and 7 genera of soil-propagated plant pathogenic fungi, 8 species of soil-propagated plant pathogenic bacteria and two reference genes. On the basis of a high-flux quantitative PCR (HT-qPCR) technology, a novel chip capable of simultaneously detecting 47 species and 7 genera of soil-transmitted plant pathogenic fungi and 8 species of soil-transmitted plant pathogenic bacteria is established, and a powerful tool is provided for evaluating potential risks of soil-transmitted plant pathogens in an environment. The method is applied to pathogenic bacteria spread in soil, the abundance and distribution of the pathogenic bacteria spread in the soil can be detected and analyzed, the defects of low-flux amplification of traditional single-gene qPCR and high cost of metagenomics are overcome, and the method has wide application prospects.

This invention provides a droplet-based digital PCR primer-probe set for detecting *Toxoplasma gondii* and its applications, belonging to the field of molecular biology. This invention targets the conserved region of the *Toxoplasma gondii* SAG1 gene, designing and screening a specific primer-probe set. The forward primer sequence is shown in SEQ ID NO:1, the reverse primer sequence in SEQ ID NO:2, and the probe sequence in SEQ ID NO:3. This invention constructs a reaction system with the nucleic acid of the sample to be tested, the primer-probe set, and a PCR premix. After dropletization or loading onto a digital PCR chip, amplification is performed, and absolute quantification is achieved through positive droplet counting and Poisson distribution. The detection method of this invention has high sensitivity, strong specificity, and good repeatability. This invention provides an efficient and reliable technical means for the early diagnosis, detection of low-load samples, and quantitative monitoring of *Toxoplasma gondii* infection.

The application discloses a PCR chip for monitoring Nipah virus and products and applications thereof, and relates to the technical field of biological detection. The application provides a PCR chip for monitoring Nipah virus, which comprises the following primers and probes: primers and probes for detecting Nipah virus M subtype; and primers and probes for detecting Nipah virus B subtype. The kit has excellent detection capability for Nipah virus M subtype, Nipah virus B subtype and Hendra virus, is high in sensitivity, good in specificity, accurate and reliable in detection result, strong in repeatability, simple in operation and short in time consumption. The kit can simultaneously realize the differentiation of Nipah virus M / B subtypes and the identification of Hendra virus in a single sample, and provides technical support for the rapid identification of Nipah virus and Hendra virus.

The invention discloses a tray assembly, a bearing unit, a chip conveying module and a digital PCR instrument. In the tray assembly, under the condition that the first pressing plate, the second pressing plate, the base plate and the locking assemblies are matched to bear and lock the digital PCR chips at the same time, the first pressing plate abuts against the surfaces, close to the first side edges of the digital PCR chips, of the digital PCR chips at the same time; the second pressing plate abuts against the surfaces, close to the second side edges of the digital PCR chips, of the digital PCR chips at the same time, and the first side edge and the second side edge of each digital PCR chip are the two opposite sides of the digital PCR chip. Only the surfaces of the two opposite sides of each digital PCR chip are pressed in an abutting mode, so that each digital PCR chip can release stress in time in the abutting and clamping process in a self-adaptive mode, self-adaptive abutting and clamping are achieved, and then the digital PCR chips can be effectively prevented from deforming.

The utility model discloses an air tightness detection device for a PCR chip for detecting a plurality of micro-channels, and relates to the technical field of PCR chips, the air tightness detection device comprises a bottom plate, a first vertical plate and a second vertical plate, the first vertical plate and the second vertical plate are respectively and fixedly arranged on two sides of the upper surface of the bottom plate, a rotating rod is rotatably arranged on the inner side of the first vertical plate, and the rotating rod is rotatably arranged on the inner side of the second vertical plate. A rotating shell is fixedly arranged at one end of the rotating rod, a motor is fixedly arranged on the outer wall of the first vertical plate, the output end of the motor is fixedly connected with one end of the rotating rod, a fixing pipe is fixedly arranged on the inner side of the second vertical plate, a shielding shell is fixedly arranged on the rod wall of the fixing pipe, and one end of the fixing pipe extends into the shielding shell. And an air pump is fixedly arranged on the outer wall of the second vertical plate. The PCR chip air tightness detection device is provided with a plurality of groups of profiling pressing plates, can be connected with different PCR chips, and can be quickly switched, so that efficient air tightness detection work of different types of PCR chips can be realized.

