42 results about "Enzyme electrode" patented technology

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The invention discloses a dynamic blood glucose monitoring and intelligent pump speed adjusting system and method, and relates to the technical field of biomedical engineering.The dynamic blood glucose monitoring and intelligent pump speed adjusting method comprises the steps that precise monitoring is conducted through a multi-mode sensing module, an enzyme electrode sensor adopts an anti-interference coating, a Raman spectrum dynamic correction algorithm is combined, and motion artifacts and temperature drift are restrained; the metabolic feature fusion calibration module is integrated with a time convolution network, time sequence modeling is carried out on diet, exercise and historical blood glucose data, an individualized metabolic parameter database is constructed, and self-adaptive signal drift compensation is achieved; the self-adaptive pump control module innovatively designs a double-layer fuzzy PID controller, a base layer generates an infusion rate according to a real-time blood glucose state and insulin activity, an optimization layer introduces an LSTM metabolism prediction model, and the slope of an infusion curve is dynamically adjusted in combination with future behavior pre-judgment; in the edge-cloud collaborative architecture, a lightweight TCN model is locally deployed to guarantee real-time control, and a cloud end optimizes a global digital twinborn model through federal learning and generates a multi-dimensional metabolic risk early warning report.

The invention relates to a soil pesticide residue pollution rapid detection device and method.The soil pesticide residue pollution rapid detection device comprises a sample containing bottle, an oscillation filtering assembly, a turnover discharging assembly and a detector, the device integrates the functions of oscillation filtering, turnover discharging, enzyme electrode sensing detection and the like, and through circular motion and wave band oscillation of the oscillation filtering assembly, the soil pesticide residue pollution can be rapidly detected. The discharging operation of the overturning discharging assembly is combined, so that efficient extraction, filtration, separation and discharging of samples are realized; in addition, due to the design of the separated sample containing bottle, the sample treatment accuracy is further improved; finally, pesticide residues in the soil are rapidly and accurately detected by virtue of the sensitivity of the enzyme electrode sensor to the pesticide, and the requirement of on-site instant detection is met.

The invention discloses an enzyme electrode method efficient blood glucose measuring device for real-time monitoring of diabetes, and relates to the technical field of blood glucose detection. The enzyme electrode method efficient blood glucose measuring device for real-time monitoring of diabetes mellitus comprises a detection box main body, a detection cavity is formed in the detection box main body, a detection opening used for stretching of the arm of a patient is formed in one side of the detection box main body, and a placement module is arranged in the detection cavity and used for bearing the arm, stretching into the detection opening, of the patient; a pressing limiting structure is arranged at the upper end of the placement module, and the bottom of the detection box main body is hollow and is provided with a supporting unit capable of being quickly opened and closed. According to the blood glucose measuring device, the blood sampling mode of the vein blood vessel of the arm of a patient is adopted to replace fingertip blood sampling, blood sampling obtained through fingertip blood sampling is capillary whole blood and contains tissue fluid, the measuring result is lower than the actual value, and the effect of venous blood sampled through the vein blood vessel is more accurate; the system is more suitable for real-time monitoring of patients in daily life.

The invention provides an enzyme activity double-channel detection device in a high-pressure environment, which comprises a detection shell, a feeding port is downwards formed in the upper end of the detection shell, a reaction cavity is downwards formed in the bottom of the feeding port of the detection shell, the feeding port is detachably connected with a sealing cover, the sealing cover is connected with a sensor insertion shaft extending into the reaction cavity, and the sensor insertion shaft is connected with a sensor. A temperature sensor, a hydraulic sensor and an immobilized enzyme electrode are arranged at the free end of the sensor insertion shaft; a pressure pump is arranged outside the detection shell, and the outlet end of the pressure pump is communicated with the upper part of the reaction cavity through a pressure pipe; observation openings are inwards formed in the two sides of the detection shell, a transparent glass window is arranged on the side wall, at each observation opening, of the reaction cavity, a microscope lens group is arranged in each observation opening, and a spectrophotometer used for covering one observation opening is arranged on one side of the detection shell. According to the invention, enzyme activity determination can be carried out in a high-pressure environment, and the understanding of an adaptation mechanism of life under extreme conditions is greatly promoted.

The present disclosure relates to a biosensor comprising an enzyme electrode comprising a direct electron transfer type oxidoreductase wherein said enzyme electrode is electrically connected to a gate electrode of a field effect transistor (FET), and a method of measuring a substance using the biosensor.

The invention discloses a carbon material modified epinephrine photo-thermal enzyme electrode, a preparation method and application, and relates to the technical field of biosensing, the preparation method of the enzyme electrode comprises the following steps: S1, mixing citric acid, ethanediamine and formamide for reaction, filtering, purifying and centrifuging to prepare a carbon nano network solution; s2, sequentially adding gold trichloride and a reducing agent into the carbon nano network solution for reaction to prepare a carbon material loaded with gold nanoparticles; s3, dispersing the carbon material in a thiohydracrylic acid aqueous solution, then adding N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide, and carrying out an activation reaction, so as to prepare a functionalized carbon material; and S4, mixing the functionalized carbon material with a laccase solution, heating in a water bath, and dispensing the mixed solution on the surface of the electrode to prepare the epinephrine photo-thermal enzyme electrode. The prepared high-sensitivity photo-thermal laccase electrode has the advantages of simple method, mild conditions, wide detection limit, high sensitivity, good stability and the like.

