186 results about "Enzyme electrode" patented technology

A biosensor comprises a space part for sucking and housing a sample formed of two upper and lower plates, the two plates being stuck together by an adhesive layer, the space part for sucking and housing the sample being constituted so as to be partially opened in the peripheral part and partially closed by the adhesive layer, and has a working electrode having at least glucose oxidase immobilized thereon and a counter electrode on the same plane of the plate.

A biosensor comprises a space part for sucking and housing a sample formed of two upper and lower plates, the two plates being stuck together by an adhesive layer, the space part for sucking and housing the sample being constituted so as to be partially opened in the peripheral part and partially closed by the adhesive layer, and has a working electrode having at least glucose oxidase immobilized thereon and a counter electrode on the same plane of the plate.

A non-mediated enzyme electrode comprises a base substrate (2) on which is provided an electrically conductive base layer (8) comprising finely divided platinum group metal or oxide bonded together by a resin; a top layer on the base layer (8), the top layer comprising a buffer. A catalytically active quantity of an oxidoreductase enzyme is provided in at least one of the base layer and the top layer. The invention also provides a biosensor (20) which includes an enzyme electrode, and methods of manufacturing the enzyme electrode and biosensor.

There is provided with an enzyme electrode which can be used as a sensor with high sensitivity, a biofuel cell with high output, and an electrochemical reaction device with high reaction efficiency. The enzyme electrode has a conductive base plate, a fusion protein immobilized to the conductive base plate and an electron transfer mediator, wherein the fusion protein is a fusion protein of a enzyme 1 to catalyze a chemical reaction for producing a reaction product 1 from a reaction substrate 1 and a enzyme 2 to catalyze a chemical reaction for producing a reaction product 2 from a reaction substrate 2, and at least one chemical substance of the reaction product 1 is identical to at least one chemical substance of the reaction substrate 2.

On substrate of electrode made from organic insulating material, conducting material is printed through spraying method so as to form working electrode and basal body, and a layer of polycarbonate insulator is coated on surfaces of intermediate part between them. Lower part of basal body of working electrode is coated a conversion zone formed through integrated application including techniques of high polymer built up film or ferrocene covering, embedding enzyme, nano technique, and diffusing astriction so as to fabricate biologic enzyme electrode. The invention raises each performance of biologic sensor and practicability integrally.

The invention provides an enzyme electrode usable as a highly sensitive sensor, a high-output bio fuel cell or an electrochemical reaction device of a high reaction efficiency. The enzyme electrode includes an electroconductive base member and an enzyme, wherein the enzyme is formed by an associated protein in which two or more different enzyme proteins are associated.

The invention discloses an enzyme electrode which belongs to the technical field of biosensors. The enzyme electrode consists of a base electrode and a responding layer fixed on the surface of the base electrode, wherein the responding layer consists of doped semiconductor nanometer wires on which enzyme is fixed. The invention also discloses a preparation method of the enzyme electrode. The preparation method comprises the step of adopting the doped semiconductor nanometer wires to fix enzyme molecules so as to achieve the direct electrochemical performance of the enzyme molecules. The enzyme electrode has better catalytic activity and wider linear range.

The present invention relates to an enzyme electrode for transporting electric charge formed by an oxidation-reduction reaction in an enzyme to a conductive member at a high speed. The enzyme electrode specifically has a π-conjugated metal complex molecule immobilized on the conductive member and the enzyme immobilized on the conductive member through the π-conjugated metal complex molecule.

The present invention provides an enzyme electrode exhibiting good measurement performance under wide ranges of the application conditions, being excellent in durability during long-term use and further being producible with a higher yield, as well as a process for manufacturing the enzyme electrode employing a wafer process particularly suitable to mass production. An enzyme electrode according to the present invention comprises an electrode 2 formed on an insulating substrate 1, an immobilized enzyme layer 4 formed over the electrode 2, and a permeation-limiting layer 6 placed on the uppermost surface and over the immobilized enzyme layer 4, wherein on the immobilized enzyme layer 4 is optionally formed an adhesion layer 8 comprising a silane-containing compound, on whose upper surface is formed the permeation-limiting layer 6; or the permeation-limiting layer 6 may be a film mainly comprising a fluorine-containing polymer in which a number of grooves are built on its surface, or alternatively the film has an irregular surface having a surface roughness of 0.0001 or more and 1 or less fold to its average thickness being selected within a range of 0.01 to 1 mum.

