22522results about "Biochemistry cleaning apparatus" patented technology

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

An automatic material supplementing method based on a real-time dynamic measurement result, the method comprising: collecting a real-time dynamic measurement result, and calculating a change rate of each component within a unit time, wherein the change rate is a consumption rate or a generation rate; selecting a component having the highest change rate as a marker within a current time period; collecting historical measurement data, and calculating a cell growth rate in a logarithmic growth phase and a target product expression rate in a cultivation and harvesting stage; taking, as a set component concentration value within the current time period of the logarithmic growth phase, a concentration level of the marker when the cell growth rate in the logarithmic growth phase is the highest; taking, as a set component concentration value within the current time period of the cultivation and harvesting stage, a concentration level of the marker when the target product expression rate in the cultivation and harvesting stage is the highest; and calculating an addition dosage of the marker within the current time period on the basis of the change rate of the marker and / or the difference between a real-time measured concentration of the marker within the current time period and the set component concentration value within the current time period, and calculating an addition dosage of a culture medium on the basis of the addition dosage of the marker, so as to obtain a material supplementing strategy.

The invention discloses a microbial fermentation strain breeding control system based on a neural network model, and relates to the technical field of digital fermentation, and the system comprises a thallus data acquisition module, a biological feature recognition module, a prediction classification module, a breeding decision module, an optimization training module, an early warning monitoring module and a sorting regulation module. The bacterial data acquisition module preprocesses bacterial data; the biological feature recognition module constructs a bacterial feature library; the prediction classification module predicts the adaptability of bacterial strains and classifies the bacterial strains; the selection decision module selects the bacterial strains and generates culture parameters; the sorting regulation and control module generates a three-dimensional scatter diagram and a hereditary character report. According to the method, the precision and efficiency of breeding of the microbial fermentation strain are remarkably improved, intelligence and automation of the breeding process are achieved, the labor cost is reduced, the stability and controllability of the fermentation process are enhanced, and powerful technical support is provided for innovative development of the microbial fermentation industry.

The present disclosure relates to systems, devices, and methods for electroporating. In an embodiment, the present disclosure relates to an electroporation system, comprising at least one electroporation chamber, each electroporation chamber comprising a first electrode and a second electrode, a plurality of ports comprising an inlet port at a first portion of each electroporation chamber, and an outlet port at a second portion of each electroporation chamber, a fluid conduit arranged between the first electrode and the second electrode, the fluid conduit being in fluid communication with the inlet port and the outlet port, and an automated system for electroporation operatively connected to the first electrode and the second electrode of the at least one electroporation chamber.

Described herein are systems and methods for automated cell transduction within a cell processing system. A system for cell processing may include a cell processing cartridge having a transduction system. The transduction system may include a fluidic manifold, one or more modules for performing a cell processing protocol, and a tube having a surface area to volume ration of between about 1,260 mm2 / mL and about 5,080 mm2 / mL. A method for cell processing may first include flowing cells through a tube of a flow cell of a cell processing cartridge for a first time period to achieve a transduction efficiency of at least 50%. Second, the method may include expanding the cells within a bioreactor module of the cell processing cartridge for a second time period.

The invention discloses a hot air type qPCR instrument, a multi-channel detection optical system and a detection method, and relates to the field of molecular biological detection. The problems that an optical detection system adopted by an existing qPCR instrument mostly depends on a single-channel or double-channel design, sample hole sites need to be switched one by one and detected in a time-sharing mode in the detection process, namely, the efficiency is low in the face of a high-throughput requirement scene are solved, and the system combines multiple LED light sources with an optical filter to generate excitation wavelengths to form a light source module; after the multi-stage dichroscope is utilized to form a light combination module, the light combination module is transmitted to a reaction hole through one branch of the forked optical fiber to excite fluorescent substances, a fluorescent signal is collected through a common end of the forked optical fiber, the other branch of the forked optical fiber outputs a fluorescent signal, and the fluorescent signal is split by the reflection grating to form a light splitting module. And the array CMOS is combined with a spectrum unmixing algorithm to form a detection module for synchronous receiving, so that multi-channel simultaneous high-sensitivity detection is realized. The method is also suitable for the field of multi-pathogen detection and gene expression analysis.

