185 results about "Enzyme electrode" patented technology

A biosensor comprises a space part for sucking and housing a sample formed of two upper and lower plates, the two plates being stuck together by an adhesive layer, the space part for sucking and housing the sample being constituted so as to be partially opened in the peripheral part and partially closed by the adhesive layer, and has a working electrode having at least glucose oxidase immobilized thereon and a counter electrode on the same plane of the plate.

A non-mediated enzyme electrode comprises a base substrate (2) on which is provided an electrically conductive base layer (8) comprising finely divided platinum group metal or oxide bonded together by a resin; a top layer on the base layer (8), the top layer comprising a buffer. A catalytically active quantity of an oxidoreductase enzyme is provided in at least one of the base layer and the top layer. The invention also provides a biosensor (20) which includes an enzyme electrode, and methods of manufacturing the enzyme electrode and biosensor.

There is provided with an enzyme electrode which can be used as a sensor with high sensitivity, a biofuel cell with high output, and an electrochemical reaction device with high reaction efficiency. The enzyme electrode has a conductive base plate, a fusion protein immobilized to the conductive base plate and an electron transfer mediator, wherein the fusion protein is a fusion protein of a enzyme 1 to catalyze a chemical reaction for producing a reaction product 1 from a reaction substrate 1 and a enzyme 2 to catalyze a chemical reaction for producing a reaction product 2 from a reaction substrate 2, and at least one chemical substance of the reaction product 1 is identical to at least one chemical substance of the reaction substrate 2.

On substrate of electrode made from organic insulating material, conducting material is printed through spraying method so as to form working electrode and basal body, and a layer of polycarbonate insulator is coated on surfaces of intermediate part between them. Lower part of basal body of working electrode is coated a conversion zone formed through integrated application including techniques of high polymer built up film or ferrocene covering, embedding enzyme, nano technique, and diffusing astriction so as to fabricate biologic enzyme electrode. The invention raises each performance of biologic sensor and practicability integrally.

The invention provides an enzyme electrode usable as a highly sensitive sensor, a high-output bio fuel cell or an electrochemical reaction device of a high reaction efficiency. The enzyme electrode includes an electroconductive base member and an enzyme, wherein the enzyme is formed by an associated protein in which two or more different enzyme proteins are associated.

The invention discloses an enzyme electrode which belongs to the technical field of biosensors. The enzyme electrode consists of a base electrode and a responding layer fixed on the surface of the base electrode, wherein the responding layer consists of doped semiconductor nanometer wires on which enzyme is fixed. The invention also discloses a preparation method of the enzyme electrode. The preparation method comprises the step of adopting the doped semiconductor nanometer wires to fix enzyme molecules so as to achieve the direct electrochemical performance of the enzyme molecules. The enzyme electrode has better catalytic activity and wider linear range.

The present invention relates to an enzyme electrode for transporting electric charge formed by an oxidation-reduction reaction in an enzyme to a conductive member at a high speed. The enzyme electrode specifically has a π-conjugated metal complex molecule immobilized on the conductive member and the enzyme immobilized on the conductive member through the π-conjugated metal complex molecule.

An enzyme electrode has a conductive member and an enzyme, wherein the conductive member has a porous structure, and the enzyme is immobilized through a carrier in pores constituting the porous structure. An enzyme electrode device, comprises the enzyme electrode, and wiring connected to the conductive member of the enzyme electrode.

The invention discloses a method for preparing an enzyme electrode with an MWCNTs-TiO2/Nafion composite medium. In the method, MWCNTs-TiO2 core-shell nanocomposite materials are scattered into Nafion to prepare an organic-inorganic composite membrane as a fixed matrix for biological molecules for structuring a hemoglobin electrode, and a hemoglobin biosensor with fast response, high sensitivity and strong catalytic capacity is prepared by using the properties of large specific surface area, high surface reactivity, strong adsorption capacity, large electrical conductivity and the like of the MWCNTs-TiO2 core-shell nanocomposite materials and the properties of membrane forming, high chemical stability, high anti-interference capacity and the like of the Nafion. The biosensor has good biocompatibility, stability and repeatability, and has potential application in structuring biosensors. The sensor can be used for detecting the substances of hydrogen peroxide, trichloroacetic acid and the like, and has the advantages of low sensitivity, low detection limit and the like.

The invention relates to a nanometer biology enzyme electrode which is formed by the tree-sharp macromolecular packaging mental nanometer and enzyme self-assembling. The nanometer particle is packaged inside the tree-sharp macromolecular to keep better activity; the electrode surface forms multi-foil layer with better scattering character and nanometer mental particle which can abridge the respond time and improve the sensing ability of the enzyme sensor. It can make super sensitive nanometer biology enzyme electrode by choosing different enzyme oxide needed by different applied.

The invention relates to a ZnO nanowire biosensor and a preparation method thereof, belonging to the technical field of nano semiconductor materials. In the prior art, the mixed solution of ZnO nano particles and enzyme are spun on an electrochemical electrode so that an enzyme electrode is made; the ZnO nano particles are fully wrapped by the non-conducting enzyme, and besides, an enzyme film formed on the surface of the electrochemical electrode is uneven, thereby affecting the direct electronic transfer between the active center of the enzyme and the electrode; and therefore, the sensitivity of the electrochemical electrode as a biosensor is reduced. The ZnO nanowire biosensor comprises a substrate, ZnO and the enzyme, wherein a conducting layer is arranged on the substrate, a ZnO nanowire is grown on the conducting layer, and an enzyme-wrapped layer is attached to the surface of the nanowire. The method of the invention comprises the following steps of: firstly making the conducting layer on the substrate, then growing the ZnO nanowire on the conducting layer, and thirdly generating the enzyme-wrapped layer on the surface of the nanowire. The biosensor as a working electrode is used for electrochemical working stations as chemical test devices to realize accurate tests.