The invention provides a preparation method and an application of a co-immobilized enzyme electrode which is used for detecting p-dihydroxybenzene and o-dihydroxybenzene. The preparation method comprises steps of: immobilizing tyrosinase and peroxidase on oxidized grephene to obtain a co-immobilized enzyme liquid and smearing dropwisely the co-immobilized enzyme liquid and a chitosan solution successively on a surface of a glassy carbon electrode to obtain the co-immobilized enzyme electrode which is used for detecting p-dihydroxybenzene and o-dihydroxybenzene. The electrode is low in cost, is easy and convenient to operate, is quick and sensitive, is low in detection limit, is good in selectivity and can be used for detecting microscale p-dihydroxybenzene and o-dihydroxybenzene in environment.

An enzyme electrode has a conductive member and an enzyme, wherein the conductive member has a porous structure, and the enzyme is immobilized through a carrier in pores constituting the porous structure. An enzyme electrode device, comprises the enzyme electrode, and wiring connected to the conductive member of the enzyme electrode.

Disclosed is an enzyme electrode having an oxidoreductase (for instance, glucose oxidase, cholesterol oxidase, fructosylamine oxidase and glucose dehydrogenase) and an electron-transfer protein (for instance, cytochrome C, cytochrome b562 and cytochrome c551), as well as a sensor utilizing the enzyme electrode as working electrode. The enzyme electrode of the invention can provide high response current values.

The invention relates to an enzyme electrode, an enzyme biosensor as well as preparation methods and application of the enzyme electrode and the enzyme biosensor. The enzyme electrode comprises a base electrode, wherein a carbonized eggshell membrane is attached on the surface of the base electrode; nano-metal particles and enzyme are cured on the carbonized eggshell membrane. According to the enzyme electrode, a composite material of the eggshell membrane and the nano-metal particles is used as a carrier, enzyme molecules are effectively fixed and direct electron transfer between enzyme and the electrode is realized, thereby improving the catalytic activity of the enzyme molecules and the sensitivity of the sensor. Compared with the prior art, the enzyme electrode has the advantages that the eggshell membrane is used as a material so that reutilization of wastes is realized, and the preparation method is simple and low in cost, and electron transfer rate of the electrode and the enzyme is high; the enzyme biosensor is fast and accurate and high in detection sensitivity, stability and repeatability.

The invention relates to an enzyme electrode for detecting sterigmatocystin and a preparation method thereof, and a biosensor which uses the enzyme electrode as a substrate electrode for fixing and assembling aflatoxin oxidase and is used for detecting sterigmatocystin. An L-cysteine functional film is self-assembled on the surface of a gold electrode, and then the gold electrode is immersed into heterozygous Prussian-blue electrode-position working solution to prepare the Prussian-blue heterozygous electrode by a potentiostatic method. CNT (carbon nano tubes) used as electron carriers are evenly dispersed in chitosan solution and are used as carriers for embedding aflatoxin oxidase. Then the chitosan- aflatoxin oxidase-CNT mixed film is assembled on the surface of the Prussian-blue heterozygous modified electrode, and finally, the biosensor used for detecting sterigmatocystin is obtained. The enzyme electrode and the biosensor provided by the invention have better stability and repeatability, quick response to sterigmatocystin, low voltage detection, strong antijamming capacity and excellent selectivity.

The invention discloses a method for preparing an enzyme electrode with an MWCNTs-TiO2 / Nafion composite medium. In the method, MWCNTs-TiO2 core-shell nanocomposite materials are scattered into Nafion to prepare an organic-inorganic composite membrane as a fixed matrix for biological molecules for structuring a hemoglobin electrode, and a hemoglobin biosensor with fast response, high sensitivity and strong catalytic capacity is prepared by using the properties of large specific surface area, high surface reactivity, strong adsorption capacity, large electrical conductivity and the like of the MWCNTs-TiO2 core-shell nanocomposite materials and the properties of membrane forming, high chemical stability, high anti-interference capacity and the like of the Nafion. The biosensor has good biocompatibility, stability and repeatability, and has potential application in structuring biosensors. The sensor can be used for detecting the substances of hydrogen peroxide, trichloroacetic acid and the like, and has the advantages of low sensitivity, low detection limit and the like.

The invention belongs to the field of food detection, and particularly relates to a method for preparing an enzyme electrode and rapidly detecting the peroxide value of vegetable oil. The method comprises the steps of: selecting a glassy carbon electrode for use; firstly preparing a Nafion-methylene blue membrane electrode, then adopting a bovine serum albumin-glutaraldehyde crosslinking method to fix horse radish peroxidase (HRP), and obtaining a final HRP electrode; and then using the HRP electrode to rapidly detect the peroxide value of the vegetable oil. The method is high in sensitivity, good in selectivity, high in accuracy, short in response time, less in interference and superior to the traditional iodometry, thus being a simple, rapid, convenient and easy method for measuring the peroxide value and having the potential of realizing automatic on-site measurement.