Provided are a double-cavity micro-fluidic chip having a reaction cavity and a test cavity and used for nucleic acid test, and a test method thereof. A fluid channel is provided between the reaction cavity and the test cavity, and only one exhaust channel is provided. The combination of the fluid channel and the exhaust channel can automatically provide resistance for a sample entering the test cavity from the reaction cavity, thereby achieving a controllable transfer of the sample between the reaction cavity and the test cavity. A gravity micro-fluidic chip and a centrifugal micro-fluidic chip are designed. The gravity micro-fluidic chip has a multi-turning circuitous fluid channel of which the diameter is smaller than that of a sample injection channel, such that under the action of gravity, the sample can flow into a reaction cavity from a sample adding region but cannot flow into the fluid channel, and then under pressurization by a simple pressurization device, the sample can enter a test cavity from the reaction cavity. The centrifugal micro-fluidic chip provides an exhaust channel branched from a fluid channel, and makes the exhaust direction opposite to a rotary centrifugal direction and the position of an exhaust opening higher than that of the fluid channel; therefore, by combining the principle of internal and external balance of the atmospheric pressure and the centrifugal driving performance, the centrifugal micro-fluidic chip effectively controls the flowing of a gas and a liquid in reaction cavities and test cavities, so as to effectively control the amount of the sample entering each cavity, so that multiple nucleic acid tests can be completed simply by means of one or two times of centrifugation. In addition, for a chip which only has a reaction cavity, an exhaust channel is connected to a liquid storage cavity, and a gravity-driving chip and an asymmetric pressure-driving chip are designed, thereby effectively avoiding aerosol pollution.

A system and method for quantifying CO2 capture by photobioreactors using microalgal biomass are disclosed. The method involves accurately quantifying carbon dioxide capture by photobioreactors using microalgal biomasses involves determining a unique ratio relating carbon consumed to biomass produced for each microalgae strain. This ratio is then used to predict CO2 removal from flue gases or industrial processes based on the dry mass of the corresponding biomass produced. Machine learning models are also trained on data obtained from the photobioreactors to predict CO2 consumption rates under varying conditions, thereby enhancing the accuracy and efficiency of carbon capture technologies.

Embodiments of the invention include additional methods and compositions for fabricating organoids. A unique approach has been developed for the rapid aggregation of defined number of cells using a micropillar-based template to limit the size of organoids, which are then more commonly referred to as spheroids. This procedure can be utilized to create in vitro cardiac tissue models (e.g., cardiac organoids) simply, rapidly, and inexpensively. In certain aspects an alginate-gelatin based hydrogel premixed with cardiomyocytes is used in combination with a patterned microfluidic chip / cell culture chamber having an array of micropillars to fabricate 3D cardiac organoids.

The invention relates to the technical field of biochemical culture, in particular to a biochemical incubator with multiple bins and a method thereof.The biochemical incubator comprises a case, a cavity is formed in the middle of the case, the cavity is divided into a plurality of culture bins by a plurality of partition plates, treatment cavities are formed in the two sides of the cavity, protective shells are connected to the outer sides of the treatment cavities, and storage assemblies are arranged in the culture bins; the storage assembly comprises a movable bottom plate and three groups of longitudinal partition plates; the longitudinal partition plate divides the movable bottom plate into two storage cavities, storage tables are arranged in the storage cavities, and the movable bottom plate is driven by the pushing assembly to move left and right and used for alternately feeding the two storage tables into the culture bin; two groups of sealing covers are arranged outside the storage table, and are driven to be opened or closed by a turnover assembly; according to the incubator, pollution-free conveying of materials is achieved, and meanwhile the stability of environmental parameters of all the bins is maintained; the culture method ensures that different batches of samples are cultured in an independent environment, and cross contamination between microorganism or cell samples is avoided.