This invention discloses an integrated digital PCR system and its usage method. The system comprises a high-density microcavity array digital PCR chip with an integrated cavity, an optical detection module, a hot water circulation temperature control module, a fluid control module, and a central control module. When applied to nucleic acid sample detection, the digital PCR chip is first fixed on the stage of the optical detection module, and connecting tubes for the sample tubes and hot water tank are installed at each chip interface. Then, the central control module is activated, and relevant parameters of the control program are set. The control program then controls the fluid control module, hot water circulation temperature control module, and optical detection module to sequentially perform operations such as sample filling, sample discretization, hot water circulation amplification, chip scanning and recording, and data analysis. Based on the combination of the microcavity array and integrated cavity structure of the digital PCR chip, this system allows the entire digital PCR detection process to be completed on a single instrument, improving detection efficiency and result reliability.

The application relates to the fields of molecular biology and environmental biotechnology, and discloses a PCR chip, a kit and application thereof. The PCR chip comprises a chip plate, primer pairs for specifically amplifying bvcA genes, cfrA genes, mbrA genes, pceA genes, pteA genes, tceA genes, vcrA genes and internal reference genes are loaded on the chip plate, and a gradient dilution template of an internal reference gene for drawing a standard curve is arranged on the chip plate; the gradient dilution template of the internal reference gene is obtained by gradient dilution of internal reference gene template DNA and addition of internal reference gene primers. The PCR chip can detect absolute quantification of chlorinated hydrocarbon microbial degradation related reduction dehalogen functional genes through qPCR technology, so that the PCR chip can be used for accurately detecting the copy number of the chlorinated hydrocarbon microbial degradation related reduction dehalogen functional genes, thereby the feasibility of chlorinated hydrocarbon pollution bioremediation can be explored, the chlorinated hydrocarbon pollution bioremediation process can be evaluated, and the chlorinated hydrocarbon pollution bioremediation method can be further assisted and guided.

A system includes a thermocycling assembly configured to perform thermocycling for a digital polymerase chain reaction (PCR). The thermocycling assembly includes a flat thermal plate having a first side configured to contact and to thermally interface with a flat digital PCR cartridge including thousands of chambers for samples. The thermocycling assembly also includes a heat sink disposed beneath the flat thermal plate including an internal liquid conduit. The thermocycling assembly further includes thermal electric cooling elements disposed between the flat thermal plate and the heat sink, wherein the thermal electric cooling elements are configured to regulate a temperature of the flat thermal plate during the thermocycling. The thermocycling assembly even further includes a liquid cooling system coupled to the heat sink and configured to flow a liquid through the internal liquid conduit to facilitate rapid cooling of the flat thermal plate during thermocycling.

The invention discloses a digital PCR chip which comprises a liquid drop detection area and anchoring assemblies, and the anchoring assemblies are arranged at the two opposite ends of the liquid drop detection area at intervals and used for fixing liquid drops in the liquid drop detection area. According to the digital PCR chip provided by the embodiment of the invention, the anchoring assemblies are arranged at the two opposite ends of the liquid drop detection area, so that the liquid drops stably stay in the liquid drop detection area, the liquid drops are kept in the liquid drop detection area after being heated, loss caused by flowing of the liquid drops in the heating process can be effectively reduced, and the detection efficiency of the liquid drops is improved. The digital PCR chip is simple in structure, convenient to operate, beneficial to PCR reaction and fluorescence detection of liquid drops, capable of achieving on-chip liquid drop generation and detection integrated design, capable of avoiding reagent pollution and simplifying operation steps of a user, and therefore the quantitative detection accuracy of the digital PCR chip is improved to a certain degree.

The invention relates to the technical field of molecular biology, and discloses a PCR chip and a kit for detecting the expression quantity of functional genes related to biodegradation of brominated compounds and application of the PCR chip and the kit, and the PCR chip comprises primer pairs for respectively and specifically amplifying genes PbrA1, PbrA2, PbrA3 and RHDA; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the PbrA1 gene are as shown in SEQ ID NO.1-2; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the PbrA2 gene are as shown in SEQ ID NO.3-4; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the PbrA3 gene are as shown in SEQ ID NO.5-6; the nucleotide sequences of an upstream primer and a downstream primer of the primer pair for specifically amplifying the RHDA gene are as shown in SEQ ID NO.7-8. The PCR chip can be used for accurately detecting the expression condition of functional genes related to the reductive dehalogenation effect of the brominated compound, so that the PCR chip can be used for reflecting the pollution and biodegradation conditions of the brominated compound and exploring the bioremediation feasibility of the pollution of the brominated compound.

