Chip structure for PCR (polymerase chain reaction) rapid reaction

A chip structure, rapid response technology, applied in specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, biochemical instruments, etc., can solve the problems of rising processing cost, pollution, waste transfer of reagents, etc. Improved efficiency, high heating/cooling rate, cost saving effect

Active Publication Date: 2013-03-27
ANHUI ANKE BIOTECHNOLOGY (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The static microchamber PCR chip significantly reduces the consumption of the mixed solution, but the temperature control system and structural design directly determine the heating and cooling speed, which still plays a decisive role in the temperature cycle and stability. There is still a gap in speed
Generally, heating ...

Method used

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  • Chip structure for PCR (polymerase chain reaction) rapid reaction
  • Chip structure for PCR (polymerase chain reaction) rapid reaction
  • Chip structure for PCR (polymerase chain reaction) rapid reaction

Examples

Experimental program
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Effect test

Embodiment 1

[0031]PCR chip: Quartz glass is used as the substrate, the size is 80mm×20mm, and the thickness is 2mm. Using laser drilling technology, two rows of microcavity holes are processed on the quartz glass sheet. Each row has eight microcavity holes of the same aperture. Among the rows of microcavity holes, the diameter of one row of microcavity holes is 1.6mm, the diameter of the other row of microcavity holes is 3.0mm, and the depth is 1.5mm. The distance between the centers of the microcavity holes is 9mm. The distance between the hole center and the long side is 8.5 mm, and the distance between the microcavity hole center and the short side is 5.5 mm. The processed quartz glass chip needs to be cleaned by ultrasonic waves. The process is as follows: wash in acetone solution for 5 minutes; take it out and put it in ethanol solution for 5 minutes, put it in deionized water after taking it out, wash it for 5 minutes, and blow dry with nitrogen.

[0032] Cover sheet: PC board mater...

Embodiment 2

[0041] PCR chip: 7740 glass and silicon wafer are used as chip substrates. The 7740 glass has a diameter of 100mm and a thickness of 1.5mm. Two units of 80mm×20mm are drawn on the glass. Using the laser drilling process, each unit has two rows of microcavity holes as through holes, and each row contains eight microcavity holes with the same aperture. The diameters of the two rows of microcavity holes in the same unit are 1.6mm and 3.0mm, respectively. The distance between the centers of the microcavity holes is 9 mm, the distance between the center of the microcavity holes and the long side of the cell is 8.5 mm, and the distance between the center of the through hole and the short side of the cell is 5.5 mm. The silicon wafer has a diameter of 100 mm and a thickness of 500 μm. Using anodic bonding technology, the cleaned glass sheet and silicon sheet with through holes are bonded together to form a PCR chip. The processed glass and silicon wafers need to be cleaned by ultras...

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Abstract

The invention relates to a chip structure for PCR (polymerase chain reaction) rapid reaction. The chip structure for PCR rapid reaction comprises a cover plate, a medium layer, a PCR chip and an external heating structure; through holes are machined on the cover plate; micro cavity holes are arranged on the PCR chip; the medium layer is covered on the PCR chip, and absorbs the cover plate in a negative pressure mode through the through holes on the cover plate; the lower bottom surface of the PCR chip are in tight contact with the external heating structure; the PCR chip can be a single-layer structure, and the micro cavity holes are directly machined; or the PCR chip can be a multi-layer structure, and micro cavity holes are machined on at least one layer, and are then combined with the other base material. PCR amplification is easy to achieve rapidly, and the biochemical reaction speed is improved obviously; the heat capacity of a micro environment of a chip is small, and temperature rising and reduction can be controlled at high speed, and the regional temperature can be detected and controlled accurately; the temperature cycle system volume is reduced, the heat capacity is reduced, and the reaction time is shortened greatly; the reaction liquid volume is reduced, the amplification specificity is intensified, and not only is cost saved, but also the efficiency is improved.

Description

technical field [0001] The invention relates to a chip structure for rapid reaction of PCR, which belongs to the technical field of PCR. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments, which can be regarded as special DNA replication in vitro. The principle of PCR technology is similar to the natural replication process of DNA, and its specificity depends on oligonucleotide primers complementary to both ends of the target sequence. PCR consists of three basic reaction steps: denaturation-annealing-extension: ① Denaturation of template DNA: After the template DNA is heated to about 94°C for a certain period of time, the double-stranded DNA of the template or the double-stranded DNA formed by PCR amplification is dissociated, Make it a single strand so that it can be combined with the primer to prepare for the next round of reaction; ②Annealing (annealing) of the template DNA and the primer...

Claims

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Application Information

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IPC IPC(8): C12M1/00
Inventor 何越周如华张健荣吉赞杨楠
Owner ANHUI ANKE BIOTECHNOLOGY (GRP) CO LTD
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