A system for growing cells comprising a bioreactor chamber for growing the cells, a delivery system delivering a perfusion solution to the bioreactor chamber for perfusion of the perfusion solution through the cells, a dialysis system having a dialyzer, a dialysate for performing a dialysis and a filter for reducing ammonia content in said dialysate, and a controller that circulates the perfusion solution through the dialyzer and the dialysate through the filter.

The invention relates to the field of ulcerative colitis, and discloses a multi-parameter monitoring system for evaluating the intestinal barrier function of ulcerative colitis, which is used for realizing the goal of automatically adjusting a treatment scheme according to a monitoring result by constructing a closed-loop feedback treatment system. According to the invention, by designing the nanoprobe of which the surface is modified with a specific binding element and combining with a swallowing capsule endoscopy, real-time imaging and molecular level dynamic tracking of the intestinal barrier are realized, genetically engineered bacteria are constructed to quantify intestinal permeability abnormality and local inflammation level, and an intestinal barrier function damage score and a disease progress prediction result are output; individual monitoring is achieved through transfer learning, ultrasonic elastography, optical coherence tomography, wearable equipment for measuring intestinal electric field impedance spectroscopy and other technologies are adopted, an intestinal wall mechanics-electrophysiology composite parameter set is obtained, and the evaluation accuracy is improved.

There has been demand for an additional method for producing antibodies. The present invention provides: a polymer-coated crosslinked alginate gel fiber in which a core layer containing a crosslinked alginate gel and either antibody-producing cells (e.g., antibody-producing CHO cells) or bioactive-substance-producing cells (e.g., MIN6 cells derived from pancreatic β cells) is coated with a cationic polymer; and a method for producing antibodies, a bioactive substance, etc., using the fiber.

The present disclosure provides systems and methods for processing cells. The systems of the present disclosure may include a compartment, a three-dimensional (3D) matrix, a fluid flow path, an analysis unit, a preservation unit, or any combination thereof. The methods of the present disclosure may process cells via, for example, expansion, culturing, gene delivery, activation, or any combination thereof. The systems and methods described herein may be useful for producing cell products including, for example, cell therapy products.

The invention relates to the technical field of organic fertilizers, and discloses an agricultural waste two-stage fermentation bio-organic fertilizer and a preparation method thereof.The bio-organic fertilizer comprises 70%-80% of a mixture of livestock and poultry manure and straw subjected to anaerobic fermentation and aerobic fermentation two-stage treatment, 5%-10% of auxiliary organic matter, 3%-5% of a soil improvement material, 2%-4% of a functional additive and 1%-3% of a microbial preparation. The preparation method comprises the following steps: mixing the livestock and poultry manure and the straw according to a carbon-nitrogen ratio of 30: 1, and performing pretreatment; carrying out anaerobic fermentation for 7 days, and collecting biogas; performing solid-liquid separation to obtain a solid material and biogas slurry; concentrating the biogas slurry to prepare a leaf fertilizer; adding a microbial preparation into the solid material, and carrying out aerobic fermentation for 10-15 days; after fermentation is completed, drying, functional component adding, granulating, screening and packaging are conducted, a finished product is obtained, collaborative integration of anaerobic-aerobic fermentation and full-quantitative utilization of agricultural waste are achieved, and the method has the remarkable advantages of being short in fermentation period, small in nutrient loss, high in functionality and the like.

The invention provides a micro-fluidic device and a preparation method thereof. The micro-fluidic device comprises a micro-fluidic substrate and a control substrate, the microfluidic substrate comprises a first substrate and a plurality of electrode pairs; the control substrate comprises a second substrate, a control circuit layer and a plurality of contact pairs; the first surface of the first substrate is provided with a plurality of opening areas, each opening area is provided with two accommodating grooves which are arranged at intervals, and the two accommodating grooves are communicated through a microfluidic channel on a common side wall; the plurality of electrode pairs are arranged on the first substrate and are arranged corresponding to the opening area. The control circuit layer is arranged on the second substrate; the plurality of contact pairs are arranged on the second substrate and are electrically connected with the control circuit layer; the control substrate is attached to the microfluidic substrate during use, the plurality of contact pairs are electrically connected with the plurality of electrode pairs, and the control circuit layer is used for applying voltage to each electrode pair. According to the microfluidic device, a plurality of samples are processed in parallel, the detection efficiency is improved, and the replacement cost can be reduced.

