20 results about "Enzyme electrode" patented technology

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

A biofuel cell generates electricity by an oxidation-reduction reaction catalyzed by an enzyme, wherein the enzyme is immobilized on at least one of an anode and a cathode, the anode comprises a conductive material (1) and an electrode binder (1), the cathode comprises a conductive material (2) and an electrode binder (2), at least one of the electrode binder (1) and the electrode binder (2) comprises a polymer (A), the polymer (A) comprises a first structural unit derived from a nonionic ethylenically unsaturated monomer (a1), a second structural unit derived from an anionic ethylenically unsaturated monomer (a2), and a third structural unit derived from a crosslinking agent (a3), and the crosslinking agent (a3) has two or more ethylenically unsaturated bonds in one molecule.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The present disclosure provides, among other features, an enzyme electrode in which an enzyme is immobilized via more appropriate bonds. An enzyme electrode according to the present disclosure includes an electrode and a modified protein having an amino acid sequence obtained by substituting at least one lysine residue in a protein that is a reductase or a dehydrogenase with a non-lysine amino acid residue, in which the modified protein is immobilized on a surface of the electrode.

A biosensor comprising an enzyme electrode that contains a conductive layer and a reagent layer adjacent to the conductive layer, wherein the conducive layer contains a mediator, and the reagent layer contains an amino acid oxidase and a cured product of a photosensitive polymer.

The application belongs to the technical field of electrochemical detection and analysis of stress biomarkers, and relates to an MBAzy heterojunction nanoscale enzyme, an electrode system, a flexible sensor and a detection method. 4 / 3 The MBAzy heterojunction nanoscale enzyme is a composite material of a single-atom nanoscale enzyme CuPC and MBene, the MBene is Mo B2MBene, and the mass ratio of the CuPC to the MBene is 10-20:1.The MBAzy heterojunction nanoscale enzyme can catalyze dopamine to generate electrons and cause an electrochemical reaction. The method based on the MBAzy heterojunction nanoscale enzyme can sensitively detect dopamine in a life flowing body.

The intelligent blood glucose monitoring device comprises a shell, a protection plate is hinged to the upper end of the shell, an enzyme electrode sensor is arranged in the middle of the bottom wall of the shell, fixing bandages are rotationally connected to the middles of the left end and the right end of the shell, and the intelligent blood glucose monitoring device further comprises a connecting mechanism and a fixing mechanism; the connecting mechanism comprises a socket, an inserting rod, a mounting seat, a connecting seat and driving plates, the socket is arranged on the lower side of the right end of the fixing bandage on the left side, the inserting rod is arranged on the right side in the socket, the connecting seat is slidably connected into a sliding groove formed in the middle of the bottom wall of the socket, and the driving plates are rotatably connected to the upper sides of the front end and the rear end of the connecting seat; according to the intelligent blood glucose monitoring device for gestational diabetes, the problem that the fixing bandage is loosened due to the fact that the magnet is pulled open accidentally is solved, the fixing effect is further improved, the operation process is simplified, and the use convenience is further improved.

The present invention relates to an enzyme electrode for glucose measurement. The enzyme electrode comprises an electrode substrate layer and a sensing layer covering the electrode substrate layer, wherein the sensing layer comprises: an NAD(P)(H)-dependent glucose oxidoreductase and an NAD(P)(H) oxidoreductase. The enzyme electrode further comprises a first polymer layer covering the sensing layer, the first polymer layer being used for protecting the sensing layer and / or restricting the permeation of glucose and / or an interferant. The enzyme electrode further comprises NAD(P)(H) which is located on the sensing layer; and / or an NAD(P)(H) derivative which is a polymer covalently bound to NAD(P)(H) and located on the sensing layer and / or the first polymer layer. The enzyme electrode of the present invention can achieve stable, accurate and / or anti-interference continuous measurement of the blood glucose level.

The purpose of the present invention is to provide a means with which it is possible to electrochemically measure a coenzyme in real time. Provided is an enzyme electrode including an electrode base material and a formic acid dehydrogenase β subunit (FoDH1B) fixed to the electrode base material via at least one compound selected from the group consisting of (1) and (2): (1) aromatic compounds having an aromatic hydrocarbon ring skeleton or a nitrogen-containing heterocyclic skeleton; and (2) compounds represented by general formula (2a). Formula 2a: HS-(CH2)m-OH [where m is an integer of 1-10].

This invention relates to a solid-liquid-gas three-phase interface enzyme electrode based on an iron oxide nanowire array and its application in biosensors. The iron oxide nanowire array grown on a titanium mesh substrate serves both as a superhydrophobic three-phase interface construction support and as a catalyst for the electroreduction of hydrogen peroxide, selectively catalyzing the reduction of hydrogen peroxide, a product of enzymatic reactions. The bio-enzyme layer is used to oxidize the analyte to generate hydrogen peroxide. A solid-liquid-gas three-phase interface enzyme electrode was successfully fabricated by loading oxidase onto a superhydrophobic iron oxide nanowire array. Oxygen from the air can be directly transported to the active sites of the enzyme catalytic reaction through the pores of the titanium mesh substrate, solving the problem of "oxygen deficiency" caused by dissolved oxygen in water in traditional two-phase interfaces, improving enzyme reaction kinetics, and significantly increasing the linear range for detecting the analyte.

To provide a biosensor capable of measuring amino acid concentration in a solution with higher accuracy. [Solution] A biosensor comprising an enzyme electrode including a conductive layer and a reagent layer adjacent to the conductive layer, wherein the conductive layer contains a mediator, and the reagent layer contains an amino acid oxidase and a cured product of a photosensitive polymer.

The invention belongs to the technical field of bioelectrocatalytic reduction of CO2, and relates to a charcoal-based enzyme electrode for CO2 reduction as well as a preparation method and application of the charcoal-based enzyme electrode. Firstly, SBA-15 is used as a template, microcrystalline cellulose is used as a carbon source, ordered porous biochar is constructed through temperature programming carbonization and template removal with strong base, then copper nanoparticles are formed in a CuSO4 electrolyte through cyclic voltammetry electrodeposition, and formate dehydrogenase and carbonic anhydrase are synergistically fixed on the surface of an electrode. The porous biochar provides a mass transfer channel with a high specific surface area and high efficiency, the copper nanoparticles improve the electron transfer efficiency, and formate dehydrogenase and carbonic anhydrase are combined to simulate a microbial carbon concentration mechanism, so that the CO2 concentration and reduction specificity of an interface are improved. Under the voltage of-0.3 V to-0.8 V (vs Ag / AgCl), CO2 is selectively reduced into formic acid, the process conditions are mild, the cost is low, and a feasible strategy is provided for high-selectivity formic acid preparation and CO2 recycling.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The application provides an oxygen-rich electrochemical enzyme sensor based on porous tin oxide and application. The construction of the working electrode of the oxygen-rich electrochemical enzyme sensor mainly comprises the following steps: firstly, preparing porous tin oxide on a conductive substrate; then, performing hydrophobic treatment on the porous tin oxide; and finally, modifying an oxidase layer on the top of the porous tin oxide to obtain an oxygen-rich enzyme electrode. The working electrode, a reference electrode and a counter electrode are assembled to obtain an electrochemical sensor. The sensor solves the problem of "oxygen deficiency" existing in an enzymatic reaction, avoids the synchronous reduction of oxygen in the process of electro-reduction detection, has a lower background current and a higher sensitivity, and can ensure the accuracy of detection of high-concentration and low-concentration to-be-detected substances.