A biofuel cell generates electricity by an oxidation-reduction reaction catalyzed by an enzyme, wherein the enzyme is immobilized on at least one of an anode and a cathode, the anode comprises a conductive material (1) and an electrode binder (1), the cathode comprises a conductive material (2) and an electrode binder (2), at least one of the electrode binder (1) and the electrode binder (2) comprises a polymer (A), the polymer (A) comprises a first structural unit derived from a nonionic ethylenically unsaturated monomer (a1), a second structural unit derived from an anionic ethylenically unsaturated monomer (a2), and a third structural unit derived from a crosslinking agent (a3), and the crosslinking agent (a3) has two or more ethylenically unsaturated bonds in one molecule.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The invention discloses a dopamine photothermal enzyme electrode modified with magnetic nanoparticles, a preparation method and an application thereof. First, chitosan-coated Fe3O4 nanoparticles are synthesized, and then gold nanoparticles are deposited. The obtained magnetic nanoparticles Fe3O4@CS-Au have excellent biocompatibility and conductivity; mercaptopropionic acid is used to covalently bind laccase on the surface of Fe3O4@CS-Au nanoparticles, and then the immobilized laccase is drop-coated on a glassy carbon electrode to prepare a dopamine photothermal sensor enzyme electrode modified with magnetic nanoparticles. Under light irradiation, the surface temperature of the magnetic nanoparticles increases, which can increase the catalytic activity of laccase in oxidizing dopamine, thereby increasing the response current of the laccase-modified electrode. Compared with the prior art, the enzyme electrode modified with magnetic nanoparticles of the present invention has the advantages of simple preparation method and mild conditions; the enzyme electrode has the advantages of wide detection range, high sensitivity, good stability, etc., and the enzyme electrode can function efficiently under light conditions, expanding its application in the fields of medical detection, food safety, etc.

To provide a biosensor capable of more accurately measuring the concentration of amino acids in a sample solution.SOLUTION: The present disclosure, in one aspect, relates to a biosensor including an enzyme electrode including a conductive layer containing a conductive material and a reagent layer adjacent to the conductive layer, the reagent layer including an amino acid oxidase and a polymer-type enzyme immobilizer. The polymer-type enzyme immobilizer is preferably a hydrogel.SELECTED DRAWING: Figure 1

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The present disclosure provides, among other features, an enzyme electrode in which an enzyme is immobilized via more appropriate bonds. An enzyme electrode according to the present disclosure includes an electrode and a modified protein having an amino acid sequence obtained by substituting at least one lysine residue in a fusion protein between a thioredoxin reductase and a thioredoxin with a non-lysine amino acid residue, in which the modified protein is immobilized on a surface of the electrode.

The present invention provides an immobilized enzyme electrode, an immobilized enzyme sensor and a detection method for anti-interference of an enzyme membrane, belonging to the technical field of biosensors. In the immobilized enzyme electrode of the present invention, it includes a detection electrode and an auxiliary electrode; the auxiliary electrode includes a basic electrode, an immobilized inactivated enzyme layer and a carrier membrane connected in sequence, and the enzyme immobilized in the immobilized inactivated enzyme layer is an inactivated enzyme. When measuring a sample in the present invention, the reference values of the detection electrode and the auxiliary electrode are tested, and the fluctuations and changes of the signals caused by interference are corrected simultaneously by the detection electrode and the auxiliary electrode, thereby avoiding the interference of the electrode signals caused by the "integrity" problem of the enzyme membrane and improving the measurement accuracy.

The invention discloses a microneedle array electrochemical sensor for in-situ detection of sepsis marker lactic acid, which comprises a working electrode, a reference electrode and a counter electrode which are arranged on a light-cured resin base, and the three electrodes all take epoxy resin as a matrix and dichloromethane as a dispersing solvent, and the working electrode, the reference electrode and the counter electrode are arranged on the light-cured resin base. A microneedle conductive layer is prepared from a conductive material prepared from composite multi-walled carbon nanotubes, light-cured resin is used as an insulating layer, a working electrode is provided with a lactic acid enzyme electrode modification layer, polyvinylpyrrolidone is used as a protective layer, in-situ detection of lactic acid in interstitial fluid is realized, and the microneedle array electrochemical sensor for in-situ detection of sepsis marker lactic acid is constructed. The kit has the advantages of being easy to process, short in processing period, low in cost, easy to realize large-scale production, high in sensitivity, strong in specificity and the like, can be used for clinical accurate, efficient and rapid sepsis diagnosis and real-time disease course monitoring, and also provides a new thought for biomarker detection of other diseases.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