A three-dimensional scaffold for use in engineered tissue includes a repeated 3D geometry; and at least one of a hydrogel, a biocompatible polymer, and a bioprintable polymer. The repeated 3D geometry may include an open geometry including a plurality of interconnected nodes configured to be seeded with living cells. The scaffold may be formed via bioprinting. The three-dimensional scaffold may also be used for (1) 3D tissue modelling (for liver, kidney, gut, pancreas, heart, lung, spleen, etc.), (2) disease modeling of different tumor types such as breast, colorectal, lung, liver, brain, ovarian and others, (3) drug testing / therapeutic screening of small molecule drugs, peptides, proteins, antibodies, ADCs, gene therapies, RNA-based therapeutics, cell therapies, and tissue therapies, and (4) therapeutic / drug delivery (i.e., via delivery device that can be used to control drug release based on different patterns).

The utility model discloses a microfluidic system for target object pairing, which comprises a first flow channel, a second flow channel, an oil liquid flow channel, a buffer liquid flow channel, a matching flow channel and a pairing micro valve, a first micro valve, a second micro valve and a buffer liquid micro valve are arranged, the matching flow channel is communicated to the oil liquid flow channel, and the first flow channel, the second flow channel and the buffer liquid flow channel are all communicated to the matching flow channel; when a single first target object and a single second target object exist in the matching runner, the first micro valve and the second micro valve are in a closed state, and the matching micro valve and the buffer solution micro valve are in an open state; and the buffer liquid in the buffer liquid flow channel feeds the single first target object and the single second target object in the flow channel into the oil liquid in the oil liquid flow channel to form liquid drops. According to the utility model, other first target objects and / or other second target objects can be completely prevented from entering liquid drops, so that the success rate of packaging is improved, and the cell loss caused by multiple packaging is reduced.

The invention discloses a maltodextrin waste residue treatment device and method.The maltodextrin waste residue treatment device comprises a fermentation tank, a stirring shaft, a stirring paddle, a first driving mechanism and an air guide bin, the interior of the stirring shaft is hollow to form a first air channel, the interior of the stirring paddle is hollow to form a second air channel, the first air channel is communicated with the second air channel, and a plurality of aeration holes are formed in the stirring paddle; a connecting rod, a spring and a plurality of blocking blocks provided with air passing holes are arranged in the second air channel, the blocking blocks are connected in series through the connecting rod, the spring pulls the connecting rod to block the aeration holes through the blocking blocks, a pull rope is arranged in the first air channel, one end of the pull rope is fixedly connected with the connecting rod, the pull rope pulls the connecting rod to move horizontally, and the air guide bin is installed at the top of the stirring shaft. The air guide bin is rotationally connected with the stirring shaft, an air inlet pipe is arranged on the air guide bin, and an air inlet hole is formed in the stirring shaft. According to the method, the maltodextrin waste residues are subjected to pulse feed fermentation, the ineffective oxygen supply proportion is reduced, the problem of waste of a large amount of energy is solved, the environment is protected, and enterprise benefits are improved.

