32 results about "Enzyme electrode" patented technology

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The invention discloses a dynamic blood glucose monitoring and intelligent pump speed adjusting system and method, and relates to the technical field of biomedical engineering.The dynamic blood glucose monitoring and intelligent pump speed adjusting method comprises the steps that precise monitoring is conducted through a multi-mode sensing module, an enzyme electrode sensor adopts an anti-interference coating, a Raman spectrum dynamic correction algorithm is combined, and motion artifacts and temperature drift are restrained; the metabolic feature fusion calibration module is integrated with a time convolution network, time sequence modeling is carried out on diet, exercise and historical blood glucose data, an individualized metabolic parameter database is constructed, and self-adaptive signal drift compensation is achieved; the self-adaptive pump control module innovatively designs a double-layer fuzzy PID controller, a base layer generates an infusion rate according to a real-time blood glucose state and insulin activity, an optimization layer introduces an LSTM metabolism prediction model, and the slope of an infusion curve is dynamically adjusted in combination with future behavior pre-judgment; in the edge-cloud collaborative architecture, a lightweight TCN model is locally deployed to guarantee real-time control, and a cloud end optimizes a global digital twinborn model through federal learning and generates a multi-dimensional metabolic risk early warning report.

The invention provides an enzyme activity double-channel detection device in a high-pressure environment, which comprises a detection shell, a feeding port is downwards formed in the upper end of the detection shell, a reaction cavity is downwards formed in the bottom of the feeding port of the detection shell, the feeding port is detachably connected with a sealing cover, the sealing cover is connected with a sensor insertion shaft extending into the reaction cavity, and the sensor insertion shaft is connected with a sensor. A temperature sensor, a hydraulic sensor and an immobilized enzyme electrode are arranged at the free end of the sensor insertion shaft; a pressure pump is arranged outside the detection shell, and the outlet end of the pressure pump is communicated with the upper part of the reaction cavity through a pressure pipe; observation openings are inwards formed in the two sides of the detection shell, a transparent glass window is arranged on the side wall, at each observation opening, of the reaction cavity, a microscope lens group is arranged in each observation opening, and a spectrophotometer used for covering one observation opening is arranged on one side of the detection shell. According to the invention, enzyme activity determination can be carried out in a high-pressure environment, and the understanding of an adaptation mechanism of life under extreme conditions is greatly promoted.

The invention discloses a carbon material modified epinephrine photo-thermal enzyme electrode, a preparation method and application, and relates to the technical field of biosensing, the preparation method of the enzyme electrode comprises the following steps: S1, mixing citric acid, ethanediamine and formamide for reaction, filtering, purifying and centrifuging to prepare a carbon nano network solution; s2, sequentially adding gold trichloride and a reducing agent into the carbon nano network solution for reaction to prepare a carbon material loaded with gold nanoparticles; s3, dispersing the carbon material in a thiohydracrylic acid aqueous solution, then adding N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide, and carrying out an activation reaction, so as to prepare a functionalized carbon material; and S4, mixing the functionalized carbon material with a laccase solution, heating in a water bath, and dispensing the mixed solution on the surface of the electrode to prepare the epinephrine photo-thermal enzyme electrode. The prepared high-sensitivity photo-thermal laccase electrode has the advantages of simple method, mild conditions, wide detection limit, high sensitivity, good stability and the like.

A biofuel cell generates electricity by an oxidation-reduction reaction catalyzed by an enzyme, wherein the enzyme is immobilized on at least one of an anode and a cathode, the anode comprises a conductive material (1) and an electrode binder (1), the cathode comprises a conductive material (2) and an electrode binder (2), at least one of the electrode binder (1) and the electrode binder (2) comprises a polymer (A), the polymer (A) comprises a first structural unit derived from a nonionic ethylenically unsaturated monomer (a1), a second structural unit derived from an anionic ethylenically unsaturated monomer (a2), and a third structural unit derived from a crosslinking agent (a3), and the crosslinking agent (a3) has two or more ethylenically unsaturated bonds in one molecule.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

To provide a biosensor capable of more accurately measuring the concentration of amino acids in a sample solution.SOLUTION: The present disclosure, in one aspect, relates to a biosensor including an enzyme electrode including a conductive layer containing a conductive material and a reagent layer adjacent to the conductive layer, the reagent layer including an amino acid oxidase and a polymer-type enzyme immobilizer. The polymer-type enzyme immobilizer is preferably a hydrogel.SELECTED DRAWING: Figure 1

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

In one aspect, the present disclosure provides a biosensor capable of providing a highly accurate measurement of the concentration of an amino acid in a solution to be measured. In one aspect, the present disclosure pertains to a biosensor comprising an enzyme electrode that includes a conductive layer containing a conductive material and a reagent layer adjacent to the conductive layer. The reagent layer contains an amino acid oxidase and a polymeric enzyme immobilization agent. The polymeric enzyme immobilization agent is preferably a hydrogel.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The present disclosure provides, among other features, an enzyme electrode in which an enzyme is immobilized via more appropriate bonds. An enzyme electrode according to the present disclosure includes an electrode and a modified protein having an amino acid sequence obtained by substituting at least one lysine residue in a protein that is a reductase or a dehydrogenase with a non-lysine amino acid residue, in which the modified protein is immobilized on a surface of the electrode.