The invention relates to a nanometer biology enzyme electrode which is formed by the tree-sharp macromolecular packaging mental nanometer and enzyme self-assembling. The nanometer particle is packaged inside the tree-sharp macromolecular to keep better activity; the electrode surface forms multi-foil layer with better scattering character and nanometer mental particle which can abridge the respond time and improve the sensing ability of the enzyme sensor. It can make super sensitive nanometer biology enzyme electrode by choosing different enzyme oxide needed by different applied.

The invention relates to a ZnO nanowire biosensor and a preparation method thereof, belonging to the technical field of nano semiconductor materials. In the prior art, the mixed solution of ZnO nano particles and enzyme are spun on an electrochemical electrode so that an enzyme electrode is made; the ZnO nano particles are fully wrapped by the non-conducting enzyme, and besides, an enzyme film formed on the surface of the electrochemical electrode is uneven, thereby affecting the direct electronic transfer between the active center of the enzyme and the electrode; and therefore, the sensitivity of the electrochemical electrode as a biosensor is reduced. The ZnO nanowire biosensor comprises a substrate, ZnO and the enzyme, wherein a conducting layer is arranged on the substrate, a ZnO nanowire is grown on the conducting layer, and an enzyme-wrapped layer is attached to the surface of the nanowire. The method of the invention comprises the following steps of: firstly making the conducting layer on the substrate, then growing the ZnO nanowire on the conducting layer, and thirdly generating the enzyme-wrapped layer on the surface of the nanowire. The biosensor as a working electrode is used for electrochemical working stations as chemical test devices to realize accurate tests.

An enzyme electrode that enables to enhance the rate of charge transfer from a redox center of an enzyme and catalytic current is provided. An enzyme electrode capable of increasing catalytic current by increasing the rate of charge transfer from an enzyme using an enzyme / metal fine particle complex in which part of a metal fine particle is incorporated into the enzyme can be provided.

An enzyme electric couple catalyzing method for treating waste water containing phenol, aromatic amine and azo-dye is carried out by fixing horse-radish peroxidase or analog enzyme with enzyme activity on grinded pyrolyzed graphite electrode surface by surface activator and carboxy-carbon nano-pipe, making into solid-carried enzyme or analog enzyme electrode, taking solid-carried enzyme or analog enzyme electrode as cathode, taking graphite electrode as anode, pumping waste water containing phenol, aromatic amine and azo-dye into cathode chamber, inducing oxygen and air into cathode chamber to saturate, controlling cathode electric potential at -1.00--0.500V vs.SCE by constant potentiometer and constant-potential electrolyzing. It has fast degradation speed and high current efficiency.

The invention provides an oxygen-enriched nano bio-enzyme electrode as well as a sensor device and a preparation method and application of oxygen-enriched nano bio-enzyme electrode. According to the oxygen-enriched nano bio-enzyme electrode, an electrode substrate is modified with a hollow structure nano material with the oxygen enrichment function, catalyst particles of hydrogen peroxide and an oxidase corresponding to a substance to be detected. The oxygen-enriched nano bio-enzyme electrode and the device have the advantages that the detection linear range is wide, the sensitivity is high, the anti-interference performance is good, and in addition, batch production can be carried out; and the advantages are mainly derived from the hollow structure material with the oxygen storage function.

The enzyme electrode test strip belongs to the field of bio-sensing technology, on the base sheet of test strip a working electrode, a reference electrode, an electric circuit and a conductor connected with reading apparatus, on the working electrode and reference electrode the reaction working liquor can be spray-coated, and the composition of said reaction working liquor includes (wt%) 0.8-1.2%of enzyme, 15-25% of conductive medium, 5-15% of adhesive and 60-80% of buffer agent. If said invented test strip is contacted with body fluid of human body, the tested result can be directly displayed on the correspondent matched apparatus.

The invention relates to a production method of a biological enzyme electrode used for the detection of pesticide residues, which pertains to the biochemistry electrodes, the production method adopts a combination method of nanoparticles and layer-by-layer self-assembly technology, the method has the steps that: a rod-shaped electrode carrier is made by using organic insulation materials, the substrate conductive materials are coated on a cross section of the lower end of the electrode carrier to form an electrode or the similar electrode is bought directly, then the outer surface of the conductive materials of the electrode uses the nanoparticles to decorate the electrode to form a nanoparticle layer, an anti-interference layer is prepared by the layer-by-layer self-assembly, an electrostatic adsorptive enzyme layer is prepared by the layer-by-layer self-assembly, so as to obtain a reaction layer which comprises the nanoparticle layer, the anti-interference layer and the electrostatic adsorptive enzyme layer, and the biological enzyme electrode used for detection of the pesticide residues is produced further. The production method of the invention is simple; a biosensor which is assembled by the biological enzyme electrode which is produced by the method has high sensitivity, stable performance and strong anti-interference capacity, so the invention is applicable to the actual application of the industrialization of the biological sensors for the detection of the pesticide residues.