Provided is a novel cooling tower for xylanase preparation, comprising a water tank platform, a tower body, and support columns, wherein a circular ring block is rotatably connected to the inner wall at the bottom of the water tank platform, an annular cavity is formed along the inner wall of the circular ring block, a plurality of straight rods are evenly distributed in, penetrate through, and are slidably connected to the inner wall of the annular cavity, a scraper is fixedly connected to one end of each straight rod, and the scrapers slidably abut against the inner wall at the bottom of the water tank platform; and a power mechanism for driving the scrapers to clean impurities inside the water tank platform is arranged inside the annular cavity. An annular rack, a plurality of gears, the straight rods, and a scraper structure are provided, the output end of a motor drives rotating shafts fixedly connected thereto to rotate by a certain angle, and by means of the gears, the annular rack, and a toothed belt structure, the rotating shafts drive the straight rods and the scrapers to slide a certain distance, so that the scrapers scrape some impurities remaining on the inner wall at the bottom of the water tank platform, until these impurities are scraped and accumulated in a circular platform hole, and then discharged to the outside for collection.

A reinforcing and scaling construction for a bioprinted tissue model, which comprises a casing and sealing bioink, the casing comprising an inner module comprising a casing base (1) and a perforated cover (2), and an outer module comprising a container (3) comprising technological valves (5), an outer cover (4) and plugs (6) for technological valves (5). The invention also relates to a method for assembling the reinforcing and sealing construction.

The invention belongs to the technical field of inspection and extraction, and particularly relates to an automatic T lymphocyte suction device which comprises an automatic suction device body, the automatic suction device body comprises a bearing table, a rotating disc with a circumferential rotating function is arranged on the bearing table, and a plurality of centrifugal assemblies are evenly distributed on the rotating disc. A centrifugal tube feeding assembly facilitating feeding of centrifugal tubes is arranged in front of one side of the bearing table, a tube carrying and conveying assembly is arranged on the portion, located on one side of the centrifugal tube feeding assembly, of the bearing table, a tube feeding mechanism is arranged at the end of the conveying belt mechanism, and a reaction reagent adding assembly is arranged on one side of the tube carrying and conveying assembly. A sample feeding assembly is arranged on one side of the upper portion of the workbench, and a first suction assembly is arranged above the multi-direction adjusting assembly. According to the invention, multiple samples can be processed in batches, the working efficiency and the cell extraction purity are improved, the influence of manual operation on the result is reduced, the accuracy of the detection effect is ensured, and the use requirements are met.

The invention discloses an integrated fluorescence detection kit and a testing method thereof.The integrated fluorescence detection kit comprises an upper cover assembly, a lysate tube assembly, a main body assembly, a diluent tube assembly and a decorative cover, and the lower end of the upper cover assembly is connected with the lysate tube assembly and the diluent tube assembly; the side wall of the upper cover assembly is connected with the upper part of the main body assembly, and the main body assembly is nested in the decorative cover; a reagent system in the kit comprises an RT-RAA reaction system and a CRISPR reaction system which are stored in the detection kit at normal temperature in the form of freeze-dried microspheres and closed liquid, and a lysis solution and a diluent are released through the lysis solution tube assembly and the diluent tube assembly and react in the main body assembly. According to the invention, the problems that the existing fluorescence detection operation process is complicated, the instrument is not portable and depends on professional analysis and the like are solved, and the rapid, portable and user-friendly detection on the virus is realized based on the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) detection system.

The invention discloses a probiotic fermentation device, and relates to the technical field of fermentation devices, the probiotic fermentation device comprises a fermentation tank, a tank cover is mounted on the fermentation tank, a fixed plate is fixedly connected to the inner wall of the fermentation tank, a gas collection plate is slidably connected to the inner wall of the fermentation tank close to the upper part of the fixed plate, and a ventilation shaft is rotatably connected to the middle part of the tank cover; the middle part of the gas collecting plate is slidably connected to the outer surface of the ventilation shaft, and the outer surface of the middle part of the ventilation shaft is rotatably connected to the fixed plate; compared with the prior art, the device has the advantages that the ventilation shaft rotates under the action of driving force, the ventilation stirring plate can be driven to move together, comprehensive stirring operation is carried out on mixed thalli in the fermentation container, uniform distribution of the thalli is promoted in the stirring process, the contact opportunity of the thalli and oxygen is greatly increased, and the fermentation efficiency is improved. Therefore, the fermentation rate and efficiency of the probiotics are obviously improved.