A biosensor comprising an enzyme electrode that contains a conductive layer and a reagent layer adjacent to the conductive layer, wherein the conducive layer contains a mediator, and the reagent layer contains an amino acid oxidase and a cured product of a photosensitive polymer.

The invention relates to the field of electrochemical biosensors, in particular to a crosslinking process method of an enzyme membrane in a biosensor and a preparation method of an enzyme electrode. A cross-linking process method of an enzyme membrane in a biosensor comprises the following steps: spraying a cross-linking solution to the surface of the enzyme membrane, so that the fogdrop-shaped cross-linking solution permeates from the surface of the enzyme membrane to the inside of the enzyme membrane, and realizing different cross-linking densities of the inside and the outside of the enzyme membrane under the support of a polymer during incubation to obtain an enzyme electrode. According to the cross-linking process method of the enzyme membrane, the cross-linking reagent is atomized and evenly arranged on the enzyme membrane in a spraying mode, the prepared enzyme electrode is high in sensitivity and good in stability, meanwhile, operation is easy, batch production is easy, and the production cost is low.

The present invention belongs to the field of immobilized enzyme technology, and specifically relates to an immobilized enzyme electrode and its preparation method and application. The immobilized enzyme electrode provided by the present invention comprises a conductive carrier and a ferredoxin-NADP+ reductase and a reaction enzyme loaded on the conductive carrier; the conductive carrier comprises a conductive substrate and a porous graphene layer arranged on the surface of the conductive substrate, and the pore structure of the porous graphene layer is nanopores; the ferredoxin-NADP+ reductase and the reaction enzyme are loaded in the pore structure of the porous graphene layer. The immobilized enzyme electrode provided by the present invention can realize NADPH cyclic regeneration under the condition of an external power supply, ensure that the enzyme cascade reaction is continuous and stable; and the immobilized enzyme electrode can be used repeatedly, effectively reducing the cost of the enzyme cascade reaction, and is suitable for industrial expansion and application.

The application belongs to the technical field of electrochemical detection and analysis of stress biomarkers, and relates to an MBAzy heterojunction nanoscale enzyme, an electrode system, a flexible sensor and a detection method. 4 / 3 The MBAzy heterojunction nanoscale enzyme is a composite material of a single-atom nanoscale enzyme CuPC and MBene, the MBene is Mo B2MBene, and the mass ratio of the CuPC to the MBene is 10-20:1.The MBAzy heterojunction nanoscale enzyme can catalyze dopamine to generate electrons and cause an electrochemical reaction. The method based on the MBAzy heterojunction nanoscale enzyme can sensitively detect dopamine in a life flowing body.

The intelligent blood glucose monitoring device comprises a shell, a protection plate is hinged to the upper end of the shell, an enzyme electrode sensor is arranged in the middle of the bottom wall of the shell, fixing bandages are rotationally connected to the middles of the left end and the right end of the shell, and the intelligent blood glucose monitoring device further comprises a connecting mechanism and a fixing mechanism; the connecting mechanism comprises a socket, an inserting rod, a mounting seat, a connecting seat and driving plates, the socket is arranged on the lower side of the right end of the fixing bandage on the left side, the inserting rod is arranged on the right side in the socket, the connecting seat is slidably connected into a sliding groove formed in the middle of the bottom wall of the socket, and the driving plates are rotatably connected to the upper sides of the front end and the rear end of the connecting seat; according to the intelligent blood glucose monitoring device for gestational diabetes, the problem that the fixing bandage is loosened due to the fact that the magnet is pulled open accidentally is solved, the fixing effect is further improved, the operation process is simplified, and the use convenience is further improved.

The present invention relates to an enzyme electrode for glucose measurement. The enzyme electrode comprises an electrode substrate layer and a sensing layer covering the electrode substrate layer, wherein the sensing layer comprises: an NAD(P)(H)-dependent glucose oxidoreductase and an NAD(P)(H) oxidoreductase. The enzyme electrode further comprises a first polymer layer covering the sensing layer, the first polymer layer being used for protecting the sensing layer and / or restricting the permeation of glucose and / or an interferant. The enzyme electrode further comprises NAD(P)(H) which is located on the sensing layer; and / or an NAD(P)(H) derivative which is a polymer covalently bound to NAD(P)(H) and located on the sensing layer and / or the first polymer layer. The enzyme electrode of the present invention can achieve stable, accurate and / or anti-interference continuous measurement of the blood glucose level.