An enzyme electrode that enables to enhance the rate of charge transfer from a redox center of an enzyme and catalytic current is provided. An enzyme electrode capable of increasing catalytic current by increasing the rate of charge transfer from an enzyme using an enzyme/metal fine particle complex in which part of a metal fine particle is incorporated into the enzyme can be provided.

An enzyme electric couple catalyzing method for treating waste water containing phenol, aromatic amine and azo-dye is carried out by fixing horse-radish peroxidase or analog enzyme with enzyme activity on grinded pyrolyzed graphite electrode surface by surface activator and carboxy-carbon nano-pipe, making into solid-carried enzyme or analog enzyme electrode, taking solid-carried enzyme or analog enzyme electrode as cathode, taking graphite electrode as anode, pumping waste water containing phenol, aromatic amine and azo-dye into cathode chamber, inducing oxygen and air into cathode chamber to saturate, controlling cathode electric potential at -1.00--0.500V vs.SCE by constant potentiometer and constant-potential electrolyzing. It has fast degradation speed and high current efficiency.

The enzyme electrode test strip belongs to the field of bio-sensing technology, on the base sheet of test strip a working electrode, a reference electrode, an electric circuit and a conductor connected with reading apparatus, on the working electrode and reference electrode the reaction working liquor can be spray-coated, and the composition of said reaction working liquor includes (wt%) 0.8-1.2%of enzyme, 15-25% of conductive medium, 5-15% of adhesive and 60-80% of buffer agent. If said invented test strip is contacted with body fluid of human body, the tested result can be directly displayed on the correspondent matched apparatus.

The invention relates to a production method of a biological enzyme electrode used for the detection of pesticide residues, which pertains to the biochemistry electrodes, the production method adopts a combination method of nanoparticles and layer-by-layer self-assembly technology, the method has the steps that: a rod-shaped electrode carrier is made by using organic insulation materials, the substrate conductive materials are coated on a cross section of the lower end of the electrode carrier to form an electrode or the similar electrode is bought directly, then the outer surface of the conductive materials of the electrode uses the nanoparticles to decorate the electrode to form a nanoparticle layer, an anti-interference layer is prepared by the layer-by-layer self-assembly, an electrostatic adsorptive enzyme layer is prepared by the layer-by-layer self-assembly, so as to obtain a reaction layer which comprises the nanoparticle layer, the anti-interference layer and the electrostatic adsorptive enzyme layer, and the biological enzyme electrode used for detection of the pesticide residues is produced further. The production method of the invention is simple; a biosensor which is assembled by the biological enzyme electrode which is produced by the method has high sensitivity, stable performance and strong anti-interference capacity, so the invention is applicable to the actual application of the industrialization of the biological sensors for the detection of the pesticide residues.

The invention relates to a current enzyme electrode for detecting catalase-positive bacteria and a preparation method thereof in the technical field of biological sensing; the electrode comprises an electrode substrate, wiring terminals, electrode connection cables, a working electrode, a counter electrode, an insulation layer and reaction substrate films, and is characterized in that the wiring terminals, the electrode connection cables, the working electrode and the counter electrode are respectively arranged between the electrode substrate and the insulation layer; the working electrode is arranged in the middle of the electrode with a gap left between; one end of each electrode connection cable is respectively connected with the working electrode and the counter electrode, and the other end thereof is respectively connected with the two wiring terminals; and the reaction substrate films are arranged above the working electrode and the counter electrode. The electrode which is prepared by the method has the advantages of high sensitivity and no need of bacterial culturing, can realize continuous detection of a plurality of samples by replacing different disposable electrode strips, is simple to operate, is easy for industrial popularization, and has important practical value in food bacterial pollution monitoring.

This invention relates to mutagenized redox oxidase enzymes used to design enzyme electrodes with improved interference characteristics in the presence of mediator and oxygen in the assay. This recombinant modified enzyme has enhanced capability to transfer electrons to redox mediator instead of its natural electron acceptors such as O₂, NAD, NADP, etc., which will improve the assay performance with less interference and higher sensitivity.

A method for preparing carbon-radical glucose chip of disposable type includes applying PVC material as base material then making track on it and coating silver size on it in order to use said size as conduction material, printing prepared graphite ink on said silver size track to form printed carbon electrode, covering carbon electrode by silia gel rubber layer, making aqueous solution with glucose oxidase and dripping the mixture on surface of printed carbon electrode, using PVA as film substrate material to fix enzyme on surface of carbon electrode by mixing said substrate material with enzyme and electronic transmission media.

The invention provides an agar sugar water gel fixed enzyme electrode and the preparing method and application thereof, dissolving agar sugar of 0.4-0.8g into 100 mL of boiling water and preparing agar sugar water gel, mixing the agar sugar water gel with organic solvent, then mixing with enzyme solution and preparing mixed solution, drop-smearing the mixed solution on the surface of electrode substrate, drying at room temperature and obtaining the agar sugar fixed enzyme electrode. And the invention uses characters and good bioaffinity of agar sugar to prepare the agar sugar fixed enzyme electrode, which has a wide use range, and can be used in water phase, water-containing organic phase and organic phase and has biological catalytic activity, and has quick response, good stability and reproducibility, long service life, high accuracy and precision, and wide measuring range.

The disclosure discloses an enzyme electrode comprising; an electrode comprising a current collector; a monolayer-forming molecule bound to the surface of the current collector; and a glucose dehydrogenase comprising a cytochrome C subunit bound to the monolayer-forming molecule; wherein electrons are transferred between the glucose dehydrogenase and the current collector by oxidation-reduction reaction of the glucose dehydrogenase.