In one aspect, the present disclosure provides a biosensor capable of providing a highly accurate measurement of the concentration of an amino acid in a solution to be measured. In one aspect, the present disclosure pertains to a biosensor comprising an enzyme electrode that includes a conductive layer containing a conductive material and a reagent layer adjacent to the conductive layer. The reagent layer contains an amino acid oxidase and a polymeric enzyme immobilization agent. The polymeric enzyme immobilization agent is preferably a hydrogel.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The present disclosure provides, among other features, an enzyme electrode in which an enzyme is immobilized via more appropriate bonds. An enzyme electrode according to the present disclosure includes an electrode and a modified protein having an amino acid sequence obtained by substituting at least one lysine residue in a fusion protein between a thioredoxin reductase and a thioredoxin with a non-lysine amino acid residue, in which the modified protein is immobilized on a surface of the electrode.

The present disclosure provides, among other features, an enzyme electrode in which an enzyme is immobilized via more appropriate bonds. An enzyme electrode according to the present disclosure includes an electrode and a modified protein having an amino acid sequence obtained by substituting at least one lysine residue in a protein that is a reductase or a dehydrogenase with a non-lysine amino acid residue, in which the modified protein is immobilized on a surface of the electrode.

A biosensor comprising an enzyme electrode that contains a conductive layer and a reagent layer adjacent to the conductive layer, wherein the conducive layer contains a mediator, and the reagent layer contains an amino acid oxidase and a cured product of a photosensitive polymer.

The invention relates to the field of electrochemical biosensors, in particular to a crosslinking process method of an enzyme membrane in a biosensor and a preparation method of an enzyme electrode. A cross-linking process method of an enzyme membrane in a biosensor comprises the following steps: spraying a cross-linking solution to the surface of the enzyme membrane, so that the fogdrop-shaped cross-linking solution permeates from the surface of the enzyme membrane to the inside of the enzyme membrane, and realizing different cross-linking densities of the inside and the outside of the enzyme membrane under the support of a polymer during incubation to obtain an enzyme electrode. According to the cross-linking process method of the enzyme membrane, the cross-linking reagent is atomized and evenly arranged on the enzyme membrane in a spraying mode, the prepared enzyme electrode is high in sensitivity and good in stability, meanwhile, operation is easy, batch production is easy, and the production cost is low.

The present invention belongs to the field of immobilized enzyme technology, and specifically relates to an immobilized enzyme electrode and its preparation method and application. The immobilized enzyme electrode provided by the present invention comprises a conductive carrier and a ferredoxin-NADP+ reductase and a reaction enzyme loaded on the conductive carrier; the conductive carrier comprises a conductive substrate and a porous graphene layer arranged on the surface of the conductive substrate, and the pore structure of the porous graphene layer is nanopores; the ferredoxin-NADP+ reductase and the reaction enzyme are loaded in the pore structure of the porous graphene layer. The immobilized enzyme electrode provided by the present invention can realize NADPH cyclic regeneration under the condition of an external power supply, ensure that the enzyme cascade reaction is continuous and stable; and the immobilized enzyme electrode can be used repeatedly, effectively reducing the cost of the enzyme cascade reaction, and is suitable for industrial expansion and application.

The application belongs to the technical field of electrochemical detection and analysis of stress biomarkers, and relates to an MBAzy heterojunction nanoscale enzyme, an electrode system, a flexible sensor and a detection method. 4 / 3 The MBAzy heterojunction nanoscale enzyme is a composite material of a single-atom nanoscale enzyme CuPC and MBene, the MBene is Mo B2MBene, and the mass ratio of the CuPC to the MBene is 10-20:1.The MBAzy heterojunction nanoscale enzyme can catalyze dopamine to generate electrons and cause an electrochemical reaction. The method based on the MBAzy heterojunction nanoscale enzyme can sensitively detect dopamine in a life flowing body.

The intelligent blood glucose monitoring device comprises a shell, a protection plate is hinged to the upper end of the shell, an enzyme electrode sensor is arranged in the middle of the bottom wall of the shell, fixing bandages are rotationally connected to the middles of the left end and the right end of the shell, and the intelligent blood glucose monitoring device further comprises a connecting mechanism and a fixing mechanism; the connecting mechanism comprises a socket, an inserting rod, a mounting seat, a connecting seat and driving plates, the socket is arranged on the lower side of the right end of the fixing bandage on the left side, the inserting rod is arranged on the right side in the socket, the connecting seat is slidably connected into a sliding groove formed in the middle of the bottom wall of the socket, and the driving plates are rotatably connected to the upper sides of the front end and the rear end of the connecting seat; according to the intelligent blood glucose monitoring device for gestational diabetes, the problem that the fixing bandage is loosened due to the fact that the magnet is pulled open accidentally is solved, the fixing effect is further improved, the operation process is simplified, and the use convenience is further improved.