The invention relates to the technical field of medical instruments, in particular to instant blood glucose automatic detection equipment based on an enzyme electrode biosensor, which comprises a blood glucose meter main body, a sample frame is mounted in the blood glucose meter main body, a lifting mechanism is mounted in the blood glucose meter main body, and a sampling needle is mounted on the lifting mechanism. The detection equipment disclosed by the invention is based on the enzyme electrode biosensor, the detection method is accurate and reliable, the measurement range is 0.5-50mmo l / L (9-900mg / dL) of glucose, the repeatability and the accuracy are high, clinical detection requirements can be met, the equipment realizes an automatic detection process, the operation is simple and convenient, manual operation errors are reduced, the detection efficiency is improved, the equipment is suitable for being used by medical institutions and families, and the method is worthy of popularization and application. A perfect safety protection mechanism and a maintenance system are provided, safe operation and long-term stability of equipment are guaranteed, the use risk is reduced, an operation interface is friendly, functions are rich, and a user can conveniently carry out detection operation, data management and equipment setting.

The present invention discloses a blood glucose and uric acid electrochemical sensor and a preparation and detection method. The electrochemical sensor includes a lower substrate layer, a printed electrode layer, a reagent layer and an insulating layer arranged in sequence from bottom to top, and the printed electrode layer includes a first electrode group consisting of a first working electrode and a first reference electrode, a second electrode group consisting of a second working electrode and a second reference electrode, and each electrode is connected to a corresponding wire; the reagent layer and the first electrode group form an enzyme electrode for measuring the blood glucose concentration of a blood sample, and the second electrode group is used for measuring the hematocrit and uric acid concentration of the blood sample, and the second working electrode is subjected to surface hydrophilization treatment. According to the signal measured by the electrochemical sensor, the blood glucose and uric acid concentrations can be obtained. The present invention can completely avoid the mutual interference of the two enzymes of blood glucose detection and uric acid detection, ensure the accuracy of the test results, and simultaneously achieve blood glucose, uric acid detection and hematocrit correction.

The invention discloses a biological enzyme electrode, a preparation method thereof and a wearable electrochemical biosensor, and relates to the technical field of sensors. The bio-enzyme electrode comprises an electrode body, a bio-enzyme recognition layer arranged on the surface of the electrode body and an anti-interference layer arranged on the surface of the bio-enzyme recognition layer, the anti-interference layer adopts a molecularly imprinted polymer film, and adsorption sites matched with a target interferent structure are formed in the molecularly imprinted polymer film. The adsorption site is configured to adsorb a target interferent. According to the technical scheme, the anti-interference performance can be improved, and therefore the accuracy of the detection result of the target to-be-detected object is improved.

The purpose of the present invention is to provide a means with which it is possible to electrochemically measure a coenzyme in real time. Provided is an enzyme electrode including an electrode base material and a formic acid dehydrogenase β subunit (FoDH1B) fixed to the electrode base material via at least one compound selected from the group consisting of (1) and (2): (1) aromatic compounds having an aromatic hydrocarbon ring skeleton or a nitrogen-containing heterocyclic skeleton; and (2) compounds represented by general formula (2a). Formula 2a: HS-(CH2)m-OH [where m is an integer of 1-10].

This invention relates to a solid-liquid-gas three-phase interface enzyme electrode based on an iron oxide nanowire array and its application in biosensors. The iron oxide nanowire array grown on a titanium mesh substrate serves both as a superhydrophobic three-phase interface construction support and as a catalyst for the electroreduction of hydrogen peroxide, selectively catalyzing the reduction of hydrogen peroxide, a product of enzymatic reactions. The bio-enzyme layer is used to oxidize the analyte to generate hydrogen peroxide. A solid-liquid-gas three-phase interface enzyme electrode was successfully fabricated by loading oxidase onto a superhydrophobic iron oxide nanowire array. Oxygen from the air can be directly transported to the active sites of the enzyme catalytic reaction through the pores of the titanium mesh substrate, solving the problem of "oxygen deficiency" caused by dissolved oxygen in water in traditional two-phase interfaces, improving enzyme reaction kinetics, and significantly increasing the linear range for detecting the analyte.

To provide a biosensor capable of measuring amino acid concentration in a solution with higher accuracy. [Solution] A biosensor comprising an enzyme electrode including a conductive layer and a reagent layer adjacent to the conductive layer, wherein the conductive layer contains a mediator, and the reagent layer contains an amino acid oxidase and a cured product of a photosensitive polymer.

The invention belongs to the technical field of bioelectrocatalytic reduction of CO2, and relates to a charcoal-based enzyme electrode for CO2 reduction as well as a preparation method and application of the charcoal-based enzyme electrode. Firstly, SBA-15 is used as a template, microcrystalline cellulose is used as a carbon source, ordered porous biochar is constructed through temperature programming carbonization and template removal with strong base, then copper nanoparticles are formed in a CuSO4 electrolyte through cyclic voltammetry electrodeposition, and formate dehydrogenase and carbonic anhydrase are synergistically fixed on the surface of an electrode. The porous biochar provides a mass transfer channel with a high specific surface area and high efficiency, the copper nanoparticles improve the electron transfer efficiency, and formate dehydrogenase and carbonic anhydrase are combined to simulate a microbial carbon concentration mechanism, so that the CO2 concentration and reduction specificity of an interface are improved. Under the voltage of-0.3 V to-0.8 V (vs Ag / AgCl), CO2 is selectively reduced into formic acid, the process conditions are mild, the cost is low, and a feasible strategy is provided for high-selectivity formic acid preparation and CO2 recycling.