The utility model discloses a digital PCR (Polymerase Chain Reaction) instrument. The jacking driving device is arranged to drive the heat conduction plate to move, so that the heat conduction plate is close to the digital PCR chip located at the processing station, and after the heat conduction plate is in contact with the lower surface of the digital PCR chip, the heat conduction plate supports the lower surface of the digital PCR chip in the vertical direction under further driving of the jacking driving device, so that the digital PCR chip can be processed, and the digital PCR chip can be processed. And the supported digital PCR chip and the supporting plate relatively move in the direction, so that the supported digital PCR chip can be tightly attached to the heat conduction surface of the corresponding heat conduction plate, and the heat conduction plate can more stably and efficiently conduct heat to the digital PCR chip. Therefore, the digital PCR chip can be efficiently and quickly heated and cooled, and liquid drops contained in the digital PCR chip can be efficiently subjected to PCR amplification, so that the detection efficiency is improved.

A random access automated molecular testing system and method is used with a planar polymerase chain reaction (PCR) chip to provide molecular detection covering a wide variety of assays / tests in a small footprint. An automated transport mechanism moves the PCR chip between a pipette loading station, a sealing station and an amplification and detection module to provide batchless and random-access amplification and detection of a biological sample fluid. The PCR chip a planar rectangular body, a U-shaped channel for receiving sample fluid from an inlet port and a gripping feature laterally extending from an upper surface of the body above the inlet port for use by the automated transport mechanism. An amplification and detection module includes a heating block, a clip with a viewing window for retaining the PCR chip and a detection platform for identifying a content characteristic of interest of the sample fluid.

This utility model discloses a real-time fluorescence ultrafast photothermal PCR amplification and detection device, belonging to the field of intelligent medical testing instrument technology. It includes a laser probe and an adjustable laser power supply. An infrared temperature sensor is coaxially fixed to one side of the laser probe. A chip chamber is located below the laser probe, and a PCR chip is installed within the chip chamber. The PCR chip contains photothermal nanomaterials. A fluorescence feedback component is located below the chip chamber, and an imaging component is connected to the end of the fluorescence feedback component furthest from the chip chamber. This invention solves the technical problems of existing PCR amplification and detection devices, such as long amplification times, inability to observe temperature in real time, and inability to ensure the temperature conditions for thermal cycling amplification of nucleic acid molecules.

The application discloses a droplet PCR chip and a droplet PCR detection system. The droplet PCR chip comprises at least one unit, and the unit comprises: an oil phase reservoir and a PCR reagent reservoir, the PCR reagent reservoir is connected with the oil phase reservoir through a fluid flow path, and the two reservoirs are combined and flow to a droplet generation channel; a droplet spreading cavity, one end of the droplet spreading cavity is communicated with the droplet generation channel to receive the generated droplet, and the droplet spreading cavity provides a place for the droplet to perform a PCR amplification reaction; and an outlet reservoir connected to the other end of the droplet spreading cavity to discharge the air in the droplet spreading cavity which is pressed by the droplet; wherein each reservoir is also used to receive mineral oil to seal the droplet spreading cavity. Thus, the air bubbles generated in the oil phase by heating are prevented from leaking out and expelling the droplets out of the droplet spreading cavity, and since the sealing, the vapor pressure generated in the oil phase by heating is retained inside the droplet PCR chip, the vapor pressure inside the droplet spreading cavity is improved, and thus the air bubbles generated in the droplet PCR chip can be reduced or avoided.

This invention discloses a micro-space rapid temperature-changing PCR system, comprising: a PCR chip with a PCR reaction chamber internally connected to a flow channel, a shape memory metal spring and a rubber stopper connected to the shape memory metal spring within the flow channel; and a cooling module including a phase change tank, a flow channel, and a working fluid collection box, wherein the phase change tank is located at the bottom of the PCR chip and is connected to the working fluid collection box via the flow channel. By sealing the reaction chamber with a shape memory metal spring within the flow channel, evaporation of the liquid during high-temperature reactions is prevented; infrared heating technology is employed, allowing the heat source to be directly absorbed by the reaction solution, eliminating thermal resistance between the heat source and the reaction solution; and jet phase change cooling technology is used to cool the PCR reaction solution, achieving rapid cooling.