The invention relates to a current enzyme electrode for detecting catalase-positive bacteria and a preparation method thereof in the technical field of biological sensing; the electrode comprises an electrode substrate, wiring terminals, electrode connection cables, a working electrode, a counter electrode, an insulation layer and reaction substrate films, and is characterized in that the wiring terminals, the electrode connection cables, the working electrode and the counter electrode are respectively arranged between the electrode substrate and the insulation layer; the working electrode is arranged in the middle of the electrode with a gap left between; one end of each electrode connection cable is respectively connected with the working electrode and the counter electrode, and the other end thereof is respectively connected with the two wiring terminals; and the reaction substrate films are arranged above the working electrode and the counter electrode. The electrode which is prepared by the method has the advantages of high sensitivity and no need of bacterial culturing, can realize continuous detection of a plurality of samples by replacing different disposable electrode strips, is simple to operate, is easy for industrial popularization, and has important practical value in food bacterial pollution monitoring.

This invention relates to mutagenized redox oxidase enzymes used to design enzyme electrodes with improved interference characteristics in the presence of mediator and oxygen in the assay. This recombinant modified enzyme has enhanced capability to transfer electrons to redox mediator instead of its natural electron acceptors such as O2, NAD, NADP, etc., which will improve the assay performance with less interference and higher sensitivity.

A method for preparing carbon-radical glucose chip of disposable type includes applying PVC material as base material then making track on it and coating silver size on it in order to use said size as conduction material, printing prepared graphite ink on said silver size track to form printed carbon electrode, covering carbon electrode by silia gel rubber layer, making aqueous solution with glucose oxidase and dripping the mixture on surface of printed carbon electrode, using PVA as film substrate material to fix enzyme on surface of carbon electrode by mixing said substrate material with enzyme and electronic transmission media.

The invention provides an agar sugar water gel fixed enzyme electrode and the preparing method and application thereof, dissolving agar sugar of 0.4-0.8g into 100 mL of boiling water and preparing agar sugar water gel, mixing the agar sugar water gel with organic solvent, then mixing with enzyme solution and preparing mixed solution, drop-smearing the mixed solution on the surface of electrode substrate, drying at room temperature and obtaining the agar sugar fixed enzyme electrode. And the invention uses characters and good bioaffinity of agar sugar to prepare the agar sugar fixed enzyme electrode, which has a wide use range, and can be used in water phase, water-containing organic phase and organic phase and has biological catalytic activity, and has quick response, good stability and reproducibility, long service life, high accuracy and precision, and wide measuring range.

The invention discloses a film having protein adsorption resistance and a preparation method thereof. The film is a polymer of a zwitterionic monomer and covers the surface of an enzyme electrode. The film is prepared by polymerizing the zwitterionic monomer by virtue of electrically induced atom transfer free radical polymerization on the surface of an enzyme electrode. The film, which covers the surface of an enzyme electrode, has good protein adsorption resistance and meanwhile, the high-sensitivity detection through a sensor is ensured; an electrochemical means is fully utilized, the process is simple, the manufacturability is strong, the time is short and the film can be used for large-scale preparation; the thickness of the film can be controlled at 10-40 nm and good permeability is achieved; and at most 99% of non-specific protein adsorption can be reduced.

The invention provides a method for building a three-dimensional enzyme electrode surface by using coaxial nanofibers. The method comprises the following steps: preparing a coaxial electrospinning kernel material by dissolving a high molecular weight polymer and a high-orientation nano material into water; preparing a coaxial electrospinning shell material by dissolving enzymes or proteins with catalytic property and a high molecular weight polymer together into water; spinning nanofibers on an electrode surface by using a coaxial electrospinning method; and cross-linking by using a cross-linking agent to obtain a three-dimensional enzyme electrode. The conditions of the coaxial electrospinning method are that spinning voltage is 8-30kV, liquid flow rate is 0.5-2mL / h, and spinning interval is 10-100cm. According to the method, the coaxial electrospinning technology is used to prepare a coaxial nanofiber film electrode, the preparation of the enzyme electrode is finished by relatively simple steps, the surface enzyme immobilization stability of the enzyme electrode is improved, the response speed of the enzyme electrode is improved, and the output power of a fuel battery is increased.

The disclosure discloses an enzyme electrode comprising; an electrode comprising a current collector; a monolayer-forming molecule bound to the surface of the current collector; and a glucose dehydrogenase comprising a cytochrome C subunit bound to the monolayer-forming molecule; wherein electrons are transferred between the glucose dehydrogenase and the current collector by oxidation-reduction reaction of the glucose dehydrogenase.